Publications by authors named "Lili Liu"

645 Publications

Maternal urinary cadmium concentrations in early pregnancy in relation to prenatal and postpartum size of offspring.

J Trace Elem Med Biol 2021 Jul 16;68:126823. Epub 2021 Jul 16.

National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China. Electronic address:

Background: The impacts of environmental cadmium (Cd) exposure on birth size parameters including weight, length and head circumference (HC) have been reported in multiple studies. However, little remains known of the impacts of maternal Cd exposure during pregnancy on size during in utero development and during early childhood. The aim of this study was to comprehensively investigate impacts of maternal Cd exposure during pregnancy on the size of offspring in utero (from 24 weeks pregnancy) until six months of age.

Methods: Pregnant mothers were recruited as part of an ongoing prospective birth cohort study based in Guangdong, China. Maternal urine samples were collected in the first and third trimesters of pregnancy, in which Cd concentrations were measured by inductively couple plasma mass spectrometry (ICPMS). In utero size indicators at 24 and 32 week of gestation, including biparietal diameter (BPD), abdominal circumference (AC), femur length (FL) and HC were derived from ultrasound examinations. Anthropometric measures of weight, height and HC at birth and one, three and six months of age were also collected. Associations of size measures at the various time points with maternal urinary Cd concentrations were assessed using linear regression models.

Results: The median urinary Cd concentration was 1.00 and 0.98 μg/g creatinine in the first and third trimesters respectively. In univariate analysis, increased maternal Cd levels in the first trimester were associated with decreased HC (-0.17 cm/ug/g urinary Cd) at birth, and the association was particularly pronounced among males (-0.30 cm/ug/g urinary Cd). First trimester Cd exposure was also found to be significantly associated with decreased infant weight at three and six months of age among girls (-101 g/ug/g and -97 g/ug/g urinary Cd, respectively). Associations of similar magnitude were observed after adjustment for various maternal factors. No significant associations were observed with infant size measures or with measures of Cd in the third trimester.

Conclusions: Our detailed study suggests that the first trimester is particularly critical window of susceptibility to sex-specific effects of Cd on size parameters at birth, with some effects persisting to six months of age. These compelling sex-dependent effects on HC and body weight warrant future studies examining longer-term health effects of pregnancy-related Cd exposures.
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http://dx.doi.org/10.1016/j.jtemb.2021.126823DOI Listing
July 2021

Mobile alert app to engage community volunteers to help locate missing persons with dementia.

PLoS One 2021 19;16(7):e0254952. Epub 2021 Jul 19.

School of Public Health Sciences, Faculty of Health, University of Waterloo, Waterloo, Ontario, Canada.

The prevalence of persons living with dementia and at risk of going missing is rising. In this study, we engaged persons living with dementia, care partners, police services, search and rescue organizations, and health and social service providers to develop Community ASAP, a mobile alert system that engages community citizens, as volunteers, to look out for persons with dementia reported missing. We completed three phases of development and evaluation of the usability and functionality of the alert system with stakeholders in three Canadian provinces. In this paper we describe features of the Community ASAP and the findings of these evaluation phases.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0254952PLOS
July 2021

Overexpression of PELP1 in Lung Adenocarcinoma Promoted E Induced Proliferation, Migration and Invasion of the Tumor Cells and Predicted a Worse Outcome of the Patients.

Pathol Oncol Res 2021 2;27:582443. Epub 2021 Apr 2.

Department of Pathology, Harbin Medical University-Daqing, Daqing, China.

The expression of Proline-, glutamic acid-, and leucine-rich protein 1 (PELP1) has been reported to be dysregulated in non-small cell lung carcinoma, especially in lung adenocarcinoma (LUAD). Therefore, we aimed to investigate the functional and prognostic roles of PELP1 in LUAD in this study. We first immunolocalized PELP1 in 76 cases of LUAD and 17 non-pathological or tumorous lung (NTL) tissue specimens and correlated the findings with the clinicopathological parameters of the patients. We then performed analysis including MTT, flow cytometry, wound healing, and transwell assays in order to further explore the biological roles of PELP1 in 17-β-estradiol (E) induced cell proliferation, migration, and invasion of LUAD cells. We subsequently evaluated the prognostic significance of PELP1 in LUAD patients using the online survival analysis tool Kaplan-Meier Plotter. The status of PELP1 immunoreactivity in LUAD was significantly higher than that in the NTL tissues and significantly positively correlated with less differentiated features of carcinoma cells, positive lymph node metastasis, higher clinical stage as well as the status of ERα, ERβ, and PCNA. study did reveal that E promoted cell proliferation and migration and elevated PELP1 protein level in PELP1-high A549 and H1975 cells but not in PELP1-low H-1299 cells. Knock down of PELP1 significantly attenuated E induced cell proliferation, colony formation, cell cycle progress as well as migration and invasion of A549 and H1975 cells. Kaplan-Meier Plotter revealed that LUAD cases harboring higher PELP1 expression had significantly shorter overall survival. In summary, PELP1 played a pivotal role in the estrogen-induced aggressive transformation of LUAD and could represent adverse clinical outcome of the LUAD patients.
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http://dx.doi.org/10.3389/pore.2021.582443DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8262236PMC
April 2021

Estimating the Prevalence of Asymptomatic COVID-19 Cases and Their Contribution in Transmission - Using Henan Province, China, as an Example.

Front Med (Lausanne) 2021 23;8:591372. Epub 2021 Jun 23.

Department of Biostatistics, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, China.

Novel coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-COV-2), is now sweeping across the world. A substantial proportion of infections only lead to mild symptoms or are asymptomatic, but the proportion and infectivity of asymptomatic infections remains unknown. In this paper, we proposed a model to estimate the proportion and infectivity of asymptomatic cases, using COVID-19 in Henan Province, China, as an example. We extended the conventional susceptible-exposed-infectious-recovered model by including asymptomatic, unconfirmed symptomatic, and quarantined cases. Based on this model, we used daily reported COVID-19 cases from January 21 to February 26, 2020, in Henan Province to estimate the proportion and infectivity of asymptomatic cases, as well as the change of effective reproductive number, . The proportion of asymptomatic cases among COVID-19 infected individuals was 42% and the infectivity was 10% that of symptomatic ones. The basic reproductive number = 2.73, and dropped below 1 on January 31 under a series of measures. The spread of the COVID-19 epidemic was rapid in the early stage, with a large number of asymptomatic infected individuals having relatively low infectivity. However, it was quickly brought under control with national measures.
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http://dx.doi.org/10.3389/fmed.2021.591372DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260942PMC
June 2021

The growth of plants and indigenous bacterial community were significantly affected by cadmium contamination in soil-plant system.

