Publications by authors named "Limin Zhang"

373 Publications

Effect of Adjusted Antiplatelet Therapy on Preventing Ischemic Events After Stenting for Intracranial Aneurysms.

Stroke 2021 Sep 20:STROKEAHA120032989. Epub 2021 Sep 20.

Department of Interventional Neuroradiology, Beijing Neurosurgical Institute, Beijing Tiantan Hospital, Capital Medical University, China. (W.L., W.Z., Yisen Zhang, K.W., Ying Zhang, C.W., J.L., X.Y.).

Background And Purpose: This study tests whether patients with unruptured intracranial aneurysm who underwent stent placement benefitted from platelet function monitoring-guided adjustment of antiplatelet therapy.

Methods: We conducted a randomized, open-label, parallel group, assessor-blinded trial. Patients with unruptured intracranial aneurysm who underwent stent placement were assigned in a 1:1 ratio to receive either drug adjustment (patients who had high on-treatment platelet reactivity to antiplatelet therapy on the basis of platelet function monitoring [monitoring group]) or conventional therapy (without monitoring and drug adjustment [conventional group]). The second monitoring was performed 14 days after randomization in patients with drug adjustment. The primary outcome was the composite frequency of ischemic stroke, transient ischemic attack, stent thrombosis, urgent revascularization, and cerebrovascular death within 7 days after stent implantation. The safety outcome was the composite frequency of major, minor, or minimal bleeding within 1 month after stent implantation.

Results: In total, 314 patients were included (n=157 per group). The primary combined outcome occurred in 19 patients (12.1%) in the conventional group and 8 patients (5.1%) in the monitoring group (hazard ratio, 0.39 [95% CI, 0.17-0.92]; =0.03). Ischemic stroke occurred at a lower frequency in the monitoring group compared with that in the conventional group (4.5% versus 12.1%; hazard ratio, 0.34 [95% CI, 0.14-0.83]; =0.01), which drove the overall primary combined outcome. The safety outcome occurred in the monitoring group (7.0%) and in the conventional group (1.9%; hazard ratio, 3.87 [95% CI, 1.06-14.14]; =0.03). A significant difference was observed in the frequency of minor or minimal bleeding events between the two groups (monitoring group versus conventional group, 6.4% versus 1.3%; =0.02) but not in the frequency of major bleeding events between the two groups.

Conclusions: Platelet function monitoring-guided antiplatelet therapy reduces thromboembolic events in patients with unruptured intracranial aneurysm after stent placement, significantly enhancing minor or minimal bleeding events but not major bleeding events. Registration: URL: https://www.clinicaltrials.gov; Unique identifier: NCT03989557.
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http://dx.doi.org/10.1161/STROKEAHA.120.032989DOI Listing
September 2021

Epidemiological features of hypertension among ischemic survivors in Northeast China: insights from a population-based study, 2017-2019.

BMC Public Health 2021 09 9;21(1):1648. Epub 2021 Sep 9.

Department of Ultrasound, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.

Background: Hypertension remains the major modifiable risk factor of stroke recurrence. The study aimed to determine the up-to-date epidemiological features of hypertension among the survivors of ischemic stroke.

Methods: Our cross-sectional study included 18,796 adults aged ≥40 years and residing in northeast China. Ischemic stroke was diagnosed according to the World Health Organization's criteria, which requires the clinical record, computed tomography (CT) and/or magnetic resonance imaging (MRI) during the hospital stay. Hypertension was defined according to the Chinese hypertension guidelines (mean SBP ≥140 mmHg and/or mean DBP ≥90 mmHg, and/or self-reported use of anti-hypertensive medication in the past 2 weeks).

Results: Of the 986 survivors of ischemic stroke, 819 (83.1%) were identified with hypertension (535 were pre-stroke hypertension and 284 were post-stroke hypertension). Among hypertensive patients, the awareness and treatment rates were 76.8 and 66.7% respectively. Only 11.0% achieved an appropriate blood pressure (< 140 mmHg and < 90 mmHg) among those who took hypertensive medications. 16.8% of treated hypertensive patients received combination therapy, and calcium channel blockers were the most frequently used anti-hypertensive medication as monotherapy. The mean systolic blood pressure (SBP) and diastolic blood pressure (DBP) of the stroke population was 155.3 ± 22.9 mmHg and 89.2 ± 12.3 mmHg. Both SBP and DBP were higher in rural patients than in urban patients (158.5 ± 23.8 mmHg vs. 146.4 ± 17.5 mmHg and 90.3 ± 12.9 mmHg vs. 85.9 ± 10.1 mmHg, respectively; p < 0.001). The rates of stage 2 and above hypertension in the ischemic stroke population were 32.5 and 18.7%, and was significantly higher in rural areas than in urban areas.

Conclusions: The prevalence of poorly-controlled hypertension and the high rates of blood pressures at stages 2 and above in patients with prior ischemic stroke demonstrated an alarming situation in northeast China.
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http://dx.doi.org/10.1186/s12889-021-11692-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427863PMC
September 2021

Co-utilization of lake sediment and blue-green algae for porous lightweight aggregate (ceramsite) production.

Chemosphere 2021 Sep 3;287(Pt 2):132145. Epub 2021 Sep 3.

School of Chemistry and Materials Science, School of Environment, Jiangsu Provincial Key Laboratory of Material Cycling and Pollution Control, Nanjing Normal University, Nanjing, 210046, China.

