Publications by authors named "Lifeng Zhang"

292 Publications

Comparing Prognosis Associated with Partial Cystectomy and Trimodal Therapy for Muscle-Invasive Bladder Cancer Patients.

Urol Int 2021 Oct 14:1-12. Epub 2021 Oct 14.

Department of Urology, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, China.

Objective: This study aimed to compare the survival outcomes between trimodal therapy (TT) and partial cystectomy (PC) in muscle-invasive bladder cancer (MIBC) patients.

Methods: The data of 13,096 patients with MIBC diagnosed between 2004 and 2015 were retrieved from the Surveillance, Epidemiology, and End Results database. Among them, 4,041 patients underwent TT and 1,670 patients underwent PC. Propensity score matching was performed to balance the characteristics between the 2 treatment groups. A multivariate Cox regression analysis model and a competing risk model were used to evaluate overall survival (OS) and cancer-specific survival. Cumulative incidence survival curves were obtained using the Kaplan-Meier method.

Results: Results of multivariate Cox analysis before propensity score matching showed that the TT group had a 31% reduction in cause-specific survival relative to the PC group (HR: 0.69, 95% CI: 0.61-0.78, p < 0.001) and a 28% reduction in OS (HR: 0.72, 95% CI: 0.66-0.79, p < 0.001). After propensity score matching, the 2 groups yielded 972 patients, with 3-year cause-specific survival rates of 54.1% and 68.5% in the TT group and the PC group, respectively.

Conclusions: Patients who underwent PC had a better prognosis than those who received TT. In addition, for MIBC patients who required bladder-sparing therapy, advanced age (≥80 years), pathological type of squamous cell carcinoma, and tumor stage of T3-4, N2-3, and M1 were independent poor prognostic factors.
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http://dx.doi.org/10.1159/000518562DOI Listing
October 2021

Parental self-efficacy and family quality of life in parents of children with autism spectrum disorder in China: The possible mediating role of social support.

J Pediatr Nurs 2021 Nov 12. Epub 2021 Nov 12.

School of Nursing, Sun Yat-Sen University, Guangzhou, China. Electronic address:

Objective: This study explored the related factors of FQOL in parents of children with ASD and examined whether social support mediates the relationship between parental self-efficacy and FQOL in parents of children with ASD.

Design And Methods: Using a cross-sectional design, a total of 260 parents of children with ASD were recruited from September 2019 to November 2020. They completed the Beach Center Family Quality of Life Scale, the Parenting Sense of Competence Scale, and the Social Support Rating Scale.

Results: Parental self-efficacy and social support explained approximately 49.5% of the variance in FQOL. After controlling for the confounding influence of parents' education level, parental self-efficacy had a direct effect on FQOL (β = 0.292, SE = 0.108, P < 0.01) and an indirect effect on FQOL (β = 0.165, SE = 0.069, P < 0.01). Effects were mediated through social support, with partial mediating effects accounting for 36.11% of the total effect.

Conclusions: Both parental self-efficacy and social support are critical to promoting FQOL, and a partial mediating effect of social support was established.

Practical Implications: Interventions for families with children with ASD should focus on enhancing parental self-efficacy, followed by a perceived social support and FQOL prompt.
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http://dx.doi.org/10.1016/j.pedn.2021.10.014DOI Listing
November 2021

Computational Analysis of the Binding Mechanism of GenX and HSA.

ACS Omega 2021 Nov 19;6(43):29166-29170. Epub 2021 Oct 19.

Department of Chemistry, North Carolina Agricultural and Technical State University, Greensboro, North Carolina 27411, United States.

One PFOS alternative, ammonium 2,3,3,3-tetrafluoro-2-(heptafluoropropoxy) propanoate, known as GenX, was created to replace one of the original PFAS. This small and tough molecule has been found in surface water, groundwater, drinking water, rainwater, and air emissions in some areas in the United States. Recently, GenX has been shown to have an impact on several disease-related proteins in humans, and just like PFOS, it binds to human protein human serum albumin (HSA). In this paper, we reported four binding sites of GenX on HSA protein via docking and molecular dynamics simulation.
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http://dx.doi.org/10.1021/acsomega.1c04592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8567346PMC
November 2021

A survey of 3D object detection algorithms for intelligent vehicles development.

Artif Life Robot 2021 Nov 1:1-8. Epub 2021 Nov 1.

Kyushu Institute of Technology, Kitakyushu, Japan.

With the rapid development of Artificial Intelligent algorithms on Computer Vision, 2D object detection has greatly succeeded and been applied in various industrial products. In the past several years, the accuracy of 2D object detection has been dramatically improved, even beyond the human eyes detection ability. However, there is still a limitation of 2D object detection for the applications of Intelligent Driving. A safe and reliable self-driving car needs to detect a 3D model of the around objects so that an intelligent driving car has a perception ability to real driving situations. This paper systematically surveys the development of 3D object detection methods applied to intelligent driving technology. This paper also analyzes the shortcomings of the existing 3D detection algorithms and the future development directions of 3D detection algorithms on intelligent driving.
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http://dx.doi.org/10.1007/s10015-021-00711-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8559424PMC
November 2021

Emerging Severe Acute Respiratory Syndrome Coronavirus 2 Mutation Hotspots Associated With Clinical Outcomes and Transmission.

Front Microbiol 2021 18;12:753823. Epub 2021 Oct 18.

