Publications by authors named "Lihua Zhao"

124 Publications

Molecular characterization and functional analysis of Trx and Trp14 in roughskin sculpin (Trachidermus fasciatus).

Fish Physiol Biochem 2021 Jul 19. Epub 2021 Jul 19.

Marine College, Shandong University, Weihai, Shandong, 264209, China.

Thioredoxins (Trxs) are a family of small and highly conserved proteins which play crucial roles in the maintenance and regulation of the cellular redox homeostasis. In this study, the full-length cDNAs of thioredoxin 1 (TfTrx1) and thioredoxin-related protein of 14 kDa (TfTrp14) were isolated from roughskin sculpin (Trachidermus fasciatus). TfTrx1 is 662 bp in length with a 336-bp open reading frame (ORF) that encodes for a peptide with 111 amino acids, and TfTrp14 consists of 1066 bp with a 372-bp ORF that is translated to 123 amino acids. TfTrx1 and TfTrp14 contain highly conserved catalytic site motif CGPC and CPDC, respectively. Tissue distribution analysis indicated that both genes were broadly expressed in all examined tissues with the highest expression of TfTrx1 in the blood and TfTrp14 in the brain. In post-LPS and heavy metal challenge, the mRNA of both genes was significantly increased in the skin, liver, spleen, and brain at various times. The results of western blot detection displayed that the time of the induced maximum protein expression was 6-h post-LPS injection in the skin and liver, which were slightly delayed compared with that of 2 h at mRNA level. The recombinant TfTrp14 and TfTrx1 proteins were expressed in E. coli BL21 (DE3). The increase of the fluorescence intensity in rTfTrx1 and rTfTrp14 suggested the redox state changes in the microenvironment around tryptophan residues. Both of the recombinant proteins exhibited concentration-dependent disulfide reductase activity towards insulin, and the catalytic activity of rTfTrx1 was much higher than that of rTfTrp14.
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http://dx.doi.org/10.1007/s10695-021-00978-xDOI Listing
July 2021

Structural insights into hormone recognition by the human glucose-dependent insulinotropic polypeptide receptor.

Elife 2021 Jul 13;10. Epub 2021 Jul 13.

School of Pharmacy, Fudan University, Shanghai, China.

Glucose-dependent insulinotropic polypeptide (GIP) is a peptide hormone that exerts crucial metabolic functions by binding and activating its cognate receptor, GIPR. As an important therapeutic target, GIPR has been subjected to intensive structural studies without success. Here, we report the cryo-EM structure of the human GIPR in complex with GIP and a G heterotrimer at a global resolution of 2.9 Å. GIP adopts a single straight helix with its N terminus dipped into the receptor transmembrane domain (TMD), while the C terminus is closely associated with the extracellular domain and extracellular loop 1. GIPR employs conserved residues in the lower half of the TMD pocket to recognize the common segments shared by GIP homologous peptides, while uses non-conserved residues in the upper half of the TMD pocket to interact with residues specific for GIP. These results provide a structural framework of hormone recognition and GIPR activation.
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http://dx.doi.org/10.7554/eLife.68719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298097PMC
July 2021

Molecular insights into ago-allosteric modulation of the human glucagon-like peptide-1 receptor.

Nat Commun 2021 06 18;12(1):3763. Epub 2021 Jun 18.

School of Pharmacy, Fudan University, Shanghai, China.

The glucagon-like peptide-1 (GLP-1) receptor is a validated drug target for metabolic disorders. Ago-allosteric modulators are capable of acting both as agonists on their own and as efficacy enhancers of orthosteric ligands. However, the molecular details of ago-allosterism remain elusive. Here, we report three cryo-electron microscopy structures of GLP-1R bound to (i) compound 2 (an ago-allosteric modulator); (ii) compound 2 and GLP-1; and (iii) compound 2 and LY3502970 (a small molecule agonist), all in complex with heterotrimeric G. The structures reveal that compound 2 is covalently bonded to C347 at the cytoplasmic end of TM6 and triggers its outward movement in cooperation with the ECD whose N terminus penetrates into the GLP-1 binding site. This allows compound 2 to execute positive allosteric modulation through enhancement of both agonist binding and G protein coupling. Our findings offer insights into the structural basis of ago-allosterism at GLP-1R and may aid the design of better therapeutics.
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http://dx.doi.org/10.1038/s41467-021-24058-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213797PMC
June 2021

HDAC6 regulates primordial follicle activation through mTOR signaling pathway.

Cell Death Dis 2021 May 29;12(6):559. Epub 2021 May 29.

State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, 100193, Beijing, China.

Primordial follicle pool established perinatally is a non-renewable resource which determines the female fecundity in mammals. While the majority of primordial follicles in the primordial follicle pool maintain dormant state, only a few of them are activated into growing follicles in adults in each cycle. Excessive activation of the primordial follicles accelerates follicle pool consumption and leads to premature ovarian failure. Although previous studies including ours have emphasized the importance of keeping the balance between primordial follicle activation and dormancy via molecules within the primordial follicles, such as TGF-β, E-Cadherin, mTOR, and AKT through different mechanisms, the homeostasis regulatory mechanisms of primordial follicle activation remain unclear. Here, we reported that HDAC6 acts as a key negative regulator of mTOR in dormant primordial follicles. In the cytoplasm of both oocytes and granulosa cells of primordial follicles, HDAC6 expressed strong, however in those activated primordial follicles, its expression level is relatively weaker. Inhibition or knockdown of HDAC6 significantly promoted the activation of limited primordial follicles while the size of follicle pool was not affected profoundly in vitro. Importantly, the expression level of mTOR in the follicle and the activity of PI3K in the oocyte of the follicle were simultaneously up-regulated after inhibiting of HDAC6. The up-regulated mTOR leads to not only the growth and differentiation of primordial follicles granulosa cells (pfGCs) into granulosa cells (GCs), but the increased secretion of KITL in these somatic cells. As a result, inhibition of HDAC6 awaked the dormant primordial follicles of mice in vitro. In conclusion, HDAC6 may play an indispensable role in balancing the maintenance and activation of primordial follicles through mTOR signaling in mice. These findings shed new lights on uncovering the epigenetic factors involved physiology of sustaining female reproduction.
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http://dx.doi.org/10.1038/s41419-021-03842-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164630PMC
May 2021

Characteristics of TCM Constitution and Related Biomarkers for Mild Cognitive Impairment.

