Publications by authors named "Lichun Wang"

160 Publications

Serum levels of irisin in postmenopausal women with osteoporotic hip fractures.

Cytokine 2021 Dec 21;148:155708. Epub 2021 Sep 21.

Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. Electronic address:

Objective: The purpose of this study was to evaluate the role of circulating serum levels of irisin in predicting hip fracture occurrence in a cohort of Chinese postmenopausal women.

Methods: This was a cross-section and case-control study. Four hundred and thirty postmenopausal women aged 50-90 years were included (215 with hip fractures and 215 age-matched cases without fracture). Clinical features, bone mineral density (BMD) and serum biomarkers levels including irisin were measured at baseline. Cox proportional hazards regression analysis was used to assess the correlation between irisin and fracture risk.

Results: The mean age of those participants was 68.7 (S.D. 11.7) and 53.0% were order than 65. The irisin serum levels were positively related to total body BMD and total hip BMD. Women with hip fractures showed lower mean serum levels of irisin compared normal control women (457.6 ± 172.6 ng/ml vs. 602.2 ng/ml; P < 0.001). The irisin levels in third and fourth quartiles were associated with the risk of hip fracture (the lowest quartile of irisin levels as the reference), and risk of fracture reduced by 67% (hazard ratio [HR] = 0.33; 95 %CI: 0.18-0.54; P < 0.001) and 84% (HR = 0.16; 95 %CI: 0.09-0.29; P < 0.001). The irisin levels in third and fourth quartiles were also associated with the risk of osteoporosis, and risk of fracture reduced by 55% (HR = 0.45; 95 %CI: 0.21-0.63; P = 0.003) and 73% (HR = 0.27; 95 %CI: 0.15-0.47; P < 0.001).

Conclusion: Decreased serum levels of circulating irisin are associated with high risk of osteoporosis-related hip fractures and osteoporosis.
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http://dx.doi.org/10.1016/j.cyto.2021.155708DOI Listing
December 2021

Immunological evaluation of an inactivated SARS-CoV-2 vaccine in rhesus macaques.

Mol Ther Methods Clin Dev 2021 Dec 26;23:108-118. Epub 2021 Aug 26.

Institute of Medical Biology, Chinese Academy of Medicine Sciences & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Kunming 650118, China.

Because of the relatively limited understanding of coronavirus disease 2019 (COVID-19) pathogenesis, immunological analysis for vaccine development is needed. Mice and macaques were immunized with an inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine prepared by two inactivators. Various immunological indexes were tested, and viral challenges were performed on day 7 or 150 after booster immunization in monkeys. This inactivated SARS-CoV-2 vaccine was produced by sequential inactivation with formaldehyde followed by propiolactone. The various antibody responses and specific T cell responses to different viral antigens elicited in immunized animals were maintained for longer than 150 days. This comprehensive immune response could effectively protect vaccinated macaques by inhibiting viral replication in macaques and substantially alleviating immunopathological damage, and no clinical manifestation of immunopathogenicity was observed in immunized individuals during viral challenge. This candidate inactivated vaccine was identified as being effective against SARS-CoV-2 challenge in rhesus macaques.
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http://dx.doi.org/10.1016/j.omtm.2021.08.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387130PMC
December 2021

Real-Time Human Action Recognition Using Locally Aggregated Kinematic-Guided Skeletonlet and Supervised Hashing-by-Analysis Model.

IEEE Trans Cybern 2021 Aug 26;PP. Epub 2021 Aug 26.

3-D action recognition is referred to as the classification of action sequences which consist of 3-D skeleton joints. While many research works are devoted to 3-D action recognition, it mainly suffers from three problems: 1) highly complicated articulation; 2) a great amount of noise; and 3) low implementation efficiency. To tackle all these problems, we propose a real-time 3-D action-recognition framework by integrating the locally aggregated kinematic-guided skeletonlet (LAKS) with a supervised hashing-by-analysis (SHA) model. We first define the skeletonlet as a few combinations of joint offsets grouped in terms of the kinematic principle and then represent an action sequence using LAKS, which consists of a denoising phase and a locally aggregating phase. The denoising phase detects the noisy action data and adjusts it by replacing all the features within it with the features of the corresponding previous frame, while the locally aggregating phase sums the difference between an offset feature of the skeletonlet and its cluster center together over all the offset features of the sequence. Finally, the SHA model combines sparse representation with a hashing model, aiming at promoting the recognition accuracy while maintaining high efficiency. Experimental results on MSRAction3D, UTKinectAction3D, and Florence3DAction datasets demonstrate that the proposed method outperforms state-of-the-art methods in both recognition accuracy and implementation efficiency.
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http://dx.doi.org/10.1109/TCYB.2021.3100507DOI Listing
August 2021

Bio-inspired dual-functional phospholipid-poly(acrylic acid) brushes grafted porous poly(vinyl alcohol) beads for selective adsorption of low-density lipoprotein.

J Mater Chem B 2021 Aug 23;9(32):6364-6376. Epub 2021 Jul 23.

Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China.

Elevated levels of low-density lipoproteins (LDL) are recognized as a crucial indicator of hyperlipidemia (HLP) and lowering of LDL levels represents an effective clinical treatment strategy. Inspired by the conjugation of phospholipid monolayers and the lipid content of the LDL particle, the current study describes the preparation of an innovative hemoperfusion adsorbent. The adsorbent was prepared by attachment of phosphatidyl ethanolamine to poly(acrylic acid) modified poly(vinyl alcohol-co-triallyl isocyanurate) beads ([email protected]). The interaction between LDL and adsorbent mimics the lipoprotein microemulsion present in the blood and thus promotes efficient binding with high affinity. In vitro adsorption using serum from patients with HLP revealed that the LDL adsorption of [email protected] was 4.44 times higher than that of controls and the removal rate of LDL using [email protected] was about twice as high as that of the anti-atherogenic high-density lipoprotein (HDL). In vivo whole blood perfusion demonstrated the superior affinity of [email protected] for LDL since LDL concentration was significantly reduced from 10.71 ± 2.36 mmol L to 6.21 ± 1.45 mmol L, while the HDL level was not severely reduced (from 0.98 ± 0.12 mmol L to 0.56 ± 0.15 mmol L). Additionally, [email protected] exhibited excellent hemocompatibility and low cytotoxicity. Therefore, [email protected] is a potential adsorbent for whole blood perfusion to treat hyperlipidemia.
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http://dx.doi.org/10.1039/d1tb01220gDOI Listing
August 2021

Long-Term Visit-to-Visit Mean Arterial Pressure Variability and the Risk of Heart Failure and All-Cause Mortality.