Guangxi Collaborative Innovation Center for Biomedicine, Guangxi Medical University, Nanning, China.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of the ongoing coronavirus disease 2019 (COVID-19) pandemic. Understanding the influence of mutations in the SARS-CoV-2 gene on clinical outcomes is critical for treatment and prevention. Here, we analyzed all high-coverage complete SARS-CoV-2 sequences from GISAID database from January 1, 2020, to January 1, 2021, to mine the mutation hotspots associated with clinical outcome and developed a model to predict the clinical outcome in different epidemic strains. Exploring the cause of mutation based on RNA-dependent RNA polymerase (RdRp) and RNA-editing enzyme, mutation was more likely to occur in severe and mild cases than in asymptomatic cases, especially A > G, C > T, and G > A mutations. The mutations associated with asymptomatic outcome were mainly in open reading frame 1ab (ORF1ab) and N genes; especially R6997P and V30L mutations occurred together and were correlated with asymptomatic outcome with high prevalence. D614G, Q57H, and S194L mutations were correlated with mild and severe outcome with high prevalence. Interestingly, the single-nucleotide variant (SNV) frequency was higher with high percentage of nt14408 mutation in RdRp in severe cases. The expression of ADAR and APOBEC was associated with clinical outcome. The model has shown that the asymptomatic percentage has increased over time, while there is high symptomatic percentage in Alpha, Beta, and Gamma. These findings suggest that mutation in the SARS-CoV-2 genome may have a direct association with clinical outcomes and pandemic. Our result and model are helpful to predict the prevalence of epidemic strains and to further study the mechanism of mutation causing severe disease.
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http://dx.doi.org/10.3389/fmicb.2021.753823DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8558435PMC
October 2021

Image reconstruction method for electrical capacitance tomography using adaptive simulated annealing algorithm.

Rev Sci Instrum 2021 Oct;92(10):105009

Department of Automation, North China Electric Power University, Baoding 071003, China.

Image reconstruction of electrical capacitance tomography (ECT) is an ill-posed inverse problem. The simulated annealing (SA) algorithm is suitable for the solution of the ECT inverse problem. However, selection of related parameters of the SA algorithm will influence the reconstruction performance of ECT. We present an ECT image reconstruction method based on the adaptive simulated annealing (ASA) algorithm. We adopt the bat algorithm in the new solution generation strategy of the ASA algorithm. Moreover, the definition of the energy function introduces the sparsity of the reconstructed image. As a result, an adaptive annealing strategy is proposed to select the appropriate annealing rate. We made reconstructed image comparisons among linear back-projection, Landweber iteration, SA, and ASA algorithms via simulation and static experiment. Results show that improved reconstructed images can be obtained using the ASA algorithm.
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http://dx.doi.org/10.1063/5.0059296DOI Listing
October 2021

MiRNA-494-3p Regulates Bupivacaine-Induced Neurotoxicity by the CDK6-PI3K/AKT Signaling.

Neurotox Res 2021 Oct 15. Epub 2021 Oct 15.

Department of Tumor Radiotherapy, Zhongshan Hospital Xiamen University, Siming District, 1854 Xinjing Garden, Luling Road, Xiamen, 361004, Fujian, China.

Bupivacaine (BUP) is a long-acting amide local anesthetic that may induce strong neurotoxicity and neurological complications. In this study, we elucidate the influence of microRNA-494-3p (miR-494-3p) in BUP-induced neurotoxicity in primary mouse hippocampal neuronal cells. In this study, primary hippocampal neurons were isolated from neonatal C57BL/6 mice. The isolated neurons were treated with various doses of BUP. MTT assay was conducted to analyze neuronal viability. Gene expression measurement was done by RT-qPCR. The impact of miR-494-3p in BUP-mediated neural injury was examined using TUNEL, flow cytometry, western blotting, and ROS activity detection. The regulatory relationship between miR-494-3p and cyclin-dependent kinases 4 and 6 (CDK6) was identified using a luciferase reporter assay. BUP treatment led to neurotoxicity and miR-494-3p upregulation in primary cultured hippocampal neurons. Functionally, miR-494-3p depletion alleviated neuronal apoptosis and oxidative damage induced by BUP. We verified that miR-494-3p targeted and negatively modulated CDK6. MiR-494-3p depletion also activated PI3K/AKT signaling by elevating CDK6 expression in BUP-treated neurons. Furthermore, CDK6 knockdown or PI3K/AKT inactivation attenuated the neuroprotective role of miR-494-3p depletion. Silencing miR-494-3p exerts neuroprotective function in hippocampal neuronal cells against BUP-induced injury by the CDK6-PI3K/AKT pathway.
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http://dx.doi.org/10.1007/s12640-021-00427-wDOI Listing
October 2021

The Association of Muscle Dysmorphia, Social Physique Anxiety, and Body Checking Behavior in Male College Students With Weight Exercise.

Front Psychol 2021 24;12:726032. Epub 2021 Sep 24.

Department of Student Affairs, Chengdu Sport University, Chengdu, China.

