Publications by authors named "Liliane Janete Grando"

23 Publications

  • Page 1 of 1

Cell-block as ancillary tool for the diagnosis of cystic and cyst-like jaw lesions.

Diagn Cytopathol 2020 Aug 29;48(8):717-723. Epub 2020 Feb 29.

Department of Pathology, Federal University of Santa Catarina (UFSC), Florianópolis, Brazil.

Background: The cell-block technique is a cytological diagnostic method that decreases cellular dispersion; however, its usefulness regarding intraosseous lesions is underexplored. Therefore, this study aimed to assess the diagnostic usefulness of cell-block for cystic and cyst-like jaw lesions.

Methods: Individuals with cystic or cyst-like jaw lesions who underwent aspiration biopsy were considered eligible. Aspiration of luminal content was prepared and processed by the cell-block technique. Cytological evaluations were blindly performed by two trained PhD students (in a single evaluation) and one oral and maxillofacial pathologist (OMP). Cohen's kappa statistic was used to measure inter-rater agreement.

Results: The sample was composed of 52 lesions, represented by 25 radicular cysts (RC), 17 odontogenic keratocysts (OKC), 5 idiopathic bone cavities (IBC), and 5 unicystic ameloblastomas (UA). The kappa coefficient of cell-block compared to histopathological diagnosis was 0.390 (95% confidence interval [95%CI], 0.195-0.585) for PhD students and 0.612 (95%CI, 0.433-0.791) for the OMP. The highest concordance rates between cell-block and histopathological diagnosis were observed for RC (PhD = 76.0%; OMP = 80.0%) and OKC (PhD = 58.8%; OMP = 76.5%). Conflicting results were found regarding IBC (PhD = 40.0%; OMP = 80.0%) and UA, that presented the overall lowest concordance rates (PhD = 20.0%; OMP = 40.0%).

Conclusion: The cell-block technique presented a high diagnostic usefulness for detecting RC and OKC and, if associated with clinical and radiographic characteristics, might be sufficient for final diagnosis of these diseases. Regarding IBC and UA, an analysis with a higher number of cases is recommended to determine the true usefulness of the cell-block as ancillary tool for the diagnosis of these lesions.
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http://dx.doi.org/10.1002/dc.24400DOI Listing
August 2020

Diagnostic Challenge and Clinical Management of Juvenile Mandibular Chronic Osteomyelitis.

Head Neck Pathol 2020 Sep 28;14(3):842-846. Epub 2019 Nov 28.

Department of Pathology, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

Juvenile mandibular chronic osteomyelitis (JMCO) is a rare, idiopathic disease of chronic bone inflammation without suppuration, sinus tract formation, or sequestration. As the name suggests, this condition predominately affects children. The few cases of JMCO reported in the literature describe different treatments, and thus a standard therapy protocol has not yet been established. The aim of this paper is to report a clinical case in a 9-year-old girl that was misdiagnosed and unsuccessfully treated for 1 year. After experiencing persistent symptoms, a correct diagnosis was subsequently rendered based on the physical and radiographic examination as well as successful treatment with non-steroidal anti-inflammatory drugs (NSAIDs). The patient received drug therapy followed by periods of remission over a 4 year follow-up period. Diagnosis and treatment of JMCO is a challenge given the rarity and nonspecific signs and symptoms of this condition. Treatment with NSAIDs and regular follow-up is a conservative option for these patients.
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http://dx.doi.org/10.1007/s12105-019-01087-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7413939PMC
September 2020

Subset of CD8+ and FOXP3 + T cells in lichen planus associated with chronic hepatitis C infection.

Oral Dis 2019 May 15;25(4):1100-1106. Epub 2019 Mar 15.

Oral and Maxillofacial Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil.

Objective: To verify whether there are differences between populations of CD8 + and FoxP3 + T cells in lesions of oral lichen planus associated with hepatitis C virus chronic infection (OLP-HCV) and lesions of idiopathic oral lichen planus (OLP-I).

Materials And Methods: A case-control study was performed using a convenience sample of 11 paraffin-embedded tissue blocks of OLP-HCV and 19 of OLP-I. Histological sections stained with haematoxylin and eosin were used to classify the intensity of inflammatory infiltrate. Immunohistochemistry was used to identify CD8 + and FoxP3 + T cells. The count of positive cells was compared between the two groups and correlated to clinical and demographic data (p < 0.05).