Front Cardiovasc Med 2021 4;8:665117. Epub 2021 Jun 4.

Department of Cardiology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.

Systolic or diastolic blood pressure (BP) variability is associated with an increased risk of cardiovascular events. We assessed whether BP variability measured by mean arterial pressure (MAP) was associated with increased risk of heart failure (HF) and death in individuals with or without hypertension. We evaluated 9,305 Atherosclerosis Risk in Communities (ARIC) study participants with or without hypertension and calculated BP variability based on MAP values from visit 1 to 4 [expressed as standard deviation (SD), average real variability (ARV), coefficient of variation (CV), and variability independent of the mean (VIM)]. Multivariate-adjusted Cox regression model and restricted cubic spline curve were used to evaluate the associations of MAP variability with all-cause mortality and HF. During a median follow-up of 16.8 years, 1,511 had an HF event and 2,903 died. Individuals in the highest quartile of VIM were both associated with a 21% higher risk of all-cause mortality [hazard ratio (HR), 1.21; 95% CI, 1.09-1.35] and HF (HR, 1.21; 95% CI, 1.04-1.39) compared with the lowest quartile of VIM. Cubic spline curves reveal that the risk of deaths and HF increased with MAP variability when it reached a higher level. Results were similar in individuals with normotension (all-cause mortality: HR, 1.30; 95% CI, 1.09-1.55; HF, HR, 1.49; 95% CI, 1.12-1.98). In individuals with or without hypertension, greater visit-to-visit MAP variability was associated with a higher risk of all-cause mortality and HF, indicating that the BP variability assessed by MAP might be a potential risk factor for HF and death.
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http://dx.doi.org/10.3389/fcvm.2021.665117DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211989PMC
June 2021

Immunological Identification and Characterization of the Capsid Scaffold Protein Encoded by UL26.5 of Herpes Simplex Virus Type 2.

Front Cell Infect Microbiol 2021 26;11:649722. Epub 2021 May 26.

Institute of Medical Biology, Chinese Academy of Medicine Sciences & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development for Severe Infectious Diseases, Kunming, China.

Herpes simplex virus type 2 (HSV2), a pathogen that causes genital herpes lesions, interferes with the host immune system various known and unknown mechanisms. This virus has been used to study viral antigenic composition. Convalescent serum from HSV2-infected patients was used to identify viral antigens 2-D protein electrophoresis and immunoblotting. The serum predominantly recognized several capsid scaffold proteins encoded by gene UL26.5, mainly ICP35. This protein has been primarily reported to function temporarily in viral assembly but is not expressed in mature virus particles. Further immunological studies suggested that this protein elicits specific antibody and cytotoxic T lymphocyte (CTL) responses in mice, but these responses do not result in a clinical protective effect in response to HSV2 challenge. The data suggested that immunodominance of ICP35 might be used to design an integrated antigen with other viral glycoproteins.
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http://dx.doi.org/10.3389/fcimb.2021.649722DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187855PMC
July 2021

An electrographic AV optimization for the maximum integrative atrioventricular and ventricular resynchronization in CRT.

BMC Cardiovasc Disord 2021 06 10;21(1):288. Epub 2021 Jun 10.

Department of Cardiology, The First Affiliated Hospital of Sun Yat-Sen University, No. 58, Zhongshan 2nd Rd, Guangzhou, 510080, Guangdong, People's Republic of China.

Background: Atrioventricular (AV) delay could affect AV and ventricular synchrony in cardiac resynchronization therapy (CRT). Strategies to optimize AV delay according to optimal AV synchrony (AV) or ventricular synchrony (AV) would potentially be discordant. This study aimed to explore a new AV delay optimization algorithm guided by electrograms to obtain the maximum integrative effects of AV and ventricular resynchronization (opt-AV).

Methods: Forty-nine patients with CRT were enrolled. AV was measured through the Ritter method. AV was obtained by yielding the narrowest QRS. The opt-AV was considered to be AV or AV when their difference was < 20 ms, and to be the AV delay with the maximal aortic velocity-time integral between AV and AV when their difference was > 20 ms.

Results: The results showed that sensing/pacing AV (SAV/PAV) were correlated with atrial activation time (P/P) (P < 0.05). Sensing/pacing AV (SAV/PAV) was correlated with the intrinsic AV conduction time (As-Vs/Ap-Vs) (P < 0.01). The percentages of patients with more than 20 ms differences between SAV/PAV and SAV/PAV were 62.9% and 57.1%, respectively. Among them, opt-AV was linearly correlated with SAV/PAV and SAV/PAV The sensing opt-AV (opt-SAV) = 0.1 × SAV + 0.4 × SAV + 70 ms (R = 0.665, P < 0.01) and the pacing opt-AV (opt-PAV) = 0.25 × PAV + 0.5 × PAV + 30 ms (R = 0.560, P < 0.01).

Conclusion: The SAV/PAV and SAV/PAV were correlated with the atrial activation time and the intrinsic AV conduction interval respectively. Almost half of the patients had a > 20 ms difference between SAV/PAV and SAV/PAV. The opt-AV could be estimated based on electrogram parameters.
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http://dx.doi.org/10.1186/s12872-021-02096-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193898PMC
June 2021

Hydroxyapatite reinforced inorganic-organic hybrid nanocomposite as high-performance adsorbents for bilirubin removal and in pig models.

Bioact Mater 2021 Dec 24;6(12):4772-4785. Epub 2021 May 24.

Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China.