To investigate the association between muscle dysmorphia (MD), social physique anxiety, and body-checking behavior in male college students with weight exercise, and to reveal the association between them. A total of 492 male college students with weight exercise from 18 Fitness Clubs or bodybuilding centers in Chengdu, China, participated in this study. First, the social physique anxiety scores, body checking frequency, and weight exercise behavior (i.e., frequency, time, and intensity) in male college students with MD were significantly higher than those without MD; it indicated that the higher the exercise frequency they had, the longer the exercise time they cost, and the higher exercise intensity carried out, and the higher the social physique anxiety scores tended to be, the higher the frequency of body checking on "global muscles," "chest and shoulder muscles," "comparison with others" and "posture measurement" they did. Second, the mediating effect of the social physique anxiety on MD and body checking was established in the "MD → global muscle checking," "MD → chest and shoulder muscle checking," "MD → comparison with others," and "muscle dysmorphia → posture measurement." Male college students with MD not only have a higher social physique anxiety, but also a higher frequency of body-checking behavior than the ordinary individuals. Social physique anxiety is one of the important mediating factors to those with MD which affects the body-checking behavior.
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http://dx.doi.org/10.3389/fpsyg.2021.726032DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497756PMC
September 2021

Enhancing Li-ion conduction in composite polymer electrolytes using LiLaTiO nanotubes.

Chem Commun (Camb) 2021 Oct 21;57(84):11068-11071. Epub 2021 Oct 21.

Institute of Molecular Plus, Tianjin University, 92 Weijin Road, Tianjin 300072, P. R. China.

Here, a poly(vinylidene fluoride) (PVDF)-based composite polymer electrolyte (CPE) with a unique LiLaTiO (LLTO) nanotube filler, which shows a high Li-ion conductivity, is reported. Compared with LLTO nanoparticles (NPs) and nanowires (NWs), the LLTO nanotube fillers increase the interfacial area between PVDF and the LLTO filler, leading to the simple transportation of Li-ions through these interfacial pathways. In addition, the Li plating and stripping cycling performance of the CPEs is improved to 205 h at 0.1 mA cm, and the performance of the Li|CPEs|LiFePO cells also achieves a discharge capacity of 120 mA h g after 100 cycles at 0.5C at room temperature. These results demonstrate an effective interfacial strategy for the design of high Li-ion conduction CPEs for all solid-state batteries.
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http://dx.doi.org/10.1039/d1cc04220cDOI Listing
October 2021

Over-Expression of the Cell-Cycle Gene Promotes Cell Proliferation and the Formation of Normal Cotyledonary Embryos during Somatic Embryogenesis.

Genes (Basel) 2021 Sep 17;12(9). Epub 2021 Sep 17.

State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.

Somatic embryogenesis is an effective tool for the production of forest tree seedlings with desirable characteristics; however, the low initiation frequency and productivity of high-quality mature somatic embryos are still limiting factors for (Japanese larch). Here, we analyzed the expression pattern of   () during somatic embryogenesis in and its relationship with the cell proliferation rate. We also analyzed the effect of over-expression on somatic embryo quality. The results revealed a positive correlation between expression and the cell proliferation rate during the proliferation stage. After over-expression, the proliferation rate of cultures increased, and the number of somatic embryos in transgenic cultures was 2.69 times that in non-transformed cultures. Notably, the number of normal cotyledonary embryos in transgenic cultures was 3 times that in non-transformed cultures, indicating that not only increases the proliferation of cultures and the number of somatic embryos but also improves the quality of somatic embryos. These results provide insight into the regulatory mechanisms of somatic embryogenesis as well as new breeding material.
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http://dx.doi.org/10.3390/genes12091435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8468589PMC
September 2021

Lipidomic Alterations and PPAR Activation Induced by Resveratrol Lead to Reduction in Lesion Size in Endometriosis Models.

Oxid Med Cell Longev 2021 11;2021:9979953. Epub 2021 Sep 11.

The Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, China.

Endometriosis is an estrogen-dependent chronic inflammatory disease that affects approximately 10% of women of reproductive age and up to 50% of women with infertility. The heterogeneity of the disease makes accurate diagnosis and treatment a clinical challenge. In this study, we generated two models of endometriosis: the first in rats and the second using human ectopic endometrial stromal cells (HEcESCs) derived from the lesion tissues of endometriosis patients. We then applied resveratrol to assess its therapeutic potential. Resveratrol intervention had significant efficacy to attenuate lesion size and to rectify aberrant lipid profiles of model rats. Lipidomic analysis revealed significant lipidomic alterations, including notable increases of sphingolipids and decreases of both glycerolipids and most phospholipids. Upon resveratrol application, both proliferation capacity and invasiveness parameters decreased, and the early apoptosis proportion increased for HEcESCs. The activation of PPAR was also noted as a factor potentially contributing to recovery from endometriosis in both models. Our study provides valuable insight into the mechanisms of resveratrol in endometriosis and therefore strengthens the potential for optimizing resveratrol treatment for this disease.
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http://dx.doi.org/10.1155/2021/9979953DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452402PMC
September 2021

Adsorption of ammonia nitrogen and phenol onto the lignite surface: An experimental and molecular dynamics simulation study.

J Hazard Mater 2021 08 28;416:125966. Epub 2021 Apr 28.

