Publications by authors named "Liang Zhang"

3,223 Publications

  • Page 1 of 1

Diagnostic accuracy of CCTA-derived versus angiography-derived quantitative flow ratio (CAREER) study: a prospective study protocol.

BMJ Open 2022 Jun 23;12(6):e055481. Epub 2022 Jun 23.

Department of Cardiology, Huadong Hospital Affiliated to Fudan University, Shanghai, China

Introduction: Coronary CT angiography (CCTA)-derived quantitative flow ratio (CT-QFR) is a novel non-invasive technology to assess the physiological significance of coronary stenoses, which enables fast and on-site computation of fractional flow reserve (FFR) from CCTA images. The objective of this investigator-initiated, prospective, single-centre clinical trial is to evaluate the diagnostic performance of CT-QFR with respect to angiography-derived QFR, using FFR as the reference standard.

Methods And Analysis: A total of 216 patients who have at least one lesion with a diameter stenosis of 30%-90% in an artery with ≥2.0 mm reference diameter will be enrolled in the study. FFR will be measured during invasive coronary angiography. CT-QFR and QFR will be assessed in two independent core laboratories in a blinded fashion. The primary endpoint is the diagnostic accuracy of CT-QFR in identifying haemodynamically significant coronary stenosis with FFR as the reference standard. The major secondary endpoint is the non-inferiority of CT-QFR compared with QFR in the patients without extensively calcified lesions.

Ethics And Dissemination: The study was approved by the Ethics Committee of Huadong Hospital Affiliated to Fudan University (2020K192). Outcomes will be disseminated through publications in peer-reviewed journals and presentations at scientific conferences.

Trial Registration Number: NCT04665817.
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http://dx.doi.org/10.1136/bmjopen-2021-055481DOI Listing
June 2022

Exploration of Potential Targets and Mechanisms of Fisetin in the Treatment of Non-Small-Cell Lung Carcinoma via Network Pharmacology and In Vitro Validation.

Evid Based Complement Alternat Med 2022 13;2022:2383527. Epub 2022 Jun 13.

Department of Oncology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China.

Purpose: The morbidity and fatality rates of non-small-cell lung cancer (NSCLC) were high, although a combination of multiple treatments was used. Fisetin, a small flavonoid compound, had shown anticancer activities. Thus, we aimed at exploring the mechanisms of Fisetin in the treatment of NSCLC.

Methods: TCMSP and Swiss target tools were used to screen the targets of Fisetin, and GeneCards was used to collect the genes related to NSCLC. The genes common to Fisetin and NSCLC were obtained by Venn analysis, whose possible functions were further annotated. A "Compound-Target-Disease" network was then constructed and hub genes were filtered. Also, molecular docking was performed to predict the binding abilities between Fisetin and the hub genes. Then, the effects of Fisetin on the expression of hub genes in lung adenocarcinoma cells were preliminarily evaluated in vitro.

Results: A total of 131 genes common to Fisetin and NSCLC were filtered out, which might be enriched in several biological processes including antioxidation, cell proliferation, and various signaling pathways, such as PI3K-Akt and IL-17 signaling pathways. Among them, PIK3R1, CTNNB1, JUN, EGFR, and APP might be the hub genes. Molecular docking indicated the close bond between Fisetin and them. Experiments implied a possible effect of Fisetin on the expression of hub genes in A549 cells.

Conclusion: The present study found a series of novel targets and pathways for Fisetin treating NSCLC. Multiple angles, targets, and pathways were involved in the biological processes, which need to be verified in further experiments.
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http://dx.doi.org/10.1155/2022/2383527DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208940PMC
June 2022

Positive Relationship Between Paroxysmal Vertigo and Right-to-Left Shunt: A Large Observational Study.

Front Neurol 2022 3;13:927853. Epub 2022 Jun 3.

Department of Neurology, Tongde Hospital of Zhejiang Province, Hangzhou, China.

Background: The association between paroxysmal vertigo and right-to-left shunt (RLS) is rarely reported. This study investigates the prevalence and correlation of RLS in patients with different paroxysmal vertigo diseases.

Methods: Patients with paroxysmal vertigo from seven hospitals in China were included in this observational study between 2017 and 2021. Migraine patients within the same period were included for comparison. Demographic data and medical history were collected; contrast transthoracic echocardiography was performed; and the clinical features, Dizziness Handicap Inventory, and incidence of RLS in each group were recorded.

Results: A total of 2,751 patients were enrolled. This study's results demonstrated that the proportion of RLS in patients with benign recurrent vertigo (BRV) and vestibular migraine (VM) was significantly higher than that in patients with benign paroxysmal positional vertigo, Meniere's disease, and vestibular paroxysmia ( < 0.05). No statistical difference was shown between the frequency of RLS in patients with BRV and those with migraine and VM. A positive correlation was shown between the RLS grade and Dizziness Handicap Inventory scores of patients with VM and BRV ( < 0.01) after effectively controlleding the effect of confounding variables.

Conclusions: RLS was significantly associated with BRV and VM. RLS may be involved in the pathogeneses of BRV and VM and may serve as a differential reference index for the paroxysmal vertigo.

Trial Registration: CHRS, NCT04939922, registered 14 June 2021- retrospectively registered, https://register.clinicaltrials.gov.
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http://dx.doi.org/10.3389/fneur.2022.927853DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203692PMC
June 2022

The Mediating Role of Emotion Regulation Strategies in the Relationship Between Big Five Personality Traits and Anxiety and Depression Among Chinese Firefighters.

Front Public Health 2022 3;10:901686. Epub 2022 Jun 3.

