Publications by authors named "Liang Zhang"

2,740 Publications

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Study on natural convection heat transfer in a closed cavity with hot and cold tubes.

Sci Prog 2021 Apr-Jun;104(2):368504211020965

School of Mechatronic Engineering, Southwest Petroleum University, Chengdu, Sichuan, P.R. China.

Laminar natural convection with a pair of hot and cold tube in a closed cubic cavity is carried out. This configuration can be founded in performance of nuclear power plant containment passive residual heat removal system. The basic government aquations are sloved by means of finite volume method. The effect of number (10-10), shape of tube and spatial position on local and mean heat transfer characteristics is studied. It is found that the number increased when raising number. The number is higher when the shapes are circle and triangle. In adddition, it is founded that the heat transfer has a better effect when the cold tube locates above the hot tube among the five spatial positions. The results provides theoretical basis for performance of nuclear power plant containment passive residual heat removal system.
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http://dx.doi.org/10.1177/00368504211020965DOI Listing
June 2021

CaCO-coated PVA/BC-based composite for the simultaneous adsorption of Cu(II), Cd(II), Pb(II) in aqueous solution.

Carbohydr Polym 2021 Sep 19;267:118227. Epub 2021 May 19.

School of Chemistry and Chemical Engineering, Xi'an University of Architecture and Technology, Xi'an, Shaanxi 710055, China.

A polymer composite material comprising polyvinyl alcohol/bacterial cellulose/calcium carbonate (PVA/BC/CaCO) was prepared for enabling the selective adsorption of toxic heavy metal ions, such as Cd(II), Cu(II), and Pb(II) from solution. FT-IR, SEM and XRD analyses confirmed the successful incorporation of CaCO into the PVA-based polymer by chemical cross-linking with epichlorohydrin. The optimal pH for adsorption of the metal ions onto PVA/BC/CaCO was determined to be 6.0. The pseudo-first-order kinetics model was best-suited for fitting the adsorption kinetics data, and the Langmuir model was best-suited for fitting the thermodynamic adsorption data. The maximum adsorption capacities of PVA/BC/CaCO for Cu(II), Pb(II), and Cd(II) were found to be 57.1, 513.6, and 238.6 mg/g, respectively, at 40 °C. In addition, the adsorbent was found to be highly recyclable. Overall, PVA/BC/CaCO adsorbent has the applicable potential in the removal of heavy metal ions from contaminated solution.
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http://dx.doi.org/10.1016/j.carbpol.2021.118227DOI Listing
September 2021

A retrospective analysis of prognostic factors for 160 patients with stage III small cell lung cancer.

Ann Palliat Med 2021 Jun 2. Epub 2021 Jun 2.

Department of Medical Thoracic Oncology, Jilin Provincial Cancer Hospital, Changchun, China.

Background: In some patients with stage III small cell lung cancer (SCLC), it is found that the treatment mode of systemic chemotherapy followed by auxiliary radiotherapy is better than early radiotherapy, but there is no clear evidence-based medical explanation for this. This study was designed to retrospectively evaluate prognostic factors for patients with stage III SCLC and explore the best treatment mode for locally advanced SCLC.

Methods: A total of 160 patients with stage III SCLC who underwent chemotherapy or chest radiotherapy were enrolled in this study, including 103 patients at stage IIIA and 57 patients at stage IIIB. The short-term and long-term outcomes following chemotherapy and chest radiotherapy were compared between the two groups.

Results: There was no significant difference in progression-free survival (PFS) (9.5 vs. 10.0 months, P=0.065) or overall survival (OS) (14.0 vs. 14.0 months, P=0.231) between early radiotherapy and late radiotherapy in stage IIIA SCLC. PFS in stage IIIB patients was longer in the late radiotherapy group than in early radiotherapy (11.0 vs. 9.0 months, P=0.041), but the difference in OS was not statistically significant between the two groups (14.0 vs. 17.0 months, P=0.110). There was no significant difference in short-term and long-term therapeutic effects between stages IIIA and IIIB. Patients with stage IIIB who received late radiotherapy seemed to have a survival advantage, but the difference was not statistically significant (P=0.549).

Conclusions: Treatment mode had no impact on patients at stage IIIA. Late radiotherapy showed more effectiveness for patients at stage IIIB.
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http://dx.doi.org/10.21037/apm-21-50DOI Listing
June 2021

Metagenomic insights into the effect of thermal hydrolysis pre-treatment on microbial community of an anaerobic digestion system.

Sci Total Environ 2021 Jun 1;791:148096. Epub 2021 Jun 1.

Advanced Environmental Biotechnology Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, Singapore 637141, Singapore; School of Civil and Environmental Engineering, Nanyang Technological University, Singapore 639798, Singapore. Electronic address:

Thermal hydrolysis process (THP) is an effective pre-treatment method to reduce solids volume and improve biogas production during anaerobic digestion (AD) via increasing the biodegradability of waste activated sludge (WAS). However, the effects of THP pre-treated sludge on microbial diversity, interspecies interactions, and metabolism in AD systems remain largely unknown. We therefore setup and operated an anaerobic digester during a long-term period to shed light on the effect of THP pre-treatment on AD microbial ecology in comparison to conventional AD via Illumina based 16S rRNA gene amplicon sequencing and genome-centric metagenomics analysis. Results showed THP sludge significantly reduced the microbial diversity, shaped the microbial community structure, and resulted in more intense microbial interactions. Compared to WAS as the feed sludge, THP sludge shaped the core functional groups, but functional redundancy ensured the system's stability. The metabolic interactions between methanogens and syntrophic bacteria as well as the specific metabolic pathways were further elucidated. Hydrogenotrophic methanogens, Methanospirillum sp. and Methanolinea sp., were the primary contributors for methane production when treating THP and WAS, respectively, which also have potential for acetate oxidation to methane. Collectively, this study provides in-depth information on the interspecies interactions to better understand how THP pre-treatment influences AD microbial community.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148096DOI Listing
June 2021

Rheological behaviors and physicochemical changes of doughs reconstituted from potato starch with different sizes and gluten.

