Publications by authors named "Lihua Cao"

65 Publications

Direct generation of relativistic isolated attosecond pulses in transmission from laser-driven plasmas.

Opt Lett 2021 Mar;46(6):1285-1288

Isolated attosecond pulses are useful to perform pump-probe experiments at a high temporal resolution, and provide a new tool for ultrafast metrology. However, it is still a challenging task to generate such pulses of high intensity, even for a few-cycle laser. Through particle-in-cell simulations, we show that it is possible to directly generate a giant isolated attosecond pulse in the transmission direction from relativistic laser-driven plasmas. Compared to attosecond pulse generation in the reflection direction, no further spectral filtering is needed. The underlying radiation mechanism is coherent synchrotron emission, and the transmitted isolated attosecond pulse can reach relativistic intensity. This provides a promising alternative to generate intense isolated attosecond pulses for ultrafast studies.
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http://dx.doi.org/10.1364/OL.418144DOI Listing
March 2021

AQP8 participates in oestrogen-mediated buffalo follicular development by regulating apoptosis of granulosa cells.

Reprod Domest Anim 2021 Feb 27. Epub 2021 Feb 27.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, China.

Aquaporins (AQPs), a family of small membrane-spanning proteins, are involved in fluid transport, cell signalling and reproduction. Regulating AQP8 expression influences apoptosis of granulosa cells (GCs), ovarian folliculogenesis, oogenesis and early embryonic development in mice, but its role has never been investigated in other species. The aim of the present study was to characterize the AQP8 function in buffalo follicular development. The expression pattern of AQP8 in buffalo follicle was analysed by immunohistochemistry method. 17β-Estradiol (E2) or oestrogen receptor antagonist ICI182780 was used to treat GCs cultured in vitro, and the expression of AQP8 was detected using qRT-PCR. Its roles in apoptosis of buffalo GCs were investigated by shRNA technology. AQP8 was found to be expressed higher in secondary follicles (p < .05), and its mRNA level in GCs was upregulated by E2 via receptor-mediated mechanism in a dose-dependent manner. A 732-bp buffalo AQP8 coding region was obtained, which was highly conserved at the amino acid level among different species. AQP8-shRNA2 had more effective inhibition on target gene than AQP8-shRNA1 (66.49% vs. 58.31%) (p < .05). Knockdown of AQP8 induced GCs arrested at G2/M stage and occurred apoptosis. Compared with the control group, higher Caspase9 expression were observed in AQP8-shRNA2 lentivirus infected GCs (p < .05), while Bcl-2 and Bax expression levels had no obvious change (p > .05). Altogether, the above results indicate that AQP8 is involved in oestrogen-mediated regulation of buffalo follicular development by regulating cell cycle progression and apoptosis of GCs.
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http://dx.doi.org/10.1111/rda.13921DOI Listing
February 2021

Research on region selection super resolution restoration algorithm based on infrared micro-scanning optical imaging model.

Sci Rep 2021 Feb 2;11(1):2852. Epub 2021 Feb 2.

Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, 130033, Jilin, China.

With spring up of infrared imaging related industry, infrared imaging technology has become mainstream development direction of intelligent photoelectrical detection due to its good concealment, wide detection range, high positioning accuracy, long distant penetration, light weight, little volume, low power dissipation and high solidity. However, the features of infrared dim-small target image such as less details and low SNR become bottleneck of infrared image application. How to enhance imaging effect of infrared dim-small target becomes research hotspot. Starting from the point of 'restoration as foundation', the theory and technology of infrared dim-small target super-resolution restoration by utilizing the theory and technology of super-resolution restoration are explored in this paper. This paper mainly focuses on the research of super-resolution restoration algorithm of infrared dim-small target based on infrared micro-scanning optical model. Aiming at solving super-resolution restoration problem of infrared dim-small target, the traditional super-resolution restoration algorithm is optimized and the improved algorithm is proposed. Meanwhile, infrared micro-scanning optical model is introduced to break theoretical limit of simple image processing algorithm. And the performance of infrared image super-resolution restoration is improved.
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http://dx.doi.org/10.1038/s41598-021-82119-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854731PMC
February 2021

Expression patterns of ZO-1/2 and their effects on porcine oocyte in vitro maturation and early embryonic development.

Theriogenology 2021 Feb 13;161:262-270. Epub 2020 Dec 13.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, 530005, PR China. Electronic address:

Zonula occludens (ZO)-1 and ZO-2 are involved in epithelial polarity maintenance, gene transcription, cell proliferation and tumor cell metastasis. Regulating ZO-1/2 expression influences the early embryonic development of mice, but whether they are involved in oocyte maturation is still poorly understood. In the present study, the expression patterns of ZO-1 and ZO-2 in porcine cumulus cells and oocytes matured in vitro and early embryos from parthenogenetic activation were detected by qRT-PCR or Western blot, and then their roles in porcine oocyte maturation and early embryo development were investigated by shRNA technology. ZO-1 and ZO-2 were found to be expressed in cumulus cells, oocytes and early embryos, while ZO-1α was expressed only in cumulus cells, morula and blastocysts. During in vitro maturation (IVM), the abundance of ZO-1 and ZO-2 in oocytes was significantly higher than that in cumulus cells at 0 h (P < 0.01), and their mRNA and protein levels displayed relatively higher expression at 0 and 18 h, respectively. Compared with the control groups, cumulus cell expansion, oocyte nucleus maturation, and subsequent cleavage were not influenced by treatment of the cumulus-oocyte complexes (COCs) with ZO-1-shRNA1, ZO-2-shRNA2 or combined ZO-1-shRNA1 and ZO-2-shRNA2 lentivirus (P > 0.05). However, the blastocyst rate was reduced by treatment of COCs with ZO-1-shRNA1 but not ZO-2-shRNA2. The total cell number of blastocysts was decreased by downregulation of ZO-1 and ZO-2 (P < 0.05). Downregulation of ZO-1 and ZO-2 also resulted in a significant decrease (P < 0.05) in the expression of Cx43, Cx45, PTX3 and PTGS2 in cumulus cells, Cx45, BMP15, ZP3 and C-KIT in MII oocytes, and Nanog in blastocysts, with the exception of HAS2 expression in cumulus cells and Oct4 expression in blastocysts (P > 0.05). Altogether, the above results indicate that ZO-1 and ZO-2 display similar expression patterns during porcine oocyte IVM and are critical to porcine oocyte maturation and early embryonic development.
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http://dx.doi.org/10.1016/j.theriogenology.2020.12.009DOI Listing
February 2021