School of Chemical and Environmental Engineering, China University of Mining & Technology (Beijing), Beijing 100083, China. Electronic address:

Ammonia nitrogen and phenol are typical inorganic and organic pollutants in the coal chemical wastewater, respectively. In this study, the adsorption characteristics of ammonia nitrogen and phenol on lignite were investigated through experimental and molecular dynamics simulations. The results show that the adsorption of ammonia nitrogen was carried out via ion exchange, which was significantly faster than the adsorption of phenol driven by the π-π interaction. In the binary adsorption, the surface electronegativity of lignite decreased with the adsorption of ammonia nitrogen thereby promoting the adsorption of phenol. However, the extent of ammonia nitrogen adsorption was slightly reduced in the presence of phenol. Molecular dynamics simulation results indicated that the adsorption of phenol molecules on the lignite surface was closer than that of ammonium ion. The addition of ammonium ions could enhance the adsorption of phenol molecules on the lignite surface. The simulation results were well consistent with the experimental findings. This study indicates lignite has a promising potential in coal chemical wastewater adsorption pretreatment.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125966DOI Listing
August 2021

Exosome-Derived Long Non-Coding RNAs as Non-Invasive Biomarkers of Bladder Cancer.

Front Oncol 2021 19;11:719863. Epub 2021 Aug 19.

Department of Urology, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, China.

Objective: As a result of the inconsistency between reports, a meta-analysis was designed to appraise the clinical implications of long non-coding RNAs (lncRNAs) in exosomes for the diagnosis of bladder cancer.

Methods: The PubMed, EMBASE, and Cochrane library databases were searched to identify the relevant literature on lncRNAs in exosomes for bladder cancer diagnosis from database inception to May 2021. The literature was screened according to the inclusion and exclusion criteria, and the Quality Assessment of Diagnostic Accuracy Studies-2 entry tool was applied to evaluate the quality of the literature, and the sources of heterogeneity were explored using meta-regression and subgroup analysis. Stata 14.0 and RevMan 5.3 software were used for statistical analysis.

Results: A total of 23 studies described in 10 articles were included, with a total of 1883 patients with bladder cancer and 1721 patients in the non-cancerous control group. The exosome-derived lncRNAs performed better in the diagnosis of bladder cancer with a pooled sensitivity of 0.74 (95% CI, 0.69-0.77), specificity of 0.76 (95% CI, 0.72-0.80), and area under the curve of 0.83. The heterogeneity between studies was partly as a result of differences in specimen type, number of lncRNAs, lncRNA expression form, and reference gene type. Subgroup analysis showed that the detection efficacy based on the combination of multiple lncRNAs (0.86, 95% CI, 0.82-0.88) was higher than that based on a single lncRNA (0.81, 95% CI, 0.78-0.85), and exosomal lncRNAs with blood as the detection sample had a high diagnostic efficacy (0.86, 95% CI, 0.82-0.86).

Conclusions: Exosome-derived lncRNAs hold great promise as non-invasive diagnostic biomarkers of bladder cancer. However, their clinical value needs to be examined in further comprehensive prospective studies.
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http://dx.doi.org/10.3389/fonc.2021.719863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417445PMC
August 2021

Structural Evolution of Cu/ZnO Catalysts during Water-Gas Shift Reaction: An Transmission Electron Microscopy Study.

ACS Appl Mater Interfaces 2021 Sep 24;13(35):41707-41714. Epub 2021 Aug 24.

Institute of Molecular Plus, Tianjin University, 92 Weijin Road, Tianjin 300072, P. R. China.

Supported metal catalysts experience significant structural evolution during the activation process and reaction conditions, which is critical to achieve a desired active surface and interface enabling efficient catalytic processes. However, such dynamic structural information and related mechanistic understandings remain largely elusive owing to the limitation of real-time capturing dynamic information under reaction conditions. Here, using environment transmission electron microscopy, we demonstrate the atomic-scale structural evolution of the model Cu/ZnO catalyst under relevant water-gas shift reaction (WGSR) conditions. Under a CO gas environment, Cu nanoparticles decompose into smaller Cu species and redistribute on ZnO supports with either the crystalline CuO or amorphous CuO phase due to a strong CO-Cu interaction. In addition, we visualize various metal-support interactions between Cu and ZnO under reaction conditions, e.g., ZnO clusters precipitating on Cu nanoparticles, which are critical to understand active sites of Cu/ZnO as catalysts for WGSR. These atomic-scale observations highlight the dynamic interplays between Cu and ZnO that can be extended to other supported metal catalysts.
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http://dx.doi.org/10.1021/acsami.1c11839DOI Listing
September 2021

Potency of miR-144-3p in promoting abdominal aortic aneurysm progression in mice correlates with apoptosis of smooth muscle cells.

Vascul Pharmacol 2021 Aug 5:106901. Epub 2021 Aug 5.

Department of Vascular Surgery, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610075, PR China. Electronic address:

Abdominal aortic aneurysm (AAA), a life-threatening disease, is commonly diagnosed among people with risk factors, including increasing age, male gender, and smoking. The apoptosis of smooth muscle cells (SMCs) has been reported to disrupt the vascular structural integrity, which causes AAA. Thus, we sought to characterize the potential role of microRNA (miR)-144-3p in SMC apoptosis, and to outline the molecular mechanisms involved in this pathway. We collected pathological abdominal aortic tissues and adjacent normal aortic biopsy specimens from 18 patients undergoing AAA repair surgery. The relationship between miR-144-3p expression and SMC proliferation was assessed by transfecting mimic/inhibitor of miR-144-3p in human aortic smooth muscle cells (HASMCs). Anti-growth effect of miR-144-3p and related genes was evaluated in a murine AAA model. Dual luciferase reporter gene assay was adopted to validate the targeting relationship between miR-144-3p and enhancer of zeste homolog 2 (EZH2), and the enrichment of EZH2 in the p21 promoter region was determined by chromatin immunoprecipitation assay. MiR-144-3p was highly expressed in AAA tissues. Enhanced miR-144-3p diminished SMC proliferation by binding to the EZH2 3'-untranslated region and thereby inhibiting EZH2 expression. In addition, EZH2 was highly enriched in the promoter region of p21, and knockdown of p21 expression could rescue the effect of miR-144-3p on SMC proliferation and apoptosis. miR-144-3p serves as a promoter for the apoptosis of SMCs, which contributes to the occurrence and progression of AAA. This observation will serve as the basis for further investigations into potential p21-based therapies for AAA treatment.
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http://dx.doi.org/10.1016/j.vph.2021.106901DOI Listing
August 2021

Effects of virtual reality cognitive training in individuals with mild cognitive impairment: A systematic review and meta-analysis.