Food Res Int 2021 Jul 11;145:110397. Epub 2021 May 11.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, PR China. Electronic address:

The effects of different sizes of potato starch on the rheological and physiochemical properties of model doughs were investigated. Compared with those of model dough prepared from original starch, the strengths of model doughs prepared from fractionated starch were higher, which indicates that fractionated starch can positively influence the properties of doughs. Additionally, the model dough prepared using large size starch granules had higher storage modulus (G'), loss modulus (G''), and composite modulus (|G*|) values compared to those of other types of dough; it also had the highest elasticity, viscosity, and strength. This might be related to its high amylose content (20.28 ± 0.69%) and high 1045 cm/1022 cm ratio (1.27 ± 0.17). The model dough (S) prepared from starch with small sizes had the highest contents of disulfide bonds (2.91 μmolg), β-turn (33.92 ± 1.17%), and β-sheet (22.57 ± 0.54%); and it also had better network structure and dough stability. Thus, the stability of the S model dough was affected by phosphorus (1194.57 ± 25.32 ppm) and amylopectin (84.19 ± 1.88%) content, and, moreover, by the competition for water. Stability and network structure of dough are relative to the size distribution of starch granules. Finally, a schematic model showing the mechanism of the influence of phosphorus, sulfhydryl, and disulfide bonds in fractionated starch on the rheological properties of dough was developed.
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http://dx.doi.org/10.1016/j.foodres.2021.110397DOI Listing
July 2021

Preparation and bacteriostatic research of porous polyvinyl alcohol / biochar / nanosilver polymer gel for drinking water treatment.

Sci Rep 2021 Jun 9;11(1):12205. Epub 2021 Jun 9.

School of Chemistry and Chemical Engineering, Xi'an University of Architecture and Technology, Xi'an, 710055, Shaanxi, China.

Microbial contamination in drinking water has become an important threat to human health. There is thus an urgent need to develop antibacterial materials to treat drinking water. Here, porous silver-loaded biochar (C-Ag) was prepared using corn straw as the substrate and silver as the antibacterial agent. C-Ag was then uniformly distributed in polyvinyl alcohol gel beads of eluted calcium carbonate to prepare p-PVA/C-Ag antibacterial composite. The polymer composites were tested by FT-IR, XRD, SEM and TG-DSC. The results showed that C-Ag was more evenly distributed in the PVA gel spheres. Antibacterial experiments showed that p-PVA/C-Ag greatly inhibited Escherichia coli. Practical application tests revealed that p-PVA/C-Ag showed high and sustained bactericidal inhibition and reusability. Generally, p-PVA/C-Ag composite shows high potential to be applied to drinking water treatment.
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http://dx.doi.org/10.1038/s41598-021-91833-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190314PMC
June 2021

miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3.

Cell Rep 2021 Jun;35(10):109225

CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing 100101, China; Department of Plastic & Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China. Electronic address:

Maintaining a suitable level of sensitivity to environmental cues is crucial for proper function of adult stem cells. Here, we explore how the intrinsic sensitivity of skin hair follicle (HF) progenitors to growth stimuli is dynamically regulated. We discover miR-24 is an miRNA whose expression in HF progenitors inversely correlates with their growth potency in vivo. We show that its upregulation in adult skin epithelium leads to blunted responses of HF progenitors to growth cues and retards hair regeneration, while its conditional ablation leads to hyper-sensitized growth responsiveness of HF progenitors and precocious hair regeneration. Mechanistically, we find that miR-24 limits the intrinsic growth competence of HF progenitor by directly targeting Plk3, whose downregulation leads to reduced expression of CCNE1, a key cyclin for cell-cycle entry. These findings reveal an miRNA-mediated dynamic and cell-intrinsic mechanism used by HF progenitors to adapt their regenerative competence for different physiological conditions.
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http://dx.doi.org/10.1016/j.celrep.2021.109225DOI Listing
June 2021

Mechanism of Laccase Induction via Emodin in .

Front Bioeng Biotechnol 2021 18;9:653800. Epub 2021 May 18.

National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, China.

Secondary metabolites of traditional Chinese herbs can prominently stimulate the production of laccase from white rot fungi during submerged fermentation. However, the molecular mechanism through which these natural products induce the production of laccase remains unknown. In this study, the Chinese herbal medicine was used to induce laccase production in , and the best inducer was identified in emodin, even under conditions of 1000-L, large-scale fermentation. Proteomics analysis identified a selection of proteins that were differentially expressed in the presence of emodin, indicating that emodin may affect the expression of laccase genes through three mechanisms: reducing bioenergy productivity, the aryl hydrocarbon receptor (AHR)/xenobiotic response element (XRE) pathway, and the nuclear erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway. Combined with protoplast flow cytometry and fluorescence, it is revealed that emodin might reduce the synthesis of ATP by lowering the mitochondrial membrane potential, leading to the subsequent responses.
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http://dx.doi.org/10.3389/fbioe.2021.653800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171328PMC
May 2021

Visible light responsive, self-activated bionanocomposite films with sustained antimicrobial activity for food packaging.