Editorial: tenofovir alafenamide fumarate-a new bullet to prevent mother-to-child transmission of hepatitis B virus. Authors' reply.

Aliment Pharmacol Ther 2020 12;52(11-12):1746-1747

Department of Infectious Disease, Xiangya Hospital, Central South University, Hunan, China.

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http://dx.doi.org/10.1111/apt.16101DOI Listing
December 2020

CircRNAs and LncRNAs in Osteoporosis.

Differentiation 2020 Nov - Dec;116:16-25. Epub 2020 Oct 28.

Jiangxi University of Science and Technology, Ganzhou, 341000, China; State Key Laboratory of High Performance Complex Manufacturing, Central South University, Changsha, 410083, China. Electronic address:

Osteoporosis is a systemic bone disease with bone fragility and increased fracture risk. The non-coding RNAs (ncRNAs) have appeared as important regulators of cellular signaling and pertinent human diseases. Studies have demonstrated that circular RNAs (circRNAs) and long non-coding RNAs (lncRNAs) are involved in the progression of osteoporosis through a variety of pathways, and are considered as targets for the prophylaxis and treatment of osteoporosis. Based on an in-depth understanding of their roles and mechanisms in osteoporosis, we summarize the functions and molecular mechanisms of circRNAs and lncRNAs involved in the progression of osteoporosis and provide some new insights for the prognosis, diagnosis and treatment of osteoporosis.
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http://dx.doi.org/10.1016/j.diff.2020.10.002DOI Listing
October 2020

Nonlinear optical absorption features in few-layered hybrid TiC(OH)/TiCF MXene for optical pulse generation in the NIR region.

Opt Express 2020 Oct;28(21):31499-31509

In the present work, we report the structural properties of the two dimensional (2D) few-layered TiC(OH)/TiCF hybrid MXene synthesized via the HF acid etching method. Various characterizations were exploited to demonstrate the 2D layered structural properties of the hybrid MXene membranes. The density functional theory (DFT) simulation indicated the hybrid MXene possessed the small enough band gap, which could benefit the nonlinear optical applications in the infrared region. By the conventional open-aperture Z-scan technique, typical nonlinear saturable features were measured. Consequently, the hybrid MXene membranes exhibited the excellent saturable absorption properties at 1 and 1.3 µm. As a saturable absorber, passively Q-switched Nd:YVO lasers with the prepared hybrid MXene membranes were realized at 1 and 1.3 µm, respectively, producing the stable Q-switching pulse train with a shortest duration of 130 ns.
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http://dx.doi.org/10.1364/OE.406035DOI Listing
October 2020

Efficacy and safety of tenofovir alafenamide fumarate for preventing mother-to-child transmission of hepatitis B virus: a national cohort study.

Aliment Pharmacol Ther 2020 10 27;52(8):1377-1386. Epub 2020 Aug 27.

Beijing, China.

Background: Data on tenofovir alafenamide fumarate (TAF) for preventing mother-to-child transmission of hepatitis B virus (HBV) are lacking.

Aims: To investigate the efficacy and safety of TAF therapy for preventing hepatitis B mother-to-child transmission.

Methods: Mothers with chronic HBV infection, positive for hepatitis B e-antigen and with HBV DNA >200 000 IU/mL received TAF for preventing mother-to-child transmission were enrolled retrospectively from multiple centres with data collection on mother-infant dyads up to postpartum week 24-28. Primary measurements were the mother-to-child transmission rate and infants' malformation rate. Secondary assessments included maternal HBV DNA reduction at delivery, and maternal or infant adverse events during follow up.

Results: Among 71 mothers enrolled, the mean (±SD) age was 30.3 (±2.2) years. TAF was initiated during the second or third trimester and continued to delivery with a mean (±SD) duration of 12.8 (±4.0) weeks. At delivery, 85.9% (61/71) of the mothers achieved HBV DNA <200 000 IU/L. Seventy-three infants (two sets of twins) were born from mothers treated with TAF and none had congenital defects or malformations. All infants received HBV immunoglobulin and vaccine at birth with additional HBV vaccinations at one and six months. At age 24-28 weeks, all infants had negative hepatitis B surface antigen and undetectable levels of HBV DNA (<100 IU/mL). Body weight, height, and head circumferences were comparable to national standards for physical development. No severe adverse effects were reported in either mothers or infants.

Conclusions: TAF for highly viraemic mothers effectively prevented mother-to-child transmission of hepatitis B. There were no safety concerns for either mothers or infants with 24-28 weeks of follow up.
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http://dx.doi.org/10.1111/apt.16043DOI Listing
October 2020

Advances in the occurrence and biotherapy of osteoporosis.

Biochem Soc Trans 2020 08;48(4):1623-1636

Jiangxi University of Science and Technology, Ganzhou 341000, China.