AMB Express 2021 Jul 10;11(1):103. Epub 2021 Jul 10.

School of Life Sciences, Hunan University of Science and Technology, Xiangtan, 411201, People's Republic of China.

Concentrations of heavy metals continue to increase in soil environments as a result of both anthropogenic activities and natural processes. Cadmium (Cd) is one of the most toxic heavy metals and poses health risks to both humans and the ecosystem. Herein, we explore the impacts of Cd on a soil-plant system composed of oilseed rapes (Brassica napus and Brassica juncea) and bacteria. The results showed that Cd accumulation within tissues of two species of oilseed rapes enhanced with increasing concentrations of Cd in soils, and Cd treatment decreased their chlorophyll content and suppressed rapeseeds growth. Meanwhile, Cd stress induced the changes of antioxidative enzymes activities of both B. napus and B. juncea. Response to Cd of bacterial community was similar in soil-two species of oilseed rapes system. The impact of Cd on the bacterial communities of soils was greater than bacterial communities of plants (phyllosphere and endophyte). The α-diversity of bacterial community in soils declined significantly under higher Cd concentration (30 mg/kg). In addition, soil bacterial communities composition and structure were altered in the presence of higher Cd concentration. Meanwhile, the bacterial communities of bulk soils were significantly correlated with Cd, while the variation of rhizosphere soils bacterial communities were markedly correlated with Cd and other environmental factors of both soils and plants. These results suggested that Cd could affect both the growth of plants and the indigenous bacterial community in soil-plant system, which might further change ecosystem functions in soils.
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http://dx.doi.org/10.1186/s13568-021-01264-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272791PMC
July 2021

Early Screening of Children With Autism Spectrum Disorder Based on Electroencephalogram Signal Feature Selection With L1-Norm Regularization.

Front Hum Neurosci 2021 22;15:656578. Epub 2021 Jun 22.

National Engineering Laboratory for Education Big Data, Faculty of Artificial Intelligence Education, Central China Normal University, Wuhan, China.

Early screening is vital and helpful for implementing intensive intervention and rehabilitation therapy for children with autism spectrum disorder (ASD). Research has shown that electroencephalogram (EEG) signals can reflect abnormal brain function of children with ASD, and screening with EEG signals has the characteristics of good real-time performance and high sensitivity. However, the existing EEG screening algorithms mostly focus on the data analysis in the resting state, and the extracted EEG features have some disadvantages such as weak representation capacity and information redundancy. In this study, we utilized the event-related potential (ERP) technique to acquire the EEG data of the subjects under positive and negative emotional stimulation and proposed an EEG Feature Selection Algorithm based on L1-norm regularization to perform screening of autism. The proposed EEG Feature Selection Algorithm includes the following steps: (1) extracting 20 EEG features from the raw data, (2) classification with support vector machine, (3) selecting appropriate EEG feature with L1-norm regularization according to the classification performance. The experimental results show that the accuracy for screening of children with ASD can reach 93.8% and 87.5% under positive and negative emotional stimulation and the proposed algorithm can effectively eliminate redundant features and improve screening accuracy.
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http://dx.doi.org/10.3389/fnhum.2021.656578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8259622PMC
June 2021

Development trajectory for the temporal and spatial evolution of the resilience of regional tourism environmental systems in 14 cities of Gansu Province, China.

Environ Sci Pollut Res Int 2021 Jul 6. Epub 2021 Jul 6.

Department of Economy and Management, Lanzhou University of Technology, Lanzhou, 730050, China.

The rapid development of the urban economy in China and the accompanying income growth experienced by urban residents have increased demand for tourism and leisure, which has brought pressure on the urban tourism environment system (UTES), making the contradiction between tourism economic development and the ecological environment increasingly acute. While seeking to rationalize the economic, social, and ecological benefits of tourism, reducing the fragility of the UTES and improving its anti-interference and recovery capabilities have become attracted significant attention from scholars in China and elsewhere. This paper establishes a definition of resilience for an UTES and constructs an evaluation index system for it in terms of the social, economic, and ecological environments. It also establishes an entropy weight-TOPSIS resilience evaluation model to measure resilience in regional systems, using ArcGIS to analyze the standard deviation ellipse and center of the gravity track of the resilience. System dynamics was used to construct diagrams of causal relationships and stock flow for the constituent elements of UTES to show the mechanisms that promote its resilience. This paper investigates 14 cities of Gansu Province in particular to simulate the resilience model of a regional system.
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http://dx.doi.org/10.1007/s11356-021-14932-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260156PMC
July 2021

Liquor Flavour Is Associated With the Physicochemical Property and Microbial Diversity of Fermented Grains in Waxy and Non-waxy Sorghum () During Fermentation.

Front Microbiol 2021 17;12:618458. Epub 2021 Jun 17.

College of Agronomy, State Key Laboratory of Crop Stress Biology in Arid Areas/Northwest A&F University, Yangling, China.

The fermentation process of Chinese Xifeng liquor involves numerous microbes. However, the sources of microbes in fermented grain and the link between liquor flavour and physicochemical properties and microbial diversity during fermentation still remain unknown. Herein, two waxy (JiNiang 2 [JN-2] and JinNuo 3 [JN-3]) and four non-waxy (JiZa 127 [JZ-127], JinZa 34 [JZ-34], LiaoZa 19 [LZ-19], and JiaXian [JX]) sorghum varieties were selected for the comprehensive analysis of the relationship between liquor flavour and the physicochemical properties and microbial diversity of fermented grains. Results showed that ethyl acetate was the main flavour component of JZ-127, JZ-34, and JX, whereas ethyl lactate was mainly detected in JN-2, JN-3, and LZ-19. Ethyl lactate accounted for half of the ethyl acetate content, and JX exhibited a higher liquor yield than the other sorghum varieties. The fermented grains of waxy sorghum presented higher temperature and reducing sugar contents but lower moisture and starch contents than their non-waxy counterparts during fermentation. We selected JN-3 and JX sorghum varieties to further investigate the microbial changes in the fermented grains. The bacterial diversity gradually reduced, whereas the fungal diversity showed nearly no change in either JN-3 or JX. was the most abundant bacterial genus, and its level rapidly increased during fermentation. The abundance of accounted for the total proportion of bacteria in JX, and it was higher than that in JN-3. was the most abundant fungal genus in JX, but its abundance accounted for a small proportion of fungi in JN-3. Four esters and five alcohols were significantly positively related to Proteobacteria, Bacteroidetes, and Actinobacteria; Alphaproteobacteria, Actinobacteria, and Bacteroidia; Bacillales, Bacteroidales, and Rhodospirillales; and , , and . This positive relation is in contrast with that observed for Firmicutes, Bacilli, Lactobacillales, and . Meanwhile, was the only fungal microorganism that showed a significantly negative relation with such compounds (except for butanol and isopentanol). These findings will help in understanding the fermentation mechanism and flavour formation of fermented Xifeng liquor.
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http://dx.doi.org/10.3389/fmicb.2021.618458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247930PMC
June 2021

The Effects of Cardiometabolic Factors on the Association Between Serum Uric Acid and Chronic Kidney Disease in Chinese Middle-Aged and Older Population: A Mediation Analysis.