Neuropsychiatr Dis Treat 2021 20;17:1115-1124. Epub 2021 Apr 20.

Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning, 530023, People's Republic of China.

Introduction: The incidence of Alzheimer's disease is on the rise, early detection of cognitive impairment of the elderly is very important. In traditional Chinese medicine, constitution is related to the susceptibility of the human body to diseases. Based on the theory of constitution of traditional Chinese medicine (TCM), the human population can be classified into 9 constitutions. However, little is known about the characteristics of medical constitution and related biomarkers in subjects with mild cognitive impairment (MCI).

Methods: We measured the TCM Constitution of 214 subjects by using the Constitution in Chinese Medicine Questionnaire (CCMQ). MMSE and MoCA were used to assess cognitive function. The subjects were divided into mild cognitive impairment group (MCI, n = 152) and normal control group (NC, n = 62). The levels of serum Hcy and serum/urine 8-iso-PGF 2α were determined.

Results: 1) It was found that there was a significant difference in constitution types between MCI and NC. There were significant differences in MMSE and MoCA score, serum Hcy and serum/urine 8-iso-PGF 2a levels between the two groups. 2) In logistic regression analysis, the variables with statistical significance were TCM Constitution of Yang-Deficient, Phlegm-Dampness, Blood-Stasis and abnormal increase of Hcy (OR>1). 3) The MoCA scores had a positive correlation with the MMSE. A statistically significant inverse association was found between serum Hcy, blood and urine 8-iso-PGF 2a and scores of cognitive assessment in MCI.

Conclusion: Constitution types (Yang-Deficient, Phlegm-Dampness and Blood-Stasis) and abnormal serum Hcy elevation can be used as risk factors for MCI. MoCA scores can serve to detect MCI at early stage. Serum/urine 8-iso-PGF 2α has a certain relationship with MCI. Higher levels of serum/urine 8-iso-PGF 2α are more likely to be associated with MCI risk.
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http://dx.doi.org/10.2147/NDT.S290692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068505PMC
April 2021

A Pilot Study on the Cutoff Value of Related Brain Metabolite in Chinese Elderly Patients With Mild Cognitive Impairment Using MRS.

Front Aging Neurosci 2021 9;13:617611. Epub 2021 Apr 9.

Department of Rehabilitation, Bao'an Hospital, Southern Medical University, Shenzhen, China.

This cross-sectional study aimed to distinguish patients with mild cognitive impairment (MCI) from patients with normal controls (NCs) by measuring the levels of -acetyl aspartate (NAA), total creatinine (tCr), and choline (Cho) in their hippocampus (HIP) and their posterior cingulate gyrus (PCG) by using proton magnetic resonance spectroscopy (MRS) and to predict the cutoff value on the ratios of metabolites. We further aimed to provide a reference for the diagnosis of MCI in elderly patients in China. About 69 patients who underwent a clinical diagnosis of the MCI group and 67 patients with NCs, the Mini-Mental Status Examination (MMSE) score, the Montreal Cognitive Assessment (MoCA) score, and MRS of the bilateral HIP and bilateral PCG were considered. The ratio of NAA/tCr and Cho/tCr in the bilateral HIP and bilateral PCG was calculated. The relationship between the ratios of metabolites and the scores of MMSE and MoCA was analyzed, and the possible brain metabolite cutoff point for the diagnosis of MCI was evaluated. Compared with the NC group, the scores of MMSE and MoCA in the MCI group decreased significantly ( < 0.05); the ratio of NAA/tCr in the bilateral HIP and bilateral PCG and the ratio of Cho/tCr at the right HIP in the MCI group decreased significantly ( < 0.05); however, there was no significant difference in the ratio of Cho/tCr in the left HIP and bilateral PCG between the two groups ( > 0.05). The correlation coefficient between MMSE/MoCA and the ratio of NAA/tCr was 0.49-0.56 in the bilateral HIP ( < 0.01). The best cutoff value of NAA/creatine (Cr) in the left HIP and the right HIP was 1.195 and 1.19. Sensitivity, specificity, and the Youden index (YDI) in the left HIP and the right HIP were (0.725, 0.803, 0.528) and (0.754, 0.803, 0.557), respectively. The level of metabolites in the HIP and the PCG of patients with MCI and of those with normal subjects has a certain correlation with the score of their MMSE and MoCA. When the value of NAA/tCr in the left HIP and right HIP is <1.19, it suggests that MCI may have occurred. According to this cutoff point, elderly patients with MCI in China could be screened.
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http://dx.doi.org/10.3389/fnagi.2021.617611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063036PMC
April 2021

CRISPR/Cas9-Mediated α-ENaC Knockout in a Murine Pancreatic β-Cell Line.

Front Genet 2021 1;12:664799. Epub 2021 Apr 1.

Department of Pathophysiology, Nanjing Medical University, Nanjing, China.

Many ion channels participate in controlling insulin synthesis and secretion of pancreatic β-cells. Epithelial sodium channel (ENaC) expressed in human pancreatic tissue, but the biological role of ENaC in pancreatic β-cells is still unclear. Here, we applied the CRISPR/Cas9 gene editing technique to knockout α-ENaC gene in a murine pancreatic β-cell line (MIN6 cell). Four single-guide RNA (sgRNA) sites were designed for the exons of α-ENaC. The sgRNA1 and sgRNA3 with the higher activity were constructed and co-transfected into MIN6 cells. Through processing a series of experiment flow included drug screening, cloning, and sequencing, the α-ENaC gene-knockout (α-ENaC) in MIN6 cells were obtained. Compared with the wild-type MIN6 cells, the cell viability and insulin content were significantly increased in α-ENaC MIN6 cells. Therefore, α-ENaC MIN6 cells generated by CRISPR/Cas9 technology added an effective tool to study the biological function of α-ENaC in pancreatic β-cells.
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http://dx.doi.org/10.3389/fgene.2021.664799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047203PMC
April 2021

Trace Identification and Visualization of Multiple Benzimidazole Pesticide Residues on Leaves Using Terahertz Imaging Combined with Deep Learning.

