Publications by authors named "Liliana Alzate-Gaviria"

14 Publications

  • Page 1 of 1

Decolorization of Textile Effluent by Trametes hirsuta Bm-2 and lac-T as Possible Main Laccase-Contributing Gene.

Curr Microbiol 2020 Dec 6;77(12):3953-3961. Epub 2020 Oct 6.

Department of Chemical and Biochemical Engineering, Tecnologico Nacional de Mexico/IT de Merida, Av. Tecnologico Km 4.5 S/N, 97118, Mérida, Yucatán, Mexico.

The decolorization of dye and textile effluent by Trametes hirsuta was studied in both induced and non-induced media. A removal of 70-100% of the color was achieved through adsorption and the action of laccases. Laccase activity was increased significantly with the addition of grapefruit peel (4000 U/mL) and effluent with grapefruit peel (16,000 U/mL) in comparison with the basal medium (50 U/mL). Analysis of the expression of laccase isoenzymes lac-B and lac-T revealed clear differences in the expression of these genes. The low levels of expression of lac-B in all media suggest a basal or constitutive gene expression, whereas lac-T was over-expressed in the media with effluent, and showed an up/down regulation depending on culture conditions and time. The results obtained suggest that the lac-T gene of T. hirsuta is involved in the decolorization of dyes.
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http://dx.doi.org/10.1007/s00284-020-02188-9DOI Listing
December 2020

Consolidated Bioprocess for Bioethanol Production from Raw Flour of Seeds Using the Native Strain of Bm-2.

Microorganisms 2019 Oct 23;7(11). Epub 2019 Oct 23.

Renewable Energy Department, Yucatan Center for Scientific Research, Merida 97302, Mexico.

Consolidated bioprocessing (CBP), which integrates biological pretreatment, enzyme production, saccharification, and fermentation, is a promising operational strategy for cost-effective ethanol production from biomass. In this study, the use of a native strain of (Bm-2) was evaluated for bioethanol production from in a CBP. The raw seed flour obtained from the ramon tree contained 61% of starch, indicating its potential as a raw material for bioethanol production. Quantitative assays revealed that the Bm-2 strain produced the amylase enzyme with activity of 193.85 U/mL. The Bm-2 strain showed high tolerance to ethanol stress and was capable of directly producing ethanol from raw flour at a concentration of 13 g/L, with a production yield of 123.4 mL/kg flour. This study demonstrates the potential of Bm-2 for starch-based ethanol production in a consolidated bioprocess to be implemented in the biofuel industry. The residual biomass after fermentation showed an average protein content of 22.5%, suggesting that it could also be considered as a valuable biorefinery co-product for animal feeding.
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http://dx.doi.org/10.3390/microorganisms7110483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920830PMC
October 2019

Influence of two polarization potentials on a bioanode microbial community isolated from a hypersaline coastal lagoon of the Yucatan peninsula, in México.

Sci Total Environ 2019 Sep 10;681:258-266. Epub 2019 May 10.

Renewable Energy, Yucatan Center for Scientific Research (CICY), Carretera Sierra Papacal-Chuburná Puerto, Km 5, Sierra Papacal, Mérida, Yucatán CP 97302, Mexico. Electronic address:

In recent years, halotolerant biofilms have become a subject of interest for its application in Bioelectrochemical systems for wastewater treatment. To determine if the polarization potential affects the microbial community of a halotolerant bioanode, four bioanodes were poised at potentials of +0.34 V/SHE and - 0.16 V/SHE and the 16S rRNA gene was analyzed through a MiSeq (Ilumina) system. Oceanospirillum, Halomonas and Marinobacterium were the most predominant genus; no previous studies have reported the presence of Oceanospirillum in anodic biofilms. The fitness with the dataset for +0.34 V/SHE with a modified Butler Volmer Monod model, gives a value of K was 0.0002 (2.64 A m and 38% coulombic efficiency), indicating the fastest electrochemical reaction. Whereas that -0.16 V/SHE case, the high value of K (12.2 with 1.82 A m and 10% coulombic efficiency) indicated that the electron transfer was far from being reversible (Nernstian).
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http://dx.doi.org/10.1016/j.scitotenv.2019.05.120DOI Listing
September 2019

Nanoferrosonication: A novel strategy for intensifying the methanogenic process in sewage sludge.

Bioresour Technol 2019 Mar 8;276:318-324. Epub 2019 Jan 8.

Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Campus de Ciencias Exactas e Ingenierías, Periférico Norte, Km. 33.5, Tablaje Catastral 13615, Col. Chuburná de Hidalgo Inn, C.P. 97203 Mérida, Yucatán, Mexico. Electronic address:

In this work, the effect of coupling ultrasonic pretreatment with dosing of zero-valent iron nanoparticles (nanoferrosonication, "NFS") to improve the anaerobic digestion of sewage sludge was studied. Biochemical methane potential tests were conducted at 15,000 and 25,000 kJ/kg and their combinations with 2 and 7 mgFe/g. The biogas yield increased from 106 (control) to 143 (25,000 kJ/kg) and 308 mL/g with NFS (7 mgFe/g + 15,000 kJ/kg). The methane content increased from 55.6 to 66%, and the maximum VS removal was 11.5% at 7 mgFe/g + 15,000 kJ/kg. The results demonstrated that NFS was effective in intensifying the process.
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http://dx.doi.org/10.1016/j.biortech.2019.01.021DOI Listing
March 2019

Corn industrial wastewater (nejayote): a promising substrate in Mexico for methane production in a coupled system (APCR-UASB).

Environ Sci Pollut Res Int 2018 Jan 23;25(1):712-722. Epub 2017 Oct 23.

Renewable Energy Unit, Yucatan Center for Scientific Research (CICY), Street 43 N.130 Col. Chuburná de Hidalgo, 97205, Merida, Yucatan, Mexico.

In Mexico, the corn tortilla is a food of great economic importance. Corn tortilla production generates about 1500-2000 m of wastewater per 600 tons of processed corn. Although this wastewater (also known as nejayote) has a high organic matter content, few studies in Mexico have analyzed its treatment. This study presents fresh data on the potential methane production capacity of nejayote in a two-phase anaerobic digestion system using an Anaerobic-Packed Column Reactor (APCR) to optimize the acidogenic phase and an up-flow anaerobic sludge blanket (UASB) reactor to enhance the methanogenic process. Results indicate that day 8 was ideal to couple the APCR to the UASB reactor. This allowed for a 19-day treatment that yielded 96% COD removal and generated a biogas containing 84% methane. The methane yield was 282 L kg of COD. Thus, two-phase anaerobic digestion is an efficient process to treat nejayote; furthermore, this study demonstrated the possibility of using an industrial application by coupling the APCR to the UASB reactor system, in order to assess its feasibility for biomethane generation as a sustainable bioenergy source.
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http://dx.doi.org/10.1007/s11356-017-0479-zDOI Listing
January 2018

Pretreatment of vinasse from the sugar refinery industry under non-sterile conditions by in a fluidized bed bioreactor and its effect when coupled to an UASB reactor.

J Biol Eng 2017 23;11. Epub 2017 Jan 23.

Yucatan center for scientific research (CICY), Renewable Energy Unit and Biotechnology Unit. Street 43 N.130 Col. Chuburná de Hidalgo 97200, Merida, Yucatan Mexico.

Background: During hydrous ethanol production from the sugar refinery industry in Mexico, vinasse is generated. Phenolic compounds and melanoidins contribute to its color and make degradation of the vinasse a difficult task. Although anaerobic digestion (AD) is feasible for vinasse treatment, the presence of recalcitrant compounds can be toxic or inhibitory for anaerobic microorganism. Therefore, this study presents new data on the coupled of the FBR (Fluidized Bed Bioreactor) to the UASB (Upflow Anaerobic Sludge Blanket) reactor under non-sterile conditions by . Nevertheless, for an industrial application, it is necessary to evaluate the performance in this kind of proposal system.

Results: Therefore, this study used a FBR for the removal of phenolic compounds (67%) and COD (38%) at non-sterile conditions. Continuous operation of the FBR was successfully for 26 days according to the literature. When the FBR was coupled to the UASB reactor, we obtained a better quality of effluent, furthermore methane content and yield were 74% and 0.18 m CH/ kg COD respectively.

Conclusions: This study demonstrated the possibility of using for an industrial application the coupled of the FBR to the UASB reactor under non-sterile conditions. Continuous operation of the FBR was carried out successfully for 26 days, which is the highest value found in the literature.
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http://dx.doi.org/10.1186/s13036-016-0042-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5259878PMC
January 2017

Removal of organic matter and electricity generation of sediments from Progreso, Yucatan, Mexico, in a sediment microbial fuel cell.

