Publications by authors named "Liang Wu"

703 Publications

Enhanced Metal-Insulator Transition in Freestanding VO Down to 5 nm Thickness.

ACS Appl Mater Interfaces 2021 Apr 1. Epub 2021 Apr 1.

Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, 637371, Singapore.

Ultrathin freestanding membranes with a pronounced metal-insulator transition (MIT) have huge potential for future flexible electronic applications as well as provide a unique aspect for the study of lattice-electron interplay. However, the reduction of the thickness to an ultrathin region (a few nm) is typically detrimental to the MIT in epitaxial films, and even catastrophic for their freestanding form. Here, we report an enhanced MIT in VO-based freestanding membranes, with a lateral size up to millimeters and the VO thickness down to 5 nm. The VO membranes were detached by dissolving a SrAlO sacrificial layer between the VO thin film and the -AlO(0001) substrate, allowing the transfer onto arbitrary surfaces. Furthermore, the MIT in the VO membrane was greatly enhanced by inserting an intermediate AlO buffer layer. In comparison with the best available ultrathin VO membranes, the enhancement of MIT is over 400% at a 5 nm VO thickness and more than 1 order of magnitude for VO above 10 nm. Our study widens the spectrum of functionality in ultrathin and large-scale membranes and enables the potential integration of MIT into flexible electronics and photonics.
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http://dx.doi.org/10.1021/acsami.1c01581DOI Listing
April 2021

Single-Cell Transcriptome Analysis Identifies Ligand-Receptor Pairs Associated With BCP-ALL Prognosis.

Front Oncol 2021 10;11:639013. Epub 2021 Mar 10.

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine, Shanghai Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

B cell precursor acute lymphoblastic leukemia (BCP-ALL) is a blood cancer that originates from the abnormal proliferation of B-lymphoid progenitors. Cell population components and cell-cell interaction in the bone marrow microenvironment are significant factors for progression, relapse, and therapy resistance of BCP-ALL. In this study, we identified specifically expressed genes in B cells and myeloid cells by analyzing single-cell RNA sequencing data for seven BCP-ALL samples and four healthy samples obtained from a public database. Integrating 1356 bulk RNA sequencing samples from a public database and our previous study, we found a total of 57 significant ligand-receptor pairs (24 upregulated and 33 downregulated) in the autocrine crosstalk network of B cells. Via assessment of the communication between B cells and myeloid cells, another 29 ligand-receptor pairs were discovered, some of which notably affected survival outcomes. A score-based model was constructed with least absolute shrinkage and selection operator (LASSO) using these ligand-receptor pairs. Patients with higher scores had poorer prognoses. This model can be applied to create predictions for both pediatric and adult BCP-ALL patients.
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http://dx.doi.org/10.3389/fonc.2021.639013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987943PMC
March 2021

Alkoxide hydrolysis in-situ constructing robust trimanganese tetraoxide/graphene composite for high-performance lithium storage.

J Colloid Interface Sci 2021 Mar 15;594:531-539. Epub 2021 Mar 15.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, 122 Luoshi Road, Wuhan 430070, Hubei, China; Laboratory of Inorganic Materials Chemistry (CMI), University of Namur, 61 rue de Bruxelles, Namur B-5000, Belgium. Electronic address:

Herein we develop a novel and effective alkoxide hydrolysis approach to in-situ construct the trimanganese tetraoxide (MnO)/graphene nanostructured composite as high-performance anode material for lithium-ion batteries (LIBs). This is the first report on the synthesis of MnO/graphene composite via a facile hydrolysis of the manganese alkoxide (Mn-alkoxide)/graphene precursor. Before hydrolysis, two dimensional (2D) Mn-alkoxide nanoplates are closely adhered to 2D graphene nanosheets via Mn-O chemical bonding. After hydrolysis, the Mn-alkoxide in-situ converts to MnO, while the Mn-O bond is preserved. This leads to a robust MnO/graphene hybrid architecture with 15 nm MnO nanocrystals homogeneously anchoring on graphene nanosheets. This not only prevents the MnO nanocrystals agglomeration but also inversely mitigates the graphene nanosheets restacking. Moreover, the flexible and conductive graphene nanosheets can accommodate the volume change. This maintains the structural and electrical integrity of the MnO/graphene electrode during the cycling process. As a result, the MnO/graphene composite displays superior lithium storage performance with high reversible capacity (741 mAh g at 100 mA g), excellent rate capability (403 mAh g at 1000 mA g) and long cycle life (527 mAg g after 300 cycles at 500 mA g). The electrochemical performance highlights the importance of rational design nanocrystals anchoring on graphene nanosheets for high-performance LIBs application.
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http://dx.doi.org/10.1016/j.jcis.2021.03.032DOI Listing
March 2021

Impact of the Paraglottic Space on Voice Production in an MRI-Based Vocal Fold Model.

J Voice 2021 Mar 19. Epub 2021 Mar 19.

Department of Head and Neck Surgery, University of California, Los Angeles, California. Electronic address:

Objective: While the vocal fold is in direct contact anteriorly with the thyroid cartilage, posteriorly the vocal fold connects to the thyroid cartilage through a soft tissue layer in the paraglottic space. Currently the paraglottic space is often neglected in computational models of phonation, in which a fixed boundary condition is often imposed on the lateral surface of the vocal fold. The goal of this study was to investigate the effect of the paraglottic space on voice production in an MRI-based vocal fold model, and how this effect may be counteracted by vocal fold stiffening due to laryngeal muscle activation.