Osteoporosis (OP) is a bone metabolic disease, is characterized by degeneration of bone structure and decreased bone mass. It happens in more than 1/3 women and 1/5 men of over than 50 years old, which affects the health and lives of people. The main mechanism of OP is mainly that the dynamic balance between the bone formation and resorption is broken, so that bone resorption is more than bone formation. It is prone to result in bone metabolism disorder. There are many precipitating factor such as elder age, low hormone level, genetic factors and bad hobbies. At the same time, the occurrence of the OP and its complications has different degrees of impact on people's quality of life. Based on the current understanding of the OP, we summarized the etiology, current clinical drugs and potential targeting therapy for OP. Although the research have made many progress in explore what is the novel mechanism and how to improve the effect, there are still many problems in the treatment method that limit its application prospects and need to be solved. In this review, we mainly focus on the mechanism of OP and related research on the targeted treatment of OP. Hopefully, our summary will provide a reference to develop some novel strategies for the target therapy of OP.
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http://dx.doi.org/10.1042/BST20200005DOI Listing
August 2020

Difference in binocular rivalry rate between major depressive disorder and generalized anxiety disorder.

Behav Brain Res 2020 08 24;391:112704. Epub 2020 May 24.

Department of Neurology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, China; Anhui Province Key Laboratory of Cognition and Neuropsychiatric Disorders, Hefei, Anhui Province, China. Electronic address:

Binocular rivalry (BR) occurs when monocular images are presented simultaneously to corresponding regions of the two eyes. Rather than forming a stable composite, perception alternates spontaneously between each monocular view. Over recent decades, considerable interest in examining BR in psychiatric populations has demonstrated clear differences in the dynamics of BR when compared to healthy populations. However, the available data with respect to rivalry rates in depression and anxiety are limited, and previous studies have shown inconsistent results. Also, depression and anxiety are highly comorbid and can be difficult to distinguish. Therefore, we aimed to investigate whether patients with depression and anxiety have abnormal rivalry rates and whether rivalry rates differ between these conditions. Thirty-five patients with major depressive disorder (MDD), 30 patients with generalized anxiety disorder (GAD), and 30 control participants matched on sex, age and education were recruited. Our results showed MDD participants had slower BR rates than controls and GAD, and controls were significantly slower than GAD. Our findings raise prospects that BR could be a promising behavioral tool to differentiate depressive and anxiety disorders.
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http://dx.doi.org/10.1016/j.bbr.2020.112704DOI Listing
August 2020

Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression.

Int J Biol Sci 2020 15;16(11):1941-1953. Epub 2020 Apr 15.

NHC Key Laboratory of Carcinogenesis of Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine; School of basic Medical Science, Central South University, Changsha, Hunan 410013, China.

Ovarian cancer (OC) is one of the malignant tumors that seriously threaten women's health, with the highest mortality rate in gynecological malignancies. The prognosis of patients with advanced OC is still poor, and the 5-year survival rate is only 20-30%. Therefore, how to improve the early diagnosis rate and therapeutic effect are urgent for patients with OC. In this research, we found that Lin28A can promote the expression of stem cell marker molecules CD133, CD44, OCT4 and Nanog. We later confirmed that Lin28A can enrich the mRNA of ras-related nuclear protein (RAN) and heat shock factor binding protein 1 (HSBP1) through RIP assay, and that Lin28A can regulate their protein expression. We also identified that RAN and HSBP1 are highly expressed in OC tissues, and that they are significantly positively correlated with the expression of Lin28A and negatively correlated with the survival prognosis of OC patients. After stable knockdown of RAN or HSBP1 in OC cells with high expression of Lin28A, the expression of the stem cell marker molecules such as OCT4, CD44 and Nanog are reduced. And after knocking down of RAN or HSBP1 in Lin28A highly expressed OC cells, the survival and invasion of OC cells and tumor size of OC xenograft in nude mice were markedly inhibited and apoptosis was increased. Our data also showed that knock down of RAN or HSBP1 can inhibit the invasion ability of OC cells by decreasing the expression of N-cadherin, Vimentin and promoting the expression of E-cadherin. Meanwhile, knockdown of RAN or HSBP1 induced cell apoptosis by inhibiting the expression of PARP. Our results indicated that Lin28A could regulate the biological behaviors in OC cells through RAN/HSBP1. These findings suggest that Lin28A/RAN/HSBP1 can be used as a marker for diagnosis and prognosis of OC patients, and RAN/HSBP1 may be a potential new target for gene therapy of OC.
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http://dx.doi.org/10.7150/ijbs.43504DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211169PMC
April 2020

LINC01116 promotes tumor proliferation and neutrophil recruitment via DDX5-mediated regulation of IL-1β in glioma cell.

Cell Death Dis 2020 05 1;11(5):302. Epub 2020 May 1.

Department of Neurology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.

Tumor-associated neutrophils (TANs) are important inflammatory infiltrating cells in the tumor microenvironment and are closely related to the development of human tumor. However, the underlying mechanism of TANs recruiting to glioma remains unknown. Herein, we identified that LINC01116 was significantly upregulated in glioma, and positively correlated with clinical malignancy and survival prognosis. LINC01116 regulated the progression of glioma in vitro and in vivo. RNA-seq analysis demonstrated that LINC01116 knockdown affected the expression of IL-1β, which promoted glioma proliferation and neutrophil recruitment. Furthermore, the co-culture of glioma cells and neutrophils showed that the accumulation of TANs promoted tumor proliferation via producing a host of cytokines. Mechanistically, LINC01116 activated IL-1β expression by recruiting the transcriptional regulator DDX5 to the IL-1β promoter. Our findings reveal that LINC01116 can promote glioma proliferation and neutrophil recruitment by regulating IL-1β, and may be served as a novel target for glioma therapy and prognosis.
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http://dx.doi.org/10.1038/s41419-020-2506-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195423PMC
May 2020

Linc02349 promotes osteogenesis of human umbilical cord-derived stem cells by acting as a competing endogenous RNA for miR-25-3p and miR-33b-5p.