Food Chem 2021 May 25;362:130201. Epub 2021 May 25.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address:

The research on a new type of low-cost, less-loss and adjustable sustained antibacterial activity food packaging films with self-activation ability and great industrialization potentiality is of great scientific and technological interest. Herein, a novel chitosan/negatively charged graphitic carbon nitride self-activation bionanocomposite films was prepared by one-step electrostatic self-assembly. First, the antibacterial efficiency of this film could reach to 99.8 ± 0.26% against E. coli and 99.9 ± 0.04% against S. aureus through self-activated under visible light. Second, this film can effectively extend the shelf life of tangerines to 24 days. Hemolysis and cell experiment test proved that this film was safe and nontoxic. Finally, negatively charged graphitic carbon nitride with low-cost can improve the mechanical, thermal and hydrophobic properties of neat chitosan films. This work can provide a new pathway for the preparation of low-cost packaging films with excellent visible light responsive property and sustainable antibacterial activity.
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http://dx.doi.org/10.1016/j.foodchem.2021.130201DOI Listing
May 2021

Patient with Lobular Carcinoma of the Breast and Activating AKT1 E17K Variant.

Acta Med Acad 2021 Apr;50(1):209-217

Herbert-Herman Cancer Center, E. W. Sparrow Hospital, Lansing, MI, USA.

Objective: To present the characteristics of the AKT1E117K gene variant and a description of the clinical application in a patient with metastatic breast cancer.

Results: 63 y/o woman with Stage IV Invasive lobular carcinoma at diagnosis was treated with Palbociclib and aromatase inhibitors (AI). At progression, tissue was sent for comprehensive genomic profiling to Foundation Medicine (FM) which revealed AKT1E17K mutation. In lieu of available clinical data within the patient's tumor type (HR+ HER2- breast cancer), extrapolated data from the Flatiron Health-FM (FH-FMI) Clinico-genomic Database (CGDB) was discussed at our Molecular Tumor Board (MTB). After multidisciplinary discussion, the consensus recommendation was to start treatment with the combination of mTOR inhibitor everolimus, and AI, exemestane. Patient tolerated treatment without major side effects. By the second clinical visit the patient's breast showed signs of improvement. PET/CT showed diminished left axillary uptake, decreased right paratracheal lymph node PET avidity, and stable bone disease consistent with a partial response. The most recent office visit in January 2021, breast exam revealed a normal-appearing skin with only faint erythema. All other skin lesions have resolved. Although, the role of AKT1 variant described here is not well defined and therapeutic significance of M-Tor inhibitors not established in metastatic breast cancers, comprehensive approach to this case unraveled new and successful therapeutic option in this patient.

Conclusion: This demonstrates that applying available Precision Medicine tools like MTB and real world data sets from patient populations with similar clinical and genomic profiles may provide more options for treatment.
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http://dx.doi.org/10.5644/ama2006-124.336DOI Listing
April 2021

Physicochemical and Structural Characterization of Potato Starch with Different Degrees of Gelatinization.

Foods 2021 May 17;10(5). Epub 2021 May 17.

Department of Food Science and Formulation, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, B-5030 Gembloux, Belgium.

Starch gelatinization has been widely studied previously, but there is still a lack of systematical research on the relationship between the degree of starch gelatinization (DSG) and its physicochemical and structural properties. In this study, potato starch samples with DSG ranging from 39.41% to 90.56% were obtained by hydrothermal treatment. The thermal, rheological, and structural properties, as well as the water-binding capacity of samples were investigated. A starch solution with a DSG of 39.41% was partially sedimented at room temperature, while starch with a DSG of 56.11% can form a stable paste with a fine shear-thinning property, as well as samples with a DSG larger than 56.11%. The endothermic enthalpy, gelatinization range, and short-range ordered structure of starch were negatively correlated with DSG, whereas onset gelatinization temperature, apparent viscosity, and water-binding capacity were positively correlated. The viscoelasticity of starch gels was negatively correlated with the DSG after full gelatinization (DSG > 39.41%). Starch granules gradually lose their typical shape and less birefringence can be observed with increasing DSG. Hydrothermal treatment has a more significant effect on the amount of exposed hydroxyl groups than the ordered and amorphous structures of partially gelatinized starch. This study built linear correlations between starch physicochemical properties and the DSG and provided comprehensive insight into the characteristics of partially gelatinized potato starch.
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http://dx.doi.org/10.3390/foods10051104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8156876PMC
May 2021

Preparation of Plastics- and Foaming Agent-Free and Porous Bamboo Charcoal based Composites Using Sodium Silicate as Adhesives.

Materials (Basel) 2021 May 11;14(10). Epub 2021 May 11.

School of Engineering, Zhejiang A&F University, Hangzhou 311300, China.

Plastics and foaming agents are often used to prepare large-size and low-density bamboo charcoal (BC) based composites. In this study, a plastic-free and foaming agent-free BC based composite was prepared by substituting sodium silicate (SS) for plastics. The effect of both the BC particle sizes and the usage amount of SS on the mechanical and adsorptive properties of the BC/SS composites were investigated. The experimental results show that when the BC particle size is 270 μm and the mass ratio of BC to SS is equal to 10:5, the BC/SS composite has the optimal foaming effect and best comprehensive properties. In addition, the foaming pores of the composite are caused by water vapor, which has difficulty escaping the BC because of the blockage of SS during the hot pressing process. In the BC/SS composite (10:5), the static bending intensity and the compressive strength reach respectively 6.13 MPa and 5.5 MPa, and the average pore size and porosity are 557.85 nm and 52.03%, respectively. In addition, its formaldehyde adsorptionrate reaches 21.6%. In view of good mechanical properties, formaldehyde adsorption, and environmentally friendly performance, the BC/SS composite has a great potential as a core layer of interior building materials.
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http://dx.doi.org/10.3390/ma14102468DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151041PMC
May 2021

VDAC1 regulates mitophagy in NLRP3 inflammasome activation in retinal capillary endothelial cells under high-glucose conditions.

