Publications by authors named "Lichun Zhang"

100 Publications

Complete genome analysis of the newly isolated Shigella sonnei phage vB_SsoM_Z31.

Arch Virol 2021 Jun 11. Epub 2021 Jun 11.

Dalian SEM Bio-Engineering Technology Co. Ltd, Dalian, 116620, China.

This work describes the characterization and genome annotation of the newly isolated lytic phage vB_SsoM_Z31 (referred to as Z31), isolated from wastewater samples collected in Dalian, China. Transmission electron microscopy revealed that phage Z31 belongs to the family Myoviridae, order Caudovirales. This phage specifically infects Shigella sonnei, Shigella dysenteriae, and Escherichia coli. The genome of the phage Z31 is an 89,355-bp-long dsDNA molecule with a G+C content of 38.87%. It was predicted to contain 133 ORFs and encode 24 tRNAs. No homologs of virulence factor genes or antimicrobial resistance genes were found in this phage. Based on the results of nucleotide sequence alignment and phylogenetic analysis, phage Z31 was assigned to the genus Felixounavirus, subfamily Ounavirinae.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00705-021-05121-yDOI Listing
June 2021

Translation, ribosome biogenesis, and oxidative damage caused by chlorpyrifos exposure to common carp (Cyprinus carpio L.) liver: application of combined RNA-seq with sRNA-seq in risk evaluation of environmental toxicant chlorpyrifos.

Environ Sci Pollut Res Int 2021 May 28. Epub 2021 May 28.

College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, No. 501 Zhongkai Road, Haizhu District, Guangzhou, China.

Pesticide chlorpyrifos (CPF) is a widespread environmental pollutant gaining attention as it is highly injurious to aquatic life. Although the toxicity of CPF is well characterized, but the mechanism of toxic response especially, the hepatotoxicity remained unclear. In this study, we performed integrated analysis, including micro-RNA (miRNA) and small RNA (sRNA) to analyze CPF exposure responding genes and enrichment pathways. A total of 23,742 expressed genes were detected and out of these expression levels of 1746 were changed significantly. Majority of them participated in protein biosynthesis, nucleotide binding, and oxidation-reduction activities. In extensive analysis of micro-RNA (miRNA) expression profiles by comparing CPF treated carp with control, we identified 214 novel miRNAs with CPM > 5 in at least one sample. The miRNAs have the same change in direction compared with overlapped mRNA pairs in upregulated genes, suggesting potential positive correlation. As a whole, we detected many differently expressed genes (DEGs) and miRNAs, which may be used as the biomarkers for the detection of CPF pollution in water and aquatic product safety. However, their functions are required to be deeply analyzed, especially more samples or time pointed data are needed to illustrate their concrete mechanism.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11356-021-14491-4DOI Listing
May 2021

Identification and validation of miRNA reference genes in poplar under pathogen stress.

Mol Biol Rep 2021 Apr 4;48(4):3357-3366. Epub 2021 May 4.

Beijing Advanced Innovation Center for Tree Breeding By Molecular Design, College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, China.

Quantitative real time polymerase chain reaction (qRT-PCR) is a common method to analyze gene expression. Due to differences in RNA quantity, quality, and reverse transcription efficiency between qRT-PCR samples, reference genes are used as internal standards to normalize gene expression. However, few universal genes, especially miRNAs, have been identified as reference so far. Therefore, it is essential to identify reference genes that can be used across various experimental conditions, stress treatments, or tissues. In this study, 14 microRNAs (miRNAs) and 5.8S rRNA were assessed for expression stability in poplar trees infected with canker pathogen. Using geNorm, NormFinder and Bestkeeper reference gene analysis programs, we found that miR156g and miR156a exhibited stable expression throughout the infection process. miR156g, miR156a and 5.8S rRNA were then tested as internal standards to measure the expression of miR1447 and miR171c, and the results were compared to small RNA sequencing (RNA-seq) data. We found that when miR156a and 5.8S rRNA were used as the reference gene, the expression of miR1447 and miR171c were consistent with the small RNA-seq expression profiles. Therefore, miR156a was the most stable miRNAs examined in this study, and could be used as a reference gene in poplar under canker pathogen stress, which should enable comprehensive comparisons of miRNAs expression and avoid the bias caused by different length between detected miRNAs and traditional reference genes. The present study has expanded the miRNA reference genes available for gene expression studies in trees under biotic stress.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11033-021-06369-yDOI Listing
April 2021

Ratiometric Cataluminescence Sensor of Amine Vapors for Discriminating Meat Spoilage.

Anal Chem 2021 05 22;93(17):6692-6697. Epub 2021 Apr 22.

Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.

The freshness of meat has always been the focus of attention from consumers and suppliers for health and economic reasons. Usually, amine vapors, as one of the main components of the gas produced in the process of meat spoilage, can be used to monitor meat spoilage. Here, a new ratiometric cataluminescence (CTL) sensor based on energy transfer was developed to identify amine vapors and monitor meat freshness. After Tb doping, amine vapors exhibit a dual-wavelength (490 and 555 nm) property of CTL signals when reacted on the surface of Tb-doped LaOCO, and the ratio of to () is a unique value for a given analyte within a wide range of concentrations. To illustrate the new sensor, 15 amine vapors were successfully identified using , including homologues and isomers. Besides, this sensor was used to monitor four meats, and the freshness of meats can be distinguished by cluster analysis successfully. Moreover, further discussion of energy-transfer phenomena and influence factors has facilitating effects on exploring the mechanism of energy transfer at the gas-solid interface.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.1c00034DOI Listing
May 2021

miRNA Mediated Regulation and Interaction between Plants and Pathogens.