Front Endocrinol (Lausanne) 2021 18;12:702138. Epub 2021 Jun 18.

Department of Endocrinology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Introduction: To explore whether dyslipidemia, hyperglycemia or hypertension has mediating effect on the association between serum uric acid (SUA) and the development of chronic kidney disease (CKD).

Methods: We conducted a mediation analysis to explore the potential mediating effects of systolic blood pressure (SBP), diastolic blood pressure (DBP), blood glucose, triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) on the association between SUA and estimated glomerular filtration rate (eGFR). The data were obtained from China Health and Retirement Longitudinal Study (CHARLS), covering 5,762 individuals.

Results: SUA had a negative dose-response total effect on eGFR ( -3.11, 95% CI -3.40 to -2.82, -value<0.001). The linear regression between SUA and seven potential mediators indicated that blood glucose ( 0.80, 95% CI 0.18 to 1.42, -value=0.012), TG ( 10.01, 95% CI 8.22 to 11.79, value<0.001), TC ( 2.64, 95% CI 1.83 to 3.45, -value<0.001), HDL-C ( -0.27, 95% CI -0.52 to -0.02, value=0.034) and LDL-C ( 1.15, 95% CI 0.49 to 1.80, -value=0.001) all had significant dose-response association with SUA, but SBP and DBP showed no significant association with SUA. In terms of the association between potential mediators and eGFR, only TG ( 0.003, 95% CI -0.001 to 0.01, -value=0.117) and HDL-C ( 0.01, 95% CI -0.02 to 0.04, -value=0.444) did not have significant linear association with eGFR. The linear regression showed that SUA was directly associated with eGFR (value<0.001).

Conclusions: This study supported that the association between SUA and the risk of CKD was not mediated by hypertension, hyperglycemia or dyslipidemia.
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http://dx.doi.org/10.3389/fendo.2021.702138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253158PMC
June 2021

Genome-wide DNA methylation profiling and gut flora analysis in intestinal polyps patients.

Eur J Gastroenterol Hepatol 2021 Aug;33(8):1071-1081

Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: The intestinal polyp is the precancerous lesion of colorectal cancer. DNA methylation and intestinal microbiota may play an important role in the development of intestinal polyp.

Materials And Methods: In this study, we included 10 patients with intestinal polyps who received the colonoscopy examination. We applied the Illumina Human Methylation 850K array to investigate the epigenome-wide DNA methylation patterns. Then, we filtered out the hub genes in the protein-protein interaction networks using functional epigenetic modules analysis. We also analyzed the colonizing bacteria on the surface of polyps compared with those in normal colonic mucosal epithelium with 16S ribosomal DNA sequencing.

Results: We identified 323 hypermethylated sites and 7992 hypomethylated sites between intestinal polyps and normal samples. Five hub genes, including CREB1, LPA, SVIL and KRT18, were identified in five modules. Hypomethylation of CREB1 is a candidate marker of colorectal adenoma. Gut microbiota analysis showed that Butyricicoccus was significantly decreased in the intestinal polyp groups.

Conclusion: In conclusion, we identified DNA methylation disparities in intestinal polyps compared with normal tissue, of which methylation of CREB1 may hold clinical significance in colorectal cancer progress. Colonizing bacteria in the colonic epithelium might be related to the formation of intestinal polyps.
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http://dx.doi.org/10.1097/MEG.0000000000002181DOI Listing
August 2021

[Analysis of three antipyretic analgesic drugs by open-tubular capillary electrochromatography].

Se Pu 2020 Sep;38(9):1107-1114

Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

The advantages of capillary electrophoresis, such as small sample consumption, high separation efficiency, and multiple separation modes, have been known for decades. However, exploring unique capillary electrophoresis techniques for the analysis of fluid drugs in living bio-systems remains an important and urgent task. Owing to the similar structures and mass-to-charge ratios of antipyretic analgesic drugs, efficient baseline separation of these analytes by capillary zone electrophoresis method cannot be easily achieved. Micellar electrokinetic chromatography can improve the baseline separation of these drugs, but the substantial amounts of non-volatile surfactants (such as sodium dodecyl sulfate, sodium dodecyl sulfonate, sodium deoxycholate and cetylammonium bromide) in running buffer solutions would pollute the ion source during mass spectrometric analysis. For this reason, it is difficult to analyze unknown drugs by capillary electrophoresis-electrospray ionization-mass spectrometry. To overcome these drawbacks, much attention has been paid to capillary electrochromatography (CEC) because combines the high separation efficiency of capillary electrophoresis with the high selectivity of high performance liquid chromatography (HPLC). Recent challenges encountered in open-tubular capillary electrochromatography (OT-CEC) expanding the range of suitable functional polymer monomers and improvement of the separation efficiency by tuning the characteristics of the polymer coatings without using any organic solvent additives. In this study, a protocol based on OT-CEC using a block co-polymer coating is proposed for the analysis of three test antipyretic analgesic drugs (4-aminoantipyrine, antipyrine and phenacetin), without adding organic solvents and surfactants in the running buffer solutions. First, an amphiphilic block co-poly(styrene-co-glycidyl methacrylate) (P(St-GMA)), was synthesized by reversible addition-fragmentation chain transfer polymerization under mild conditions. Then, P(St-GMA) was coated onto the capillary surface, and an OT-CEC analysis was performed. Next, the effect of some key factors, including the polymerization time for obtaining P(St-GMA) with different molecular weights, coating concentrations of the block copolymer, the species of the running buffer solutions, pH and concentrations of the running buffer solutions, and organic solvent additives, on the OT-CEC separation efficiency were investigated. Under the optimized conditions with 50.0 mmol/L NaAc-HAc as the running buffer solution at pH 5.7, the three test antipyretic analgesic drugs were base-line separated by the constructed OT-CEC system. Good linear relationships between peak area and concentration of the test analytes in the range of 8.0-2.5×10 μmol/L were obtained ( ≥ 0.995). The limits of detection (LODs) were 1.0-2.5 μmol/L. Furthermore, the reason for the OT-CEC separation efficiency was clarified based on the decreased electro-osmotic flow in the coated capillary compared with that in the uncoated capillary. Finally, the proposed OT-CEC assay without using any organic solvents and surfactants as additives was applied for analysis of the three test antipyretic analgesic drugs in rat serum samples. Importantly, it was found that despite peak tailing, the OT-CEC separation efficiency of the drugs was dramatically enhanced because the block co-polymer could self-assemble in the solution and form pseudo-micelles, which further increased the interactions between the P(St-GMA) and these drugs. Our results not only reveal the great potential of block co-polymer coatings in OT-CEC for the analysis of drugs in real biological samples, but also serve asa platform for the preparation of diverse block co-polymers to be used in OT-CEC analysis. We believe that in the near future, the peak tailing problem in OT-CEC analysis can be resolved by using the designed unique block co-polymers, which possess a greater number of functional sites, as coatings and by appropriately tuning the interactions between the analytes and the coatings.
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http://dx.doi.org/10.3724/SP.J.1123.2020.01006DOI Listing
September 2020