Lake sediment and algal sludge with large output posed significant environmental risks. In this work, an idea of co-utilization of both solid wastes for the production of ceramsite (a sort of porous lightweight aggregates as building materials) was proposed and validated for the first time. The treatment process contained a dewatering step by a flocculation-pressure filtration method, and a sintered ceramsite preparation step. Effects of flocculant type and dosage on the dewatering performance were studied in the first step. An environmental-friendly amphoteric starch flocculant with a dosage of 12 mg/(g dried sample) was found to achieve the best dewatering performance. Effects of raw material mass ratio, sintering temperature and time in the second step were investigated. Under the optimal conditions (60 wt% of dewatered sediment; 20 wt% of dewatered algal sludge; 20 wt% of additives (fly ash: calcium oxide: kaolin = 2:1:2); sintering temperature: 1100 °C; time: 35 min), the obtained ceramsite met the Chinese National Standard as a qualified building material, with reliable environmental safety according to the leaching results for both heavy metals and microcystins. Both environmental and economic benefits of the proposed treatment were assessed. The process completely followed the rules of "reduction, harmlessness and resource utilization" for solid waste treatment and disposal; Meanwhile, the profit of the proposed ceramsite production could be more than 2.3 US dollar/m. The co-utilization method in this work acted as a good example for the comprehensive management of solid wastes in water-rich areas.
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http://dx.doi.org/10.1016/j.chemosphere.2021.132145DOI Listing
September 2021

Optimal control of the compositions, interfaces, and defects of hollow sulfide for electromagnetic wave absorption.

J Colloid Interface Sci 2021 Sep 1;607(Pt 1):24-33. Epub 2021 Sep 1.

MOE Key Laboratory of Material Physics and Chemistry under Extraordinary, School of Physical Science and Technology, Northwestern Polytechnical University, Xi'an 710072, China; School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China. Electronic address:

The aimlessness in the selection of dielectric absorbing materials and the regulation of complex permittivity consumes time and resources. It is an effective way to construct electromagnetic wave (EMW)-absorbing materials dominated by dielectric loss to select materials and adjust complex permittivity based on theory. With sulfide as an example, a hollow ZnO/ZnS composite was constructed using ZnO as a hard template. Subsequently, based on the diverse binding ability of Cu and Zn ions to S ions, the compositions, interfaces, and defects of the sample were simultaneously regulated. There was competition and synergy between the relaxation process caused by the defects and interfaces and the conductivity loss, resulting in the regulation of complex permittivity. Furthermore, the hollow structure effectively reduced the density of the material and improved the impedance matching ability of the sample. As a result, the effective absorption bandwidth (EAB) of the hollow nanoflower ZnO/ZnS/CuS composite reached 5.2 GHz (from 12.8 to 18 GHz) with a matching thickness of 1.59 mm. This method provides a direction for ameliorating the complex permittivity of EMW-absorbing materials dominated by dielectric loss to realize broadband absorption.
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http://dx.doi.org/10.1016/j.jcis.2021.08.186DOI Listing
September 2021

Effects of 7,8-dihydroxycoumarin on the myelin morphological changes and PSD-95 protein expression in Balb/c mice after sciatic nerve injury.

Neuroreport 2021 Oct;32(14):1198-1205

Institute of Orthopedic Diseases, Affiliated Hospital of Chifeng University, Chifeng, China.

Objective: To investigate the effects of 7,8-dihydroxycoumarin on the myelin morphological changes and PSD-95 protein expression in mice with sciatic nerve injury, and to explore the relationship between PSD-95 protein and myelin regeneration after nerve myelin injury.

Methods: One hundred twenty-seven male adult Balb/c mice were selected and randomly divided into high, medium and low 7,8-dihydroxycoumarin dose groups and blank control group. Anastomosis was then carried out for the amputated right sciatic nerve, and intraperitoneal injection of 7,8-dihydroxycoumarin was applied postoperatively. At weeks 1, 2, 4 and 8 after surgery, nervous tissues from the injury side were taken for immunohistochemical Luxol Fast Blue staining, so as to observe the morphological changes of the locally injured nerve myelin. Meanwhile, PSD-95 mRNA and protein expression were determined using real-time PCR and western blotting.

Results: The nerve myelin recovery in injury side of mice at all time points showed a definite dose-effect relationship with the dose of 7,8-dihydroxycoumarin. Moreover, 7,8-dihydroxycoumarin could inhibit the PSD-95 mRNA level and protein expression. At the same time, there was a dose-effect of the inhibition.

Conclusions: 7,8-Dihydroxycoumarin can affect nerve recovery in mice with sciatic nerve injury, which shows a definite dose-effect relationship with its dose. Besides, PSD-95 protein expression can suppress the regeneration of the injured nerve myelin.
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http://dx.doi.org/10.1097/WNR.0000000000001712DOI Listing
October 2021

Three-dimensional printed hydroxyapatite bone tissue engineering scaffold with antibacterial and osteogenic ability.

J Biol Eng 2021 Aug 9;15(1):21. Epub 2021 Aug 9.

Department of Orthopedics, Affiliated Hospital of Chifeng University, Inner Mongolia, 024000, Chifeng, People's Republic of China.

The development of an effective scaffold for bone defect repair is an urgent clinical need. However, it is challenging to design a scaffold with efficient osteoinduction and antimicrobial activity for regeneration of bone defect. In this study, we successfully prepared a hydroxyapatite (HA) porous scaffold with a surface-specific binding of peptides during osteoinduction and antimicrobial activity using a three-dimensional (3D) printing technology. The HA binding domain (HABD) was introduced to the C-terminal of bone morphogenetic protein 2 mimetic peptide (BMP2-MP) and antimicrobial peptide of PSI10. The binding capability results showed that BMP2-MP and PSI10-containing HABD were firmly bound to the surface of HA scaffolds. After BMP2-MP and PSI10 were bound to the scaffold surface, no negative effect was observed on cell proliferation and adhesion. The gene expression and protein translation levels of type I collagen (COL-I), osteocalcin (OCN) and Runx2 have been significantly improved in the BMP2-MP/HABP group. The level of alkaline phosphatase significantly increased in the BMP2-MP/HABP group. The inhibition zone test against Staphylococcus aureus and Escherichia coli BL21 prove that the PSI10/[email protected] scaffold has strong antibacterial ability than another group. These findings suggest that 3D-printed HA scaffolds with efficient osteoinduction and antimicrobial activity represent a promising biomaterial for bone defect reconstruction.
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http://dx.doi.org/10.1186/s13036-021-00273-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353754PMC
August 2021

Enhanced Bandwidth Broadening of Infrared Reflector Based on Polymer Stabilized Cholesteric Liquid Crystals with Poly(N-vinylcarbazole) Used as Alignment Layer.