Int J Mol Sci 2021 Mar 13;22(6). Epub 2021 Mar 13.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China.

Plants have evolved diverse molecular mechanisms that enable them to respond to a wide range of pathogens. It has become clear that microRNAs, a class of short single-stranded RNA molecules that regulate gene expression at the transcriptional or post-translational level, play a crucial role in coordinating plant-pathogen interactions. Specifically, miRNAs have been shown to be involved in the regulation of phytohormone signals, reactive oxygen species, and gene expression, thereby modulating the arms race between hosts and pathogens. Adding another level of complexity, it has recently been shown that specific lncRNAs (ceRNAs) can act as decoys that interact with and modulate the activity of miRNAs. Here we review recent findings regarding the roles of miRNA in plant defense, with a focus on the regulatory modes of miRNAs and their possible applications in breeding pathogen-resistance plants including crops and trees. Special emphasis is placed on discussing the role of miRNA in the arms race between hosts and pathogens, and the interaction between disease-related miRNAs and lncRNAs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms22062913DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999934PMC
March 2021

A Two-Photon Excited Near-Infrared Iridium(III) Complex for Multi-signal Detection and Multimodal Imaging of Hypochlorite.

Anal Chem 2021 03 3;93(10):4628-4634. Epub 2021 Mar 3.

Analytical & Testing Center, Sichuan University, Chengdu 610064, China.

Hypochlorite (ClO), as a type of reactive oxygen species (ROS), plays a crucial role in the process of oxidative stress and is closely related to many diseases. Thus, developing a method for detecting and imaging of ClO with high sensitivity and selectivity is of great significance. However, the applications of most luminescent probes are limited to the fact that the excitation and emission wavelengths of them are in the visible light region rather than in the near-infrared (NIR) region. Hence, an NIR iridium(III) complex () with two-photon excitation is designed for the detecting and imaging of ClO. In the presence of ClO, the luminescent intensity and lifetime of are remarkably enhanced. Interestingly, also exhibits excellent electrochemiluminescence (ECL) properties, and the ECL signal is significantly enhanced with the addition of ClO. What is more, is also suitable for the detection and analysis ClO by flow cytometry. Therefore, is developed to detect multiple signals and is successfully applied to detect exogenous and endogenous ClO in living cells with one-photon, two-photon, and phosphorescence lifetime image microscopy (PLIM). In addition, was successfully used for imaging of ClO in tissues and inflammatory mouse models. All of the above results indicate that is highly effective in detecting ClO in living systems.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.0c05460DOI Listing
March 2021

The Tn7-based genomic integration is dependent on an attTn7 box in the glms gene and is site-specific with monocopy in Ralstonia solanacearum species complex.

Mol Plant Microbe Interact 2021 Mar 3. Epub 2021 Mar 3.

The Ninth Peoples Hospital of Chongqing, Chongqing, China, Chongqing, China;

The Tn7-based genomic integration system enables direct insertion of foreign gene elements into chromosome downstream of glms in many bacteria species. The glms gene is greatly conserved in Ralstonia solanacearum species complex (RSSC), while its downstream regions are mostly different in the RSSC. Here, we provided genetic evidence to validate that this Tn7-integration is dependent on a conserved 30-bp motif in the glms, called attTn7 box, and artificial attTn7 boxes elsewhere are competent for the Tn7-integration, which is further confirmed to be simultaneous at downstream of both original and artificial attTn7 boxes using the PCR. With the whole genome re-sequencing on 500 Tn7-colonies, the Tn7-integration was confirmed to be site- specific at 25-bp downstream of glms with monocopy as chromosome of the RSSC. Characteristic of the monocopy in chromosome enables the Tn7-based complementation to fully restore phenotypes of mutants to those of parent strains that is advantageous than those based on plasmids with low-copy numbers. The Tn7-based genomic integration system provides a generally applicable and versatile genetic tool for studies of complementation, pathogenesis, overexpression, and in-vivo promoter activity assays with monocopy in the RSSC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1094/MPMI-11-20-0325-SCDOI Listing
March 2021

Novel Strategy for Engineering the [email protected] [email protected] Architecture and Its Applications in Cataluminescence Sensing.

ACS Appl Mater Interfaces 2021 Jan 5;13(2):3471-3480. Epub 2021 Jan 5.

Analytical & Testing Center, Sichuan University, Chengdu 610064, China.

Cataluminescence is an attractive oxydic luminescence on the gas-solid interface, and [email protected] [email protected] architectures show great potential for cataluminescence sensing due to their integrated synergistic effect from core and shell components. However, restricting the direct nucleation and growth of metal-organic frameworks (MOFs) on the topologically distinct surface of metal oxides is a great challenge, owing to the high interface energy from the topology mismatch. Herein, for the first time, a novel liquid-phase concentration-controlled nucleation strategy is exploited to induce the direct assembly of a ZIF-8 layer on the surface of CeO nanospheres without any sacrificial templates or further surface modifications. The results show that the construction of the [email protected] [email protected] architecture can be accomplished within 1 min under the mediation of boosted nucleation kinetics. Furthermore, the universality of this developed strategy is demonstrated by the encapsulation of other metal-oxide cores such as magnetic FeO and ZnCoO core particles with a ZIF-8 shell. Notably, compared to the pure CeO and ZIF-8, the obtained [email protected] nanocomposite exhibits enhanced analytical performance for the cataluminescence sensing of propanal, in which the shell acts as the major catalytic reaction center, while the core contributes to further improving the catalytic efficiency. The proposed facile synthesis strategy with excellent simplicity, rapidity, and universality brings new insights into the engineering of [email protected] advanced functional materials with mismatched topologies for catering to the diverse application demands.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c20799DOI Listing
January 2021

Incus Dislocation and Traumatic Tympanic Membrane Perforation as a Complication of Middle Cranial Fossa Repair of Tegmen Dehiscence.