Methods: Parametric simulation study using an MRI-based computational vocal fold model.

Results: The results showed that the presence of the paraglottic space increased the mean and amplitude of the glottal area waveform, decreased the phonation frequency and closed quotient. For the particular vocal fold geometry used in this study, the presence of the paraglottic space also reduced the occurrence of irregular vocal fold vibration. These effects of the paraglottic space became smaller with increasing paraglottic space stiffness and to a lesser degree with vocal fold stiffening.

Conclusions: The results suggest that the paraglottic space may be neglected in qualitative evaluations of normal phonation, but needs to be included in simulations of pathological phonation or vocal fold posturing.
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http://dx.doi.org/10.1016/j.jvoice.2021.02.021DOI Listing
March 2021

CytoTree: an R/Bioconductor package for analysis and visualization of flow and mass cytometry data.

BMC Bioinformatics 2021 Mar 22;22(1):138. Epub 2021 Mar 22.

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 197 Ruijin Er Road, Shanghai, 200025, China.

Background: The rapidly increasing dimensionality and throughput of flow and mass cytometry data necessitate new bioinformatics tools for analysis and interpretation, and the recently emerging single-cell-based algorithms provide a powerful strategy to meet this challenge.

Results: Here, we present CytoTree, an R/Bioconductor package designed to analyze and interpret multidimensional flow and mass cytometry data. CytoTree provides multiple computational functionalities that integrate most of the commonly used techniques in unsupervised clustering and dimensionality reduction and, more importantly, support the construction of a tree-shaped trajectory based on the minimum spanning tree algorithm. A graph-based algorithm is also implemented to estimate the pseudotime and infer intermediate-state cells. We apply CytoTree to several examples of mass cytometry and time-course flow cytometry data on heterogeneity-based cytology and differentiation/reprogramming experiments to illustrate the practical utility achieved in a fast and convenient manner.

Conclusions: CytoTree represents a versatile tool for analyzing multidimensional flow and mass cytometry data and to producing heuristic results for trajectory construction and pseudotime estimation in an integrated workflow.
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http://dx.doi.org/10.1186/s12859-021-04054-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983272PMC
March 2021

The Effects of Intensive Supervision Mechanism on Air Quality Improvement in China.

J Air Waste Manag Assoc 2021 Mar 19. Epub 2021 Mar 19.

Nankai University.

The Intensive Supervision Mechanism (hereafter referred to as ISM) is one of the most important institutional management innovations for air pollution control in China, but there is currently no consensus on the effects of the ISM on air quality improvement. In this study, a reliable quantitative model based on the Difference-in-Differences (DID) analysis was designed to evaluate the impacts of ISM on air quality (as indicated by good air quality days (hereafter referred to as GAD) and the concentrations of six major air pollutants (i.e. PM, PM, O_8H, NO, SO, and CO)), in China with focuses on the implementation cities of Henan Province. To optimize the model design, six meteorological factors, five socio-economic indicators, and VIIRS (Visible Infrared Imaging Radiometer Suite) data were also considered as alternative control variables for more comprehensive and effective results. In addition, the redundancy analysis (RDA) and Monte Carlo simulation were conducted to determine the optimal combination of those control variables which can best reflect the changes in explanatory variables. The main findings are as follows: (1) the statistical model applied in this study can well evaluate the impacts of ISM; (2) the implementation of ISM can significantly reduce the concentrations of SO CO, and NO, but the improvements for PM, PM, GAD and O_8H were not significant. (3) the potential for air quality improvement due to ISM tends to be reduced over time, and thus the positive effects of ISM at its second stage were not increased significantly compared with those observed during its first stage. In general, those results not only demonstrate the effectiveness of ISM on air quality improvement, but also provide insights into how the ISM can be optimized to gain a sustained improvement of the ambient air quality in the future.
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http://dx.doi.org/10.1080/10962247.2021.1906354DOI Listing
March 2021

Urinary soluble CD90 predicts renal prognosis in patients with diabetic kidney disease.

Ann Transl Med 2021 Feb;9(4):282

Renal Division, Department of Medicine, Peking University First Hospital, Beijing, China.

Background: Diabetic kidney disease (DKD), the major cause of chronic kidney disease, is associated with progressive renal fibrosis. The expression of CD90 correlated with fibrogenesis. However, the association between urinary soluble CD90 and renal disease severity, and whether it predicts outcomes in patients with DKD are still unclear.

Methods: Urinary sCD90 was measured in 285 patients with DKD in a longitudinal cohort. The composite endpoint was defined as end-stage renal disease (ESRD) or 40% reduction of estimated glomerular filtration rate (eGFR). The associations between urinary sCD90/Cr and clinical parameters, as well as renal outcomes were evaluated. Moreover, we detected the intrarenal CD90 expression, and demonstrated the correlation of intrarenal CD90 with clinico-pathological parameters.

Results: The urinary sCD90 level of DKD patients is significantly higher than diabetes patients without kidney injuries and healthy controls. We further showed urinary sCD90/Cr had significantly correlations with eGFR (r=-0.373, P<0.001), uACR (r=0.303, P<0.001), serum creatinine (r=0.344, P<0.001), and the eGFR slope (r=-0.27, P<0.001). Elevated urinary sCD90/Cr was an independent risk factor for the composite endpoint, adjustment for potential confounders in DKD patients (HR 1.20, 95% CI: 1.04-1.38, P=0.015). However, the CD90 expression in the renal tubulointerstitial compartment in DKD patients was significantly lower than healthy controls, and showed significant negative correlations with the interstitial fibrosis and tubular atrophy score (IFTA) (r=-0.3, P=0.047), and urinary sCD90/Cr (r=-0.399, P=0.029).