Int J Mol Sci 2021 Mar 26;22(7). Epub 2021 Mar 26.

College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

Molecular spectroscopy has been widely used to identify pesticides. The main limitation of this approach is the difficulty of identifying pesticides with similar molecular structures. When these pesticide residues are in trace and mixed states in plants, it poses great challenges for practical identification. This study proposed a state-of-the-art method for the rapid identification of trace (10 mg·L) and multiple similar benzimidazole pesticide residues on the surface of leaves, mainly including benzoyl (BNL), carbendazim (BCM), thiabendazole (TBZ), and their mixtures. The new method combines high-throughput terahertz (THz) imaging technology with a deep learning framework. To further improve the model reliability beyond the THz fingerprint peaks (BNL: 0.70, 1.07, 2.20 THz; BCM: 1.16, 1.35, 2.32 THz; TBZ: 0.92, 1.24, 1.66, 1.95, 2.58 THz), we extracted the absorption spectra in frequencies of 0.2-2.2 THz from images as the input to the deep convolution neural network (DCNN). Compared with fuzzy Sammon clustering and four back-propagation neural network (BPNN) models (TrainCGB, TrainCGF, TrainCGP, and TrainRP), DCNN achieved the highest prediction accuracies of 100%, 94.51%, 96.26%, 94.64%, 98.81%, 94.90%, 96.17%, and 96.99% for the control check group, BNL, BCM, TBZ, BNL + BCM, BNL + TBZ, BCM + TBZ, and BNL + BCM + TBZ, respectively. Taking advantage of THz imaging and DCNN, the image visualization of pesticide distribution and residue types on leaves was realized simultaneously. The results demonstrated that THz imaging and deep learning can be potentially adopted for rapid-sensing detection of trace multi-residues on leaf surfaces, which is of great significance for agriculture and food safety.
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http://dx.doi.org/10.3390/ijms22073425DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8037687PMC
March 2021

Influence of incentive nursing intervention on recovery of burn patients after vacuum sealing drainage.

Int Wound J 2021 Mar 18. Epub 2021 Mar 18.

Department of Burn and Plastic Surgery, The First Hospital of Hebei Medical University, Shijiazhuang, China.

To observe the effect of application of incentive nursing intervention (INI) on recovery in burn patients undergoing vacuum sealing drainage (VSD). From January 2017 to January 2020, a total of 82 consecutive burn patients were prospectively enrolled, and divided into INI group and routine nursing intervention (RNI) group according to random number table method. The causes of inadequate drainage were collected, the incidence was calculated, and the occurrence of inadequate drainage at different locations was compared. The pain degree and comfort status before and after the intervention were observed, and the wound healing time, hospital stay, and satisfaction after the intervention were recorded. The reasons for inadequate drainage during the treatment of VSD included negative pressure insufficient, drainage tube blockage because of escharosis, replacement of negative pressure internal sac not standard, loose sealing of the semi-permeable membrane, and the negative pressure tube fell off, compressed or reflexed. The baseline characteristics between the two groups were comparable (P > .05). The incidence of each cause and total incidence of inadequate drainage in INI group were lower than those in RNI group (P < .05, respectively). The incidences of inadequate drainage of all burn sites in INI group were lower than those in RNI group, and the difference of limbs wound between the two group was statistically significant (P < .05). After intervention, the pain intensity of INI group was lower than that of RNI group (P < .05), and the holistic comfort of INI group was higher than that of RNI group (P < .05). The wound healing time and hospital stay time in INI group were lower than those in RNI group, and the total satisfaction rate in INI group was higher than that in RNI group (P < .05, respectively). Applying INI can effectively reduce the incidence of insufficient drainage, reduce pain, improve comfort, shorten wound healing time and hospital stay, and thus improve the overall satisfaction rate of patients, which is worthy of clinical promotion and application.
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http://dx.doi.org/10.1111/iwj.13582DOI Listing
March 2021

Evaluation of correlation between sagittal balance and plantar pressure distributions in adolescent idiopathic scoliosis: A pilot study.

Clin Biomech (Bristol, Avon) 2021 Mar 26;83:105308. Epub 2021 Feb 26.

Department of Orthopedics, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, PR China. Electronic address:

Background: To evaluate the correlation between baropodometric parameters and sagittal parameters for adolescent idiopathic scoliosis.

Methods: 44 volunteers (7 males and 37 females) were recruited. All participants were diagnosed as adolescent idiopathic scoliosis by X-ray before baropodometric study. Sagittal parameters included thoracic kyphosis, lumbar lordosis, sagittal vertical axis, pelvic tilt, sacral slope and pelvic incidence. A static baropodometry was performed for each patient. The foot area was divided into four quadrants. The contact surface and weight percentage were measured.

Findings: Lumbar lordosis was positively correlated to pelvic incidence, sacral slope and (P < 0.001, P < 0.001, respectively). On the major curve side, pelvic tilt showed a positive correlation with all baropodometric parameters (P < 0.05) except forefoot weight percentage. Thoracic kyphosis showed negative correlations with contact surface and weight percentage of the forefoot (P = 0.04, 0.02 respectively) but no correlation with any rearfoot feature. Lumbar lordosis, sagittal vertical axis, pelvic incidence and sacral slope were not in correlation with plantar pressure. On the opposite side, sagittal profile showed no obvious correlation with any baropodometric parameter.

Interpretation: In scoliosis, sagittal balance is closely correlated to plantar pressure distributions. Baropodometry is a feasible method to assess sagittal balance.
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http://dx.doi.org/10.1016/j.clinbiomech.2021.105308DOI Listing
March 2021

Effects of energy supplements on the differentiation of skeletal muscle satellite cells.

Food Sci Nutr 2021 Jan 13;9(1):357-366. Epub 2020 Dec 13.

College of Food Science and Engineering Inner Mongolia Agriculture University Hohhot China.