Results: There were no statistically significant differences in the distribution of CD8 + and FoxP3 + T cells regarding the inflammatory infiltrate in lesions of OLP-HCV and OLP-I. Atrophic/erosive lesions showed a higher relationship between counts of CD8/FoxP3 T cells per mm (p = 0.018) and counts of CD8 + T cells per mm (p = 0.034) in OLP-HCV group compared to OLP-I group.

Conclusion: Overall, no difference was found between cell populations in the lesions of OLP-HCV and OLP-I. However, atrophic/erosive lesions of OLP-HCV had a higher amount of CD8 + T cells and lower FoxP3 expression.
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http://dx.doi.org/10.1111/odi.13069DOI Listing
May 2019

Osteoradionecrosis of the jaws: case series treated with adjuvant low-level laser therapy and antimicrobial photodynamic therapy.

J Appl Oral Sci 2018 21;26:e20170172. Epub 2018 May 21.

Núcleo de Odontologia Hospitalar do Hospital Universitário, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil.

Background: Osteoradionecrosis of the jaw (ORNJ) is the most severe and complex sequel of head and neck radiotherapy (RT) because of the bone involved, it may cause pain, paresthesia, foul odor, fistulae with suppuration, need for extra oral communication and pathological fracture. We treated twenty lesions of ORNJ using low-level laser therapy (LLLT) and antimicrobial photodynamic therapy (aPDT). The objective of this study was to stimulate the affected area to homeostasis and to promote the healing of the oral mucosa.

Methods: We performed aPDT on the exposed bone, while LLLT was performed around the bone exposure (red spectrum) and on the affected jaw (infrared spectrum). Monitoring and clinical intervention occurred weekly or biweekly for 2 years.

Results: 100% of the sample presented clinical improvement, and 80% presented complete covering of the bone exposure by intact oral mucosa.

Conclusion: LLLT and aPDT showed positive results as an adjuvant therapy to treat ORNJ.
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http://dx.doi.org/10.1590/1678-7757-2017-0172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5953563PMC
May 2018

Incidence of reactive hyperplastic lesions in the oral cavity: a 10 year retrospective study in Santa Catarina, Brazil.

Braz J Otorhinolaryngol 2019 Jul - Aug;85(4):399-407. Epub 2018 Apr 17.

Universidade Federal de Santa Catarina (UFSC), Departamento de Patologia, Florianópolis, SC, Brazil.

Introduction: Reactive hyperplastic lesions develop in response to a chronic injury simulating an exuberant tissue repair response. They represent some of the most common oral lesions including inflammatory fibrous hyperplasia, oral pyogenic granuloma, giant cell fibroma, peripheral ossifying fibroma, and peripheral giant cell lesions.

Objective: The incidence of those lesions was investigated in an oral pathology service, and the clinical characteristics, associated etiological factors, concordance between the clinical and histopathological diagnostic was determined.

Methods: A total of 2400 patient records were screened from 2006 to 2016. Clinical features were recorded from biopsy reports and patients' files.

Results: A total of 534 cases of reactive hyperplastic lesions were retrieved and retrospectively studied, representing 22.25% of all diagnoses. The most frequent lesion was inflammatory fibrous hyperplasia (72.09%), followed by oral pyogenic granuloma (11.79%), giant cell fibroma (7.30%), peripheral ossifying fibroma (5.24%), and peripheral giant cell lesions (3.55%). Females were predominantly affected (74.19%), the gingiva and alveolar ridge were the predominant anatomical site (32.89%), and chronic traumatism was presented as the main etiological factor. The age widely ranges from the 1st decade of life to the 7th. Clinically, the reactive hyperplastic lesions consisted of small lesions (0.5-2cm) and shared a strong likeness in color to the oral mucosa. The concordance between the clinical and histopathological diagnostic was high (82.5%).

Conclusion: Reactive hyperplastic lesions had a high incidence among oral pathologies. The understanding of their clinical features helps to achieve a clearer clinical and etiological diagnosis, and the knowledge of factors related to their development. This may contribute to adequate treatment and positive prognosis.
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http://dx.doi.org/10.1016/j.bjorl.2018.03.006DOI Listing
September 2019

The use of tools to support oral lesion description in oral medicine referrals.

