Publications by authors named "Libo Wang"

173 Publications

High-Performance Wearable Strain Sensor Based on MXene@Cotton Fabric with Network Structure.

Nanomaterials (Basel) 2021 Mar 31;11(4). Epub 2021 Mar 31.

Henan Key Laboratory of Materials on Deep-Earth Engineering, School of Materials Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, China.

Flexible and comfortable wearable electronics are as a second skin for humans as they can collect the physiology of humans and show great application in health and fitness monitoring. MXene TiCT have been used in flexible electronic devices for their unique properties such as high conductivity, excellent mechanical performance, flexibility, and good hydrophilicity, but less research has focused on MXene-based cotton fabric strain sensors. In this work, a high-performance wearable strain sensor composed of two-dimensional (2D) MXene d-TiCT nanomaterials and cotton fabric is reported. Cotton fabrics were selected as substrate as they are comfortable textiles. As the active material in the sensor, MXene d-TiCT exhibited an excellent conductivity and hydrophilicity and adhered well to the fabric fibers by electrostatic adsorption. The gauge factor of the MXene@cotton fabric strain sensor reached up to 4.11 within the strain range of 15%. Meanwhile, the sensor possessed high durability (>500 cycles) and a low strain detection limit of 0.3%. Finally, the encapsulated strain sensor was used to detect subtle or large body movements and exhibited a rapid response. This study shows that the MXene@cotton fabric strain sensor reported here have great potential for use in flexible, comfortable, and wearable devices for health monitoring and motion detection.
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http://dx.doi.org/10.3390/nano11040889DOI Listing
March 2021

The Identification and Validation of Two Heterogenous Subtypes and a Risk Signature Based on Ferroptosis in Hepatocellular Carcinoma.

Front Oncol 2021 2;11:619242. Epub 2021 Mar 2.

Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: Ferroptosis is essential for tumorigenesis and progression of hepatocellular carcinoma (HCC). The heterogeneity of ferroptosis and its relationship with tumor microenvironment (TME) have still remain elusive.

Methods: Based on 74 ferroptosis related genes (FRGs) and 3,933 HCC samples from 32 datasets, we comprehensively explored the heterogenous ferroptosis subtypes. The clinical significance, functional status, immune infiltration, immune escape mechanisms, and genomic alterations of different subtypes were further investigated.

Results: We identified and validated two heterogeneous ferroptosis subtypes: C1 was metabolismimmunity subtype and C2 was metabolismimmunity subtype. Compared to C2, C1 owned worse prognosis, and C1 tended to occur in the patients with clinical characteristics such as younger, female, advanced stage, higher grade, vascular invasion. C1 and C2 were more sensitive to immunotherapy and sorafenib, respectively. The immune escape mechanisms of C1 might be accumulating more immunosuppressive cells, inhibitory cytokines, and immune checkpoints, while C2 was mainly associated with inferior immunogenicity, defecting in antigen presentation, and lacking leukocytes. In addition, C1 was characterized by BAP1 mutation, MYC amplification, and SCD1 methylation, while C2 was characterized by the significant alterations in cell cycle and chromatin remodeling processes. We also constructed and validated a robust and promising signature termed ferroptosis related risk score (FRRS) for assessing prognosis and immunotherapy.

Conclusion: We identified and validated two heterogeneous ferroptosis subtypes and a reliable risk signature which used to assess prognosis and immunotherapy. Our results facilitated the understood of ferroptosis as well as clinical management and precise therapy of HCC.
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http://dx.doi.org/10.3389/fonc.2021.619242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961086PMC
March 2021

Characterization, antioxidant and immunomodulatory effects of selenized polysaccharides from dandelion roots.

Carbohydr Polym 2021 May 13;260:117796. Epub 2021 Feb 13.

College of Art and Science, Northeast Agricultural University, Harbin 150030, China.

The polysaccharide (DRP) was gained from dandelion roots by ultrasonic-assisted enzymatic extraction (UAEE) followed by two-step column purification. Then selenylation of DRP has been accomplished by HNO-NaSeO method. sDRP-1 and sDRP-2 with the selenium content of 170 ± 1.13 and 710 ± 4.00 μg/g were prepared for further structural characterization and bioactivity determination. DRP, sDRP-1, and sDRP-2 were composed of the same monosaccharides in different molar ratios, and the molecular weights of DRP, sDRP-1 and sDRP-2 were 8700, 7900, and 5600 Da, respectively. Fourier transform infrared (FT-IR) spectra confirmed that DRP, sDRP-1, and sDRP-2 possessed similar functional groups. The results of Congo red test, X-ray diffraction (XRD) and scanning electron microscopy (SEM) showed that DRP, sDRP-1, and sDRP-2 had no three helix structure, did not form single crystal, and all belonged to amorphous morphology. sDRP-1 and sDRP-2 possessed greater antioxidant activities in vitro than the native polysaccharide DRP. At the same time, the selenized polysaccharides showed better immunomodulatory ability and could be used as new-type immunoenhancer. The present conclusions provided theoretical basis for the new application of dandelion polysaccharides and the development of dandelion resources.
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http://dx.doi.org/10.1016/j.carbpol.2021.117796DOI Listing
May 2021

CT-US fusion imaging increases the feasibility of early ultrasound-guided percutaneous intervention of local drug therapy in pancreatic contusion and laceration.

Ann Transl Med 2021 Feb;9(4):307

Department of Emergency, First Medical Center, General Hospital of the PLA, Beijing, China.

Background: Multimodal fusion imaging (MMFI) was usually used to assist percutaneous procedures for difficult lesions, with most applications occurring with hepatic and prostatic interventions. This paper aimed to evaluate the precision and effectiveness of computed tomography-ultrasound (CT-US) fusion imaging (CUFI)-assisted US-guided percutaneous intervention (UGPI) in early local drug therapy for pancreatic contusion and laceration (PCL).

Methods: A total of 12 pigs with PCL were randomly divided into a CUFI-assisted UGPI (MU) group (n=6) and a single UGPI (SU) group (n=6). The MU group underwent CUFI-assisted UGPI of locally applied medical protein glue (1 mL) injection while the SU group received the same therapy using two-dimensional UGPI. The duration and accuracy of each procedure were observed in the 2 groups.