Conclusions: This study provided evidence that urinary sCD90 could reflect the disease severity and serve as a valuable factor for renal outcome prediction in patients with DKD.
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http://dx.doi.org/10.21037/atm-20-6528DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944307PMC
February 2021

Antituberculosis Drugs (Rifampicin and Isoniazid) Induce Liver Injury by Regulating NLRP3 Inflammasomes.

Mediators Inflamm 2021 19;2021:8086253. Epub 2021 Feb 19.

Nanchong Key Laboratory of Individualized Drug Therapy, Nanchong, Sichuan, China.

Patients being treated for pulmonary tuberculosis often suffer liver injury due to the effects of anti-TB drugs, and the underlying mechanisms for those injuries need to be clarified. In this study, rats and hepatic cells were administrated isoniazid (INH) and rifampin (RIF) and then treated with NLRP3-inflammasome inhibitors (INF39 and CP-456773) or NLRP3 siRNA. Histopathological changes that occurred in liver tissue were examined by H&E staining. Additionally, the levels IL-33, IL-18, IL-1, NLRP3, ASC, and cleaved-caspase 1 expression in the liver tissues were also determined. NAT2 and CYP2E1 expression were identified by QRT-PCR analysis. Finally, assays were performed to examine the effects of siRNA targeting NLRP3. Treatment with the antituberculosis drugs caused significant liver injuries, induced inflammatory responses and oxidative stress (OS), activated NLRP3 inflammasomes, reduced the activity of drug-metabolizing enzymes, and altered the antioxidant defense system in rats and hepatic cells. The NLRP3 inflammasome was required for INH- and RIF-induced liver injuries that were produced by inflammatory responses, OS, the antioxidant defense system, and drug-metabolizing enzymes. This study indicated that the NLRP3 inflammasome is involved in antituberculosis drug-induced liver injuries (ATLIs) and suggests NLRP3 as a potential target for attenuating the inflammation response in ATLIs.
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http://dx.doi.org/10.1155/2021/8086253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7914095PMC
February 2021

Exercise-related blood pressure response is related to autonomic modulation in young adults: A new extension study.

Technol Health Care 2021 ;29(S1):367-376

Background: The delayed blood pressure recovery (BPR) at post-exercise has been in association with a major risk of cardiovascular disease and death.

Objective: The study focused on evaluating the systolic and diastolic blood pressure recovery (SBPR, DBPR) and the autonomic modulation following treadmill exercise in healthy young adults. Although considerable literature had been published about BPR and HRV, the association between BPR and ultra-short-term HRV has not yet been completely described.

Methods: Fifteen subjects performed exercise with three different intensities on a treadmill, the speed was 6 km/h, 9 km/h, 12 km/h, respectively. SBP and DBP was measured per 30 s in each trial. The synchronous 5-min electrocardiogram (ECG) signals were recorded and HRV30s parameters including SDNN30s, RMSSD30s, SDNN30s/RMSSD30s, SD130s, SD230s and SD130s/SD230s were calculated every 30 s periods in 5 min ECG signals to match the corresponding BPR.

Results: The intraclass correlation coefficient (ICC) values and the Bland-Altman plots indicated good consistency and repeatability between HRV30s and HRV5min at three post-exercise trials, with most ICC values > 0.75. Besides, SBPR and DBPR generally decreased and returned to the Rest level in 5 mins. The Spearman correlation coefficients showed strong relationships between BPR and HRV30s sympathetic-vagal balance parameters, i.e., ratio SDNN/RMSSD and ratio SD1/SD2.

Conclusions: These observations represented a new insight into the cardiovascular regulation at post-exercise, which could contribute to physical exercise areas in the future.
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http://dx.doi.org/10.3233/THC-218035DOI Listing
January 2021

Annexin A1 alleviates kidney injury by promoting the resolution of inflammation in diabetic nephropathy.

Kidney Int 2021 Mar 3. Epub 2021 Mar 3.

The Institute of Cardiovascular Sciences, Institute of Systems Biomedicine, School of Basic Medical Sciences; Key Laboratory of Molecular Cardiovascular Science, Ministry of Education; Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Ministry of Health; Beijing Key Laboratory of Cardiovascular Receptors Research; Peking University Health Science Center; Beijing 100191, China; China National Clinical Research Center for Neurological Diseases, Tiantan Hospital, Advanced Innovation Center for Human Brain Protection, The Capital Medical University, Beijing 100050, China.. Electronic address:

