Publications by authors named "Lili Yang"

566 Publications

CBD Alleviates Liver Injuries in Alcoholics With High-Fat High-Cholesterol Diet Through Regulating NLRP3 Inflammasome-Pyroptosis Pathway.

Front Pharmacol 2021 22;12:724747. Epub 2021 Sep 22.

Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou, China.

Alcohol abuse and high-fat diet-induced liver diseases have been the most prevalent chronic liver diseases and the leading reasons for liver transplantation around the world. Cannabidiol (CBD) is a botanical component extracted from marijuana plants without psychoactive impact. In our previous reports, we found that CBD can prevent fatty liver induced by Lieber-DeCarli ethanol diet or non-alcoholic fatty liver disease (NAFLD) induced by high-fat high-cholesterol diet. The current work is a further study on whether CBD can alleviate liver injuries induced by ethanol plus high-fat high-cholesterol diet (EHFD), which is a model simulating heavy alcohol drinkers in a Western diet. A mice liver injury model induced by EHFD for 8 weeks was applied to explore the protective properties of CBD and the underlying mechanisms. We found that CBD prevented liver steatosis and oxidative stress induced by EHFD. CBD treatment inhibited macrophage recruitment and suppressed activation of NFκB-NLRP3-pyroptosis pathway in mice livers. The hepatoprotective property of CBD in the current model might be a result of inhibition of inflammation via alleviating activation of the hepatic NFκB-NLRP3 inflammasome-pyroptosis pathway by CBD.
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http://dx.doi.org/10.3389/fphar.2021.724747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8493333PMC
September 2021

Indoor Exposure to Products of Incomplete Combustion of Household Fuels in Rural Tibetan Plateau.

Environ Sci Technol 2021 Sep 30. Epub 2021 Sep 30.

School of the Environment, Hangzhou Institute for Advanced Study, University of the Chinese Academy of Sciences, Hangzhou 310024, China.

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http://dx.doi.org/10.1021/acs.est.1c05298DOI Listing
September 2021

Structural and emulsion stabilization comparison of four gelatins from two freshwater and two marine fish skins.

Food Chem 2021 Sep 14;371:131129. Epub 2021 Sep 14.

National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Integrated Scientific Research Base on Comprehensive Utilization Technology for By-Products of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, Liaoning Province, China. Electronic address:

This study analyzed the structural and emulsion stabilization properties of two freshwater and two marine fish skin gelatins: Chinese longsnout catfish skin gelatin (CLCSG), silver carp skin gelatin (SCSG), salmon skin gelatin (SSG), and Alaska pollack skin gelatin (APSG). Their gel strengths (Bloom values) were: 361 ± 1 (SCSG) > 253 ± 4 (CLCSG) > 69 ± 1 (SSG) > 36 ± 2 (APSG). Higher molecular weights and α/β subunit contents of gelatins might induce higher gel strengths. Both creaming and droplet stability were completely the same to the contents of imino acids, β-sheet percentages, and β-turn percentages, whereas they were completely the opposite to random coil percentages. The emulsion stabilization mechanisms involved an "fish skin source - protein chemical composition - protein secondary structure - protein functional properties - emulsion stability" route. This study provided useful knowledges for gelatin science and for the comprehensive utilization of aquatic by-products in gelatin industry.
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http://dx.doi.org/10.1016/j.foodchem.2021.131129DOI Listing
September 2021

Bridging the Energy Benefit and POPs Emission Risk from Waste Incineration.

Innovation (N Y) 2021 Feb 30;2(1):100075. Epub 2020 Dec 30.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.

Incineration has been the globally controversial and concerned method of solid waste disposal. Energy recovery and volume reduction are the benefits from waste incineration, but risk due to release of persistent organic pollutants is the major public concern in the world. In this study, the emission of organic pollutants including dioxins and polychlorinated naphthalenes from solid waste incineration in China was comprehensively evaluated, and a relationship between energy benefit and pollutant emission was firstly established. The results show that production of medical and industrial waste was smaller than that of municipal waste but yielded comparable or even higher emission of dioxins. The energy benefit-to-emission index for organic pollutants (EBEI) for evaluation of solid waste management on a local or regional scale was proposed. Significant correlations between net energy benefit and pollutant emission for provinces with higher EBEI values were found. Furthermore, higher EBEI values were associated with economic factors while lower values were influenced by emission from incineration of medical and industrial waste. We suggest that an EBEI value of ≥60 can serve as a reference for "profitable" solid waste management, assisting decision making during energy benefit and environmental risk assessment.
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http://dx.doi.org/10.1016/j.xinn.2020.100075DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8454607PMC
February 2021

Signatures of Multi-Omics Reveal Distinct Tumor Immune Microenvironment Contributing to Immunotherapy in Lung Adenocarcinoma.

Front Immunol 2021 3;12:723172. Epub 2021 Sep 3.

Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.

Background: Lung adenocarcinoma (LUAD) contains a variety of genomic and epigenomic abnormalities; the effective tumor markers related to these abnormalities need to be further explored.

Methods: Clustering analysis was performed based on DNA methylation (MET), DNA copy number variation (CNV), and mRNA expression data, and the differences in survival and tumor immune microenvironment (TIME) between subtypes were compared. Further, we evaluated the signatures in terms of both prognostic value and immunological characteristics.

Results: There was a positive correlation between MET and CNV in LUAD. Integrative analysis of multi-omics data from 443 samples determined molecular subtypes, iC1 and iC2. The fractions of CD8+ T cells and activated CD4+ T cells were higher, the fraction of Tregs was lower, and the expression level of programmed death-ligand 1 (PD-L1) was higher in iC2 with a poor prognosis showing a higher TIDE score. We selected , , and as signatures of molecular subtypes to build a prognostic risk model and divided patients into high-risk group and low-risk group representing poor prognosis and good prognosis, respectively, which were validated in 180 patients with LUAD. Further, the low-risk group with lower TIDE score had more infiltrating immune cells. In 100 patients with LUAD, the high-risk group with an immunosuppressive state had a higher expression of PD-L1 and lower counts of CD8+ T cells and dendritic cells.