Highly efficient removal of bilirubin from whole blood directly by hemoperfusion for liver failure therapy remains a challenge in the clinical field due to the low adsorption capacity, poor mechanical strength and low biocompatibility of adsorbents. In this work, a new class of nanocomposite adsorbents was constructed through an inorganic-organic co-crosslinked nanocomposite network between vinyltriethoxysilane (VTES)-functionalized hydroxyapatite nanoparticles (V-Hap) and non-ionic styrene-divinylbenzene (PS-DVB) resins (PS-DVB/V-Hap) using suspension polymerization. Notably, our adsorbent demonstrated substantially improved mechanical performance compared to the pure polymer, with the hardness and modulus increasing by nearly 3 and 2.5 times, respectively. Moreover, due to the development of a mesoporous structure, the prepared PS-DVB/V-Hap3 exhibited an ideal adsorption capacity of 40.27 mg g. More importantly, the obtained adsorbent beads showed outstanding blood compatibility and biocompatibility. Furthermore, extracorporeal hemoperfusion verified the efficacy and biosafety of the adsorbent for directly removing bilirubin from whole blood in pig models, and this material could potentially prevent liver damage and improve clinical outcomes. Taken together, the results suggest that PS-DVB/V-Hap3 beads can be used in commercial adsorption columns to threat hyperbilirubinemia patients through hemoperfusion, thus replacing the existing techniques where plasma separation is initially required.
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http://dx.doi.org/10.1016/j.bioactmat.2021.05.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144535PMC
December 2021

Soil bacterial community dynamics following bioaugmentation with Paenarthrobacter sp. W11 in atrazine-contaminated soil.

Chemosphere 2021 Nov 28;282:130976. Epub 2021 May 28.

Institute of Agricultural Resources and Environment, Jilin Academy of Agricultural Sciences, 1363 Shengtai Street, Changchun, 130033, China. Electronic address:

Atrazine is one of the most widely used herbicides, however it and its metabolites cause widespread contamination in soil and ground water. Bioaugmentation is an effective method for remediation of environmental organic pollutants. High-throughput sequencing provides an important tool for understanding the changes of microbial community and function in response to pollutants degradation based on bioaugmentation. In this study, the effect of biodegradation with Paenarthrobacter sp. W11 and the change of microbial community during atrazine degradation were investigated. The results showed that bioaugmentation significantly accelerated the degradation rate of atrazine in soil and reduced the toxic effect of atrazine residues on wheat growth. The extra available NH through atrazine mineralization could serve as a nitrogen source to increase microbial numbers. High-throughput sequencing further revealed that the microbial community restored a new balance. The function of microbial community predicted by PICRUSt2 suggested that the biodegradation process of atrazine affected not only the atrazine degradation pathway, but also the nitrogen metabolism pathway. Methylobacillus and Pseudomonas were considered as the most important indigenous atrazine-degrading microorganisms, because their relative abundances were positively correlated with the relative abundance of Paenarthrobacter and atrazine degradation pathway. This study provides insight into the cooperation between indigenous microorganisms and external inoculums on atrazine degradation process.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130976DOI Listing
November 2021

Intensified antibody response elicited by boost suggests immune memory in individuals administered two doses of SARS-CoV-2 inactivated vaccine.

Emerg Microbes Infect 2021 Dec;10(1):1112-1115

Institute of Medical Biology, Chinese Academy of Medicine Sciences and Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development for Severe Infectious Diseases, Kunming, People's Republic of China.

Neutralizing antibodies in the subjects of an inactivated SARS-CoV-2 vaccine clinical trial showed a decreasing trend over months. An investigation studying the third immunization suggested that the waning of neutralizing antibodies in individuals administered two doses of inactivated vaccine does not mean the disappearance of immunity.
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http://dx.doi.org/10.1080/22221751.2021.1937328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204957PMC
December 2021

Implantable platinum nanotree microelectrode with a battery-free electrochemical patch for peritoneal carcinomatosis monitoring.

Biosens Bioelectron 2021 Aug 22;185:113265. Epub 2021 Apr 22.

Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, PR China. Electronic address:

As a severe stage of cancers, peritoneal carcinomatosis should be frequently monitored by means of ascites analysis. Nevertheless, the analysis process is traumatic and time-consuming in clinical practice. In this study, an implantable platinum nanotree microelectrode with a wireless, battery-free and flexible electrochemical patch was developed for in vivo and real-time peritoneal glucose detection to monitor peritoneal carcinomatosis. As the core of implantable microelectrode, platinum trees were synthesized by one-step electrodeposition method and highly sensitive to glucose detection. The platinum nanotree microelectrode was implantable in peritoneal cavity in minimally invasive way. A flexible circuit patch could execute electrochemical test and realize wireless power harvesting and data interaction with a near field communication (NFC)-enabled smartphone. The whole system could detect glucose dynamics in vivo in rat peritoneal cavity. Furthermore, the accuracy of this system was validated in ascites of patients. In this way, the system could offer hassle-free, rapid and minimally invasive opportunities toward peritoneal carcinomatosis monitoring.
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http://dx.doi.org/10.1016/j.bios.2021.113265DOI Listing
August 2021

SARS-CoV-2 inactivated vaccine (Vero cells) shows good safety in repeated administration toxicity test of Sprague Dawley rats.

Food Chem Toxicol 2021 Jun 24;152:112239. Epub 2021 Apr 24.

Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, China; Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Diseases, Kunming, 650118, China. Electronic address:

The outbreak of COVID-19 has posed a serious threat to global public health. Vaccination may be the most effective way to prevent and control the spread of the virus. The safety of vaccines is the focus of preclinical research, and the repeated dose toxicity test is the key safety test to evaluate the vaccine before clinical trials. The purpose of this study was (i) to observe the toxicity and severity of an inactivated SARS-CoV-2 vaccine (Vero cells) in rodent Sprague Dawley rats after multiple intramuscular injections under the premise of Good Laboratory Practice principles and (ii) to provide a basis for the formulation of a clinical trial scheme. The results showed that all animals in the experimental group were in good condition, no regular changes related to the vaccine were found in the detection of various toxicological indexes, and no noticeable stimulating reaction related to the vaccine was found in the injected local tissues. The neutralizing antibodies in the low- and high-dose vaccine groups began to appear 14 days after the last administration. In the negative control group, no neutralizing antibodies were observed from the administration period to the recovery period. Therefore, the repeated administration toxicity test of the inactivated SARS-CoV-2 vaccine (Vero cells) in Sprague Dawley rats showed no obvious toxic reaction. It was preliminarily confirmed that the vaccine can stimulate production of neutralizing antibodies and is safe in Sprague Dawley rats.
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http://dx.doi.org/10.1016/j.fct.2021.112239DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064818PMC
June 2021

Appraisal of Guidelines for the Management of Blood Pressure in Patients with Diabetes Mellitus: The Consensuses, Controversies and Gaps.

