Publications by authors named "Lijuan Wang"

905 Publications

Fasting Plasma Glucose Mediates the Prospective Effect of Maternal Metal Level on Birth Outcomes: A Retrospective and Longitudinal Population-Based Cohort Study.

Front Endocrinol (Lausanne) 2021 16;12:763693. Epub 2021 Nov 16.

Foshan Fetal Medicine Research Institute, Foshan Women and Children Hospital Affiliated to Southern Medical University, Foshan, China.

Objective: Previously, we found that the presence of maternal serum metals before the 24th week of gestation prospectively increased fasting plasma glucose (FPG) at 24-28 weeks. We further explored the prospective association between levels of metals and neonatal outcomes and assessed the mediating effects of FPG on these relationships.

Methods: A total of 7,644 pregnant women were included in a retrospective cohort study, and the relationships between metals [manganese (Mn), copper (Cu), lead (Pb), zinc (Zn), and magnesium (Mg)] and birth outcomes were explored. Quantile and linear regressions were performed to detect the shifts and associations between metals and neonatal size distribution focused on the 10th, 50th, and 90th percentiles. Mediation analysis was performed to assess the mediating effect of FPG on metals and birth outcomes.

Results: After adjustment, a 50% increase in Mn and Zn levels was related to a 0.136-cm (95% CI: 0.067-0.205) and 0.120-cm (95% CI: 0.046-0.193) increase in head circumference, respectively. Based on head circumference distribution, the magnitude of the association with Mn was smaller at the upper tail, while the magnitude of correlation with Zn was greater at the upper tail. A 50% increase in Mn and Zn levels was related to a 0.135-cm (95% CI: 0.058-0.212) and 0.095-cm (95% CI: 0.013-0.178) increase in chest circumference, respectively. The magnitude of the association with Mn increased with increasing chest circumference, while the magnitude of correlation with Zn decreased with increasing chest circumference. FPG explained 10.00% and 17.65% of the associations of Mn with head and chest circumference. A positive indirect effect of Zn associated with head circumference (0.004, 95% CI: 0.002-0.006) and chest circumference (0.005, 95% CI: 0.003-0.008) through FPG was also observed, and the estimated proportion of the mediating effect was 13.79% and 26.32%, respectively.

Conclusion: Maternal serum Mn and Zn levels before the 24th week of gestation may prospectively increase the circumference of the neonatal head and chest. FPG at 24-28 weeks had positive mediating effects on these relationships. Further research is needed to identify a balance between maternal blood glucose and birth size.
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http://dx.doi.org/10.3389/fendo.2021.763693DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8635137PMC
November 2021

Effect of Edaravone on MicroRNA Expression in Exosomes after Hepatic Ischemia-reperfusion Injury.

Curr Mol Pharmacol 2021 Nov 30. Epub 2021 Nov 30.

Department of Anesthesiology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, Hunan. China.

Background And Objective: Hepatic ischemia-reperfusion injury (HIRI) results in serious complications after liver resection and transplantation. Edaravone (ED) has a protective effect on IRI. This study was designed to evaluate whether ED could protect the liver of rats from HIRI injury and explored its exosomal miRNA-related mechanism.

Methods: The sham group, hepatic ischemia/reperfusion (IR group), and hepatic ischemia/reperfusion + edaravone (ED group) models were established. We determined the protective effect of ED by measuring alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), superoxide dismutase (SOD); enzyme-linked immunosorbent assay for tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β); hematoxylin-eosin staining and immunohistochemistry for histopathological changes. Exosomal miRNAs were subjected to second-generation sequencing to identify their differential expression. The results were analyzed using bioinformatics methods and validated using real-time quantitative polymerase chain reaction (RT-qPCR).

Results: HIRI rats showed higher levels of ALT, AST, oxidative stress, and inflammatory markers; ED attenuated these effects. The sequencing results showed 6 upregulated and 13 downregulated miRNAs in the IR vs. sham groups, 10 upregulated and 10 downregulated miRNAs in the ED vs. IR groups. PC-3p-190-42101 was screened as an overlapping differentially expressed miRNA, and RT-qPCR validation showed that its expression in HIRI rats was significantly decreased; ED prevented this downregulation. Moreover, the expression of PC-3P-190-42101 was significantly correlated with the level of inflammatory factors.

Conclusion: These findings indicate that ED can regulate the level of inflammatory factors by affecting the expression of miRNA PC-3p-190-42101 in plasma exosomes to protect the liver from IRI.
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http://dx.doi.org/10.2174/1874467214666211130162152DOI Listing
November 2021

Is motor activity the key to the observation-inflation effect? The role of action simulation.

Mem Cognit 2021 Nov 29. Epub 2021 Nov 29.

School of Psychology, Northeast Normal University, No. 5268 Renmin Street, Changchun, 130024, China.

Observing others performing an action can lead to false memories of self-performance - the observation-inflation effect. The action simulation hypothesis proposes that an action simulation caused by people's observation of an action is the key reason for this effect. Previous studies have inconsistent views of this hypothesis. In the present study, we re-examined the role of action simulation and discussed the key aspects of the mental processes associated with it. We examined the hypotheses that (a) the magnitude of the observation-inflation effect would decrease as the action simulation was impeded and (b) the magnitude of the observation-inflation effect would not be significantly different in conditions in which participants watched either a part of a video or a full video. The results are consistent with the hypotheses. This study provides strong evidence that action simulation influences the generation of observation-inflation effects and that the process is continuous and can refer to further action information.
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http://dx.doi.org/10.3758/s13421-021-01259-xDOI Listing
November 2021

Effect of Moisture Distribution Changes Induced by Different Cooking Temperature on Cooking Quality and Texture Properties of Noodles Made from Whole Tartary Buckwheat.

Foods 2021 Oct 22;10(11). Epub 2021 Oct 22.

Department of Nutrition and Health, China Agricultural University, No.17 Qinghuadonglu, Haidian, Beijing 100083, China.

