Publications by authors named "Lijuan Tan"

10 Publications

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The Value of Circulating microRNAs for Diagnosis and Prediction of Preeclampsia: a Meta-analysis and Systematic Review.

Reprod Sci 2021 Nov 24. Epub 2021 Nov 24.

Department of Medical Genetic, The Affiliated Hospital of Qingdao University, 16 Jiangsu Road, Qingdao, 266003, China.

Preeclampsia (PE) is one of the main causes of maternal death worldwide, but our understanding of the molecular characteristics of disease progression is limited. In this meta-analysis, we aimed to assess the value of peripheral blood microRNAs (miRNAs) as diagnostic and predictive markers of PE. We screened PubMed, Web of Science, and Embase databases; searched articles about "miRNAs and PE" up to November 30, 2020; and conducted biological information and subgroup analysis. We used QUADAS-2 (quality assessment of diagnostic accuracy studies-2) to evaluate the included articles by two independent reviewers, calculated the combined diagnostic and predictive indicators using the random effects model, explored the sources of potential heterogeneity through subgroup analysis, and evaluated publication bias using Deeks' funnel plot asymmetry test using Stata 14.0 and Review Manager 5.3 software. Forty-three miRNAs from 15 studies, including 2042 healthy controls and 2685 PE patients, had a pooled sensitivity of 0.86 (95% CI: 0.81-0.90), specificity of 0.89 (95% CI: 0.85-0.92), and an AUC of 0.94 (95% CI: 0.91-0.96). Moreover, before 20 weeks of gestation, the combined sensitivity was 0.86 (95% CI: 0.75-0.92), and the specificity was 0.90 (95% CI: 0.83-0.95), which indicated that some of the circulating miRNAs had changed significantly before the clinical symptoms appeared in PE patients. Circulating miRNAs have high diagnostic and predictive accuracy and may be used as non-invasive biomarkers for the diagnosis and prediction of PE. However, a large sample prospective study is still needed.
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http://dx.doi.org/10.1007/s43032-021-00799-6DOI Listing
November 2021

Serum lactate dehydrogenase activities as systems biomarkers for 48 types of human diseases.

Sci Rep 2021 06 21;11(1):12997. Epub 2021 Jun 21.

Department of Cardiology, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China.

Most human diseases are systems diseases, and systems biomarkers are better fitted for diagnostic, prognostic, and treatment monitoring purposes. To search for systems biomarker candidates, lactate dehydrogenase (LDH), a housekeeping protein expressed in all living cells, was investigated. To this end, we analyzed the serum LDH activities from 172,933 patients with 48 clinically defined diseases and 9528 healthy individuals. Based on the median values, we found that 46 out of 48 diseases, leading by acute myocardial infarction, had significantly increased (p < 0.001), whereas gout and cerebral ischemia had significantly decreased (p < 0.001) serum LDH activities compared to the healthy control. Remarkably, hepatic encephalopathy and lung fibrosis had the highest AUCs (0.89, 0.80), sensitivities (0.73, 0.56), and specificities (0.90, 0.91) among 48 human diseases. Statistical analysis revealed that over-downregulation of serum LDH activities was associated with blood-related cancers and diseases. LDH activities were potential systems biomarker candidates (AUCs > 0.8) for hepatic encephalopathy and lung fibrosis.
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http://dx.doi.org/10.1038/s41598-021-92430-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217520PMC
June 2021

Diagnostic and Prognostic Biomarkers for Myocardial Infarction.

Front Cardiovasc Med 2020 3;7:617277. Epub 2021 Feb 3.

Systems Biology & Medicine Center for Complex Diseases, Center for Clinical Research, Affiliated Hospital of Qingdao University, Qingdao, China.

The incidence of myocardial infarction (MI) increases every year worldwide. Better diagnostic and prognostic biomarkers for clinical applications are the consistent pursuit of MI research. In addition to electrocardiogram, echocardiography, coronary angiography, etc., circulating biomarkers are essential for the diagnosis, prognosis, and treatment effect monitoring of MI patients. In this review, we assessed both strength and weakness of MI circulating biomarkers including: (1) originated from damaged myocardial tissues including current golden standard cardiac troponin, (2) released from non-myocardial tissues due to MI-induced systems reactions, and (3) preexisted in blood circulation before the occurrence of MI event. We also summarized newly reported MI biomarkers. We proposed that the biomarkers preexisting in blood circulation before MI incidents should be emphasized in research and development for MI prevention in near future.
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http://dx.doi.org/10.3389/fcvm.2020.617277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7886815PMC
February 2021

Prognostic value of serum calprotectin level in elderly diabetic patients with acute coronary syndrome undergoing percutaneous coronary intervention: A Cohort study.

Medicine (Baltimore) 2020 Aug;99(33):e20805

From the Department of Critical Care Medicine, Shanxi Cardiovascular Hospital, Taiyuan 030024, Shanxi, China.