Since failed resolution of inflammation is a major contributor to the progression of diabetic nephropathy, identifying endogenously generated molecules that promote the physiological resolution of inflammation may be a promising therapeutic approach for this disease. Annexin A1 (ANXA1), as an endogenous mediator, plays an important role in resolving inflammation. Whether ANXA1 could affect established diabetic nephropathy through modulating inflammatory states remains largely unknown. In the current study, we found that in patients with diabetic nephropathy, the levels of ANXA1 were upregulated in kidneys, and correlated with kidney function as well as kidney outcomes. Therefore, the role of endogenous ANXA1 in mouse models of diabetic nephropathy was further evaluated. ANXA1 deficiency exacerbated kidney injuries, exhibiting more severe albuminuria, mesangial matrix expansion, tubulointerstitial lesions, kidney inflammation and fibrosis in high fat diet/streptozotocin-induced-diabetic mice. Consistently, ANXA1 overexpression ameliorated kidney injuries in mice with diabetic nephropathy. Additionally, we found Ac2-26 (an ANXA1 mimetic peptide) had therapeutic potential for alleviating kidney injuries in db/db mice and diabetic Anxa1 knockout mice. Mechanistic studies demonstrated that intracellular ANXA1 bound to the transcription factor NF-κB p65 subunit, inhibiting its activation thereby modulating the inflammatory state. Thus, our data indicate that ANXA1 may be a promising therapeutic approach to treating and reversing diabetic nephropathy.
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http://dx.doi.org/10.1016/j.kint.2021.02.025DOI Listing
March 2021

Single-cell differential splicing analysis reveals high heterogeneity of liver tumor-infiltrating T cells.

Sci Rep 2021 Mar 5;11(1):5325. Epub 2021 Mar 5.

BGI-Shenzhen, Beishan Industrial Zone, Shenzhen, 518083, China.

Recent advances in single-cell RNA sequencing (scRNA-seq) have improved our understanding of the association between tumor-infiltrating lymphocyte (TILs) heterogeneity and cancer initiation and progression. However, studies investigating alternative splicing (AS) as an important regulatory factor of heterogeneity remain limited. Here, we developed a new computational tool, DESJ-detection, which accurately detects differentially expressed splicing junctions (DESJs) between cell groups at the single-cell level. We analyzed 5063 T cells of hepatocellular carcinoma (HCC) and identified 1176 DESJs across 11 T cell subtypes. Interestingly, DESJs were enriched in UTRs, and have putative effects on heterogeneity. Cell subtypes with a similar function closely clustered together at the AS level. Meanwhile, we identified a novel cell state, pre-activation with the isoform markers ARHGAP15-205. In summary, we present a comprehensive investigation of alternative splicing differences, which provided novel insights into T cell heterogeneity and can be applied to other full-length scRNA-seq datasets.
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http://dx.doi.org/10.1038/s41598-021-84693-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7935992PMC
March 2021

Three main short-chain fatty acids inhibit the activation of THP-1 cells by Mycoplasma pneumoniae.

Biosci Biotechnol Biochem 2021 Mar;85(4):923-930

School of Medicine, Jiangsu University, Zhenjiang, China.

The overactivation of macrophages causes chronic inflammatory diseases. Short-chain fatty acids (SCFAs), potential drugs for clinical treatment, are modulators of macrophage inflammatory reaction. Therefore, the modulation of macrophage-mediated cell activity is expected to become a new therapeutic strategy for inflammatory diseases caused by Mycoplasma pneumoniae. In this study, 2 kinds of SCFAs (propionate and butyrate) were found to have anti-inflammatory effects in M. pneumoniae-stimulated THP-1 cells inflammatory. They inhibited the expressions of IL-4, IL-6, ROS, and NLRP3 inflammasome, while enhancing the expressions of IL-10 and IFN-γ. Our study revealed these 2 agents to repress transcriptional activities of NF-κB, which are important modulators of inflammation. Meanwhile, SCFAs can significantly enhance the autophagy induced by M. pneumoniae. Considering that SCFAs have few side effects, they might be the promising adjuvant therapy for the prevention and/or treatment of various inflammatory diseases.
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http://dx.doi.org/10.1093/bbb/zbaa110DOI Listing
March 2021

Survival Factors and Metabolic Pathogenesis in Elderly Patients (≥65) With COVID-19: A Multi-Center Study.

Front Med (Lausanne) 2020 7;7:595503. Epub 2021 Jan 7.

Institute of Experimental Immunology, University Clinic of Rheinische Friedrich-Wilhelms-University, Bonn, Germany.

Elderly patients infected with COVID-19 are reported to be facing a substantially increased risk of mortality. Clinical characteristics, treatment options, and potential survival factors remain under investigation. This study aimed to fill this gap and provide clinically relevant factors associated with survival of elderly patients with COVID-19. In this multi-center study, elderly patients (age ≥65 years old) with laboratory-confirmed COVID-19 from 4 Wuhan hospitals were included. The clinical end point was hospital discharge or deceased with last date of follow-up on Jul. 08, 2020. Clinical, demographic, and laboratory data were collected. Univariate and multivariate analysis were performed to analyze survival and risk factors. A metabolic flux analysis using a large-scale molecular model was applied to investigate the pathogenesis of SARS-CoV-2 with regard to metabolism pathways. A total of 223 elderly patients infected with COVID-19 were included, 91 (40.8%) were discharged and 132 (59.2%) deceased. Acute respiratory distress syndrome (ARDS) developed in 140 (62.8%) patients, 23 (25.3%) of these patients survived. Multivariate analysis showed that potential risk factors for mortality were elevated D-Dimer (odds ratio: 1.13 [95% CI 1.04 - 1.22], = 0.005), high immune-related metabolic index (6.42 [95% CI 2.66-15.48], < 0.001), and increased neutrophil-to-lymphocyte ratio (1.08 [95% 1.03-1.13], < 0.001). Elderly patients receiving interferon atmotherapy showed an increased probability of survival (0.29 [95% CI 0.17-0.51], < 0.001). Based on these factors, an algorithm (AlgSurv) was developed to predict survival for elderly patients. The metabolic flux analysis showed that 12 metabolic pathways including phenylalanine (odds ratio: 28.27 [95% CI 10.56-75.72], < 0.001), fatty acid (15.61 [95% CI 6.66-36.6], < 0.001), and pyruvate (12.86 [95% CI 5.85-28.28], < 0.001) showed a consistently lower flux in the survivors vs. the deceased subgroup. This may reflect a key pathogenic mechanism of COVID-19 infection. Several factors such as interferon atmotherapy and recreased activity of specific metabolic pathways were found to be associated with survival of elderly patients. Based on these findings, a survival algorithm (AlgSurv) was developed to assist the clinical stratification for elderly patients. Dysregulation of the metabolic pathways revealed in this study may aid in the drug and vaccine development against COVID-19.
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http://dx.doi.org/10.3389/fmed.2020.595503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873923PMC
January 2021