Exp Eye Res 2021 May 29;209:108640. Epub 2021 May 29.

Guangdong Eye Institute, Department of Ophthalmology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China. Electronic address:

Diabetic retinopathy (DR) has been considered to involve mitochondrial alterations and be related to the nucleotide-binding oligomerization domain-like receptors 3 (NLRP3) inflammasome activation. The voltage-dependent anion channel 1 (VDAC1) protein is one of the key proteins that regulates the metabolic and energetic functions of the mitochondria. To explore the involvement of VDAC1 in mitophagy regulation of NLRP3 inflammasome activation under high-glucose (HG) conditions, this study examined expressions of VDAC1, mitochondrial function and mitophagy-related proteins, and NLRP3 inflammasome-related proteins in human retinal capillary endothelial cells (HRCECs) cultured with 30 mM of glucose in the presence or absence of mitophagy inhibitor (Mdivi-1) using Western blot. Mitochondrial membrane potential and mitochondrial reactive oxygen species (mtROS) were detected using flow cytometry. GFP-tagged pAdTrack-VDAC1 adenovirus was used to overexpress VDAC1. Cell biological behaviors, including proliferation, migration, tubule formation, and apoptosis, were also observed. Our results showed that when compared to the normal glucose and high mannitol groups, increased amounts of mitochondrial fragments, reduced mitochondrial membrane potential, increased expression of mitochondrial fission protein Drp 1, decreased expression of mitochondrial fusion protein Mfn 2, accumulation of mtROS, and activation of the NLRP3 inflammasome were observed in the HG group. Meanwhile, HG markedly reduced the protein expressions of PINK1, Parkin and VDAC1. Inhibition of mitophagy reduced PINK1 expression, enhanced NLRP3 expression, but failed to alter VDAC1. VDAC1 overexpression promoted PINK1 expression, inhibited NLRP3 activation and changed the cell biological behaviors under HG conditions. These findings demonstrate that VDAC1-mediated mitophagy plays a crucial role in regulating NLRP3 inflammasome activation in retinal capillary endothelial cells under HG conditions, suggesting that VDAC1 may be a potential target for preventing or treating DR.
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http://dx.doi.org/10.1016/j.exer.2021.108640DOI Listing
May 2021

Comparison of high- and low-viscosity cement percutaneous vertebroplasty with manipulative reduction for the treatment of Kümmell's disease: A retrospective cohort study.

J Back Musculoskelet Rehabil 2021 May 21. Epub 2021 May 21.

Objective: To compare the clinical outcomes and cement leakage rate between high-viscosity and low-viscosity cement percutaneous vertebroplasty (PVP) with manipulative reduction in the treatment of Kümmell's disease (KD).

Method: PVP using high-viscosity cement (Group H) or low-viscosity cement (Group L) with manipulative reduction was compared in 86 KD patients. Visual Analogue Scale (VAS), Oswestry Disability Index (ODI), vertebral height, kyphosis angle and complications were evaluated and compared preoperative, one day postoperative, and at final follow-up.

Results: The VAS score, ODI score, mean anterior and middle vertebral height variation significantly improved in both groups postoperatively. However, there was no significant difference between the two groups considering the above results preoperative and postoperative. Significantly less cement leakage (13.6%) and shorter operative time (35.6 ± 5.1 min) were observed in Group H when compared with Group L (45.2% and 41.1 ± 5.3 min) (p< 0.05).

Conclusion: The clinical outcomes and radiologic findings of PVP with manipulative reduction was not influenced by cement viscosity. However, high cement viscosity PVP was found to be significant less cement leakage and shorter operative time.
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http://dx.doi.org/10.3233/BMR-191778DOI Listing
May 2021

Individualized Nomogram for Predicting Survival in Patients with Brain Metastases After Stereotactic Radiosurgery Utilizing Driver Gene Mutations and Volumetric Surrogates.

Front Oncol 2021 13;11:659538. Epub 2021 May 13.

Department of Oncology, Guangdong Sanjiu Brain Hospital, Guangzhou, China.

It is well-known that genomic mutational analysis plays a significant role in patients with NSCLC for personalized treatment. Given the increasing use of stereotactic radiosurgery (SRS) for brain metastases (BM), there is an emerging need for more precise assessment of survival outcomes after SRS. Patients with BM and treated by SRS were eligible in this study. The primary endpoint was overall survival (OS). Cox regression models were used to identify independent prognostic factors. A survival predictive nomogram was developed and evaluated by Concordance-index (C-index), area under the curve (AUC), and calibration curve. From January 2016 to December 2019, a total of 356 BM patients were eligible. The median OS was 17.7 months [95% confidence interval (CI) 15.5-19.9] and the actual OS at 1- and 2-years measured 63.2 and 37.6%, respectively. A nomogram for OS was developed by incorporating four independent prognostic factors: Karnofsky Performance Score, cumulative tumor volume, gene mutation status, and serum lactate dehydrogenase. The nomogram was validated in a separate cohort and demonstrated good calibration and good discriminative ability (C-index = 0.780, AUC = 0.784). The prognostic accuracy of the nomogram (0.792) was considerably enhanced when compared with classical prognostic indices, including the Graded Prognostic Assessment (0.708), recursive partitioning analysis (0.587), and the SRS (0.536). Kaplan-Meier curves showed significant differences in OS among the stratified low-, median- and high-risk groups ( < 0.001). In conclusion, we developed and validated an individualized prognostic nomogram by integrating physiological, volumetric, clinical chemistry, and molecular biological surrogates. Although this nomogram should be validated by independent external study, it has a potential to facilitate more precise risk-stratifications to guide personalized treatment for BM.
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http://dx.doi.org/10.3389/fonc.2021.659538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8158152PMC
May 2021

In vivo imaging via a red-emitting fluorescent probe to diagnosing liver cancer or drug-induced liver disease.