Diabetes Metab J 2021 Sep 23;45(5):753-764. Epub 2021 Apr 23.

Department of Cardiology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.

Background: Currently available guidelines contain conflicting recommendations on the management of blood pressure (BP) in patients with diabetes mellitus (DM). Therefore, it is necessary to appraise the guidelines and summarize the agreements and differences among recommendations.

Methods: Four databases and the websites of guideline organizations were searched for guidelines regarding BP targets and thresholds for pharmacologic therapy in DM patients, and the included guidelines were appraised with the Appraisal of Guidelines for Research and Evaluation (AGREE) II instrument.

Results: In 6,498 records identified, 20 guidelines met our inclusion criteria with 64.0% AGREE II scores (interquartile range, 48.5% to 72.0%). The scores of the European and American guidelines were superior to those of the Asian guidelines (both adjusted P<0.001). Most of the guidelines advocated systolic BP targets <130 mm Hg (12 guidelines, 60%) and diastolic BP targets <80 mm Hg (14 guidelines, 70%) in DM patients. Approximately half of the guidelines supported systolic BP thresholds >140 mm Hg (10 guidelines, 50%) and diastolic BP thresholds >90 mm Hg (nine guidelines, 45%). The tiny minority of the guidelines provided the relevant recommendations regarding the lower limit of official BP targets and the ambulatory BP monitoring (ABPM)/home BP monitoring (HBPM) targets and thresholds in DM patients.

Conclusion: The lower official BP targets (<130/80 mm Hg) in patients with DM are advocated by most of the guidelines, but they contain conflicting recommendations on the official BP thresholds. Moreover, the gaps regarding the lower limit of official BP targets and the ABPM/HBPM targets and thresholds need to be considered by future study.
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http://dx.doi.org/10.4093/dmj.2020.0134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8497930PMC
September 2021

The safety and immunogenicity of an inactivated SARS-CoV-2 vaccine in Chinese adults aged 18-59 years: A phase I randomized, double-blinded, controlled trial.

Vaccine 2021 05 9;39(20):2746-2754. Epub 2021 Apr 9.

Institute of Medical Biology, Chinese Academy of Medicine Sciences & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development for Severe Infectious Diseases, Kunming 650118, China.

Background: This study examined the safety and immunogenicity of an inactivated SARS-CoV-2 vaccine.

Method: In a phase I randomized, double-blinded, placebo-controlled trial involving 192 healthy adults 18-59 years old, two injections of three doses (50 EU, 100 EU, 150 EU) of an inactivated SARS-CoV-2 vaccine or placebo were administered intramuscularly at a 2- or 4-week interval. The safety and immunogenicity of the vaccine were evaluated.

Results: Vaccination was completed in 191 subjects. Forty-four adverse reactions occurred within 28 days, most commonly mild pain and redness at the injection site or slight fatigue. At days 14 and 28, the seroconversion rates were 87.5% and 79.2% (50 EU), 100% and 95.8% (100 EU), and 95.8% and 87.5% (150 EU), respectively, with geometric mean titers (GMTs) of 18.1 and 10.6, 54.5 and 15.4, and 37.1 and 18.5, respectively, for the schedules with 2-week and 4-week intervals. Seroconversion was associated with synchronous upregulation of antibodies against the S protein, N protein and virion and a cytotoxic T lymphocyte (CTL) response. No cytokines and immune cells related to immunopathology were observed. Transcriptome analysis revealed the genetic diversity of immune responses induced by the vaccine.

Interpretation: In a population aged 18-59 years in this trial, this inactivated SARS-CoV-2 vaccine was safe and immunogenic.

Trial Registration: CTR20200943 and NCT04412538.
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http://dx.doi.org/10.1016/j.vaccine.2021.04.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040531PMC
May 2021

The role of multiple SARS-CoV-2 viral antigens in a vaccine-induced integrated immune response.

Vaccine 2021 04 22;39(18):2500-2503. Epub 2021 Mar 22.

Institute of Medical Biology, Chinese Academy of Medicine Sciences & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development for Severe Infectious Diseases, Kunming 650118, China. Electronic address:

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http://dx.doi.org/10.1016/j.vaccine.2021.03.067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983451PMC
April 2021

Targeting regulation of the tumour microenvironment induces apoptosis of breast cancer cells by an affinity hemoperfusion adsorbent.

Artif Cells Nanomed Biotechnol 2021 Dec;49(1):325-334

Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China.

The cytokine network of tumour microenvironment (TME) plays an important role in cancer growth and progression. The current work aims to provide a new strategy for cancer therapy based on the targeted regulation of cytokines in the TME. Here, heparin-coupled polyvinyl alcohol (PVA-H) microspheres have been developed as an adsorbent for selectively remove tumour-induced immunosuppressive cytokines, such as vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-β), but not tumour necrosis factor-alpha (TNF-α) which has an immune-stimulating effect and can inhibit tumour growth. The proliferation and apoptosis of breast cancer cells after perfusion were tested by cell viability assays, flow cytometry analysis and mRNA microarray assays. Results showed that the PVA-H microspheres efficiently absorbed the majority of VEGF (74.39%) and TGF-β (86.39%), but much less TNF-α (4.16%). The regulation of the cytokines had remarkable anti-proliferative and pro-apoptotic effects on breast cancer cells, which was further confirmed from the change of mRNA expression levels. Thus, targeting regulatory pathways within the TME by an affinity adsorbent that selectively depletes immunosuppressive cytokines is potentially a new and promising strategy for cancer therapy.
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http://dx.doi.org/10.1080/21691401.2021.1902337DOI Listing
December 2021

Effects of precipitation change and nitrogen addition on the composition, diversity, and molecular ecological network of soil bacterial communities in a desert steppe.

PLoS One 2021 17;16(3):e0248194. Epub 2021 Mar 17.

College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, China.