Patients with acute coronary syndrome (ACS) have an increased serum level of calprotectin. The purpose of present study was to analyze the prognostic significance of serum calprotectin levels in elderly diabetic patients underwent percutaneous coronary intervention (PCI) due to ACS.A total of 273 consecutive elderly diabetic patients underwent PCI for primary ACS were enrolled. Serum calprotectin levels were measured before PCI, and baseline clinical characteristics of all patients were collected. All patients were followed up at regular interval for major adverse cardiovascular events (MACEs) during 1 year after PCI. MACEs include cardiovascular death, nonfatal myocardial infarction, and target vessel revascularization (TVR). The predicting value of serum calprotectin for MACEs was analyzed by using univariate and multivariate analysis and receiver-operating characteristic curve (ROC).At the endpoint of this study, 47 patients of all 273 patients had MACEs. According to optimal cutoff value of calprotectin for predicting MACEs by ROC analysis, all patients were stratified into a high calprotectin group and a low calprotectin group. The incidence rate of MACEs and TVR in high calprotectin group was prominently higher than that in low calprotectin group (21.9% vs 11.5%, P = .02). In multivariable COX regression analysis adjusting for potential confounders, serum calprotectin level remains as an independent risk predictor of MACE (hazard ratio, 1.56; 95% confidence interval [CI]: 1.08-4.62; P = .01).In diabetic patients with a comorbidity of ACS, a high serum level of calprotectin is associated to a higher MACE rate after PCI.
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http://dx.doi.org/10.1097/MD.0000000000020805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7437750PMC
August 2020

Quantum random number generator based on single-photon emitter in gallium nitride.

Opt Lett 2020 Aug;45(15):4224-4227

We experimentally demonstrate a real-time quantum random number generator by using a room-temperature single-photon emitter from the defect in a commercial gallium nitride wafer. Due to the brightness of our single-photon emitter, the raw bit generation rate is about 1.8 MHz, and the unbiased bit generation rate is about 420 kHz after the von Neumann's randomness extraction procedure. Our results show that the commercial gallium nitride wafer has great potential for the development of integrated high-speed quantum random number generator devices.
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http://dx.doi.org/10.1364/OL.396561DOI Listing
August 2020

[Cloning and expression of duck C4BPα and verification of its interaction with Riemerella anatipestifer].

Sheng Wu Gong Cheng Xue Bao 2020 Apr;36(4):693-699

College of Animal Science, Southwest University, Chongqing 402460, China.

To study the interaction between C4b-binding protein (C4BP) and Riemerella anatipestifer (RA), we cloned duck C4BPα, conducted prokaryotic expression and prepared the polyclonal antibody by immunizing mice. Then indirect immunofluorescence assay and dot blotting hybridization assay were used to verify the interaction between C4BP and RA. The full length of duck C4BPα nucleotide sequence was 1 230 bp, with the highest similarity to chicken C4BPα (82.1%). Phylogenetic tree analysis showed that duck C4BPα and chicken C4BPα were on the same phylogenetic tree branch and the genetic evolution relationship between them was the closest. C4BPα was efficiently expressed in Escherichia coli BL21 (DE3). The recombinant proteins existed in intracellular soluble form. The titer of polyclonal antibody was more than 1:10 000 and polyclonal antibodies could specifically recognize the recombinant proteins. The results of indirect immunofluorescence assay and dot blot hybridization assay showed that RA could interact with duck C4BP. The results provide a basis to further reveal the pathogenesis of RA.
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http://dx.doi.org/10.13345/j.cjb.190234DOI Listing
April 2020

[Cloning and expression of duck C4BPα and verification of its interaction with Riemerella anatipestifer].

Sheng Wu Gong Cheng Xue Bao 2020 Apr;36(4):693-699

College of Animal Science, Southwest University, Chongqing 402460, China.

To study the interaction between C4b-binding protein (C4BP) and Riemerella anatipestifer (RA), we cloned duck C4BPα, conducted prokaryotic expression and prepared the polyclonal antibody by immunizing mice. Then indirect immunofluorescence assay and dot blotting hybridization assay were used to verify the interaction between C4BP and RA. The full length of duck C4BPα nucleotide sequence was 1 230 bp, with the highest similarity to chicken C4BPα (82.1%). Phylogenetic tree analysis showed that duck C4BPα and chicken C4BPα were on the same phylogenetic tree branch and the genetic evolution relationship between them was the closest. C4BPα was efficiently expressed in Escherichia coli BL21 (DE3). The recombinant proteins existed in intracellular soluble form. The titer of polyclonal antibody was more than 1:10 000 and polyclonal antibodies could specifically recognize the recombinant proteins. The results of indirect immunofluorescence assay and dot blot hybridization assay showed that RA could interact with duck C4BP. The results provide a basis to further reveal the pathogenesis of RA.
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http://dx.doi.org/10.13345/j.cjb.190234DOI Listing
April 2020

Comparison of Ticagrelor and Clopidogrel for Patients Undergoing Emergency Percutaneous Coronary Intervention.

Iran J Public Health 2018 Jul;47(7):952-957

Dept. of Cardiology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003 China.

Background: We compared treatments with the antiplatelets ticagrelor and clopidogrel used in patients with acute myocardial infarction (AMI) during the perioperative period for emergency percutaneous coronary intervention (PCI).