Conclusions: These findings demonstrated that combined multi-omics data could determine molecular subtypes with significant differences of prognosis and TIME in LUAD and suggested potent utility of the signatures to guide immunotherapy.
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http://dx.doi.org/10.3389/fimmu.2021.723172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446514PMC
September 2021

Congener profiles and process distributions of polychlorinated biphenyls, polychlorinated naphthalenes and chlorinated polycyclic aromatic hydrocarbons from secondary copper smelting.

J Hazard Mater 2021 Sep 9;423(Pt B):127125. Epub 2021 Sep 9.

School of Environment, Hangzhou Institute for Advanced Study, UCAS, Hangzhou 310024, China; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address:

Secondary copper smelting is an important industrial source of unintentionally produced persistent organic pollutants (UPOPs) emissions. Herein, field study on industrial-scale plants was conducted to clarify the levels and profiles of polychlorinated biphenyls (PCBs), polychlorinated naphthalenes (PCNs) and chlorinated polycyclic aromatic hydrocarbons (> 3 rings, Cl-PAHs) from secondary copper smelting plants. The three UPOPs emission levels from the oxygen-enriched smelting furnace were higher than that from the anode furnace, which was attributed to the low-grade raw materials used. The toxic equivalent quantity concentrations of Cl-PAHs were 1.3-4.4 and 4.6-18.9 times higher than that of PCBs and PCNs, respectively. Thus, the emission control of Cl-PAHs in the secondary copper industry should be of concern. The chlorination degree of PCBs and PCNs was ~4 after the gas-cooling stage but was reduced to 1-2 in the stack outlet. This result indicated that the PCBs and PCNs congeners that were generated during the cooling stage were mainly higher-chlorinated. After purification by air pollution control devices (APCDs), the high-chlorinated congeners were removed simultaneously with the fly ash, whereas the low-chlorinated congeners may be regenerated and transferred into the stack gas due to possible memory effect within the APCDs.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127125DOI Listing
September 2021

Methods for Studying Mouse and Human Invariant Natural Killer T Cells.

Methods Mol Biol 2021 ;2388:35-57

Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA.

Invariant natural killer T (iNKT) cells are a unique subset of T lymphocytes that recognize lipid antigens presented by nonpolymorphic major histocompatibility complex (MHC) I-like molecule CD1d. iNKT cells play essential roles in regulating immune responses against cancer, viral infection, autoimmune disease, and allergy. However, the study and application of iNKT cells have been hampered by their very small numbers (0.01-1% in mouse and human blood). Here, we describe protocols to (1) generate mouse iNKT cells from mouse mononuclear cells or from mouse hematopoietic stem cells engineered with iNKT T cell receptor (TCR) gene (denoted as mMNC-iNKT cells or mHSC-iNKT cells, respectively), (2) generate human iNKT cells from human peripheral blood mononuclear cells or from human HSC cells engineered with iNKT TCR gene (denoted as hPBMC-iNKT cells or hHSC-iNKT cells, respectively), and (3) characterize mouse and human iNKT cells in vitro and in vivo.
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http://dx.doi.org/10.1007/978-1-0716-1775-5_4DOI Listing
January 2021

A novel delivery nanobiotechnology: engineered miR-181b exosomes improved osteointegration by regulating macrophage polarization.

J Nanobiotechnology 2021 Sep 7;19(1):269. Epub 2021 Sep 7.

Spine Center, Department of Orthopaedics, Shanghai Changzheng Hospital, Second Affiliated Hospital of Naval Medical University, 200003, Shanghai, China.

Background: Many patients suffer from implant loosening after the implantation of titanium alloy caused by immune response to the foreign bodies and this could inhibit the following osteogenesis, which could possibly give rise to aseptic loosening and poor osteointegration while there is currently no appropriate solution in clinical practice. Exosome (Exo) carrying miRNA has been proven to be a suitable nanocarrier for solving this problem. In this study, we explored whether exosomes overexpressing miR-181b (Exo-181b) could exert beneficial effect on promoting M2 macrophage polarization, thus inhibiting inflammation as well as promoting osteogenesis and elaborated the underlying mechanism in vitro. Furthermore, we aimed to find whether Exo-181b could enhance osteointegration.

Results: In vitro, we firstly verified that Exo-181b significantly enhanced M2 polarization and inhibited inflammation by suppressing PRKCD and activating p-AKT. Then, in vivo, we verified that Exo-181b enhanced M2 polarization, reduced the inflammatory response and enhanced osteointegration. Also, we verified that the enhanced M2 polarization could indirectly promote the migration and osteogenic differentiation by secreting VEGF and BMP-2 in vitro.

Conclusions: Exo-181b could suppress inflammatory response by promoting M2 polarization via activating PRKCD/AKT signaling pathway, which further promoting osteogenesis in vitro and promote osteointegration in vivo.
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http://dx.doi.org/10.1186/s12951-021-01015-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8424816PMC
September 2021

Engineering stem cells for cancer immunotherapy.

Trends Cancer 2021 Aug 31. Epub 2021 Aug 31.

Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA; Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA. Electronic address:

Engineering stem cells presents an attractive paradigm for cancer immunotherapy. Stem cells engineered to stably express various chimeric antigen receptors (CARs) or T-cell receptors (TCRs) against tumor-associated antigens are showing increasing promise in the treatment of solid tumors and hematologic malignancies. Stem cells engraft for long-term immune cell generation and serve as a sustained source of tumor-specific effector cells to maintain remissions. Furthermore, engineering stem cells provides 'off-the-shelf' cellular products, obviating the need for a personalized and patient-specific product that plagues current autologous cell therapies. Herein, we summarize recent progress of stem cell-engineered cancer therapies, and discuss the utility, impact, opportunities, and challenges of cellular engineering that may facilitate the translational and clinical research.
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http://dx.doi.org/10.1016/j.trecan.2021.08.004DOI Listing
August 2021

The prognostic landscape of genes and infiltrating immune cells in cytokine induced killer cell treated-lung squamous cell carcinoma and adenocarcinoma.

Cancer Biol Med 2021 Aug 30. Epub 2021 Aug 30.

Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Immunology and Biotherapy, Tianjin 300060, China.

Objective: Patients with non-small cell lung cancer (NSCLC) respond differently to cytokine-induced killer cell (CIK) treatment. Therefore, potential prognostic markers to identify patients who would benefit from CIK treatment must be elucidated. The current research aimed at identifying predictive prognostic markers for efficient CIK treatment of patients with NSCLC.