To investigate the effects of the activator of AMPK and high glucose on the differentiation of mouse SMSCs, primary SMSCs were isolated from mouse extensor digitorum longus muscle and grown to near confluence (80%). Postconfluent cells were cultured in a growth medium with different inductors: AICAR, glucose, and AICAR mixed with glucose. The specific protein expressions of SMSCs, myoblasts, adipocytes, and brown adipocytes were analyzed on days 0, 3, 5, 7, and 10. The results showed treatment with AICAR in SMSCs markedly activated AMPK phosphorylation ( < .05) and increased protein expression of Pax7 and MyoD ( < .05), high concentrations of intracellular glucose upregulated UCP-1 protein expression and enhanced lipid accumulation ( < .05), the cowork of AICAR and glucose affected a decrease on MyoD, PPARg, and UCP-1 expression ( < .05) and an increase on Pax7. The present study indicated that the certain energy supplements influence the direction of SMSC differentiation which may contribution on the structure of muscle and meat quality, sequentially.
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http://dx.doi.org/10.1002/fsn3.2001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802567PMC
January 2021

Recurrent and concurrent patterns of regional BOLD dynamics and functional connectivity dynamics in cognitive decline.

Alzheimers Res Ther 2021 01 16;13(1):28. Epub 2021 Jan 16.

The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, Guangxi, China.

Background: The brain's dynamic spontaneous neural activity and dynamic functional connectivity (dFC) are both important in supporting cognition, but how these two types of brain dynamics evolve and co-evolve in subjective cognitive decline (SCD) and mild cognitive impairment (MCI) remain unclear. The aim of the present study was to investigate recurrent and concurrent patterns of two types of dynamic brain states correlated with cognitive decline.

Methods: The present study analyzed resting-state functional magnetic resonance imaging data from 62 SCD patients, 75 MCI patients, and 70 healthy controls (HCs). We used the sliding-window and clustering method to identify two types of recurrent brain states from both dFC and dynamic regional spontaneous activity, as measured by dynamic fractional amplitude of low-frequency fluctuations (dfALFF). Then, the occurrence frequency of a dFC or dfALFF state and the co-occurrence frequency of a pair of dFC and dfALFF states among all time points are extracted for each participant to describe their dynamics brain patterns.

Results: We identified a few recurrent states of dfALFF and dFC and further ascertained the co-occurrent patterns of these two types of dynamic brain states (i.e., dfALFF and dFC states). Importantly, the occurrence frequency of a default-mode network (DMN)-dominated dFC state was significantly different between HCs and SCD patients, and the co-occurrence frequencies of a DMN-dominated dFC state and a DMN-dominated dfALFF state were also significantly different between SCD and MCI patients. These two dynamic features were both significantly positively correlated with Mini-Mental State Examination scores.

Conclusion: Our findings revealed novel fMRI-based neural signatures of cognitive decline from recurrent and concurrent patterns of dfALFF and dFC, providing strong evidence supporting SCD as the transition phase between normal aging and MCI. This finding holds potential to differentiate SCD patients from HCs via both dFC and dfALFF as objective neuroimaging biomarkers, which may aid in the early diagnosis and intervention of Alzheimer's disease.
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http://dx.doi.org/10.1186/s13195-020-00764-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7811744PMC
January 2021

Quinolizidine Alkaloids with Antiviral and Insecticidal Activities from the Seeds of Gagnep.

J Agric Food Chem 2020 Dec 7;68(50):15015-15026. Epub 2020 Dec 7.

State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, PR China.

The discovery of novel, effective, and botanical pesticides is one of the main strategies for modern plant protection and insect pest control. During the search for novel botanical pesticides from natural sources, the seeds of were systematically investigated to obtain 11 new matrine-type alkaloids (-), including one novel matrine-type alkaloid featuring an unprecedented 5/6/6/6 tetracyclic skeleton (), along with 16 known compounds (-). Their structures were elucidated by comprehensive spectroscopic data analysis (IR, UV, NMR, and HRESIMS), ECD calculations, and single-crystal X-ray diffraction. The anti-tobacco mosaic virus (TMV) activity and insecticidal activities against and of the compounds were also respectively screened using the half-leaf method and spray method. Biological tests indicated that compounds , , , and displayed significant anti-TMV biological activities compared with the positive control ningnanmycin. Compounds , , and presented moderate activities against with LC values of 38.29, 18.63, and 23.74 mg/L, respectively. Moreover, compounds and exhibited weak activities against .
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http://dx.doi.org/10.1021/acs.jafc.0c06032DOI Listing
December 2020

Effects of supplementation on oxidative stability of Cashmere goat.

Food Sci Nutr 2020 Oct 1;8(10):5550-5556. Epub 2020 Sep 1.

College of Food Science and Engineering Inner Mongolia Agricultural University Hohhot China.

(AM) provides a rich source of polysaccharides that can act as powerful antioxidants, but their potential as feed ingredients in the lamb industry still rarely exploited. The objective of this study was to investigate the effect of dietary supplementation on oxidative stability of goat muscles. Longissimus dorsi (LD) muscles from two groups of Cashmere goat (basal diet, C group; basal diet supplemented with 1% root, AM group) were evaluated for lipid oxidation, myoglobin oxidation, activity of antioxidant enzymes, and antioxidant capacity. The results showed that color parameters in Cashmere goat of two feeding conditions were no significant difference ( > .05). In AM group, myoglobin (Mb) content was higher than C, while metmyoglobin (MMb) ( < .05) and malondialdehyde (MDA) ( < .01) were lower. Additionally supplementation had a significant effect on superoxide dismutase (SOD) and catalase (CAT) ( < .001). In whole, the AM group goats presented a relatively higher antioxidant capacity than C. Especially, RSA and CUPRAC values of AM group goats had significantly higher than C ( < .05). Consequently, the AM group goats ingested abundant which enhanced the antioxidant capacity. Thus, it can eliminate free radicals and effectively inhibit oxidation.
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http://dx.doi.org/10.1002/fsn3.1786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590321PMC
October 2020

Ultrasound assisted dispersive solid phase microextraction of inorganic arsenic from food and water samples using CdS nanoflowers combined with ICP-OES determination.