Polymers (Basel) 2021 Jul 8;13(14). Epub 2021 Jul 8.

SCNU-TUE Joint Lab of Device Integrated Responsive Materials (DIRM), National Center for International Research on Green Optoelectronics, South China Normal University, Guangzhou 510006, China.

Alignment layer plays a critical role on liquid crystal (LC) conformation for most LC devices. Normally, polyimide (PI) or polyvinyl alcohol (PVA), characterized by their outstanding thermal and electrical properties, have been widely applied as the alignment layer to align LC molecules. Here, we used a semi-conductive material poly(N-vinylcarbazole) (PVK) as the alignment layer to fabricate the cholesteric liquid crystal (CLC) device and the polymer-stabilized cholesteric liquid crystals (PSCLC)-based infrared (IR) reflectors. In the presence of ultraviolet (UV) irradiation, there are hole-electron pairs generated in the PVK layer, which neutralizes the impurity electrons in the LC-PVK junction, resulting in the reduction in the built-in electric field in the LC device. Therefore, the operational voltage of the CLC device switching from cholesteric texture to focal conic texture decreases from 45 V to 30 V. For the PSCLC-based IR reflectors with the PVK alignment layer, at the same applied electric field, the reflection bandwidth is enhanced from 647 to 821 nm, ranging from 685 to 1506 nm in the IR region, which makes it attractive for saving energy as a smart window.
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http://dx.doi.org/10.3390/polym13142238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8309408PMC
July 2021

Improved Point-Line Feature Based Visual SLAM Method for Complex Environments.

Sensors (Basel) 2021 Jul 5;21(13). Epub 2021 Jul 5.

College of Communication and Information Engineering, Chongqing University of Posts and Telecommunications, Chongqing 400065, China.

Traditional visual simultaneous localization and mapping (SLAM) systems rely on point features to estimate camera trajectories. However, feature-based systems are usually not robust in complex environments such as weak textures or obvious brightness changes. To solve this problem, we used more environmental structure information by introducing line segments features and designed a monocular visual SLAM system. This system combines points and line segments to effectively make up for the shortcomings of traditional positioning based only on point features. First, ORB algorithm based on local adaptive threshold was proposed. Subsequently, we not only optimized the extracted line features, but also added a screening step before the traditional descriptor matching to combine the point features matching results with the line features matching. Finally, the weighting idea was introduced. When constructing the optimized cost function, we allocated weights reasonably according to the richness and dispersion of features. Our evaluation on publicly available datasets demonstrated that the improved point-line feature method is competitive with the state-of-the-art methods. In addition, the trajectory graph significantly reduced drift and loss, which proves that our system increases the robustness of SLAM.
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http://dx.doi.org/10.3390/s21134604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272192PMC
July 2021

Depletion of acetate-producing bacteria from the gut microbiota facilitates cognitive impairment through the gut-brain neural mechanism in diabetic mice.

Microbiome 2021 06 25;9(1):145. Epub 2021 Jun 25.

Institute of Metabonomics & Medical NMR, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China.

Background: Modification of the gut microbiota has been reported to reduce the incidence of type 1 diabetes mellitus (T1D). We hypothesized that the gut microbiota shifts might also have an effect on cognitive functions in T1D. Herein we used a non-absorbable antibiotic vancomycin to modify the gut microbiota in streptozotocin (STZ)-induced T1D mice and studied the impact of microbial changes on cognitive performances in T1D mice and its potential gut-brain neural mechanism.

Results: We found that vancomycin exposure disrupted the gut microbiome, altered host metabolic phenotypes, and facilitated cognitive impairment in T1D mice. Long-term acetate deficiency due to depletion of acetate-producing bacteria resulted in the reduction of synaptophysin (SYP) in the hippocampus as well as learning and memory impairments. Exogenous acetate supplement or fecal microbiota transplant recovered hippocampal SYP level in vancomycin-treated T1D mice, and this effect was attenuated by vagal inhibition or vagotomy.

Conclusions: Our results demonstrate the protective role of microbiota metabolite acetate in cognitive functions and suggest long-term acetate deficiency as a risk factor of cognitive decline. Video Abstract.
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http://dx.doi.org/10.1186/s40168-021-01088-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8235853PMC
June 2021

Which methods are the most effective in enabling novice users to participate in ontology creation? A usability study.

Database (Oxford) 2021 06;2021

Department of Biology, University of Ottawa, 30 Marie Curie Road, Ottawa, ON K1N 6N5, Canada.

Producing findable, accessible, interoperable and reusable (FAIR) data cannot be accomplished solely by data curators in all disciplines. In biology, we have shown that phenotypic data curation is not only costly, but it is burdened with inter-curator variation. We intend to propose a software platform that would enable all data producers, including authors of scientific publications, to produce ontologized data at the time of publication. Working toward this goal, we need to identify ontology construction methods that are preferred by end users. Here, we employ two usability studies to evaluate effectiveness, efficiency and user satisfaction with a set of four methods that allow an end user to add terms and their relations to an ontology. Thirty-three participants took part in a controlled experiment where they evaluated the four methods (Quick Form, Wizard, WebProtégé and Wikidata) after watching demonstration videos and completing a hands-on task. Another think-aloud study was conducted with three professional botanists. The efficiency effectiveness and user confidence in the methods are clearly revealed through statistical and content analyses of participants' comments. Quick Form, Wizard and WebProtégé offer distinct strengths that would benefit our author-driven FAIR data generation system. Features preferred by the participants will guide the design of future iterations.
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http://dx.doi.org/10.1093/database/baab035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218699PMC
June 2021

Levels of Fibrin Degradation Products at Admission With Acute Ischemic Stroke Correlate With the NIH Stroke Scale Score 1 h After Intravenous Thrombolysis.