College of Education for the Future, Beijing Normal University at Zhuhai, Zhuhai, China.

Identification of protective factors to prevent firefighters' anxiety and depression is meaningful. We explored whether emotion-regulation strategies mediate the relationship between personality traits and anxiety and depression among Chinese firefighters. Approximately, 716 Chinese firefighters were recruited and completed the Emotion Regulation Questionnaire (ERQ), Self-Rating Anxiety Scale (SAS), Self-Rating Depression Scale (SDS), and Big Five Inventory-2 (BFI-2) Scale. Results ( = 622) indicated that only negative emotionality traits could predict anxiety symptoms. Meanwhile, the multilevel mediation effect analyses showed that conscientiousness through cognitive reappraisal could reduce anxiety and depression symptoms in Chinese firefighters. Our findings clarify Chinese firefighters' underlying emotion-regulation process between personality traits and anxiety and depression. Implications, limitations, and future directions are discussed.
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http://dx.doi.org/10.3389/fpubh.2022.901686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205204PMC
June 2022

Single-Cell Transcriptomics Uncover Key Regulators of Skin Regeneration in Human Long-Term Mechanical Stretch-Mediated Expansion Therapy.

Front Cell Dev Biol 2022 30;10:865983. Epub 2022 May 30.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Tissue expansion is a commonly performed therapy to grow extra skin for reconstruction. While mechanical stretch-induced epidermal changes have been extensively studied in rodents and cell culture, little is known about the mechanobiology of the human epidermis . Here, we employed single-cell RNA sequencing to interrogate the changes in the human epidermis during long-term tissue expansion therapy in clinical settings. We also verified the main findings at the protein level by immunofluorescence analysis of independent clinical samples. Our data show that the expanding human skin epidermis maintained a cellular composition and lineage trajectory that are similar to its non-expanding neighbor, suggesting the cellular heterogeneity of long-term expanded samples differs from the early response to the expansion. Also, a decrease in proliferative cells due to the decayed regenerative competency was detected. On the other hand, profound transcriptional changes are detected for epidermal stem cells in the expanding skin versus their non-expanding peers. These include significantly enriched signatures of C-FOS, EMT, and mTOR pathways and upregulation of AREG and SERPINB2 genes. CellChat associated ligand-receptor pairs and signaling pathways were revealed. Together, our data present a single-cell atlas of human epidermal changes in long-term tissue expansion therapy, suggesting that transcriptional change in epidermal stem cells is the major mechanism underlying long-term human skin expansion therapy. We also identified novel therapeutic targets to promote human skin expansion efficiency in the future.
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http://dx.doi.org/10.3389/fcell.2022.865983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9195629PMC
May 2022

The Impact of Customer Experience and Customer Engagement on Behavioral Intentions: Does Competitive Choices Matters?

Front Psychol 2022 25;13:864841. Epub 2022 May 25.

Departamento de Ingeniería Industrial, Facultad de Ingeniería, Universidad Católica de la Santísima Concepción, Concepción, Chile.

The study aims to analyze behavioral intentions influenced by customer engagement, experience, and identification moderated by competitive choices in the granite sector of Pakistan. The study has been carried out through primary data analysis of cross-sectional approach in the transition to a sustainable economy. In total, 400 questionnaires were distributed, for which only 216 were filled and usable with a response rate of 54%-collected data from the production managers and units. In contrast, missed mine holders and labor analyzed the data in SPSS and AMOS to run various tests, i.e., reliability, correlation analysis, regression, moderation regression, and confirmatory factor analysis. The study findings indicate a positive and significant relationship and effect among the variables. The reviews might contain some biases. Therefore, this study recommended adopting a probability sampling technique for future studies. The study results in a positive manner indicating customer service involvement as a significant factor in behavioral intention despite competitive options.
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http://dx.doi.org/10.3389/fpsyg.2022.864841DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196106PMC
May 2022

Correction to: Homologous targeting nanoparticles for enhanced PDT against osteosarcoma HOS cells and the related molecular mechanisms.

J Nanobiotechnology 2022 Jun 16;20(1):286. Epub 2022 Jun 16.

Department of Orthopedic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.

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http://dx.doi.org/10.1186/s12951-022-01492-9DOI Listing
June 2022

Segregation of effect between granules and flocs in PN/A system treating acrylic fiber wastewater: Performance and mechanism.

Chemosphere 2022 Oct 13;304:135344. Epub 2022 Jun 13.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing, 100124, China.

Nitrogen removal of petrochemical wastewater through partial nitritation/anammox (PN/A) is appealing, but its feasibility and stability under toxic inhibition remain unclear. This study started a PN/A granular sludge system in a membrane bioreactor and fed it with diluted acrylic fiber wastewater. During long-term operation, the nitritation and anammox performance remained stable at a 30% volume ratio, and declined with increasing volume ratio, resulting in deteriorated nitrogen removal. Meanwhile, the short-term inhibition batch tests further showed that ammonia oxidation bacteria (AOB) in the flocs were suppressed while anammox bacteria (AnAOB) in the granules were not affected. Further analysis indicated suppression of AnAOB over the long-term operation, which was mainly caused by the disintegration of granules as demonstrated by sludge morphology. This selective inhibition is associated with variational sludge morphology, and the distribution of functional bacteria plays an important role in the feasibility and stability of PN/A treating acrylic fiber wastewater. As above, this study demonstrated the feasibility of PN/A for acrylic fiber wastewater treatment, but wastewater dilution or pre-treatment is still required for efficient nitrogen removal.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135344DOI Listing
October 2022

High expression of predict poor prognosis of colorectal cancer and influence the immune infiltrating level.