Link-Correlation-Aware Opportunistic Routing in Low-Duty-Cycle Wireless Networks.

Sensors (Basel) 2021 Jun 1;21(11). Epub 2021 Jun 1.

Shanghai Lilith Technology Corporation, Jiading District, Shanghai 200233, China.

In low-duty-cycle wireless networks with unreliable and correlated links, Opportunistic Routing (OR) is extremely costly because of the unaligned working schedules of nodes within a common candidate forwarder set. In this work, we propose a novel polynomial-time node scheduling scheme considering link correlation for OR in low-duty-cycle wireless networks (LDC-COR), which significantly improves the performance by assigning nodes with low correlation to a common group and scheduling the nodes within this group to wake up simultaneously for forwarding packets in a common cycle. By taking account of both link correlation and link quality, the performance of the expected transmission count (ETX) is improved by adopting the LDC-COR protocol. As a result, the energy consumption of low-duty-cycle OR is significantly reduced. LDC-COR only requires the information of one-hop neighboring nodes which introduces minimal communication overhead. The proposed LDC-COR bridges the gap between the nodes' limited energy resource and the application lifetime requirements. We evaluate the performance of LDC-COR with extensive simulations and a physical wireless testbed consisting of 20 TelosB nodes. The evaluation results show that both transmission efficiency and energy consumption of low-duty-cycle OR are significantly improved with only a slight increase of end-to-end delay.
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http://dx.doi.org/10.3390/s21113840DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199512PMC
June 2021

Daphnetin inhibits spinal glial activation via Nrf2/HO-1/NF-κB signaling pathway and attenuates CFA-induced inflammatory pain.

Int Immunopharmacol 2021 Jun 26;98:107882. Epub 2021 Jun 26.

Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China. Electronic address:

Daphnetin (7, 8-dihydroxycoumarin, DAPH), a coumarin derivative isolated from Daphne odora var., recently draws much more attention as a promising drug candidate to treat neuroinflammatory diseases due to its protective effects against neuroinflammation. However, itscontribution to chronic inflammatory pain is largely unknown. In the current work, we investigated the effects of DAPH in a murine model of inflammatory pain induced by complete Freund's adjuvant (CFA) and its possible underlying mechanisms. Our results showed that DAPH treatment significantly attenuated mechanical allodynia provoked by CFA. A profound inhibition of spinal glial activation, followed by attenuated expression levels of spinal pro-inflammatory cytokines, was observed in DAPH-treated inflammatory pain mice. Further study demonstrated that DAPH mediated negative regulation of spinal NF-κB pathway, as well as its preferential activation of Nrf2/HO-1 signaling pathway in inflammatory pain mice. This study, for the first time, indicated that DAPH might preventthe development of mechanical allodynia in mice with inflammatory pain. And more importantly, these data provide evidence for the potential application of DAPH in the treatment of chronic inflammatory pain.
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http://dx.doi.org/10.1016/j.intimp.2021.107882DOI Listing
June 2021

Glucose Homeostasis Is Dysregulated in Ducks Infected with Duck Hepatitis B Virus.

Intervirology 2021 Jun 24:1-9. Epub 2021 Jun 24.

Department of Biochemistry and Molecular Biology, Guilin Medical University, Guilin, China.

Introduction: The association between hepatitis B virus (HBV) infection and the development of diabetes remains controversial. This study examined the effect of HBV infection on glucose homeostasis using a duck HBV (DHBV) model.

Methods: Plasma DHBV DNA was detected by quantitative polymerase chain reaction (PCR). Tissue infection of DHBV was determined by detecting DHBV covalently closed circular DNA (cccDNA) with a method of rolling circle amplification combined with cross-gap PCR, and verified by fluorescence in situ hybridization assay. An intravenous injection glucose tolerance test (GTT) was used to analyze the effect of DHBV infection on glucose tolerance.

Results: Of the finally included 97 domestic ducks, 53 (54.6%) were congenitally infected by DHBV. The positive rate of DHBV cccDNA in the liver, kidney, pancreas, and skeletal muscle of the infected ducks was 100, 75.5, 67.9, and 47.2%, respectively. The DHBV-infected ducks had higher blood glucose levels at 15 and 30 min post-load glucose (p < 0.01 and p < 0.001, respectively) in the GTT, much more individuals with greater glucose area under curve (p < 0.01), and a 57% impaired glucose tolerance (IGT) rate, as compared with noninfected controls. In addition, the subgroups of the infected ducks with DHBV cccDNA positive in skeletal muscle maintained the higher blood glucose level up to 2 h post-load glucose during the GTT and had a 76% IGT rate.

Conclusion: These results suggest that DHBV intrahepatic and extrahepatic infection impairs glucose tolerance, and thus evidence the association of DHBV infection with the dysregulation of glucose metabolism.
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http://dx.doi.org/10.1159/000516766DOI Listing
June 2021

Emodin Attenuated the Kidney Damage of High-Fat-Diet Mice via the Upregulation of Glucagon-Like Peptide-1 Receptor.

Biomed Res Int 2021 31;2021:6662704. Epub 2021 May 31.