While precooking and processing have improved the quality of gluten-free noodles, the effects of different cooking temperatures on their quality-neither gluten-free noodles nor whole Tartary buckwheat noodles-have rarely been clarified. This study investigated the key role of moisture distribution induced by different cooking temperatures in improving the noodle quality of whole Tartary buckwheat. The results showed that cooking temperatures higher than 70 °C led to a sharp increase in cooking loss, flavonoid loss and the rate of broken noodles, as well as a sharp decrease in water absorption. Moreover, the noodles cooked at 70 °C showed the lowest rate of hardness and chewiness and the highest tensile strength of all cooking temperatures from 20 °C to 110 °C. The main positive attribute of noodles cooked at 70 °C might be their high uniform moisture distribution during cooking. Cooking at 70 °C for 12 min was determined as the best condition for the quality improvement of whole Tartary buckwheat noodles. This is the first study to illustrate the importance of cooking temperatures on the quality of Tartary buckwheat noodles. More consideration must also be given to the optimal cooking conditions for different gluten-free noodles made from minor coarse cereals.
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http://dx.doi.org/10.3390/foods10112543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625768PMC
October 2021

Sociomoral Temperament: A Mediator Between Wellbeing and Social Outcomes in Young Children.

Front Psychol 2021 8;12:742199. Epub 2021 Nov 8.

Department of Psychology, University of Notre Dame, Notre Dame, IN, United States.

Social outcomes, such as empathy, conscience, and behavioral self-regulation, might require a baseline of psychological wellbeing. According to Triune Ethics Metatheory (TEM), early experience influences the neuropsychology underlying a child's orientation toward the social and moral world. Theoretically, a child's wellbeing, fostered through early caregiving, promotes sociomoral temperaments that correspond to the child's experience, such as social approach or withdrawal in face-to-face situations. These temperaments may represent an individual's default sociomoral perspective on the world. We hypothesized that sociomoral temperament emerges as a function of wellbeing and would be related to social outcomes measured by moral socialization and self-regulation. Further, we hypothesized that sociomoral temperament would mediate the relationship between wellbeing and social outcomes. To investigate, we collected items reflective of sociomoral temperament, asking mothers from two countries (USA: = 525; China: = 379) to report on their 3- to 5-year-old children. They also reported on their child's wellbeing (anxiety, depression, happiness) and social outcomes, including moral socialization (concern after wrong doing, internalized conduct and empathy) and behavioral self-regulation (inhibitory control and misbehavior). As expected, correlations identified connections between wellbeing, sociomoral temperament, and social outcomes. Mediation analyses demonstrated that sociomoral temperament mediated relations between wellbeing and social outcomes in both samples, though in slightly different patterns. Fostering early wellbeing may influence social outcomes through a child's developing sociomoral temperament.
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http://dx.doi.org/10.3389/fpsyg.2021.742199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8606405PMC
November 2021

Screening Novel Drug Candidates for Kidney Renal Clear Cell Carcinoma Treatment: A Study on Differentially Expressed Genes through the Connectivity Map Database.

Kidney Blood Press Res 2021 Oct 11:1-12. Epub 2021 Oct 11.

Department of Urology, Tangshan Central Hospital, Tangshan, China.

Objective: Kidney renal clear cell carcinoma (KIRC) is a common cancer with high morbidity and mortality in renal cancer. Thus, the transcriptome data of KIRC patients in The Cancer Genome Atlas (TCGA) database were analyzed and drug candidates for the treatment of KIRC were explored through the connectivity map (CMap) database.

Methods: The transcriptome data of KIRC patients were downloaded from TCGA database, and KIRC-associated hub genes were screened out through differential analysis and protein-protein interaction (PPI) network analysis. Afterward, the CMap database was used to select drug candidates for KIRC treatment, and the drug-targeted genes were obtained through the STITCH database. A PPI network was constructed by combining drug-targeted genes with hub genes that affected the pathogenesis of KIRC to obtain final hub genes. Finally, combining hub genes and KIRC-associated hub genes, the pathways affected by drugs were explored by pathway enrichment analysis.

Results: A total of 2,312 differentially expressed genes were found in patients, which were concentrated in immune cell activity, cytokine, and chemokine secretion pathways. Drug screening disclosed 5 drug candidates for KIRC treatment: fedratinib, Ly344864, geldanamycin, AS-605240, and luminespib. Based on drug-targeted genes and KIRC-associated hub genes, 16 hub genes were screened out. Pathway enrichment analysis revealed that drugs mainly affected pathways such as neuroactive ligand pathways, cell adhesion, and chemokines.

Conclusion: The above results indicated that fedratinib, LY 344864, geldanamycin, AS-605240, and luminespib could be used as candidates for KIRC therapy. The findings from this study will make contributions to the treatment of KIRC in the future.
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http://dx.doi.org/10.1159/000518437DOI Listing
October 2021

[Determination of the enantiomers of salmeterol xinafoate in salmeterol fluticasone powder inhalant by chiral nonaqueous capillary electrophoresis].

Se Pu 2021 Dec;39(12):1355-1361

Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Institute of Life Science and Green Development, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China.