Front Neurol 2021 28;12:651867. Epub 2021 May 28.

Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Fibrin degradation products (FDPs) are fragments released by the plasmin-mediated degradation of fibrinogen or fibrin. Whether plasma levels of these fragments can predict the thrombolytic effect of recombinant tissue plasminogen activator (r-tPA) remains unknown. We performed a hospital-based study of patients with acute ischemic stroke (AIS) to explore the relationship between FDP levels at admission and the NIH Stroke Scale (NIHSS) score 1 h after thrombolysis treatment. In this retrospective, single-center study, the data of all patients with AIS who received r-tPA treatment at Beijing Tiantan Hospital from January 2019 to October 2020 were collected and analyzed. Demographic and clinical data, including laboratory examinations, were also analyzed. A total of 339 patients with AIS were included in this study. Of these, 151 showed favorable effects of r-tPA, and 188 showed unsatisfactory effects at 1 h after thrombolysis. Overall, we found an inverse relationship between the FDPs levels at admission and the NIHSS score. A significant difference was observed when using the interquartile range of the FDPs levels (1.31 μg/mL) as a cutoff value ( = 0.003, odds ratio [OR] = 1.95, 95% confidence interval [CI]: 1.26-3.01), even after adjusting for confounding factors ( = 0.003, OR = 2.23, 95% CI: 1.31-3.77). In addition, significant associations were observed in the tertile (T3) and quartile (Q3, Q4) FDP levels when compared with T1 or Q1. A nomogram was also employed to create a model to predict an unsatisfactory effect of r-tPA. We found that FDP levels, white blood cell count, age, D-dimer level, and body mass index could influence the thrombolytic effect of r-tPA. In conclusion, the present study demonstrated that the levels of FDPs at admission can be used as a prognostic factor to predict the curative effect of r-tPA.
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http://dx.doi.org/10.3389/fneur.2021.651867DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194400PMC
May 2021

Fatty Acid Stable Carbon Isotope Ratios Combined with Oxidation Kinetics for Characterization and Authentication of Walnut Oils.

J Agric Food Chem 2021 Jun 8;69(23):6701-6709. Epub 2021 Jun 8.

Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.

Walnut oil is vulnerable to oxidation due to its high content of polyunsaturated fatty acids and adulteration due to its high price. This study investigated 12 typical walnut oils from six main walnut-producing areas in China, using differential scanning calorimetry (DSC), Rancimat test, gas chromatography (GC), elemental analyzer-isotope ratio mass spectrometry (EA-IRMS) and GC-IRMS combined with oxidation kinetic analysis, Pearson correlation analysis, and principal component analysis (PCA). The melting and crystallization profiles using DSC indicated that walnut oils with a relatively high crystal onset temperature tended to be more stable against oxidation. Oleic acid was found to be the most characteristic fatty acid in walnut oil, with a content ranging from 13.84 to 35.08%. Two walnut oils with the highest oleic acid contents of 35.08 and 32.78% had the highest activation energies in nonisothermal DSC. Their predicted shelf lives based on the Rancimat test were 3.5-4.0 times longer than that of the oil with the highest α-linolenic acid at 4 °C and 3.1-3.5 times longer at 25 °C. The δC values of walnut oils were determined by EA-IRMS, and the δC values of fatty acids were determined by GC-IRMS. Fatty acid stable carbon isotope ratios combined with PCA were successfully applied to intuitively discriminate different walnut oils. The results suggested that fatty acid δC values determined by IRMS combined with chemometrics and lipid compositions are promising as a powerful means of vegetable oil authentication and discrimination.
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http://dx.doi.org/10.1021/acs.jafc.1c01843DOI Listing
June 2021

A ratiometric electrochemical sensor for selectively monitoring monoamine oxidase A in the live brain.

Chem Commun (Camb) 2021 Jul 8;57(53):6487-6490. Epub 2021 Jun 8.

School of Chemistry and Molecular Engineering, Shanghai Key Laboratory of Green Chemistry and Chemical Processes, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.

Herein, an electrochemical method for selectively sensing and accurately quantifying monoamine oxidase A (MAO-A) in the cortex and thalamus of a live mouse brain was reported. Using this tool, it was found that MAO-A increased Ca entry into neurons via the TPRM2 channel in the live mouse brain of an AD model.
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http://dx.doi.org/10.1039/d1cc00787dDOI Listing
July 2021

The aryl hydrocarbon receptor activates ceramide biosynthesis in mice contributing to hepatic lipogenesis.

Toxicology 2021 06 9;458:152831. Epub 2021 Jun 9.