Food Chem 2021 Feb 8;338:128028. Epub 2020 Sep 8.

College of Chemistry and Environmental Engineering, Shenzhen University, 1066 Xueyuan Ave., Shenzhen 518055, PR China.

Direct determination of arsenic species in real samples is challenging due to their trace concentration and spectral interferences by coexisting ions. Herein, we proposed an ultrasound-assisted dispersive solid phase microextraction (DSPME) procedure for the analyses of the trace inorganic arsenic. The hydrothermally synthesized cadmium sulfide nanoparticles (CdS NPs) completely adsorbed both arsenic species within 20 s at the initial arsenic concentration of 100 µg L. The detection limit (3 S/m) of the proposed method was found to be 0.5 ± 0.2 and 0.8 ± 0.2 ng L for As(III) and As(V), respectively. The accuracy of the method against the systematic and constant errors was confirmed by the analysis of the Standard Reference Material (SRM) (>95% recovery with <5% RSD). The Student's t-test values were found to be less than the critical Student's t value at a 95% confidence level. The method was successfully employed for the determination of arsenic in food samples.
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http://dx.doi.org/10.1016/j.foodchem.2020.128028DOI Listing
February 2021

Genomes of the Banyan Tree and Pollinator Wasp Provide Insights into Fig-Wasp Coevolution.

Cell 2020 11 8;183(4):875-889.e17. Epub 2020 Oct 8.

Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. Electronic address:

Banyan trees are distinguished by their extraordinary aerial roots. The Ficus genus includes species that have evolved a species-specific mutualism system with wasp pollinators. We sequenced genomes of the Chinese banyan tree, F. microcarpa, and a species lacking aerial roots, F. hispida, and one wasp genome coevolving with F. microcarpa, Eupristina verticillata. Comparative analysis of the two Ficus genomes revealed dynamic karyotype variation associated with adaptive evolution. Copy number expansion of auxin-related genes from duplications and elevated auxin production are associated with aerial root development in F. microcarpa. A male-specific AGAMOUS paralog, FhAG2, was identified as a candidate gene for sex determination in F. hispida. Population genomic analyses of Ficus species revealed genomic signatures of morphological and physiological coadaptation with their pollinators involving terpenoid- and benzenoid-derived compounds. These three genomes offer insights into and genomic resources for investigating the geneses of aerial roots, monoecy and dioecy, and codiversification in a symbiotic system.
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http://dx.doi.org/10.1016/j.cell.2020.09.043DOI Listing
November 2020

Analysis of Cyclooxygenase 2, Programmed Cell Death Ligand 1, and Arginase 1 Expression in Human Pituitary Adenoma.

World Neurosurg 2020 12 10;144:e660-e673. Epub 2020 Sep 10.

Department of Neurosurgery, Affiliated Hospital of Jining Medical University, Jining, Shandong Province, China. Electronic address:

Background: Cyclooxygenase 2 (COX-2) is a key enzyme in the synthesis of prostaglandins. Recent studies have shown that overexpression of COX-2 can reduce the antitumor effect of the immune system by inhibiting the proliferation of B and T lymphocytes. Programmed cell death ligand 1 (PD-L1) was the first functionally characterized ligand of programmed cell death protein 1. It plays an important role in maintaining peripheral and central immune tolerance by combining with programmed cell death protein 1. Arginase 1 (ARG1) can process L-arginine in the local microenvironment and affect the function of T cells, resulting in immune escape. In this study, COX-2, PD-L1, and ARG1 expression in human pituitary adenoma (PA) and their relationship were investigated, which provided an initial theoretic basis for further study of the immune escape mechanism in PA in cellular and animal experiments.

Methods: The protein expression of COX-2, PD-L1, and ARG1 in 55 PA samples was detected by immunohistochemistry, with 10 normal brain tissues as the control group. The location of COX-2, PD-L1, and ARG1 in PA cells was studied by double immunofluorescence colocalization. The results of immunohistochemistry were further verified by Western blot.

Results: The expression of COX-2, PD-L1, and ARG1 in PA was significantly higher than that in normal brain tissue. In functional PA (FPA) and nonfunctional PA (NFPA), there was no significant difference in the expression of COX-2 and PD-L1, whereas ARG1 was higher in NFPA. Moreover, the protein expression level of COX-2 was positively correlated with that of PD-L1 and ARG1, and the expression of PD-L1 was positively correlated with that of ARG1. Immunofluorescence confocal imaging showed that COX-2, PD-L1, and ARG1 were all expressed in the cytoplasm of PA cells, and the physical positions of COX-2, PD-L1, and ARG1 were partially coincident.

Conclusions: These findings indicate that overexpression of COX-2, PD-L1, and ARG1 may be involved in the pathogenesis of PA. ARG1 plays a more important role in the development of NFPA. By upregulating the expression of PD-L1, COX-2 may promote the expression of ARG1, forming the COX-2/PD-L1/ARG1 signal pathway in promoting the occurrence and development of PA. Perhaps further study of the pathogenesis of PA can start with the mechanism of immune escape.
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http://dx.doi.org/10.1016/j.wneu.2020.09.031DOI Listing
December 2020

Floral transcriptomes reveal gene networks in pineapple floral growth and fruit development.

Commun Biol 2020 09 10;3(1):500. Epub 2020 Sep 10.

Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Life Sciences, College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Proper flower development is essential for sexual reproductive success and the setting of fruits and seeds. The availability of a high quality genome sequence for pineapple makes it an excellent model for studying fruit and floral organ development. In this study, we sequenced 27 different pineapple floral samples and integrated nine published RNA-seq datasets to generate tissue- and stage-specific transcriptomic profiles. Pairwise comparisons and weighted gene co-expression network analysis successfully identified ovule-, stamen-, petal- and fruit-specific modules as well as hub genes involved in ovule, fruit and petal development. In situ hybridization confirmed the enriched expression of six genes in developing ovules and stamens. Mutant characterization and complementation analysis revealed the important role of the subtilase gene AcSBT1.8 in petal development. This work provides an important genomic resource for functional analysis of pineapple floral organ growth and fruit development and sheds light on molecular networks underlying pineapple reproductive organ growth.
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http://dx.doi.org/10.1038/s42003-020-01235-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7483743PMC
September 2020

Prevalence and Clinical Significance of Occult Fractures in the Extremities in Children.