Methods: A total of 120 patients were selected and randomly divided into control and observation groups (60 patients in each) from 2014-2016 at The Affiliated Hospital of Qingdao University. The patients in the control group received 300 mg clopidogrel and 300 mg aspirin for oral administration, while those in the observation group were given 180 mg ticagrelor and 300 mg aspirin orally prior to the PCI. During the operation, heparinization and a tirofiban micro-pump were used continuously.

Results: Coronary artery and peripheral venous blood were extracted from each patient to obtain various parameters of thrombelastogram (TEG), and the maximum platelet aggregation rates in order to compare antiplatelet effects. Major adverse cardiac events (MACE) were recorded during the following 6-month follow-up. Analysis of the data showed no differences in terms of the time span between medication intake and stent implantation, or the dosage of heparin and tirofiban used between the two groups. Before stent implantation, and 24 and 48 h after the procedure the average R and K values of TEG in coronary artery blood and peripheral venous blood samples in the observation group were longer than those in the control group, while the α angle, MA, CI, MARAA and MARADP values were lower (<0.05).

Conclusion: Ticagrelor can improve antiplatelet treatment for patients with AMI during the perioperative period of emergency PCI.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6119568PMC
July 2018

Baicalin promotes cholesterol efflux by regulating the expression of SR-BI in macrophages.

Exp Ther Med 2016 Dec 8;12(6):4113-4120. Epub 2016 Nov 8.

Department of Cardiology, Central Hospital of Zibo, Zibo, Shandong 255036, P.R. China.

Intake of a high dosage of baicalin has previously been shown to attenuate hyperlipidemia induced by a high-fat diet. Baicalin functions as an activator of peroxisome proliferator-activated receptor-γ (PPAR-γ), which is the key regulator of reverse cholesterol transport (RCT). The present study aimed to test the hypothesis that baicalin could promote cholesterol efflux in macrophages through activating PPAR-γ. Phorbol 12-myristate 13-acetate-stimulated THP-1 cells were treated with oxidized low-density lipoprotein and (H)-cholesterol for 24 h, and the effects of baicalin on cholesterol efflux were evaluated in the presence of apolipoprotein A-1 (ApoA-1), or high-density lipoprotein subfraction 2 (HDL) or subfraction 3 (HDL). The expression levels of scavenger receptor class B type I (SR-BI), PPAR-γ and liver X receptor-α (LXRα) were detected and specific inhibitors or activators of SR-BI, PPAR-γ and LXRα were applied to investigate the mechanism. Treatment of THP-1 macrophages with baicalin significantly accelerated HDL-mediated, but not ApoA-1-mediated cholesterol efflux. However, baicalin treatment increased the expression of SR-BI at the mRNA and protein levels in a dose- and time-dependent manner, and pre-treatment with the SR-BI inhibitor BLT-1 and SR-BI small interfering RNA significantly inhibited baicalin-induced cholesterol efflux. Furthermore, baicalin increased the expression of PPAR-γ and LXRα, and the application of specific agonists and inhibitors of PPAR-γ and LXRα changed the expression of SR-BI, as well as cholesterol efflux. It may be concluded that baicalin induced cholesterol efflux from THP-1 macrophages via the PPAR-γ/LXRα/SR-BI pathway.
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http://dx.doi.org/10.3892/etm.2016.3884DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5228540PMC
December 2016

The Effects of Fasudil at Different Doses on Acute Myocardial Infarction in Rats.

Acta Cardiol Sin 2013 Nov;29(6):524-30

Department of Cardiololgy, Huangdao Branch, Affiliated Hospital of Qingdao University Medical College, Qing Dao, Shan Dong, China, 266555.

Purpose: To explore the effects of different doses of fasudil on cardiomyocytes in rats with acute myocardial infarction (AMI).

Methods: A model of rats experiencing AMI was randomly divided into control groups and fasudil treatment groups according to the different doses of fasudil. After four weeks, hemodynamic parameters were measured. Expression levels of Rho kinase mRNA by the reverse transcription polymerase chain reaction method and the expression levels of apoptosis related proteins, Bcl-2 and bax, were determined by the immunohistochemical method.

Results: In the model of AMI in rats, their hemodynamic deteriorated, and the expression level of the Rho kinase mRNA increased in the myocardial tissue; but the expression level of apoptosis-related protein bcl-2 decreased, and Bax increased (p < 0.01). After the administration of fasudil, hemodynamic levels improved (p < 0.05), expression levels of Rho kinase mRNA and Bax (p < 0.01) decreased, expression levels of bcl-2 increased, and with the added element of dosage increase, the effect was significant (p < 0.05).

Conclusions: By administration of different doses of fasudil, the expression level of Rho kinase in myocardial tissue decreased and apoptosis reduced in rats with AMI. Fasudil plays an important role in protecting ischemic myocardium and improving cardiac function post AMI in rats, the effects of which were enhanced as the dosage was increased.

Key Words: Apoptosis; Myocardial infarction; Rho kinase.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4805031PMC
November 2013
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