Center for Molecular Toxicology and Carcinogenesis, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, 16802, USA. Electronic address:

Aryl hydrocarbon receptor (AHR) activation via 2,3,7,8-tetrachlorodibenzofuran (TCDF) induces the accumulation of hepatic lipids. Here we report that AHR activation by TCDF (24  μg/kg body weight given orally for five days) induced significant elevation of hepatic lipids including ceramides in mice, was associated with increased expression of key ceramide biosynthetic genes, and increased activity of their respective enzymes. Results from chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assay (EMSA) and cell-based reporter luciferase assays indicated that AHR directly activated the serine palmitoyltransferase long chain base subunit 2 (Sptlc2, encodes serine palmitoyltransferase 2 (SPT2)) gene whose product catalyzes the initial rate-limiting step in de novo sphingolipid biosynthesis. Hepatic ceramide accumulation was further confirmed by mass spectrometry-based lipidomics. Taken together, our results revealed that AHR activation results in the up-regulation of Sptlc2, leading to ceramide accumulation, thus promoting lipogenesis, which can induce hepatic lipid accumulation.
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http://dx.doi.org/10.1016/j.tox.2021.152831DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327397PMC
June 2021

Microbiome analysis combined with targeted metabolomics reveal immunological anti-tumor activity of icariside I in a melanoma mouse model.

Biomed Pharmacother 2021 Aug 2;140:111542. Epub 2021 Jun 2.

Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy of Precision Measurement Science and Technology, CAS, Wuhan 430071, China; Engineering Research Academy of High Value Utilization of Green Plants, Meizhou 514021, China; Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address:

Recent studies report that the gut microbiome can enhance systemic and antitumor immunity by modulating responses to antibody immunotherapy in melanoma patients. In this study, we found that icariside I, a novel anti-cancer agent isolated from Epimedium, significantly inhibited B16F10 melanoma growth in vivo through regulation of gut microbiota and host immunity. Oral administration of icariside I improved the microbiota community structure with marked restoration of Lactobacillus spp. and Bifidobacterium spp. abundance in the cecal contents of tumor-bearing mice. We also found that icariside I improves the levels of microbiota-derived metabolites such as short-chain fatty acids (SCFAs) and indole derivatives, consequently promoting repair of the intestinal barrier and reducing systemic inflammation of tumor-bearing mice. Icariside I exhibited strong immunological anti-tumor activity, directly manifested by up-regulation of multiple lymphocyte subsets including CD4+ and CD8+ T cells or NK and NKT cells in peripheral blood of tumor-bearing mice. Collectively, these results suggest that icariside I, via its microbiome remodeling and host immune regulation properties, may be developed as an anticancer drug.
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http://dx.doi.org/10.1016/j.biopha.2021.111542DOI Listing
August 2021

Unexpected Role of Achiral Glycine in Determining the Suprastructural Handedness of Peptide Nanofibrils.

ACS Nano 2021 06 28;15(6):10328-10341. Epub 2021 May 28.

State Key Laboratory of Heavy Oil Processing and School of Materials Science and Engineering, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao 266580, China.

Helical supramolecular architectures play important structural and functional roles in biological systems. Although their occurrence is widely perceived to correlate to fundamental chiral units including l-amino acids and d-sugars, the detailed relationship between molecular and supramolecular handedness is still unclear. At the same time, although achiral units are practically always in close proximity to chiral ones by covalent linkage along a polymeric chain, their effect on supramolecular handedness has received relatively less attention. Here, we designed a set of short amphiphilic peptides, in which an achiral glycine residue was incorporated at the interface between the hydrophobic and hydrophilic segments. We observed that glycine incorporation caused dramatic variations in suprastructural handedness in self-assembled peptide nanofibrils, and the effect of the hydrophilic charged residue at the C-terminus on supramolecular handedness was demolished, leading to chiral truncation. Furthermore, molecular dynamics simulations and quantum chemistry calculations revealed that the unanticipated role of the glycine residue in regulating supramolecular handedness originated from its effect on the conformational preference of single β-strands. Importantly, reduced density gradient analyses on single β-strands indicated that, due to the lack of a side chain in glycine, intricate noncovalent interactions were produced among the neighboring amino acid side chains of the incorporated glycine and its local backbone, resulting in diverse β-strand conformations.
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http://dx.doi.org/10.1021/acsnano.1c02547DOI Listing
June 2021

Living-System-Driven Evolution of Self-Assembled-Peptide Probes: For Boosting Glioma Theranostics.

Anal Chem 2021 06 27;93(22):8035-8044. Epub 2021 May 27.

Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Institute of Engineering Medicine, Beijing Institute of Technology, Beijing 100081, PR China.

The primary principle for new molecular evolution is from nature, mimicking nature, and beyond nature, since it is extremely important for the artificial molecules to keep their structure and function in the natural system. It is especially true for the self-assembled supramolecular construction in complicated living bodies. Herein, we put forward a directed evolution strategy consisting of high-content screening from the living system and artificial modification in order to find "totipotential peptides" in a precise way. Progressive dimension reduction of the capability and precise anchoring of the target were realized. Through the living system evolution, we obtain a glioma-targeting and living system-induced self-assembled leading compound CCP. Through the artificial evolution, CCP was further stapled and was hydrophobically modified as SCCP, which demonstrated stability and NIR-II emission characteristics. SCCP could realize high-resolution molecular imaging and therapy simultaneously. We envision that the strategy and its applications provide a new method for molecular discovery and improve the performance of peptide nano-self-assemblies for diagnostics and therapy.
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http://dx.doi.org/10.1021/acs.analchem.1c01151DOI Listing
June 2021

Application of micro-nanostructured magnetite in separating tetrabromobisphenol A and hexabromocyclododecane from environmental water by magnetic solid phase extraction.

PLoS One 2021 3;16(5):e0251021. Epub 2021 May 3.

Jiangsu Provincial Key Laboratory of Environmental Engineering, Jiangsu Provincial Academy of Environmental Science, Nanjing, Jiangsu, China.