Front Pediatr 2020 4;8:393. Epub 2020 Aug 4.

Department of Orthopedics, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai, China.

Diagnosis of occult fractures by initial plain radiographs remains challenging in children in the emergency room. This study was to assess the prevalence and distribution of occult fracture in children with acute extremities injuries (AEI) and clinical suspicion of fracture. We conducted a retrospective study to review the medical records of all pediatric patients with AEI in the orthopedic emergency room from January 1, 2017, to December 31, 2019. For patients with concerning history and physical examination but negative initial radiographs, we conducted the following three diagnostic strategies according to the choic of children's parents: immediate MRI scanning, [2] immediate CT scanning, or [3] empiric cast immobilization with orthopedic follow-up radiographs at 2 weeks post-injury (late radiographs). Prevalence and distribution of occult fracture were recorded. A total of 43,560 pediatric patients meet the inclusion criteria. A total of 4,916 fractures of the extremities were confirmed by initial plain radiographs, and 550 occult fractures were confirmed by immediate MRI, immediate CT, or late radiographs. The prevalence of occult fracture in the extremities was 10.1% (550/5,466). Supracondylar fractures were the most prevalent (2,325/5,466, 42.5%) but had the lowest rate of occult fractures (117/2,325, 5.0%). The highest rate of occult fracture was distal epiphyseal fracture of the tibia and fibula (49/145, 33.8%), but these had a relatively lower prevalence of fractures (145/5,466, 2.65%). We should be aware of the relative high prevalence of occult fractures in the extremities in children, especially when the injured site is in the high incidence area of occult fracture such as ankle.
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http://dx.doi.org/10.3389/fped.2020.00393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7438767PMC
August 2020

The effect of different starter cultures on biogenic amines and quality of fermented mutton sausages stored at 4 and 20°C temperatures.

Food Sci Nutr 2020 Aug 8;8(8):4472-4483. Epub 2020 Jul 8.

College of Food Science and Engineering Inner Mongolia Agricultural University Hohhot People's Republic of China.

The biogenic amines (BAs), water activity, pH, thiobarbituric acid-reactive substances (TBARS), and nitrite were, respectively, tested in dry fermented sausage with starter cultures (37x-6 , x3-3b , 30x-11 , and 37x-8 . ), during storage of room temperature (20°C) and refrigeration storage (4°C). Tryptamine (TRM), 2-phenylethylamine (PHE), putrescine (PUT), cadaverine (CAD), histamine (HIM), and tyramine (TYM) contents of all samples were increased storage at 20°C, and the content of TRM, PUT, CAD, and HIM of all samples storage at 20°C was higher than that storage at 4°C after 42 days. The content of BA with 37x-6, x3-3b, and 37x-8 was obviously decreased at 4°C storage. The storage temperature has a significant effect on BA content ( < .05) for TYM and other BAs tested. Finally, x3-3b, 37x-6, and 37x-8 should be used to produce fermented sausages on the basis of the concentration of BAs.
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http://dx.doi.org/10.1002/fsn3.1748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7455928PMC
August 2020

Tagitinin A from Tithonia diversifolia provides resistance to tomato spotted wilt orthotospovirus by inducing systemic resistance.

Pestic Biochem Physiol 2020 Oct 15;169:104654. Epub 2020 Jul 15.

Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Yunnan Provincial Key Laboratory of Agricultural Biotechnology, Key Lab of Southwestern Crop Gene Resource and Germplasm Innovation, Ministry of Agriculture, Kunming 650204, Yunnan, China. Electronic address:

Tomato spotted wilt orthotospovirus (TSWV) causes devastating losses to agronomic and ornamental crops worldwide. Currently, there is no effective strategy to control this disease. Use of biotic inducers to enhance plant resistance to viruses maybe an effective approach. Our previous study indicated that Tagitinin A (Tag A) has a high curative and protective effect against TSWV. However, the underlying molecular mechanism of Tag A-mediated antiviral activity remains unknown. In this study, Tag A reduced the expression of the NSs, NSm genes was very low in untreated leaves following TSWV infection. In addition, the expression of all TSWV genes in the inoculated and systemic leaves was inhibited in the protective assay, and with an inhibition rate of more than 85% in systemic leaves. Tag A increased phenylalanine ammonia-lyase (PAL) activity in the curative and protective assays. The concentrations of jasmonic acid (JA) and jasmonic acid -isoleucine (JA-Ile) and the expression of its key gene NtCOI1 in Tag A-treated and systemic leaves of treated plants were significantly higher than those of the control plant. Furthermore, Tag A-induced resistance to TSWV could be eliminated by VIGS-mediated silencing of the NtCOI1 gene. These indicated that Tag A acts against TSWV by activating the JA defense signaling pathway.
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http://dx.doi.org/10.1016/j.pestbp.2020.104654DOI Listing
October 2020

Structural insights into the activation of GLP-1R by a small molecule agonist.

Cell Res 2020 12 28;30(12):1140-1142. Epub 2020 Jul 28.

The CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.

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http://dx.doi.org/10.1038/s41422-020-0384-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784854PMC
December 2020

The effect of trees on human energy fluxes in a humid subtropical climate region.

Int J Biometeorol 2020 Oct 8;64(10):1675-1686. Epub 2020 Jul 8.

South China University of Technology, Guangzhou, China.