Methods: Patients histologically diagnosed with NSCLC were enrolled from the Tianjin Medical University Cancer Institute and Hospital. We performed whole-exome sequencing (WES) on the tumor tissues and paired adjacent benign tissues collected from 50 patients with NSCLC, and RNA-seq on tumor tissues of 17 patients with NSCLC before CIK immunotherapy treatment. Multivariate Cox proportional hazard regression analysis was used to analyze the association between clinical parameters and prognostic relevance. WES and RNA-seq data between lung squamous cell carcinoma (SCC) and adenocarcinoma (Aden) were analyzed and compared.

Results: The pathology subtype of lung cancer was the most significantly relevant clinical parameter associated with DFS, as analyzed by multivariate Cox proportional hazard regression ( = 0.031). The patients with lung SCC showed better CIK treatment efficacy and extended DFS after CIK treatment. Relatively low expression of HLA class II genes and checkpoint molecules, and less immunosuppressive immune cell infiltration were identified in the patients with lung SCC.

Conclusions: Coordinated suppression of the expression of HLA class II genes and checkpoint molecules, as well as less immune suppressive cell infiltration together contributed to the better CIK treatment efficacy in lung SCC than lung Aden.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2021.0023DOI Listing
August 2021

Somatic copy number alterations are predictive of progression-free survival in patients with lung adenocarcinoma undergoing radiotherapy.

Cancer Biol Med 2021 Aug 27. Epub 2021 Aug 27.

Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Key Laboratory of Cancer Immunology and Biotherapy, Tianjin 300060, China.

Objective: Lung cancer is the most common cause of cancer-related deaths worldwide. Somatic copy number alterations (SCNAs) have been used to predict responses to therapies in many cancers, including lung cancer. However, little is known about whether they are predictive of radiotherapy outcomes. We aimed to understand the prognostic value and biological functions of SCNAs.

Methods: We analyzed the correlation between SCNAs and clinical outcomes in The Cancer Genome Atlas data for 486 patients with non-small cell lung cancer who received radiotherapy. Gene set enrichment analyses were performed to investigate the potential mechanisms underlying the roles of SCNAs in the radiotherapy response. Our results were validated in 20 patients with lung adenocarcinoma (LUAD) receiving radiotherapy.

Results: SCNAs were a better predictor of progression-free survival (PFS) in LUAD ( = 0.024) than in lung squamous carcinoma ( = 0.18) in patients treated with radiotherapy. Univariate and multivariate regression analyses revealed the superiority of SCNAs in predicting PFS in patients with LUAD. Patients with stage I cancer and low SCNA levels had longer PFS than those with high SCNA levels ( = 0.022). Our prognostic nomogram also showed that combining SCNAs and tumor/node/metastasis provided a better model for predicting long-term PFS. Additionally, high SCNA may activate the cell cycle pathway and induce tumorigenesis.

Conclusions: SCNAs may be used to predict PFS in patients with early-stage LUAD with radiotherapy, in combination with TNM, with the aim of predicting long-term PFS. Therefore, SCNAs are a novel predictive biomarker for radiotherapy in patients with LUAD.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0728DOI Listing
August 2021

Dicyanamide Anion-Based Ionic Liquid-Functionalized Graphene-Supported Pt Catalysts for Boosting Methanol Electrooxidation.

Inorg Chem 2021 Sep 26;60(17):13736-13747. Epub 2021 Aug 26.

State Key Laboratory of Chemistry and Utilization of Carbon-based Energy Resource, Key Laboratory of Energy Materials Chemistry, Ministry of Education, Key Laboratory of Advanced Functional Materials, Autonomous Region; Institute of Applied Chemistry, College of Chemistry, Xinjiang University, Urumqi 830046, Xinjiang, P. R. China.

As an environmentally friendly energy technology, direct methanol fuel cells (DMFCs) meet the needs of sustainable development. Herein, novel dicyanamide anion-based (N(CN)) ionic liquid (IL)-functionalized reduced graphene oxide (rGO)-supported Pt catalysts are synthesized via a facile one-pot room temperature reduction method, which show a boost in methanol oxidation performance compared with Pt/rGO. The mass activities of the as-prepared Pt/emimN(CN)/rGO (863.6 mA mg) and Pt/epyN(CN)/rGO (524.9 mA mg) are about five and three times higher than that of Pt/rGO (178.6 mA mg), and about six and four times higher than that of Pt/C (140.2 mA mg), respectively. The participation of ILs significantly improves the CO poisoning resistance, stability, and activity for methanol oxidation of catalysts. The relationship between the structures and conductivities of diverse ILs and the performance of Pt catalysts are studied systematically. These findings may offer a promising prospect of ILs in DMFCs.
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http://dx.doi.org/10.1021/acs.inorgchem.1c02111DOI Listing
September 2021

Efficacy and Mechanism of Electroacupuncture Treatment of Rabbits With Different Degrees of Knee Osteoarthritis: A Study Based on Synovial Innate Immune Response.

Front Physiol 2021 5;12:642178. Epub 2021 Aug 5.

Acupuncture and Moxibustion Department, The Third Hospital Affiliated to Beijing University of Chinese Medicine, Beijing, China.

