Publications by authors named "Lili Lu"

139 Publications

[Non-coding RNAs in viral myocarditis].

Sheng Wu Gong Cheng Xue Bao 2021 Sep;37(9):3101-3107

Institute of Pharmaceutical Innovation, College of Medicine, Wuhan University of Science and Technology, Wuhan 430065, Hubei, China.

Viral myocarditis (VMC) is a disease characterized by inflammation of myocardial cells caused by viral infection. Since the pathogenesis mechanism of VMC has not been fully elucidated, the diagnosis and treatment of this disease remains extremely challenging. Non-coding RNAs (ncRNAs) are a class of RNAs that do not encode proteins. An increasing number of studies have shown that ncRNAs are involved in regulating the occurrence and development of VMC, thus providing potential new targets for the treatment and diagnosis of VMC. This review summarizes the possible roles of ncRNAs in the pathogenesis and diagnosis of VMC revealed recently.
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http://dx.doi.org/10.13345/j.cjb.200698DOI Listing
September 2021

Genome-wide analysis of ZAT gene family revealed GhZAT6 regulates salt stress tolerance in G. hirsutum.

Plant Sci 2021 Nov 10;312:111055. Epub 2021 Sep 10.

Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China; State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China. Electronic address:

High salt environments can induce stress in different plants. The genes containing the ZAT domain constitute a family that belongs to a branch of the CH family, which plays a vital role in responding to abiotic stresses. In this study, we identified 169 ZAT genes from seven plant species, including 44 ZAT genes from G. hirsutum. Phylogenetic tree analysis divided ZAT genes in six groups with conserved gene structure, protein motifs. Two CH domains and an EAR domain and even chromosomal distribution on At and Dt sub-genome chromosomes of G. hirsutum was observed. GhZAT6 was primarily expressed in the root tissue and responded to NaCl and ABA treatments. Subcellular localization found that GhZAT6 was located in the nucleus and demonstrated transactivation activity during a transactivation activity assay. Arabidopsis transgenic lines overexpressing the GhZAT6 gene showed salt tolerance and grew more vigorously than WT on MS medium supplemented with 100 mmol NaCl. Additionally, the silencing of the GhZAT6 gene in cotton plants showed more obvious leaf wilting than the control plants, which were subjected to 400 mmol NaCl treatment. Next, the expressions of GhAPX1, GhFSD1, GhFSD2, and GhSOS3 were significantly lower in the GhZAT6-silenced plants treated with NaCl than the control. Based on these findings, GhZAT6 may be involved in the ABA pathway and mediate salt stress tolerance by regulating ROS-related gene expression.
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http://dx.doi.org/10.1016/j.plantsci.2021.111055DOI Listing
November 2021

Identification and Analysis of Gene Family Indicated That Promotes Plant Growth and Development Through Brassinosteroid Signaling in Cotton ( L.).

Front Plant Sci 2021 16;12:719889. Epub 2021 Sep 16.

Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, China.

Brassinosteroids (BRs), an efficient plant endogenous hormone, significantly promotes plant nutrient growth adapting to biological and abiotic adversities. BRs mainly promote plant cell elongation by regulating gene expression patterns. () genes have been characterized as the indicators of BR response genes. Cotton, an ancient crop, is of great economic value and its fibers can be made into all kinds of fabrics. However, gene family genes have not been full identified in cotton. 175 genes were identified in nine plant species, of which 39 genes in in our study. A phylogenetic analysis grouped all of the proteins encoded by the genes into five major clades. Sequence identification of conserved amino acid residues among monocotyledonous and dicotyledonous species showed a high level of conservation across the N and C terminal regions. Only 25% the genes contain introns besides conserved gene structure and protein motifs distribution. The 39 genes were unevenly distributed on the 18 At and Dt sub-genome chromosomes. Most of the genes were derived from gene duplication events, while only three genes showed evidence of tandem duplication. Homologous locus relationships showed that 15 genes are located on collinear blocks and that all orthologous/paralogous gene pairs had > values, indicating purifying selection pressure. The genes showed ubiquitous expression in all eight tested cotton tissues and following exposure to three phytohormones, IAA, GA, and BL. Furthermore, was mainly expressed in response to BL treatment, and was predominantly expressed in the fibers. was found to be a plasma membrane protein, and its ectopic expression in mediated BR-regulated plant growth and development with altered expression of , , , and . Additionally, the functions of were confirmed by virus-induced gene silencing (VIGS) in cotton. This study will provide important genetic resources for future cotton breeding programs.
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http://dx.doi.org/10.3389/fpls.2021.719889DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481617PMC
September 2021

TAM-derived extracellular vesicles containing microRNA-29a-3p explain the deterioration of ovarian cancer.

Mol Ther Nucleic Acids 2021 Sep 19;25:468-482. Epub 2021 May 19.

Department of Obstetrics and Gynecology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, No. 639, Zhizaoju Road, Huangpu District, Shanghai 200011, PRC China.

Extracellular vesicles (EVs) secreted from tumor-associated macrophages (TAMs) are known to generate an immune-suppressive environment conducive to the development of ovarian cancer (OC). We tried to elucidate the role of TAM-derived exosomal microRNA (miR)-29a-3p in OC. miR-29a-3p, forkhead box protein O3 (FOXO3), and programmed death ligand-1 (PD-L1) expression was determined and their interactions evaluated. EVs were isolated, followed by determination of the uptake of EVs by OC cells, after which the proliferation and immune escape facilities of the OC cells were determined. OC xenograft models were constructed with EVs in correspondence with experiments. Overexpressed miR-29a-3p was detected in OC, and miR-29a-3p promoted OC cell proliferation and immune escape. EVs derived from TAMs enhanced the proliferation of OC cells. miR-29a-3p was enriched in TAM-EVs, and TAM-EVs delivered miR-29a-3p into OC cells. Downregulated FOXO3 was identified in OC, whereas miR-29a-3p targeted FOXO3 to suppress glycogen synthase kinase 3β (GSK3β) activity via the serine/threonine protein kinase (AKT)/GSK3β pathway. Inhibition of TAM-derived exosomal miR-29a-3p decreased PD-L1 to inhibit OC progression through the FOXO3-AKT/GSK3β pathway and . Taken together, the current studies highlight the FOXO3-AKT/GSK3β pathway and the mechanism by which TAM-derived exosomal miR-29a-3p enhances the expression of PD-L1 to facilitate OC cell proliferation and immune escape.
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http://dx.doi.org/10.1016/j.omtn.2021.05.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463289PMC
September 2021

A global assessment of recent trends in gastrointestinal cancer and lifestyle-associated risk factors.