Bacterial Quorum-Sensing Systems and Their Role in Intestinal Bacteria-Host Crosstalk.

Authors:
Liang Wu Yubin Luo

Front Microbiol 2021 28;12:611413. Epub 2021 Jan 28.

Department of Rheumatology and Immunology, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Institute of Immunology and Inflammation, Sichuan University, Chengdu, China.

Quorum-sensing (QS) system is a rapidly developing field in which we are gradually expanding our understanding about how bacteria communicate with each other and regulate their activities in bacterial sociality. In addition to collectively modifying bacterial behavior, QS-related autoinducers may also be embedded in the crosstalk between host and parasitic microbes. In this review, we summarize current studies on QS in the intestinal microbiome field and its potential role in maintaining homeostasis under physiological conditions. Additionally, we outline the canonical autoinducers and their related QS signal-response systems by which several pathogens interact with the host under pathological conditions, with the goal of better understanding intestinal bacterial sociality and facilitating novel antimicrobial therapeutic strategies.
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http://dx.doi.org/10.3389/fmicb.2021.611413DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876071PMC
January 2021

Different expression and localization of aquaporin 7 and aquaporin 9 in granulosa cells, oocytes, and embryos of patients with polycystic ovary syndrome and the negatively correlated relationship with insulin regulation.

Fertil Steril 2021 Feb;115(2):463-473

Center for Reproductive Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China. Electronic address:

Objective: To investigate the expression of aquaporin 7 (AQP7) and aquaporin 9 (AQP9) in the granulosa cells of patients with polycystic ovary syndrome (PCOS) and healthy women and detect their localization in oocytes at the germinal vesicle (GV), metaphase I (MI), MII, embryo, and blastocyst stages and the in vitro response to insulin stimulation.

Design: Randomized, assessor-blinded study.

Setting: Reproductive medical center.

Patient(s): A total of 40 women (aged 20-38 years) comprising 29 cases of primary infertility and 11 cases of secondary infertility, of whom 17 had an initial diagnosis of PCOS and three received a PCOS diagnosis after an infertility examination.

Intervention(s): Controlling different concentrations of insulin and different treatment times in cultures of normal human granulosa cells in vitro.

Main Outcome Measure(s): Expression of AQP7 and AQP9 genes and proteins in granulosa cells detected by real-time quantitative polymerase chain reaction, and localization in oocytes at the GV, MI, MII, embryo, and blastocyst stages by Western blot, immunohistochemical, and immunofluorescence assays, and concentrations of insulin in follicular fluid by enzyme-linked immunosorbent assay.

Result(s): The expression levels of the AQP7 mRNA and protein in the granulosa cells of patients with PCOS were higher than found in healthy controls. We found AQP7 protein expressed in human oocytes at GV, MI, MII, embryo, and blastocyst stages; it was mainly located in the nucleoplasm. In the PCOS group, the expression level of AQP9 mRNA and protein in granulosa cells was lower, and AQP9 protein was expressed in oocytes at the GV, MI, MII, embryo, and blastocyst stages; it was localized on the nuclear membrane. Compared with healthy women, the insulin expression in patients with PCOS was higher. In cultures of normal human granulosa cells in vitro, the expression of AQP7 and AQP9 mRNA and protein decreased with the increase in insulin concentration; expression statistically significantly decreased when the insulin concentration was 100 nmol/L, and after 6 to 24 hours of exposure the lowest expression levels were found at 12 hours.

Conclusion(s): The different localization and expression of AQP7 and AQP9 between the two groups suggests that they might be involved in oocyte maturation and embryonic development through different regulatory pathways. The expression levels of AQP7 and AQP9 were negatively correlated with insulin regulation, suggesting that insulin might affect the maturation of PCOS follicles by changing AQP7 and AQP9 expression.
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http://dx.doi.org/10.1016/j.fertnstert.2020.08.015DOI Listing
February 2021

3D Printed Biomimetic PCL Scaffold as Framework Interspersed With Collagen for Long Segment Tracheal Replacement.

Front Cell Dev Biol 2021 21;9:629796. Epub 2021 Jan 21.

Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

The rapid development of tissue engineering technology has provided new methods for tracheal replacement. However, none of the previously developed biomimetic tracheas exhibit both the anatomy (separated-ring structure) and mechanical behavior (radial rigidity and longitudinal flexibility) mimicking those of native trachea, which greatly restricts their clinical application. Herein, we proposed a biomimetic scaffold with a separated-ring structure: a polycaprolactone (PCL) scaffold with a ring-hollow alternating structure was three-dimensionally printed as a framework, and collagen sponge was embedded in the hollows amid the PCL rings by pouring followed by lyophilization. The biomimetic scaffold exhibited bionic radial rigidity based on compressive tests and longitudinal flexibility based on three-point bending tests. Furthermore, the biomimetic scaffold was recolonized by chondrocytes and developed tracheal cartilage . experiments showed substantial deposition of tracheal cartilage and formation of a biomimetic trachea mimicking the native trachea both structurally and mechanically. Finally, a long-segment tracheal replacement experiment in a rabbit model showed that the engineered biomimetic trachea elicited a satisfactory repair outcome. These results highlight the advantage of a biomimetic trachea with a separated-ring structure that mimics the native trachea both structurally and mechanically and demonstrates its promise in repairing long-segment tracheal defects.
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http://dx.doi.org/10.3389/fcell.2021.629796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859529PMC
January 2021

Hand-Held Zoom Micro-Imaging System Based on Microfluidic Chip for Point-of-Care Testing (POCT) of Vaginal Inflammation.

IEEE J Transl Eng Health Med 2021 26;9:2800109. Epub 2021 Jan 26.

Department of Food and Biological EngineeringJiangsu UniversityZhenjiang212013China.

Background: Vaginitis is a common and very private disease, and the current diagnosis is a frequent go to the hospital for testing.

Objective: In order to improve the convenience and speed of detection, in this paper, we have developed a hand-held zoom micro-imaging system based on a microfluidic chip for point-of-care testing (POCT) of vaginal inflammation.

Methods: This system consists of a microfluidic chip, an optical system and a hand-held zoom system assembled with a mobile phone. In terms of hardware, we designed a self-priming microfluidic chip, which can realize automatic sampling and full mixing of samples. We have also developed an optical system that can be adapted to smartphones, which has a lens group with a 37x magnification function and equipped with a zoom system with a focus range of 4mm to 6mm. In terms of software, we proposed an APP that can accurately identify cocci and can determine the inflammation level.

Results: Compared with the recognition rate of the observers in the hospital, the detection accuracy of the portable recognition system is 95%, and after testing the clinical samples, the results were completely consistent with the hospital diagnosis results. The detection limit was 500 CFU / ml, which the relative error was (0.9 ± 0.3) %, and recognition time is 7 seconds.

Conclusion: This system is definitely suitable for women's point-of-care testing (POCT).
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http://dx.doi.org/10.1109/JTEHM.2021.3054556DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861347PMC
January 2021

Clinical observation and analysis on the significance of quick sequential organ failure assessment in 74 non-ICU patients with sepsis.

Ann Palliat Med 2021 Jan;10(1):244-249

Department of Infectious Diseases, the Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Email:

Background: Sepsis is an important disease that endangers human health and is the main cause of death in ICU patients, which has been a focus of clinical treatment. This study aims to evaluate the significance of the readily available quick sequential organ failure assessment (qSOFA) score in clinical cases of sepsis.

Methods: A retrospective cross-sectional study of patients with sepsis treated in the Department of Infectious Diseases, the Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology from January 2015 to December 2016 was conducted, and the patients were divided into a high-score group (≥2 points) and the low-score group (<2 points) according to the diagnostic criteria for sepsis (Sepsis 3.0). The differences in disease outcome and inflammatory indicators were compared between groups.

Results: A total of 74 patients with sepsis were included in this study. When the cutoff qSOFA score was 2 points, the patients in the high-score group showed a higher mortality rate (71.43%), and the patients in the low-score group showed a higher improvement rate (87.76%). The inflammatory indicators did not show statistically significant differences between the two groups.

Conclusions: The qSOFA score can better predict the prognoses of non-ICU patients with sepsis compared with traditional inflammatory indicators. Clinicians should raise their awareness about qSOFA and improving its accuracy.
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http://dx.doi.org/10.21037/apm-20-2310DOI Listing
January 2021

Modulation of Local Overactive Inflammation via Injectable Hydrogel Microspheres.

Nano Lett 2021 Mar 5;21(6):2690-2698. Epub 2021 Feb 5.

Department of Orthopedic Surgery, Orthopedic Institute, The First Affiliated Hospital of Soochow University, 708 Renmin Road, Suzhou, Jiangsu 215006, P.R. China.

Although injectable hydrogel microsphere has demonstrated tremendous promise in clinical applications, local overactive inflammation in degenerative diseases could jeopardize biomaterial implantation's therapeutic efficacy. Herein, an injectable "peptide-cell-hydrogel" microsphere was constructed by covalently coupling of APETx2 and further loading of nucleus pulposus cells, which could inhibit local inflammatory cytokine storms to regulate the metabolic balance of ECM . The covalent coupling of APETx2 preserved the biocompatibility of the microspheres and achieved a controlled release of APETx2 for more than 28 days in an acidic environment. By delivering "peptide-cell-hydrogel" microspheres to a rat degenerative intervertebral disc at 4 weeks, the expression of ASIC-3 and IL-1β was significantly decreased for 3.53-fold and 7.29-fold, respectively. Also, the content of ECM was significantly recovered at 8 weeks. In summary, the proposed strategy provides an effective approach for tissue regeneration under overactive inflammatory responses.
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http://dx.doi.org/10.1021/acs.nanolett.0c04713DOI Listing
March 2021

Corrosion protection properties of different inhibitors containing PEO/LDHs composite coating on magnesium alloy AZ31.