Department of Endocrinology, Affiliated Zhongda Hospital of Southeast University, Nanjing, China.

Objective: Secretion of glucagon-like peptide 1 (GLP-1) and its effect on target organs were impaired in individuals with obesity. However, its mechanism needs to be further studied. We aim to explore the roles of the receptor of GLP-1 (GLP-1R) involved in high-fat-diet- (HFD-) induced kidney damage improved by emodin.

Methods: Male C57bl/6 mice were fed with HFD diet and therapied by emodin. NRK-52E cells were cultured and treated with palmitic acid or low-density lipoprotein cholesterol (LDL-C). Emodin was used to remedy the NRK-52E cell damage. GW9662 was administrated to block the function of peroxisome proliferator-activated receptor (PPAR-). GLP-1 in the plasma was measured by ELISA. PPAR- and GLP-1R in the kidney and NRK-52E cells were detected by western blotting. The interaction between PPAR- protein and GLP-1R promoter regions was observed by chromatin immunoprecipitation (ChIP).

Results: Postprandial GLP-1 levels in plasma, as well as PPAR- and GLP-1R, decreased in kidney tissue of HFD mice, while they were reserved by emodin treatment. Although PPAR- and GLP-1R were not downregulated by LDL-C, they were suppressed by palmitic acid. Interestingly, GLP-1R mRNA was detected by PCR in the mixture pulled down with PPAR- antibody. Additionally, downregulation of PPAR- and GLP-1R by palmitic acid was remanded by emodin. Moreover, GW9662, an inhibitor of PPAR-, abolished the protective effect of emodin.

Conclusion: The kidney damage of HFD mice seems to be alleviated by emodin via the upregulation of GLP-1R in kidney tissue.
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http://dx.doi.org/10.1155/2021/6662704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187071PMC
May 2021

Crystallization and Phase Transformations of Aluminum (Oxy)hydroxide Polymorphs in Caustic Aqueous Solution.

Inorg Chem 2021 Jul 21;60(13):9820-9832. Epub 2021 Jun 21.

Physical & Computational Science Directorate, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.

Gibbsite, bayerite, and boehmite are important aluminum (oxy)hydroxide minerals in nature and have been widely deployed in various industrial applications. They are also major components in caustic nuclear wastes stored at various U.S. locations. Knowledge of their crystallization and phase transformation processes contributes to understanding their occurrence and could help optimize waste treatment processes. While it has been reported that partial conversion of bayerite and gibbsite to boehmite occurs in basic solutions at elevated temperatures, systematic studies of factors affecting the phase transformation as well as the underlying reaction mechanisms are nonexistent, particularly in highly alkaline solutions. We explored the effects of sodium hydroxide concentrations (0.1-3 M), reaction temperatures (60-100 °C), and aluminum concentrations (0.1-1 M) on the crystallization and transformation of these aluminum (oxy)hydroxides. Detailed structural and morphological characterization by X-ray diffraction (XRD), scanning electron microscopy (SEM), and nuclear magnetic resonance (NMR) spectrometry revealed that these processes depend largely on the reaction temperature and the Al/OH ratio. When 1 ≤ Al/OH ≤ 2.5, the reactions favor formation of high-crystallinity precipitates, whereas at an Al/OH ratio of ≥2.5 precipitation ceases unless the Al concentration is higher than 1 M. We identified pseudoboehmite, bayerite, and gibbsite as intermediate phases to bayerite, gibbsite and boehmite, respectively, all of which transform via dissolution-reprecipitation. Gibbsite transforms to boehmite in both acidic and weak caustic environments at temperatures above 80 °C. However, a "bar-shaped" gibbsite morphology dominates in highly caustic environments (3 M NaOH). The findings enable a robust basis for the selection of various solid phases by tuning the reaction conditions.
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http://dx.doi.org/10.1021/acs.inorgchem.1c01111DOI Listing
July 2021

Confined Magnetic-Dielectric Balance Boosted Electromagnetic Wave Absorption.

Small 2021 Jun 18:e2100970. Epub 2021 Jun 18.

Laboratory of Advanced Materials, Shanghai Key Lab of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438, P. R. China.

Magnetic-dielectric property plays a critical significance for the functional expression toward advanced materials. Within nanoscale, the simultaneous regulation of the electrical and magnetic properties of electromagnetic (EM) wave absorption materials faces huge challenges. Herein, using the metal-organic frameworks (MOF) as templates, highly-dispersed ZnO and Co nanoparticles are uniformly confined inside graphited N-doped carbon skeleton, constructing the balanced EM property in the [email protected] absorbers. Meanwhile, a dynamics and symmetrical morphology optimization of MOF-derived [email protected] are dependent on the Co/Zn mass ratio and adjusting MOF frameworks, which evolves from the cube, truncated cube, dodecahedron, and to the final microsphere. Simultaneously, both the electronic conduction network and magnetic coupling network are compatible together in the in situ transformed [email protected] system. Boosted magnetic responding ability and unique magnetic coupling are verified by the off-axis electronic holography. Plentiful heterojunction interfaces and special electronic conduction paths can be built in this Co-Zn-MOF derivatives, facilitating the dielectric loss behaviors. As expected, MOF-derived [email protected] absorber displays outstanding EM wave absorption ability with strongest reflection loss value of -69.6 dB at only 1.9 mm thickness and wideband absorption covering 6.8 GHz at 2.4 mm. Confined EM balance provides new design strategy toward MOF-derived excellent MA materials and functional devices.
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http://dx.doi.org/10.1002/smll.202100970DOI Listing
June 2021

Arhgef2 regulates neural differentiation in the cerebral cortex through mRNA mA-methylation of Npdc1 and Cend1.

iScience 2021 Jun 24;24(6):102645. Epub 2021 May 24.

Laboratory of Medical Systems Biology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 510623 Guangzhou, China.

-methyladenosine (mA) is emerging as a vital factor regulating neural differentiation. Here, we report that deficiency of , a novel cause of a neurodevelopmental disorder we identified recently, impairs neurogenesis, neurite outgrowth, and synaptic formation by regulating mA methylation. knockout decreases expression of and total mA level significantly in the cerebral cortex. mA sequencing reveals that loss of reduces mA methylation of 1,622 mRNAs, including and which are both strongly associated with cell cycle exit and terminal neural differentiation. deficiency decreases mA methylations of the and mRNAs via down-regulation of Mettl14, and thereby inhibits the translation of and nuclear export of mRNAs. Overexpression of Mettl14, Npdc1, and Cend1 rescue the abnormal phenotypes in knockout mice, respectively. Our study provides a critical insight into a mechanism by which defective mediates mA-tagged target mRNAs to impair neural differentiation.
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http://dx.doi.org/10.1016/j.isci.2021.102645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185223PMC
June 2021

The changes in pyroptosis-related inflammatory factors in the peripheral blood of patients with Henoch-Schonlein purpura.