Cancer Commun (Lond) 2021 Sep 25. Epub 2021 Sep 25.

Department of General Surgery, Molecular Oncology and Immunotherapy, Rostock University Medical Center, Rostock, 18057, Germany.

Background: Gastrointestinal (GI) cancers were responsible for 26.3% of cancer cases and 35.4% of deaths worldwide in 2018. This study aimed to analyze the global incidence, mortality, prevalence, and contributing risk factors of the 6 major GI cancer entities [esophageal cancer (EC), gastric cancer (GC), liver cancer (LC), pancreatic cancer (PC), colon cancer, and rectal cancer].

Methods: Using the Global Cancer Observatory and the Global Health Observatory databases, we reviewed the current GI cancer incidence, prevalence, and mortality, analyzed the association of GI cancer prevalence with national human development indices (HDIs), identified the contributing risk factors, and estimated developing age- and sex-specific trends in incidence and mortality.

Results: In 2020, the trend in age-standardized rate of incidence of GI cancers closely mirrored that of mortality, with the highest rates of LC, EC, and GC in Asia and of colorectal cancer (CRC) and PC mainly in Europe. Incidence and mortality were positively, but the mortality-to-incidence ratio (MIR) was inversely correlated with the national HDI levels. High MIRs in developing countries likely reflected the lack of preventive strategies and effective treatments. GI cancer prevalence was highest in Europe and was also positively correlated with HDIs and lifestyle-associated risk factors, such as alcohol consumption, smoking, obesity, insufficient physical activity, and high blood cholesterol level, but negatively correlated with hypertension and diabetes. Incidences of EC were consistently and those of GC mostly decreasing, whereas incidences of CRC were increasing in most countries/regions, especially in the younger populations. Incidences of LC and PC were also increasing in all age-gender populations except for younger males. Mortalities were decreasing for EC, GC, and CRC in most countries/regions, and age-specific trends were observed in PC and LC with a decrease in the younger but an increase in the older population.

Conclusions: On the global scale, higher GI cancer burden was accompanied, for the most part, by factors associated with the so-called Western lifestyle reflected by high and very high national HDI levels. In countries/regions with very high HDI levels, patients survived longer, and increasing GI cancer cases were observed with increasing national HDI levels. Optimizing GI cancer prevention and improving therapies, especially for patients with comorbid metabolic diseases, are thus urgently recommended.
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http://dx.doi.org/10.1002/cac2.12220DOI Listing
September 2021

Identification of BR biosynthesis genes in cotton reveals that GhCPD-3 restores BR biosynthesis and mediates plant growth and development.

Planta 2021 Sep 17;254(4):75. Epub 2021 Sep 17.

Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.

Main Conclusion: Brassinosteroid (BR) synthesis genes in different cotton species was comprehensively identified, and the participation of GhCPD-3 in the BR synthesis signaling pathway for regulating plant development was verified. Brassinosteroid is a natural steroidal phytohormone that plays fundamental roles in plant growth and development. In cotton, detailed characterization and functional validation of BR biosynthesis genes remain rare. Here, 16, 8 and 9 BR biosynthesis genes were identified in Gossypium hirsutum, Gossypium raimondii and Gossypium arboreum, respectively, and their phylogenetic relationships, gene structures, conserved motifs of the encoded proteins, chromosomal locations were determined and a synteny analysis was performed. Gossypium hirsutum and Arabidopsis BR biosynthesis genes closely clustered in the phylogenetic tree and fragment duplication was likely the primary cause promoting gene family expansion in G. hirsutum. Gene Ontology (GO) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis showed their relevance as BR biosynthesis genes. GhCPD-3 was highly expressed in roots and stems and the loci of single nucleotide polymorphisms (SNPs) were significantly associated with these traits.Ectopic overexpression of GhCPD-3 in the cpd91 Arabidopsis mutant rescued the mutant phenotype by increasing plant height and leaf size in comparison to those of cpd91 and WT plants. Moreover, overexpressed GhCPD-3 in cpd91 mutants showed greater hypocotyl and root lengths than those of cpd91 and WT plants under light and dark conditions, respectively, indicating that BR actively promotes hypocotyl and root growth. Similar to CPD (CONSTITUTIVE PHOTOMORPHOGENIC DWARF), GhCPD-3 restores BR biosynthesis thereby mediating plant growth and development.
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http://dx.doi.org/10.1007/s00425-021-03727-9DOI Listing
September 2021

Identification and Characterization of the ERF Subfamily B3 Group Revealed Improves Salt Tolerance in Upland Cotton.

Front Plant Sci 2021 9;12:705883. Epub 2021 Aug 9.

State Key Laboratory of Cotton Biology, Zhengzhou Research Base, Zhengzhou University, Zhengzhou, China.

The APETALA2 (AP2)/ethylene response factor plays vital functions in response to environmental stimulus. The ethylene response factor (ERF) subfamily B3 group belongs to the AP2/ERF superfamily and contains a single AP2/ERF domain. Phylogenetic analysis of the ERF subfamily B3 group genes from , and made it possible to divide them into three groups and showed that the ERF subfamily B3 group genes are conserved in cotton. Collinearity analysis identified172 orthologous/paralogous gene pairs between and ; 178 between and ; and 1,392 in . The subfamily B3 group gene family experienced massive gene family expansion through either segmental or whole genome duplication events, with most genes showing signature compatible with the action of purifying selection during evolution. Most ERF subfamily B3 group genes are responsive to salt stress. transgenic Arabidopsis showed enhanced salt stress tolerance and exhibited regulation of related biochemical parameters and enhanced expression of genes participating in ABA signaling, proline biosynthesis, and ROS scavenging. In addition, the silencing of the gene leads to increased sensitivity to salt stress in cotton. These results indicate that the ERF subfamily B3 group had remained conserved during evolution and that induces salt stress tolerance in Arabidopsis and cotton.
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http://dx.doi.org/10.3389/fpls.2021.705883DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382128PMC
August 2021

Reactive oxygen species induced by uric acid promote NRK‑52E cell apoptosis through the NEK7‑NLRP3 signaling pathway.

Mol Med Rep 2021 Oct 20;24(4). Epub 2021 Aug 20.

Department of Nephrology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, TCM Institute of Kidney Disease, Shanghai University of Traditional Chinese Medicine, Key Laboratory of Liver and Kidney Diseases (Shanghai University of Traditional Chinese Medicine), Ministry of Education, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai 201203, P.R. China.