Results: In the MU group, the overall time of the procedure for locking the plane was 1.85±0.06 minutes. Less time was spent in the selection of the pathway and puncture site in the MU group compared with the SU group (6.56±0.42 7.61±0.44 minutes, P<0.01). The duration of puncturing and drug injection was also shorter in the MU group than in the SU group (3.41±0.30 4.20±0.20 minutes, P<0.01) and the MU group had a higher accuracy of medical protein glue injection than the SU group (100% 50%, P<0.05).

Conclusions: CUFI could increase the precision and effectiveness of early UGPI in the delivery of local drug therapy in PCL.
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http://dx.doi.org/10.21037/atm-20-4426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944287PMC
February 2021

The effect of vibrating positive expiratory pressure therapy on refractory pneumonia prognosis in children.

Transl Pediatr 2021 Feb;10(2):315-322

Departments of Pneumonology, Children's Hospital of Fudan University, Shanghai, China.

Background: It is well documented that vibration techniques and positive expiratory pressure (PEP) can improve airway clearance, however, few clinical trials have studied the efficacy of vibrating PEP therapy on refractory () pneumonia (RMPP) in children. This study aimed to explore using vibrating PEP therapy in children with RMPP.

Methods: Sixty participants in the remission stage of RMPP were recruited into this randomized study and divided into two groups. The Acapella group (n=30) used the Acapella Choice twice daily for 2 months to clear their airways. The control group (n=30) used traditional chest percussion or postural drainage to mediate sputum expectoration. The groups administered their respective treatments at home and were followed up weekly. Participants kept a record of their treatment and condition in a daily log. The primary outcome of this study was the resolution of chest images, and the secondary outcome was the sputum period.

Results: There was no significant difference in sex or age distribution between the two groups. There were no significant differences at baseline between the groups in the location of their lesions (P=0.11). After 2 months of treatment, there was a better resolution of chest images in the Acapella group (P=0.00) compared with the control group. Additionally, the mean sputum period was significantly shorter for the Acapella group than for the control group (Acapella: 7.97±1.54 days; control: 11.90±1.64 days; P=0.00).

Conclusions: Vibrating PEP therapy is an effective therapy for children with RMPP, both in airway clearance and the resolution of lung abnormalities.
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http://dx.doi.org/10.21037/tp-20-237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944183PMC
February 2021

The functional polymorphisms linked with interleukin-1β gene expression are associated with bipolar disorder.

Psychiatr Genet 2021 Apr;31(2):72-78

The Second People's Hospital of Yuxi City, Yuxi, Yunnan, China.

Background: Bipolar disorder (BD) is a severe psychiatric illness attributable to multifactorial risk components (e.g. environmental stimuli, neuroinflammation, etc.), and genetic variations affecting these risk components are considered pivotal predisposing factors. The interleukin-1β (IL-1β) gene and its protein product have been repeatedly highlighted in the pathogenesis of BD. As functional polymorphisms and haplotypes linked with IL-1β mRNA expression have been reported, whether they are correlated with the risk of developing BD remains to be tested.

Methods: To examine whether variations in the IL-1β gene locus confer genetic risk of BD, we recruited 930 BD patients and 912 healthy controls for the current study. All subjects were Han Chinese, and were age- and gender-matched. We tested seven functional single nucleotide polymorphisms (SNPs) spanning the IL-1β gene and one haplotype composed of three SNPs for their associations with risk of BD.

Results: We found that the functional SNPs in the promoter region of IL-1β gene were significantly associated with risk of BD. The haplotype analyses further supported the involvement of IL-1β promoter SNPs in BD. The BD risk SNPs in our study have been previously reported to predict higher IL-1β levels in the brain and peripheral blood, which is consistent with the clinical observation of elevated IL-1β levels in the lymphocytes or peripheral blood of patients with BD compared with healthy subjects.

Conclusion: Our results support the contention that IL-1β is likely a risk gene for BD, and further investigations on this gene may promote our understanding and clinical management of this illness.
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http://dx.doi.org/10.1097/YPG.0000000000000272DOI Listing
April 2021

Handheld Ultrasound Device Usage and Image Acquisition Ability Among Internal Medicine Trainees: A Randomized Trial.

J Grad Med Educ 2021 Feb 29;13(1):76-82. Epub 2021 Dec 29.

Background: There is insufficient knowledge about how personal access to handheld ultrasound devices (HUDs) improves trainee learning with point-of-care ultrasound (POCUS).

Objective: To assess whether HUDs, alongside a yearlong lecture series, improved trainee POCUS usage and ability to acquire images.

Methods: Internal medicine intern physicians (n = 47) at a single institution from 2017 to 2018 were randomized 1:1 to receive personal HUDs (n = 24) for patient care/self-directed learning vs no-HUDs (n = 23). All interns received a repeated lecture series on cardiac, thoracic, and abdominal POCUS. Main outcome measures included self-reported HUD usage rates and post-intervention assessment scores using the Rapid Assessment of Competency in Echocardiography (RACE) scale between HUD and no-HUD groups.

Results: HUD interns reported performing POCUS assessments on patients a mean 6.8 (SD 2.2) times per week vs 6.4 (SD 2.9) times per week in non-HUD arm ( = .66). There was no relationship between the number of self-reported examinations per week and a trainee's post-intervention RACE score (rho = 0.022, = .95). HUD interns did not have significantly higher post-intervention RACE scores (median HUD score 17.0 vs no-HUD score 17.8; = .72). Trainee confidence with cardiac POCUS did not correlate with RACE scores.

Conclusions: Personal HUDs without direct supervision did not increase the amount of POCUS usage or improve interns' acquisition abilities. Interns who reported performing more examinations per week did not have higher RACE scores. Improved HUD access and lectures without additional feedback may not improve POCUS mastery.
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http://dx.doi.org/10.4300/JGME-D-20-00355.1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901629PMC
February 2021

Characterization of selenized polysaccharides from Ribes nigrum L. and its inhibitory effects on α-amylase and α-glucosidase.