Knee osteoarthritis (KOA) is a chronic degenerative bone and joint disease, which is often clinically manifested as pain, joint swelling, and deformity. Its pathological manifestations are mainly synovial inflammation and cartilage degeneration. This study aims to investigate the efficacy of electro-acupuncture (EA) on model rabbits with varying degrees of KOA and to study the mechanism of EA on KOA based on the innate immune response. Mild and moderate rabbit KOA models were established using a modified Hluth method, and EA was given to both the mild and moderate model groups. The Lequesne-MG index was used to evaluate the behavioral changes in the rabbits before and after EA treatment. Morphological changes in the synovial membrane and cartilage of each group were observed by H&E staining. The Mankin scoring standard and the Krenn scoring standard were used to score the pathology of the cartilage tissue and synovial tissue, respectively. The inflammatory factors and metalloproteinases were detected in the serum of each group by ELISA. The protein and messenger RNA (mRNA) expressions of important elements related to Toll-like receptors (TLRs)-mediated innate immune response in the synovial tissue were detected by Western blot and quantitative PCR (qPCR). The Lequesne-MG index score of the rabbits gradually increased with the modeling prolonged but decreased significantly after EA treatment, indicating that EA has a better effect on alleviating the pain and improving the dysfunction. The morphological analysis showed that the inflammation of and the damage to the synovial membrane and the cartilage tissue gradually deteriorated with the modeling prolonged. However, the synovial membrane inflammation was significantly relieved after EA treatment, and the cartilage injury showed signs of repair. The ELISA analysis showed that, with the modeling prolonged, the serum-related inflammatory factors and mechanism of metalloproteinases gradually increased but decreased after EA treatment. The tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and matrix metalloproteinase3 (MMP3) of EA1 group were significantly lower than those of EA2 group. Both Western blot and qPCR results showed that the protein and mRNA expressions of the elements related to the innate immune response in the synovial membrane increased gradually with the modeling prolonged, but decreased significantly after EA treatment. Additionally, the expression of some components in EA1 group was significantly lower than that in EA2 group. These results confirm that synovial inflammation gradually aggravated with time from the early to mid-stage of KOA. EA alleviated the inflammation and histological changes in KOA rabbits by inhibiting the TLRs-mediated innate synovial immune response. This suggests that using EA in the early stage of KOA may achieve a desirable efficacy.
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http://dx.doi.org/10.3389/fphys.2021.642178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375319PMC
August 2021

17β-Estradiol activates Cl channels via the estrogen receptor α pathway in human thyroid cells.

Channels (Austin) 2021 Dec;15(1):516-527

Department of Physiology, Medical College, Jinan University, Guangzhou, China.

Estradiol regulates thyroid function, and chloride channels are involved in the regulation of thyroid function. However, little is known about the role of chloride channels in the regulation of thyroid functions by estrogen. In this study, the effects of estrogen on chloride channel activities in human thyroid Nthy-ori3-1 cells were therefore investigated using the whole cell patch-clamp technique. The results showed that the extracellular application of 17β-estradiol (E2) activated Cl currents, which reversed at a potential close to Cl equilibrium potential and showed remarkable outward rectification and an anion permeability of I > Br > Cl > gluconate. The Cl currents were inhibited by the chloride channel blockers, NPPB and tamoxifen. Quantitative Real-time PCR results demonstrated that ClC-3 expression was highest in ClC family member in Nthy-ori3-1 cells. The down-regulation of ClC-3 expression by ClC-3 siRNA inhibited E2-induced Cl current. The Cl current was blocked by the estrogen receptor antagonist, ICI 182780 (fulvestrant). Estrogen receptor alpha (ERα) and not estrogen receptor beta was the protein expressed in Nthy-ori3-1 cells, and the knockdown of ERα expression with ERα siRNA abolished E2-induced Cl currents. Estradiol can promote the accumulation of ClC-3 in cell membrane. ERα and ClC-3 proteins were partially co-localized in the cell membrane of Nthy-ori3-1 cells after estrogen exposure. The results suggest that estrogen activates chloride channels via ERα in normal human thyroid cells, and ClC-3 proteins play a pivotal role in the activation of E2-induced Cl current.
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http://dx.doi.org/10.1080/19336950.2021.1957627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381838PMC
December 2021

Comparison of silver carp fin gelatins extracted by three types of methods: Molecular characteristics, structure, function, and pickering emulsion stabilization.

Food Chem 2022 Jan 11;368:130818. Epub 2021 Aug 11.

National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Integrated Scientific Research Base on Comprehensive Utilization Technology for By-Products of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, Liaoning Province, China. Electronic address:

High value-added utilization of different aquatic by-products is an increasingly urgent issue in aquatic science and industry. In this work, the effects of extraction methods on the molecular characteristics, structural properties, functional properties, and Pickering emulsion stabilization behaviors of silver carp fin gelatins were comprehensively studied. All the results showed molecular characteristics of silver carp fin gelatin was the key parameter to determine their functional properties such as wide gel strength range, excellent water-holding capacity, and excellent Pickering emulsion stabilization ability. The Pickering emulsion stabilization mechanisms of fin gelatins involved an "extraction method - protein molecular characteristics - fat-binding capacity - droplet structure - water phase properties - Pickering emulsion stability" route. This work could be helpful to understand the basic information on how the molecular characteristics determine the functions of gelatins. It would be also useful for the high value-added utilization of aquatic by-products and gelatins.
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http://dx.doi.org/10.1016/j.foodchem.2021.130818DOI Listing
January 2022

Safety and efficacy of anti-tau monoclonal antibody gosuranemab in progressive supranuclear palsy: a phase 2, randomized, placebo-controlled trial.

Nat Med 2021 08 12;27(8):1451-1457. Epub 2021 Aug 12.

Biogen, Cambridge, MA, USA.

A randomized, double-blind, placebo-controlled, 52-week study (no. NCT03068468) evaluated gosuranemab, an anti-tau monoclonal antibody, in the treatment of progressive supranuclear palsy (PSP). In total, 486 participants dosed were assigned to either gosuranemab (n = 321) or placebo (n = 165). Efficacy was not demonstrated on adjusted mean change of PSP Rating Scale score at week 52 between gosuranemab and placebo (10.4 versus 10.6, P = 0.85, primary endpoint), or at secondary endpoints, resulting in discontinuation of the open-label, long-term extension. Unbound N-terminal tau in cerebrospinal fluid decreased by 98% with gosuranemab and increased by 11% with placebo (P < 0.0001). Incidences of adverse events and deaths were similar between groups. This well-powered study suggests that N-terminal tau neutralization does not translate into clinical efficacy.
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http://dx.doi.org/10.1038/s41591-021-01455-xDOI Listing
August 2021

Prolonged Maternal Separation Induces the Depression-Like Behavior Susceptibility to Chronic Unpredictable Mild Stress Exposure in Mice.

Biomed Res Int 2021 29;2021:6681397. Epub 2021 Jul 29.

Department of Nephrology, Yangzhou Hospital Affiliated to Nanjing University of Chinese Medicine, Yangzhou 225002, China.