Transl Cancer Res 2022 May;11(5):981-992

Department of Hematology and Oncology, Hangzhou Normal University Affiliated Hospital, Hangzhou, China.

Background: Colorectal cancer (CRC) is the third most common malignancy. Immunoinvasion of tumor microenvironment was positively correlated with overall cancer survival. is a little-reported gene in tumors, and its mechanism of action remains unclear. Our study used The Cancer Genome Atlas (TCGA) data to evaluate the role of in CRC.

Methods: Software R3.6.3 analyzed the expression of in CRC. We assessed the impact of on the survival of CRC patients through a survival module. The CRC data set was then downloaded from TCGA. Logistic regression was used to analyze the correlation between clinical information and expression. Cox regression analysis showed that clinicopathological characteristics of TCGA patients were correlated with overall survival. In addition, we used ssGSEA to explore the correlation between and tumor immune invasion.

Results: High expression of is significantly correlated with advanced pathological stage and poor overall survival. In multivariate analysis, the up-regulated expression, high tumor stage, and distant metastasis are independent prognostic factors of poor prognosis. Moreover, a negative correlation between increased expression and immune infiltrating T cells and neutrophils cells was established.

Conclusions: High expression of was associated with poor prognosis of CRC and affected the proportion of immune cells such as T cells, neutrophils, and NK cells. These results suggest that can be used as a potential biomarker to evaluate the prognosis and level of immune invasion in CRC.
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http://dx.doi.org/10.21037/tcr-21-2674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189158PMC
May 2022

Initiation of aboveground organ primordia depends on combined action of auxin, ERECTA family genes, and PINOID.

Plant Physiol 2022 Jun 15. Epub 2022 Jun 15.

Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, TN, 37996, USA.

Leaves and flowers are produced by the shoot apical meristem (SAM) at a certain distance from its center, a process that requires the hormone auxin. The amount of auxin and the pattern of its distribution in the initiation zone determine the size and spatial arrangement of organ primordia. Auxin gradients in the SAM are formed by PIN-FORMED (PIN) auxin efflux carriers whose polar localization in the plasma membrane depends on the protein kinase PINOID (PID). Previous work determined that ERECTA (ER) family genes (ERfs) control initiation of leaves. ERfs are plasma membrane receptors that enable cell-to-cell communication by sensing extracellular small proteins from the EPIDERMAL PATTERNING FACTOR/EPF-LIKE (EPF/EPFL) family. Here, we investigated whether ERfs regulate initiation of organs by altering auxin distribution or signaling in Arabidopsis (Arabidopsis thaliana). Genetic and pharmacological data suggested that ERfs do not regulate organogenesis through PINs while transcriptomics data showed ERfs do not alter primary transcriptional responses to auxin. Our results indicated that in the absence of ERf signaling the peripheral zone cells inefficiently initiate leaves in response to auxin signals and that increased accumulation of auxin in the er erecta-like1 (erl1) erl2 SAM can partially rescue organ initiation defects. We propose that both auxin and ERfs are essential for leaf initiation and that they have common downstream targets. Genetic data also indicated that the role of PID in initiation of cotyledons and leaves cannot be attributed solely to regulation of PIN polarity, and PID is likely to have other functions in addition to regulation of auxin distribution.
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http://dx.doi.org/10.1093/plphys/kiac288DOI Listing
June 2022

Transcriptome Analysis Reveals the Alternative Splicing Changes in the Immune-Related Genes of the Giant Panda (), in Response to the Canine Distemper Vaccine.

Zoolog Sci 2022 Jun;39(3):275-285

Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China,

Canine distemper virus (CDV) is a highly fatal virus to the giant panda (). Although vaccination is a key preventative measure in captive giant pandas, the immune response of giant pandas after vaccination remains unclear. Therefore, this study focuses on differential alternative splicing (DAS) events of giant pandas before and after vaccination to investigate the role of alternative splicing in the immune response of giant pandas after CDV vaccination. In this study, we identified 1113 DAS genes, which had 1288 DAS events. The KEGG functional enrichment analysis of DAS genes showed enrichment of some DNA damage repair and immune-related pathways. In the combined analysis of DAS and differentially expressed genes (from our previous research), we identified 66 differentially expressed genes with a DAS event, and found that some important immune-related genes, such as IL15, IL18, IL18RAP, CHUK, IFI44, CD40, and CD46 underwent DAS events and were involved in the immune response of giant pandas after CDV vaccination. We describe here the alternative splicing events of giant pandas after CDV vaccination for the first time and show that the results indicated that alternative splicing has an important role in regulating the immune response of giant pandas after vaccination.
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http://dx.doi.org/10.2108/zs210078DOI Listing
June 2022

Implementation of various bowl designs in an HPDI natural gas engine focused on performance and pollutant emissions.

Chemosphere 2022 Sep 10;303(Pt 3):135275. Epub 2022 Jun 10.