Two typical brominated flame retardants (BFRs), namely, tetrabromobisphenol A (TBBPA) and hexabromocyclododecane (HBCD), were persistent organic pollutants widely detected in various environmental media. This study aimed to successfully synthesize micro-nano-structured magnetite particles (MNMPs) with surface modification by citric acid molecules. The synthesized composites served as an adsorbent for extracting TBBPA and HBCD from environmental water samples followed by gas chromatography-mass spectrometry analysis. The obtained MNMPs were characterized in terms of crystal structure, morphology, size distribution, hydrophobic and hydrophilic performance and magnetism. The results indicated that the MNMPs exhibited high surface area, good dispersibility, and strong magnetic responsiveness for separation. The parameters affecting the extraction efficiency were optimized, including sample pH, amount of sorbents, extraction time and desorption conditions. Under the optimum conditions, the recovery was 83.5 and 107.1%, limit of detection was 0.13 and 0.35μg/mL (S/N = 3), and limit of quantification was 0.37 and 0.59 μg/mL (S/N = 10) for TBBPA and HBCD respectively. The relative standard deviations obtained using the proposed method were less than 8.7%, indicating that the MNMP magnetic solid-phase extraction method had advantages of simplicity, good sensitivity and high efficiency for the extraction of the two BFRs from environmental water.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0251021PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092778PMC
May 2021

The GW2-WG1-OsbZIP47 pathway controls grain size and weight in rice.

Mol Plant 2021 Aug 27;14(8):1266-1280. Epub 2021 Apr 27.

State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Centre for Excellence in Molecular Plant Biology, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100039, China. Electronic address:

Regulation of seed size is a key strategy for improving crop yield and is also a basic biological question. However, the molecular mechanisms by which plants determine their seed size remain elusive. Here, we report that the GW2-WG1-OsbZIP47 regulatory module controls grain width and weight in rice. WG1, which encodes a glutaredoxin protein, promotes grain growth by increasing cell proliferation. Interestingly, WG1 interacts with the transcription factor OsbZIP47 and represses its transcriptional activity by associating with the transcriptional co-repressor ASP1, indicating that WG1 may act as an adaptor protein to recruit the transcriptional co-repressor. In contrary, OsbZIP47 restricts grain growth by decreasing cell proliferation. Further studies reveal that the E3 ubiquitin ligase GW2 ubiquitinates WG1 and targets it for degradation. Genetic analyses confirm that GW2, WG1, and OsbZIP47 function in a common pathway to control grain growth. Taken together, our findings reveal a genetic and molecular framework for the control of grain size and weight by the GW2-WG1-OsbZIP47 regulatory module, providing new targets for improving seed size and weight in crops.
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http://dx.doi.org/10.1016/j.molp.2021.04.011DOI Listing
August 2021

Defect Induced Polarization Loss in Multi-Shelled Spinel Hollow Spheres for Electromagnetic Wave Absorption Application.

Adv Sci (Weinh) 2021 Apr 8;8(8):2004640. Epub 2021 Feb 8.

MOE Key Laboratory of Material Physics and Chemistry under Extraordinary School of Physical Science and Technology Northwestern Polytechnical University Xi'an 710072 China.

Defect engineering is an effective approach to manipulate electromagnetic (EM) parameters and enhance absorption ability, but defect induced dielectric loss dominant mechanism has not been completely clarified. Here the defect induced dielectric loss dominant mechanism in virtue of multi-shelled spinel hollow sphere for the first time is demonstrated. The unique but identical morphology design as well as suitable composition modulation for serial spinels can exclude the disturbance of EM wave dissipation from dipolar/interfacial polarization and conduction loss. In temperature-regulated defect in NiCoO serial materials, two kinds of defects, defect in spinel structure and oxygen vacancy are detected. Defect in spinel structure played more profound role on determining materials' EM wave dissipation than that of oxygen vacancy. When evaluated serial Co-based materials as absorbers, defect induced polarization loss is responsible for the superior absorption performance of NiCoO-based material due to its more defect sites in spinel structure. It is discovered that electron spin resonance test may be adopted as a novel approach to directly probe EM wave absorption capacities of materials. This work not only provides a strategy to prepare lightweight, efficient EM wave absorber but also illustrates the importance of defect engineering on regulation of materials' dielectric loss capacity.
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http://dx.doi.org/10.1002/advs.202004640DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061380PMC
April 2021

Multi-stage responsive peptide nanosensor: Anchoring EMT and mitochondria with enhanced fluorescence and boosting tumor apoptosis.

Biosens Bioelectron 2021 Jul 19;184:113235. Epub 2021 Apr 19.

Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address:

Epithelial-mesenchymal transition (EMT) is closely related to tumor metastasis and invasion. Thereinto, mesenchymal tumor mitochondria are the critical target for tumor inhibition. Therefore, real-time in vivo monitoring of EMT as well as inhibiting mesenchymal tumor mitochondria is of great diagnosis and therapy significance. Herein, we construct a multi-stage recognition and morphological transformable self-assembly-peptide nano biosensor DRP which can response the EMT marker and specifically damage the mesenchymal tumor cell in vivo. This nano-molar-affinity sensor is designed and screened with sensitive peptides containing a molecular switching which could be specifically triggered by the receptor to achieve the vesicle-to-fibril transformation in living system with enhanced fluorescent signal. DRP nanosensor could target the tumor lesion in circulatory system, recognize mesenchymal tumor marker DDR2 (Discoidin domain receptor 2) in cellular level and specifically achieve mitochondria in subcellular level as well as damaged mitochondria which could be applied as a in vivo theranostic platform.
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http://dx.doi.org/10.1016/j.bios.2021.113235DOI Listing
July 2021

Elevated GAS2L3 Expression Correlates With Poor Prognosis in Patients With Glioma: A Study Based on Bioinformatics and Immunohistochemical Analysis.

Front Genet 2021 30;12:649270. Epub 2021 Mar 30.

Tianjin Key Laboratory of Cerebral Vascular and Neurodegenerative Diseases, Tianjin Neurosurgical Institute, Tianjin Huanhu Hospital, Tianjin, China.