Anal Chim Acta 2021 Jul 6;1168:338621. Epub 2021 May 6.

Department of Radiology, Xinqiao Hospital, Army Medical University, Chongqing, 400037, PR China. Electronic address:

Leucine aminopeptidase (LAP) as an important proteolytic enzyme, has been mainly found in hepatobiliary cells, and overexpressed in hepatoma cells. Herein, a new highly selective red-emitting fluorescent probe (DCDHF-Ala) for LAP has been synthesized based on 2-dicyanomethyldiene-3-cyano-2, 5-dihydrofuran (DCDHF) as fluorophore, and alanine (Ala) as the detection group. More importantly, it's the first time to use Ala as a reactive group for LAP. DCDHF-Ala has a low detection limit (0.20 U/L), excellent water solubility and cell membrane permeability. In addition, the probe has been successfully applied to fluorescent imaging in cells and zebrafish. It's especially worth mentioning that, DCDHF-Ala has a high biosafety and enables a real-time detection of LAP levels in mice model. What's the most important is that DCDHF-Ala may be an effective tool to qualitatively monitor the upregulation of LAP induced by liver injury and liver cancer.
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http://dx.doi.org/10.1016/j.aca.2021.338621DOI Listing
July 2021

A ratiometric fluorescent sensor for rapid detection of the pyroglutamate aminopeptidase-1 in mouse tumors.

J Mater Chem B 2021 Jun;9(22):4546-4554

Department of Radiology, Xinqiao Hospital, Army Medical University, Chongqing, 400037, P. R. China.

Pyroglutamate aminopeptidase-1 (PGP-1) is an important enzyme that plays an indispensable role in the process of inflammation. Up to now, few reports have been reported on the detection of PGP-1 activity in vivo and in vitro, and there are no reports on ratiometric detection. Here, the first red-emitting ratiometric fluorescent sensor (DP-1) for the specific detection of PGP-1 both in vivo and in vitro was designed and synthesized by using DCD-NH2 as the luminescent parent and pyroglutamate as a recognition group. After interacting with PGP-1, the amide bond is hydrolyzed by the enzyme and the color of the solution changes from yellow (λabs = 420 nm) to red (λabs = 520 nm), accompanied by obvious fluorescence emission wavelength change (from ∼564 nm to ∼616 nm). The probe has high specificity and sensitivity towards PGP-1 in about 10 min, and the DL is as low as 0.25 ng mL-1. Interestingly, under the stimulation of Freund's incomplete adjuvant and lipopolysaccharide, the imaging of DP-1 in HepG2 and RAW264 cells shows that the expression of PGP-1 is associated with inflammation. What's more, for the first, the imaging of a mouse tumor model confirms that the enzyme is closely related to the occurrence of some inflammation and tumor diseases. These results indicate that DP-1 can be used as an effective tool for real-time monitoring of PGP-1 levels both in vivo and in vitro and the study of inflammatory tumor pathology.
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http://dx.doi.org/10.1039/d1tb00372kDOI Listing
June 2021

Delayed thrombocytopenia as a rare but serious adverse event secondary to immune checkpoint inhibitor: a case report.

Ann Palliat Med 2021 May 17;10(5):5881-5886. Epub 2021 May 17.

Hematology Department, Tongde Hospital, Hangzhou, China.

Immune checkpoint inhibitors (ICIs) are a recent breakthrough in antitumor drugs, although their overall safety has not been fully defined. Compared to conventional chemotherapy, ICIs exhibit different patterns of immunotoxicity, and immune-related adverse events (irAEs) have an immunological basis that is more toxic than usual and have a broad spectrum of manifestations involving different organ systems. Early recognition of symptoms and timely intervention are very important in managing immune-related adverse events (irAEs). In this study, we report a case of delayed immune thrombocytopenia in a patient treated with nivolumab for small cell lung cancer (SCLC). We found that thrombocytopenia was associated with the presence of platelet antibodies, autoantibodies, and thyroglobulin antibodies, accompanied by a decrease in the number of helper T cells and regulatory T cells. Platelets returned to normal after the removal of antibodies by plasma exchanges and methylprednisolone. We hypothesized that thrombocytopenia in patients was an antibody-driven and T-cell-mediated process. Although these observations indirectly suggest that cytokine changes contribute to immune dysregulation during irAE, prospective validation is needed to explain the confounding etiologies that may contribute to cytokine dysregulation. Therefore, studying the relationship between T cell subpopulations, cytokines and irAE in a larger population may be crucial for identifying biomarkers for ICI.
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http://dx.doi.org/10.21037/apm-21-794DOI Listing
May 2021

Toxicological and bioactivity evaluation of blackcurrant press cake, sea buckthorn leaves and bark from Scots pine and Norway spruce extracts under a green integrated approach.

Food Chem Toxicol 2021 Jul 24;153:112284. Epub 2021 May 24.

Food Processing and Quality, Natural Resources Institute Finland (Luke), FI-02150, Espoo, Finland. Electronic address:

Aqueous extracts from blackcurrant press cake (BC), Norway spruce bark (NS), Scots pine bark (SP), and sea buckthorn leaves (SB) were obtained using maceration and pressurized hot water and tested for their bioactivities. Maceration provided the extraction of higher dry matter contents, including total phenolics (TPC), anthocyanins, and condensed tannins, which also impacted higher antioxidant activity. NS and SB extracts presented the highest mean values of TPC and antioxidant activity. Individually, NS extract presented high contents of proanthocyanidins, resveratrol, and some phenolic acids. In contrast, SB contained a high concentration of ellagitannins, ellagic acid, and quercetin, explaining the antioxidant activity and antibacterial effects. SP and BC extracts had the lowest TPC and antioxidant activity. However, BC had strong antiviral efficacy, whereas SP can be considered a potential ingredient to inhibit α-amylase. Except for BC, the other extracts decreased reactive oxygen species (ROS) generation in HCT8 and A549 cells. Extracts did not inhibit the production of TNF-alpha in lipopolysaccharide-stimulated THP-1 macrophages but inhibited the ROS generation during the THP-1 cell respiratory burst. The recovery of antioxidant compounds from these by-products is incentivized for high value-added applications.
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http://dx.doi.org/10.1016/j.fct.2021.112284DOI Listing
July 2021

Mid-term results of hybrid arch repair for DeBakey type I aortic dissection.