Ann Palliat Med 2021 Jun 7;10(6):6687-6693. Epub 2021 Jun 7.

Department of Laboratory, Donghai Branch of the Second Affiliated Hospital of Fujian Medical University, Quanzhou, China.

Background: Henoch-Schonlein purpura (HSP) is a common capillary allergic bleeding disease. To explore the variation of pyroptosis-related inflammatory factors level in the peripheral blood of patients with HSP.

Methods: A total of 87 HSP patients treated in our hospital from June 2020 to March 2021 were selected and divided into the renal impairment group (n=29) and the non-renal impairment group (n=58) according to the presence of hematuria and proteinuria. A total of 50 healthy individuals from the hospital were selected as the control group. The renal impairment and non-renal impairment groups were treated with a regular regimen of compound glycyrrhizin tablets and glucocorticoids, respectively. Serum interleukin (IL)-18, IL-1β, and peripheral caspase-1-positive cells were compared pre- and post-treatment among the three groups.

Results: The pre-treatment serum IL-1β levels in the renal impairment and non-renal impairment groups were significantly higher than that in the control group (P<0.01). After treatment, the IL-1β level in the non-renal impairment group was not significantly different from that in the control group (P>0.05). However, the IL-1β level in the renal impairment group post-treatment was significantly higher than that in the other two groups (P<0.01). The positive rate of caspase-1 expression in peripheral blood before treatment in the renal impairment group and non-renal impairment group was significantly higher than that in the control group (P<0.01). After treatment, the positive rate of caspase-1 expression in the non-renal impairment group was comparable to that in the control group (P>0.05), whereas the rate in the renal impairment group was significantly higher than that in the other two groups (P<0.01). After treatment, the serum IL-1β levels and caspase-1 positive rate in HSP patients who were responsive to treatment (as assessed by hematuria or proteinuria levels after treatment) were lower than that in patients who were unresponsive to treatment P<0.001), but not significantly different to the control group (P>0.05).

Conclusions: The levels of serum IL-1β and caspase-1 changed in response to alterations in the disease condition and treatment response in HSP patients, which suggested that pyroptosis-related inflammatory factors may have potential application value in predicting disease progression and efficacy of hormone therapy.
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http://dx.doi.org/10.21037/apm-21-1227DOI Listing
June 2021

Near-Infrared Spectroscopy Monitoring of Cerebral Oxygenation and Influencing Factors in Neonates from High-Altitude Areas.

Neonatology 2021 9;118(3):348-353. Epub 2021 Jun 9.

Pediatric Department, Peking University First Hospital, Beijing, China.

Background: Accurate detection of cerebral oxygen saturation (rSO2) may be useful for neonatal brain injury prevention, and the normal range of rSO2 of neonates at high altitude remained unclear.

Objective: To compare cerebral rSO2 and cerebral fractional tissue oxygen extraction (cFTOE) at high-altitude and low-altitude areas in healthy neonates and neonates with underlying diseases.

Methods: 515 neonates from low-altitude areas and 151 from Tibet were enrolled. These neonates were assigned into the normal group, hypoxic-ischemic encephalopathy (HIE) group, and other diseases group. Near-infrared spectroscopy was used to measure rSO2 in neonates within 24 h after admission. The differences of rSO2, pulse oxygen saturation (SpO2), and cFTOE levels were compared between neonates from low- and high-altitude areas.

Results: (1) The mean rSO2 and cFTOE levels in normal neonates from Tibet were 55.0 ± 6.4% and 32.6 ± 8.5%, significantly lower than those from low-altitude areas (p < 0.05). (2) At high altitude, neonates with HIE, pneumonia (p < 0.05), anemia, and congenital heart disease (p < 0.05) have higher cFTOE than healthy neonates. (3) Compared with HIE neonates from plain areas, neonates with HIE at higher altitude had lower cFTOE (p < 0.05), while neonates with heart disease in plateau areas had higher cFTOE than those in plain areas (p < 0.05).

Conclusions: The rSO2 and cFTOE levels in normal neonates from high-altitude areas are lower than neonates from the low-altitude areas. Lower cFTOE is possibly because of an increase in blood flow to the brain, and this may be adversely affected by disease states which may increase the risk of brain injury.
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http://dx.doi.org/10.1159/000514403DOI Listing
June 2021

Correction: Recovery of the self-cleaning property of silicon elastomers utilizing the concept of reversible coordination bonds.

Soft Matter 2021 Jun 9;17(24):6044. Epub 2021 Jun 9.

State Key Laboratory of Environmental-friendly Energy Materials, Southwest University of Science and Technology, Mianyang 621010, China.

Correction for 'Recovery of the self-cleaning property of silicon elastomers utilizing the concept of reversible coordination bonds' by Yuxing Shan et al., Soft Matter, 2020, 16, 8473-8481, DOI: .
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http://dx.doi.org/10.1039/d1sm90109eDOI Listing
June 2021

An Artificial Polyacrylonitrile Coating Layer Confining Zinc Dendrite Growth for Highly Reversible Aqueous Zinc-Based Batteries.

Adv Sci (Weinh) 2021 Jun 30;8(11):e2100309. Epub 2021 Mar 30.

Department of Chemistry, University of Venda, Thohoyandou, 0950, South Africa.

Aqueous rechargeable zinc-metal-based batteries are an attractive alternative to lithium-ion batteries for grid-scale energy-storage systems because of their high specific capacity, low cost, eco-friendliness, and nonflammability. However, uncontrollable zinc dendrite growth limits the cycle life by piercing the separator, resulting in low zinc utilization in both alkaline and mild/neutral electrolytes. Herein, a polyacrylonitrile coating layer on a zinc anode produced by a simple drop coating approach to address the dendrite issue is reported. The coating layer not only improves the hydrophilicity of the zinc anode but also regulates zinc-ion transport, consequently facilitating the uniform deposition of zinc ions to avoid dendrite formation. A symmetrical cell with the polymer-coating-layer-modified Zn anode displays dendrite-free plating/stripping with a long cycle lifespan (>1100 h), much better than that of the bare Zn anode. The modified zinc anode coupled with a Mn-doped V O cathode forms a stable rechargeable full battery. This method is a facile and feasible way to solve the zinc dendrite problem for rechargeable aqueous zinc-metal batteries, providing a solid basis for application of aqueous rechargeable Zn batteries.
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http://dx.doi.org/10.1002/advs.202100309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188195PMC
June 2021

CEP55 Positively Affects Tumorigenesis of Esophageal Squamous Cell Carcinoma and Is Correlated with Poor Prognosis.