Cell Prolif 2020 May 29;53(5):e12814. Epub 2020 Apr 29.

Institute of Bioadditive Manufacturing, Jiangxi University of Science and Technology, Nanchang, China.

Objectives: Increasing evidences suggest that inducing mesenchymal stem cells to differentiate into osteoblasts has been as an especially important component in the prevention and therapy for degenerative bone disease. Here, we identify a novel lncRNA, linc02349, which increases significantly during osteogenic differentiation.

Materials And Methods: Human umbilical cord-derived stem cells (hUC-MSCs) and dental pulp mesenchymal stem cells were used. Overexpression and knockdown of linc02349 in cell lines were generated using lentiviral-mediated gene delivery method. Bioinformatics prediction, Ago2-RIP assay and dual-luciferase reporter system were employed to examine miRNA which interacts with linc02349. The RNA FISH assay was performed to identify the subcelluar location of linc02349. Alizarin Red S staining, ALP staining and qPCR were applied to identify the osteogenic differentiation. The potential linc02349-regulated genes, miR-25-3p and miR-33b-5p, were explored by ChIP, RIP and Western blotting assays. Micro-CT was used to measure the osteogenic content in bone formation assay in vivo.

Results: Linc02349 overexpression improves osteogenic differentiation by in vitro and in vivo analysis. Mechanistically, linc02349 acts as a molecular sponge for miR-25-3p and miR-33b-5p to control expression abundance of SMAD5 and Wnt10b, respectively, which eventually activated Dlx5/OSX pathway and hence promoted osteogenic differentiation. In addition, we revealed that STAT3 interacts with linc02349 promoter region and positively regulates the linc02349 transcriptional activity.

Conclusion: These findings identify that linc02349 modulates the osteogenic differentiation through acting as a sponge RNA of miR-25-3p and miR-33b-5p and regulating SMAD5 and Wnt10b, and proposed a new interaction between STAT3 and linc02349, which could be a potential target in the process the osteogenesis of hUC-MSCs for future clinical application.
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http://dx.doi.org/10.1111/cpr.12814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260076PMC
May 2020

Fatsia Japonica-Derived Hierarchical Porous Carbon for Supercapacitors With High Energy Density and Long Cycle Life.

Front Chem 2020 20;8:89. Epub 2020 Feb 20.

Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Materials Science and Engineering, Nanjing Forestry University, Nanjing, China.

Fatsia Japonica seed, which is mainly composed of glucose, has potential as a porous carbon matrix precursor for supercapacitors that can achieve high-value utilization. Cost-effective hierarchical porous carbon materials (HPC) were prepared from Fatsia Japonica by annealing at high temperature. The pore size and distribution of the HPC can be precisely controlled and adjusted by altering the activation temperature. The HPC obtained at 600°C showed favorable features for electrochemical energy storage, with a surface area of 870.3 m/g. The HPC for supercapacitors (a three-electrode system) exhibited good specific capacitance of 140 F/g at a current density of 1 A/g and a long cycling life stability (87.5% remained after 10,000 cycles). In addition, the HPC electrode showed an excellent energy density of 23 Wh/Kg. Such hierarchical porous biomass-derived carbon would be a good candidate for application in the electrodes of supercapacitors due to its simple preparation process and the outstanding electrochemical performance.
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http://dx.doi.org/10.3389/fchem.2020.00089DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044675PMC
February 2020

Stimulated Brillouin scattering of backward stimulated Raman scattering.

Sci Rep 2020 Feb 26;10(1):3492. Epub 2020 Feb 26.

Institute of Applied Physics and Computational Mathematics, Beijing, 100094, China.

The rescattering of backward stimulated Raman scattering (BSRS) by stimulated Brillouin scattering (SBS) is found in the high electron density region by relativistic Vlasov-Maxwell simulation and particle-in-cell (PIC) simulation, where the BSRS is in the regime of absolute instability and dominates in all the scatterings. Both one dimension (1D) Vlasov simulation and two dimension (2D) PIC simulation have been given to verify that there exists SBS of BSRS in the regime of absolute instability for BSRS. The SBS of BSRS will be even stronger than forward stimulated Raman scattering (FSRS) and SBS in regime of absolute instability for BSRS. Thus, besides Langmuir decay instability and laser energy absorption, the SBS of BSRS is also an important saturation mechanism of BSRS in high electron density region.
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http://dx.doi.org/10.1038/s41598-020-59727-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7044175PMC
February 2020

Liver Stiffness Measurement Can Reflect the Active Liver Necroinflammation in Population with Chronic Liver Disease: A Real-world Evidence Study.

J Clin Transl Hepatol 2019 Dec 11;7(4):313-321. Epub 2019 Dec 11.

Department of Microbiology & Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.