School of Vehicle and Energy, Yanshan University, Qinhuangdao, 066004, China. Electronic address:

The air-fuel mixture preparation in pilot spray-ignited natural gas engines is primarily dominated by piston bowl profiles and fuel injection strategy. Piston bowl geometry is regarded as the crucial point in controlling engine pollutant emissions. In the present work, the SAGE combustion model was applied coupled with a general reaction kinetic mechanism. The engine model was validated with experimental data achieved from a Cummins ISX 400 engine, and good agreement between predicted and measured in-cylinder pressure and heat release rate was obtained. The influence of various piston bowl designs, including Mexican-hat geometry, double-lip geometry, bow geometry, and toroidal geometry, on the combustion process, engine performance, and pollutant emissions of a high-pressure direct-injection natural gas engine have been studied and analyzed numerically. The present study confirms the benefit of the piston bowl design as a beneficial tool to enhance the performance and pollutant emissions of the pilot diesel-ignited natural gas engine. Results showed that different chamber shapes slightly influence the combustion initiation, and the difference in in-cylinder pressure presents noticeable as the combustion continues. A higher turbulent kinetic energy improves the flow movement and facilitates the mixture formation in the cylinder. However, the combustion behavior is unwished caused by the improper injection angle of natural gas. Increasing the recess depth of combustion chambers reduces NO formations at the price of sacrificing fuel economy. For the bow combustion chamber design, the NO emission declined by 31.1%, while the indicated specific fuel consumption increased by 5.5% compared with the original engine. Although the indicated mean effective pressure and specific fuel consumption of the optimal double-lip geometry almost remain the same, NO emissions can be reduced by 16.7% compared with the base design.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135275DOI Listing
September 2022

Gastrointestinal metastatic signet ring cell breast cancer in young females: a case report.

Gland Surg 2022 May;11(5):943-952

Department of Breast and Thyroid Surgery, Shandong Provincial Maternal and Child Health Care Hospital, Jinan, China.

Background: Signet ring cell carcinoma (SRCC) is characterized by strong invasiveness and rapid progression. It occurs mostly in young and middle-aged patients, and early patients may have no clinical symptoms. Gastric SRCC with breast cancer metastasis is relatively rare. It often presents challenges for clinicians and pathologists and may lead to an absolutely different therapeutic strategy.

Case Description: In this paper, we report on a 37-year-old woman who was admitted to the hospital with a left breast mass discovered 5 days earlier, the mass was occasionally painful, and there was no skin swelling, skin depression, or other abnormalities. The initial diagnosis considered her to have a left breast tumor. The patient was previously healthy with no family history of tumor. Considering the possibility of malignant lesions, she underwent resection of the left breast tumor and surrounding tissue. Postoperative pathological findings suggested SRCC (left breast mass). Although the patient had no history of gastrointestinal tumors, considering that SRCC can also appear in the gastrointestinal tract and other organs. We performed gastroscopy on the patient, showed an ulcerative mass in the greater curvature of the gastric body, with irregular nodular uplift of the surrounding mucosa. The excised breast lesions were analyzed by immunohistochemistry, and the pathological result showed SRCC (left breast tumor). Combined with the results of immunohistochemistry, it was consistent with gastrointestinal metastasis. Through our multi-faceted differential diagnosis, the final diagnosis of the patient was clear, which not only bought time for the patient's subsequent treatment, but also avoided misdiagnosis and blind treatment due to the particularity and rarity of the case.

Conclusions: Gastric cancer should be considered when breast tumors show SRCC without lesion. Signet ring cell gastric cancer (occult) should be excluded even if the patient has no family history of gastric cancer. It is important to distinguish metastatic cancer from primary breast cancer to avoid misdiagnosis and blind treatment due to the particularity of the case, at which point an early recognition can be made and an optimal treatment plan can be chosen.
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http://dx.doi.org/10.21037/gs-22-242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177283PMC
May 2022

Hypercholanaemia from sodium-taurocholate cotransporting polypeptide deficiency in pregnancy.

Clin Res Hepatol Gastroenterol 2022 Jun 8:101973. Epub 2022 Jun 8.

Maternal Intensive Care Unit, Guangdong Women and Children Hospital, Guangzhou, China, 511400.

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http://dx.doi.org/10.1016/j.clinre.2022.101973DOI Listing
June 2022

The OsFTIP6-OsHB22-OsMYBR57 module regulates drought response in rice.

Mol Plant 2022 Jun 9. Epub 2022 Jun 9.

State Key Laboratory of Rice Biology, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China. Electronic address:

Plants have evolved a series of mechanisms to adapt to drought stress. Transcription factors are crucial for plant response to various environmental signals by modulating the expression of numerous stress-responsive genes. However, how the crosstalk between different transcription factor families orchestrates to initiate the key transcriptional network responding to stress, and the role of posttranscriptional modification of transcription factors, especially in the cellular localization/trafficking of stress response in rice, are still largely unknown. Here, we isolated an Osmybr57 mutant displaying a drought-sensitive phenotype in the genetic screening of drought stress. We have found that OsMYBR57, an MYB-related protein, directly regulates several key drought-related OsbZIPs to respond to drought treatment. Further studies reveal that OsMYBR57 interacts with a homeodomain transcription factor, OsHB22, which also plays a positive role in drought signaling. We further demonstrate that OsFTIP6 interacts with OsHB22 and promotes the nucleocytoplasmic translocation of OsHB22 into the nucleus, where OsHB22 cooperates with OsMYBR57 to regulate the expression of drought-related genes. Our findings have revealed a mechanistic framework underlying the OsFTIP6-OsHB22-OsMYBR57 module mediating drought response in rice. The OsFTIP6-mediated OsHB22 nucleocytoplasmic shuttling and OsMYBR57-OsHB22 regulatory modes of OsbZIPs transcription ensure precise control of expression of OsLEA3 and Rab21, thereby regulating the response to water deficiency in rice.
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http://dx.doi.org/10.1016/j.molp.2022.06.003DOI Listing
June 2022

Multistage Combination Classifier Augmented Model for Protein Secondary Structure Prediction.

Front Genet 2022 23;13:769828. Epub 2022 May 23.