Background: Growth arrest-specific 2 like 3 (GAS2L3) is a cytoskeleton-associated protein that interacts with actin filaments and tubulin. Abnormal GAS2L3 expression has been reported to be associated with carcinogenesis. However, the biological role of GAS2L3 in glioma remains to be determined.

Methods: The transcriptome level of GAS2L3 and its relationship with clinicopathological characteristics were analyzed among multiple public databases and clinical specimens. Bioinformatics analyses were conducted to explore biological functions and prognostic value of GAS2L3 in glioma.

Results: GAS2L3 was substantially expressed in glioma, and high GAS2L3 expression correlated with shorter overall survival time and poor clinical variables. Gene set enrichment analysis (GSEA), single-sample gene-set enrichment analysis, and CIBERSORT algorithm analyses showed that GAS2L3 expression was closely linked to immune-related pathways, inflammatory activities, and immune cell infiltration. Moreover, GAS2L3 was synergistic with T cell-inflamed gene signature, immune checkpoints, T-cell receptor diversities, and neoantigen numbers.

Conclusion: This study suggests that GAS2L3 is a prognostic biomarker for glioma, providing a reference for further study of the potential role of GAS2L3 in the immunomodulation of glioma.
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http://dx.doi.org/10.3389/fgene.2021.649270DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042292PMC
March 2021

Aptamers: The Powerful Molecular Tools for Virus Detection.

Chem Asian J 2021 Jun 1;16(11):1298-1306. Epub 2021 May 1.

State Key Laboratory of Medicinal Chemical biology, College of Life Sciences, Nankai University, Tianjin, 300350, P. R. China.

Aptamers are short single-stranded DNA or RNA oligonucleotides selected by the technique of systematic evolution of ligands by exponential enrichment (SELEX). Aptamers have been demonstrated to bind various targets from small-molecule to cells or even tissues in the way of antibodies. Thus, they are called chemical antibodies. We summarize and evaluate recent developments in aptamer-based sensors (for short aptasensors) for virus detection in this review. These aptasensors are mainly classified into optical and electronic aptasensors based on the type of transducer. Nowadays, the smartphone has become the most widely used mobile device with billions of users worldwide. Considering the ongoing COVID-19 outbreak, smartphone-based aptasensors for a portable and point-of-care test (POCT) of COVID-19 detection will be of great importance in the future.
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http://dx.doi.org/10.1002/asia.202100242DOI Listing
June 2021

Combined Effects of Fe(III)-Bearing Clay Minerals and Organic Ligands on U(VI) Bioreduction and U(IV) Speciation.

Environ Sci Technol 2021 05 6;55(9):5929-5938. Epub 2021 Apr 6.

Biosciences Division, Argonne National Laboratory, Lemont, Illinois 60439, United States.

Reduction of U(VI) to U(IV) drastically reduces its solubility and has been proposed as a method for remediation of uranium contamination. However, much is still unknown about the kinetics, mechanisms, and products of U(VI) bioreduction in complex systems. In this study, U(VI) bioreduction experiments were conducted with strain CN32 in the presence of clay minerals and two organic ligands: citrate and EDTA. In reactors with U and Fe(III)-clay minerals, the rate of U(VI) bioreduction was enhanced due to the presence of ligands, likely because soluble Fe- and Fe-ligand complexes served as electron shuttles. In the presence of citrate, bioreduced U(IV) formed a soluble U(IV)-citrate complex in experiments with either Fe-rich or Fe-poor clay mineral. In the presence of EDTA, U(IV) occurred as a soluble U(IV)-EDTA complex in Fe-poor montmorillonite experiments. However, U(IV) remained associated with the solid phase in Fe-rich nontronite experiments through the formation of a ternary U(IV)-EDTA-surface complex, as suggested by the EXAFS analysis. Our study indicates that organic ligands and Fe(III)-bearing clays can significantly affect the microbial reduction of U(VI) and the stability of the resulting U(IV) phase.
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http://dx.doi.org/10.1021/acs.est.0c08645DOI Listing
May 2021

Deep understanding of impedance matching and quarter wavelength theory in electromagnetic wave absorption.

J Colloid Interface Sci 2021 Aug 26;595:1-5. Epub 2021 Mar 26.

MOE Key Laboratory of Material Physics and Chemistry under Extraordinary, School of Physical Science and Technology, Northwestern Polytechnical University, Xi'an 710072, China. Electronic address:

It is of great significance for absorber designers to correctly understand impedance matching and its related calculation methods, because excellent impedance matching effect is an important prerequisite for absorber to consume electromagnetic wave (EMW). However, there are some problems in literatures, such as the lack of deep understanding of impedance matching and application conditions of Eq. (3) for the calculation of reflection loss (RL). Based on the transmission line theory of electronic circuit, this paper expounds the definition, symbol, property of various impedances and their influence on impedance matching effect. On the basis of simplifying the actual situation in the process of EMW absorption to the correct transmission line model, the original transmission line model of RL calculation formula and its limitation are pointed out. The doubts about the validity of the quarter wavelength theory in literatures are explained from three aspects: the reduction of EMW length, the multiple reflection and the impedance transformation of absorber.
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http://dx.doi.org/10.1016/j.jcis.2021.03.132DOI Listing
August 2021

NMR-Based Metabolomics in Cancer Research.

Adv Exp Med Biol 2021 ;1280:201-218

State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, Key Laboratory of Magnetic Resonance in Biological Systems, National Center for Magnetic Resonance in Wuhan, Wuhan National Laboratory for Optoelectronics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, China.