Environ Sci Pollut Res Int 2017 Feb 7;24(6):5868-5876. Epub 2017 Jan 7.

Unidad de Energía Renovable, Centro de Investigación Científica de Yucatán, Carretera Sierra Papacal-Chuburná Puerto Km 5, 97302, Sierra Papacal, Yucatán, Mexico.

Sediment microbial fuel cells (SMFCs) are devices that generate electrical energy through sediments rich in organic matter (OM). The present study assessed the potential of sediments collected at two sites in Yucatan, Mexico, (the swamp of Progreso port and Yucalpetén dock) to be used in these electrochemical devices. Sediments were collected during the rainy and winter seasons and were monitored in the SMFC for 120 days through electrochemical and physicochemical characterization. OM removal in the SMFC ranged from 8.1-18.01%, generating a maximum current density of 232.46 mA/cm and power density of 95.85 mW/cm. SUVA analysis indicated that with a young soil, the ratio E4/E6 presented evidence directly related to the degradation of aromatic and aliphatic compound formation, implying humification and, therefore, sediment enrichment.
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http://dx.doi.org/10.1007/s11356-016-8286-5DOI Listing
February 2017

A fast linear predictive adaptive model of packed bed coupled with UASB reactor treating onion waste to produce biofuel.

Microb Cell Fact 2016 Oct 3;15(1):167. Epub 2016 Oct 3.

Renewable Energy Unit, Yucatan Center for Scientific Research (CICY), Calle 40 No. 130, Colonia Chuburná de Hidalgo, 97200, Mérida, Yucatán, Mexico.

Background: Agro-industrial wastes are an energy source for different industries. However, its application has not reached small industries. Previous and current research activities performed on the acidogenic phase of two-phase anaerobic digestion processes deal particularly with process optimization of the acid-phase reactors operating with a wide variety of substrates, both soluble and complex in nature. Mathematical models for anaerobic digestion have been developed to understand and improve the efficient operation of the process. At present, lineal models with the advantages of requiring less data, predicting future behavior and updating when a new set of data becomes available have been developed. The aim of this research was to contribute to the reduction of organic solid waste, generate biogas and develop a simple but accurate mathematical model to predict the behavior of the UASB reactor.

Results: The system was maintained separate for 14 days during which hydrolytic and acetogenic bacteria broke down onion waste, produced and accumulated volatile fatty acids. On this day, two reactors were coupled and the system continued for 16 days more. The biogas and methane yields and volatile solid reduction were 0.6 ± 0.05 m (kg VS), 0.43 ± 0.06 m (kg VS) and 83.5 ± 9.8 %, respectively. The model application showed a good prediction of all process parameters defined; maximum error between experimental and predicted value was 1.84 % for alkalinity profile.

Conclusions: A linear predictive adaptive model for anaerobic digestion of onion waste in a two-stage process was determined under batch-fed condition. Organic load rate (OLR) was maintained constant for the entire operation, modifying effluent hydrolysis reactor feed to UASB reactor. This condition avoids intoxication of UASB reactor and also limits external buffer addition.
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http://dx.doi.org/10.1186/s12934-016-0563-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048648PMC
October 2016

Laccase Gene Expression and Vinasse Biodegradation by Trametes hirsuta Strain Bm-2.

Molecules 2015 Aug 19;20(8):15147-57. Epub 2015 Aug 19.

Departamento de Ingeniería Química y Bioquímica, Instituto Tecnológico de Mérida, Av. Tecnológico Km 5, Mérida 97118, Yucatán, Mexico.

Vinasse is the dark-colored wastewater that is generated by bioethanol distilleries from feedstock molasses. The vinasse that is generated from molasses contains high amounts of pollutants, including phenolic compounds and melanoindin. The goal of this work was to study the expression of laccase genes in the Trametes hirsuta strain Bm-2, isolated in Yucatan, Mexico, in the presence of phenolic compounds, as well as its effectiveness in removing colorants from vinasse. In the presence of all phenolic compounds tested (guaiacol, ferulic acid, and vanillic acid), increased levels of laccase-encoding mRNA were observed. Transcript levels in the presence of guaiacol were 40 times higher than those in the control. The lcc1 and lcc2 genes of T. hirsuta were differentially expressed; guaiacol and vanillin induced the expression of both genes, whereas ferulic acid only induced the expression of lcc2. The discoloration of vinasse was concomitant with the increase in laccase activity. The highest value of enzyme activity (2543.7 U/mL) was obtained in 10% (v/v) vinasse, which corresponded to a 69.2% increase in discoloration. This study demonstrates the potential of the Bm-2 strain of T. hirsuta for the biodegradation of vinasse.
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http://dx.doi.org/10.3390/molecules200815147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332155PMC
August 2015

Pluggable microbial fuel cell stacks for septic wastewater treatment and electricity production.