Chin Med J (Engl) 2021 May 19. Epub 2021 May 19.

Department of Cardiac Surgery, Beijing Anzhen Hospital, Beijing Institute of Heart, Lung and Blood Vessel Disease, Capital Medical University, Beijing 100029, China The State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.

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http://dx.doi.org/10.1097/CM9.0000000000001556DOI Listing
May 2021

CD40L/CD40 regulates adipokines and cytokines by H3K4me3 modification in epicardial adipocytes.

J Cardiovasc Pharmacol 2021 May 20. Epub 2021 May 20.

Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi, P.R. China.

Abstract: Epicardial adipose tissue (EAT) dysfunction mediates chronic inflammation by regulating inflammation-related adipokines and cytokines, and further promotes coronary artery disease (CAD) development. CD40L/CD40 is involved in multiple inflammatory pathways that contribute to various pathophysiological processes. However, the function of CD40L/CD40 in adipokine and cytokine expression and production in epicardial adipocytes remains unclear. The purpose of the present study was to explore the role and underlying mechanisms of CD40L/CD40 in adipokine and cytokine expression and production. We isolated adipocytes from EAT tissues of CAD and non-CAD patients. We noticed that CD40 was dramatically increased in EAT tissues of CAD patients. Loss-of-function and gain-of-function studies were performed. The results showed that CD40 silencing reduced recombinant CD40 ligand (rCD40L)-induced up-regulation of plasminogen activator inhibitor-1 (PAI-1), leptin, interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) mRNA levels and secretion. Overexpression of CD40 displayed the opposite results. In addition, rCD40L triggered mixed lineage leukemia protein-1 (MLL1) expression both in mRNA and protein levels. CD40 depletion apparently blocked MLL1 expression, whereas gain of function of CD40 resulted in augmentation of MLL1 levels. Interestingly, ChIP-qCPR analysis revealed that CD40 elimination dampened histone H3 lysine 4 trimethylation (H3K4me3) enrichment at PAI-1, leptin, IL-6 and MCP-1 promoter regions in the presence of rCD40L. The reverse pattern was observed upon ectopic expression of CD40. Most important, MLL1 silencing effectively reversed the promotive effects of CD40 on adipokine and cytokine secretion. Taken together, our findings suggest that CD40L/CD40 regulates adipokine and cytokine expression by H3K4me3 modification in adipocytes.
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http://dx.doi.org/10.1097/FJC.0000000000001060DOI Listing
May 2021

Functional connectivity evidence for state-independent executive function deficits in patients with major depressive disorder.

J Affect Disord 2021 Aug 21;291:76-82. Epub 2021 May 21.

Department of Psychiatry, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China; Mental Health Institute of Central South University, China National Clinical Research Center on Mental Disorders (Xiangya), China National Technology Institute on Mental Disorders, Hunan Technology Institute of Psychiatry, Hunan Key Laboratory of Psychiatry and Mental Health, Changsha, Hunan 410011, China. Electronic address:

Background: Persistent neurocognitive deficits are often associated with poor outcomes of major depressive disorder (MDD). Executive dysfunction is the most common cognitive deficit in MDD. However, it remains unclear which subcomponent of executive dysfunction is state-independent with distinct neural substrates.

Methods: A comprehensive neurocognitive test battery was used to assess four subcomponents of executive function (working memory, inhibition, shifting, and verbal fluency) in 95 MDD patients and 111 matched healthy controls (HCs). After 6 months of paroxetine treatment, 56 patients achieved clinical remission (rMDD) and completed the second-time neurocognitive test. Network-based statistics analysis was utilized to explore the changes in functional connectivity (FC).

Results: Compared with the HCs, all the four subcomponents of MDD patients were significantly impaired. After treatment, there was a significant improvement in working memory, inhibition, and verbal fluency in the rMDD group. And shifting and verbal fluency of the rMDD group remained impaired compared with the HCs. Fifteen functional connections were interrupted in the MDD group, and 11 connections remained in a disrupted state after treatment. Importantly, verbal fluency was negatively correlated with the disrupted FC between the right dorsal prefrontal cortex and the left inferior parietal lobule in patients with MDD and remitted MDD.

Limitations: The correlation analysis of the association between cognitive impairment and connectivity alterations precluded us from making causal inferences.

Conclusions: Verbal fluency is the potential state-independent cognitive deficit with distinct neural basis in patients with MDD.
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http://dx.doi.org/10.1016/j.jad.2021.04.080DOI Listing
August 2021

Oyster shells improve anaerobic dark fermentation performances of food waste: Hydrogen production, acidification performances, and microbial community characteristics.

Bioresour Technol 2021 Sep 12;335:125268. Epub 2021 May 12.

Department of Environmental Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.