Nuclear magnetic resonance (NMR) spectroscopy offers reproducible quantitative analysis and structural identification of metabolites in various complex biological samples, such as biofluids (plasma, serum, and urine), cells, tissue extracts, and even intact organs. Therefore, NMR-based metabolomics, a mainstream metabolomic platform, has been extensively applied in many research fields, including pharmacology, toxicology, pathophysiology, nutritional intervention, disease diagnosis/prognosis, and microbiology. In particular, NMR-based metabolomics has been successfully used for cancer research to investigate cancer metabolism and identify biomarker and therapeutic targets. This chapter highlights the innovations and challenges of NMR-based metabolomics platform and its applications in cancer research.
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http://dx.doi.org/10.1007/978-3-030-51652-9_14DOI Listing
April 2021

Nonenzymatic Electrochemical Sensor with Ratiometric Signal Output for Selective Determination of Superoxide Anion in Rat Brain.

Anal Chem 2021 04 23;93(13):5570-5576. Epub 2021 Mar 23.

School of Chemistry and Molecular Engineering, East China Normal University, Dongchuan Road 500, Shanghai 200241, P. R. China.

There is still an urgent need to develop reliable analytical methods of O in vivo for deeply elucidating the roles of O playing in the brain. Herein, a nonenzymatic electrochemical sensor with ratiometric signal output was developed for an in vivo analysis of O in the rat brain. Diphenylphosphonate-2-naphthol ester (ND) was designed and synthesized as a specific recognition molecule for the selective determination of O. An anodic peak ascribed to the oxidation of 2-naphthol was generated via the nucleophilic substitution between ND and O and was increased with the increasing concentration of O. Meanwhile, the inner reference of methylene blue (MB) was co-assembled at the electrode surface to enhance the determination accuracy of O. The anodic peak current ratio between 2-naphthol and MB exhibited a good linear relationship with the concentration of O from 2 to 200 μM. Because of the stable molecule character of ND and its specific reaction with O, the developed electrochemical sensor demonstrated excellent selectivity toward various potential interferences in the brain and good stability even after storage for 7 days. Accordingly, the present electrochemical sensor with high selectivity, high stability, and high accuracy was successfully exploited in monitoring the levels of O in the rat brain and that of the diabetic model followed by cerebral ischemia.
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http://dx.doi.org/10.1021/acs.analchem.1c00151DOI Listing
April 2021

Gut Microbiota and Its Metabolite Deoxycholic Acid Contribute to Sucralose Consumption-Induced Nonalcoholic Fatty Liver Disease.

J Agric Food Chem 2021 Apr 23;69(13):3982-3991. Epub 2021 Mar 23.

Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Innovation Academy for Precision Measurement Science and Technology, CAS, Wuhan 430071, China.

As important signal metabolites within enterohepatic circulation, bile acids (BAs) play a pivotal role during the occurrence and development of diet-induced nonalcoholic fatty liver disease (NAFLD). Here, we evaluated the functional effects of BAs and gut microbiota contributing to sucralose consumption-induced NAFLD of mice. The results showed that sucralose consumption significantly upregulated the abundance of intestinal genera and , which produced deoxycholic acid (DCA) accumulating in multiple biological matrixes including feces, serum, and liver of mice. Subsequently, elevated hepatic DCA, one of the endogenous antagonists of the farnesol X receptor (), inhibited hepatic gene expression including a small heterodimer partner () and leading to sucralose-induced NAFLD in mice. Dietary supplements with fructo-oligosaccharide or metformin markedly restored genera and abundance and the DCA level of sucralose-consuming mice, which eventually ameliorated NAFLD. These findings highlighted the effects of gut microbiota and its metabolite DCA on sucralose-induced NAFLD of mice.
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http://dx.doi.org/10.1021/acs.jafc.0c07467DOI Listing
April 2021

Fibromuscular dysplasia of mesenteric arteries: a rare cause of multiple bowel resections-a case report and literature review.

BMC Gastroenterol 2021 Mar 22;21(1):133. Epub 2021 Mar 22.

Department of Gastroenterology, The Seventh Medical Center, Chinese PLA General Hospital, No.5, Nanmengcang Hutong, Beijing, China.

Background: Fibromuscular dysplasia (FMD) is a type of unexplained nonatherosclerotic vascular disease that usually involves the renal and internal carotid arteries and rarely involves the mesenteric artery. Mesenteric artery FMD is difficult to distinguish from Crohn's disease (CD) and Behcet's disease (BD) solely based on symptoms. Patients with mesenteric artery FMD can present with an acute abdomen, but case reports of patients who have a long medical history and undergo multiple bowel resections are extremely rare.

Case Presentation: The patient was a 45-year-old woman with an 11-year history of intermittent lower abdominal pain and fever. At the age of 34 years, she underwent right hemicolectomy and appendectomy due to an acute abdomen. She suffered from oral ulcers between 34 and 36 years old. A clinical diagnosis of presumed CD was made by the age of 41, and she was treated with mesalazine; however, the effect was poor. At the age of 42, she came to our centre, and based on her atypical symptoms and examination results, we thought she had CD. Hence, she was treated with glucocorticoids for 3 years. However, when she was 45, due to steroid dependence, thalidomide tablets were added. Unfortunately, she suffered from another episode of intestinal obstruction. Therefore, she underwent enterectomy. The postoperative histopathological diagnosis was mesenteric artery FMD. She no longer underwent pharmacotherapy after the surgery. Although she did not have any of her previous symptoms and postoperative colonoscopy showed no signs of recurrence, splenomegaly and abnormal routine blood results were still present.

Conclusions: Patients with mesenteric artery FMD can present with an acute abdomen. In addition, the symptoms and endoscopic manifestations of mesenteric artery FMD may appear similar to CD and BD. Hence, it is difficult to make a clear clinical diagnosis and proceed with treatment. Mesenteric artery FMD often requires surgical pathology to confirm its diagnosis. For patients who suffer from this disorder, surgery may be the best choice to improve the patient's quality of life.
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http://dx.doi.org/10.1186/s12876-021-01702-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986496PMC
March 2021
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