Salmeterol xinafoate (SalX) is one of the ideal drugs used for the treatment of nocturnal asthma attacks and daily maintenance. The molecular structure of SalX contains a chiral carbon atom, and thus, SalX has two enantiomers, viz. ()-SalX and ()-SalX. It is clinically administered in the racemic form. Related studies have shown that the two enantiomers of SalX are quite different in pharmacology, toxicology, and other aspects. Therefore, it is of great significance to establish an analytical method for the chiral separation and determination of the SalX enantiomers to guarantee their quality and ensure their safety and effectiveness in clinical use. In this study, a chiral nonaqueous capillary electrophoresis (NACE) method, using a L(+)-tartaric acid-boric acid complex as the chiral selector, was established to determine the enantiomers of SalX in salmeterol fluticasone powder inhalant. The L(+)-tartaric acid-boric acid complex was synthesized in situ by the reaction of L(+)-tartaric acid and boric acid in methanol solution. The ion pair principle was considered the enantioseparation mechanism, and the non-aqueous system was found to be more favorable for ion pair formation, which is useful for chiral recognition. Chiral separation is based on the reversible formation of diastereomeric ion pairs between the negatively charged L(+)-tartaric acid-boric acid complex and the positively charged salmeterol enantiomers. Due to the difference in ion-pair binding ability between different enantiomers, the apparent electrophoretic mobilities of different enantiomers were also different, resulting in chiral separation in NACE. To achieve good resolution, the effects of L(+)-tartaric acid concentration, boric acid concentration, and apparent pH (pH ) on the chiral separation were investigated. The optimized buffer solution (pH 0.93) contained 120.0 mmol/L L(+)-tartaric acid and 120.0 mmol/L boric acid in methanol. Other experimental conditions were as follows: uncoated fused-silica capillary with an I. D. of 50.0 μm, a total length () of 64.5 cm, and an effective length () of 55.5 cm, along with gravity injection of 17.5 cm×10.0 s, detection wavelength of 225 nm, room temperature, and operating voltage of 20.0 kV. Under these experimental conditions, the two enantiomers of SalX achieved a resolution of 2.18 within 18.0 min. Both enantiomers showed a good linear relationship of the peak area in the concentration range of 27.5-800.0 mg/L, the correlation coefficient () being greater than 0.9990. The detection limit (=3) and quantitative limit (=10) were 7.5 mg/L and 25.0 mg/L, respectively; the standard recovery was 98.1%-101.9%, with relative standard deviations (RSDs) of 1.2%-1.9%. The intra- and inter-day precisions were examined, and the RSDs of the peak area and migration time were found to be below 4.9% and 1.9%, respectively, indicating good repeatability (inter-day) and reproducibility (inter-day) of the method. The established chiral NACE method was used to determine the two SalX enantiomers in a random salmeterol fluticasone powder inhalant purchased from a local market. The results showed that the percentage of labeled quantities was 98.7% for both enantiomer 1 and enantiomer 2, with RSDs of 2.5% and 2.7%, respectively. Thus, this method is simple, feasible, accurate, and inexpensive, and can be applied for the determination of SalX enantiomers in commercially available salmeterol fluticasone powder inhalants.
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http://dx.doi.org/10.3724/SP.J.1123.2021.06002DOI Listing
December 2021

DGUOK-AS1 acts as a tumorpromoter through regulatingmiR-204-5p/IL-11 axis in breast cancer.

Mol Ther Nucleic Acids 2021 Dec 19;26:1079-1091. Epub 2021 Oct 19.

Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, Wenhua Xi Road No. 107, Jinan, Shandong 250012, P.R. China.

Breast cancer is one of the most lethal malignancies among women; however, the underlying molecular mechanism involved in the progression and metastasis of breast cancer remains unclear. Numerous studies have confirmed that long noncoding RNAs are abnormally expressed in breast cancer and play crucial roles in cell proliferation and metastasis. In the study, we evaluated the functional role and detailed mechanism of DGUOK-AS1 in breast cancer progression and metastasis. DGUOK-AS1 knockdown suppressed proliferation, migration, and invasion of breast cancer cells and . Mechanistically, miR-204-5p was identified as an inhibitory target of DGUOK-AS1, which served as a tumor suppressor in breast cancer. Significantly, we found that the ectopic expression of miR-204-5p could counteract DGUOK-AS1-mediated promotion of cell proliferation and metastasis in breast cancer. Moreover, IL-11 was found to be the downstream target of miR-204-5p, and DGUOK-AS1 could protect IL-11 from miR-204-5p-mediated degradation. DGUOK-AS1 overexpression promoted breast cancer cell migration, angiogenesis, and macrophage migration, mediating by the increased secretion of IL-11, which was extremely important for cancer progression. Collectively, our studies reveal that DGUOK-AS1/miR-204-5p/IL-11 axis plays a significant role in the progression and metastasis of breast cancer, and DGUOK-AS1 might be a novel biomarker and therapeutic target for breast cancer.
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http://dx.doi.org/10.1016/j.omtn.2021.10.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8571540PMC
December 2021

Neutrophil-lymphocyte, platelet-lymphocyte and lymphocyte-monocyte ratios may not be useful markers to assess disease activity in rheumatoid arthritis: A STROBE-compliant article.

Medicine (Baltimore) 2021 Nov;100(45):e27631

Department of Rheumatology, The First People's Hospital of Yibin, Yibin, China.

Abstract: The associations among the neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR) and lymphocyte-monocyte ratio (LMR) and disease activity in rheumatoid arthritis remains unclear.To evaluate these indicators as potential markers of disease activity in patients with rheumatoid arthritis (RA).This cross-sectional study included 547 adult patients with RA. The patients were divided into two groups according to the disease activity score (DAS) system: remission and disease activity. Differences in the NLR, PLR and LMR of the two groups were assessed. Correlations were analyzed using Spearman analysis, and receiver operating characteristic (ROC) curves were used to identify the sensitivity, specificity, and optimal cutoff values to differentiate active RA patients from inactive RA patients.There was a statistically significant difference in the NLR (4.2 ± 3.2 vs 3.4 ± 2.4, P = .034) and PLR (222.3 ± 136.4 vs 176.9 ± 89.8, P = .006) between the two groups, but not for the LMR (3.0 ± 1.8 vs 3.4 ± 2.4, P = .115). In addition, the DAS28 and traditional inflammatory markers, including ESR and CRP, were weakly positively correlated with the NLR and PLR. Based on the ROC curves, the NLR (sensitivity 31.8%, specificity 77.8%) and PLR (sensitivity 57.3%, specificity 63.9%) were less valuable than the ESR (sensitivity 67.2%, specificity 91.7%) and CRP (sensitivity 76.2%, specificity 91.7%) for differentiating inactive RA patients from active RA patients due to low sensitivity and specificity and combining NLR or PLR also cannot significantly improved the diagnostic value of ESR and CRP.NLR, PLR and LMR may not be an useful independent diagnostic or complementary marker for disease activity in RA patients.
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http://dx.doi.org/10.1097/MD.0000000000027631DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589242PMC
November 2021

Prognostic value of preoperative soluble interleukin 2 receptor α as a novel immune biomarker in epithelial ovarian cancer.

Cancer Immunol Immunother 2021 Nov 1. Epub 2021 Nov 1.

Department of Gynecological Oncology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 107 Yanjiang West Road, Guangzhou, People's Republic of China.

Purpose: Epithelial ovarian cancer (EOC) is regarded as the deadliest gynecological cancer, and the demand for novel noninvasive prognostic biomarkers remains significant. This study aimed to investigate the prognostic value of preoperative blood biomarkers in EOC patients.