Currently, the impact of changes in precipitation and increased nitrogen(N) deposition on ecosystems has become a global problem. In this study, we conducted a 8-year field experiment to evaluate the effects of interaction between N deposition and precipitation change on soil bacterial communities in a desert steppe using high-throughput sequencing technology. The results revealed that soil bacterial communities were sensitive to precipitation addition but were highly tolerant to precipitation reduction. Reduced precipitation enhanced the competitive interactions of soil bacteria and made the ecological network more stable. Nitrogen addition weakened the effect of water addition in terms of soil bacterial diversity and community stability, and did not have an interactive influence. Moreover, decreased precipitation and increased N deposition did not have a superimposed effect on soil bacterial communities in the desert steppe. Soil pH, moisture content, and NH4+-N and total carbon were significantly related to the structure of bacterial communities in the desert steppe. Based on network analysis and relative abundance, we identified Actinobacteria, Proteobacteria, Acidobacteria and Cyanobacteria members as the most important keystone bacteria that responded to precipitation changes and N deposition in the soil of the desert steppe. In summary, we comprehensively analyzed the responses of the soil bacterial community to precipitation changes and N deposition in a desert steppe, which provides a model for studying the effects of ecological factors on bacterial communities worldwide.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0248194PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968660PMC
October 2021

Association of Mid- to Late-Life Blood Pressure Patterns With Risk of Subsequent Coronary Heart Disease and Death.

Front Cardiovasc Med 2021 15;8:632514. Epub 2021 Feb 15.

Department of Cardiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

The elevated blood pressure (BP) at midlife or late-life is associated with cardiovascular disease and death. However, there is limited research on the association between the BP patterns from middle to old age and incident coronary heart disease (CHD) and death. A cohort of the Atherosclerosis Risk in Communities (ARIC) Study enrolled 9,829 participants who attended five in-person visits from 1987 to 2013. We determined the association of mid- to late-life BP patterns with incident CHD and all-cause mortality using multivariable-adjusted Cox proportional hazards models. During a median of 16.7 years of follow-up, 3,134 deaths and 1,060 CHD events occurred. Compared with participants with midlife normotension, the adjusted hazard ratio for all-cause mortality and CHD was 1.14 (95% CI, 1.04-1.25) and 1.28 (95% CI, 1.10-1.50) in those with midlife hypertension, respectively. In further analyses, compared with a pattern of sustained normotension from mid- to late-life, there was no significant difference for the risk of incident death (HR, 1.15; 95% CI, 0.96-1.37) and CHD (HR, 1.33; 95% CI, 0.99-1.80) in participants with a pattern of midlife normotension and late-life hypertension with effective BP control. A higher risks of death and CHD were found in those with pattern of mid- to late-life hypertension with effective BP control (all-cause mortality: HR, 1.24; 95% CI, 1.08-1.43; CHD: HR, 1.65; 95% CI 1.30-2.09), pattern of midlife normotension and late-life hypertension with poor BP control (all-cause mortality: HR, 1.27; 95% CI, 1.12-1.44; CHD: HR, 1.53; 95% CI, 1.23-1.92), and pattern of mid- to late-life hypertension with poor BP control (all-cause mortality: HR, 1.49; 95% CI, 1.30-1.71; CHD: HR, 1.87; 95% CI, 1.48-2.37). The current findings underscore that the management of elderly hypertensive patients should not merely focus on the current BP status, but the middle-aged BP status. To achieve optimal reductions in the risk of CHD and death, it may be necessary to prevent, diagnose, and manage of hypertension throughout middle age.
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http://dx.doi.org/10.3389/fcvm.2021.632514DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7917074PMC
February 2021

Cathepsin B aggravates acute pancreatitis by activating the NLRP3 inflammasome and promoting the caspase-1-induced pyroptosis.

Int Immunopharmacol 2021 May 24;94:107496. Epub 2021 Feb 24.

Department of Intensive Care Unit, the Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China. Electronic address:

Objectives: Cathepsin B (CTSB), nod-like receptor family pyrin domain-containing 3 (NLRP3), and caspase-1 play an important role in the development of Acute Pancreatitis (AP). Besides, the relationship between the proteins remains poorly understood. In addition, whereas previous studies have found caspase-1 activation in AP, pyroptosis, a caspase-1 induced cell death mode, has never been proposed and proved in AP.

Methods: We induced AP in mice by intraperitoneal injection of cerulein. Mice in the inhibitor group of CTSB were pretreated with injection of CA-074me, while mice in the inhibitor group of caspase-1 were of Ac-YVAD-CHO, 1 h earlier. We evaluated the inflammation of the pancreas and the detected expression of activated CTSB, NLRP3, ASC, caspase-1p20, IL-1β and IL-18. TUNEL staining was used to detect acinar cell death.

Results: The inflammation of the pancreas in the two inhibitor groups was significantly reduced compared with that in the AP group. We observed that CA-074me not only inhibits CTSB, but also suppresses the expression and activity of NLRP3, ASC and caspase-1. We found that CA-074me further inhibits the downstream event of caspase-1, including pro-inflammatory cytokine secretion and pyroptosis. Whereas Ac-YVAD-CHO inhibited caspase-1 and decreased pro-inflammatory cytokine secretion and pyroptosis, it did not down-regulate the expression and activity ofCTSB, NLRP3 and ASC.

Conclusion: The results indicate that CTSB may aggravate AP by activating the NLRP3 inflammasome and promoting Caspase-1-induced pyroptosis. These provide clues about the pathophysiological mechanisms of AP, shedding light on new ideas and potential targets for the prevention and treatment of AP.
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http://dx.doi.org/10.1016/j.intimp.2021.107496DOI Listing
May 2021

The effect of comfort care based on the collaborative care model on the compliance and self-care ability of patients with coronary heart disease.

Ann Palliat Med 2021 Jan;10(1):501-508

Department of Cardiology, Taizhou People's Hospital, Taizhou, China. Email:

Background: Coronary heart disease (CHD) causes mental discomfort in patients, so there is a pressing need to strengthen the nursing cooperation with patients during treatment, which can help patients to regulate their psychological status, promoting successful rehabilitation. This study was to explore the effect of comfort care based on the collaborative care model (CCM) on the compliance and self-care ability of patients with CHD.

Methods: A total of 104 CHD patients were randomly selected in our hospital between April 2019 and April 2020. They were divided into two groups (with 52 cases in each group) using a random number table. Routine care was applied in the control group, while comfort care based on the CCM was employed in the study group. Self-made questionnaires were used to investigate the health knowledge proficiency, psychological status, compliance, self-care ability, and comfort degree in the two groups before and after intervention.