Increasing uric acid (UA) could induce renal tubular epithelial cell (NRK‑52E) injury. However, the specific mechanism by which UA induces renal tubular epithelial cell injury remains unknown. It was hypothesized that UA induces renal tubular epithelial cell injury through reactive oxygen species (ROS) and the Never in mitosis gene A (NIMA)‑related kinase 7 (NEK7)/NLR family pyrin domain containing 3 (NLRP3) signaling pathway. TUNEL assay and flow cytometry were applied to measure apoptosis, and the results of the present study showed that UA treatment induced apoptosis of NRK‑52E cells in a concentration‑dependent manner. Western blotting was performed to determine the expression levels of cleaved caspase‑3, Bax and Bcl‑xl, it was found that levels were significantly increased after UA treatment in NRK‑52E cells. ROS and apoptosis were predominantly induced in NRK‑52E cells and there was an association between ROS and apoptosis. Enhanced expression of NEK7, NLRP3, apoptosis‑associated speck‑like and caspase‑1 were observed in NRK‑52E cells treated with UA. The ROS inhibitor, N‑acetyl‑l‑cysteine, exerted a protective effect on the UA‑induced apoptosis of tubular epithelial cells by reducing excess ROS production, which significantly inhibited NEK7 and NLRP3 inflammasome activation. These results indicated that UA activates ROS and induces apoptosis of NRK‑52E cells. The mechanism might be related to the regulation of the NEK7/NLRP3 signaling pathway.
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http://dx.doi.org/10.3892/mmr.2021.12368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383041PMC
October 2021

Influence of bovine serum albumin on corrosion behaviour of pure Zn in phosphate buffered saline.

J Mater Sci Mater Med 2021 Aug 18;32(9):95. Epub 2021 Aug 18.

Beijing Advanced Innovation Centre for Materials Genome Engineering, School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, China.

Zinc (Zn) and its alloys have received increasing attention as new alternative biodegradable metals. However, consensus has not been reached on the corrosion behaviour of Zn. As cardiovascular artery stent material, Zn is supposed to contact with plasma that contains inorganic salts and organic components. Protein is one of the most important constitute in the plasma and could adsorb on the material surface. In this paper, bovine serum albumin (BSA) was used as a typical protein. Influences of BSA on pure Zn corrosion in phosphate buffered saline is investigated as a function of BSA concentrations and immersion durations by electrochemical techniques and surface analysis. Results showed that pure Zn corrosion was progressively accelerated with BSA concentrations (ranging from 0.05 to 5 g L) at 0.5 h. With time evolves, formation of phosphates as corrosion product was delayed by BSA adsorption, especially at concentration of 2 g L. Within 48 h, the corrosion of pure Zn was alleviated by BSA at concentration of 0.1 g L, whereas the corrosion was enhanced after 168 h. Addition of 2 g L BSA has opposite influence on the pure Zn corrosion. Furthermore, schematic corrosion behaviour at protein/Zn interfaces was proposed. This work encourages us to think more about the influence of protein on the material corrosion and helps us to better understand the corrosion behaviour of pure Zn.
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http://dx.doi.org/10.1007/s10856-021-06567-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373726PMC
August 2021

Controlled preparation of hollow ZnCdS nanospheres modified by NiS nanosheets for superior photocatalytic hydrogen production.

J Colloid Interface Sci 2021 Aug 4;606(Pt 1):1-9. Epub 2021 Aug 4.

State Key Laboratory for Powder Metallurgy, Central South University, Changsha 410083, PR China. Electronic address:

Developing durable and efficient photocatalysts for H evolution is highly desirable to expedite current research on solar-chemical energy conversion. In this work, a novel photocatalytic H evolution system based on ZnCdS/NiS nanocomposite was rationally designed for the first time. In this advanced composite structure, NiS nanosheets as a co-catalyst were intimately coupled to the inner surface of the hollow spherical ZnCdS. The construction of the hollow spherical shell shortened the distance of charge migration to the surface site and increased the multiple absorption of incident light. The introduction of NiS nanosheets increased the light absorption capacity of the composite system and also greatly improved the separation and migration behavior of photo-generated carriers due to its narrower band gap and relatively low conduction band position, which had been confirmed by DRS, EIS and PL. As a result, the hollow ZnCdS/NiS composite material exhibited excellent photocatalytic activity. At the loading amount of NiS up to 15 at.%, the hollow ZnCdS/NiS composite exhibited the best photocatalytic activity with a corresponding H production rate of 22.637 mmol gh, which was 1.42 times and 1.85 times that of hollow ZnCdS and solid ZnCdS, respectively. Moreover, this novel catalyst also displayed a long-term stability without apparent debasement in H evolution activity. It is expected that this work could provide new inspiration to the design and development of other highly active photocatalytic systems for water splitting.
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http://dx.doi.org/10.1016/j.jcis.2021.08.006DOI Listing
August 2021

Overexpression of let-7d explains down-regulated KDM3A and ENO2 in the pathogenesis of preeclampsia.

J Cell Mol Med 2021 Sep 5;25(17):8127-8139. Epub 2021 Aug 5.

Department of Obstetrics, Linyi People's Hospital, Linyi, China.

Pre-eclampsia (PE) is the leading cause of maternal death; however, the causative molecular basis remains largely unknown. Recent studies have revealed the important role microRNAs (miRNAs) play in PE. We aimed to explore the effects of let-7d on trophoblast proliferation, migration, invasion and apoptosis in PE and its underlying mechanism. Placental tissues were collected from PE patients and healthy pregnant women, and it was found that let-7d expression was increased, while KDM3A and ENO2 expression was decreased in PE tissues and cells. Bioinformatics analysis indicated the interaction among let-7d, KDM3A and ENO2, confirmed by dual luciferase reporter gene assay; ChIP experiment identified methylated modification to ENO2 by KDM3A. With gain- and loss-function method, silencing of let-7d increased KDM3A expression and enhanced the binding between KDM3A and ENO2. Furthermore, overexpression of let-7d suppressed cell proliferation, migration and invasion of trophoblasts, and induced apoptosis of trophoblasts, while these capacities were restored upon additional treatment of overexpressed ENO2. PE rat models were established to explore the effects of let-7d and ENO2 on PE in vivo. The results established that the silencing of let-7d alleviated the tissue injury and PE-related symptoms when reducing urine protein, TUNEL-positive cells and increasing ENO2, and KDM3A expression in rats. Cumulatively, let-7d suppressed cell progression of trophoblasts, and induced apoptosis through the down-regulation of KDM3A to promote ENO2 methylation, thereby promoting progression of PE. Such an epigenetic network of let-7d, KDM3A and ENO2 in the pathogenesis of PE might provide novel insight into targeted therapy against this disorder.
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http://dx.doi.org/10.1111/jcmm.16299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419194PMC
September 2021

Long Non-Coding RNA LINC01410 Promoted Tumor Progression the ErbB Signaling Pathway by Targeting STAT5 in Gallbladder Cancer.