Non-invasive evaluation of liver necroinflammation in patients with chronic liver disease is an unmet need in clinical practice. The diagnostic accuracy of transient elastography-based liver stiffness measurement (LSM) for liver fibrosis could be affected by liver necroinflammation, the latter of which could intensify stiffness of the liver. Such results have prompted us to explore the diagnosis potential of LSM for liver inflammation. Three cross-sectional cohorts of liver biopsy-proven chronic liver disease patients were enrolled, including 1417 chronic hepatitis B (CHB) patients from 10 different medical centers, 106 non-alcoholic steatohepatitis patients, and 143 patients with autoimmune-related liver diseases. Another longitudinal cohort of 14 entecavir treatment patients was also included. The receiver operating characteristic (ROC) curve was employed to explore the diagnostic value of LSM. In CHB patients, LSM value ascended with the increased severity of liver necroinflammation in patients with the same fibrosis stage. Such positive correlation between LSM and liver necroinflammation was also found in non-alcoholic steatohepatitis and autoimmune-related liver diseases populations. Furthermore, the ROC curve exhibited that LSM could identify moderate and severe inflammation in CHB patients (area under the ROC curve as 0.779 and 0.838) and in non-alcoholic steatohepatitis patients (area under the ROC curve as 0.826 and 0.871), respectively. Such moderate diagnostic value was also found in autoimmune-related liver diseases patients. In addition, in the longitudinal entecavir treated CHB cohort, a decline of LSM values was observed in parallel with the control of inflammatory activity in liver. Our study implicates a diagnostic potential of LSM to evaluate the severity of liver necroinflammation in chronic liver disease patients.
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http://dx.doi.org/10.14218/JCTH.2019.00040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6943212PMC
December 2019

Dietary meat intake and risk of asthma in children: evidence from a meta-analysis.

Medicine (Baltimore) 2020 Jan;99(1):e18235

Department of Respiratory Medicine, The Second Affiliated Hospital of Dalian Medical University, Dalian 116000, Liaoning, China.

Background: Many studies have been reported that dietary meat intake may be associated with the risk of asthma in children, but the results are inconsistent. Therefore, we performed a meta-analysis to evaluate the effect of meat on the risk of asthma in children.

Methods: The databases PubMed, Embase, and Web of Science were searched. Pooled odds ratios (OR) and 95% confidence intervals (CI) were calculated with random-effect model using Stata software.

Results: A total of 9 articles were included in this meta-analysis. Results from our study suggest that dietary meat intake 3 or more times per week compared with never/occasionally intake has no significant association with asthma risk among children (OR = 1.27, 95% CI = 0.80-2.01, P = .308). Similarly, daily intake of meat did not affect the risk of asthma in children when compared with never/occasionally intake (OR = 1.13, 95% CI = 0.93-1.37, P = 0.234). In addition, no publication biases were detected in our meta-analysis.

Conclusion: Dietary meat intake most probably is not a risk factor for asthma in children. Due to some limitations that exist in our study, more studies are needed to further assess the association between meat intake and asthma risk in children.
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http://dx.doi.org/10.1097/MD.0000000000018235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946446PMC
January 2020

Broadband Nonlinear Optical Response of InSe Nanosheets for the Pulse Generation From 1 to 2 μm.

ACS Appl Mater Interfaces 2019 Dec 13;11(51):48281-48289. Epub 2019 Dec 13.

School of Information Science and Engineering , Shandong University , Qingdao 266237 , China.

Few-layered InSe nanosheets were fabricated by the simple liquid-phase exfoliation method. The morphology and crystal structure features of InSe nanosheet sample were characterized comprehensively. The photoluminescence (PL) spectrum indicated that the liquid-phase exfoliated InSe nanosheets contained variously layered nanoflakes, where eight layers nanosheets dominate. In addition, the first-principle simulation was carried out to describe the electron density of states (DOS) and the electronic band structures. Moreover, the few-layered InSe nanosheets performed excellent nonlinear absorption properties in a broad spectral band. As an application, the stable passively Q-switched (PQS) lasers with few-layered InSe nanosheets saturable absorbers (SAs) were realized with the operating wavelengths at 1.06, 1.34, and 1.91 μm. The shortest pulse durations were 599, 520, and 210 ns, respectively. Our results confirmed that the few-layered InSe nanosheets could be an excellent candidate for pulsed lasers in wide spectral bands.
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http://dx.doi.org/10.1021/acsami.9b18632DOI Listing
December 2019

LncRNA ODIR1 inhibits osteogenic differentiation of hUC-MSCs through the FBXO25/H2BK120ub/H3K4me3/OSX axis.

Cell Death Dis 2019 12 11;10(12):947. Epub 2019 Dec 11.

Jiangxi University of Science and Technology, Ganzhou, 341000, China.

Long noncoding RNAs (lncRNAs) have been demonstrated to be important regulators during the osteogenic differentiation of mesenchymal stem cells (MSCs). We analyzed the lncRNA expression profile during osteogenic differentiation of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) and identified a significantly downregulated lncRNA RP11-527N22.2, named osteogenic differentiation inhibitory lncRNA 1, ODIR1. In hUC-MSCs, ODIR1 knockdown significantly promoted osteogenic differentiation, whereas overexpression inhibited osteogenic differentiation in vitro and in vivo. Mechanistically, ODIR1 interacts with F-box protein 25 (FBXO25) and facilitates the proteasome-dependent degradation of FBXO25 by recruiting Cullin 3 (CUL3). FBXO25 increases the mono-ubiquitination of H2BK120 (H2BK120ub) which subsequently promotes the trimethylation of H3K4 (H3K4me3). Both H2BK120ub and H3K4me3 form a loose chromatin structure, inducing the transcription of the key transcription factor osterix (OSX) and increasing the expression of the downstream osteoblast markers, osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP). In summary, ODIR1 acts as a key negative regulator during the osteogenic differentiation of hUC-MSCs through the FBXO25/H2BK120ub/H3K4me3/OSX axis, which may provide a novel understanding of lncRNAs that regulate the osteogenesis of MSCs and a potential therapeutic strategy for the regeneration of bone defects.
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http://dx.doi.org/10.1038/s41419-019-2148-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906393PMC
December 2019

A new triterpenoid and other constituents with cytotoxic activity from the roots of L.