Ear Nose Throat J 2020 Aug 25:145561320950595. Epub 2020 Aug 25.

Department of Otorhinolaryngology, 14903Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/0145561320950595DOI Listing
August 2020

Combined signaling of NF-kappaB and IL-17 contributes to Mesenchymal stem cells-mediated protection for Paraquat-induced acute lung injury.

BMC Pulm Med 2020 Jul 17;20(1):195. Epub 2020 Jul 17.

Department of Emergency, Shengjing Affiliated Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang, 110004, Liaoning Province, China.

Background: Paraquat (PQ) is an herbicide widely used in the world. PQ can cause pulmonary toxicity and even acute lung injury. Treatment for PQ poisoning in a timely manner is still a challenge for clinicians. Mesenchymal stem cell (MSC) transplantation has hold potentials for the treatment of several lung diseases including PQ poisoning. The aim of this study is to examine the mechanisms mediated by MSC transplantation to protect PQ-induced lung injury.

Methods: Here we performed the whole genome sequencing and compared the genes and pathways in the lung that were altered by PQ or PQ together with MSC treatment.

Results: The comparison in transcriptome identified a combined mitigation in NF-kappaB signaling and IL-17 signaling in MSC transplanted samples.

Conclusion: This study not only reiterates the important role of NF-kappaB signaling and IL-17 signaling in the pathogenesis of PQ-induced toxicity, but also provides insight into a molecular basis of MSC administration for the treatment of PQ-induced toxicity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12890-020-01232-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367411PMC
July 2020

The effect of Acot2 overexpression or downregulation on the preadipocyte differentiation in Chinese Red Steppe cattle.

Adipocyte 2020 12;9(1):279-289

Branch of Animal Husbandry, Jilin Academy of Agricultural Science , Gongzhuling, China.

The quality and nutritional value of beef is closely linked to its content of intramuscular fat (IMF). The differentiation of preadipocytes and the deposition of lipid droplets in the adipocytes are the key to regulate the IMF content. The differentiation of adipocytes is regulated by a series of transcription factors and genes. Acyl-CoA thioesterase 2 (Acot2) hydrolyzes the acyl-coenzyme A (CoA) into free fatty acids and CoA and has the potential to maintain the free fatty acids and acyl CoA at the cellular level. In this work, we detected the expression of the gene during the adipocyte differentiation in Chinese Red Steppe cattle, and then interfered and overexpressed the Acot2 gene in the preadipocytes to explore its regulatory role in the adipocyte differentiation. The results showed that the expression and regulation of Acot2 mainly occurred at the later stage of the adipocyte differentiation. The interference with the Acot2 gene significantly inhibited the lipid droplets accumulation and triglyceride content, while its overexpression significantly promoted both of them. The results of this study show that the Acot2 gene is a positive regulator of the adipocyte differentiation and may become a new target to improve the beef quality.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/21623945.2020.1776553DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469445PMC
December 2020

Recent advances in methodologies and applications of cataluminescence sensing.

Luminescence 2020 Dec 22;35(8):1174-1184. Epub 2020 Jun 22.

Analytical & Testing Center, Sichuan University, Chengdu, Sichuan, China.

Cataluminescence (CTL), a kind of chemiluminescence emitted at the gas-solid interface during catalytic oxidation reactions, has been developed for many decades as a novel and promising gas sensing technique. In this review, we introduce the origin, basic principles, and mechanisms of CTL sensing systems and summarize the recent advances in CTL sensing, focusing on methodologies and extended applications such as in gas selective monitoring, recognition of complex mixture, evaluation for catalytic property and use in high-performance liquid chromatography, capillary electrophoresis and gas chromatography detectors. In addition, development prospects and some challenges facing CTL-based sensing are also discussed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/bio.3885DOI Listing
December 2020

Expression of Ralstonia solanacearum type III secretion system is dependent on a novel type 4 pili (T4P) assembly protein (TapV) but is T4P independent.

Mol Plant Pathol 2020 06 20;21(6):777-793. Epub 2020 Mar 20.

Research Institute of Molecular Genetics, Kochi University, Kochi, Japan.