Carbohydr Polym 2021 May 3;259:117729. Epub 2021 Feb 3.

College of Art and Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China. Electronic address:

The polysaccharide from Ribes nigrum L. (RCP) was modified by nitric acid-sodium selenite method. After purification by Sepharose-6B, high purity native (PRCP) and three selenized polysaccharides (PRSPs) with different selenium contents were obtained. Compared with PRCP, PRSPs possessed the lower molecular weight, better water-solubility, physical stability and rheological properties. FT-IR and NMR spectra confirmed PRSPs had the characteristic absorption peaks of polysaccharides and the glycosidic bond types were not changed after selenylation modification, whereas the selenyl groups existing in PRSPs were mainly introduced at the C-6 position of sugar residue →4)-β-d-Manp-(1→. Moreover, PRSPs displayed obviously smoother and smaller flaky structure than PRCP, and their inhibitory effects on α-amylase and α-glucosidase also were greater than PRCP. PRSPs exhibited a reversible inhibition on two enzymes in competitive manner and quenched their fluorescence through the static quenching mechanism. The polysaccharide-enzyme complex was spontaneously formed mainly driven by the hydrophobic interaction and hydrogen bonding.
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http://dx.doi.org/10.1016/j.carbpol.2021.117729DOI Listing
May 2021

Ultrafine particulate air pollution and pediatric emergency-department visits for main respiratory diseases in Shanghai, China.

Sci Total Environ 2021 Feb 11;775:145777. Epub 2021 Feb 11.

Department of Respiratory Medicine, Children's Hospital of Fudan University, Shanghai 201102, China. Electronic address:

Background: Few studies have explored the short-term effects of ultrafine particles (UFPs, particles < 0.1 μm) air pollution on the exacerbations of pediatric respiratory diseases.

Objectives: We aimed to evaluate short-term association between UFP and emergency-department visits (EDVs) for main pediatric respiratory diseases.

Methods: We collected daily data on UFP and pediatric EDVs for main respiratory diseases [asthma, pneumonia, bronchitis and upper respiratory tract infections (URTI)] from 66 hospitals in Shanghai, China from 2016 to 2018. Generalized additive models combined with polynomial distributed lag models were applied to explore the associations between UFP level and pediatric EDVs for respiratory diseases. We fitted two-pollutant models with criteria air pollutants and performed stratified analyses by gender and age.

Results: UFP was associated with increased EDVs for all respiratory diseases in cumulative lags up to 2 d and 3 d. The greatest risk was found at cumulative lags (0-2 d) for all respiratory diseases. At cumulative lags (0-2 d), an interquartile range increase in concentrations of UFP (1800 particles/cm) was associated with relative risks of EDVs due to asthma [1.35, 95% confidence interval (CI): 1.14-1.59], pneumonia (1.20, 95% CI: 1.04-1.38), bronchitis (1.17, 95% CI: 1.03-1.33) and URTI (1.14, 95% CI: 1.02-1.28). These associations were almost unchanged when controlling for criteria air pollutants, and there was no threshold below which the associations were not present. There were stronger associations in children aged 0-13 years.

Conclusions: Short-term exposure to UFP may independently increase the risks of EDVs for asthma, pneumonia, bronchitis and URTI exacerbations among children.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145777DOI Listing
February 2021

99mTc-MDP 3-Phase Bone Imaging in 3 Cases of Pigmented Villonodular Synovitis.

Clin Nucl Med 2021 May;46(5):405-408

From the Department of Nuclear Medicine, The Second Hospital of Jilin University, Changchun, Jilin Province, China.

Abstract: Pigmented villonodular synovitis (PVNS) is a rare proliferative synovial benign disorder, which is characterized by villonodular hyperplasia of joints, tendon sheath, and synovium; invasion of adjacent tissue; and sometimes visible hemosiderin deposition. Studies regarding bone scan findings of PVNS were relatively limited. Here, we report our findings on 99mTc-MDP 3-phase bone scan with SPECT/CT images on delayed phase in 3 patients with joint PVNS.
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http://dx.doi.org/10.1097/RLU.0000000000003548DOI Listing
May 2021

TTN/OBSCN 'Double-Hit' predicts favourable prognosis, 'immune-hot' subtype and potentially better immunotherapeutic efficacy in colorectal cancer.

J Cell Mol Med 2021 Apr 23;25(7):3239-3251. Epub 2021 Feb 23.

Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Colorectal cancer (CRC) remains a leading cause of cancer-related deaths worldwide. Although treatment strategies for solid tumours have been revolutionized by immunotherapy, only a small subset of CRC patients benefit. Using two-independent cohorts, we found the common frequently mutated genes TTN and OBSCN had the significant correlation with higher tumour mutation burden (TMB) and favourable overall survival. TTN and OBSCN also displayed significant commutation phenomenon. Therefore, based on the status of TTN and OBSCN, we stratified patients into 'Double-WT' phenotype, 'Single-Hit' phenotype and 'Double-Hit' phenotype. Importantly, the 'Double-Hit' phenotype had favourable prognosis, low malignant events propensity, and highest TMB, immune cells infiltration abundance, POLE mutation rate, microsatellite instability ratio, as well as immune checkpoints expression compared with the other two phenotypes. These results indicated that the 'Double-Hit' phenotype suggested 'immune-hot' tumours and potentially better immunotherapeutic efficacy. Bioinformatic algorithm assessment of immunotherapy responses also confirmed this conclusion, and the 'Double-Hit' phenotype was found to be a better predictor of immunotherapy than PD-L1, PD-1, CTLA-4, TMB and microsatellite status. This study revealed CRC patients with TTN/OBSCN 'Double-Hit' was significantly associated favourable prognosis, 'immune-hot' subtype and potentially better immunotherapeutic efficacy.
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http://dx.doi.org/10.1111/jcmm.16393DOI Listing
April 2021

Hypoxia Molecular Characterization in Hepatocellular Carcinoma Identifies One Risk Signature and Two Nomograms for Clinical Management.

J Oncol 2021 20;2021:6664386. Epub 2021 Jan 20.

Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

Hypoxia is a universal feature in the tumor microenvironment (TME). Nonetheless, the heterogeneous hypoxia patterns of TME have still not been elucidated in hepatocellular carcinoma (HCC). Using consensus clustering algorithm and public datasets, we identified heterogeneous hypoxia subtypes. We also revealed the specific biological and clinical characteristics via bioinformatic methods. The principal component analysis algorithm was employed to develop a hypoxia-associated risk score (HARS). We identified the two hypoxia subtypes: low hypoxia pattern (C1) and high hypoxia pattern (C2). C1 was less sensitive to immunotherapy compared to C2, consistent with the lack of immune cells and immune checkpoints (ICPs) in C1, whereas C2 was the opposite. C2 displayed worse prognosis and higher sensitivity to obatoclax relative to C1, while C1 was more sensitive to sorafenib. The two subtypes also demonstrated subtype-specific genomic variations including mutation, copy number alteration, and methylation. Moreover, we developed and validated a risk signature: HARS, which had excellent performance for predicting prognosis and immunotherapy. We revealed two hypoxia subtypes with distinct biological and clinical characteristics in HCC, which enhanced the understanding of hypoxia pattern. The risk signature was a promising biomarker for predicting prognosis and immunotherapy.
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http://dx.doi.org/10.1155/2021/6664386DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7846409PMC
January 2021

Concentration distribution and assessment of heavy metals in surface sediments in the Zhoushan Islands coastal sea, East China Sea.

Mar Pollut Bull 2021 Mar 30;164:112096. Epub 2021 Jan 30.

Yancheng Teachers University, City and Planning Institute, Yancheng 224002, China. Electronic address:

In an effort to assess the potential contamination and determine the environmental risks associated with heavy metals, 51 surface sediment samples (0-4 cm) were collected in the Zhoushan Islands coastal sea of the East China Sea (ECS). The heavy metal concentrations, grain size and total organic carbon (TOC) contents of these surface sediments were comprehensively analyzed. The results showed that the order of the average concentrations of heavy metals is Zn > Cr > Cu > Pb > As > Cd > Hg. Zn, Cr, Cd and Hg exhibit similar distribution and which derived from a common source, however, Cu, Pb and As present different distribution. The metal enrichment factor (EF) and geoaccumulation index (I) values both suggest that Cu and Cd likely pose environmental risks. The results of this study could provide scientific data to authorities in charge of sustainable marine management.
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http://dx.doi.org/10.1016/j.marpolbul.2021.112096DOI Listing
March 2021

Genetic Interaction of and Polymorphisms with Risk of Epilepsy in a Chinese Population.

Pharmgenomics Pers Med 2021 14;14:77-86. Epub 2021 Jan 14.

No. 1 Department of Neurology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130031, People's Republic of China.

Purpose: Long non-coding RNA H19 was highly expressed in the latent period of epilepsy, contributing to apoptosis of hippocampal neurons by targeting let-7b. Transforming growth factor beta receptor 1 (), a target of let-7b, is located on the susceptibility locus for epilepsy. In this context, we investigated the association between tagSNPs in long non-coding RNA and transforming growth factor beta receptor 1 () rs6478974 and the risk of epilepsy.

Patients And Methods: The present study consisted of 302 patients with epilepsy and 612 age- and gender-matched controls. The polymorphisms were analyzed using a TaqMan allelic genotyping assay. and mRNA levels were determined using quantitative real-time polymerase chain reaction.

Results: The AT and TT genotypes emerged as a protective factor for the risk of epilepsy (AT vs AA: adjusted OR = 0.59, 95% CI: 0.39-0.89, = 0.01; TT vs AA: adjusted OR = 0.53, 95% CI: 0.35-0.80, = 0.002, respectively). The protective effect was also observed in recessive genetic model (adjusted OR = 0.56, 95% CI: 0.38-0.82, = 0.003). Individuals carrying the rs6478974 TT genotype had lower levels of mRNA. Moreover, the TCTAT and TCCAA haplotypes emerged as a risk factor for epilepsy and the rs3741219-rs2839698-rs6478974 was associated with an interactive effect on the risk of epilepsy.

Conclusion: The current study provides evidence of the rs6478974 TT genotype decreasing the susceptibility to epilepsy by reducing the levels of mRNA.
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http://dx.doi.org/10.2147/PGPM.S279664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814234PMC
January 2021

Two novel compounds from green walnut husks ( Maxim.).

Nat Prod Res 2020 Dec 21:1-9. Epub 2020 Dec 21.

Department of Medicinal Chemistry and Natural Medicine Chemistry, College of Pharmacy, Harbin Medical University, Harbin, PR China.

Phytochemical investigation on the ethanol extract of green walnut husks ( Maxim.) led to the isolation of two previously unknown compounds, including a macrolide compound (13)-8-oxo-(9, 11)-8-oxo-octadeca-9,11-dien-13-olide () and a diarylheptanoid compound 1-(3'-methoxy-4'-hydroxyphenyl)-7-(3″,4″-dimethoxyphenyl)heptan-3-one (), together with 19 known compounds. The structures of these 21 compounds were elucidated by extensive analyses of NMR and HR-MS data, and the basis of spectroscopic analysis.
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http://dx.doi.org/10.1080/14786419.2020.1860976DOI Listing
December 2020

Temperature changes between neighboring days and childhood asthma: a seasonal analysis in Shanghai, China.

Int J Biometeorol 2020 Nov 23. Epub 2020 Nov 23.

Department of Respiratory Medicine, Children's Hospital of Fudan University, No. 399 Wanyuan Road, Shanghai, 201102, China.