Int J Geriatr Psychiatry 2021 12 7;36(12):1829-1847. Epub 2021 Aug 7.

School of Nursing, Sun Yat-sen University, Guangzhou, China.

Background: Virtual reality (VR) is used to improve specific health needs by combining multiple technologies; it is increasingly being used in the medical field, showing satisfactory effects, especially in the management of chronic disease. The aim of this study was to assess the effects of VR cognitive training for individuals with mild cognitive impairment (MCI).

Methods: Peer-reviewed articles were searched from the PubMed, Embase, Web of Science, the Cochrane Library, Science Direct, and EBSCOhost databases, as well as CNKI, Sinomed, Vip. and Wan Fang, through 23 May 2021. We only included randomized controlled trials (RCTs) enrolling participants with MCI.

Results: Seventeen RCTs were included, with a total of 744 participants. Evidence of moderate quality showed that VR cognitive training significantly enhanced MCI patients' global cognitive function, as measured by the Montreal Cognitive Assessment (standardized mean difference [SMD] = 0.42; 95% confidence interval [CI], 0.04-0.79; p = 0.03) and executive function, as measured by trail making test A (SMD = -0.58; 95% CI, -0.80 to -0.35; p < 0.001). The meta-analysis indicated that the effects of VR cognitive training on delayed memory, immediate memory, attention and instrumental activities of daily living were not statistically significant (p > 0.05).

Conclusion: The available data showed that VR cognitive training might be beneficial for improving global cognitive function and executive function in individuals with MCI, although the effects were short term.
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http://dx.doi.org/10.1002/gps.5603DOI Listing
December 2021

Atomic-Scale Interfacial Phase Transformation Governed Cu Oxidation in Water Vapor.

J Phys Chem Lett 2021 Jul 20;12(29):6996-7001. Epub 2021 Jul 20.

Institute of Molecular Plus, Tianjin University, 92 Weijin Road, Tianjin 300072, China.

Metal oxidation initiates from surface adsorption to subsurface and bulk reaction through continuous interfacial phase transformation from metals to oxides. How the initial interfacial process affects the whole process of metal oxidation remains largely elusive because of the lack of direct observation of the evolving interface. Here, through atomic-scale environmental TEM observations of Cu surface reaction in water vapor, we demonstrate that the interfacial strain between the substrate and growing oxide is coupled into the continuing chemical reaction that determines the reaction kinetics. Atomic imaging of the reaction process in real time reveals that the growing oxides could temporarily possess a disordered CuO phase to lower its interfacial strain with Cu substrate and can transform to a crystalline CuO phase later. This flexibility of the oxide phase results from the strong chemomechanical coupling during the interfacial phase transformation, which enhances the oxide penetration into the metal under water vapor.
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http://dx.doi.org/10.1021/acs.jpclett.1c02008DOI Listing
July 2021

RNF43 is a Novel Tumor Suppressor and Prognostic Indicator in Clear Cell Renal Cell Carcinoma.

Oncol Res 2021 Jul 2. Epub 2021 Jul 2.

Department of Urology, the Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, 29 Xinglong Road. Changzhou, Jiangsu 213000, China.

Identifying prognostic indicators of clear cell renal cell carcinoma (ccRCC) and elucidating the mechanisms underlying ccRCC progression are crucial for improving ccRCC patient prognosis. This study investigated the clinical significance and biological role of Ring finger protein 43 ( RNF43) in ccRCC. Two independent cohorts of patients with ccRCC were employed to determine the prognostic significance of RNF43 by immunohistochemistry and statistical analyses. In vitro and in vivo experiments, RNA-seq, and other techniques were used to determine the biological role of RNF43 in ccRCC and related molecular mechanisms. RNF43 expression was commonly decreased in ccRCC specimens, and low expression of RNF43 indicated a higher TNM stage, SSIGN score, and WHO/ISUP grade and short survival in patients with ccRCC. Additionally, RNF43 overexpression suppressed the proliferation, migration, and targeted drug resistance of ccRCC cells, while the knockdown of RNF43 enhanced these characteristics of ccRCC. RNF43 knockdown activated YAP signaling by decreasing YAP phosphorylation by p-LATS1/2 and increasing the transcription and nuclear distribution of YAP. By contrast, RNF43 overexpression showed the opposite effects. Decreasing YAP abolished the effect of RNF43 knockdown in promoting the malignant features of ccRCC. Additionally, restoring RNF43 expression suppressed the resistance of the targeted drug pazopanib in in vivo orthotopic ccRCC. Furthermore, combining the expression of RNF43 andYAP with TNM stage or the SSIGN score exhibited greater accuracy than any of these indicators alone in assessing the postoperative prognosis of ccRCC patients. In summary, our study identified a novel tumor suppressor, RNF43, which is also a prognostic indicator and potential target for ccRCC potential target for ccRCC.
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http://dx.doi.org/10.3727/096504021X16252201000833DOI Listing
July 2021

Examination of the Transcriptional Response to Overexpression in (Lamb.) Carr.