College of Mechanical Engineering, Dalian University of Technology, Dalian, China.

In the field of bioinformatics, understanding protein secondary structure is very important for exploring diseases and finding new treatments. Considering that the physical experiment-based protein secondary structure prediction methods are time-consuming and expensive, some pattern recognition and machine learning methods are proposed. However, most of the methods achieve quite similar performance, which seems to reach a model capacity bottleneck. As both model design and learning process can affect the model learning capacity, we pay attention to the latter part. To this end, a framework called Multistage Combination Classifier Augmented Model (MCCM) is proposed to solve the protein secondary structure prediction task. Specifically, first, a feature extraction module is introduced to extract features with different levels of learning difficulties. Second, multistage combination classifiers are proposed to learn decision boundaries for easy and hard samples, respectively, with the latter penalizing the loss value of the hard samples and finally improving the prediction performance of hard samples. Third, based on the Dirichlet distribution and information entropy measurement, a sample difficulty discrimination module is designed to assign samples with different learning difficulty levels to the aforementioned classifiers. The experimental results on the publicly available benchmark CB513 dataset show that our method outperforms most state-of-the-art models.
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http://dx.doi.org/10.3389/fgene.2022.769828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170271PMC
May 2022

Detection of NDM-1-Positive from Bacteremia by Using Whole-Genome Sequencing.

Infect Drug Resist 2022 2;15:2835-2841. Epub 2022 Jun 2.

Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, People's Republic of China.

Purpose: Nosocomial infections caused by New Delhi metallo-β-lactamase (NDM)-producing bacteria are prevalent worldwide. However, such diseases caused by NDM-producing had never been reported. Our study aimed to elucidate the genomic characteristics of NDM-1-producing isolated from hospital patients.

Methods: Bacterial genomic features and possible origins were assessed by whole-genome sequencing (WGS) and phylogenetic analysis. Subsequent investigations include antimicrobial susceptibility testing and multilocus sequence typing (MLST).

Results: We identified here two NDM-1-producing isolates from bacteremia. Susceptibility testing showed that two isolates were multi-drug resistant and shared a similar resistance profile and were only sensitive to amikacin and trimethoprim/sulfamethoxazole. Both isolates carry the carbapenem resistance gene and also have antibiotic resistance genes such as β-lactams, AmpC enzymes, macrolides, aminoglycosides, and quinolones. S1-PFGE and Southern blot analysis were negative. Whole-genome sequencing and comparative analysis revealed that these two isolates shared a close relationship.

Conclusion: To the best of our knowledge, this work describes the first detection of non-plasmid encoded in . The isolated in this study has a broad drug resistance spectrum. Phenotypic and molecular analysis indicated the two isolates belong to the same clone. Routine genomic surveillance of this species is now necessary to effectively curb the further dissemination of carbapenem-resistant bacteria in the region.
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http://dx.doi.org/10.2147/IDR.S360353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169836PMC
June 2022

Magnetoresistance studies of two-dimensional FeGeTenano-flake.

J Phys Condens Matter 2022 Jun 24;34(34). Epub 2022 Jun 24.

College of Information Science and Electronic Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.

The magneto-transport properties of two-dimensional (2D) FeGeTe(FGT) nano-flakes are carefully investigated with the variation of the temperature and the direction of the applied magnetic field (). Four magnetoresistance (MR) behavior are obtained at different temperatures withparalleling the flake's surface, because of the competition between the merging of different domains, spin fluctuation, and the spin momentum flipping. Different from the reported negative MR of bulk FGT, 2D FGT shows a positive MR behavior with the increase ofat a low temperature in a lowrange, owning to the domination of the spin momentum flipping induced by the weakening of the coupling between different layers with the decrease of the thickness of the FGT flake. The angle-dependence of the FGT MR is also investigated and can be well explained by the competition mentioned above.
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http://dx.doi.org/10.1088/1361-648X/ac76feDOI Listing
June 2022

Stress-induced higher vein density in C3-C4 intermediate Moricandia suffruticosa under drought and heat stress.

J Exp Bot 2022 Jun 8. Epub 2022 Jun 8.

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan, Hubei, People's Republic of China.

C4 evolution involves multiple anatomical and physiological modifications, yet our knowledge of its genetic regulation remains elusive. In this study, systematic analyses were conducted between C3-C4 intermediate Moricandia suffruticosa and its C3 relative Brassica napus. We found that M. suffruticosa leaves had significantly higher vein density than those of B. napus, and the vein density was further increased in M. suffruticosa under drought and heat stress. Moreover, the bundle sheath distance decreased and the number of centripetal chloroplasts in bundle sheath cells was found to be altered in M. suffruticosa leaves under drought and heat treatments. These results suggest that abiotic stress can induce a change in an intermediate C3-C4 anatomy towards a C4-like anatomy in land plants. By integrating drought and heat factors, co-expression network and comparative transcriptome analyses between M. suffruticosa and B. napus effectively revealed that inducible auxin signaling regulated vascular development and autophagy-related vesicle trafficking process were associated with this stress-induced anatomy change. Overexpressing three candidate genes, MsERF02, MsSCL01 and MsDOF01, increased leaf vein density and/or also enhanced photosynthetic assimilation and drought adaptability in the transgenic lines. The findings of this study may improve our understanding of the genetic regulation and evolution of C4 anatomy.
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http://dx.doi.org/10.1093/jxb/erac253DOI Listing
June 2022

Dual honeycomb-superlattice enables double-high activity and reversibility of anion redox for sodium-ion battery layered cathodes.