Front Oncol 2021 12;11:659123. Epub 2021 Jul 12.

Biotherapy Centre, Zhongshan Hospital, Fudan University, Shanghai, China.

Objectives: Long non-coding RNAs (lncRNAs) have been recently emerging as crucial molecules in multiple human cancers. However, their expression patterns, roles as well as the underlying mechanisms in gallbladder cancer (GBC) remain largely unclear.

Materials And Methods: The expression of lncRNAs in GBC was downloaded from GEO database. Quantitative real-time polymerase chain reaction (qRT-PCR) and RNA hybridization (ISH) were used to detect the expression of lncRNAs in GBC tissues. The full-sequence of LINC01410 was determined by RACE assay. Subcellular distribution of LINC01410 was examined by nuclear/cytoplasmic RNA fractionation analysis. Loss- and gain-of-function experiments were conducted to explore the biological functions of LINC01410 and . RNA pull-down, RNA immune-precipitation (RIP), and Western blot assay were conducted to investigate the mechanisms underlying the biological function of LINC01410 in GBC.

Results: LINC01410 was significantly upregulated in the GBC tissues compared to adjacent non-tumor tissues. High LINC01410 expression was significantly associated with poor prognosis of GBC patients. We identified LINC01410 to be 2,877 bp in length and mainly localized in the cytoplasm of GBC cells. Overexpression of LINC01410 promoted GBC cell proliferation, migration, and invasion and GBC progression , whereas LINC01410 downregulation rescued these effects . From RNA pull-down and RIP assay, we identified that STAT5 was a critical downstream target of LINC01410. Furthermore, ErbB signaling pathway was involved in the malignant phenotypes of GBC mediated by LINC01410.

Conclusions: Our results suggested that LINC01410 was an important lncRNA that promoted GBC progression targeting STAT5 and activating ErbB signaling pathway.
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http://dx.doi.org/10.3389/fonc.2021.659123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312242PMC
July 2021

Extracellular matrix protein-1 secretory isoform promotes ovarian cancer through increasing alternative mRNA splicing and stemness.

Nat Commun 2021 07 9;12(1):4230. Epub 2021 Jul 9.

Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai, China.

Extracellular matrix protein-1 (ECM1) promotes tumorigenesis in multiple organs but the mechanisms associated to ECM1 isoform subtypes have yet to be clarified. We report in this study that the secretory ECM1a isoform induces tumorigenesis through the GPR motif binding to integrin αXβ2 and the activation of AKT/FAK/Rho/cytoskeleton signaling. The ATP binding cassette subfamily G member 1 (ABCG1) transduces the ECM1a-integrin αXβ2 interactive signaling to facilitate the phosphorylation of AKT/FAK/Rho/cytoskeletal molecules and to confer cancer cell cisplatin resistance through up-regulation of the CD326-mediated cell stemness. On the contrary, the non-secretory ECM1b isoform binds myosin and blocks its phosphorylation, impairing cytoskeleton-mediated signaling and tumorigenesis. Moreover, ECM1a induces the expression of the heterogeneous nuclear ribonucleoprotein L like (hnRNPLL) protein to favor the alternative mRNA splicing generating ECM1a. ECM1a, αXβ2, ABCG1 and hnRNPLL higher expression associates with poor survival, while ECM1b higher expression associates with good survival. These results highlight ECM1a, integrin αXβ2, hnRNPLL and ABCG1 as potential targets for treating cancers associated with ECM1-activated signaling.
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http://dx.doi.org/10.1038/s41467-021-24315-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270969PMC
July 2021

Extracellular matrix protein-1 secretory isoform promotes ovarian cancer through increasing alternative mRNA splicing and stemness.

Nat Commun 2021 07 9;12(1):4230. Epub 2021 Jul 9.

Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai, China.

Extracellular matrix protein-1 (ECM1) promotes tumorigenesis in multiple organs but the mechanisms associated to ECM1 isoform subtypes have yet to be clarified. We report in this study that the secretory ECM1a isoform induces tumorigenesis through the GPR motif binding to integrin αXβ2 and the activation of AKT/FAK/Rho/cytoskeleton signaling. The ATP binding cassette subfamily G member 1 (ABCG1) transduces the ECM1a-integrin αXβ2 interactive signaling to facilitate the phosphorylation of AKT/FAK/Rho/cytoskeletal molecules and to confer cancer cell cisplatin resistance through up-regulation of the CD326-mediated cell stemness. On the contrary, the non-secretory ECM1b isoform binds myosin and blocks its phosphorylation, impairing cytoskeleton-mediated signaling and tumorigenesis. Moreover, ECM1a induces the expression of the heterogeneous nuclear ribonucleoprotein L like (hnRNPLL) protein to favor the alternative mRNA splicing generating ECM1a. ECM1a, αXβ2, ABCG1 and hnRNPLL higher expression associates with poor survival, while ECM1b higher expression associates with good survival. These results highlight ECM1a, integrin αXβ2, hnRNPLL and ABCG1 as potential targets for treating cancers associated with ECM1-activated signaling.
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http://dx.doi.org/10.1038/s41467-021-24315-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270969PMC
July 2021

Identification of GhLOG gene family revealed that GhLOG3 is involved in regulating salinity tolerance in cotton (Gossypium hirsutum L.).

Plant Physiol Biochem 2021 Sep 10;166:328-340. Epub 2021 Jun 10.

Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou, 450001, Henan, China. Electronic address:

Cytokinin (CK) is an important plant hormone that promotes plant cell division and differentiation, and participates in salt response under osmotic stress. LOGs (LONELY GUY) are CK-activating enzymes involved in CK synthesis. The LOG gene family has not been comprehensively characterized in cotton. In this study we identified 151 LOG genes from nine plant species, including 28 LOG genes in Gossypium hirsutum. Phylogenetic analysis divided LOG genes into three groups. Exon/intron structures and protein motifs of GhLOG genes were highly conserved. Synteny analysis revealed that several gene loci were highly conserved between the A and D sub-genomes of G. hirsutum with purifying selection pressure during evolution. Expression profiles showed that most LOG genes were constitutively expressed in eight different tissues. Furthermore, LOG genes can be regulated by abiotic stresses and phytohormone treatments. Moreover, subcellular localization revealed that GhLOG3_At resides inside the cell membrane. Overexpression of GhLOG3 enhanced salt tolerance in Arabidopsis. Virus-induced gene silencing (VIGS) of GhLOG3_At in cotton enhanced sensitivity of plants to salt stress with increased HO contents and decreased chlorophyll and proline (PRO) activity. Our results suggested that GhLOG3_At induces salt stress tolerance in cotton, and provides a basis for the use of CK synthesis genes to regulate cotton growth and stress resistance.
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http://dx.doi.org/10.1016/j.plaphy.2021.06.011DOI Listing
September 2021

Metabolic scaling: individual versus intraspecific scaling of Nile tilapia (Oreochromis niloticus).