Methods: In total, 73 patients who had undergone ovarian mass resection were enrolled. Serum concentration of biomarkers, including soluble interleukin 2 receptor α (sIL-2R), was measured 1-2 weeks before surgery. Independent prognostic factors for progression-free survival (PFS) were investigated with multivariate Cox regression analysis. A prognostic model was subsequently developed and evaluated by discrimination, calibration and clinical net benefit. Furthermore, transcriptome data of 376 EOC cases from The Cancer Genome Atlas (TCGA) were analyzed with ESTIMATE, CIBERSORT and Maftools algorithm to evaluate the correlation of IL2RA expression with tumor immune microenvironment and immunotherapeutic response.

Results: High sIL-2R concentration was found to be the only significant prognostic blood biomarker for PFS by multivariate Cox regression analysis in our center. A prognostic nomogram was developed with satisfactory discrimination, calibration and clinical net benefit. In addition, higher IL2RA expression was significantly associated with higher immune scores, activated CD4 T cells, M2 macrophages and resting dendritic cells in TCGA data. Furthermore, IL2RA expression was closely related to TMB scores.

Conclusions: sIL-2R is a potential prognostic immune biomarker for EOC patients, and a comprehensive prognostic model comprising sIL-2R with satisfactory discrimination and clinical appliance was developed. Therefore, we recommend routine sIL-2R testing in EOC patients.
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http://dx.doi.org/10.1007/s00262-021-03092-2DOI Listing
November 2021

Identification and characterization of a thermostable GH11 xylanase from Paenibacillus campinasensis NTU-11 and the distinct roles of its carbohydrate-binding domain and linker sequence.

Colloids Surf B Biointerfaces 2021 Oct 20;209(Pt 1):112167. Epub 2021 Oct 20.

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, 30 Puzhunan Road, Nanjing 211810, Jiangsu, China.

An extracellular thermostable xylanase (XynNTU) from Paenibacillus campinasensis NTU-11, consisted of a glycoside hydrolase (GH) family 11 catalytic domain, a Gly/Pro-rich linker sequence (LS) and a family 6 carbohydrate-binding module (CBM6), was identified and expressed in E. coli BL21. The purified XynNTU had a specific activity of 2750 U/mg and an optimal activity at 60 °C and pH 7.0, and retained a residual activity of 58.4% after incubation (60 °C, 48 h). Two truncated mutants, CBM6-truncated form XynNTU-CDLS, CBM6 and linker-truncated form XynNTU-CD, possessed similar values of optimum pH and temperature as the native XynNTU. XynNTU-CD displayed a lower thermostability than XynNTU, whereas for XynNTU-CDLS, more than 90% of residual activity was remained (60 °C, 48 h), indicating that this enzyme presented a higher thermostability than that of the majority of reported GH11 xylanases. Furthermore, XynNTU and two mutants maintained more than 70% of residual activity at pH values of 5-9. Kinetic measurements suggested that CBM6 had a crucial function in the ability of the enzyme to bind and hydrolyze xylan substrates, while LS had a relatively mild influence. Collectively, a noticeable thermostability and a high specific activity of XynNTU and its truncated form XynNTU-CDLS highlights their potentials for diverse industrial applications.
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http://dx.doi.org/10.1016/j.colsurfb.2021.112167DOI Listing
October 2021

LncRNA SNHG16 accelerates atherosclerosis and promotes ox-LDL-induced VSMC growth via the miRNA-22-3p/HMGB2 axis.

Eur J Pharmacol 2021 Oct 23:174601. Epub 2021 Oct 23.

Department of Cardiovascular Medicine, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China; Key Laboratory of Molecular Cardiology, Xi'an, 710061, Shaanxi, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China. Electronic address:

Long non-coding RNAs (LncRNAs) are essential regulators in the occurrence and development of AS. Here we aim to explore the underlying molecular mechanism of LncRNA SNHG16 in regulating ox-LDL-induced VSMC proliferation, migration and invasion. After constructing AS in vivo and in vitro models, the expressions of SNHG16, miR-22-3p, HMBG2, proliferation- and metastasis-related proteins were determined by qRT-PCR and Western blot assays. Detection of serological lipids, H&E and Masson staining analysis were conducted to evaluate the AS injury in mice. The effects of ox-LDL treatment on VSMCs were examined by CCK-8, wound scratch and Transwell Chamber assays. The targeted relationship was measured by luciferase reporter and RIP assays. The results showed that SNHG16 and high-mobility group box 2 (HMGB2) expressions were increased while miRNA-22-3p expression was decreased in AS mice and ox-LDL-stimulated VSMCs. Functionally, sh-SNHG16 restrained ox-LDL-induced VSMC growth and migration. SNHG16 suppressed miRNA-22-3p expression by direct binding. Furthermore, in ox-LDL-treated VSMCs, miRNA-22-3p mimic prevented proliferation, migration, and invasion. Further explorations showed that HMGB2 was a target of miRNA-22-3p, SNHG16 upregulated HMGB2 levels by acting as a competing endogenous RNA (ceRNA) of miRNA-22-3p. More importantly, sh-HMGB2 partially reversed the effects of sh-SNHG16 together with miR-22-2p inhibitor on ox-LDL-induced VSMC proliferation, migration and invasion. Collectively, SNHG16 accelerated atherosclerotic plaque (AP) formation and enhanced ox-LDL-activated VSMCs proliferation and migration by miRNA-22-3p/HMGB2 axis.
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http://dx.doi.org/10.1016/j.ejphar.2021.174601DOI Listing
October 2021

Dysregulated lncRNA and mRNA may promote the progression of ischemic stroke via immune and inflammatory pathways: results from RNA sequencing and bioinformatics analysis.

Genes Genomics 2021 Oct 26. Epub 2021 Oct 26.

Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education, Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, 130021, China.

Background: Long non-coding RNAs (lncRNAs) are widely involved in gene transcription regulation and which act as epigenetic modifiers in many diseases.

Objective: To determine whether lncRNAs are involved in ischemic stroke (IS), we analyzed the expression profile of lncRNAs and mRNAs in IS.

Methods: RNA sequencing was performed on the blood of three pairs of IS patients and healthy controls. Differential expression analysis was used to identify differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs). Based on the co-expression relationships between lncRNA and mRNA, a series of bioinformatics analysis including GO and KEGG enrichment analysis and PPI analysis, were conducted to predict the function of lncRNA.