Angew Chem Int Ed Engl 2022 Jun 8. Epub 2022 Jun 8.

Nanjing University, Xianlin 168, Nanjing, CHINA.

Anion redox contributes to the anomalous capacity exceeding theoretical limit of layered oxides. However, double-high activity and reversibility is challenging due to the structural rearrangement and potential oxygen loss. Here, we propose a strategy of constructing dual honeycomb-superlattice structure in Na 2/3 [Li 1/7 Mn 5/14 ][Mg 1/7 Mn 5/14 ]O 2 to simultaneously realize high activity and reversibility of lattice O redox. Theoretical simulation and electrochemical tests show that [Li 1/7 Mn 5/14 ] superlattice units remarkably trigger the anion redox activity and enable to deliver a record capacity of 285.9 mA g -1 in layered sodium-ion battery cathodes. Nuclear magnetic resonance and in-situ X-ray diffraction reveal that [Mg 1/7 Mn 5/14 ] superlattice units are beneficial to the structure and anion redox reversibility , where Li + reversibly shuttles between Na layers and transition-metal slabs in contrast to the absence of [Mg 1/7 Mn 5/14 ] units. Our findings underline the importance of multifunctional units and provide a path to advanced battery materials.
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http://dx.doi.org/10.1002/anie.202206625DOI Listing
June 2022

Claudin-5a is essential for the functional formation of both zebrafish blood-brain barrier and blood-cerebrospinal fluid barrier.

Fluids Barriers CNS 2022 Jun 3;19(1):40. Epub 2022 Jun 3.

Affiliated Hospital of Guangdong Medical University & Key Laboratory of Zebrafish Model for Development and Disease of Guangdong Medical University, Zhanjiang, 524001, China.

Background: Mammalian Claudin-5 is the main endothelial tight junction component maintaining blood-brain barrier (BBB) permeability, while Claudin-1 and -3 seal the paracellular space of choroid plexus (CP) epithelial cells contributing to the blood-cerebrospinal fluid barrier (BCSFB). In zebrafish, two paralogs of claudin-5a and -5b are expressed while their roles in the formation of BBB and BCSFB are unclear.

Methods: The expression patterns of Claudin-5a and -5b in zebrafish brains were systematically analyzed by immunofluorescence (IF) assay. The developmental functions of Claudin-5a and -5b were characterized by generating of claudin-5a and -5b mutants respectively. Meanwhile, the cerebral inflammation and cell apoptosis in claudin-5a were assessed by live imaging of transgenic zebrafish, RT-qPCR, IF, and TUNEL assay. The integrity of BBB and BCSFB was evaluated by in vivo angiographic and dye permeation assay. Finally, RT-qPCR, whole-mount RNA in situ hybridization (WISH), and transmission electron microscopy (TEM) analyses were performed to investigate the development of cerebral vessels and choroid plexus.

Results: We showed that Claudin-5a and -5b are both expressed in zebrafish cerebrovascular endothelial cells (ECs). In addition, Claudin-5a was strongly expressed in CP epithelial cells. Loss of Claudin-5b showed no effect on zebrafish vasculogenesis or BBB function. In contrast, the knockout of claudin-5a caused a lethal phenotype of severe whole-brain oedema, ventricular dilatation, and cerebral hernia in zebrafish larvae, although the cerebral vasculogenesis and the development of CP were not altered. In claudin-5a , although ultrastructural analysis of CP and cerebral capillary showed intact integrity of epithelial and endothelial tight junctions, permeability assay indicated a disruption of both BBB and BCSFB functions. On the molecular level, it was found that ZO-1 was upregulated in the CP epithelium of claudin-5a, while the notch and shh pathway responsible for CP development was not affected due to loss of Claudin-5a.

Conclusions: Our findings verified a non-functional role of zebrafish Claudin-5b in the BBB and identified Claudin-5a as the ortholog of mammalian Claudin-5, contributing to the development and the functional maintenance of both BBB and BCSFB.
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http://dx.doi.org/10.1186/s12987-022-00337-9DOI Listing
June 2022

A fungal dioxygenase CcTet serves as a eukaryotic 6mA demethylase on duplex DNA.

Nat Chem Biol 2022 Jun 2. Epub 2022 Jun 2.

Department of Pharmacology and Chemical Biology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

N-methyladenosine (6mA) is a DNA modification that has recently been found to play regulatory roles during mammalian early embryo development and mitochondrial transcription. We found that a dioxygenase CcTet from the fungus Coprinopsis cinerea is also a dsDNA 6mA demethylase. It oxidizes 6mA to the intermediate N-hydroxymethyladenosine (6hmA) with robust activity of 6mA-containing duplex DNA (dsDNA) as well as isolated genomics DNA. Structural characterization revealed that CcTet utilizes three flexible loop regions and two key residues-D337 and G331-in the active pocket to preferentially recognize substrates on dsDNA. A CcTet D337F mutant protein retained the catalytic activity on 6mA but lost activity on 5-methylcytosine. Our findings uncovered a 6mA demethylase that works on dsDNA, suggesting potential 6mA demethylation in fungi and elucidating 6mA recognition and the catalytic mechanism of CcTet. The CcTet D337F mutant protein also provides a chemical biology tool for future functional manipulation of DNA 6mA in vivo.
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http://dx.doi.org/10.1038/s41589-022-01041-3DOI Listing
June 2022

Effects of particle size distribution of potato starch granules on rheological properties of model dough underwent multiple freezing-thawing cycles.