Nat Prod Res 2019 Dec 4:1-5. Epub 2019 Dec 4.

Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University College of Pharmacy, Yanji, P.R. China.

L. is a traditional herbal plant that belongs to the genus and the family Rosaceae. A new ursane-type triterpenoid, 3-oxo-urs-11, 13(18)-dien-19, 28-olide (), two known ursane-type triterpenoids (-) and three known oleanane-type triterpenoids (, -) were isolated from the roots of by silica gel column and MPLC. Their structures were identified by interpretation of spectroscopic data (1 D NMR, 2 D NMR, HR-ESI-MS) and comparison with those reported in the literature. Compound was isolated from the Rosaceae family, compounds - were obtained from the genus , and compound was obtained from the for the first time. Additionally, all of the isolated compounds were evaluated for their cytotoxic activity against three human cancer cells. Compound showed better cytotoxic activity against A549, HeLa, SK-Hep1 cells than the other compounds with IC values of 48.58 ± 1.88, 47.84 ± 2.01, 42.31 ± 2.43 μM, respectively.
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http://dx.doi.org/10.1080/14786419.2019.1693569DOI Listing
December 2019

Salidroside mitigates skeletal muscle atrophy in rats with cigarette smoke-induced COPD by up-regulating myogenin and down-regulating myostatin expression.

Biosci Rep 2019 11;39(11)

Department of Respiratory Medicine, Institute of Respiratory Diseases, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China.

Objectives: The present study aimed at investigating the therapeutic effect of Salidroside on skeletal muscle atrophy in a rat model of cigarette smoking-induced chronic obstructive pulmonary disease (COPD) and its potential mechanisms.

Methods: Male Wistar rats were randomized, and treated intraperitoneally (IP) with vehicle (injectable water) or a low, medium or high dose of Salidroside, followed by exposure to cigarette smoking daily for 16 weeks. A healthy control received vehicle injection and air exposure. Their lung function, body weights and gastrocnemius (GN) weights, grip strength and cross-section area (CSA) of individual muscular fibers in the GN were measured. The levels of TNF-α, IL-6, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) in serum and GN tissues as well as myostatin and myogenin expression in GN tissues were measured.

Results: In comparison with that in the healthy control, long-term cigarette smoking induced emphysema, significantly impaired lung function, reduced body and GN weights and CSA values in rats, accompanied by significantly increased levels of TNF-α, IL-6 and MDA, but decreased levels of SOD and GSH in serum and GN tissues. Furthermore, cigarette smoking significantly up-regulated myostatin expression, but down-regulated myogenin expression in GN tissues. Salidroside treatment decreased emphysema, significantly ameliorated lung function, increased antioxidant, but reduced MDA, IL-6 and TNF-α levels in serum and GN tissues of rats, accompanied by decreased myostain, but increased myogenin expression in GN tissues.

Conclusion: Salidroside mitigates the long-term cigarette smoking-induced emphysema and skeletal muscle atrophy in rats by inhibiting oxidative stress and inflammatory responses and regulating muscle-specific transcription factor expression.
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http://dx.doi.org/10.1042/BSR20190440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879355PMC
November 2019

Netrin‑1 interference potentiates epithelial‑to‑mesenchymal transition through the PI3K/AKT pathway under the hypoxic microenvironment conditions of non‑small cell lung cancer.

Int J Oncol 2019 Apr 15;54(4):1457-1465. Epub 2019 Feb 15.

Department of Respiratory Medicine, Τhe Second Hospital Affiliated to Dalian Medical University, Dalian, Liaoning 116027, P.R. China.

Netrin‑1 is overexpressed in several types of cancer. However, whether netrin‑1 can potentiate hypoxia‑induced tumor progression in lung cancer has not been reported to date. Thus, the objective of the present study was to investigate whether netrin‑1 regulates cancer cell migration and invasion under hypoxic conditions in lung cancer and explore the underlying mechanism. A three‑dimensional microfluidic chip was used to observe real‑time changes in cancer cells, and cobalt chloride (CoCl2) was used to simulate a hypoxic microenvironment. Netrin‑1 siRNA was employed in the A549 and PC9 cell lines to downregulate the expression of netrin‑1. Microfluidic chip, wound healing and Transwell assays were used to examine cell migration and invasion. The expression levels of E‑cadherin and vimentin were detected by western blotting. The data demonstrated that netrin‑1 mediated epithelial‑to‑mesenchymal transition (EMT) of A549 and PC9 cells in vitro, which may be associated with the phosphoinositide 3 kinase/AKT pathway. This effect of netrin‑1 on the EMT was not observed in the normoxic microenvironment. In this retrospective study, netrin‑1 concentrations were evaluated in serum obtained from patients with non‑small cell lung cancer (NSCLC) and compared with healthy control samples by quantitative enzyme‑linked immunosorbent analysis. The serum concentration of netrin‑1 was found to be significantly higher in NSCLC patients compared with that in healthy donors. Taken together, the findings of the present study highlight a novel role for netrin‑1 in tumor development under hypoxia in NSCLC and provide further evidence for the use of netrin‑1 as a therapeutic target.
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http://dx.doi.org/10.3892/ijo.2019.4716DOI Listing
April 2019

Self-Organized Kilotesla Magnetic-Tube Array in an Expanding Spherical Plasma Irradiated by kHz Femtosecond Laser Pulses.

Phys Rev Lett 2018 Dec;121(25):255002

State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, People's Republic of China.