J Comp Physiol B 2021 07 2;191(4):721-729. Epub 2021 May 2.

Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Ocean College, Beibu Gulf University, Qinzhou, China.

We examined intraspecific scaling of the resting metabolic rate (RMR) of Nile tilapia (Oreochromis niloticus) under different culture conditions and further explored the allometric relationships between organ mass (heart, liver, brain, gills, viscera, and red muscles) and blood parameters (erythrocyte size and red blood cell counts) and body mass. Oreochromis niloticus were bred in individual and group cultures. The scaling exponent of the RMR in the individual cultures was b = 0.620-0.821 (n = 30) and that in the group culture was b = 0.770 [natural logarithm (ln) RMR = 0.770 ln M - 1.107 (n = 76)]. The results of the two experimental methods were similar and were not significantly different from 0.75 (3/4), as predicted by the metabolic theory of ecology. The active and inactive organs were scaled with body mass by an exponent of 0.940 and 1.012, respectively. There was no significant relationship between the blood parameters and body mass. These results suggest that the differences in the culture methods may not have affected the allometric scaling of O. niloticus metabolism. The proportion of active and inactive organs contributed to allometric changes in the metabolic rate with body mass. Red blood cells in fish are not generally representative, and cell size can only partially explain the allometric scaling of metabolism.
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http://dx.doi.org/10.1007/s00360-021-01376-8DOI Listing
July 2021

How workplace violence correlates turnover intention among Chinese health care workers in COVID-19 context: The mediating role of perceived social support and mental health.

J Nurs Manag 2021 Apr 1. Epub 2021 Apr 1.

School of Public Health, Xuzhou Medical University, Xuzhou, China.

Aim: To elucidate the effects of workplace violence on turnover intention among Chinese health care workers, and to identify the potential mediators in this relationship.

Background: Workplace violence has emerged as a crucial determinant of turnover intention for health care workers.

Methods: A cross-sectional survey was conducted among Chinese health care workers (N = 1,063) between 13 and 20 February 2020. Mediation effects were tested using structural equation modelling with weighted least squares mean and variance adjusted (WLSMV) estimator.

Results: Workplace violence had both direct and indirect effects on turnover intention among Chinese health care workers. Specifically, perceived social support, mental health and perceived social support together with mental health partially mediated the relationship between workplace violence and turnover intention.

Conclusions: Chinese health care workers experiencing violence during the COVID-19 outbreak were more likely to report turnover intention. Enhancing social support and reducing mental health problems would be beneficial in decreasing the detrimental effects of workplace violence on turnover intention.

Implications For Nursing Management: Ensuring health care workers' health and safety is vital in reducing turnover intention, which in turn ascertains continuity of health care delivery. Health care managers should develop targeted interventions to improve social support and prevent post-violence mental health problems.
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http://dx.doi.org/10.1111/jonm.13325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250348PMC
April 2021

AMD1 upregulates hepatocellular carcinoma cells stemness by FTO mediated mRNA demethylation.

Clin Transl Med 2021 03;11(3):e352

Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, China.

Background: S-adenosylmethionine decarboxylase proenzyme (AMD1) is a key enzyme involved in the synthesis of spermine (SPM) and spermidine (SPD), which are associated with multifarious cellular processes. It is also found to be an oncogene in multiple cancers and a potential target for tumor therapy. Nevertheless, the role AMD1 plays in hepatocellular carcinoma (HCC) is still unknown.

Methods: HCC samples were applied to detect AMD1 expression and evaluate its associations with clinicopathological features and prognosis. Subcutaneous and orthotopic tumor mouse models were constructed to analyze the proliferation and metastasis of HCC cells after AMD1 knockdown or overexpression. Drug sensitive and tumor sphere assay were performed to investigate the effect of AMD1 on HCC cells stemness. Real-time quantitative PCR (qRT-PCR), western blot, immunohistochemical (IHC) and m6A-RNA immunoprecipitation (Me-RIP) sequencing/qPCR were applied to explore the potential mechanisms of AMD1 in HCC. Furthermore, immunofluorescence, co-IP (Co-IP) assays, and mass spectrometric (MS) analyses were performed to verify the proteins interacting with AMD1.

Results: AMD1 was enriched in human HCC tissues and suggested a poor prognosis. High AMD1 level could promote SRY-box transcription factor 2 (SOX2), Kruppel like factor 4 (KLF4), and NANOG expression of HCC cells through obesity-associated protein (FTO)-mediated mRNA demethylation. Mechanistically, high AMD1 expression increased the levels of SPD in HCC cells, which could modify the scaffold protein, Ras GTPase-activating-like protein 1 (IQGAP1) and enhance the interaction between IQGAP1 and FTO. This interaction could enhance the phosphorylation and decrease the ubiquitination of FTO.

Conclusions: AMD1 could stabilize the interaction of IQGAP1 with FTO, which then promotes FTO expression and increases HCC stemness. AMD1 shows prospects as a prognostic predictor and a therapeutic target for HCC.
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http://dx.doi.org/10.1002/ctm2.352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7989706PMC
March 2021

Carrier-Free Immobilization of α-Galactosidase as Nano-Biocatalysts for Synthesizing Prebiotic α-Galacto-Oligosaccharides.

Molecules 2021 Feb 25;26(5). Epub 2021 Feb 25.