Early life stress is an important determinant for developing depression later in life. It is reported that maternal separation (MS) could trigger stress sensitivity in adulthood when exposed to stress again. However, it could also result in resilience to stress-induced depression. The conclusions are contradictory. To address this issue, C57BL/6N newborn pups were exposed to either daily short MS (MS for 15 min per day; MS15) or prolonged MS (MS for 180 min per day; MS180) from the first day postpartum (PD1) to PD21. Adult mice were then subjected to chronic unpredictable mild stress (CUMS) exposure from PD64 to PD105. The behavior tests such as the forced swimming test (FST), tail suspension test (TST), and open-field test were performed once a week during this time. Besides, the hippocampal neurosteroids, serum stress hormones, and hippocampal monoamine neurotransmitters were measured at PD106. We found that mice in the MS180 group displayed the reduced struggling time and the increased latency to immobility in both FST and TST. However, there was no significant difference in the MS15 group. The levels of hippocampal neurosteroids (progesterone and allopregnanolone) were decreased, and the serum levels of corticosterone, corticotropin-releasing hormone, and adrenocorticotropic hormone were overexpressed in the MS180 group. Besides, the expressions of monoamine neurotransmitters such as 5-hydroxytryptamine and 5-hydroxy indole acetic acid significantly decreased in the MS180 group, but not in the MS15 group. All findings revealed that prolonged MS, rather than short MS, could increase the susceptibility to depression-like behavior when reexposed to stress in adulthood. However, future studies are warranted to identify the underlying neuromolecular mechanism of the MS experience on the susceptibility to adult stress reexposure.
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http://dx.doi.org/10.1155/2021/6681397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342142PMC
September 2021

Interior/Interface Modification of Textured Perovskite for Enhanced Photovoltaic Outputs of Planar Solar Cells by an In Situ Growth Passivation Technology.

ACS Appl Mater Interfaces 2021 Aug 6;13(33):39689-39700. Epub 2021 Aug 6.

Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Changchun 130103, China.

To compensate for the photoelectric losses of planar heterojunction perovskite solar cells (PSCs), the development of high-quality textured absorbers with excellent light-harvesting ability and carrier extraction/transfer efficiency is of great significance to achieve a high-efficiency stable photovoltaic output. In this paper, we propose an in situ growth passivation technique to construct high-performance textured absorbers by adding a 2-amino-4-chlorophenol (AC) modifier consisting of multiple groups during the growth of textured perovskite. Initially, according to the Ostwald ripening mechanism, the strongly polar dimethylformamide (DMF) was used as the etchant to systematically study its synergistic effect on the morphology evolution, crystallization kinetics, light-trapping capability, and photovoltaic loss of textured absorbers. An appropriate amount of DMF induces formamidinium cations (FA) to replace methylammonium cations (MA) in the perovskite lattice while etching the absorber to form a texture configuration, which effectively broadens the spectral absorption range, thus greatly improving the light-trapping capacity and short-circuit current density of planar PSCs. In contrast, excess DMF deteriorates the device performance due to the excessive corrosion of the perovskite. Moreover, the introduction of the AC modifier is of great significance for passivating deep-level defects and accelerating the charge extraction/transfer. Owing to the electron-donating nature of the Lewis base, the hydroxyl groups with a higher electron density in AC molecules can better coordinate with Pb ion defects, which effectively improves the crystallinity of the textured perovskite, thus suppressing the nonradiative recombination and ultimately improving the photovoltaic outputs of modified devices, particularly the fill factor and the open-circuit voltage. Thus, the photovoltaic performance of the AC-modified planar PSC is significantly better than that of the conventional textured device, with a reverse efficiency of 21.18% and forward efficiency of 20.77%. Owing to the synergistic effect of (1) the superior optical properties of the textured perovskite induced by DMF and (2) excellent charge dynamics driven by AC, the functionalized devices without encapsulation also exhibited good photovoltaic output stability and reproducibility. This work provides novel insights into the growth mechanism of textured absorbers and paves the way for more efficient and stable planar PSCs.
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http://dx.doi.org/10.1021/acsami.1c07971DOI Listing
August 2021

IL-7 and CCL19-secreting CAR-T cell therapy for tumors with positive glypican-3 or mesothelin.

J Hematol Oncol 2021 07 29;14(1):118. Epub 2021 Jul 29.

Department of Interventional Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.

Although chimeric antigen receptor (CAR)-engineered T cells have shown great success in the treatment of B cell malignancies, this strategy has limited efficacy in patients with solid tumors. In mouse CAR-T cells, IL-7 and CCL19 expression have been demonstrated to improve T cell infiltration and CAR-T cell survival in mouse tumors. Therefore, in the current study, we engineered human CAR-T cells to secrete human IL-7 and CCL19 (7 × 19) and found that these 7 × 19 CAR-T cells showed enhanced capacities of expansion and migration in vitro. Furthermore, 7 × 19 CAR-T cells showed superior tumor suppression ability compared to conventional CAR-T cells in xenografts of hepatocellular carcinoma (HCC) cell lines, primary HCC tissue samples and pancreatic carcinoma (PC) cell lines. We then initiated a phase 1 clinical trial in advanced HCC/PC/ovarian carcinoma (OC) patients with glypican-3 (GPC3) or mesothelin (MSLN) expression. In a patient with advanced HCC, anti-GPC3-7 × 19 CAR-T treatment resulted in complete tumor disappearance 30 days post intratumor injection. In a patient with advanced PC, anti-MSLN-7 × 19 CAR-T treatment resulted in almost complete tumor disappearance 240 days post-intravenous infusion. Our results demonstrated that the incorporation of 7 × 19 into CAR-T cells significantly enhanced the antitumor activity against human solid tumor. Trial registration: NCT03198546. Registered 26 June 2017, https://clinicaltrials.gov/ct2/show/NCT03198546?term=NCT03198546&draw=2&rank=1.
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http://dx.doi.org/10.1186/s13045-021-01128-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8323212PMC
July 2021

Blockade of GITRL/GITR signaling pathway attenuates house dust mite-induced allergic asthma in mice through inhibition of MAPKs and NF-κB signaling.

Mol Immunol 2021 09 20;137:238-246. Epub 2021 Jul 20.

Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing, PR China; National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University, Chongqing, PR China. Electronic address:

GITRL/GITR signaling pathway plays an important role in allergy, inflammation, transplantation and autoimmunity. However, its role in asthma remains unclear. Thus, the present study aimed to investigate changes in this pathway and observe the therapeutic effect of its blocking on asthma. By using house dust mite-induced asthma model, changes of GITRL/GITR and its downstream molecules MAPKs (e.g., p38 MAPK, JNK and Erk) and NF-κB were observed. After that, GITRL in lung of mice was knocked down by recombinant adeno-associated virus to observe the impact on its downstream molecules and assess the therapeutic effect on asthma. These results showed that GITRL/GITR and its downstream molecules MAPKs/NF-κB were activated in asthmatic mice. This activation was suppressed after GITRL knockdown, and allergic airway inflammation and airway hyperresponsiveness were alleviated. These results demonstrate that GITRL/GITR-MAPKs/NF-κB signaling pathway participates in the pathogenesis of asthma. Blockade of GITRL/GITR signaling pathway exhibits protective effects in a mouse model of house dust mite-induced allergic asthma.
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http://dx.doi.org/10.1016/j.molimm.2021.07.005DOI Listing
September 2021

BCL6B hypermethylation predicts metastasis and poor prognosis in early-stage hepatocellular carcinoma after thermal ablation.

J Cancer Res Ther 2021 Jul;17(3):644-651

Department of Interventional Ultrasound, Chinese PLA General Hospital, Beijing, China.

Aims: The aim of this study was to evaluate the role of BCL6B methylation in the progression of early-stage hepatocellular carcinoma (HCC) after thermal ablation.

Settings And Design: This is a retrospective study and written informed consent was obtained from all patients or their legal guardians.

Subjects And Methods: Between October 2008 and December 2013, 73 patients with early-stage HCC within the Milan criteria, who received thermal ablation, were recruited.

Statistical Analysis Used: Based on methylation-specific polymerase chain reaction, the relationship between BCL6B methylation and patient characteristics and prognosis was analyzed using univariate, multivariate, and Kaplan-Meier analysis.

Results: The median follow-up period was 56 (8-110) months. For the BCL6B unmethylated group, the 1-, 3- and 5-year metastasis and overall survival (OS) rates after thermal ablation were 10.0%, 10.0%, and 40.0% and 100%, 100% and 90.0%, respectively. The 1-, 3-, and 5-year metastasis and OS rates of the methylated group were 23.8%, 66.7% and 88.9% and 66.2%, 71.4% and 41.3%, respectively. Levels of absolute count lymphocyte, serum cholinesterase and albumin in the BCL6B unmethylated group were higher than those in the methylated group (P = 0.020, 0.000, and 0.009, respectively). Kaplan-Meier analysis revealed that BCL6B methylation was related to metastasis and poor prognosis (P = 0.001 and 0.018, respectively). Univariate analysis revealed that BCL6B methylation was a risk factor for metastasis and poor prognosis (odds ratio [OR]: 5.663; 95% confidence interval [CI], 1.745-18.375, P = 0.004 and OR: 3.734; 95% CI, 1.151-12.110, P = 0.028, respectively). Multivariate analysis revealed that BCL6B methylation was an independent risk factor for metastasis (OR: 3.736; 95% CI, 1.000-13.963,P = 0.05) and not for prognosis (OR: 2.780; 95% CI, 0.835-9.250,P = 0.096).

Conclusions: BCL6B methylation could be a valuable prognostic factor for metastasis and poor prognosis in early-stage HCC after thermal ablation, which is an independent risk factor for metastasis. Our findings provide insights for combining ablation and epigenetic therapy for patients with HCC.
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http://dx.doi.org/10.4103/jcrt.JCRT_1504_20DOI Listing
July 2021

Perioperative Nursing Care of Vascular Decompression for Trigeminal Neuralgia under AR Medical Technology.

J Healthc Eng 2021 19;2021:9921094. Epub 2021 Jun 19.

The First Department of Neurosurgery, People's Hospital of Rizhao, Rizhao 276800, Shandong, China.

AR technology, also known as AR or virtual reality, refers to a technology that combines and allows interaction of the virtual world on the display system with the real world through the position and angle of the camera video and image analysis technology. This technology is different from VR technology, and its characteristics can be easily explained as follows: when using AR technology, the user's eyes can see not only the real world but also the virtual world derived from the computer through things in the real world. At present, AR has been widely used in education, engineering, entertainment, and medical fields. In order to provide better perioperative care and bring patients a good nursing experience, this article mainly introduces the perioperative care of vascular decompression in the treatment of trigeminal neuralgia by augmented reality medical technology, in order to provide better care for patients with trigeminal neuralgia. This article proposes the perioperative nursing research method of vascular decompression for the treatment of trigeminal neuralgia under AR medical technology, including an overview of trigeminal neuralgia, perioperative related research, and AR medical technology algorithms, and designs related experiments to study whether AR medical technology can bring good news to nursing. Experimental results show that 96% of patients believe that with the enhancement of realistic medical technology, perioperative vascular decompression care for trigeminal neuralgia can help them recover faster and can be gradually popularized.
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http://dx.doi.org/10.1155/2021/9921094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238585PMC
June 2021

Overlapping Structures Detection in Protein-Protein Interaction Networks Using Community Detection Algorithm Based on Neighbor Clustering Coefficient.

Front Genet 2021 23;12:689515. Epub 2021 Jun 23.

Key Laboratory of Symbol Computation and Knowledge Engineering of Ministry of Education, College of Computer Science and Technology, Jilin University, Changchun, China.

With the rapid development of bioinformatics, researchers have applied community detection algorithms to detect functional modules in protein-protein interaction (PPI) networks that can predict the function of unknown proteins at the molecular level and further reveal the regularity of cell activity. Clusters in a PPI network may overlap where a protein is involved in multiple functional modules. To identify overlapping structures in protein functional modules, this paper proposes a novel overlapping community detection algorithm based on the neighboring local clustering coefficient (NLC). The contributions of the NLC algorithm are threefold: (i) Combine the edge-based community detection method with local expansion in seed selection and the local clustering coefficient of neighboring nodes to improve the accuracy of seed selection; (ii) A method of measuring the distance between edges is improved to make the result of community division more accurate; (iii) A community optimization strategy for the excessive overlapping nodes makes the overlapping structure more reasonable. The experimental results on standard networks, Lancichinetti-Fortunato-Radicchi (LFR) benchmark networks and PPI networks show that the NLC algorithm can improve the Extended modularity (EQ) value and Normalized Mutual Information (NMI) value of the community division, which verifies that the algorithm can not only detect reasonable communities but also identify overlapping structures in networks.
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http://dx.doi.org/10.3389/fgene.2021.689515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261288PMC
June 2021

Silver carp scale gelatins for the stabilization of fish oil-loaded emulsions.