Trees are considered to be an effective tool for improving human thermal comfort in hot climates and have been widely used in landscape architecture. However, it is not always clear how trees affect human-environment energy fluxes. In this study, an in-depth analysis of four common tree species was undertaken based on comprehensive field measurements, in terms of how each tree and its characteristics affected the energy fluxes of a person in a humid subtropical climate region. Results showed that the largest effect of trees was on radiation fluxes, with a much smaller effect on the convective and evaporative fluxes. For a person standing in shade, a tree can reduce approximately 25% of the absorbed radiation compared with an open reference point. Moreover, the cooling effect on radiation components was found to be greater in the solar radiation domain than in the terrestrial radiation domain. Solar radiation and ground surface temperature had the largest effect on a human energy budget, which was affected by characteristics of the trees and the thermophysical properties of ground surfaces. The effect from relative humidity and wind speed was quite minimal. For the four common tree species in this study, Ficus microcarpa had the best thermal performance by reducing the most absorbed solar radiation flux. This study shows a detailed empirical research about the thermal effects of trees on a person, providing recommendations for tree species selection in urban design.
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http://dx.doi.org/10.1007/s00484-020-01948-3DOI Listing
October 2020

SARS-CoV-2 infection of the liver directly contributes to hepatic impairment in patients with COVID-19.

J Hepatol 2020 10 11;73(4):807-816. Epub 2020 May 11.

Department of Pathology and Hepatology, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China. Electronic address:

Background & Aims: Liver enzyme abnormalities are common in patients with coronavirus disease 2019 (COVID-19). Whether or not severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can lead to liver damage remains unknown. Herein, we reported the clinical characteristics and liver pathological manifestations of COVID-19 patients with liver enzyme abnormalities.

Methods: We analyzed 156 patients diagnosed with COVID-19 from 2 designated centers in China and compared clinical features between patients with or without elevated aminotransferases. Postmortem liver biopsies were obtained from 2 cases who had elevated aminotransferases. We investigated the patterns of liver impairment by electron microscopy, immunohistochemistry, TUNEL assay and pathological studies.

Results: Sixty-four out of 156 (41.0%) patients with COVID-19 had elevated aminotransferases. The median levels of alanine aminotransferase were 50 U/L 19 U/L, respectively, aspartate aminotransferase were 45.5 U/L 24 U/L, respectively in abnormal and normal aminotransferase groups. Liver enzyme abnormalities were associated with disease severity, as well as a series of laboratory tests including higher alveolar-arterial oxygen partial pressure difference, higher gamma-glutamyltransferase, lower albumin, decreased CD4+ T cells and B lymphocytes. Ultrastructural examination identified typical coronavirus particles, characterized by spike structures, in the cytoplasm of hepatocytes in 2 COVID-19 cases. SARS-CoV-2-infected hepatocytes displayed conspicuous mitochondrial swelling, endoplasmic reticulum dilatation and glycogen granule decrease. Histologically, massive hepatic apoptosis and some binuclear hepatocytes were observed. Taken together, both ultrastructural and histological evidence indicated a typical lesion of viral infection. Immunohistochemical results showed scarce CD4+ and CD8+ lymphocytes. No obvious eosinophil infiltration, cholestasis, fibrin deposition, granuloma, massive central necrosis, or interface hepatitis were observed.

Conclusions: SARS-CoV-2 infection in the liver directly contributes to hepatic impairment in patients with COVID-19. Hence, a surveillance of viral clearance in liver and long-term outcome of COVID-19 is required.

Lay Summary: Liver enzyme abnormalities are common in patients with coronavirus disease 2019 (COVID-19). We reported the clinical characteristics and liver pathological manifestations of COVID-19 patients with elevated liver enzymes. Our findings suggested that SARS-CoV-2 infection of the liver is a crucial factor contributing to hepatic impairment in patients with COVID-19.
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http://dx.doi.org/10.1016/j.jhep.2020.05.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211738PMC
October 2020

Clinical and pathological investigation of patients with severe COVID-19.

JCI Insight 2020 06 18;5(12). Epub 2020 Jun 18.

Department of Pathology and Hepatology.

Background: Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory coronavirus 2 (SARS-CoV-2), has become a pandemic. This study addresses the clinical and immunopathological characteristics of severe COVID-19.

Methods: Sixty-nine patients with COVID-19 were classified into severe and nonsevere groups to analyze their clinical and laboratory characteristics. A panel of blood cytokines was quantified over time. Biopsy specimens from 2 deceased cases were obtained for immunopathological, ultrastructural, and in situ hybridization examinations.

Results: Circulating cytokines, including IL-8, IL-6, TNF-α, IP10, MCP1, and RANTES, were significantly elevated in patients with severe COVID-19. Dynamic IL-6 and IL-8 were associated with disease progression. SARS-CoV-2 was demonstrated to infect type II and type I pneumocytes and endothelial cells, leading to severe lung damage through cell pyroptosis and apoptosis. In severe cases, lymphopenia, neutrophilia, depletion of CD4+ and CD8+ T lymphocytes, and massive macrophage and neutrophil infiltrates were observed in both blood and lung tissues.

Conclusions: A panel of circulating cytokines could be used to predict disease deterioration and inform clinical interventions. Severe pulmonary damage was predominantly attributed to both cytopathy caused by SARS-CoV-2 and immunopathologic damage. Strategies that prohibit pulmonary recruitment and overactivation of inflammatory cells by suppressing cytokine storm might improve the outcomes of patients with severe COVID-19.
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http://dx.doi.org/10.1172/jci.insight.138070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406259PMC
June 2020

Conversion between porcine naïve-like and primed ESCs and specific pluripotency marker identification.

In Vitro Cell Dev Biol Anim 2020 May 19;56(5):412-423. Epub 2020 May 19.

Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, Nanjing, Jiangsu, China.

Researchers currently lack standardized porcine-specific markers that would aid in distinguishing the naïve and primed states of porcine embryonic stem cells (ESCs). Here, we converted naïve-like porcine ESCs (nESCs, established in our lab) into primed-state cells, and we proposed a set of molecular criteria for evaluating the naïve porcine ESCs by comparing the two cell states. The reverse-primed porcine ESCs (rpESCs) are phenotypically stable and karyotypically intact. Alkaline phosphatase positivity and the ability to form embryonic bodies suggest that rpESCs still retain the capacity for self-renewal. Lineage-associated genes, such as Cdx2, Sox17, Eomes, Foxa, Fgf5, and Pitx2, exhibited significant expression in rpESCs. Nonetheless, LIF/3i-grown porcine ESCs treated with the small molecular weight inhibitors CHIR99021, PD0325901, and SB431542 expressed the greatest number of pluripotency marker genes, including Oct4, Sox2, Nog, Dppa5, Nr0b1, and Klf4, and at higher levels than were observed in rpESCs. Despite their general trend toward higher expression of critical pluripotency factors, the nESCs showed downregulation of Tbx3, Nanog, and c-Myc, which are considered typical naïve factors in other species. Entry of the nESCs into the developmentally primed state was also associated with a marked reduction in Lin28 expression. These findings extend the knowledge of porcine pluripotency markers and provide a backdrop for future analysis of naïve porcine pluripotency.
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http://dx.doi.org/10.1007/s11626-020-00448-3DOI Listing
May 2020

Effect of fermentation and postcooking procedure on quality parameters and volatile compounds of beef jerky.