School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

α-Galacto-oligosaccharides (α-GOSs) have great functions as prebiotics and therapeutics. This work established the method of batch synthesis of α-GOSs by immobilized α-galactosidase for the first time, laying a foundation for industrial applications in the future. The α-galactosidase from L63 was immobilized as cross-linked enzyme aggregates (CLEAs) nano-biocatalyst through enzyme precipitating and cross-linking steps without using carriers. Among the tested agents, the ammonium sulfate showed high precipitation efficacy and induced regular structures of α-galactosidase CLEAs (Aga-CLEAs) that had been analyzed by scanning electron microscopy and Fourier-transform infrared spectroscopy. Through optimization by response surface methodology, the ammonium sulfate-induced Aga-CLEAs achieved a high activity recovery of around 90% at 0.55 U/mL of enzymes and 36.43 mM glutaraldehyde with cross-linking for 1.71 h. Aga-CLEAs showed increased thermal stability and organic solvent tolerance. The storage ability was also improved since it maintained 74.5% activity after storing at 4 °C for three months, significantly higher than that of the free enzyme (21.6%). Moreover, Aga-CLEAs exhibited excellent reusability in the α-GOSs synthesis from galactose, retaining above 66% of enzyme activity after 10 batch reactions, with product yields all above 30%.
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http://dx.doi.org/10.3390/molecules26051248DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956481PMC
February 2021

Healthcare Worker's Mental Health and Their Associated Predictors During the Epidemic Peak of COVID-19.

Psychol Res Behav Manag 2021 24;14:221-231. Epub 2021 Feb 24.

School of Public Health, Xuzhou Medical University, Xuzhou, Jiangsu, 221004, People's Republic of China.

Introduction: The outbreak of the coronavirus disease 2019 (COVID-19) poses an unprecedented challenge to healthcare workers (HCWs) globally. This study investigated potential factors related to depression, anxiety, and stress in a sample of Chinese HCWs during the peak of the COVID-19 epidemic.

Methods: An online survey was distributed to Chinese HCWs using respondent-driven sampling. Data were collected between February 13th and February 20th, 2020, immediately following the COVID-19 contagion peak in Hubei. A total of 1208 respondents were eligible for analysis. Mental health problems and social support were measured by the Depression Anxiety Stress Scales-21 (DASS-21) and the Perceived Social Support Scale (PSS).

Results: The prevalence rates of depression, (DASS-depression > 9) anxiety (DASS-anxiety > 7) and stress (DASS-stress > 14) were 37.8%, 43.0% and 38.5%, respectively. Multivariate logistic regressions revealed that stress, anxiety, and depression were positively related to lower levels of social support, longer working hours, discrimination experience and workplace violence. The scarcity of medical equipment was correlated with increased stress and depression. Chinese HCWs working at COVID 19 designated hospitals were more likely to report anxiety. Additionally, volunteering to work in the frontline health facilities was inversely associated with depression.

Conclusion: Mental health problems among Chinese HCWs were alarming during the peak of the COVID-19 epidemic. Health facilities require appropriate and standing services that address the mental health of healthcare workers, particularly during epidemic outbreaks.
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http://dx.doi.org/10.2147/PRBM.S290931DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918562PMC
February 2021

Insight into the amorphous nickel-iron (oxy)hydroxide catalyst for efficient oxygen evolution reaction.

J Colloid Interface Sci 2021 Jun 10;591:307-313. Epub 2021 Feb 10.

State Key Laboratory for Powder Metallurgy, Central South University, Changsha 410083, China. Electronic address:

The specific roles of Ni and Fe in nickel-iron (oxy)hydroxide catalyst (NiFeO(OH)) are extensively discussed during oxygen evolution reaction (OER). However, there still remains controversy about whether Ni or Fe species as the dominate active site. In this work, we reported the NiFeO(OH) catalysts with varied atomic ratio of nickel and iron for OER to explore the dominate active site during OER processes. From the electrochemical performances, the similar Tafel slopes of catalysts with Fe species can achieve at a level of 40 mV dec, outperforming the Tafel slopes of catalysts without Fe species. Thus, it can be concluded that the present Fe site can serve as the dominant active site in NiFeO(OH) for OER. Meanwhile, the Ni species is proved as the OH adsorption site, which is beneficial to the Fe site to deliver a better OER performance. As a result, the catalyst with an optimal Ni/Fe interface (atomic ratio of 1 : 1.18) displays outstanding OER performances. It only requires a low overpotential of 250 mV to deliver current density of 10 mA cm and exhibits a small Tafel slope of 39 mV dec. This catalyst also shows remarkable stability with negligible potential decay after 50 h at a current density of 50 mA cm. This work offers a new sight into the specific roles of Ni and Fe in NiFeO(OH) for OER.
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http://dx.doi.org/10.1016/j.jcis.2021.02.020DOI Listing
June 2021

Signal transduction associated with lead-induced neurological disorders: A review.

Food Chem Toxicol 2021 Apr 14;150:112063. Epub 2021 Feb 14.

Department of Toxicology, School of Public Health, Guangxi Medical University, No. 22, Shuang-yong Rd, Nanning, 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning, China. Electronic address:

Lead is a heavy metal pollutant that is widely present in the environment. It affects every organ system, yet the nervous system appears to be the most sensitive and primary target. Although many countries have made significant strides in controlling Pb pollution, Pb poisoning continuous to be a major public health concern. Exposure to Pb causes neurotoxicity that ranges from neurodevelopmental disorders to severe neurodegenerative lesions, leading to impairments in learning, memory, and cognitive function. Studies on the mechanisms of Pb-induced nervous system injury have convincingly shown that this metal can affect a plethora of cellular pathways affecting on cell survival, altering calcium dyshomeostasis, and inducing apoptosis, inflammation, energy metabolism disorders, oxidative stress, autophagy and glial stress. This review summarizes recent knowledge on multiple signaling pathways associated with Pb-induced neurological disorders in vivo and in vitro.
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http://dx.doi.org/10.1016/j.fct.2021.112063DOI Listing
April 2021

Pharmaceutical immunoglobulin G impairs anti-carcinoma activity of oxaliplatin in colon cancer cells.

Br J Cancer 2021 04 9;124(8):1411-1420. Epub 2021 Feb 9.

Department of General Surgery, Molecular Oncology and Immunotherapy, Rostock University Medical Center, Schillingallee 69, 18057, Rostock, Germany.

Background: Recent evidence proves that intravenous human immunoglobulin G (IgG) can impair cancer cell viability. However, no study evaluated whether IgG application benefits cancer patients receiving chemotherapeutics.

Methods: Influence of pharmaceutical-grade human IgG on the viability of a series of patient-derived colon cancer cell lines with and without chemotherapeutic intervention was determined. Cell death was analysed flow cytometrically. In addition, the influence of oxaliplatin and IgG on the ERK1/2-signalling pathway was evaluated by western blots.

Results: We evaluated the effects of pharmaceutical IgG, such as PRIVIGEN IgG and Tonglu IgG, in combination with chemotherapeutics. We did not observe any significant effects of IgG on tumour cell viability directly; however, human IgG significantly impaired the anti-tumoral effects of oxaliplatin. Primary cancer cell lines express IgG receptors and accumulate human IgG intracellularly. Moreover, while oxaliplatin induced the activation of ERK1/2, the pharmaceutical IgG inhibited ERK1/2 activity.