Type IV pili (T4P) are virulence factors in various pathogenic bacteria of animals and plants that play important roles in twitching motility, swimming motility, biofilm formation, and adhesion to host cells. Here, we genetically characterized functional roles of a putative T4P assembly protein TapV (Rsc1986 in reference strain GMI1000) and its homologue Rsp0189, which shares 58% amino acid identity with TapV, in Ralstonia solanacearum. Deletion of tapV, but not rsp0189, resulted in significantly impaired twitching motility, swimming motility, and adhesion to tomato roots, which are consistent as phenotypes of the pilA mutant (a known R. solanacearum T4P-deficient mutant). However, unlike the pilA mutant, the tapV mutant produced more biofilm than the wild-type strain. Our gene expression studies revealed that TapV, but not Rsp0189, is important for expression of a type III secretion system (T3SS, a pathogenicity determinant of R. solanacearum) both in vitro and in planta, but it is T4P independent. We further revealed that TapV affected the T3SS expression via the PhcA-TapV-PrhG-HrpB pathway, consistent with previous reports that PhcA positively regulates expression of pilA and prhG. Moreover, deletion of tapV, but not rsp0189, significantly impaired the ability to migrate into and colonize xylem vessels of host plants, but there was no alteration in intercellular proliferation of R. solanacearum in tobacco leaves, which is similar to the pilA mutant. The tapV mutant showed significantly impaired virulence in host plants. This is the first report on the impact of T4P components on the T3SS, providing novel insights into our understanding of various biological functions of T4P and the complex regulatory pathway of T3SS in R. solanacearum.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/mpp.12930DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214476PMC
June 2020

Carbon substrate re-orders relative growth of a bacterium using Mo-, V-, or Fe-nitrogenase for nitrogen fixation.

Environ Microbiol 2020 04 29;22(4):1397-1408. Epub 2020 Feb 29.

Department of Geosciences, Princeton University, Princeton, NJ, 08544, USA.

Biological nitrogen fixation is catalyzed by the molybdenum (Mo), vanadium (V) and iron (Fe)-only nitrogenase metalloenzymes. Studies with purified enzymes have found that the 'alternative' V- and Fe-nitrogenases generally reduce N more slowly and produce more byproduct H than the Mo-nitrogenase, leading to an assumption that their usage results in slower growth. Here we show that, in the metabolically versatile photoheterotroph Rhodopseudomonas palustris, the type of carbon substrate influences the relative rates of diazotrophic growth based on different nitrogenase isoforms. The V-nitrogenase supports growth as fast as the Mo-nitrogenase on acetate but not on the more oxidized substrate succinate. Our data suggest that this is due to insufficient electron flux to the V-nitrogenase isoform on succinate compared with acetate. Despite slightly faster growth based on the V-nitrogenase on acetate, the wild-type strain uses exclusively the Mo-nitrogenase on both carbon substrates. Notably, the differences in H :N stoichiometry by alternative nitrogenases (~1.5 for V-nitrogenase, ~4-7 for Fe-nitrogenase) and Mo-nitrogenase (~1) measured here are lower than prior in vitro estimates. These results indicate that the metabolic costs of V-based nitrogen fixation could be less significant for growth than previously assumed, helping explain why alternative nitrogenase genes persist in diverse diazotroph lineages and are broadly distributed in the environment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1462-2920.14955DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187303PMC
April 2020

One-Pot Aqueous Synthesis of Icosahedral Au as Bifunctional Candidates for Enhanced Glucose Electrooxidation and Surface-Enhanced Raman Scattering.

ACS Appl Mater Interfaces 2020 Mar 25;12(10):12186-12194. Epub 2020 Feb 25.

School of Physics and Optoelectronic Engineering, Ludong University, Yantai 264025, China.

Bifunctional candidates, which could provide catalytic and plasmonic properties simultaneously, could activate a promising development for biomedicine. Here, we kinetically controlled and synthesized a penta-twinned icosahedral Au (Ih Au) by a facile wet-chemical protocol without assistance of stabilizers. Benefiting from icosahedral morphology and kinetic synthesis process, the Ih Au nanoparticles (NPs) incorporate three key advantages: (i) ample active sites/"hot spots" and surface strain, (ii) good stability/chemical inertness and easy functionalization, and (iii) biological compatibility and a clean surface, which could promote their electrocatalysis and photonic capacity. Ih Au NPs, as bifunctional nanomaterials, exert excellent electrocatalytic and surface-enhanced Raman scattering (SERS) performances. Ih Au delivers the highest glucose electrooxidation (GEO) peak current density with 6.87 mA cm, which is 14 times larger than that of Turkevich Au (0.49 mA cm) under the same condition. Moreover, the SERS signals of rhodamine 6G (R6G) on Ih Au are much stronger than that on the other corresponding Au counterparts. Particularly, the SERS intensity of R6G on Ih Au increases by about four times compared to that on Au NPs. This study motivates the great prospect for combining Ih Au's bifunctionalities and indicates the potential of bifunctional nanomaterials in biologically implanted devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.9b15715DOI Listing
March 2020

The structures and luminescence properties of SrGdNa(PO)F:Ce,Tb green phosphors with zero-thermal quenching of Tb for WLEDs.

Dalton Trans 2020 Jan;49(3):667-674

School of Physics and Optoelectronic Engineering, Ludong University, Yantai 264025, China.

In this paper, novel green-emitting Sr4Gd3Na3(PO4)6F2:Ce3+,Tb3+ phosphors with zero-thermal quenching of Tb3+ have been prepared and investigated. X-ray diffraction together with Rietveld structure refinement was employed to give an insight into the structures of the samples. The luminescence spectra at 300 K and 80 K confirmed that the Ce3+ ions in Sr4Gd3Na3(PO4)6F2:Ce3+ were distributed randomly on the cation sites and gave intense emission. Utilizing the Ce3+ → Tb3+ energy transfer, intense green emitting Sr4Gd3Na3(PO4)6F2:Ce3+,Tb3+ had been obtained. The energy migration mechanism of Ce3+ in Sr4Gd3Na3(PO4)6F2:Ce3+ and the energy transfer mechanism of Ce3+ → Tb3+ in Sr4Gd3Na3(PO4)6F2:Ce3+,Tb3+ were determined by the analysis of photoluminescence spectra and decay curves. Sr4Gd3Na3(PO4)6F2:Ce3+,Tb3+ exhibited a thermal-induced enhancement of Tb3+ emission at 25-300 °C, indicating an excellent thermal stability. Moreover, utilizing our prepared Sr4Gd3Na3(PO4)6F2:0.06Ce3+,0.15Tb3+ phosphor, green and white emitting LED devices have been fabricated. Our investigation indicated the potential application of prepared green phosphors in UV WLEDs and a feasible method to explore highly thermally stable phosphors utilizing the high thermal stability of donors together with energy transfer from donors to acceptors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9dt04320aDOI Listing
January 2020