Int J Biol Macromol 2021 Sep 9;186:145-154. Epub 2021 Jul 9.

National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Integrated Scientific Research Base on Comprehensive Utilization Technology for By-Products of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, Liaoning Province, China. Electronic address:

Herein, three types of silver carp scale gelatins were extracted, and their molecular weight distribution, structural properties, functional properties and emulsifying properties were investigated and discussed. Acetic acid-extracted gelatin (AAG), hot water-extracted gelatin (HWG), and pepsin enzyme-extracted gelatin (PEG) showed similar and four clear bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis pattern, whereas they showed different β chain amounts and β-sheet percentages. The water-holding capacity values (g/g of gelatin) were: AAG (16.8 ± 1.1) > HWG (14.0 ± 0.7) ≈ PEG (13.5 ± 1.6). The fat-binding capacity values (g/g of gelatin) were: AAG (11.8 ± 0.3) > HWG (9.5 ± 1.3) > PEG (5.3 ± 0.4). Emulsion droplet sizes and creaming index values decreased with the increase of gelatin concentrations for all the fish oil-loaded emulsions stabilized by three types of gelatins. Compared with PEG, AAG and HWG show similar and higher emulsion stability at high gelatin concentration (10 mg/mL). The stabilization mechanism of fish oil-loaded silver carp scale gelatin-stabilized emulsions involved an "extraction method-protein molecular weight distribution-protein molecular structure-molecular interaction-emulsibility-droplet structure-emulsion stability" route. This work would be beneficial for the research on the relationship of structure and function of gelatin and to the comprehensive utilization of aquatic products.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.043DOI Listing
September 2021

Removal of organic contaminants from wastewater with GO/MOFs composites.

PLoS One 2021 8;16(7):e0253500. Epub 2021 Jul 8.

State Key Laboratory of Advanced Design and Manufacturing for Vehicle Body, College of Mechanical and Vehicle Engineering, Hunan University, Changsha City, P.R. China.

Graphene oxide/metal-organic frameworks (GO/MOFs) have been prepared via solvothermal synthesis with ferrous sulfate heptahydrate, zirconium acetate and terephthalic acid for the purpose of removing organic pollutants from wastewater. The composites were analyzed using scanning electron microscopy, infrared spectrometry, and XRD. Tetracycline hydrochloride and orange II were implemented as model pollutants to evaluate the efficacy of the GO/MOFs in water purification, in which 50 mg of Zr/Fe-MOFs/GO was mixed with 100 mL of 10 mg/L, 20 mg/L, 30 mg/L, or 50 mg/L tetracycline hydrochloride solution and 25 mg/L, 35 mg/L, 45 mg/L, or 60 mg/L orange II solution, respectively. The removal efficacy after 4 hours was determined to be 96.1%, 75.8%, 55.4%, and 30.1%, and 98.8%, 91.9%, 71.1%, and 66.2%, respectively. The kinetics of pollutant removal was investigated for both tetracycline hydrochloride and orange II and excellent correlation coefficients of greater than 0.99 were obtained. The high efficacy of these MOFs in pollutant removal, coupled with their inexpensive preparation indicates the feasibility of their implementation in strategies for treating waste liquid. As such, it is anticipated that Zr/Fe-MOFs/GO composites will be widely applied in wastewater purification.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253500PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266086PMC
July 2021

Genotype-Phenotype Correlations in Angelman Syndrome.

Genes (Basel) 2021 06 28;12(7). Epub 2021 Jun 28.

Department of Endocrinology, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou 310052, China.

Angelman syndrome (AS) is a rare neurodevelopmental disease that is caused by the loss of function of the maternal copy of ubiquitin-protein ligase E3A () on the chromosome 15q11-13 region. AS is characterized by global developmental delay, severe intellectual disability, lack of speech, happy disposition, ataxia, epilepsy, and distinct behavioral profile. There are four molecular mechanisms of etiology: maternal deletion of chromosome 15q11-q13, paternal uniparental disomy of chromosome 15q11-q13, imprinting defects, and maternally inherited UBE3A mutations. Different genetic types may show different phenotypes in performance, seizure, behavior, sleep, and other aspects. AS caused by maternal deletion of 15q11-13 appears to have worse development, cognitive skills, albinism, ataxia, and more autistic features than those of other genotypes. Children with a mutation have less severe phenotypes and a nearly normal development quotient. In this review, we proposed to review genotype-phenotype correlations based on different genotypes. Understanding the pathophysiology of the different genotypes and the genotype-phenotype correlations will offer an opportunity for individualized treatment and genetic counseling. Genotype-phenotype correlations based on larger data should be carried out for identifying new treatment modalities.
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http://dx.doi.org/10.3390/genes12070987DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304328PMC
June 2021

Sociodemographic factors associated with the first administration of anti-seizure medication in patients with focal epilepsy in Western China.

BMC Neurol 2021 Jun 29;21(1):251. Epub 2021 Jun 29.

Department of Neurology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China.

Background: Epilepsy is a severe chronic neurologic disease with a prevalence of 0.7% worldwide; anti-seizure medications (ASMs) are the mainstay of epilepsy treatment. The effects of sociodemographic factors on the characteristics of initial treatment in patients with newly diagnosed focal epilepsy in Western China are unknown. This study was conducted to explore sociodemographic factors associated with initial treatment characteristics.

Methods: Patients with focal epilepsy on continuous ASM treatment who visited to our epilepsy center at Sichuan Provincial People's Hospital between January 2018 and December 2019 were recruited. Data on initial treatment status and sociodemographic variables were obtained from the patients with a questionnaire designed by our researchers. We examined whether sociodemographic factors were associated with epileptic patients' access to neurologists and prescriptions of individual ASMs.