Results: Following the interventions, the health knowledge proficiency in the study group was higher than that in the control group (P<0.05). The Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS) scores in the study group were lower than those in the control group (P<0.05). The compliance rate in the study group was 96.15%, which was higher than that of the control group (82.69%) (P<0.05). The Exercise of Self-care Agency (ESCA) score in the study group was also higher than that of the control group (P<0.05). Moreover, the physical, mental, social, and emotional comfort scores in the study group were higher than those in the control group (P<0.05).

Conclusions: Comfort nursing based on the CCM improves CHD patients' health knowledge, regulates their psychological status, and improves their compliance, self-care ability, and comfort.
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http://dx.doi.org/10.21037/apm-20-2520DOI Listing
January 2021

Silence Is Golden.

Int Heart J 2021 ;62(1):178-180

Department of Cardiology, The First Affiliated Hospital, Sun Yet-sen University.

Speech-induced atrial tachycardia (AT) is extremely rare. We presented a case of focal AT that could be triggered by speech and terminated with the cessation of conversation. An electrophysiological study showed that the outbreak was associated with left atrial pressure rose. Radiofrequency ablation at the left atrial posterior-superior wall (earliest activation site) resulted in the immediate termination of AT. These electrophysiological characteristics indicated that the cardiac autonomic nervous system and/or left atrial pressure might play essential roles in the occurrences of speech-induced AT.
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http://dx.doi.org/10.1536/ihj.19-582DOI Listing
February 2021

Functionalized Carbon Nanotube-Embedded Poly(vinyl alcohol) Microspheres for Efficient Removal of Tumor Necrosis Factor-α.

ACS Biomater Sci Eng 2020 08 2;6(8):4722-4730. Epub 2020 Jul 2.

School of Ophthalmology & Optometry, Eye Hospital, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P. R. China.

Tumor necrosis factor (TNF)-α has an important role in the pathogenesis of autoimmune and inflammatory diseases such as rheumatoid and septic arthritis. Removal of excess tumor necrosis factor-α (TNF-α) is a promising treatment. In this study, a series of functionalized carbon nanotube-embedded poly(vinyl alcohol) (PVA) nanocomposite adsorbents were prepared for TNF-α removal for the first time. The resulting nanocomposites were characterized by scanning electron microscopy and Raman spectroscopy, which demonstrated that carbon nanotubes were well-dispersed on the surface of PVA macroporous microspheres. Adsorption tests showed that the carboxylated carbon nanotube-embedded composite microspheres (PVA/MWCNTs-COOH) possessed much better adsorption capacity for TNF-α in both simulated serum solution and rat plasma compared to the aminated (PVA/MWCNTs-NH) and raw carbon nanotube-embedded microspheres (PVA/MWCNTs-raw). In addition, the effects on hemolytic activity, the anticoagulant property, and the components of blood were negligible, indicating the excellent blood compatibility of composite beads. Our findings suggest that the carboxylated carbon nanotube-embedded composite microspheres may be potentially useful for the treatment of autoimmune and inflammatory diseases by removing TNF-α from the blood.
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http://dx.doi.org/10.1021/acsbiomaterials.9b01916DOI Listing
August 2020

Electrophysiological characteristics of epicardial to endocardial breakthrough in intractable cavotricuspid isthmus-dependent atrial flutter.

Pacing Clin Electrophysiol 2021 03 31;44(3):462-471. Epub 2021 Jan 31.

Department of Cardiology, Key Laboratory on Assisted Circulation, Ministry of Health, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, People's Republic of China.

Background: Epicardial to endocardial breakthrough (EEB) exists widely in atrial arrhythmia and is a cause for intractable cavotricuspid isthmus (CTI)-dependent atrial flutter (AFL). This study aimed to investigate the electrophysiological features of EEB in EEB-related CTI dependent AFL.

Methods: Six patients with EEB-related CTI-dependent AFL were identified among 142 consecutive patients who underwent CTI-dependent AFL catheter ablation with an ultra-high-density, high-resolution mapping system in three institutions. Activation maps and ablation procedure were analyzed.

Results: A total of seven EEBs were found in six patients. Four EEBs (including three at the right atrial septum and one in paraseptal isthmus) were recorded in three patients during tachycardia. The other three EEBs were identified at the inferolateral right atrium (RA) during pacing from the coronary sinus. The conduction characteristics through the EEB-mediated structures were evaluated in three patients. Two patients only showed unidirectional conduction. Activation maps indicated that CTI-dependent AFL with EEB at the atrial septum was actually bi-atrial macro-reentrant atrial tachycardia (BiAT). Intensive ablation at the central isthmus could block CTI bidirectionally in four cases. However, ablation targeted at the inferolateral RA EEB was required in two cases. Meanwhile, local potentials at the EEB location gradually split into two components with a change in activation sequence.

Conclusions: EEB is an underlying cause for intractable CTI-dependent AFL. EEB-mediated structure might show unidirectional conduction. CTI-dependent AFL with EEB at the atrial septum may represent BiAT. Intensive ablation targeting the central isthmus or EEB at the inferolateral RA could block the CTI bidirectionally.
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http://dx.doi.org/10.1111/pace.14164DOI Listing
March 2021

Efficacy of an inactivated bivalent vaccine for enterovirus 71 and coxsackievirus A16 in mice immunized intradermally.

Vaccine 2021 01 17;39(3):596-604. Epub 2020 Dec 17.

Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Kunming 650118, China. Electronic address:

Human hand, foot, and mouth disease (HFMD), an important infectious disease in children, is caused mainly by enterovirus 71 (EV71) and coxsackievirus A16 (CA16). In this study, a bivalent inactivated EV71/CA16 vaccine is developed and evaluated in immunized BALB/c mice injected through the intradermal route. Q-RT-PCR detection of the mRNA of immune signal molecules in local epithelial tissues inoculated with the vaccine indicates activation of innate immunity, which includes upregulation of immune-related chemokines, interferons and CD molecules. Further, the finding that neutralizing antibodies and specific T cellular responses were elicited in adult mice after two immunizations with the vaccine at a 28-day interval, which endowed offspring mice to defend a viral challenge, suggests the successful induction of specific protective antiviral immunity. All these data suggest that immunization with this bivalent EV71/CA16 vaccine via the intradermal route elicits effective immunity against EV71 and CA16 infection.
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http://dx.doi.org/10.1016/j.vaccine.2020.11.070DOI Listing
January 2021

Out-of-hospital cardiac arrest patients with implantable cardioverter-defibrillators: What are their outcomes?