Raspberry-Like Mesoporous ZnGaSiO:Cr Nanocarriers for Enhanced Near-Infrared Afterglow Imaging and Combined Cancer Chemotherapy.

ACS Appl Mater Interfaces 2019 Dec 21;11(48):44978-44988. Epub 2019 Nov 21.

Analytical & Testing Center , Sichuan University , Chengdu , Sichuan 610064 , China.

Near-infrared (NIR) persistent luminescence (PersL) nanoparticles based on trivalent chromium-doped gallates (ZGO) as nanocarriers show great potential for theranostics, owing to their autofluorescence-free background and deep tissue penetration. However, high drug loading capacity of ZGO nanocarriers remains a big challenge. Herein, raspberry-like mesoporous ZnGaSiO:Cr (designated as Si-ZGO) is first developed via a unique silica-assisted targeted etching strategy. The composition, morphology, NIR PersL capacities, and drug loading/releasing abilities of Si-ZGO have been explored. These results exhibit that Si-ZGO possess multiple inspiring characteristics including (i) spherical raspberry-like mesoporous morphology with a large cavity (total pore size ∼5.0 nm) and high specific surface area (∼80.653 m·g), promising excellent drug loading capacity (∼62 wt %); (ii) tunable sizes from 80 to 180 nm and improved aqueous-dispersibility, facilitating cellular uptake and permeation and retention (EPR) effect; (iii) new deep traps related to oxygen vacancies, achieving the brighter NIR PersL. These outstanding merits enable the further nanosystem ([email protected]) for proof-of-concept theranostics excellent chemotherapy effect, tumor-specific trackable ability, and pronounced NIR afterglow imaging in vivo. This work demonstrates the great potentials of Si-ZGO nanorasperries as a multifunctional theranostics platform, even more it hopefully could inspire other constructions of advanced functional materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.9b18124DOI Listing
December 2019

Temporal Coding of Single Auditory Nerve Fibers Is Not Degraded in Aging Gerbils.

J Neurosci 2020 01 12;40(2):343-354. Epub 2019 Nov 12.

Cluster of Excellence "Hearing4all" and Research Centre Neurosensory Science, Department of Neuroscience, School of Medicine and Health Science, Carl von Ossietzky University Oldenburg, 26129 Oldenburg, Germany

People suffering from age-related hearing loss typically present with deficits in temporal processing tasks. Temporal processing deficits have also been shown in single-unit studies at the level of the auditory brainstem, midbrain, and cortex of aged animals. In this study, we explored whether temporal coding is already affected at the level of the input to the central auditory system. Single-unit auditory nerve fiber recordings were obtained from 41 Mongolian gerbils of either sex, divided between young, middle-aged, and old gerbils. Temporal coding quality was evaluated as vector strength in response to tones at best frequency, and by constructing shuffled and cross-stimulus autocorrelograms, and reverse correlations, from responses to 1 s noise bursts at 10-30 dB sensation level (dB above threshold). At comparable sensation levels, all measures showed that temporal coding was not altered in auditory nerve fibers of aging gerbils. Furthermore, both temporal fine structure and envelope coding remained unaffected. However, spontaneous rates were decreased in aging gerbils. Importantly, despite elevated pure tone thresholds, the frequency tuning of auditory nerve fibers was not affected. These results suggest that age-related temporal coding deficits arise more centrally, possibly due to a loss of auditory nerve fibers (or their peripheral synapses) but not due to qualitative changes in the responses of remaining auditory nerve fibers. The reduced spontaneous rate and elevated thresholds, but normal frequency tuning, of aged auditory nerve fibers can be explained by the well known reduction of endocochlear potential due to strial dysfunction in aged gerbils. As our society ages, age-related hearing deficits become ever more prevalent. Apart from decreased hearing sensitivity, elderly people often suffer from a reduced ability to communicate in daily settings, which is thought to be caused by known age-related deficits in auditory temporal processing. The current study demonstrated, using several different stimuli and analysis techniques, that these putative temporal processing deficits are not apparent in responses of single-unit auditory nerve fibers of quiet-aged gerbils. This suggests that age-related temporal processing deficits may develop more central to the auditory nerve, possibly due to a reduced population of active auditory nerve fibers, which will be of importance for the development of treatments for age-related hearing disorders.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1523/JNEUROSCI.2784-18.2019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6948943PMC
January 2020

Modulating near-infrared persistent luminescence of core-shell nanoplatform for imaging of glutathione in tumor mouse model.

Biosens Bioelectron 2019 Nov 3;144:111671. Epub 2019 Sep 3.

Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan, 610064, China. Electronic address:

Developing near-infrared (NIR) persistent luminescence (PersL) bioprobes has attracted increasing attention due to their ultra-low autofluorescence and deep tissue penetration. Accurate imaging of glutathione (GSH) in vivo is of great significance to provide a real-time visualization of pathologies. Herein, manipulating metal ions as the switch of NIR afterglow was demonstrated to construct NIR PersL nanoprobes for the first time, which was highly efficient and much simpler than the luminescence resonance energy transfer (LRET) principle. As a proof-of-concept application, an activatable NIR PersL nanoprobe (Probe-1) built on core-shell structure was rationally fabricated for detection of GSH via employing Cu as the quencher. In light of unique NIR PersL feature and flexible functionality, Probe-1 exhibited greatly enhanced analytical performances for GSH detection, and particularly imaging of tumor in living mouse. This approach without limits of spectral overlap from the LRET principle provided a new way to develop NIR PersL bioprobes for autofluorescence-free detection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2019.111671DOI Listing
November 2019

Effect of Expression Levels on Preadipocyte Differentiation in Chinese Red Steppe Cattle.

DNA Cell Biol 2019 Sep 29;38(9):945-954. Epub 2019 Jul 29.

Branch of Animal Husbandry, Jilin Academy of Agricultural Science, Gongzhuling, China.

Domestic cattle are an important type of livestock, with beef production playing a major role in the agricultural economy. Adipocyte levels and fat content are interrelated, with meat quality being highly dependent on its fat content and distribution. Acyl-CoA synthetases of long-chain (ACSL) fatty acids (FAs) play an integral role in virtually every metabolic pathway in mammalian biochemistry, including complex lipid biosynthesis, protein modification, and β-oxidation processes. activity is also known to be associated with adipocyte differentiation; however, its biological mechanism of action is currently unclear. Gene expression in subcutaneous preadipocytes isolated from subcutaneous deposits of Chinese Red Steppe cattle has been studied using cell transfection, real-time polymerase chain reaction and western blot analysis. The lipid and triglyceride contents of lipid droplets have also been measured to verify the levels of gene expression. These combined studies show that is induced during adipocyte differentiation, with its overexpression promoting an increase in the triglyceride content of lipid droplets. Furthermore, mRNA and protein expression levels for adipocyte differentiation marker genes, such as peroxisome proliferator-activated receptor gamma () and CCAAT/enhancer-binding protein alpha (), were markedly increased during mature adipocyte cell differentiation. Knockdown of expression using ACSL3 small interfering RNAs (siRNAs) resulted in a decrease in lipid content of cattle adipocytes, providing further evidence that plays a key role in the differentiation process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/dna.2018.4443DOI Listing
September 2019

The protective effects of bone mesenchymal stem cells on paraquat-induced acute lung injury via the muc5b and ERK/MAPK signaling pathways.

Am J Transl Res 2019 15;11(6):3707-3721. Epub 2019 Jun 15.

Department of Emergency, Shengjing Hospital Affiliated to China Medical University No. 36 Sanhao Street, Heping District, Shenyang 110004, Liaoning Province, China.

Objective: To evaluate the protective effect of bone mesenchymal stem cells (BMSCs) on paraquat (PQ)-induced acute lung injury (ALI) and investigate the possible underlying mechanisms.

Methods: Male Sprague Dawley rats were treated with BMSCs (3 × 10) 1 h after intraperitoneal injection of PQ. The cell apoptosis rate and mitochondrial membrane potential in rat pulmonary alveolar type II epithelial (ATII) cells were quantitated by flow cytometry. IL-17, IL-6, and MUC5B levels in bronchoalveolar lavage fluid (BALF) and ATII culture medium were measured. Lung tissues were collected to determine the wet-to-dry (W/D) ratios and lung injury scores, in addition to the protein and mRNA expression levels of ERK1/2, Bcl-2, Bax, and muc5b.

Results: BMSCs had decreased mRNA expression of Muc5b in lung tissue of rats with PQ-induced ALI as shown by RNA-seq. Treatment with BMSCs also alleviated the PQ-induced increases in protein expression in the BALF and reduced the concentration of IL-17, IL-6, and Muc5b in both the BALF and ATII culture medium. In addition, the ATII cell apoptosis rate and mitochondrial membrane potential, as well as the W/D ratios, were decreased by BMSC treatment. Moreover, BMSCs ameliorated the expression levels of mRNA and active caspase-3 proteins and increased mRNA expression. Furthermore, BMSCs attenuated ERK1/2 activation upon PQ-induced ALI in lung tissue.

Conclusion: BMSC therapy can protect against PQ-induced ALI in rats. A possible mechanism is the suppression of the muc5b and ERK/MAPK signaling pathways, resulting in an improvement in the endothelial permeability and a decrease in inflammation and cell apoptosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6614636PMC
June 2019

Nitrogen sourcing during viral infection of marine cyanobacteria.

Proc Natl Acad Sci U S A 2019 07 15;116(31):15590-15595. Epub 2019 Jul 15.

Department of the Geophysical Sciences, University of Chicago, Chicago, IL 60637.