Biology (Basel) 2021 Jun 23;10(7). Epub 2021 Jun 23.

State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China.

The study of somatic embryogenesis can provide insight into early plant development. We previously obtained -overexpressing embryonic cell lines of (Lamb.) Carr. To further elucidate the molecular mechanisms associated with in this species, the transcriptional profiles of wild-type (WT) and three -overexpressing transgenic cell lines were subjected to RNA sequencing using the Illumina NovaSeq 6000 system. In total, 203,256 unigenes were generated using Trinity de novo assembly, and 2467 differentially expressed genes were obtained by comparing transgenic and WT lines. In addition, we analyzed the cleaved degree of target genes in different transgenic cell lines by detecting the expression pattern of , and their cleaved degree in transgenic cell lines was higher than that in WT. The downstream genes of were identified using Pearson correlation coefficients. Yeast one-hybrid and dual-luciferase report assays revealed that the transcription factors LaHDZ31-34 could bind to the promoters of , , , and . This is the first report of gene expression changes caused by overexpression in Japanese larch. These findings lay a foundation for future studies on the regulatory mechanism of miR166.
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http://dx.doi.org/10.3390/biology10070576DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8301127PMC
June 2021

Remediation of GenX from water by amidoxime surface-functionalized electrospun polyacrylonitrile nanofibrous adsorbent.

Chemosphere 2021 Nov 16;283:131235. Epub 2021 Jun 16.

Department of Nanoengineering, Joint School of Nanoscience and Nanoengineering, North Carolina A&T State University, Greensboro, NC, 27401, USA. Electronic address:

As a short-chain PFAS, GenX has gained increasing attention in recent years as a hazardous and emerging contaminant in water bodies. However, there is only limited research outcome up to date to address GenX remediation from water. In this research, we investigated amidoxime surface-functionalized PAN nanofibrous material from electrospinning as adsorbent to remove GenX from water. The nanofibrous adsorbent from 10 min treatment of electrospun PAN nanofibrous material in hydroxylamine (ASFPAN10) realized 35% GenX removal from a 100 mg/L aqueous solution at pH = 4 and 0.24 g/L loading after a simple one-time filtration with a GenX removal capacity of ~0.6 mmol/g. The mechanism study indicated that the GenX adsorption on PAN nanofibrous adsorbent could be mainly ascribed to hydrophobic interaction and dipole-dipole interaction between CN and C-F while the GenX adsorption on ASFPAN10 nanofibrous adsorbent could be mainly attributed to coulomb force between positive-charged CN(OH)-H from ASFPAN10 and negative-charged COO from GenX. Compared to that of PAN, the more hydrophilic surface of ASFPAN10 facilitated water access to the nanofibrous adsorbent surface and also contributed to the higher GenX removal efficiency. For the first time, this research pointed out a direction to use common economic materials for effective remediation of short-chain PFAS from water bodies especially at relatively high PFAS centration.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131235DOI Listing
November 2021

Lemon exosome-like nanoparticles enhance stress survival of gut bacteria by RNase P-mediated specific tRNA decay.

iScience 2021 Jun 5;24(6):102511. Epub 2021 May 5.

Robley Rex Veterans Affairs Medical Center, Louisville, KY 40206, USA.

Diet and bile play critical roles in shaping gut microbiota, but the molecular mechanism underlying interplay with intestinal microbiota is unclear. Here, we showed that lemon-derived exosome-like nanoparticles (LELNs) enhance lactobacilli toleration to bile. To decipher the mechanism, we used GG (LGG) as proof of concept to show that LELNs enhance LGG bile resistance via limiting production of Msp1 and Msp3, resulting in decrease of bile accessibility to cell membrane. Furthermore, we found that decline of Msps protein levels was regulated through specific tRNA and tRNA decay. We identified RNase P, an essential housekeeping endonuclease, being responsible for LELNs-induced tRNA and tRNA decay. We further identified galacturonic acid-enriched pectin-type polysaccharide as the active factor in LELNs to increase bile resistance and downregulate tRNA and tRNA level in the LGG. Our study demonstrates a tRNA-based gene expression regulation mechanism among lactobacilli to increase bile resistance.
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http://dx.doi.org/10.1016/j.isci.2021.102511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188359PMC
June 2021

USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.

Sci Total Environ 2021 Aug 9;783:146898. Epub 2021 Apr 9.

Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210009, China.

Di-n-butylphthalate (DBP) has been listed as an environmental priority pollutant in China due to its distinct biotoxicity. Epidemiological studies have shown that exposure to DBP is closely related to a series of congenital and acquired defects in the male reproductive system. The oxidative stress injury caused by DBP plays an important role in these defects. Previous studies have demonstrated that the Keap1/Nrf2 antioxidative pathway plays a protective role in DBP-induced oxidative stress injury. However, the further molecular regulation mechanism of the activation of Nrf2 pathway remains unclear. Here, we demonstrate that DBP caused testicular oxidative stress injury and Nrf2 pathway was activated in response to the injury in vivo and in vitro. Moreover, we validated that reduced level of USP15 attenuates DBP-induced oxidative stress injury through restraining the ubiquitylation and degradation of Nrf2. Notably, USP15 is confirmed as a target of miR-135b-5p and miR-135b-5p mediated inhibition of USP15 is involved in the DBP-induced oxidative stress injury. Collectively, these findings indicated that decreased level of USP15 functions a significant protective effect on the oxidative stress injury of testis caused by DBP via regulating the Keap1/Nrf2 signaling pathway.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146898DOI Listing
August 2021

Isomeric Dibenzoheptazethrenes for Air-Stable Organic Field-Effect Transistors.