Food Sci Nutr 2020 May 8;8(5):2316-2326. Epub 2020 Apr 8.

College of Food Science and Engineering Inner Mongolia Agricultural University Huhhot China.

In this study, jerky samples were prepared from seasoned beef with and without the addition of starter culture. They were then dried or fried and analyzed to identify the quality parameters and volatile flavor compounds. Samples (starter and control) were divided into drying (inoculated-drying, I-D; noninoculated-drying, N-D) and frying (inoculated-frying, I-F; noninoculated-frying, N-F) groups. Water activity ( ), lightness (*), and redness (*) values were significantly affected ( < .001) by the postcooking procedures (drying or frying). Hardness, chewiness, and resilience were significantly lower in the dried groups than in the fried groups ( < .05). The formation of volatile compounds was affected by cooking treatment, with the highest levels observed in the I-F group. After frying, the most abundant flavor compounds in the I-D and N-D groups were terpenes and sulfur-containing compounds, followed by aromatic hydrocarbons, ketones, and alcohols. The most common compounds among all groups were acetoin, D-limonene, anethole, styrene, and tetramethylpyrazine. Overall, the jerky in the I-F group had the best color and tenderness scores among all groups.
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http://dx.doi.org/10.1002/fsn3.1515DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215205PMC
May 2020

Effect of feeding regimen on meat quality, MyHC isoforms, AMPK, and PGC-1α genes expression in the biceps femoris muscle of Mongolia sheep.

Food Sci Nutr 2020 May 14;8(5):2262-2270. Epub 2020 Apr 14.

College of Food Science and Engineering Inner Mongolia Agricultural University Hohhot China.

The effects of two feeding regimens on meat quality, myosin heavy chain (MyHC) types, and key factors regulating muscle fiber type (AMP-activated protein kinase [AMPK] and peroxisome proliferator-activated receptor-coactivator-1α [PGC-1α]) in the biceps femoris muscle of Mongolia sheep were investigated. A total of 20 Mongolia sheep were weaning for 90 days and divided into two groups (pasture group (P) and confinement group (C)) at 10.36 ± 0.35 kg of weaning weight. After weaning, sheep were pasture fed or confinement fed for 9 months. The results showed that live weights, carcass weight, intramuscular fat (IMF), and Warner-Bratzler shear force (WBSF) in P group were significantly lower ( < .05) than that in C group. Compared with P group, color evaluations with respect to L* and b* values were significantly higher ( < .05) in C group. Expression of the MyHC I gene in the P group was significantly higher, while MyHC IIa and MyHC IIb genes expression was significantly lower ( < .05) than that in C group. Also, AMPK activity and expression of AMPKα2 and PGC-1α genes were significantly higher ( < .05) in P group compared with C group. The present study indicated that muscle fiber composition was one of the key differences leading to the differences of meat quality in different feeding regimens. AMPK, particularly AMPKα2, and PGC-1α were considered to be two key factors regulating muscle fiber types in Mongolia sheep. The results support that AMPK activity and the expression of AMPKα2 and PGC-1α genes may affect the composition of muscle fibers; thus, AMPK activity and the expression of AMPKα2 and PGC-1α genes had an effect on meat quality by changed composition of muscle fibers.
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http://dx.doi.org/10.1002/fsn3.1494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215223PMC
May 2020

Colorimetric and fluorescent dual-mode strategy for sensitive detection of sulfide: Target-induced horseradish peroxidase deactivation.

Spectrochim Acta A Mol Biomol Spectrosc 2020 Aug 23;236:118296. Epub 2020 Mar 23.

Department of Pharmacy, Anhui Provincial Corps Hospital of Chinese People's Armed Police Forces, Hefei 230061, PR China. Electronic address:

Environmental pollution caused by sulfide compounds has become a major problem for public health. Hence, accurate detection of sulfide anions (S) level is valuable and vital for environmental monitoring and protection. Here, we report a new colorimetric/fluorescent dual-mode sensor for the determination of S based on the inhibition of enzyme activity and the unique optical properties of produced 2,3-diaminophenazine (DAP), thus making the analytical results more convincing. In this strategy, horseradish peroxidase (HRP) enzyme is used for catalyzing the HO-mediated oxidation of o-phenylenediamine (OPD) to produce DAP, and the color changed to bright yellow and produced orange yellow fluorescence. But the presence of S could cause the deactivation of HRP, which decreased the amount of DAP and consequently resulted in a substantial SPR band fading and an evident fluorescence quenching simultaneously. The mechanism of S sensor was examined by combining the UV-vis absorption spectra, fluorescence spectra and electrospray ionization mass spectrometry analysis. Under optimal conditions, the colorimetric and fluorescent linear responses of the proposed method exhibited a wide linear range from 2.5 nM-7.5 μM with ultralow detection limits of 1.2 nM and 0.9 nM, respectively. Some potential interferents (such as F, Cl, Br, I, SO, SO, SCN, HPO, HPO, Ac, NO, CO) in real samples showed no interference. Moreover, the proposed method offered advantages of simple, low-cost instruments and rapid assay without the utilization of nanomaterials and has been successfully applied to determine S content in lake water samples with satisfying recoveries over 97.6%. More importantly, the present S sensor not only afforded a new optical sensing pattern for bioanalysis and environment monitoring, but also extends the application field of HRP-catalyzed OPD-HO system.
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http://dx.doi.org/10.1016/j.saa.2020.118296DOI Listing
August 2020