Conclusions: The present study demonstrates that pharmaceutical IgG, such as PRIVIGEN IgG and Tonglu IgG, can impair the anti-carcinoma activity of oxaliplatin. These data strongly suggest that therapeutic IgG as co-medication might have harmful side effects in cancer patients. The clinical significance of these preclinical observations absolutely advises further preclinical, as well as epidemiological and clinical research.
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http://dx.doi.org/10.1038/s41416-021-01272-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8039037PMC
April 2021

The effect of miR-471-3p on macrophage polarization in the development of diabetic cardiomyopathy.

Life Sci 2021 Mar 5;268:118989. Epub 2021 Jan 5.

Institute of Pharmaceutical Innovation, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, College of Medicine, Wuhan University of Science and Technology, Wuhan, Hubei Province, China. Electronic address:

Aims: The imbalance of M1/M2 macrophage ratio promotes the occurrence of diabetic cardiomyopathy (DCM), but the precise mechanisms are not fully understood. The aim of this study was to investigate whether miR-471-3p/silent information regulator 1 (SIRT1) pathway is involved in the macrophage polarization during the development of DCM.

Methods: Immunohistochemical staining was used to detect M1 and M2 macrophages infiltration in the heart tissue. Flow cytometry was used to detect the proportion of M1 and M2 macrophages. Expression of miR-471-3p was quantified by real time quantitative-PCR. Transfection of miRNA inhibitor into RAW264.7 cells was performed to investigate the underlying mechanisms. Bioinformatics methods and western blotting were used to explore the target gene of miR-471-3p and further confirmed by dual luciferase reporter assay.

Key Findings: We observed that M1 macrophages infiltration in the heart of tissue in DCM while M2 type was decreased. M1/M2 ratio was increased significantly in bone marrow-derived macrophages (BMDMs) from db/db mice and in RAW264.7 cells treated with advanced glycation end products (AGEs). Meanwhile, miR-471-3p was significantly upregulated in RAW264.7 cells induced by AGEs and inhibition of miR-471-3p could reduce the inflammatory polarization of macrophages. Bioinformatics analysis identified SIRT1 as a target of miR-471-3p. Both dual luciferase reporter assay and western blotting verified that miR-471-3p negatively regulated SIRT1 expression. SIRT1 agonist resveratrol could downregulate the increased proportion of M1 macrophages induced by AGEs.

Conclusion: Our results indicated that the development of DCM was related to AGEs-induced macrophage polarized to M1 type through a mechanism involving the miR-471-3p/SIRT1 pathway.
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http://dx.doi.org/10.1016/j.lfs.2020.118989DOI Listing
March 2021

Extracellular vesicle-encapsulated microRNA-424 exerts inhibitory function in ovarian cancer by targeting MYB.

J Transl Med 2021 01 6;19(1). Epub 2021 Jan 6.

Department of Obstetrics and Gynecology, Linyi People's Hospital, No. 27, Eastern Section of Jiefang Road, Lanshan District, Linyi, Shandong, 276000, People's Republic of China.

Background: Recent studies have suggested a crucial role of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) in ovarian cancer treatment. We, therefore, set out to explore the mechanism through which MSC-derived EVs delivered microRNA-424 (miR-424) to influence the development of ovarian cancer.

Methods: Bioinformatics analyses were first performed to screen ovarian cancer-related differentially expressed genes and to predict regulatory miRNAs. Then, dual-luciferase reporter gene assay was carried out to verify the relationship between miR-424 and MYB. Subsequently, the characterized MSCs and isolated EVs were co-cultured with ovarian cancer cells, followed by determination of the expression patterns of miR-424, MYB, vascular endothelial growth factor (VEGF), and VEGF receptor (VEGFR), respectively. In addition, the effects of EVs-delivered miR-424 on the proliferation, migration, invasion and tube formation of ovarian cancer cells were assessed using gain- and loss-of-function approaches. Lastly, tumor xenograft was induced in nude mice to illustrate the influence of EVs-loaded miR-424 on ovarian cancer in vivo.

Results: Our data exhibited that MYB was highly-expressed and miR-424 was poorly-expressed in ovarian cancer. More importantly, MYB was identified as a target gene of miR-424. Additionally, the transfer of miR-424 by MSC-derived EVs was found to repress the proliferation, migration, and invasion of ovarian cancer cells, with a reduction in the expressions of VEGF and VEGFR. Furthermore, MSC-derived EVs over-expressing miR-424 could inhibit the proliferation, migration, and tube formation of human umbilical vein endothelial cells, and also suppressed tumorigenesis and angiogenesis of ovarian tumors in vivo.

Conclusion: Collectively, our findings indicate that MSC-derived EVs transfer miR-424 to down-regulate MYB, which ultimately led to the inhibition of the tumorigenesis and angiogenesis of ovarian cancer. Hence, this study offers a potential prognostic marker and a therapeutic target for ovarian cancer.
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http://dx.doi.org/10.1186/s12967-020-02652-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786507PMC
January 2021

Epidemiologic trends and prognostic risk factors of patients with pancreatic neuroendocrine neoplasms in the US: an updated population-based study.

Future Oncol 2021 Feb 6;17(5):549-563. Epub 2021 Jan 6.

Department of General Surgery, Molecular Oncology & Immunotherapy, Rostock University Medical Center, Schillingallee 69, 18057, Rostock, Germany.

We aimed to evaluate the incidence, mortality and survival outcome for patients with pancreatic neuroendocrine neoplasms (pNEN). Patients with pNEN were collected from the Surveillance, Epidemiology, and End Results (SEER) database. Incidence, mortality and average annual percentage change (AAPC) were calculated using SEER stat 8.3.6 and Joinpoint software. Survival outcome was estimated using Kaplan-Meier and Cox proportional hazard model. During 2000-2016, the incidence of pNEN significantly rose from 0.2647 to 1.0618 per 100,000 persons with an AAPC of 9.4; AAPC of mortality was 6.7. Prognostic improvement was revealed in 2010-2016, but not for late-stage pNEN, which had the highest risk of death. Efforts to improve prognosis of pNEN patients must focus on not only early detection, but also on improving therapy for late-stage disease.
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http://dx.doi.org/10.2217/fon-2020-0543DOI Listing
February 2021

Analyzing non-cancer causes of death of colorectal carcinoma patients in the US population for the years 2000-2016.

Cancer Med 2021 04 13;10(8):2740-2751. Epub 2020 Dec 13.