Angew Chem Int Ed Engl 2021 Jul 15;60(29):16230-16236. Epub 2021 Jun 15.

Institute of Molecular Plus, Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Tianjin university, 92 Weijin Road, Tianjin, 300072, China.

Singlet diradicaloids hold great potential as semiconductors for organic field-effect transistors (OFETs). However, their relative low material and device stabilities impede the practical applications. Here, to achieve balanced stability and performance, two isomeric dibenzoheptazethrene derivatives with singlet diradical character were synthesized in a concise manner. Benefitting from the aromatic stabilization, both compounds display a small diradical character and large singlet-triplet gap, as corroborated by variable-temperature electron paramagnetic resonance spectra, single-crystal analysis, and theoretical calculations. OFET devices based on single crystals showed a high hole mobility of 0.15 cm  V  s , which is the highest for zethrene-based semiconductors. Both isomers exhibited remarkable material stability in air-saturated solutions as well as excellent bias-stress and storage stability in device under ambient air.
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http://dx.doi.org/10.1002/anie.202105872DOI Listing
July 2021

Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12.

Mol Ther 2021 08 11;29(8):2424-2440. Epub 2021 May 11.

Robley Rex Veterans Affairs Medical Center, Louisville, KY 40206, USA; Department of Microbiology & Immunology, University of Louisville, Louisville, KY 40202, USA; James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA. Electronic address:

Lung inflammation is a hallmark of coronavirus disease 2019 (COVID-19). In this study, we show that mice develop inflamed lung tissue after being administered exosomes released from the lung epithelial cells exposed to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Nsp12 and Nsp13 (exosomes). Mechanistically, we show that exosomes are taken up by lung macrophages, leading to activation of nuclear factor κB (NF-κB) and the subsequent induction of an array of inflammatory cytokines. Induction of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β from exosomes-activated lung macrophages contributes to inducing apoptosis in lung epithelial cells. Induction of exosomes-mediated lung inflammation was abolished with ginger exosome-like nanoparticle (GELN) microRNA (miRNA aly-miR396a-5p. The role of GELNs in inhibition of the SARS-CoV-2-induced cytopathic effect (CPE) was further demonstrated via GELN aly-miR396a-5p- and rlcv-miR-rL1-28-3p-mediated inhibition of expression of Nsp12 and spike genes, respectively. Taken together, our results reveal exosomes as potentially important contributors to the development of lung inflammation, and GELNs are a potential therapeutic agent to treat COVID-19.
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http://dx.doi.org/10.1016/j.ymthe.2021.05.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110335PMC
August 2021

A Cross-Sectional Survey of Family Care Behaviors for Children with Upper Respiratory Tract Infections in China: Are There Opportunities for Improvement?

J Pediatr Nurs 2021 Sep-Oct;60:146-153. Epub 2021 May 6.

School of Nursing, Sun Yat-sen University, China. Electronic address:

Objective: To describe family care behaviors for children with upper respiratory tract infections (URTIs) and explore related factors.

Design And Methods: Parents of children with URTIs were included in this cross-sectional study. Family care behaviors, disease-related knowledge, and parental self-efficacy were evaluated with validated measures.

Results: Among the 419 participants, 73.80% recognized diseases based on their children's abnormal presentation. Self-medication was the main home care measure (36.28% used only self-medication; 27.92% used both self-medication and physical cooling methods), and 36.5% received suggestions from medical professionals. All the participants took their children to the hospital, and 28.20% did so two or three times. The proportions of visits to level II or III hospitals were 49.64% and 83.87% for first and third hospital visits, respectively. Parents who had less disease knowledge and assessed children' diseases as more serious took their children to the hospital more often (p < 0.05); those whose nearest medical institution was a community health center were more likely to visit such centers (p < 0.001).

Conclusions: Most of the parents recognized symptoms of URTIs and provided home care but lacked enough knowledge and professional support to take reasonable measures. Hospital visits were their primary choice.

Practical Implications: Family care behaviors for children with URTIs could be improved through health education, and an internet nursing service or family doctor system is suggested. A hierarchical medical system is necessary to reduce hospital visits, as are more community health centers with pediatric services.
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http://dx.doi.org/10.1016/j.pedn.2021.04.033DOI Listing
October 2021

Letter to the Editor Regarding "Microvascular Decompression versus Stereotactic Radiosurgery for Trigeminal Neuralgia: A Single-Institution Experience".

World Neurosurg 2021 05;149:279

Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, China. Electronic address:

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http://dx.doi.org/10.1016/j.wneu.2021.01.074DOI Listing
May 2021

Role of Hakai in mA modification pathway in Drosophila.

Nat Commun 2021 04 12;12(1):2159. Epub 2021 Apr 12.

State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China.