Braz Oral Res 2017 Nov 27;31:e93. Epub 2017 Nov 27.

Universidade Federal de Santa Catarina - UFSC, Department of Pathology, Clinic of Stomatology, Florianópolis, SC, Brazil.

Poor description of oral lesions jeopardize the prioritization of appointments in Oral Medicine. The present study investigated whether the use of support tools changes the quality of descriptions focusing on health care prioritization. Two oral lesions (A and B) were described by 64 dental students and 48 dentists using three methods: (a) without support tools (free); (b) using the oral examination form from the Specialties Manual in Oral Health/Brazilian Ministry of Health (SMOH form); and (c) using the OralDESC guideline. The descriptions were compared with a gold standard and percentage of agreement was analyzed by the Kruskal-Wallis and Dunn's tests (p<0.05). Descriptions with the OralDESC presented higher information quality. Considering items essential for prioritization, the OralDESC demonstrated better performance for lesion A; for lesion B, free descriptions and descriptions using the OralDESC were of higher quality than those using SMOH form. Therefore, the OralDESC offered greater support for the description of oral lesions for health care prioritization in Oral Medicine.
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http://dx.doi.org/10.1590/1807-3107BOR-2017.vol31.0093DOI Listing
November 2017

Salivary evaluation in radioactive I treated patients with thyroid carcinoma.

Acta Odontol Scand 2018 Mar 10;76(2):148-152. Epub 2017 Nov 10.

a Department of Pathology, Health Science Center , Federal University of Santa Catarina , Florianopolis , SC , Brazil.

Background And Objective: radioiodine treatment (I) used to treat thyroid carcinomas produces side effects (sialadenitis, xerostomia, dysphagia and caries susceptibility) reflecting in a poor patient quality of life. This study aimed to evaluate the effect of I on salivary function and possible oral impairment.

Material And Methods: Thirty-seven patients undergoing I were submitted to oral examination, answer questions regarding xerostomia/hyposalivation and collect saliva at three moments (M1: 30-45 days before I, M2: 1-2 days after I and M3: 7-10 days after treatment). Saliva was assayed for flow rate and calcium/phosphate concentrations.

Results And Conclusions: significant difference in calcium/phosphate concentration was shown between M1 and M2, with evident decrease at M2. Flow rate reduced right after treatment with 41% of patients returning to previous rate at M3 (no statistical difference). A higher number of patients related xerostomia and difficulty in swallowing food at M2. The results showed that xerostomia/hyposalivation, dysphagia and calcium/phosphate concentration decrease may be considered early radioiodine side effects.
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http://dx.doi.org/10.1080/00016357.2017.1399214DOI Listing
March 2018

Aspiration and cytological evaluation of idiopathic bone cavities of the jaw.

Tissue Cell 2017 Jun 20;49(3):435-439. Epub 2017 Mar 20.

Department of Pathology, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

The idiopathic bone cavity (IBC) is an intraosseous pseudocyst devoid of epithelial lining. Clinically, IBCs of the jaw are asymptomatic and normally found in routine radiographic exams. Although the literature regarding the content of IBCs is controversial, the final diagnosis is usually aided by the discovery of an empty cavity upon surgical exploration. The aim of this study was to perform cytological and histological analysis of IBC contents. Cytological analysis of nine cases of IBC was performed after puncture and processed by the cell block technique. Histological analysis was performed in six cases in which it was possible to collect enough material by curettage of bone walls. Remarkably, cell block analysis revealed the presence of fibrin, often arranged as a net; erythrocytes; and inflammatory cells, with a predominance of lymphocytes as well as some macrophages and neutrophils. Histological analysis showed the presence of scant connective tissue, bone trabeculae, hemorrhagic foci, and hemosiderin. Only two cases presented scattered multinucleated giant cells. Cytological evaluation of IBC content by the cell block technique might represent a useful diagnostic tool, especially in cases in which there is no available material for curettage in the cavity.
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http://dx.doi.org/10.1016/j.tice.2017.03.005DOI Listing
June 2017

Proliferative verrucous leukoplakia: diagnosis, management and current advances.