Results: A total of 569 patients completed this study. We found that patients with a higher education level, aged < 16 years, and with a higher household disposable income were more likely to receive treatment from a neurologist than their counterparts. Patients with a lower personal income level and who were treated at a junior hospital were more likely to receive prescriptions for carbamazepine, and those who were younger than 16 years were less likely to receive prescriptions for carbamazepine and oxcarbazepine. Patients with a higher education level, with a higher household disposable income level, who were younger than 16 years, and who were treated at a senior hospital were more likely to receive prescriptions for levetiracetam than their counterparts. Adult, female patients with focal epilepsy treated at a senior hospital were more likely to receive prescriptions for lamotrigine.

Conclusions: This observation suggests that sociodemographic characteristics are associated with access to neurologists and prescriptions of individual antiepileptic drugs. These data may help public health officials establish guidelines for doctors and distribute resources according to the needs of different patient groups.
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http://dx.doi.org/10.1186/s12883-021-02282-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240397PMC
June 2021

Gender Differences in Transnational Brand Purchase Decision Toward Mixed Culture and Original Culture Advertisements: An fNIRS Study.

Front Psychol 2021 15;12:654360. Epub 2021 Jun 15.

Shenzhen Key Laboratory of Affective and Social Neuroscience, Center for Brain Disorders and Cognitive Sciences, Shenzhen University, Shenzhen, China.

Culture strategy is very important for transnational brand marketing. Functional near-infrared spectroscopy (fNIRS) is a promising brain imaging modality for neuromarketing research. In the present study, we used fNIRS to explore the neural correlates of consumers' purchase decision on different cross-culture marketing strategies. Forty Chinese participants watched transnational brands and products advertised with photographs of the brands' original culture (the original culture advertisements) and advertised with photographs of Chinese culture (the mixed culture advertisements), respectively. The behavioral results showed that the female participants showed significantly higher purchase rate when watching the original culture advertisements than the mixed culture advertisements, whereas the male participants did not show significant preference between these two types. The fNIRS results further revealed that for the female participants, watching mixed culture advertisements evoked significant positive activation in the left dorsolateral prefrontal cortex and negative activation in the medial prefrontal cortex, which was not found in the male participants. These findings suggest possible cognitive and emotional differences between men and women in purchase decision making toward different cross-culture marketing strategy. The present study also demonstrates the great potential of fNIRS in neuromarketing research.
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http://dx.doi.org/10.3389/fpsyg.2021.654360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226242PMC
June 2021

Profiles, spatial distributions and inventory of brominated dioxin and furan emissions from secondary nonferrous smelting industries in China.

J Hazard Mater 2021 10 17;419:126415. Epub 2021 Jun 17.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China; School of Environment, Hangzhou Institute for Advanced Study, UCAS, Hangzhou 310000, China. Electronic address:

Nonferrous metallurgical processes are important sources of carcinogenic polybrominated dibenzo-p-dioxin and dibenzofuran (PBDD/Fs) that transport globally. Studies on the profiles, spatial distributions and inventory of PBDD/F emissions into the atmosphere from nonferrous metallurgical plants are needed for better source control. In this study, field investigations on PBDD/F emissions from typical nonferrous metallurgical plants were conducted to characterize the PBDD/F profiles and derive their emission factors. Based on the PBDD/F profiles, diagnostic ratios of PBDD/Fs for secondary copper, zinc and lead smelting were proposed for identifying the potential sources of PBDD/Fs in environment. The PBDD/F emission factors for the secondary copper, lead, and zinc smelting plants were 0.71, 1.65, and 1.54 μg toxic equivalents/t, respectively. The estimated annual input of PBDD/Fs into atmosphere by secondary nonferrous metallurgical plants in China was 212.4 g by mass and 3511.3 mg by toxic equivalents, which is of significance for further evolving a global inventory. The spatial distribution of PBDD/F emissions from nonferrous metallurgical plants in China was mapped. Larger amounts of PBDD/Fs were emitted in the southeastern coastal region and northern China than elsewhere in China.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126415DOI Listing
October 2021

Safety and efficacy of S1 monotherapy or combined with nab-paclitaxel in advanced elderly pancreatic cancer patients: A meta-analysis.

Medicine (Baltimore) 2021 Jun;100(25):e26342

Department of Hepatobiliary Surgery, the First Affiliated Hospital of Dalian Medical University.

Objective: To evaluate the therapeutic efficacy and safety of S1 monotherapy or combination with nab-paclitaxel for the treatment of elderly patients with metastatic or locally advanced pancreatic adenocarcinoma.

Method: PubMed, Embase, Cochrane Central Library, China Biology Medicine, and China National Knowledge Infrastructure databases were searched without time limits according to the inclusion criteria. RevMan (Version 5.3) software was used for data extraction and meta-analysis. Objective response rate (ORR) and disease control rate (DCR) were used to evaluate therapeutic effects while side effects including leukopenia, thrombocytopenia, neurotoxicity, vomit, and alopecia were extracted for evaluation. There was no need for ethical review in this study because no ethical experiments were conducted and all data used were public data. All relevant data are within the paper and its Supporting Information files.

Results: Four retrospective studies comprising 308 elderly patients with metastatic or locally advanced pancreatic adenocarcinoma were included in the analysis. One hundred fifty-one patients underwent S1 monotherapy and 157 received S1 combined nab-paclitaxel. Meta-analysis indicated that compared with S1 monotherapy, S1 combined with nab-paclitaxel had higher ORR (OR 2.25, 95% CI: 1.42-3.55; P = .0005) and DCR (OR 2.94, 95% CI: 1.55-5.58; P = .0009). The adverse reaction of leukopenia was higher in the combined therapy group (OR 1.85, 95% CI: 1.09-3.13, P = .02), but no significant difference was found in thrombocytopenia, neurotoxicity, vomiting, and alopecia between the 2 groups (P > .05).

Conclusion: Nab-paclitaxel plus S1 was more efficient in terms of ORR and DCR than S1 monotherapy in elderly pancreatic ductal adenocarcinoma patients while the side effect was controllable with a higher probability of leukopenia. Thus, combined nab-paclitaxel and S1 could be safely used in elderly patients.
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http://dx.doi.org/10.1097/MD.0000000000026342DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238291PMC
June 2021
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