By using a millijoule kHz femtosecond laser pulse to irradiate a preformed expanding spherical plasma, which is driven by a prepulse with intensity of 1×10^{14}  W/cm^{2}, we observe fast-electron-mediated filamentary structures and an accompanying self-organized magnetic-tube array with 2000 T via time-resolved magneto-optical polarization rotation measurements. We reveal that these periodical filamentary structures predominantly originate from ejected energetic electron flows from the inner denser region of the spherical plasma, which will induce the electron Weibel instability and magnetic field organization and amplification in the expanding plasma in 2 ps. These results open new paths to investigate amplification of intense magnetic fields and the radiation signature from gamma-ray bursts just by means of a much smaller and robust experimental platform.
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http://dx.doi.org/10.1103/PhysRevLett.121.255002DOI Listing
December 2018

Short article: Sequence variations of PKHD1 underlie congenital hepatic fibrosis in a Chinese family.

Eur J Gastroenterol Hepatol 2019 03;31(3):363-367

The Research Center for Medical Genomics, Key Laboratory of Cell Biology, Ministry of Public Health, Key Laboratory of Medical Cell Biology, Ministry of Education, College of Basic Medical Science, China Medical University.

Objective: Congenital hepatic fibrosis (CHF) is a developmental disorder of the portobiliary system characterized by hepatic fibrosis, portal hypertension, and renal cystic disease. The aim of our study was to identify the disease-causing gene of a Chinese family with CHF.

Patients And Methods: Whole-exome sequencing was performed in the family with CHF and variants were confirmed by Sanger sequencing. Online bioinformatics tools were used to evaluate the pathogenicity of the missense variants. Liver specimens were reviewed to confirm the histopathological diagnosis.

Results: The compound heterozygous variants c.7994T>C, p.(Leu2665Pro) and c.8518C>T, p.(Arg2840Cys) in PKHD1 were identified in a Chinese family with CHF by whole-exome sequencing. Liver histomorphology was reviewed to confirm the diagnosis of CHF.

Conclusion: We have identified variations in PKHD1 in a Chinese family with CHF. Our study extends the mutation spectrum of CHF and provides information for genetic counseling of patients' family members.
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http://dx.doi.org/10.1097/MEG.0000000000001295DOI Listing
March 2019

The protective role of verbenalin in rat model of focal cerebral ischemia reperfusion.

Saudi J Biol Sci 2018 Sep 13;25(6):1170-1177. Epub 2017 Oct 13.

Department of Pharmacology, Henan University of TCM, Zhengzhou 450046, China.

To investigate the protective mechanism of verbenalin on cerebral ischemia-reperfusion injury. Middle cerebral artery occlusion in the left hemisphere was induced in rats by filament insertion, and rat model of focal cerebral ischemia-reperfusion was established. The high, medium and low dose of verbenalin groups were injected in the tail vein of corresponding drugs 10 min before reperfusion, and submitted for 22 h of reperfusion after the operation. Mortality rate was then calculated, and neurological deficits of rats were scored. The serum of rats was got to determine the S-100β protein level, and the brain tissue was removed to determine the levels of Bax, Bcl-2, Caspase-3 and ATPase. TTC staining was performed on the brain tissue to calculate the percentage of cerebral infarct size. Changes in brain tissue morphology were observed. Rat model of focal cerebral ischemia-reperfusion was successfully replicated. In groups that have taken different doses of verbenalin, the mortality rate, neurological deficit score and the percentage of cerebral infarction size were significantly reduced, and the levels of Bax, Caspase-3, S-100β level of the serum in the brain tissue were also significantly reduced. Increases in the levels of Bcl-2 and ATPase in brain tissue and improvement of pathological damage of hippocampus and cortex were observed. Verbenalin can inhibit the expression of apoptosis genes, promote the expression of anti-apoptosis genes, improve brain microcirculation and energy metabolism, hence reducing cerebral ischemia-reperfusion injury.
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http://dx.doi.org/10.1016/j.sjbs.2017.10.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117236PMC
September 2018

A novel missense mutation of CRYGS underlies congenital cataract in a Chinese family.

Gene 2018 Oct 28;675:9-14. Epub 2018 Jun 28.

The Research Center for Medical Genomics, Key Laboratory of Cell Biology, Ministry of Public Health, Key Laboratory of Medical Cell Biology, Ministry of Education, College of Basic Medical Science, China Medical University, Shenyang, China. Electronic address:

Congenital cataract is a clinically and genetically heterogeneous disease. In this study, we examined a five-generation Chinese family with autosomal dominant nuclear congenital cataracts by whole exome sequencing. A novel heterozygous missense mutation c.199T>A, p.(Tyr67Asn) in CRYGS was identified in this family. The p.(Tyr67Asn) substitution was predicted to decrease the local hydrophobicity and affect the three-dimensional structure of γS-crystallin, and resulted in a portion of mutant protein translocation from the cytoplasm to cell membrane. Our observations expand the mutation spectrum of CRYGS and provide further evidence for the genetic basis and molecular mechanism of congenital cataract.
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http://dx.doi.org/10.1016/j.gene.2018.06.100DOI Listing
October 2018

Clinical and genetic characteristics of Chinese patients with familial or sporadic pediatric cataract.

Orphanet J Rare Dis 2018 06 18;13(1):94. Epub 2018 Jun 18.

The Research Center for Medical Genomics, Key Laboratory of Cell Biology, Ministry of Public Health, Key Laboratory of Medical Cell Biology, Ministry of Education, College of Basic Medical Science, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang, 110122, China.

Background: Pediatric cataract is a clinically and genetically heterogeneous disease which is a significant cause of lifelong visual impairment and treatable blindness. Our study aims to investigate the genotype spectrum in a group of Chinese patients with pediatric cataract.