Results: RNA sequencing produced a total of 5 DElncRNAs and 144 DEmRNAs. Influenza A pathway and Herpes simplex infection pathway were the most significant pathways. EP300 and NFKB1 were the most important target proteins, and Human leucocyte antigen (HLA) family were the key genes in IS.

Conclusions: Analysis of this study revealed that dysregulated lncRNAs in IS may lead to IS by affecting the immune and inflammation system.
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http://dx.doi.org/10.1007/s13258-021-01173-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546200PMC
October 2021

20 Hz Transcranial Alternating Current Stimulation Inhibits Observation-Execution-Related Motor Cortex Excitability.

J Pers Med 2021 Sep 29;11(10). Epub 2021 Sep 29.

Sports, Exercise and Brain Sciences Laboratory, Beijing Sport University, Beijing 100084, China.

The present study aimed to investigate the effect of transcranial alternating current stimulation (tACS) on the primary motor cortex (M1) during action observation, and subsequent action execution, on motor cortex excitability. The participants received tACS at 10 Hz or 20 Hz, or a sham stimulation over the left M1 for 10 min while they observed a video displaying a repeated button-tapping task using the right hand, and then performed an identical task with their right hand. Motor-evoked potential (MEP) amplitudes were measured before (T0) and after the action observation paired with tACS or a sham stimulation (T1), and after the performance of the action (T2). The results showed that MEPs were significantly reduced at time point T1 ( = 0.042, Cohen's = 0.611) and T2 ( = 0.0003, Cohen's = 0.852) in the 20 Hz tACS condition, in contrast with the sham stimulation. There was a significantly smaller MEP amplitude at time point T2 in the 20 Hz tACS condition, as compared to the 10 Hz tACS condition ( = 0.01, Cohen's = 0.622), but the MEP amplitude did not significantly change at time point T1 between the 20 Hz and 10 Hz tACS conditions ( = 0.136, Cohen's = 0.536). There were no significant differences at time point T1 and T2 between the 10 Hz tACS condition and the sham stimulation. We conclude that 20 Hz tACS during action observation inhibited motor cortex excitability and subsequently inhibited execution-related motor cortex excitability. The effects of tACS on task-related motor cortex excitability are frequency-dependent.
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http://dx.doi.org/10.3390/jpm11100979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8541383PMC
September 2021

Next-generation sequencing of human respiratory syncytial virus subgroups A and B genomes.

J Virol Methods 2022 Jan 18;299:114335. Epub 2021 Oct 18.

Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

Human respiratory syncytial virus (HRSV) is a leading cause of acute respiratory illness in young children worldwide. Whole genome sequencing of HRSV offers enhanced resolution of strain variability for epidemiological surveillance and provides genomic information essential for antiviral and vaccine development. A 10-amplicon one-step RT-PCR assay and a 20-amplicon nested RT-PCR assay with enhanced sensitivity were developed to amplify whole HRSV genomes from samples containing high and low viral loads, respectively. Ninety-six HRSV-positive samples comprised of 58 clinical specimens and 38 virus isolates with C values ≤ 24 were amplified successfully using the 10-amplicon one-step RT-PCR method and multiplexed in a single MiSeq run. Genome coverage exceeded 99.3% for all 96 samples. The 20-amplicon nested RT-PCR NGS method was used to generate >99.6% HRSV full-length genome for 72 clinical specimens with C values ranging from 24 to 33. Phylogenetic analysis of the genome sequences obtained from the 130 clinical specimens revealed a wide diversity of HRSV genotypes demonstrating methodologic robustness.
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http://dx.doi.org/10.1016/j.jviromet.2021.114335DOI Listing
January 2022

Decision tree analysis for evaluating disease activity in patients with rheumatoid arthritis.

J Int Med Res 2021 Oct;49(10):3000605211053232

Molecular Diagnosis Laboratory, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.

Objective: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease characterized by inflammatory synovitis. We developed a new disease activity evaluation system using important cytokines to help doctors better evaluate disease activity in patients with RA.

Methods: Flow cytometry was used to detect the levels of seven cytokines. Then, the results were analyzed using an R language decision tree.

Results: The levels of six cytokines, namely interleukin (IL)-2, IL-4, IL-6, IL-10, tumor necrosis factor-α, and interferon-γ, were significantly different between the active disease and remission stages. Decision tree analysis of the six cytokines with statistical significance identified two judgment rules for the remission stage and three judgment rules for the active disease stage.

Conclusion: We proposed the use of the decision tree method to analyze cytokine levels in patients with RA and obtain a more intuitive and objective RA disease activity scoring system. This method revealed the relationships of IL-6 and TNF-α levels with inflammatory characteristics in patients with RA, which can help predict disease activity.
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http://dx.doi.org/10.1177/03000605211053232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8543724PMC
October 2021

Knockdown of TRIM11 suppresses cell progression and apoptosis of cervical cancer cells via PI3K/AKT pathway.

Am J Transl Res 2021 15;13(9):10328-10340. Epub 2021 Sep 15.

Gynecology Ward, Jinan Maternity and Child Care Hospital Jinan, Shandong Province, China.

Objective: To detect the expression pattern of TRIM11 in CC and to investigate the effect of TRIM11 knockdown on CC progression.

Methods: First, the expression pattern and function of TRIM11 in CC were inferred by GEPIA database. The expression of TRIM11 in CC tissues and cells was verified by RT-qPCR and Western blot assays. TRIM11 shRNA was transfected into CC cells. The effect of TRIM11 knockdown on CC cell proliferation and cell apoptosis was assessed by CCK-8 assay, clone formation test and flow cytometry (FCM). Furthermore, the effect of TRIM11 knockdown on cell migration and invasion was measured by Transwell assay. The apoptosis-related proteins (Bax, Bcl-2 and Cleaved caspase 3) and PI3K/AKT pathway-related proteins (PI3K, p-PI3K, AKT and p-Akt) were examined by Western blot assay.