Few evidences are available about the impact of temperature variation on childhood asthma in different seasons. This study aimed to assess the influence of temperature changes between neighboring days (TCN) on the exacerbation of asthma among children. Daily outpatient visits for childhood asthma (DOVCA) were collected from 17 main hospitals in Shanghai, China, from 2016 to 2018. A quasi-Poisson regression combined with distributed lagged nonlinear models was employed to estimate the association between TCN and asthma visits in cool or warm seasons, after controlling for short- and long-term trends, day of week, holidays, daily mean temperature, daily mean relative humidity, and air pollutants. The TCN varied from - 9.6 to 6.7 °C. The relationship between TCN and DOVCA greatly varied by season. In warm seasons, positive TCN (temperature rise) was associated with higher risks of asthma outpatient visits and negative TCN (temperature drop) was associated with lower risks; the associations were present on lag 1 day and lasted for 2 weeks; the cumulative relative risk of childhood asthma over 0 to 14 days was 1.98 (95% confidence interval: 1.42, 2.76) and 0.31 (95% confidence intervals: 0.21, 0.44) comparing a TCN of 2.5 °C (5th percentile) and - 3.2 °C (95th percentile) with 0 °C, respectively. In cool seasons, neither negative nor positive TCN showed significant risks. In conclusion, temperature rise might increase the risk of childhood asthma exacerbation and temperature drop might decrease the risks in warm seasons. There were no statistically significant influences in cool seasons.
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http://dx.doi.org/10.1007/s00484-020-02057-xDOI Listing
November 2020

Randomized clinical trial: efficacy and tolerability of two different split dose of low-volume polyethylene glycol electrolytes for bowel preparation before colonoscopy in hospitalized children.

Pediatr Res 2020 Oct 26. Epub 2020 Oct 26.

Department of Pediatric Gastroenterology Unit, The First Hospital of Jilin University, Changchun, Jilin, China.

Background: Eighty milliliter per kilogram of polyethylene glycol (PEG) for bowel preparation (BP) has been recommended, but the amount of liquid orally without nasogastric intubation is difficult to achieve. This study is to compare the efficacy and tolerability of two different low-volume PEG electrolyte solutions for BP in children.

Methods: The randomized, double-blind, controlled trial enrolled 150 children aged 6-18 years undergoing colonoscopy in our center. Patients were randomly assigned to receive 60 ml/kg (PEG-ELS 60) or 40 ml/kg (PEG-ELS 40) of PEG electrolytes (PEG-ELS) 4000. The Boston Bowel Preparation Scale was used for bowel cleansing evaluation. Primary end point was overall colon cleansing. Tolerability was also evaluated.

Results: PEG-ELS 40 and PEG-ELS 60 had similar efficacy in bowel cleansing for both whole colon and various colonic segments. The proportions of patients experiencing any adverse symptoms, or those who were willing to have BP repeated if necessary were similar in both groups. More patients considered the BP solution easy to take and be satisfied with the preparation in PEG-ELS 40 than PEG-ELS 60.

Conclusions: Low volume of PEG-ELS for BP has good efficacy in bowel cleansing. PEG-ELS with 40 ml/kg volume was not inferior to that of 60 ml/kg.

Impact: PEG-ELS 40 and PEG-ELS 60 had similar efficacy in bowel cleansing for whole and various colonic segments. The proportions of patients experiencing any adverse symptoms, or those who were willing to have BP repeated if necessary were similar in both groups. More patients considered BP solution easy to take and be satisfied with the preparation in PEG-ELS 40 than PEG-ELS 60. This study showed that low-volume PEG-ELS monotherapy was effective in bowel cleansing and explored a possibly feasible BP method for pediatrics in China that PEG-ELS 40 was comparable to PEG-ELS 60 regimen.
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http://dx.doi.org/10.1038/s41390-020-01216-5DOI Listing
October 2020

Mental Health Status of Healthcare Workers in China for COVID-19 Epidemic.

Ann Glob Health 2020 10 6;86(1):128. Epub 2020 Oct 6.

The Sixth Affiliated Hospital of Kunming Medical University, CN.

Background: COVID-19 first appeared in China in December 2019, with a high rate of infectivity and morbidity, which brought tremendous psychological pressure to healthcare workers.

Purpose: To understand the psychological health status of healthcare workers during the COVID-19 outbreak and decline, and to provide a theoretical reference for the future establishment of a psychological crisis intervention system.

Methods: Healthcare workers were recruited using convenience sampling and snowball sampling methods, and the electronic version of the SCL-90 scale and a sociodemographic questionnaire were administered. In the pretest, a total of 5018 responses were collected; after six weeks, random sampling was performed. The SCL-90 and measures of other epidemic-related problems were administered, with 1570 responses received; then, the final data analysis was performed.

Results: After six weeks, the post-test GSI score; SCL-90 total score; and PST, PSDI, O-C, I-S, DEP, ANX, PHOB, PAR, PSY, and HOS scores were significantly lower than the corresponding pretest scores (p < 0.05). The results by occupational category showed that the scores of nursing staff decreased significantly for 12 indexes and that the scores of the doctors and other hospital staff also significantly decreased. There was a significant difference between the pretest (50.78 ± 28.18) and post-test (45.00 ± 28.49) scores for the degree of worry about the epidemic. Healthcare workers believed that the top three aspects of life affected by the epidemic were economic problems (816 people), interpersonal communication problems (731 people), and mental health (728 people).

Conclusion: Over the course of the epidemic, the item scores generally declined significantly. Therefore, during an outbreak period, attention should be paid to psychological crisis interventions for healthcare workers; problems caused by psychological pressure, and even other psychological conditions, can be significantly alleviated to reduce the probability of subsequent health problems.
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http://dx.doi.org/10.5334/aogh.3005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7546117PMC
October 2020

Synthesis and evaluation of antitumor activities of 4-selenopyrimidine derivatives.

Nucleosides Nucleotides Nucleic Acids 2021 19;40(1):96-116. Epub 2020 Oct 19.

Department of Medicinal Chemistry and Natural Medicine Chemistry, College of Pharmacy, Harbin Medical University, Harbin, PR China.