Methods: We enrolled 39 families with pediatric cataract from October 2015 to April 2016. DNA samples of the probands were analyzed by target next-generation sequencing. Variants were validated using Sanger sequencing in the probands and available family members.

Results: In our cohort of 39 cases with different types of pediatric cataract, 23 cases were found to harbor putative pathogenic variants in 15 genes: CRYAA, CRYBA1, CRYBA4, CRYBB1, CRYGC, CRYGD, MIP, GCNT2, IARS2, NHS, BCOR, BFSP2, FYCO1, MAF, and PAX6. The mutation detection rates in the familial and sporadic cases were 75 and 47.8%, respectively. Of the 23 causative variants, over half were novel.

Conclusions: This is a rare report of systematic mutation screening analysis of pediatric cataract in a comparably large cohort of Chinese patients. Our observations enrich the mutation spectrum of pediatric cataract. Next-generation sequencing provides significant diagnostic information for pediatric cataract cases, especially when considering sporadic and subtle syndromal cases.
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http://dx.doi.org/10.1186/s13023-018-0828-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6006596PMC
June 2018

Mutations in mevalonate pathway genes in patients with familial or sporadic porokeratosis.

J Dermatol 2018 Jul 3;45(7):862-866. Epub 2018 May 3.

The Research Center for Medical Genomics, Key Laboratory of Cell Biology, Ministry of Public Health, Key Laboratory of Medical Cell Biology, Ministry of Education, College of Basic Medical Science, China Medical University, Shenyang, China.

Porokeratosis comprises heterogeneous keratinization disorders that are characterized by one or more atrophic patches surrounded by a ridge-like cornoid lamella. In this study, we evaluated seven families affected by porokeratosis and five sporadic patients of the disease in a Chinese population. We performed Sanger sequencing of exons and flanking intron-exon boundaries of mevalonate pathway genes (MVD, MVK, PMVK and FDPS) and of SLC17A9. In five familial and three sporadic patients, we detected six variations, including four novel mutations (MVD c.1A>G; p.Met1?, c.916G>A; p.Ala306Thr, c.1013+1G>A, and PMVK c.65A>G; p.Lys22Arg) and two recurrent mutations (MVD c.746T>C; p.Phe249Ser, and MVK c.1028T>C; p.Leu343Pro). We then applied I-TASSER and iGEMDOCK to assess these variants for probable functional impacts. The findings of this study extend the mutation spectrum of porokeratosis and provide further evidence for the genetic basis of this disease.
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http://dx.doi.org/10.1111/1346-8138.14343DOI Listing
July 2018

Exome sequencing reveals the genetic landscape and frequent inactivation of PCDHB3 in Chinese rectal cancers.

J Pathol 2018 06 20;245(2):222-234. Epub 2018 Apr 20.

Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangzhou, PR China.

Colorectal cancer (CRC) is the third most common cancer worldwide, with more than 1.3 million new cases and 690 000 deaths each year. In China, the incidence of CRC has increased dramatically due to dietary and lifestyle changes, to become the fifth leading cause of cancer-related death. Here, we performed whole-exome sequencing in 50 rectal cancer cases among the Chinese population as part of the International Cancer Genome Consortium research project. Frequently mutated genes and enriched pathways were identified. Moreover, a previously unreported gene, PCDHB3, was found frequently mutated in 5.19% cases. Additionally, PCDHB3 expression was found decreased in 81.6% of CRC tissues and all eight CRC cell lines tested. Low expression and cytoplasmic localization of PCDHB3 predict poor prognosis in advanced CRC. Copy number decrease and/or CpG island hypermethylation contributes to the pervasive decreased expression of PCDHB3. PCDHB3 inhibits CRC cell proliferation, migration, and epithelial-mesenchymal transition. The tumor-suppressive effects of PCDHB3 are partially due to inhibition of NF-κB transcriptional activity through K63 deubiquitination of p50 at lysine 244/252, which increases the binding affinity of inactive p50 homodimer to κB DNA, resulting in competitive inhibition of the transcription of NF-κB target genes by p65 dimers. Our study identified PCDHB3 as a novel tumor suppressor in CRC via inhibition of the NF-κB pathway, and its expression and localization may serve as prognostic markers for advanced CRC. Copyright © 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
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http://dx.doi.org/10.1002/path.5073DOI Listing
June 2018

An Overview of Long Noncoding RNAs Involved in Bone Regeneration from Mesenchymal Stem Cells.

Stem Cells Int 2018 28;2018:8273648. Epub 2018 Jan 28.

School of Energy and Mechanical Engineering, Jiangxi University of Science and Technology, Nanchang, Jiangxi, China.

Bone regeneration is very important for the recovery of some diseases including osteoporosis and bone fracture trauma. It is a multiple-step- and multiple-gene-involved complex process, including the matrix secretion and calcium mineralization by osteoblasts differentiated from mesenchymal stem cells (MSCs) and the absorption of calcium and phosphorus by osteoclasts differentiated from hematopoietic stem cells. Long noncoding RNAs (lncRNAs) are a family of transcripts longer than 200 nt without or with very low protein-coding potential. Recent studies have demonstrated that lncRNAs are widely involved in the regulation of lineage commitment and differentiation of stem cells through multiple mechanisms. In this review, we will summarize the roles and molecular mechanism of lncRNAs including , , , , , , , and in osteogenesis ossification; lncRNA and , , and in chondrogenic differentiation; and lncRNA in osteoclast differentiation. These findings will facilitate the development and application of novel molecular drugs which regulate the balance of bone formation and absorption.
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http://dx.doi.org/10.1155/2018/8273648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5829309PMC
January 2018