Sci Rep 2021 Feb 2;11(1):2774. Epub 2021 Feb 2.

College of Materials Science and Engineering, Chongqing University, Chongqing, 400044, China.

Corrosion inhibitors 2,5-pyridinedicarboxilate (PDC), sodium metavanadate (SMV) and 5-aminosalicylate (AS) were impregnated into porous PEO coatings respectively via vacuuming process, followed by fast sealing treatment in a Ce containing solution. After that layered double hydroxides (LDHs) based nanocontainers were respectively prepared on them via hydrothermal treatment. In frame of this work it was shown, that sealing effect for the pore was provided by formation of new phase CeO on the surface of PEO coatings. And, hydrothermal preparation for preparing LDHs leaded obvious changes in structure and thickness of the coatings. In addition, impregnation of inhibitors was in favor of improving LDHs content in final composite coatings. EIS result indicated that AS/Ce-HT specimen exhibited a best corrosion protection.
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http://dx.doi.org/10.1038/s41598-021-81029-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854598PMC
February 2021

Polydatin inhibits LPS-induced inflammatory response in BV2 microglia by disrupting the formation of lipid rafts.

Immunopharmacol Immunotoxicol 2021 Apr 28;43(2):138-144. Epub 2021 Jan 28.

Department of Neurosurgery, General Hospital of Ningxia Medical University, Yinchuan, China.

Objective: Polydatin has been used in the treatment of various inflammatory diseases. However, its role in the regulation of neuroinflammation has not been reported. In this study, we designed to investigate the anti-inflammatory effects of polydatin in LPS-stimulated BV2 microglia cells.

Methods: Inflammatory mediators TNF-α, IL-1β, NO, and PGE2 production were measured by ELISA. The protein of signaling pathways were detected by western blot analysis.

Results: The results showed that polydatin significantly ameliorated the production of TNF-α, IL-1β, NO, and PGE2 up-regulated by LPS. Polydatin also blocked LPS-induced NF-κB activation. In addition, PI3K and AKT, the up-stream molecules of NF-κB signaling pathway, were inhibited by the treatment of polydatin. Also, we found the formation of lipid rafts was inhibited by polydatin through attenuating the cholesterol content. Finally, polydatin was found to increase the expression of ABCA1 and ABCG1.

Conclusion: In conclusion, the results of the present study suggested that polydatin exhibited its anti-inflammatory effects in BV2 cells through disrupting lipid rafts, which subsequently inhibiting PI3K/AKT signaling pathway.
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http://dx.doi.org/10.1080/08923973.2020.1867999DOI Listing
April 2021

Multiregion single-cell sequencing reveals the transcriptional landscape of the immune microenvironment of colorectal cancer.

Clin Transl Med 2021 Jan;11(1):e253

Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, School of Medicine, South China University of Technology, Guangzhou, Guangdong, China.

The tumor microenvironment is a complex ecosystem formed by distinct and interacting cell populations, and its composition is related to cancer prognosis and response to clinical treatment. In this study, we have taken the advantage of two single-cell RNA sequencing technologies (Smart-seq2 and DNBelab C4) to generate an atlas of 15,115 immune and nonimmune cells from primary tumors and hepatic metastases of 18 colorectal cancer (CRC) patients. We observed extensive changes in the proportions and functional states of T cells and B cells in tumor tissues, compared to those of paired non-tumor tissues. Importantly, we found that B cells from early CRC tumor were identified to be pre-B like expressing tumor suppressors, whereas B cells from advanced CRC tumors tended to be developed into plasma cells. We also identified the association of IgA IGLC2 plasma cells with poor CRC prognosis, and demonstrated a significant interaction between B-cell and myeloid-cell signaling, and found CCL8 cycling B cells/CCR5 T-cell interactions as a potential antitumoral mechanism in advanced CRC tumors. Our results provide deeper insights into the immune infiltration within CRC, and a new perspective for the future research in immunotherapies for CRC.
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http://dx.doi.org/10.1002/ctm2.253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775989PMC
January 2021

Giant topological longitudinal circular photo-galvanic effect in the chiral multifold semimetal CoSi.

Nat Commun 2021 Jan 8;12(1):154. Epub 2021 Jan 8.

Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, PA, 19104, USA.

The absence of mirror symmetry, or chirality, is behind striking natural phenomena found in systems as diverse as DNA and crystalline solids. A remarkable example occurs when chiral semimetals with topologically protected band degeneracies are illuminated with circularly polarized light. Under the right conditions, the part of the generated photocurrent that switches sign upon reversal of the light's polarization, known as the circular photo-galvanic effect, is predicted to depend only on fundamental constants. The conditions to observe quantization are non-universal, and depend on material parameters and the incident frequency. In this work, we perform terahertz emission spectroscopy with tunable photon energy from 0.2 -1.1 eV in the chiral topological semimetal CoSi. We identify a large longitudinal photocurrent peaked at 0.4 eV reaching  ~550 μ A/V, which is much larger than the photocurrent in any chiral crystal reported in the literature. Using first-principles calculations we establish that the peak originates only from topological band crossings, reaching 3.3 ± 0.3 in units of the quantization constant. Our calculations indicate that the quantized circular photo-galvanic effect is within reach in CoSi upon doping and increase of the hot-carrier lifetime. The large photo-conductivity suggests that topological semimetals could potentially be used as novel mid-infrared detectors.
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http://dx.doi.org/10.1038/s41467-020-20408-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794406PMC
January 2021

Effect of early oxygen therapy and antiviral treatment on disease progression in patients with COVID-19: A retrospective study of medical charts in China.