Braz J Otorhinolaryngol 2017 Sep - Oct;83(5):585-593. Epub 2017 Jan 24.

Universidade Federal de Santa Catarina (UFSC), Departamento de Patologia, Florianopolis, SC, Brazil. Electronic address:

Introduction: Proliferative verrucous leukoplakia is a multifocal and progressive lesion of the oral mucosa, with unknown etiology, and commonly resistant to all therapy attempts with frequent recurrences. It is characterized by a high rate of oral squamous cell carcinoma and verrucou carcinoma transformations.

Objective: To analyze the studies about Proliferative verrucous leukoplakia and develop a concise update.

Methods: A Pubmed search identifying studies (laboratory research, case series and reviews of literature) that examined patients with Proliferative verrucous leukoplakia was realized.

Results: There are not enough studies about Proliferative verrucous leukoplakia in the literature. The few found studies not present a consensus about its etiology and diagnosis criteria. Although several treatment strategies have been proposed, most of them still show a high recurrence rate.

Conclusion: More research about Proliferative verrucous leukoplakia is necessary to understand and treat this disease.
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http://dx.doi.org/10.1016/j.bjorl.2016.12.005DOI Listing
December 2017

Medication-Related Osteonecrosis of Jaws: A Low-Level Laser Therapy and Antimicrobial Photodynamic Therapy Case Approach.

Case Rep Dent 2016 7;2016:6267406. Epub 2016 Sep 7.

Department of Pathology, Ambulatory of Stomatology, University Hospital, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

Medication-related osteonecrosis of the jaws (MRONJ) can be considered an inability of the alveolar bone to respond to an injury, which frequently leads to severe local and systemic complications. Once the problem is installed, dentist must use all therapeutic approaches recommended. This manuscript reports a successful management of MRONJ handled with antibiotics, conservative debridement, low-level laser therapy (LLLT), and photodynamic therapy (PDT) up to 12 months. As healing of MRONJ may be very slow, combined therapeutic approaches are required. Besides the recommended conventional treatment protocol, LLLT and PDT are important tools to contribute to healing and improvement of patient's quality of life.
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http://dx.doi.org/10.1155/2016/6267406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030412PMC
September 2016

Oral health related to quality of life in patients with stomatological diseases.

Stomatologija 2015 ;17(2):48-53

Campus Universitário João Davi Ferreira Lima, Bairro Trindade 88040-970, Florianópolis, SC, Brazil.

Aim: To describe the oral health-related quality of life (OHR-QoL) in patients with stomatological diseases.

Methods: All new patients seen in the Stomatology Clinic, from August 2008 to July 2009 were selected. OHR-QoL was measured using OHIP-14, in face-to-face interviews. The stomatological diseases were classified into groups according to their origin. OHIP-14 data were used to calculate two variables: prevalence and severity.

Results: The questionnaire was completed by 113 subjects, with a mean of 53.77 years; 63.7% women; 38% of the subjects reported one or more OHR-QoL impacts "fairly often" or "very often" in the last 6 months. The overall result of OHIP-14 index showed a mean of 14.35 (+/-12.01). There was no statistical significance between the stomatological disease groups and the prevalence scores (p=0.25) and mean severity scores (p=0.57).

Conclusions: It seems that bad oral conditions affected quality of life of these patients, especially physical pain and psychological discomfort.
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October 2017

A Rare Case of Mucoepidermoid Carcinoma ex Pleomorphic Adenoma arising in Minor Salivary Gland: Histopathological and Immunohistochemical Analysis.

J Contemp Dent Pract 2015 07 1;16(7):603-6. Epub 2015 Jul 1.

Department of Pathology, Health Sciences Center, Federal University of Santa Catarina, Santa Catarina, Brazil.

Mucoepidermoid carcinoma ex pleomorphic adenoma (MCxPA) is a rare salivary gland tumor predominantly found in major salivary glands. A case of MCxPA involving the soft tissue and bone of the retromolar region of a 26-year-old man is presented. The histopathological features revealed a neoplasm with predominance of pleomorphic adenoma (PA) elements, and presence of mucoepidermoid carcinoma malignant epithelial cells in several areas. Histochemical and immunohistochemical studies were positive for periodic acid Schiff, alcian blue, cytokeratins 7, 13, 14, and 19, Bcl-2, c-erbB-2, FGF-2 and maspin in the malignant areas. The patient underwent a partial resection of the left side of the mandible with neck dissection and MCxPA diagnosis was confirmed.
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http://dx.doi.org/10.5005/jp-journals-10024-1728DOI Listing
July 2015

Impact of leukemia and lymphoma chemotherapy on oral cavity and quality of life.