Pyrimidine antimetabolic agents are the essential drugs in treatment of various tumors. Novel synthesis and biological evaluation of the pyrimidine derivatives incorporating selenium element and amino acid carrier as potential antitumor agents have not been tried and studied. Based on the biological significance of pyrimidine structure, these two additional elemental fragments maybe enhance the antitumor effect and reduce toxic side effects of pyrimidine agents. The aim of this paper is to synthesis a series of 4-selenopyrimidine derivatives in order to find more potent lead compounds against cancer. In this study, 12 new 4-selenopyrimidine derivatives that are unstable in acidic solutions but very stable in alkaline and neutral solutions avoiding light were synthesized, and the antitumor activities on HepG2 cell lines of these compounds were evaluated by MTT assay. The results have shown that these compounds could reduce the proliferation of HepG2 cells in a dose-dependent fashion, and the inhibitory activity of compounds a6 was greater than that of positive control 5-fluorouracil (5-FU), the IC for a6 was 3.63 μM. In the comprehensive analysis of the structure-activity relationship, we could draw the antitumor effect of selenouracil derivatives is stronger than those of selenothymine derivatives. These results suggest that the substituent groups of selenium element and amino acid on the pyrimidine derivatives are vital for their antitumor activities on HepG2 cells.
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http://dx.doi.org/10.1080/15257770.2020.1833342DOI Listing
October 2020

Degradation models, structure, rheological properties and protective effects on erythrocyte hemolysis of the polysaccharides from Ribes nigrum L.

Int J Biol Macromol 2020 Dec 21;165(Pt A):738-746. Epub 2020 Sep 21.

College of Art and Science, Northeast Agricultural University, Harbin 150030, China. Electronic address:

The polysaccharides from blackcurrant (Ribes nigrum L.) fruits were degraded by ultrasonic irradiation. Results showed that viscosity-average molecular weight decreased with increasing ultrasonic time or power. The degradation was fitted to the second-order kinetics model and midpoint chain scission model. Gas chromatographic analysis demonstrated that the native polysaccharide and three degraded polysaccharides were composed of the same monosaccharides but in different ratios. Fourier transform infrared and nuclear magnetic resonance spectroscopic analyses revealed the presence of α-, β-pyranose rings and the same six sugar residues in the four blackcurrant polysaccharides. Compared to the native polysaccharide, three degraded polysaccharides displayed better rheological properties and stronger protective effects against erythrocyte hemolysis. Collectively, the results support the potential utility of blackcurrant polysaccharides as natural antioxidants.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.09.093DOI Listing
December 2020

Sleep-disordered breathing and genetic findings in children with Prader-Willi syndrome in China.

Ann Transl Med 2020 Aug;8(16):989

Department of Endocrinology and Inherited Metabolic Diseases, Children's Hospital of Fudan University, Shanghai, China.

Background: Sleep-related breathing disorders are common in individuals with Prader-Willi syndrome (PWS), and can include hypersomnolence and obstructive sleep apnea, as well as central sleep breathing abnormalities that are present from infancy. Here we describe the sleep-disordered breathing (SDB) and genetic findings in patients with PWS in China.

Methods: In all, 48 patients confirmed by genetic tests were enrolled, 32 were under 2 years of age and 16 were older children. There were 37 (77.1%) patients with paternal 15q11-13 deletions, 11 (22.9%) patients with maternal uniparental disomy (mUPD), and no patients with imprinting defect (ID).

Results: Compared with infants, a significantly higher proportion of older children with PWS were overweight or obese (15/16 children 4/32 infants) and children had a higher serum level of free thyroxine (FT4) (0.9±0.2 0.7±0.7) and thyroxine (T4) (9.0±2.5 7.5±1.7). Age was correlated significantly with body mass index (BMI), T4, and FT4 (r=0.626, P=0.000; r=0.426, respectively). Overall, 42 of 48 (87.5%) patients had sleep apnea on polysomnography (PSG). Infants, when compared with older children, were more likely to experience central sleep apnea (71.8% 25%). In infants, there were no significant differences in the prevalence of SDB between the deletion group and the mUPD group.

Conclusions: Being overweight or obese was more common in older children with PWS. Compared with infants, a higher proportion children were overweight or obese and had higher serum levels of FT4 and T4. The prevalence of SDB was high in those with PWS, and central sleep apnea was found to be prevalent in infants. The pattern of SDB in infants with PWS was not significantly associated with the genotypes.
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http://dx.doi.org/10.21037/atm-20-4475DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475489PMC
August 2020

Vertebrobasilar Dolichoectasia as a Rare Cause of Simultaneous Abducens and Vestibulocochlear Nerve Symptoms: A Case Report and Literature Review.

Int J Gen Med 2020 20;13:523-527. Epub 2020 Aug 20.

Department of Neurosurgery, China-Japan Union Hospital of Jilin University, Changchun, People's Republic of China.

Vertebrobasilar dolichoectasia (VBD) can lead to cranial nerve symptoms. However, multiple cranial nerve symptoms associated with VBD in one case remain extremely rare. We here present the case of a 33-year-old male with VBD diagnosed by multimodality imaging, who developed simultaneous abducens and vestibulocochlear nerve symptoms and subsequently improved after blood pressure control treatment. To our knowledge, this is the first report of such a vascular disorder resulting in simultaneous symptoms of the abducens and vestibulocochlear nerves. This study highlights that such a vascular anomaly should be considered when cranial nerve symptom is encountered, especially when multiple cranial nerves involved. Meanwhile, radiological evalurrrrrrrrrrrrrrrrrrration of such neurovascular conflict using three-dimensional constructive interference in steady-state imaging is recommended.
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http://dx.doi.org/10.2147/IJGM.S269649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445531PMC
August 2020

Assessment of heavy metal contamination in surface sediments in the western Taiwan Strait.

Mar Pollut Bull 2020 Oct 27;159:111492. Epub 2020 Jul 27.

The Key Laboratory of Gas Hydrate, Ministry of Land and Resources, Qingdao Institute of Marine Geology, Qingdao 266071, China; Laboratory for Marine Mineral Resources, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. Electronic address:

The concentrations of the heavy metals Cu, Pb, Zn, As, Cr, Co, and Ni of a total of 187 surface sediment samples collected from the western Taiwan Strait were analyzed. The distribution characteristics and degree of contamination of these elements were investigated. The mean concentrations of Cu, Pb, Zn, As, Cr, Co, and Ni in the surface sediments of the study area were 10.2 mg/kg, 18.3 mg/kg, 51.7 mg/kg, 7.5 mg/kg, 38.7 mg/kg, 8.0 mg/kg, and 16.5 mg/kg, respectively. The heavy metals in the study area were mainly from natural sources. The regional pollution load index (PLIzone) was 0.64, indicating that there was no contamination, and an area with a relatively high PLIzone was found in the northern part of the study area. Furthermore, the impacts of the rapid development of Fujian's marine economy on the marine environment in the past decade should be further compared and analyzed.
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http://dx.doi.org/10.1016/j.marpolbul.2020.111492DOI Listing
October 2020

Associations of short-term exposure to air pollution and emergency department visits for pediatric asthma in Shanghai, China.