Resuscitation 2020 12 22;157:141-148. Epub 2020 Oct 22.

University of Texas, Southwestern Medical Center, 5323 Harry Hines BLVD, Dallas, TX 75390-8579, USA.

The Aim Of The Study: To identify the prognostic factors and effects of implantable cardioverter-defibrillators (ICDs) in out-of-hospital cardiac arrest (OHCA) patients with ICDs because the clinical characteristics and outcomes of OHCA patients with ICDs are unknown.

Methods: The North American Resuscitation Outcomes Consortium (ROC) Cardiac Epistry Version 3 dataset was analyzed. Eligible patients were divided into OHCA patients with and without ICDs. Multivariable regressions were employed to analyze.

Results: Of 51,634 eligible OHCA patients, 581 (1.13%) had implanted ICDs. Among them, 53 (9.1%) patients survived to hospital discharge, and 40 (6.9%) patients had favorable neurological outcome at hospital discharge. Multivariable regression showed ICDs were not associated with OHCA outcomes in the total OHCA patients. In the OHCA patients with ICDs, shockable initial emergency medical services (EMS)-recorded rhythms and the ICD-shock-only defibrillation pattern were independent favorable factors for survival to hospital discharge(OR = 3.3, 95%CI 1.7-6.2, P < 0.001; OR = 2.4, 95%CI 1.1-5.5, P = 0.035, respectively) and neurological outcome at hospital discharge (OR = 6.5, 95%CI 2.9-14.4, P < 0.001; OR = 3.6, 95%CI 1.4-9.1, P = 0.006, respectively). During field resuscitation in OHCA patients with ICDs, at least 34.9% of total patients and 64.6% of patients with initial EMS-recorded VT/VF rhythms needed additional external shocks.

Conclusions: Shockable initial EMS-recorded rhythms and ICD-shock-only defibrillation pattern were independent factors for the favorable outcomes of OHCA patients with ICDs. ICDs were not associated with the outcomes of OHCA, and additional external shocks were needed in a substantial number of OHCA patients with ICDs during field resuscitation.
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http://dx.doi.org/10.1016/j.resuscitation.2020.10.016DOI Listing
December 2020

Virulence and pathogenesis of SARS-CoV-2 infection in rhesus macaques: A nonhuman primate model of COVID-19 progression.

PLoS Pathog 2020 11 12;16(11):e1008949. Epub 2020 Nov 12.

Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, Yunnan Province, People's Republic of China.

The COVID-19 has emerged as an epidemic, causing severe pneumonia with a high infection rate globally. To better understand the pathogenesis caused by SARS-CoV-2, we developed a rhesus macaque model to mimic natural infection via the nasal route, resulting in the SARS-CoV-2 virus shedding in the nose and stool up to 27 days. Importantly, we observed the pathological progression of marked interstitial pneumonia in the infected animals on 5-7 dpi, with virus dissemination widely occurring in the lower respiratory tract and lymph nodes, and viral RNA was consistently detected from 5 to 21 dpi. During the infection period, the kinetics response of T cells was revealed to contribute to COVID-19 progression. Our findings implied that the antiviral response of T cells was suppressed after 3 days post infection, which might be related to increases in the Treg cell population in PBMCs. Moreover, two waves of the enhanced production of cytokines (TGF-α, IL-4, IL-6, GM-CSF, IL-10, IL-15, IL-1β), chemokines (MCP-1/CCL2, IL-8/CXCL8, and MIP-1β/CCL4) were detected in lung tissue. Our data collected from this model suggested that T cell response and cytokine/chemokine changes in lung should be considered as evaluation parameters for COVID-19 treatment and vaccine development, besides of observation of virus shedding and pathological analysis.
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http://dx.doi.org/10.1371/journal.ppat.1008949DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660522PMC
November 2020

Randomized, double-blinded and placebo-controlled phase II trial of an inactivated SARS-CoV-2 vaccine in healthy adults.

Clin Infect Dis 2020 Nov 9. Epub 2020 Nov 9.

Institute of Medical Biology, Chinese Academy of Medicine Science & Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research and Development for Severe Infectious Diseases, Kunming, China.

Background: We evaluated an inactivated SARS-CoV-2 vaccine for immunogenicity and safety in adults aged 18-59 years.

Methods: In this randomized, double-blinded and controlled trial, healthy adults received a medium (MD) or a high dose (HD) of the vaccine at an interval of either 14 days or 28 days. Neutralizing antibody (NAb) and anti-S and anti-N antibodies were detected at different times, and adverse reactions were monitored for 28 days after full immunization.

Results: A total of 742 adults were enrolled in the immunogenicity and safety analysis. Among subjects in the 0, 14 procedure, the seroconversion rates of NAb in MD and HD groups were 89% and 96% with GMTs of 23 and 30, respectively, at day 14 and 92% and 96% with GMTs of 19 and 21, respectively at day 28 after immunization. Anti-S antibodies had GMTs of 1883 and 2370 in MD and 2295 and 2432 in HD group. Anti-N antibodies had GMTs of 387 and 434 in MD group and 342 and 380 in HD group. Among subjects in the 0, 28 procedure, seroconversion rates for NAb at both doses were both 95% with GMTs of 19 at day 28 after immunization. Anti-S antibodies had GMTs of 937 and 929 for MD and HD group, and anti-N antibodies had GMTs of 570 and 494 for MD and HD group, respectively. No serious adverse events were observed during the study period.

Conclusion: Adults vaccinated with inactivated SARS-CoV-2 vaccine had NAb as well as anti-S/N antibody, and had a low rate of adverse reactions.

Clinical Trials Registration: NCT04412538.
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http://dx.doi.org/10.1093/cid/ciaa1703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7717222PMC
November 2020

The association between SNPs and hepatitis B virus related acute-on-chronic liver failure.

Infect Genet Evol 2020 12 3;86:104615. Epub 2020 Nov 3.

Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China; Division of Infectious Diseases, State Key Laboratory of Biotherapy, Sichuan University, Chengdu, China. Electronic address:

Objective: This study intended to investigate the association between ten single nucleotide polymorphisms (rs1143623, rs12692386, rs1799983, rs2297518, rs2910164, rs3129859, rs4251961, rs4846085, rs641738, rs873457) with susceptibility and prognosis of hepatitis B related acute-on-chronic liver failure (HBV-ACLF).

Methods: This is a hospital-based case-control study included 274 patients with HBV-ACLF and 534 patients with chronic hepatitis B. The patients who were successfully followed were divided into the survival group and the death group according to the clinical outcome during the hospitalization and 90 days after discharge. The ten SNPs were genotyped in all subjects by using imLDR. Genotype, allele frequency, dominant model, recessive model and codominant model were constructed to investigate the association between single nucleotide polymorphisms with susceptibility and prognosis of HBV-ACLF.

Results: The genotype distribution of rs1143623 was statistically different between the two groups (P = 0.04), but the allele frequency was not statistically significant (P = 0.44). GC and GG + CG genotypes at rs1143623 reduced the risk of HBV-ACLF. There were only two GG and GT genotypes in rs1799983 in our study, and the genotype and allele frequency were statistically different between the death group and the survival group (P = 0.027, P = 0.023). Patients with T allele may reduce the risk of death in patients with HBV-ACLF. The genotype and allele frequency of rs2297518 showed no significant difference. In dominant models, patients with GA + AA genotypes at rs2297518 had a reduced risk of death.
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http://dx.doi.org/10.1016/j.meegid.2020.104615DOI Listing
December 2020

The utility of MEWS for predicting the mortality in the elderly adults with COVID-19: a retrospective cohort study with comparison to other predictive clinical scores.

PeerJ 2020 28;8:e10018. Epub 2020 Sep 28.

Department of Critical Care Medicine, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China.

Background: Older adults have been reported to be a population with high-risk of death in the COVID-19 outbreak. Rapid detection of high-risk patients is crucial to reduce mortality in this population. The aim of this study was to evaluate the prognositc accuracy of the Modified Early Warning Score (MEWS) for in-hospital mortality in older adults with COVID-19.

Methods: A retrospective cohort study was conducted in Wuhan Hankou Hospital in China from 1 January 2020 to 29 February 2020. Receiver operating characteristic (ROC) analysis was used to evaluate the predictive value of MEWS, Acute Physiology and Chronic Health Evaluation II (APACHE II), Sequential Organ Function Assessment (SOFA), quick Sequential Organ Function Assessment (qSOFA), Pneumonia Severity Index (PSI), Combination of Confusion, Urea, Respiratory Rate, Blood Pressure, and Age ≥65 (CURB-65), and the Systemic Inflammatory Response Syndrome Criteria (SIRS) for in-hospital mortality. Logistic regression models were performed to detect the high-risk older adults with COVID-19.

Results: Among the 235 patients included in this study, 37 (15.74%) died and 131 (55.74%) were male, with an average age of 70.61 years (SD 8.02). ROC analysis suggested that the capacity of MEWS in predicting in-hospital mortality was as good as the APACHE II, SOFA, PSI and qSOFA (Difference in AUROC: MEWS vs. APACHE II, -0.025 (95% CI [-0.075 to 0.026]); MEWS vs. SOFA, -0.013 (95% CI [-0.049 to 0.024]); MEWS vs. PSI, -0.015 (95% CI [-0.065 to 0.035]); MEWS vs. qSOFA, 0.024 (95% CI [-0.029 to 0.076]), all > 0.05), but was significantly higher than SIRS and CURB-65 (Difference in AUROC: MEWS vs. SIRS, 0.218 (95% CI [0.156-0.279]); MEWS vs. CURB-65, 0.064 (95% CI [0.002-0.125]), all < 0.05). Logistic regression models implied that the male patients (≥75 years) had higher risk of death than the other older adults (estimated coefficients: 1.16, = 0.044). Our analysis further suggests that the cut-off points of the MEWS score for the male patients (≥75 years) subpopulation and the other elderly patients should be 2.5 and 3.5, respectively.

Conclusions: MEWS is an efficient tool for rapid assessment of elderly COVID-19 patients. MEWS has promising performance in predicting in-hospital mortality and identifying the high-risk group in elderly patients with COVID-19.
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http://dx.doi.org/10.7717/peerj.10018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7528814PMC
September 2020

Can drip irrigation under mulch be replaced with shallow-buried drip irrigation in spring maize production systems in semiarid areas of northern China?

J Sci Food Agric 2021 Mar 3;101(5):1926-1934. Epub 2020 Oct 3.

College of Resources and Environment, Jilin Agricultural University, Changchun, China.

Background: In recent years, shallow-buried drip irrigation in spring maize production has gradually replaced drip irrigation under plastic films and has become a common irrigation method in semiarid areas of northern China.

Result: Two years of field experiments were carried out in the semiarid area of western Jilin province to compare the two drip irrigation methods in the spring maize production system. The treatments included MW1 (drip irrigation under mulch + moderate irrigation amount), MW2 (drip irrigation under mulch + high irrigation amount), SM1 (shallow-buried drip irrigation + moderate irrigation amount), and SM2 (shallow-buried drip irrigation + high irrigation amount). The maize yields were significantly higher under mulch than under shallow-buried drip irrigation, but there was no interaction between mulch and the irrigation amount. Drip irrigation under mulch greatly improved spring maize N, P and K uptake compared with that under shallow-buried drip irrigation. The agronomic-use efficiency, recovery-use efficiency, and partial factor productivity were ranked in the order of MW2 > MW1 > SW2 > SW1. The water-use efficiency of drip irrigation under mulch was 7.44% and 6.82% higher than that of shallow-buried drip irrigation under the moderate and high irrigation levels, respectively. However, considering the costs of the plastic mulch, there was no significant (P < 0.05) difference in economic benefits between the SW and MW treatments.

Conclusion: Drip irrigation under plastic film provides greater advantages for production, but shallow-buried drip irrigation may be a suitable method for farmers until fertigation technology is further optimized and the problem of plastic film pollution is solved. © 2020 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.10808DOI Listing
March 2021
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