The building blocks of a virus derived from de novo biosynthesis during infection and/or catabolism of preexisting host cell biomass, and the relative contribution of these 2 sources has important consequences for understanding viral biogeochemistry. We determined the uptake of extracellular nitrogen (N) and its biosynthetic incorporation into both virus and host proteins using an isotope-labeling proteomics approach in a model marine cyanobacterium WH8102 infected by a lytic cyanophage S-SM1. By supplying dissolved N as N postinfection, we found that proteins in progeny phage particles were composed of up to 41% extracellularly derived N, while proteins of the infected host cell showed almost no isotope incorporation, demonstrating that de novo amino acid synthesis continues during infection and contributes specifically and substantially to phage replication. The source of N for phage protein synthesis shifted over the course of infection from mostly host derived in the early stages to more medium derived later on. We show that the photosystem II reaction center proteins D1 and D2, which are auxiliary metabolic genes (AMGs) in the S-SM1 genome, are made de novo during infection in an apparently light-dependent manner. We also identified a small set of host proteins that continue to be produced during infection; the majority are homologs of AMGs in S-SM1 or other viruses, suggesting selective continuation of host protein production during infection. The continued acquisition of nutrients by the infected cell and their utilization for phage replication are significant for both evolution and biogeochemical impact of viruses.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1073/pnas.1901856116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6681717PMC
July 2019

Identification of microRNA-21 target genes associated with hair follicle development in sheep.

PeerJ 2019 27;7:e7167. Epub 2019 Jun 27.

Jilin Academy of Agricultural Science, Branch of Animal Husbandry, Gongzhuling, China.

Aim: The target molecule regulatory function of microRNA-21 (miR-21) in multiple signalling pathways has become a main focus of genetic and pharmacological regulatory studies of various diseases. The identification of target genes for miRNA-21 in the development of hair follicles can provide new research pathways for the regulation of cell development.

Methods: In the present study, eight six-month-old ewes from Super Merino (SM) and Small Tailed Han (STH) sheep breeds were selected. Target prediction and dual-luciferase wild-type and mutant vectors were used to identify the target genes of miR-21. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) and bioinformatics analysis were conducted to analyze the effects of miR-21.

Results: The results show that the expressions of , and were downregulated by miRNA-21 at rates of 36%, 26% and 48%, respectively. Moreover, there was a significant negative correlation between the expression of miR-21 and the three target genes in sheep with two extreme phenotypes. The expression of microRNA-21in October was significantly lower than that in January and February; while the expression of , and in October was higher than that in January and February. Conclusions: These results suggest that , and are three newly discovered target genes of miR-21 and might be involved in the effects of miR-21 on hair follicle development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.7167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6599667PMC
June 2019

Organosiloxane and Polyhedral Oligomeric Silsesquioxanes Compounds as Chemiluminescent Molecular Probes for Direct Monitoring Hydroxyl Radicals.

Anal Chem 2019 07 28;91(14):8926-8932. Epub 2019 Jun 28.

Despite the tremendous progress in the research of luminescent probes for reactive oxygen species (ROS), designing luminescent ROS probes with high sensitivity for the individual ROS is still retarded because of their high reactivity and the rapid and complex interconversion reactions among them. Herein, organosiloxane and polyhedral oligomeric silsesquioxanes (POSS) compounds are designed as a novel class of luminescent molecular probes to produce extraordinary chemiluminescence (CL) based on the specific electrophilic attack of OH. No CL signal can be obtained by the other ROS and strong oxidants. AEAP-POSS formed by hydrolytical condensation of 3-(2-aminoethylamino)propyl]trimethoxysilane (AEAPTMS) is constructed to covalently link a dye molecular, perylene diimide derivative (PDI), and an intramolecular chemiluminescence resonance energy transfer (CRET) system is obtained to realize the red shift of CL wavelength and enhanced CL intensity. This probe based on CRET is applied to monitor inherent OH in ambient particular matter (PM and PM). Density functional theory (DFT), ion chromatograph, X-ray photoelectron spectroscopy (XPS), particle size analysis, and fluorescence spectrum (FL) are applied to study the CL mechanism. These studies discover that electronically carbonyl CHCO is the CL emitter, and the silicon-oxygen skeleton in the organosiloxane and POSS compounds plays the key role in undergoing chemiluminescence (CL) reaction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.analchem.9b00637DOI Listing
July 2019

Fluorescence nano metal organic frameworks modulated by encapsulation for construction of versatile biosensor.

Talanta 2019 Aug 2;201:96-103. Epub 2019 Apr 2.

Analytical & Testing Center, Sichuan University, Chengdu, Sichuan, 610064, China; Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan, 610064, China. Electronic address:

The versatile fluorescence biosensors were implemented based on multipurpose nano metal organic frameworks (nMOFs) nanocomposites, which were obtained as fluorescence signal probes through encapsulation nMOFs. In this modulation process, the encapsulant bind with nMOFs through electrostatic attraction and hydrogen bond. Meanwhile, fluorescence enhancement of nMOFs were observed, accompanied by an increased in water solubility. The specific combination of target analyte triggers the release of the encapsulant and allows the target analyte to bind encapsulant to form a stable analyte-encapsulant complex. The complex binds tightly and promotes the photoinduced electron transfer from MOFs nanocomposites to the complex, thus resulting in reduce the fluorescence intensity of the MOFs nanocomposites. The results indicated that this novel system enables the specific and versatile detection of target biomolecules such as hemin and folic acid with high sensitivity and selectivity based on the choices of different encapsulant. Under optimized conditions, these biosensors show high sensitivity with a linear range from 0.16 to 12.5 μg/mL for hemin and 0.15-17.5 μmol/L for folic acid and detection limit of 47 ng/mL for hemin and 45 nmol/L folic acid. Moreover, it exhibits good performance of excellent selectivity, high stability, and acceptable fabrication reproducibility. This new multipurpose MOFs nanocomposites open avenues for combining together their properties and functionalities, and displaying novel important applications in fluorescence biosensors.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.talanta.2019.03.107DOI Listing
August 2019

Adipogenesis associated Mth938 domain containing (AAMDC) protein expression is regulated by alternative polyadenylation and microRNAs.