Chemosphere 2021 Jan 6;263:127856. Epub 2020 Aug 6.

Department of Respiratory Medicine, Children's Hospital of Fudan University, Shanghai, 201102, China. Electronic address:

There is limited evidence regarding the relationship between air pollution and pediatric asthma in developing countries. This study aimed to investigate the association between short-term exposure to ambient air pollutants and pediatric asthma emergency department (ED) visits in Shanghai, China. We collected data on six criteria air pollutants (PM, PM, NO, SO, CO, and O) and daily ED visits for pediatric asthma patients from 66 hospitals in Shanghai from 2016 to 2018. The generalized additive model combined with polynomial distributed lag model was applied to explore the associations. We fitted two-pollutant models and stratified the analyses by sex, age, and season. In total, we identified 108,817 emergency department visits for pediatric asthma. A 10 μg/m increase in the concentrations of PM, NO, SO, and O was significantly associated with increased risks of pediatric asthma ED visits, with relative risk of pediatric asthma of 1.011 [95% confidence interval (CI): 1.002, 1.021], 1.030 (95%CI: 1.017, 1.043), 1.106 (95%CI: 1.041, 1.174), and 1.009 (95%CI: 1.001, 1.017), respectively. The associations of NO remained robust in the two-pollutant models. There were stronger associations for older children (6-18 years) and in warm seasons. The concentration-response curves for pediatric asthma and PM, NO, SO, and O were steeper at lower and moderate concentrations but became flatter at higher concentrations. This analysis provided evidence that short-term exposure to air pollutants (PM, NO, SO, and O) could increase the risk of asthma exacerbations among children, and health benefits would be gained from improved air quality.
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http://dx.doi.org/10.1016/j.chemosphere.2020.127856DOI Listing
January 2021

miR-429 inhibits osteosarcoma progression by targeting HOXA9 through suppressing Wnt/β-catenin signaling pathway.

Oncol Lett 2020 Sep 24;20(3):2447-2455. Epub 2020 Jun 24.

Department of Orthopedics, Hanting People's Hospital, Weifang, Shandong 261100, P.R. China.

Osteosarcoma (OS) is the most commonly diagnosed malignant cancer of bone that occurs in adolescents and children. Mounting number of studies have indicated that miRNAs are increasingly playing fundamental roles in OS development. Thus, the biological function of miR-429 in OS progression was explored. The results of RT-qPCR revealed that miR-429 was downregulated in OS tissues and OS cell lines (MG-63, U2OS, Saos-2) while homeobox A9 (HOXA9) was markedly increased. Moreover, HOXA9 was confirmed as a direct target of miR-429 by using luciferase reporter assay. It was identified that miR-429 exhibited a suppressive effect on OS progression while HOXA9 showed the oncogenic function in OS progression by using MTT and Transwell assays. More importantly, rescue assays manifested that HOXA9 can partially overturn the suppressive effect of miR-429 on OS. Overexpression of miR-429 inhibited the activation of Wnt/β-catenin signaling pathway. In conclusion, miR-429 suppressed OS progression by targeting HOXA9 through Wnt/β-catenin pathway.
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http://dx.doi.org/10.3892/ol.2020.11766DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399823PMC
September 2020

Automated, High-Throughput Infrared Spectroscopy for Secondary Structure Analysis of Protein Biopharmaceuticals.

J Pharm Sci 2020 10 3;109(10):3223-3230. Epub 2020 Aug 3.

Pfizer, Inc., BioTherapeutics Pharmaceutical Sciences, Analytical Research and Development, Andover, MA 01810, USA. Electronic address:

Protein higher order structure (HOS) is an important product quality attribute that governs the structure-function characteristics, safety, and efficacy of therapeutic proteins. Infrared (IR) spectroscopy has long been recognized as a powerful biophysical tool in determining protein secondary structure and monitoring the dynamic structural changes. Such biophysics analyses help establish process and product knowledge, understand the impact of upstream (cell culture) and downstream (purification) process conditions, create stable formulations, monitor product stability, and assess product comparability when process improvements are implemented (or establish biosimilarity to originator products). This paper provides an overview of a novel automated mid-IR spectroscopic technique called microfluidic modulation spectroscopy (MMS) for the characterization of protein secondary structure. The study demonstrates that MMS secondary structure analysis of therapeutic monoclonal antibodies (mAb) is comparable with a conventional Fourier transform infrared (FTIR) method. More importantly the study shows MMS exhibits higher sensitivity and repeatability for low concentration samples over FTIR, as well as provides automated operation and superior robustness with simplified data analysis, increasing the utility of the instrument in determination of mAb secondary structure. Therefore, we propose that the MMS method can be widely applied in characterization and comparability/biosimilarity studies for biopharmaceutical process and product development.
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http://dx.doi.org/10.1016/j.xphs.2020.07.030DOI Listing
October 2020

Diverse effects of rutin and quercetin on the pasting, rheological and structural properties of Tartary buckwheat starch.

Food Chem 2021 Jan 18;335:127556. Epub 2020 Jul 18.

Institute of Food and Nutrition Development, Ministry of Agriculture and Rural Affaris, Haidian, Beijing 100081, China. Electronic address:

We investigated the interactions of two main phenolics, rutin and quercetin, with starch, the primary component of Tartary buckwheat. The addition of rutin or quercetin significantly affected the structural and physicochemical properties of the starch, and rutin showed a stronger effect than quercetin, particularly at a dose of 6% (w/w). Rutin better enhanced the aggregation of starch pastes and gel formation than quercetin according to our pasting, rheological and thermal property analyses. A scanning electron microscopy analysis of its morphology showed that rutin was more easily dispersed in starchy matrix than quercetin and acted as rigid fillers for gels. The nuclear magnetic resonance results showed different binding sites due to the steric hindrance of the rutin disaccharide groups (rutinose). These findings provide fundamental information about applying rutin during the whole grain processing of Tartary buckwheat.
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http://dx.doi.org/10.1016/j.foodchem.2020.127556DOI Listing
January 2021

lnc-BAZ2B promotes M2 macrophage activation and inflammation in children with asthma through stabilizing BAZ2B pre-mRNA.