Results: TRIM11 was found to be dramatically upregulated in CC tissues and cells. Besides this, TRIM11 was closely related to FIGO stage, infiltration depth and metastasis. Moreover, high expression of TRIM11 was found to be associated with poor prognosis of CC patients. Functional experiments showed that knockdown of TRIM11 obviously suppressed CC cell proliferation, migration and invasion, while accelerated cell apoptosis. In addition, the PI3K inhibitor (LY294002) was used to assess the connection between PI3K/AKT pathway and TRIM11 in CC cells. We found that TRIM11 overexpression suppressed the phosphorylation of PI3K and AKT in CC cells.

Conclusion: Hence, TRIM11 regulates CC cell progression by modulating PI3K/AKT pathway. The results suggested that TRIM11 might be a possible target for the diagnosis and prognosis of CC patients.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507035PMC
September 2021

[Genetic analysis of a family with 9q34.3 microdeletion and microduplication caused by abnormal chromosome balance structure].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Oct;38(10):961-965

Forensic Evidence Laboratory, Shenzhen People's Hospital (The Second Clinical Medical College,Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong 518020, China.

Objective: To perform prenatal diagnosis, pedigree analysis, and genetic counseling of a pregnant woman who gave birth to a child with Kleefstra syndrome.

Methods: Karyotype analysis, chromosomal microarray analysis (CMA), multiplex ligation-dependent probe amplification (MLPA) and fluorescence in situ hybridization (FISH) were used of peripheral blood and amniotic fluid to find causes. Recurrence risk assessment was performed later.

Results: The amniotic fluid sample showed a 9q34.3 microduplication of arr (hg19) 9q34.3 (140 168 806-141 020 389)× 3, which overlapped the 9q34.3 microdeletion region of proband. The pregnant woman was detected with a balanced translocation of ish, t(9;17)(9q34.3; qter) (9p+; 17p+,9q+, 17q+). No other abnormal results were found in the family.

Conclusion: Offspring who share the same chromosome segment deletion or duplication are always from parent who carries balanced chromosomal structural aberration.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200413-00259DOI Listing
October 2021

Association of Preoperative Serum Levels of CEA and CA15-3 with Molecular Subtypes of Breast Cancer.

Dis Markers 2021 27;2021:5529106. Epub 2021 Sep 27.

Pathology Tissue Bank, Qilu Hospital of Shandong University, Jinan, Shandong, China.

Objectives: Molecular subtypes are employed as a guide for targeted treatment and important prognostic factors. This study focused on investigating the association of serum levels of CEA, CA15-3, and CA125 with clinicopathological characteristics of breast cancer to find prognostic markers for breast cancer and provide precise targeted therapy.

Materials And Methods: In this study, 961 breast cancer patients with preoperative serum levels of CEA, CA15-3, and CA125 and molecular subtypes were analyzed. Cut-off values of 5 ng/ml, 25 U/ml, and 35 U/ml were used for CEA, CA15-3, and CA125, respectively. The test and Fisher exact test along with logistic multivariate regression analysis were performed for investigating the correlation of CEA, CA15-3, and CA125 serum levels with molecular subtypes and associated factors.

Results: An increase in the serum concentrations of CEA, CA15-3, and CA125 was discovered in 48 (4.99%), 54 (5.62%), and 55 (5.72%) breast cancer patients, respectively. Univariate analysis demonstrated that the levels of CEA ( < 0.01) and CA15-3 ( < 0.05) were significantly linked with molecular types of breast cancer. Moreover, patients having larger tumor size ( < 0.01, < 0.0001, and < 0.05, respectively) along with nodal metastasis ( < 0.05, = 0.0001, and < 0.05, respectively) exhibited higher rates of elevated CEA, CA15-3, and CA125 levels. Status of Her-2 positive ( < 0.01) had a significant connection with elevated CEA levels. Multivariate analysis further indicated that molecular subtypes were independent factors associated with CEA and CA15-3 levels. Also, Her-2 status was significantly and independently related to CEA levels.

Conclusion: Preoperative serum levels of CEA and CA15-3 were independently associated with molecular subtypes of breast cancer. CEA and CA15-3 might improve the prognostic prediction for patients with breast cancer and inform the selection of specific therapies. A further biological analysis is needed for investigating the relationship between Her-2 expression and CEA levels.
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http://dx.doi.org/10.1155/2021/5529106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8492280PMC
September 2021

Prevalence and correlates of muscle-strengthening exercise among Chinese children and adolescents: Results from 2019 Physical Activity and Fitness in China-The Youth Study.

J Sport Health Sci 2021 Oct 1. Epub 2021 Oct 1.

School of Physical Education and Sport Training, Shanghai University of Sport, Shanghai 200438, China. Electronic address:

Background: To identify the prevalence of meeting the muscle-strengthening exercise (MSE) recommendations and its correlates among Chinese children and adolescents.

Methods: Cross-sectional data from 80,413 Chinese children and adolescents (mean age = 13.7 years; 53.9% girls) and their parents were analyzed. Self-reported data on MSE, demographics (sex, grade, ethnicity, residence location, height, weight, family composition and income, and parental education level), behavior (sport skills, moderate-to-vigorous physical activity, screen time, and sleep duration), psychology (exercise intention), and sociocultural background (peer and parental support and parental MSE) were obtained. Logistic regression models were used to determine the correlates of meeting the recommendations.

Results: Overall, 39.3% of children and adolescents met the MSE recommendations. At the demographic level, girls, 10th-12th graders, those who were overweight or obese, minorities, and those with lower income and lower parental education levels were less likely to meet the MSE recommendations. At the behavioral level, children proficient in ≥ 2 sport skills were more likely to meet the MSE recommendations (odds ratio [OR] = 1.44, 95% confidence interval [CI] 95%CI: 1.26-1.66), as were those with more moderate-to-vigorous physical activity (OR = 1.57, 95%CI: 1.53-1.61). At the psychological level, children with high exercise intention were more likely to meet the MSE recommendations (OR = 1.59, 95%CI: 1.51-1.68). At the sociocultural level, children whose parents met the adult MSE recommendations (OR = 1.46, 95%CI: 1.40-1.53) and who received high peer (OR = 1.27, 95%CI: 1.20-1.35) and parental support (OR = 1.08, 95%CI: 1.03-1.12) had a greater chance of meeting the MSE recommendations.