Anaerobic dark fermentation (DF) performances of food waste (FW) were investigated using oyster shells. The different amount oyster shells(6%-12%(w/w)) were added to the DF system of FW. The result showed that the H production rate and cumulative H production improved after addition oyster shells. The highest H production rate and cumulative H production of 8% oyster shells addition group were 8.4 mL/(gVS·h) and 88.2 mL/gVS, which were 11.7%-30.6% and 17.4%-52.9% higher than those of the other test groups. TVFAs production, especially acetic and butyric acids improved after addition oyster shells. The highest TVFAs production was 19291.4 mg/L for 8% oyster shells added group, which was 90.24% higher than that of the unadded group. For 8% oyster shells added group, Lactobacillales, Gallicola, and Bacteroides were the dominant species at genus levels. Thus, the addition of an appropriate amount oyster shells could improve H production rate, cumulative H production, promote buffering capacity, enhance TVFAs production.
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http://dx.doi.org/10.1016/j.biortech.2021.125268DOI Listing
September 2021

The coupling of BOLD signal variability and degree centrality underlies cognitive functions and psychiatric diseases.

Neuroimage 2021 May 19;237:118187. Epub 2021 May 19.

State Key Laboratory of Cognitive Neuroscience and Learning & IDG/McGovern Institute of Brain Research, Beijing Normal University, Beijing 100875, PR China. Electronic address:

Brain signal variability has been consistently linked to functional integration; however, whether this coupling is associated with cognitive functions and/or psychiatric diseases has not been clarified. Using multiple multimodality datasets, including resting-state functional magnetic resonance imaging (rsfMRI) data from the Human Connectome Project (HCP: N = 927) and a Beijing sample (N = 416) and cerebral blood flow (CBF) and rsfMRI data from a Hangzhou sample (N = 29), we found that, compared with the existing variability measure (i.e., SD), the mean-scaled (standardized) fractional standard deviation of the BOLD signal (mfSD) maintained very high test-retest reliability, showed greater cross-site reliability and was less affected by head motion. We also found strong reproducible couplings between the mfSD and functional integration measured by the degree centrality (DC), both cross-voxel and cross-subject, which were robust to scanning and preprocessing parameters. Moreover, both mfSD and DC were correlated with CBF, suggesting a common physiological basis for both measures. Critically, the degree of coupling between mfSD and long-range DC was positively correlated with individuals' cognitive total composite scores. Brain regions with greater mismatches between mfSD and long-range DC were more vulnerable to brain diseases. Our results suggest that BOLD signal variability could serve as a meaningful index of local function that underlies functional integration in the human brain and that a strong coupling between BOLD signal variability and functional integration may serve as a hallmark of balanced brain networks that are associated with optimal brain functions.
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http://dx.doi.org/10.1016/j.neuroimage.2021.118187DOI Listing
May 2021

Neuroprotective Effect of Low-Intensity Pulsed Ultrasound on the Mouse MPTP/MPP Model of Dopaminergic Neuron Injury.

Ultrasound Med Biol 2021 May 16. Epub 2021 May 16.

Pediatric Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Engineering Research Center of Stem Cell Therapy, Children's Hospital of Chongqing Medical University, Chongqing, China. Electronic address:

Ultrasound mediated neuromodulation has been demonstrated to a safe treatment strategy in the field of neuroscience. In this study, low-intensity pulsed ultrasound (LIPUS) was used to treat Parkinson's disease (PD) models induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 1-methyl-4-phenylpyridinium (MPP) to explore the possibility of ultrasound neuroprotective effect on PD. The results demonstrated that LIPUS treatment can attenuate the central neurotoxicity of MPTP in mice, reduce the loss of tyrosine hydroxylase positive neurons in the substantia nigra pars compacta and decrease the apoptosis in the section of substantia nigra. The movement and balance dysfunctions in PD mice were improved with LIPUS treatment. In addition, we demonstrated that LIPUS can inhibit the decreased activity and increased apoptosis of dopaminergic neurons induced by MPP, restrain the accumulation of reactive oxygen species (ROS) and decrease of mitochondrial membrane potential caused by MPP. Moreover, LIPUS stimulation alone did not cause any cytotoxicity and tissue damage in our study. Taken together, the protective and regulatory effects of LIPUS on dopaminergic neurons make it possible as a new, safe and noninvasive treatment for PD.
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http://dx.doi.org/10.1016/j.ultrasmedbio.2021.03.034DOI Listing
May 2021

Stress memory gene FaHSP17.8-CII controls thermotolerance via remodeling PSII and ROS signaling in tall fescue.

Plant Physiol 2021 May 1. Epub 2021 May 1.

CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430074, China.

High-temperature is the most limiting factor in the growth of cool-season turfgrass. To cope with high-temperature stress, grass often adopt a memory response by remembering one past recurring stress and preparing a quicker and more robust reaction to the next stress exposure. However, little is known about how stress memory genes regulate the thermomemory response in cool-season turfgrass. Here, we characterized a transcriptional memory gene, Fa-heat shock protein 17.8 Class II (FaHSP17.8-CII) in a cool-season turfgrass species, tall fescue (Festuca arundinacea Schreb.). The thermomemory of FaHSP17.8-CII continued for more than four days and was associated with a high H3K4me3 level in tall fescue under heat stress (HS). Furthermore, heat acclimation or priming (ACC)-induced reactive oxygen species (ROS) accumulation and photosystem II (PSII) electron transport were memorable, and this memory response was controlled by FaHSP17.8-CII. In the fahsp17.8-CII mutant generated using CRISPR/Cas9, ACC+HS did not substantially block the ROS accumulation, the degeneration of chloroplast ultra-structure and the inhibition of PSII activity compared to HS alone. However, overexpression of FaHSP17.8-CII in tall fescue reduced ROS accumulation and chloroplast ultra-structure damage, and improved chlorophyll content and PSII activity under ACC+HS compared with that HS alone. These findings unveil a FaHSP17.8-CII-PSII-ROS module regulating transcriptional memory to enhance thermotolerance in cool-season turfgrass.
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http://dx.doi.org/10.1093/plphys/kiab205DOI Listing
May 2021

Dihydroartemisinin inhibits endothelial cell migration via the TGF-β1/ALK5/SMAD2 signaling pathway.