FEBS Lett 2019 07 4;593(14):1724-1734. Epub 2019 Jun 4.

College of Life Science, Liaocheng University, China.

The post-transcriptional events regulating expression of the adipogenesis associated Mth938 domain containing (AAMDC) protein are poorly understood. Here, we find that AAMDC expresses three isoforms due to alternative polyadenylation (APA) and alternative splicing (AS). Luciferase assay revealed that expression of the two isoforms with short and long 3'UTRs is differentially controlled. Further analyses showed that the short isoform displays higher translation efficiency and that miR-2428/664a reduces expression of the long isoform, indicating that APA-mediated shortening of the AAMDC 3'UTR renders the short isoform insusceptible to miRNA-mediated suppression due to loss of the binding sites for miR-2428/664a. Finally, we demonstrate that the short AAMDC isoform promotes bovine preadipocyte differentiation. Collectively, our findings indicate that AAMDC is post-transcriptionally regulated through APA and microRNAs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/1873-3468.13449DOI Listing
July 2019

[Sepsis after elective septoplasty and concha nasales inferior reduction].

Laryngorhinootologie 2019 Aug 26;98(8):571-574. Epub 2019 Apr 26.

CharitéCentrum 13 für Innere Medizin mit Gastroenterologie und Nephrologie Charité.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1055/a-0889-8532DOI Listing
August 2019

Quantum dots-based chemiluminescence probes: an overview.

Luminescence 2019 Sep 25;34(6):530-543. Epub 2019 Apr 25.

College of Chemistry, Sichuan University, Chengdu, Sichuan, China.

This mini-review describes the recent developments in quantum dots-based nanoprobes in liquid-phase chemiluminescence (CL) analysis. In the referenced reports, multiple quantum dots (QDs) were adopted as final emission species either after direct oxidation reactions (direct CL) or after chemiluminescence resonance energy transfer (indirect CL). This review does not include papers in which QDs have been used as enhancers, catalysts, carriers or quenchers in chemiluminescence systems. A brief overview on the CL mechanisms of various QDs-based nanoprobes and their analytical applications over the last decade is given, followed by comments on the future challenges and prospects in this field.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/bio.3633DOI Listing
September 2019

Growth and optoelectronic application of CsPbBr thin films deposited by pulsed-laser deposition.

Opt Lett 2019 Apr;44(8):1908-1911

All-inorganic perovskite CsPbBr thin films have been prepared on Si (100) substrate by a pulsed-laser deposition (PLD) technique, and the morphology, structure, absorbance, and photoluminescence properties of CsPbBr thin films are investigated. A photodetector based on CsPbBr/n-Si heterojunction has been fabricated, and the performances of the device are characterized. The heterojunction photodetector exhibits diode-like rectifying behavior, and the photocurrent-to-dark-current ratio and peak responsivity of the heterojunction are approximately 168.5 and 0.6 A/W (-5  V, 520 nm), respectively. Furthermore, the CsPbBr/n-Si heterojunction photodetector exhibits fast response and recovery times. With good optoelectronic properties, CsPbBr thin films prepared by PLD should be widely applicable to high-performance photodetectors and other optoelectronic devices.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OL.44.001908DOI Listing
April 2019

Mesenchymal Stem Cells Alleviate Acute Lung Injury and Inflammatory Responses Induced by Paraquat Poisoning.

Med Sci Monit 2019 Apr 10;25:2623-2632. Epub 2019 Apr 10.

Emergency Department, Shengjing Hospital Affiliated to China Medical University, Shenyang, Liaoning, China (mainland).

BACKGROUND Mesenchymal stem cells (MSCs) show anti-oxidative and anti-inflammatory effects that have prompted further research into their potential applications in treating paraquat (PQ) poisoning cases in emergency rooms. We assessed the protective effects, underlying mechanisms, and secondary inflammatory responses of MSCs on PQ-induced acute lung injury. MATERIAL AND METHODS Sprague-Dawley rats were injected intraperitoneally with PQ (20 µg per gram of body weight). MSCs were injected through the caudal vein 1 h after PQ treatment. The severity of lung injury and oxidative stress and levels of inflammatory mediators were examined with and without MSC grafting. Expression levels of TLR4, NF-kappaB, p65, Nrf2, HO-1, and activated caspase-3 protein were determined by Western blotting. RESULTS Administration of MSCs significantly decreased the levels of TNF-alpha, IL-1ß, and IL-6 and polymorphonuclear neutrophil (PMN) count in the bronchoalveolar lavage fluid (BALF) of rats with PQ-induced ALI. In addition, MSC also effectively reduced the wet-to-dry lung weight ratio, lung injury score, and the levels of MDA and 8-OHdG. Conversely, MSC increased SOD and GSH-PX activity in the lung tissue. Moreover, MSC significantly upregulated HO-1, Nrf-2 protein expression in the lung tissue. In contrast, the levels of TLR4, NF-kappaB p65 and activated caspase-3 protein were decreased in MSC-treated rats (P<0.05). CONCLUSIONS Treatment with MSCs overexpressed Nrf2 gene and activated downstream antioxidant HO-1, leading to inhibit oxidative stress, cell apoptosis and inflammatory response in lung tissue, thereby significantly improving PQ-induced acute lung injury in rats.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12659/MSM.915804DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474293PMC
April 2019