Conclusion: Less than two-fifths of Chinese children and adolescents met the MSE recommendations. Sex, age, weight status, ethnicity, family income, parental education level, sport skills, moderate-to-vigorous physical activity, exercise intention, peer and parental support, and parental MSE were significantly associated with meeting the recommendations.
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http://dx.doi.org/10.1016/j.jshs.2021.09.010DOI Listing
October 2021

Comprehensive Analysis of RNA Expression Profile Identifies Hub miRNA-circRNA Interaction Networks in the Hypoxic Ischemic Encephalopathy.

Comput Math Methods Med 2021 21;2021:6015473. Epub 2021 Sep 21.

Department of Neonatology, Jinan Maternal and Child Health Care Hospital, Shandong, China.

Hypoxic ischemic encephalopathy (HIE) is classified as a sort of serious nervous system syndrome that occurs in the early life period. Noncoding RNAs had been confirmed to have crucial roles in human diseases. So far, there were few systematical and comprehensive studies towards the expression profile of RNAs in the brain after hypoxia ischemia. In this study, 31 differentially expressed microRNAs (miRNAs) with upregulation were identified. In addition, 5512 differentially expressed mRNAs, long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs) were identified in HIE groups. Bioinformatics analysis showed these circRNAs and mRNAs were significantly enriched in regulation of leukocyte activation, response to virus, and neutrophil degranulation. Pathway and its related gene network analysis indicated that HLA - DPA1, HLA - DQA2, HLA - DQB1, and HLA - DRB4 have a more crucial role in HIE. Finally, miRNA-circRNA-mRNA interaction network analysis was also performed to identify hub miRNAs and circRNAs. We found that miR-592 potentially targeting 5 circRNAs, thus affecting 15 mRNA expressions in HIR. hsa_circ_0068397 and hsa_circ_0045698 were identified as hub circRNAs in HIE. Collectively, using RNA-seq, bioinformatics analysis, and circRNA/miRNA interaction prediction, we systematically investigated the differentially expressed RNAs in HIE, which could give a new hint of understanding the pathogenesis of HIE.
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http://dx.doi.org/10.1155/2021/6015473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481051PMC
September 2021

Sequence- and structure-guided improvement of the catalytic performance of a GH11 family xylanase from Bacillus subtilis.

J Biol Chem 2021 Nov 1;297(5):101262. Epub 2021 Oct 1.

School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Brisbane, Queensland, Australia.

Xylanases produce xylooligosaccharides from xylan and have thus attracted increasing attention for their usefulness in industrial applications. Previously, we demonstrated that the GH11 xylanase XynLC9 from Bacillus subtilis formed xylobiose and xylotriose as the major products with negligible production of xylose when digesting corncob-extracted xylan. Here, we aimed to improve the catalytic performance of XynLC9 via protein engineering. Based on the sequence and structural comparisons of XynLC9 with the xylanases Xyn2 from Trichoderma reesei and Xyn11A from Thermobifida fusca, we identified the N-terminal residues 5-YWQN-8 in XynLC9 as engineering hotspots and subjected this sequence to site saturation and iterative mutagenesis. The mutants W6F/Q7H and N8Y possessed a 2.6- and 1.8-fold higher catalytic activity than XynLC9, respectively, and both mutants were also more thermostable. Kinetic measurements suggested that W6F/Q7H and N8Y had lower substrate affinity, but a higher turnover rate (k), which resulted in increased catalytic efficiency than WT XynLC9. Furthermore, the W6F/Q7H mutant displayed a 160% increase in the yield of xylooligosaccharides from corncob-extracted xylan. Molecular dynamics simulations revealed that the W6F/Q7H and N8Y mutations led to an enlarged volume and surface area of the active site cleft, which provided more space for substrate entry and product release and thus accelerated the catalytic activity of the enzyme. The molecular evolution approach adopted in this study provides the design of a library of sequences that captures functional diversity in a limited number of protein variants.
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http://dx.doi.org/10.1016/j.jbc.2021.101262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8546418PMC
November 2021

Iron Status Among Children Aged 6-17 Years by Serum Ferritin - China, 2016-2017.

China CDC Wkly 2021 Mar;3(11):221-225

National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Key Laboratory of Trace Element Nutrition, National Health Commission of the People's Republic of China, Beijing, China.

What Is Already Known On This Topic?: Iron deficiency (ID) is the most widespread micronutrient deficiency and have several adverse effects on health. Consequences of ID among children include delayed psychomotor development and impaired cognitive performance, which makes it important to monitor the iron status of children.

What Is Added By This Report?: In this study, the serum ferritin (SF) level was 56.6 (95% CI: 56.0-57.2) ng/mL in 65,293 children aged 6-17 years old in the National Nutrition and Health Surveillance in China in 2016-2017. ID prevalence varied significantly in children stratified by sex, age, and regions ranging from 1.0% to 28.1% judged by the standard of SF<15 ng/mL and SF<25 ng/mL. ID prevalence in females aged 12-17 years was the highest among children aged 6-17 years.

What Are The Implications For Public Health Practice?: Understanding iron status of school children could provide evidence and data for developing policies and strategies for ID and iron deficiency anemia (IDA) control and prevention. Females aged 12-17 years showed high ID prevalence, and iron-rich food interventions are strongly recommended.
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http://dx.doi.org/10.46234/ccdcw2021.063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8393040PMC
March 2021

Population Performance of Thrips hawaiiensis (Thysanoptera: Thripidae) on Different Vegetable Host Plants.

Neotrop Entomol 2021 Dec 29;50(6):923-928. Epub 2021 Sep 29.

Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, Guizhou Provincial Engineering Research Center for Biological Resources Protection and Efficient Utilization of the Mountainous Region, Department of Biology and Engineering of Environment, Guiyang University, Guiyang, China.