Food Res Int 2022 Jun 9;156:111112. Epub 2022 Mar 9.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, PR China. Electronic address:

In order to elucidate the mechanism underlying the effect of different-sizes potato starch on dough during freeze-thaw treatment, the rheological properties, moisture distribution, secondary structure, and relative crystallinity of dough were investigated. The results showed that the storage modulus (G'), loss modulus (G″), and complex modulus (|G*|) of dough increased as a result of the freeze-thaw treatment, and the effect was more obvious as the starch granular sizes increased. The higher β -sheet and T contents reflected the higher viscoelasticity and freeze-thaw sensitivity, which may be related to the higher degree expansion of amylose. In contrast, dough with small-sized starch had higher intermolecular interactions, and denser structure, lower water migration, showing that this has better resistance and higher stability. The presented mechanisms may contribute to the better understanding of the effects of freeze-thaw process on model dough properties.
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http://dx.doi.org/10.1016/j.foodres.2022.111112DOI Listing
June 2022

Microbiome-metabolomics insights into the feces of high-fat diet mice to reveal the anti-obesity effects of yak (Bos grunniens) bone collagen hydrolysates.

Food Res Int 2022 Jun 17;156:111024. Epub 2022 Feb 17.

National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China. Electronic address:

In this study, a twelve-week intervention was conducted to investigate the anti-obesity effects of yak bone collagen hydrolysates (YBCH) on mice with a high-fat diet. The obesity-associated phenotypes of mice were detected and the feces of mice were jointly analyzed by 16S rRNA gene sequencing and untargeted metabolomics. Results indicated that supplementation with YBCH could ameliorate the obesity-associated phenotypes of mice, especially with the medium dose (MD) and high dose (HD) of YBCH. Compared with the high-fat diet control (HC) group, the ratio of Firmicutes and Bacteroidetes in the fecal microbiota of the low dose (LD), MD, and HD groups was separately decreased by 29.83 %, 70.85 %, and 75.70 %. Lachnospiraceae and Muribaculaceae were the key bacteria for the YBCH intervention, which might be attributed to their different substrate preferences. The joint analysis of the 16S rRNA gene sequencing and untargeted metabolomics suggested that the anti-obesity effects of YBCH might be achieved by up-regulating the arginine and proline metabolism and down-regulating the aromatic amino acids metabolism via the gut microbiota.
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http://dx.doi.org/10.1016/j.foodres.2022.111024DOI Listing
June 2022

Diversity and dynamics of microbial communities during spontaneous fermentation of Cabernet Sauvignon (Vitis vinifera L.) from different regions of China and their relationship with the volatile components in the wine.

Food Res Int 2022 Jun 14;156:111372. Epub 2022 May 14.

College of Enology, Northwest A&F University, No. 22, Xinong Road, Yangling, Shaanxi 710000, PR China; Shaanxi Engineering Research Center for Viti-Viniculture, No. 22, Xinong Road, Yangling, Shaanxi 710000, PR China; China Wine Industry Technology Institute, Zhongguancun Innovation Center, Yinchuan, Ningxia 750000, PR China. Electronic address:

Wine presents the most distinct geographic signatures among all agricultural products, and these geographic characteristics of the wine are enhanced by the actions of indigenous microorganisms. China is one of the largest wine-producing and consuming nations in the world. The wine-related microbial resources are abundant in China, although their geographic distribution patterns and their contribution to the aroma of wine remain to be elucidated. In the present study, Cabernet Sauvignon samples from four wine-producing regions in China were subjected to high-throughput sequencing and HS-SPME-GC-MS techniques to study the diversity and dynamics of the microorganisms that were present during the spontaneous fermentation process and to provide a preliminary understanding of the contribution of these microorganisms to the volatile components of the wine. The results revealed significant differences in the microbial diversity in the grape musts among different vineyards, which led to significant differences in the composition of the volatile metabolites of the produced wine. Moreover, while the fermentation process was observed to have reshaped the structure of the microbial community, specific characteristics of the vineyard were retained at the completion of the fermentation process. The associations between microbiota diversity and wine chemicals suggested that the dominant species during the fermentation process largely determined the volatile components of the wine. The present study enhances the understanding of Chinese wine terroir and provides a scientific basis for maintaining the regional microbial biodiversity to sustain viticulture and winemaking.
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http://dx.doi.org/10.1016/j.foodres.2022.111372DOI Listing
June 2022

Author Correction: The Oligodendrocyte Transcription Factor 2 OLIG2 regulates transcriptional repression during myelinogenesis in rodents.

Nat Commun 2022 Jun 1;13(1):3164. Epub 2022 Jun 1.

Department of Neurosurgery and Department of Neuroscience, the First Affiliated Hospital of Xiamen University, State Key Laboratory of Cellular Stress Biology, School of Medicine, Xiamen University, Fujian, China.

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http://dx.doi.org/10.1038/s41467-022-30945-wDOI Listing
June 2022

A Multiple-Target Simultaneous Detection Method for Immunosorbent Assay and Immunospot Assay.

Anal Chem 2022 Jun 1;94(24):8704-8714. Epub 2022 Jun 1.

Kunshan Hospital of Traditional Chinese Medicine, Suzhou, Kunshan 215300, People's Republic of China.