Spec Care Dentist 2015 Sep 12;35(5):236-242. Epub 2015 May 12.

Hematology Service, University Hospital/Federal University of Santa Catarina, Brazil.

This study aimed to understand how patients perceive their oral health and the resulting oral manifestations of antineoplastic chemotherapy, as well as to analyze the impact of these alterations on oral health-related quality of life. A total of 80 patients undergoing treatment participated in this study. A questionnaire was applied using the Oral Health Impact Profile (OHIP-14) index and open interviews. Items with the highest impact prevalence included "worsened taste of food sensation" (35.00%), "discomfort in eating food" (20.00%), and "feeling stressed" (17.50%). The outpatients showed the highest prevalence scores, whereas the inpatients presented higher quality of life impact severity. The ways in which the patients perceived how their oral alteration affected their quality of life were distinct and subjective. It is important that dentists act together with a multiprofessional team developing strategies to alleviate the impact of the disease and chemotherapy on oral cavity and patients' quality of life.
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http://dx.doi.org/10.1111/scd.12113DOI Listing
September 2015

Dental treatment in patients with leukemia.

J Oncol 2015 15;2015:571739. Epub 2015 Feb 15.

Integrated Multidisciplinary Health, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil.

Dental treatment of patients with leukemia should be planned on the basis of antineoplastic therapy which can be chemotherapy with or without radiotherapy and bone marrow transplantation. Many are the oral manifestations presented by these patients, arising from leukemia and/or treatment. In addition, performing dental procedures at different stages of treatment (before, during, or after) must follow certain protocols in relation to the haematological indices of patients, aimed at maintaining health and contributing to the effectiveness of the results of antineoplastic therapy. Through a literature review, the purpose of this study was to report the hematological abnormalities present in patients with leukemia, trying to correlate them with the feasibility of dental treatment at different stages of the disease. It is concluded in this paper that dental treatment in relation to haematological indices presented by patients with leukemia must follow certain protocols, mainly related to neutrophil and platelet counts, and the presence of the dentist in a multidisciplinary team is required for the health care of this patient.
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http://dx.doi.org/10.1155/2015/571739DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345074PMC
March 2015

Dentigerous cyst of inflammatory origin.

J Dent Child (Chic) 2014 May-Aug;81(2):112-6

Department of Pathology, São Thiago University Hospital, at the Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.

There is an association between persistent, prolonged inflammation of a primary tooth and the development of an inflammatory dentigerous cyst involving the succedaneous tooth. The purpose of this case report is to describe the management of an inflammatory dentigerous cyst of the permanent maxillary left central incisor in a nine-year-old boy caused by a long-term inflammation/infection of its predecessor. The treatment consisted of conservative decompression, which allowed for rapid healing and the eruption of the permanent tooth. The patient was followed up with periodic clinical and radiographic evaluations for several years.
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May 2015

Cell block technique as an additional tool in the diagnosis of ameloblastoma.

Braz Oral Res 2014 26;28. Epub 2014 Aug 26.

Department of Pathology, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.

The objective of this study was to evaluate the cytological content of ameloblastomas of the jaw. Nine cases of ameloblastoma were punctured, and the intralesional material was processed using the cell block technique. After centrifugation, the pellet obtained from the punctured material was fixed in formaldehyde and routinely processed to inclusion in paraffin. The obtained sections were stained with haematoxylin and eosin (H&E). Immunohistochemical reactions against anti-pan-cytokeratin (AE1/AE3) were performed to measure the presence of epithelial cells. Cytological analyses of the obtained slides revealed the presence of epithelial cells (as evidenced by AE1/AE3 labelling) and acellular amorphous eosinophilic materials. These cytological findings, in light of clinical and imaging data, can be helpful in the presumptive diagnosis of this disease entity by eliminating other possible diagnoses. Nonetheless further studies are needed in order to determine the nature of the acellular amorphous eosinophilic material and to ascertain the immunoprofile of epithelial cells.
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http://dx.doi.org/10.1590/1807-3107bor-2014.vol28.0044DOI Listing
November 2014

Utility of cell block in the cytological preoperative diagnosis of keratocystic odontogenic tumor.