Bioresour Technol 2015 Mar 10;180:258-63. Epub 2015 Jan 10.

Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, Boulder, CO, USA. Electronic address:

Septic tanks and other decentralized wastewater treatment systems play an important role in protecting public health and water resource for remote or developing communities. Current septic systems do not have energy production capability, yet such feature can be very valuable for areas lack access to electricity. Here we present an easy-to-operate microbial fuel cell (MFC) stack that consists a common base and multiple pluggable units, which can be connected in either series or parallel for electricity generation during waste treatment in septic tanks. Lab studies showed such easy configuration obtained a power density of 142±6.71mWm(-2) when 3 units are connected in parallel, and preliminary calculation indicates that a system that costs approximately US $25 can power a 6-watt LED light for 4h per day with great improvement potential. Detailed electrochemical characterizations provide insights on system internal loss and technology advancement needed.
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http://dx.doi.org/10.1016/j.biortech.2014.12.100DOI Listing
March 2015

Characterization of anode and anolyte community growth and the impact of impedance in a microbial fuel cell.

BMC Biotechnol 2014 Dec 9;14:102. Epub 2014 Dec 9.

Renewable Energy Unit, Centro de Investigación Científica de Yucatán A.C (CICY), Calle 43 No. 130 Col. Chuburná de Hidalgo, C.P. 97200, Mérida, Yucatán, México.

Background: A laboratory-scale two-chamber microbial fuel cell employing an aerated cathode with no catalyst was inoculated with mixed inoculum and acetate as the carbon source. Electrochemical impedance spectroscopy (EIS) was used to study the behavior of the MFC during initial biofilm (week 1) and maximum power density (week 20). EIS were performed on the anode chamber, biofilm (without anolyte) and anolyte (without biofilm). Nyquist plots of the EIS data were fitted with two equivalent electrical circuits to estimate the contributions of intrinsic resistances to the overall internal MFC impedance at weeks 1 and 20, respectively.

Results: The results showed that the system tended to increase power density from 15 ± 3 (week 1) to 100 ± 15 mW/m(2) (week 20) and current density 211 ± 7 (week 1) to 347 ± 29 mA/m(2) (week 20). The Samples were identified by pyrosequencing of the 16S rRNA gene and showed that initial inoculum (week 1) was constituted by Proteobacteria (40%), Bacteroidetes (22%) and Firmicutes (18%). At week 20, Proteobacterial species were predominant (60%) for electricity generation in the anode biofilm, being 51% Rhodopseudomonas palustris. Meanwhile on anolyte, Firmicutes phylum was predominant with Bacillus sp. This study proved that under the experimental conditions used there is an important contribution from the interaction of the biofilm and the anolyte on cell performance. Table 1 presents a summary of the specific influence of each element of the system under study.

Conclusions: The results showed certain members of the bacterial electrode community increased in relative abundance from the initial inoculum. For example, Proteobacterial species are important for electricity generation in the anode biofilms and Firmicutes phylum was predominant on anolyte to transfer electron. R1 is the same in the three systems and no variation is observed over time. The biofilm makes a significant contribution to the charge transfer processes at the electrode (R2 and Cdl) and, consequently, on the performance of the anode chamber. The biofilm can act as a barrier which reduces diffusion of the anolyte towards the electrode, all the while behaving like a porous material. The anolyte and its interaction with the biofilm exert a considerable influence on diffusion processes, given that it presents the highest values for Rd which increased at week 20.
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http://dx.doi.org/10.1186/s12896-014-0102-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4299683PMC
December 2014

Methane production by treating vinasses from hydrous ethanol using a modified UASB reactor.

Biotechnol Biofuels 2012 Nov 21;5(1):82. Epub 2012 Nov 21.

Unidad de Energía Renovable, Centro de Investigación Científica de Yucatán A,C (CICY), Calle 43 No, 130 Col, Chuburná de Hidalgo, C,P, 97200, Mérida, Yucatán, Mexico.