Department of General Surgery, Molecular Oncology and Immunotherapy, Rostock University Medical Center, Rostock, Germany.

Background: Colorectal cancer (CRC) treatment and patient survival improved greatly. Consequently an increased incidence of non-cancer-related deaths is observed. This study analyzed the causes of non-cancer death for people suffering from CRC based on the year of diagnosis, follow-up time, and patient's age.

Methods: The data from patients diagnosed with CRC in the years 2000-2016 were taken from the Surveillance, Epidemiology, and End Results 18 database. Patients were categorized according to: death from CRC, non-CRC cancer, and non-cancer. Constituent ratios and standardized mortality ratios (SMRs) were calculated to describe the death causes distribution and relative death risks.

Results: Between 2000 and 2016, a stable and rapid drop for the original diagnosis as death cause for CRC patients was observed (70.19% to 49.35%). This was coupled to an increase in non-cancer-associated death reasons (23.38% to 40.00%). The most common non-cancer death cause was heart disease, especially for elderly patients. However, deaths from accidents and adverse effects were frequent in younger CRC patients. Patients died from septicemia more often within the first follow-up year; however, a 6-fold increase in death from Alzheimer's disease was found for after at least 180 months follow-up time. The SMRs of all 25 non-cancer death causes initially decreased in all CRC subgroups, followed by an increase with follow-up times. Gradually decreasing SMR values were observed with increasing age of CRC patients.

Conclusions: These findings could help modify and sharpen preventive measures and clinical management and raise physician's awareness to potential non-CRC death risk factors for CRC patients.
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http://dx.doi.org/10.1002/cam4.3673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026921PMC
April 2021

Mental Health Problems in Chinese Healthcare Workers Exposed to Workplace Violence During the COVID-19 Outbreak: A Cross-Sectional Study Using Propensity Score Matching Analysis.

Risk Manag Healthc Policy 2020 3;13:2827-2833. Epub 2020 Dec 3.

School of Health Sciences, Wuhan University, Wuhan, People's Republic of China.

Objective: This study aimed at examining the effect of medical workplace violence (MWV) on the mental health of Chinese healthcare workers during the outbreak of coronavirus disease 2019 (COVID-19).

Methods: An anonymous online survey was issued to Chinese healthcare workers (N=1063) from 31 provinces and autonomous regions between February 13th and February 20th. Mental health was measured by the Chinese Depression Anxiety Stress Scales-21 (DASS-21). Medical workplace violence was measured using a single item, whether any type of workplace violence was experienced during the COVID-19 outbreak. Propensity score matching was used to assess the impact of MWV on mental health.

Results: Out of 1063, 217 (20.4%) reported experiencing MWV during the COVID-19 outbreak. Before matching, MWV was correlated with elevated mental health problems (b=8.248, p<0.001), after adjusting for other variables. After matching, Chinese healthcare workers who experienced MWV were more likely to suffer from mental health problems than those who did not.

Conclusion: MWV exerts a detrimental effect on mental health among Chinese healthcare workers during the COVID-19 outbreak. It is necessary to create a more supportive and safer work environment for healthcare workers at this special context of the COVID-19 outbreak.
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http://dx.doi.org/10.2147/RMHP.S279170DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7721299PMC
December 2020

Sodium P-aminosalicylic Acid Inhibits Manganese-Induced Neuroinflammation in BV2 Microglial Cells via NLRP3-CASP1 Inflammasome Pathway.

Biol Trace Elem Res 2021 Sep 6;199(9):3423-3432. Epub 2020 Nov 6.

Department of Toxicology, School of Public Health, Guangxi Medical University, No. 22, Shuang-yong Rd, Nanning, 530021, Guangxi, China.

Background: Sodium p-aminosalicylic acid (PAS-Na) was reported to exhibit anti-inflammatory effect in the nervous system. However, the mechanism by which PAS-Na exhibits anti-inflammatory effects on manganese (Mn)-stimulated BV2 microglia cells remains unclear. Thus, this study investigated the role of PAS-Na in Mn-stimulated BV2 microglial cells.

Methods: Microglia-like BV2 were treated with MnCl with or without the non-steroidal anti-inflammatory drug PAS-Na for 12 or 24 h to examine cell viability using MTT; for 24 or 48 h to examine levels of NLRP3, CASP1, IL-1β, and IL-18 mRNA using Real-Time quantitative PCR; for 48 h to examine levels of NLRP3 and CASP1 inflammasomes, measured by western blot analysis; and for 48 h to examine levels of inflammatory cytokines, measured by enzyme-linked immunosorbent assay.

Results: The MTT assay showed that PAS-Na produced significant neuroprotective effect by preventing Mn-induced inflammation in BV2 microglial cells. PAS-Na significantly concentration and time dependently inhibited Mn-induced production of NLRP3, CASP1, IL-1β, and IL-18.

Conclusion: Taken together, our results suggest that PAS-Na exerts anti-inflammatory effects in Mn-stimulated BV2 microglial cells via downregulation of NLRP3, CASP1, IL-1β, and I L-18. Furthermore, a high concentration and prolonged PAS-Na treatment appear necessary for its therapeutic efficacy. Taken together, we conclude that PAS-Na affords therapeutic efficacy in mitigating neurological conditions associated with neuroinflammation.
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http://dx.doi.org/10.1007/s12011-020-02471-7DOI Listing
September 2021

Interface engineering in CeO (111) facets decorated with CdSe quantum dots for photocatalytic hydrogen evolution.

J Colloid Interface Sci 2020 Nov 27;579:707-713. Epub 2020 Jun 27.

State Key Laboratory for Powder Metallurgy, Central South University, Changsha Lushan South Road 932, 410083, China. Electronic address:

The interfaces of heterostructures have been widely studied in the field of photocatalytic H evolution reaction (HER). In the present study, the CdSe QDs/CeO(111) heterostructures were synthesized by wet chemistry method. The CdSe QDs/CeO(111)-0.075 showed higher photocatalytic H evolution with 283.32 µmol gh, because of the enhanced light absorbance intensity and edge, lower recombination, higher separation and transfer, as well as longer lifetime of the photogenerated carrier. Density functional theory (DFT) calculations further confirmed that the enhanced HER activity of CdSe QDs/CeO(111) heterostructures is resulted from a stronger water adsorption, a lower energy barrier of water dissociation and a more optimal free energy of hydrogen adsorption than CdSe and CeO. The strategy of construction heterostructures provides a promising pathway for enhancing the performance of photocatalytic H evolution as well as other catalytic reactions.
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http://dx.doi.org/10.1016/j.jcis.2020.06.100DOI Listing
November 2020
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