Pathol Res Pract 2014 Apr 30;210(4):224-7. Epub 2013 Dec 30.

Department of Pathology, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.

In most cases involving jaw lesions, a biopsy and a histopathological analysis are necessary to establish the final diagnosis. However, biopsy may be a complex procedure at some maxillomandibular sites, and some systemic conditions could contraindicate the procedure. Thus, a search for new, less invasive techniques, which could eventually replace biopsy and simplify the diagnostic process, would benefit both professionals and patients. The aim of this study was to evaluate the cell block technique, prepared from the aspiration of luminal contents, in the preoperative diagnosis of keratocystic odontogenic tumors (KCOT). From 135 cases of lesions aspirated and processed by the cell block technique, we selected those containing keratin. In all cases selected, histological diagnosis was based on surgical biopsy. From 20 cases containing keratin in the cytological analyses, 19 were KCOTs and one was an orthokeratinized odontogenic cyst (OOC). In all KCOT cases, we observed the presence of parakeratin, even in those with intense inflammation. In the cytological analysis of the OOC, parakeratin was not observed. In conclusion, there is strong evidence that KCOT can be confidently diagnosed preoperatively by cytological analyses of lesions punctured and processed by the cell block technique.
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http://dx.doi.org/10.1016/j.prp.2013.12.006DOI Listing
April 2014

Evaluation of the cell block technique as an auxiliary method of diagnosing jawbone lesions.

Braz Oral Res 2012 Jul-Aug;26(4):355-9. Epub 2012 Jun 19.

Program in Dentistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

This study investigated the viability of the cell block technique as an auxiliary method of diagnosing jawbone lesions. Thirty-three clinically diagnosed jawbone lesions with a cystic appearance were subjected to aspiration. The aspirated material was processed by the cell block technique, and the lesions were biopsied and treated. Cytological findings (cell block) and histopathology analyses (gold standard) were compared by the chi-square test. There were associations between cysts and cholesterol crystal clefts, between keratocystic odontogenic tumors (KOT) and epithelial cells, and between KOT and parakeratin. The occurrence of cholesterol crystal clefts in cell block slides was correlated with cystic lesions, and the parakeratin presence was a KOT indicator. The cell block technique proved to be fast, easy-to-handle, and low-cost, making it an attractive auxiliary method for the preliminary diagnosis of jawbone lesions.
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http://dx.doi.org/10.1590/s1806-83242012005000011DOI Listing
January 2013

Cell block technique as a complementary method in the clinical diagnosis of cyst-like lesions of the jaw.

J Appl Oral Sci 2011 May-Jun;19(3):269-73

Department of Pathology, Health Science Center, Federal University of Santa Catarina, Florianópolis, SC, Brazil.

Objective: To evaluate the applicability of the cell block technique as a complementary method for presumptive diagnosis in the analysis of cyst-like aspirates from jaw lesions prior to histopathological diagnosis.

Material And Methods: The sample was made up of 17 cyst-like jaw lesions. After puncture, the aspirates were centrifuged, fixed in formalin, embedded in paraffin and processed. All lesions were biopsied and submitted to histopathological examination.

Results: In 9 cases, the cytopathological analysis using the cell block method showed a predominant presence of erythrocytes, inflammatory cells and few epithelial cells. In the other 8 cases, the cell block technique demonstrated the presence of parakeratin, whose histopathological analysis confirmed the occurrence of keratocystic odontogenic tumors (KOTs).

Conclusions: According to the studied cases, the cell block method was proven to be a simple, fast and low-cost technique to effectively differentiate KOTs from other lesions with similar clinical and radiographic features. The cell block technique comprises cellular preparations useful to determine a clinical diagnosis and help to develop a therapeutic plan for those lesions.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234341PMC
http://dx.doi.org/10.1590/s1678-77572011000300016DOI Listing
November 2011

Techniques for precancerous lesion diagnosis.