Unlabelled:

Background: A modified laboratory-scale upflow anaerobic sludge blanket (UASB) reactor was used to obtain methane by treating hydrous ethanol vinasse. Vinasses or stillage are waste materials with high organic loads, and a complex composition resulting from the process of alcohol distillation. They must initially be treated with anaerobic processes due to their high organic loads. Vinasses can be considered multipurpose waste for energy recovery and once treated they can be used in agriculture without the risk of polluting soil, underground water or crops. In this sense, treatment of vinasse combines the elimination of organic waste with the formation of methane. Biogas is considered as a promising renewable energy source. The aim of this study was to determine the optimum organic loading rate for operating a modified UASB reactor to treat vinasse generated in the production of hydrous ethanol from sugar cane molasses.

Results: The study showed that chemical oxygen demand (COD) removal efficiency was 69% at an optimum organic loading rate (OLR) of 17.05 kg COD/m3-day, achieving a methane yield of 0.263 m3/kg CODadded and a biogas methane content of 84%. During this stage, effluent characterization presented lower values than the vinasse, except for potassium, sulfide and ammonia nitrogen. On the other hand, primers used to amplify the 16S-rDNA genes for the domains Archaea and Bacteria showed the presence of microorganisms which favor methane production at the optimum organic loading rate.

Conclusions: The modified UASB reactor proposed in this study provided a successful treatment of the vinasse obtained from hydrous ethanol production.Methanogen groups (Methanobacteriales and Methanosarcinales) detected by PCR during operational optimum OLR of the modified UASB reactor, favored methane production.
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http://dx.doi.org/10.1186/1754-6834-5-82DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538563PMC
November 2012

Vinasses: characterization and treatments.

Waste Manag Res 2011 Dec 17;29(12):1235-50. Epub 2011 Jan 17.

Unidad de Energía Renovable, Centro de Investigación Científica de Yucatán A. C. (CICY), Mérida, Yucatán, México.

The final products of the ethanol industry are alcoholic beverages, industrial ethanol and biofuels. They are produced by the same production process, which includes fermentation and distillation of raw materials which come from plant biomass. At the end of the distillation process a waste effluent is obtained called vinasse or stillage. The direct disposal of stillages on land or in groundwater (rivers, streams or lakes), or even for the direct irrigation of crops, pollutes the environment due to their high organic contents, dissolved solids and many other compounds which are toxic or could be contaminants under certain environmental conditions. This work reviews the characterization of vinasses from different feedstock sources and the main treatments for conditioning the soluble solids of vinasses before their disposal.
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http://dx.doi.org/10.1177/0734242X10387313DOI Listing
December 2011

A side-by-side comparison of two systems of sequencing coupled reactors for anaerobic digestion of the organic fraction of municipal solid waste.

Waste Manag Res 2005 Jun;23(3):270-80

CINVESTAV-IPN., Department of Biotechnology and Bioengineering, Environmental Biotechnology R&D Group, P.O. Box 14-740, México DF, 07000, México.

The objective of this work was to compare the performance of two laboratory-scale, mesophilic systems aiming at the anaerobic digestion of the organic fraction of municipal solid wastes (OFMSW). The first system consisted of two coupled reactors packed with OFMSW (PBR1.1-PBR1.2) and the second system consisted of an upflow anaerobic sludge bed reactor (UASB) coupled to a packed reactor (UASB2.1-PBR2.2). For the start-up phase, both reactors PBR 1.1 and the UASB 2.1 (also called leading reactors) were inoculated with a mixture of non-anaerobic inocula and worked with leachate and effluent full recirculation, respectively. Once a full methanogenic regime was achieved in the leading reactors, their effluents were fed to the fresh-packed reactors PBR1.2 and PBR2.2, respectively. The leading PBR 1.1 reached its full methanogenic regime after 118 days (Tm, time to achieve methanogenesis) whereas the other leading UASB 2.1 reactor reached its full methanogenesis regime after only 34 days. After coupling the leading reactors to the corresponding packed reactors, it was found that both coupled anaerobic systems showed similar performances regarding the degradation of the OFMSW. Removal efficiencies of volatile solids and cellulose and the methane pseudo-yield were 85.95%, 80.88% and 0.109 NL CH4 g(-1) VS(fed) in the PBR-PBR system; and 88.75%, 82.61% and 0.115 NL CH4 g(-1) VS(fed0 in the UASB-PBR system [NL, normalized litre (273 degrees K, 1 ata basis)]. Yet, the second system UASB-PBR system showed a faster overall start-up.
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http://dx.doi.org/10.1177/0734242X05054166DOI Listing
June 2005