N6-methyladenosine (mA), the most abundant internal modification in eukaryotic mRNA, is installed by a multi-component writer complex; however, the exact roles of each component remain poorly understood. Here we show that a potential E3 ubiquitin ligase Hakai colocalizes and interacts with other mA writer components, and Hakai mutants exhibit typical mA pathway defects in Drosophila, such as lowered mA levels in mRNA, aberrant Sxl alternative splicing, wing and behavior defects. Hakai, Vir, Fl(2)d and Flacc form a stable complex, and disruption of either Hakai, Vir or Fl(2)d led to the degradation of the other three components. Furthermore, MeRIP-seq indicates that the effective mA modification is mostly distributed in 5' UTRs in Drosophila, in contrast to the mammalian system. Interestingly, we demonstrate that mA modification is deposited onto the Sxl mRNA in a sex-specific fashion, which depends on the mA writer. Together, our work not only advances the understanding of mechanism and regulation of the mA writer complex, but also provides insights into how Sxl cooperate with the mA pathway to control its own splicing.
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http://dx.doi.org/10.1038/s41467-021-22424-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8041851PMC
April 2021

Triple-helix polysaccharides: Formation mechanisms and analytical methods.

Carbohydr Polym 2021 Jun 20;262:117962. Epub 2021 Mar 20.

Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON, N1G 5C9, Canada.

Triple helix conformation of polysaccharides is generally believed to be associated with their biological activities. However, the formation mechanisms and analytical methods for the triple helix polysaccharides, to our best knowledge, have not been systematically investigated. This article reviews specifically the literature on the formation and characterization of triple-helix polysaccharides. The formation mechanisms and related structural-conformation-bioactivity relationships are discussed; various analytical methods for characterizing triple-helix polysaccharides are summarized. This review devotes to a better understanding of the formation of polysaccharides based triple-helix structure and related analytical methods. These could provide some insights and inspirations for their applications in both food and pharmaceutical industries.
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http://dx.doi.org/10.1016/j.carbpol.2021.117962DOI Listing
June 2021

Clinical effect of Brisson operation modified by Y-shaped incision for treatment of concealed penis in adolescents.

J Int Med Res 2021 Apr;49(4):3000605211005951

Department of Urology, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu, China.

Objective: To analyze the clinical effect of the Brisson operation modified by a Y-shaped incision in treating adolescent concealed penis.

Methods: We retrospectively analyzed clinical data of 27 adolescents with a concealed penis treated with the Brisson operation modified by a Y-shaped incision in our hospital from January 2017 to March 2020.

Results: The operation went smoothly in all 27 patients. Postoperative foreskin edema occurred in 12 patients and spontaneously resolved within 1 month postoperatively. Two patients developed postoperative retropubic infection. After administering antibiotics and symptomatic treatment, both patients' conditions improved within 1 week. All operations obtained satisfactory results. Postoperatively, when the penis was in a non-erect state, it was clearly exposed without retraction or concealment; thus, demonstrating good surgical results. The prepuce was distributed naturally without obvious accumulation of redundant preputial tissue. The penile scar resembled that after circumcision. The postoperative follow-up period was 6 months, during which no patients developed recurrence.

Conclusion: The Brisson operation modified by a Y-shaped incision is effective for treating a concealed penis in adolescent patients. This technique may relieve the pathological abnormalities and retain the penile skin's integrity to the greatest extent with minimal scarring and a satisfactory appearance.
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http://dx.doi.org/10.1177/03000605211005951DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033467PMC
April 2021

ATF3 Suppresses Growth and Metastasis of Clear Cell Renal Cell Carcinoma by Deactivating EGFR/AKT/GSK3β/β-Catenin Signaling Pathway.

Front Cell Dev Biol 2021 19;9:618987. Epub 2021 Mar 19.

Department of General Surgery, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, China.

Background: Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant cancers in East Asia, with high incidence and mortality. Accumulating evidence has shown that ATF3 is associated with tumor progression.

Methods: Using qPCR, the expression of ATF3 was detected in 93 patients with ccRCC, including 24 paired normal and tumor tissues, which were used to further compare ATF3 expression through western blotting and immunohistochemistry. Lentivirus was used for the overexpression or knockdown of ATF3, and the consequent alteration in function was analyzed through CCK8 assay, colony formation assay, wound healing assay, invasion assay, and flow cytometry. The potential mechanism affected by ATF3 was analyzed through gene set enrichment analysis (GSEA) and verified using western blotting, invasion assay, or immunofluorescence staining. Furthermore, a xenograft mouse model was used to assess the function of ATF3 .

Results: ATF3 expression was significantly decreased in ccRCC compared to that in adjacent normal tissues. Through gain- and loss-of-function experiments performed in an assay, we found that ATF3 could regulate ccRCC cell proliferation, cycle progression, migration, and invasion. In the study, the xenograft mouse model revealed that ATF3 overexpression can inhibit the growth of ccRCC. Moreover, the mechanism analysis showed that suppression of ATF3 could lead to an increase the expression of β-catenin and promote β-catenin transfer to the nucleus, and might be affected by EGFR/AKT/GSK3β signaling.

Conclusion: ATF3 could be utilized as an independent protective factor to inhibit the progression of ccRCC. Potential treatment strategies for ccRCC include targeting the ATF3/EGFR/AKT/GSK3β/β-catenin signaling pathway.
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http://dx.doi.org/10.3389/fcell.2021.618987DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8017234PMC
March 2021
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