Thrips hawaiiensis (Morgan) is a flower-inhabiting thrips with a wide range of host plants, but little is known regarding its biological and ecological characteristics on vegetable hosts. Here, we evaluated the development, survival, and oviposition of T. hawaiiensis on five vegetable species (Capsicum annuum, Solanum melongena, Cucurbita moschata, Lablab purpureus, and Brassica oleracea), and constructed its life tables on these vegetables. There were significant differences in the development of T. hawaiiensis on the five vegetables, and the developmental times from egg to adult were 12.19 days, 11.59 days, 11.12 days, 10.78 days, and 10.51 days on C. moschata, B. oleracea, L. purpureus, C. annuum, and S. melongena, respectively. There were also significant differences in T. hawaiiensis' survival rate on these plants, with S. melongena (71.00%) > C. annuum (67.33%) > L. purpureus (63.33%) > B. oleracea (57.00%) > C. moschata (49.33%). The greatest and lowest fecundity levels of T. hawaiiensis were found on S. melongena (44.28) and C. moschata (30.16), respectively. T. hawaiiensis had the greatest net reproductive rate on S. melongena (19.22), followed by C. annuum (16.11), L. purpureus (15.17), B. oleracea (11.10), and C. moschata (8.47), and the intrinsic rate of increase showed a similar trend, with values of 0.140, 0.125, 0.121, 0.112, and 0.093, respectively. Thus, S. melongena and C. moschata were the most and least suitable hosts for the population development of T. hawaiiensis among the five tested vegetable hosts. This study could provide important information for the key control of T. hawaiiensis on different crops.
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http://dx.doi.org/10.1007/s13744-021-00914-xDOI Listing
December 2021

Fabricating an anti-shrinking κ-carrageenan/sodium carboxymethyl starch film by incorporating carboxylated cellulose nanofibrils for fruit preservation.

Int J Biol Macromol 2021 Nov 25;191:706-713. Epub 2021 Sep 25.

Key Laboratory of Bio-based Materials Science and Technology of Ministry of Education, Northeast Forestry University, 26th Hexing Road, Xiangfang District, Harbin 150040, PR China. Electronic address:

A stronger and dimension-stabilized film was obtained using κ-carrageenan and sodium carboxymethyl starch (CMS) with carboxylated cellulose nanocrystals (C-CNC) as a reinforcing agent and anti-shrinkage agent. C-CNC endowed the films with better mechanical properties as well as excellent dimensional stability. The film solutions showed shear thinning and acted as a pseudoplastic fluid. When C-CNC content was increased from 0% to 12%, the tensile strength and elongation at break of the films improved from 23.89 MPa to 38.37 MPa and 21.00% to 27.31%, respectively. The films maintained good thermal stability and barrier performance. The Zeta potential of the film suspension can reach below -30 mV, indicating C-CNC enhanced the electrostatic repulsion in the film-forming system, which favored the network structure more continuous and stable. By virtue of the excellent mechanical properties and dimensional stability, strawberries can be tightly wrapped without cracks by the coatings to delay the deterioration greatly. By comparing the weight loss rate, Vc, total soluble solid, hardness, titratable acid and pH, CCC12-coated strawberries were closer to fresh ones. Therefore, this study has developed a feasible, low-cost and green fruit coating that can be potentially utilized on a large-scale.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.09.134DOI Listing
November 2021

Association of O blood type with the prognosis of acute necrotizing encephalopathy in childhood: A single-center cohort study.

Brain Dev 2021 Sep 22. Epub 2021 Sep 22.

Pediatric Intensive Care Unit, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.

Background: Acute necrotizing encephalopathy (ANE) of childhood is a rare but critical disease with global distribution. Few studies have focused on investigating the relationship between O blood type and the prognosis of ANE.

Methods: We analyzed retrospectively the data of patients with ANE admitted to the Beijing Children's Hospital from March 2012 to February 2019. Baseline data, clinical characteristics, examination, treatment, and prognosis of O blood group were compared with those of the non-O blood group. Cox regression was used to observe the independent prognostic factors in ANE.

Results: Thirty-one ANE patients were recruited, and 8 (25.8%) of these patients were in the O blood group. The rest (n = 23; 74.2%) were in the non-O blood group. No significant differences were found in the demographic characteristics, clinical features, examinations, and treatments between the two groups (p > 0.05). At 28 days after discharge, the overall survival rate of the O blood group was significantly higher than that of the non-O blood group (χ = 5.630, p = 0.018). At 1 year after discharge, the survival quality of the O blood group was higher than that of the non-O blood group (p = 0.006). After adjusting for the confounding factors, Cox regression analysis showed that O blood type might be a protective factor in ANE [hazard ratio = 0.283, 95% CI = 0.081-0.988; p = 0.048].

Conclusions: O blood type may be a protective factor for patients with ANE.
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http://dx.doi.org/10.1016/j.braindev.2021.09.004DOI Listing
September 2021

The clinical effects of high-quality nursing interventions after a diagnosis of upper gastrointestinal bulging lesions with mEUS.

Am J Transl Res 2021 15;13(8):9655-9662. Epub 2021 Aug 15.

Ultrasound Department, Linyi People Hospital Linyi, China.

Purpose: To study and analyze the clinical effectiveness of miniprobe endoscopic ultrasonography (mEUS) in upper gastrointestinal eminence lesions caused by tumors.

Methods: We recruited 210 patients admitted to our hospital from March 2018 to June 2020 as the study cohort, all of whom were diagnosed with upper gastrointestinal eminence lesions caused by tumors using routine endoscopies. To further clarify the type of disease, mEUS was used in all the patients along with high-quality nursing interventions. The results and the patients' anxiety and depression levels before and after the examinations were analyzed.

Results: After their admission, the patients' SAS and SDS scores were high. Their scores decreased significantly after the nursing (P < 0.05). There were 75 patients with esophageal disease diagnosed among the 223 patients with upper gastrointestinal eminence lesions, accounting for 33.63% of the total. 114 patients with gastric disease were found, accounting for 51.12%; and there were 5 cases of cardia disease, accounting for 2.24% of the total. Another 29 cases of duodenal disease were detected, accounting for 13%. According to the determinations of the pathological types, the main disease types were stromal tumors and leiomyomas, with 79 cases (35.43%) and 53 cases (23.77%). The mEUS examinations in the 210 patients detected a total of 223 eminence lesions. In total, the 213 lesions were consistent with the pathological examinations, and the total diagnosis coincidence rate was 95.52% (213/223).

Conclusion: mEUS examinations combined with nursing care applied to tumor-induced upper gastrointestinal swelling lesions can eliminate patient tension and anxiety and other unhealthy emotions and can improve patient compliance with the examinations. mEUS examinations can show the hierarchical structure of the upper gastrointestinal wall and accurately locate any upper gastrointestinal swelling lesions, effectively clarify the nature of the disease, and have a guiding significance for the differential diagnosis and treatment of the disease.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430168PMC
August 2021
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