J Allergy Clin Immunol 2021 Mar 23;147(3):921-932.e9. Epub 2020 Jul 23.

Institute of Pediatrics, Children's Hospital of Fudan University, and the Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, Fudan University, Shanghai, China; National Health Commission Key Laboratory of Neonatal Diseases, Fudan University, Shanghai, China. Electronic address:

Background: Dysregulation of long noncoding RNAs (lncRNAs) is associated with a variety of human diseases; however, whether they have a role in childhood asthma is unknown.

Objective: We sought to determine the differential expression profiles of lncRNAs in PBMCs of children with asthma and the mechanisms underlying the effects of lncRNAs on the pathogenesis of asthma.

Methods: The differential expression profiles of lncRNAs were analyzed by transcriptome microarray. The effects and mechanisms by which lncRNAs influence macrophage activation were detected by real-time quantitative PCR, Western blot, RNase protection assay, and chromatin immunoprecipitation assay. The roles played by lncRNAs in asthma were tested in a cockroach allergen extract (CRE)-induced mouse model.

Results: We identified 719 lncRNAs that were differentially expressed in PBMCs of children with asthma, 502 of which were upregulated and 217 were downregulated. An lncRNA of unknown function, lnc-BAZ2B, was dominantly expressed in monocytes and significantly upregulated in children with asthma. lnc-BAZ2B promotes M2 macrophage activation by enhancing BAZ2B expression and exacerbated lung inflammation in an M2 macrophage-associated CRE-induced asthma model. Mechanistically, lnc-BAZ2B promoted the expression of its cis target gene BAZ2B by stabilizing its pre-mRNA. BAZ2B, a reader of H3K14ac modification, enhanced the transcription of IRF4 and promoted M2 macrophage activation. lnc-BAZ2B expression was correlated with that of BAZ2B in PBMCs from children with asthma. Baz2b knockdown could alleviate asthma severity in a CRE-induced asthma model.

Conclusion: lnc-BAZ2B promotes M2 macrophage activation and inflammation in children with asthma and may serve as a potential therapeutic and diagnostic target in children with asthma.
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http://dx.doi.org/10.1016/j.jaci.2020.06.034DOI Listing
March 2021

Characterization and inhibitory activities on α-amylase and α-glucosidase of the polysaccharide from blue honeysuckle berries.

Int J Biol Macromol 2020 Nov 30;163:414-422. Epub 2020 Jun 30.

College of Art and Science, Northeast Agricultural University, Harbin 150030, China; National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, 150030, China. Electronic address:

An acidic heteropolysaccharide HEP-2 was obtained from blue honeysuckle berry extracts after purification using Sepharose 6B and Sephadex G-200 column chromatography. The molecular weight of HEP-2, determined with high-performance gel permeation chromatography, was 3.01 × 10 Da. Fourier transform infrared spectroscopy, gas chromatography and nuclear magnetic resonance analyses revealed the presence of α-, β-pyranose ring and six sugar residues in HEP-2. Amorphous aggregates of HEP-2 with irregular shape and lacking the triple helical structure were detected using the Congo red test, scanning electron and atomic force microscopy, and X-ray diffraction studies. Rheological characterization showed typical shear-thinning behavior and viscoelastic property of the polysaccharide. HEP-2 exerted significant inhibitory effects on α-amylase and α-glucosidase and displayed competitive inhibition kinetics. The collective results support the potential utility of HEP-2 as a hypoglycemic agent for enzyme-targeted treatment of diabetes or functional food.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.06.267DOI Listing
November 2020

Clinical and Genetic Spectrum of Children with Primary Ciliary Dyskinesia in China.

J Pediatr 2020 10 2;225:157-165.e5. Epub 2020 Jun 2.

Respirology Department, Children's Hospital of Fudan University, Shanghai, P.R. China. Electronic address:

Objective: To report detailed knowledge about the clinical manifestations, ciliary phenotypes, genetic spectrum as well as phenotype/genotype correlation in primary ciliary dyskinesia (PCD) in Chinese children.

Study Design: We recruited 50 Chinese children with PCD. Extensive clinical assessments, nasal nitric oxide, high-speed video analysis, transmission electron microscopy, and genetic testing were performed to characterize the phenotypes and genotypes of these patients.

Results: Common clinical features included chronic wet cough (85.4%), laterality defects (70.0%), and neonatal respiratory distress (55.8%). A high prevalence of congenital abnormalities (30.2%, 13/43), observed in patients who underwent comprehensive examination for comorbidities, included thoracic deformity (11.6%, 5/43), congenital heart disease (9.3%, 4/43), and sensorineural deafness (2.3%, 1/43). For 24 children age >6 years, the mean predicted values of forced expiratory volume in 1 second were 87.2%. Bronchiectasis evident on high-resolution computed tomography was reported in 38.1% of patients (16/42). Biallelic mutations (81 total; 57 novel) were identified in 13 genes: DNAAF3, DNAAF1, DNAH5, DNAH11, CCDC39, CCDC40, CCDC114, CCDC103, HYDIN, CCNO, DNAI1, OFD1, and SPAG1. Overall, ciliary ultrastructural and beat pattern correlated well with the genotype. However, variable phenotypes were also observed in CCDC39 and DNAH5 mutant cilia.

Conclusions: This large PCD cohort in China broadens the clinical, ciliary phenotypes, and genetic characteristics of children with PCD. Our findings are roughly consistent with previous studies besides some peculiarities such as high prevalence of associated abnormalities.
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http://dx.doi.org/10.1016/j.jpeds.2020.05.052DOI Listing
October 2020