J Oncol 2021 18;2021:8890715. Epub 2021 May 18.

Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou 510060, China.

Centrosomal protein 55 (CEP55) is a centrosome- and midbody-associated protein that is overexpressed in several cancers. However, the underlying molecular mechanism of CEP55-mediated progression and metastasis of esophageal squamous cell carcinoma (ESCC) is not clear. In the current study, we detected CEP55 mRNA by qRT-PCR while protein expression was detected by western blot analysis and immunohistochemistry (IHC). In addition, we knocked down CEP55 and investigated the ability of CEP55 to affect colony formation and migration. Here, we report that CEP55 mRNA and protein expression was significantly increased in ESCC. IHC staining showed that CEP55 expression correlated with TNM stage (=0.046) and lymph node metastases (=0.024). According to overall survival (OS) and disease-free survival (DFS), patients whose tumors expressed a higher level of CEP55 had a poorer prognosis than those with low expression level of CEP55. A multivariate analysis revealed that CEP55 expression was an independent prognostic indicator for patients with ESCC. Knockdown of CEP55 decreased the colony formation ability and migration of ESCC cells and also reduced the phosphorylation of Src, FAK, and ERK. Therefore, our study implied that CEP55 may be a valuable biomarker and a potential target in the treatment of patients with ESCC.
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http://dx.doi.org/10.1155/2021/8890715DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159646PMC
May 2021

Rutin Ameliorates Cadmium-Induced Necroptosis in the Chicken Liver via Inhibiting Oxidative Stress and MAPK/NF-κB Pathway.

Biol Trace Elem Res 2021 Jun 6. Epub 2021 Jun 6.

Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Department of Preventive Veterinary, College of Veterinary, Northeast Agricultural University, No. 600 Changjiang Street, Harbin, 150030, China.

Cadmium (Cd) is a recognized toxic metal and exerts serious hepatotoxicity in animals and humans. Rutin (RUT) is a dietary bioflavonoid with strong antioxidant and anti-inflammatory potential. However, little is known about the alleviating effect of RUT against Cd-induced liver necroptosis. The aim of this study was to ascertain the ameliorative mechanism of RUT on necroptosis triggered by Cd in chicken liver. One hundred twenty-eight 100-day-old Isa hens were randomly divided into four groups: the control group, RUT group, Cd + RUT cotreated group, and Cd group. Cd exposure prominently elevated Cd accumulation and the activities of liver function indicators (ALT and AST). Furthermore, the histopathological results, the overexpression of genes (RIPK1, RIPK3, and MLKL) related to the necroptosis pathway, and low Caspase 8 levels in Cd-exposed chicken liver indicated that Cd intoxication induced necroptosis in chicken liver. Meanwhile, Cd administration drastically increased the levels of oxidizing stress biomarkers (ROS production, MDA content, iNOS activity, and NO generation), and obviously reduced the activities of antioxidant enzymes (SOD, GPx, and CAT) and total antioxidant capacity (T-AOC) in chicken liver. Cd treatment promoted the expression of the main members of the MAPK and NF-κB pathways (JNK, ERK, P38, NF-κB, and TNF-α) and activated heat shock proteins (HSP27, HSP40, HSP60, HSP70, and HSP90). However, RUT application remarkably alleviated these Cd-induced variations and necroptosis injury. Overall, our study demonstrated that RUT might prevent Cd-induced necroptosis in the chicken liver by inhibiting oxidative stress and MAPK/NF-κB pathway.
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http://dx.doi.org/10.1007/s12011-021-02764-5DOI Listing
June 2021

Shark New Antigen Receptor (IgNAR): Structure, Characteristics and Potential Biomedical Applications.

Cells 2021 May 8;10(5). Epub 2021 May 8.

College of Life Science and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.

Shark is a cartilaginous fish that produces new antigen receptor (IgNAR) antibodies. This antibody is identified with a similar human heavy chain but dissimilar sequences. The variable domain (VNAR) of IgNAR is stable and small in size, these features are desirable for drug discovery. Previous study results revealed the effectiveness of VNAR as a single molecule or a combination molecule to treat diseases both in vivo and in vitro with promising clinical applications. We showed the first evidence of IgNAR alternative splicing from spotted bamboo shark (), broadening our understanding of the IgNARs characteristics. In this review, we summarize the discoveries on IgNAR with a focus on its advantages for therapeutic development based on its peculiar biochemistry and molecular structure. Proper applications of IgNAR will provide a novel avenue to understand its special presence in cartilaginous fishes as well as designing a number of drugs for undefeated diseases.
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http://dx.doi.org/10.3390/cells10051140DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151367PMC
May 2021

Self-consciousness and depression in precocious pubertal children.

J Int Med Res 2021 May;49(5):3000605211020227

Department of Pediatric Genetics, Metabolism and Endocrinology, West China Second University Hospital, School of Nursing, Sichuan University, Chengdu, Sichuan, China.

Objective: To explore self-consciousness and depression in children with precocious puberty (PP) and analyse its effect on children of both sexes.

Methods: Sixty children with PP and 60 non-PP children matched for sex and age participated in the study. Children were assessed using the Birleson Depression Self-Rating Scale for Children and the Piers-Harris Children's Self-Concept Scale.

Results: There were significant differences in physical appearance and attributes, anxiety, happiness and satisfaction between PP children and non-PP children. PP children had significantly higher depression than non-PP children. In the PP group, girls were significantly more prone to anxiety and unhappiness than boys. Conclusion: There were sex differences in the effect of PP on children's self-consciousness, and girls were more prone to anxiety and unhappiness. More attention should be paid to improving the physical and mental health of children with PP.
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http://dx.doi.org/10.1177/03000605211020227DOI Listing
May 2021

Prediction of the Blood-Brain Barrier (BBB) Permeability of Chemicals Based on Machine-Learning and Ensemble Methods.

Chem Res Toxicol 2021 Jun 28;34(6):1456-1467. Epub 2021 May 28.

Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Shenyang, Liaoning University, Shenyang 110036, China.