J Oncol 2011 13;2011:326094. Epub 2011 Jan 13.

Postgraduate Program of the Federal University of Santa Catarina, 88040-370 Florianopolis, SC, Brazil.

The development of the oral squamous cell carcinoma (OSCC) is a multistep process that requires the accumulation of multiple genetic alterations usually preceded by detectable mucosal changes, most often leukoplakias and erythroplakias. The clinical appearance of oral precancerous lesions and their degree of epithelium dysplasia suggests the malignization potential. Several techniques have been developed to improve the clinical and cytological diagnosis of oral precancerous lesions. The present paper reviews the main techniques used to improve premalignant lesion diagnosis.
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http://dx.doi.org/10.1155/2011/326094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3026985PMC
July 2011

A comparative study of the breathing pattern and amount of nasopharynx obstruction by the pharyngeal tonsil in HIV infected and non infected children.

Braz J Otorhinolaryngol 2007 Sep-Oct;73(5):583-91

Unisul.

Aim: the goal of the present investigation was to study the association between breathing pattern and pharyngeal tonsil size in 122 children (60 HIV infected and 62 without such infection).

Material And Methods: The children were analyzed as to their breathing pattern, nasal flow and pharyngeal tonsil obstruction seen in side cephalometric x-rays, by means of a computerized analysis.

Results: The pattern that most occurred in both groups was the mixed type. Most of the children presented oral or mixed type breathing and there was no association between the type of breathing and HIV presence (p=0.091). Nasal flow was mainly medium in both groups. Children without prior history of HIV infection had medium to large nasal flow and most of the HIV-infected children had medium nasal airflow. There was a positive association between nasal flow and HIV infection (p<0.0001). The average percentage of nasopharynx obstruction by the pharyngeal tonsil was high in both groups, and there was no statistically significance difference between them. Children from both groups had a moderate or large size of pharyngeal tonsil, and there was no association between tonsil size and HIV (p=0.21).
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http://dx.doi.org/10.1016/s1808-8694(15)30117-8DOI Listing
May 2008

Viral coinfection in the oral cavity of HIV-infected children: relation among HIV viral load, CD4+ T lymphocyte count and detection of EBV, CMV and HSV.

Braz Oral Res 2005 Jul-Sep;19(3):228-34. Epub 2005 Nov 21.

Department of Pathology, Federal University of Santa Catarina.

Viral coinfection in the oral cavity associated to HIV infection was evaluated in 180 children from birth to 13 years of age of both sexes. The oral examinations were performed at the Pediatric AIDS Outpatient Clinic, São Lucas Hospital and Clinic Hospital, both in Porto Alegre, Brazil and at the School of Dental Medicine, University Hospital Center, State University of New York at Stony Brook, USA. The aim of this study was to identify the presence of viral infections in the oral cavity. PCR technique was used to determine opportunistic viral infections caused by CMV, EBV, and HSV in mucosal swabs. A high frequency of viral infection was detected in the oral cavity of HIV-infected children determined by the PCR technique. HIV-infected children with viruses had a favorable CD4+ T lymphocyte count and unfavorable viral load.
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http://dx.doi.org/10.1590/s1806-83242005000300013DOI Listing
June 2006

[Oral manifestations, CD4+ T-lymphocytes count and viral load in Brazilian and North-American HIV-infected children].

Pesqui Odontol Bras 2002 Jan-Mar;16(1):18-25

Universidade Federal de Santa Catarina.

Oral manifestations related to HIV infection were evaluated in 184 children of both genders, aging 0-13 years. The oral exams were carried out at the Ambulatory of Pediatric AIDS, São Lucas Hospital and at the Clinics Hospital, both in Porto Alegre, RS, Brazil, and at the University Hospital and School of Dentistry, State University of New York, Stony Brook, NY, USA, from January 1999 to May 2000. A high prevalence of oral manifestations was observed both in Brazilian (72.73%) and American (53.66%) children. The most frequent manifestations were cervical lymphadenopathy, swelling of the parotids, erythematous candidiasis, petechia, gingivitis, xerostomia and angular cheilitis. Children with oral manifestations had almost normal counts of CD4+ T lymphocytes and high viral load.
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http://dx.doi.org/10.1590/s1517-74912002000100004DOI Listing
October 2002