Exp Ther Med 2021 Jul 3;22(1):709. Epub 2021 May 3.

Laboratory of Microvascular Medicine, Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China.

Anti-angiogenesis therapy is a novel treatment method for malignant tumors. Endothelial cell (EC) migration is an important part of angiogenesis. Dihydroartemisinin (DHA) exhibits strong anti-angiogenic and anti-EC migration effects; however, the underlying molecular mechanisms are yet to be elucidated. The TGF-β1/activin receptor-like kinase 5 (ALK5)/SMAD2 signaling pathway serves an important role in the regulation of migration. The present study aimed to explore the effects of DHA treatment on EC migration and the TGF-β1/ALK5/SMAD2 signaling pathway. The effects of DHA on human umbilical vein EC migration were assessed using wound healing and Transwell assays. The effects of DHA on the TGF-β1/ALK5/SMAD2 signaling pathway were detected using western blotting. DHA exhibited an inhibitory effect on EC migration in the wound healing and Transwell assays. DHA treatment upregulated the expression levels of ALK5 and increased the phosphorylation of SMAD2 in ECs. SB431542 rescued the inhibitory effect of DHA during EC migration. DHA inhibited EC migration via the TGF-β1/ALK5/SMAD2-dependent signaling pathway, and DHA may be a novel drug for the treatment of patients with malignant tumors.
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http://dx.doi.org/10.3892/etm.2021.10141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120513PMC
July 2021

[Research progress of endogenous repair strategy in intervertebral disc].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 May;35(5):636-641

Department of Orthopedics, Northern Jiangsu People's Hospital, Yangzhou Jiangsu, 225000, P.R.China.

Objective: To review the research progress of endogenous repair strategy (ERS) in intervertebral disc (IVD).

Methods: The domestic and foreign literature related to ERS in IVD in recent years was reviewed, and its characteristics, status, and prospect in the future were summarized.

Results: The key of ERS in IVD is to improve the vitality of stem/progenitor cells in IVD or promote its migration from stem cell Niche to the tissue that need to repair. These stem/progenitor cells in IVD are derived from nucleus pulposus, annulus fibrosus, and cartilaginous endplate, showing similar biological characteristics to mesenchymal stem cells including the expression of the specific stem/progenitor cell surface markers and gene, and also the capacity of multiple differentiations potential. However, the development, senescence, and degeneration of IVD have consumed these stem/progenitor cells, and the harsh internal microenvironment further impair their biological characteristics, which leads to the failure of endogenous repair in IVD. At present, relevant research mainly focuses on improving the biological characteristics of endogenous stem/progenitor cells, directly supplementing endogenous stem/progenitor cells, biomaterials and small molecule compounds to stimulate the endogenous repair in IVD, so as to improve the effect of endogenous repair.

Conclusion: At present, ERS has gotten some achievements in the treatment of IVD degeneration, but its related studies are still in the pre-clinical stage. So further studies regarding ERS should be carried out in the future, especially experiments and clinical transformation.
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http://dx.doi.org/10.7507/1002-1892.202012070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175207PMC
May 2021

A new CcpA binding site plays a bidirectional role in carbon catabolism in .

iScience 2021 May 7;24(5):102400. Epub 2021 Apr 7.

Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China.

is widely used to produce various valuable products, such as food enzymes, industrial chemicals, and biocides. The carbon catabolite regulation process in the utilization of raw materials is crucial to maximizing the efficiency of this microbial cell factory. The current understanding of the molecular mechanism of this regulation is based on limited motif patterns in protein-DNA recognition, where the typical catabolite-responsive element (CRE) motif is "TGWNANCGNTNWCA". Here, CRE is identified and characterized as a new CRE. It consists of two palindrome arms of 6 nucleotides (AGCTTT/AAAGCT) and an intermediate spacer. CRE is involved in bidirectional regulation in a glucose stress environment. When AGCTTT appears in the 5' end, the regulatory element exhibits a carbon catabolite activation effect, while AAAGCT in the 5' end corresponds to carbon catabolite repression. Further investigation indicated a wide occurrence of CRE in the genome of .
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http://dx.doi.org/10.1016/j.isci.2021.102400DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091064PMC
May 2021

pH-Driven Reversible Assembly and Disassembly of Colloidal Gold Nanoparticles.

Front Chem 2021 29;9:675491. Epub 2021 Apr 29.

Department of Radiology, Xinqiao Hospital, Army Medical University, Chongqing, China.

Owing to the localized surface plasmon resonance (LSPR), dynamic manipulation of optical properties through the structure evolution of plasmonic nanoparticles has been intensively studied for practical applications. This paper describes a novel method for direct reversible self-assembly and dis-assembly of Au nanoparticles (AuNPs) in water driven by pH stimuli. Using 3-aminopropyltriethoxysilane (APTES) as the capping ligand and pH-responsive agent, the APTES hydrolyzes rapidly in response to acid and then condenses into silicon. On the contrary, the condensed silicon can be broken down into silicate by base, which subsequently deprotonates the APTES on AuNPs. By controlling condensation and decomposition of APTES, the plasmonic coupling among adjacent AuNPs could be reversible tuned to display the plasmonic color switching. This study provides a facile and distinctive strategy to regulate the reversible self-assembly of AuNPs, and it also offers a new avenue for other plasmonic nanoparticles to adjust plasmonic properties reversible self-assembly.
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http://dx.doi.org/10.3389/fchem.2021.675491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116534PMC
April 2021