The ability of chemicals to enter the blood-brain barrier (BBB) is a key factor for central nervous system (CNS) drug development. Although many models for BBB permeability prediction have been developed, they have insufficient accuracy (ACC) and sensitivity (SEN). To improve performance, ensemble models were built to predict the BBB permeability of compounds. In this study, in silico ensemble-learning models were developed using 3 machine-learning algorithms and 9 molecular fingerprints from 1757 chemicals (integrated from 2 published data sets) to predict BBB permeability. The best prediction performance of the base classifier models was achieved by a prediction model based on an random forest (RF) and a MACCS molecular fingerprint with an ACC of 0.910, an area under the receiver-operating characteristic (ROC) curve (AUC) of 0.957, a SEN of 0.927, and a specificity of 0.867 in 5-fold cross-validation. The prediction performance of the ensemble models is better than that of most of the base classifiers. The final ensemble model has also demonstrated good accuracy for an external validation and can be used for the early screening of CNS drugs.
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http://dx.doi.org/10.1021/acs.chemrestox.0c00343DOI Listing
June 2021

Sex differences in the association between serum lipids and depressive symptoms: A longitudinal population-based study.

J Affect Disord 2021 08 14;291:154-162. Epub 2021 May 14.

Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, 324 Jing 5 road, Huaiyin District, Jinan 250021, China; Shandong Institute of Endocrine & Metabolic Diseases, Shandong First Medical University, Jinan, China 250021, China. Electronic address:

Background: Depression is an important global public health problem. Whether sex differences exist in the association between serum lipids and depressive symptoms is controversial. This study aimed to investigate sex differences in the association between dyslipidemia and depressive symptoms in the middle-aged and elderly population in China.

Methods: Data from the China Health and Retirement Longitudinal Study, which included 2 630 males and 2 929 females, were used. Serum lipids were measured using a standard enzymatic colorimetric technique. Depressive symptoms were evaluated by the 10-item Center for Epidemiologic Studies Depression Scale. Multivariable logistic regression was performed to evaluate the association between serum lipids and depressive symptoms in men and women separately. To preclude the effects of hypertension and diabetes, the above analysis was also performed among those free of hypertension and diabetes.

Results: Total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol were not associated with depressive symptoms. High-level triglycerides showed a marginally significant association with depressive symptoms in men (adjusted OR 1.35, 95% CI 0.91 to 1.99). In the population without hypertension and diabetes, high-levels TGs were only associated with the development of depressive symptoms in men (adjusted OR 2.04, 95% CI 1.20 to 3.47).

Limitations: Depression and chronic diseases were respectively identified by CESD-10 and self-report, which were subjective to some extent.

Conclusions: High-level triglycerides increased the risk of depressive symptoms in men, especially in the nonhypertensive and nondiabetic population. This study provided a basis for formulating sex-specific prevention strategies and treatment measures for depressive symptoms.
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http://dx.doi.org/10.1016/j.jad.2021.05.011DOI Listing
August 2021

Prediction Models for Prognosis of Cervical Cancer: Systematic Review and Critical Appraisal.

Front Public Health 2021 7;9:654454. Epub 2021 May 7.

Department of Epidemiology and Biostatistics, School of Public Health, Peking University Health Science Center, Beijing, China.

This work aims to systematically identify, describe, and appraise all prognostic models for cervical cancer and provide a reference for clinical practice and future research. We systematically searched PubMed, EMBASE, and Cochrane library databases up to December 2020 and included studies developing, validating, or updating a prognostic model for cervical cancer. Two reviewers extracted information based on the CHecklist for critical Appraisal and data extraction for systematic Reviews of prediction Modeling Studies checklist and assessed the risk of bias using the Prediction model Risk Of Bias ASsessment Tool. Fifty-six eligible articles were identified, describing the development of 77 prognostic models and 27 external validation efforts. The 77 prognostic models focused on three types of cervical cancer patients at different stages, i.e., patients with early-stage cervical cancer ( = 29; 38%), patients with locally advanced cervical cancer ( = 27; 35%), and all-stage cervical cancer patients ( = 21; 27%). Among the 77 models, the most frequently used predictors were lymph node status ( = 57; 74%), the International Federation of Gynecology and Obstetrics stage ( = 42; 55%), histological types ( = 38; 49%), and tumor size ( = 37; 48%). The number of models that applied internal validation, presented a full equation, and assessed model calibration was 52 (68%), 16 (21%), and 45 (58%), respectively. Twenty-four models were externally validated, among which three were validated twice. None of the models were assessed with an overall low risk of bias. The Prediction Model of Failure in Locally Advanced Cervical Cancer model was externally validated twice, with acceptable performance, and seemed to be the most reliable. Methodological details including internal validation, sample size, and handling of missing data need to be emphasized on, and external validation is needed to facilitate the application and generalization of models for cervical cancer.
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http://dx.doi.org/10.3389/fpubh.2021.654454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137851PMC
May 2021

LncRNA XIST promotes liver cancer progression by acting as a molecular sponge of miR-200b-3p to regulate ZEB1/2 expression.

J Int Med Res 2021 May;49(5):3000605211016211

Department of Medical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.

Objective: To evaluate the predictive value of long non-coding RNA (lncRNA) X-inactive specific transcript (XIST) for survival, and determine the involvement of miRNA(miR)-200b-3p and zinc finger E-box-binding homeobox (ZEB) 1/2 in the pro-tumor effect of lncRNA XIST in liver cancer.

Methods: We evaluated lncRNA XIST expression in liver cancer tissues and cell lines by quantitative reverse transcription polymerase chain reaction (RT-qPCR) and analyzed the correlation between its expression and overall survival of liver cancer patients by Kaplan-Meier analysis. Its effects on cell proliferation, migration, and invasion were analyzed by Cell-Counting Kit-8 and Transwell assays. The association between lncRNA XIST and miR-200b-3p, and the effects of lncRNA XIST on ZEB1/2 expression were explored using luciferase reporter assays, real-time PCR, and western blotting.

Results: The lncRNA XIST was significantly upregulated in liver cancer, and increased lncRNA XIST expression was associated with a poor prognosis. The lncRNA XIST promoted liver cancer cell proliferation, migration, and invasion , and acted as a molecular sponge for miR-200b-3p, and also regulated the expression of ZEB1/2 via miR-200b-3p.

Conclusion: The lncRNA XIST is an oncogenic lncRNA that promotes liver cancer metastasis, and its pro-metastatic phenotype can be partially attributed to the lncRNA XIST/miR-200b-3p/ZEB1/2 signaling axis.
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http://dx.doi.org/10.1177/03000605211016211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142537PMC
May 2021