Enzyme-linked immunosorbent assay (ELISA) is one of the most common methods in biological studies, and enzyme-linked immunospot (ELISpot) is a method to measure specific cell numbers by detecting protein secretion at a single-cell level. However, these two current methods can only detect one signal at one time and the sensitivity is not high enough to test low-concentration samples, which are major shortcomings in systematically analyzing the samples of interest. Herein, we demonstrated fluorescence-based oligo-linked immunosorbent assay (FOLISA) and fluorescence-based oligo-linked immunospot (FOLISPOT), which utilized DNA-barcoded antibodies to provide a highly multiplexed method with signal amplification. Signal amplification and simultaneous multiple-target detection were achieved by DNA complementary pairing and modular orthogonal DNA concatemers. By comparing FOLISA with traditional ELISA and comparing FOLISPOT with traditional ELISPOT, we found that the detection sensitivities of FOLISA and FOLISPOT are much higher than those of traditional ELISA and ELISPOT. The detection limit of ELISA is around 3 pg/mL, and the detection limit of FOLISA is below 0.06 pg/mL. FOLISPOT can detect more spots than ELISPOT and can detect targets that are undetectable for ELISPOT. Furthermore, FOLISA and FOLISPOT allowed sequential detection of multiple targets by using a single dye or multiple dyes in one round and sequential detection in multiple rounds. Thus, FOLISA and FOLISPOT enabled simultaneous detection of a large number of targets, significantly improved the detection sensitivity, and overcame the shortcomings of ELISA and ELISPOT. Overall, FOLISA and FOLISPOT presented effective and general platforms for rapid and multiplexed detection of antigens or antibodies with high sensitivity, either in laboratory tests or potentially in clinic tests.
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http://dx.doi.org/10.1021/acs.analchem.2c01087DOI Listing
June 2022

New Spiro-Sesquiterpenoids from the Marine Sponge Myrmekioderma sp.

Chem Biodivers 2022 Jun 1:e202200455. Epub 2022 Jun 1.

Research Center for Marine Drugs, Department of Pharmacy, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.

Three new spiro-sesquiterpenoids, myrmekiones A-C (1-3), were isolated from the marine sponge Myrmekioderma sp. collected from the South China Sea. The structures of 1-3 were experimentally illuminated though comprehensive NMR spectra, X-ray diffraction analysis and calculated ECD. These three compounds possessed a special spiro skeleton. Compound 1 was characterized by a chamigrane-type structure, it is the first time to obtain the single-crystal of this type of oil compounds. 2 and 3 were a pair of diastereoisomers that possessed an acorane skeleton. This study expands the chemical diversity of marine origin spiro-metabolites.
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http://dx.doi.org/10.1002/cbdv.202200455DOI Listing
June 2022

Signature Based on Six Autophagy-related Genes to Predict Prognosis of Head and Neck Squamous Cell Carcinoma.

Curr Med Sci 2022 Jun 1;42(3):597-605. Epub 2022 Jun 1.

Department of Health Statistics, Public Health School of Tianjin Medical University, Tianjin, 300070, China.

Objective: The present study aimed to develop an autophagy-related gene prognostic prediction model to provide survival risk prediction for head and neck squamous cell carcinoma (HNSCC) patients.

Methods: The K-mean cluster analysis was performed on HNSCC samples based on the expression values of 210 autophagy-related genes for candidate signature gene selection. LASSO Cox regression analysis was generated using the potential genes and the risk score was calculated from the prognosis model. The risk score was processed as an independent prognostic indicator to construct the nomogram model. The immune status including immune cell infiltration ratio and checkpoints of patients with HNSCC in high- and low-risk groups was also explored.

Results: LASSO Cox regression analysis was performed on the selected autophagy-related genes. According to the lambda value corresponding to the number of different genes in the LASSO Cox analysis, six genes (GABARAPL2, SAR1A, ST13, GAPDH, FADD and LAMP1) were finally chosen. The risk score based on the genes was generated, which was an independent prognostic marker for HNSCC. The prognostic prediction model (nomogram) was further optimized by the independent prognostic factors (risk score), which can better predict the prognosis and survival of patients. With the risk score and prognosis model, eight types of immune cells and six key immune checkpoints (CTLA4, PD1, IDO1, TDO2, LAG3, TIGIT) displayed expression specificity.

Conclusion: This study identified several potential prognostic biomarkers and established an autophagy-related prognostic prediction model for HNSCC, which provides a valuable reference for future clinical research.
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http://dx.doi.org/10.1007/s11596-022-2560-1DOI Listing
June 2022

Calcium-Related Gene Signatures May Predict Prognosis and Level of Immunosuppression in Gliomas.

Front Oncol 2022 13;12:708272. Epub 2022 May 13.

Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China.

Gliomas are the most common primary brain cancer. While it has been known that calcium-related genes correlate with gliomagenesis, the relationship between calcium-related genes and glioma prognosis remains unclear. We assessed TCGA datasets of mRNA expressions with differentially expressed genes (DEGs) and enrichment analysis to specifically screen for genes that regulate or are affected by calcium levels. We then correlated the identified calcium-related genes with unsupervised/supervised learning to classify glioma patients into 2 risk groups. We also correlated our identified genes with immune signatures. As a result, we discovered 460 calcium genes and 35 calcium key genes that were associated with OS. There were 13 DEGs between Clusters 1 and 2 with different OS. At the same time, 10 calcium hub genes (CHGs) signature model were constructed using supervised learning, and the prognostic risk scores of the 3 cohorts of samples were calculated. The risk score was confirmed as an independent predictor of prognosis. Immune enrichment analysis revealed an immunosuppressive tumor microenvironment with upregulation of checkpoint markers in the high-risk group. Finally, a nomogram was generated with risk scores and other clinical prognostic independent indicators to quantify prognosis. Our findings suggest that calcium-related gene expression patterns could be applicable to predict prognosis and predict levels of immunosuppression.
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http://dx.doi.org/10.3389/fonc.2022.708272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136236PMC
May 2022
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