PLoS Negl Trop Dis 2021 01 6;15(1):e0009051. Epub 2021 Jan 6.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Until now, no antiviral treatment has been proven to be effective for the coronavirus disease 2019 (COVID-19). The timing of oxygen therapy was considered to have a great influence on the symptomatic relief of hypoxemia and seeking medical intervention, especially in situations with insufficient medical resources, but the evidence on the timing of oxygen therapy is limited.

Methods And Findings: Medical charts review was carried out to collect the data of hospitalized patients with COVID-19 infection confirmed in Tongji hospital, Wuhan from 30th December 2019 to 8th March 2020. In this study, the appropriate timing of oxygen therapy and risk factors associated with severe and fatal illness were identified and the effectiveness of antivirus on disease progression was assessed. Among 1362 patients, the prevalence of hypoxia symptoms was significantly higher in those patients with severe and fatal illness than in those with less severe disease. The onset of hypoxia symptoms was most common in the second to third week after symptom onset, and patients with critical and fatal illness experienced these symptoms earlier than those with mild and severe illness. In multivariable analyses, the risk of death increased significantly when oxygen therapy was started more than 2 days after hypoxia symptoms onset among critical patients (OR, 1.92; 95%CI, 1.20 to 3.10). Compared to the critically ill patients without IFN-a, the patients who were treated with IFN-a had a lower mortality (OR, 0.60; 95%CI, 0.39 to 0.91).

Conclusions: Early initiation of oxygen therapy was associated with lower mortality among critical patients. This study highlighted the importance of early oxygen therapy after the onset of hypoxia symptoms. Our results also lend support to potentially beneficial effects of IFNα on critical illness.
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http://dx.doi.org/10.1371/journal.pntd.0009051DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7815117PMC
January 2021

Emerging molecular subtypes and therapeutic targets in B-cell precursor acute lymphoblastic leukemia.

Front Med 2021 Jan 5. Epub 2021 Jan 5.

Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.

B-cell precursor acute lymphoblastic leukemia (BCP-ALL) is characterized by genetic alterations with high heterogeneity. Precise subtypes with distinct genomic and/or gene expression patterns have been recently revealed using high-throughput sequencing technology. Most of these profiles are associated with recurrent non-overlapping rearrangements or hotspot point mutations that are analogous to the established subtypes, such as DUX4 rearrangements, MEF2D rearrangements, ZNF384/ZNF362 rearrangements, NUTM1 rearrangements, BCL2/MYC and/or BCL6 rearrangements, ETV6-RUNX1-like gene expression, PAX5alt (diverse PAX5 alterations, including rearrangements, intragenic amplifications, or mutations), and hotspot mutations PAX5 (p.Pro80Arg) with biallelic PAX5 alterations, IKZF1 (p.Asn159Tyr), and ZEB2 (p.His1038Arg). These molecular subtypes could be classified by gene expression patterns with RNA-seq technology. Refined molecular classification greatly improved the treatment strategy. Multiagent therapy regimens, including target inhibitors (e.g., imatinib), immunomodulators, monoclonal antibodies, and chimeric antigen receptor T-cell (CAR-T) therapy, are transforming the clinical practice from chemotherapy drugs to personalized medicine in the field of risk-directed disease management. We provide an update on our knowledge of emerging molecular subtypes and therapeutic targets in BCP-ALL.
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http://dx.doi.org/10.1007/s11684-020-0821-6DOI Listing
January 2021

Shielded goethite catalyst that enables fast water dissociation in bipolar membranes.

Nat Commun 2021 Jan 4;12(1). Epub 2021 Jan 4.

CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Centre of Chemistry for Energy Materials, Department of Applied Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, 230026, Hefei, China.

Optimal pH conditions for efficient artificial photosynthesis, hydrogen/oxygen evolution reactions, and photoreduction of carbon dioxide are now successfully achievable with catalytic bipolar membranes-integrated water dissociation and in-situ acid-base generations. However, inefficiency and instability are severe issues in state-of-the-art membranes, which need to urgently resolve with systematic membrane designs and innovative, inexpensive junctional catalysts. Here we show a shielding and in-situ formation strategy of fully-interconnected earth-abundant goethite FeO(OH) catalyst, which lowers the activation energy barrier from 5.15 to 1.06 eV per HO - H bond and fabricates energy-efficient, cost-effective, and durable shielded catalytic bipolar membranes. Small water dissociation voltages at limiting current density (U: 0.8 V) and 100 mA cm (U: 1.1 V), outstanding cyclic stability at 637 mA cm, long-time electro-stability, and fast acid-base generations (HSO: 3.9 ± 0.19 and NaOH: 4.4 ± 0.21 M m min at 100 mA cm) infer confident potential use of the novel bipolar membranes in emerging sustainable technologies.
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http://dx.doi.org/10.1038/s41467-020-20131-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782813PMC
January 2021

PLAGL2 promotes the proliferation and migration of gastric cancer cells via USP37-mediated deubiquitination of Snail1.

Theranostics 2021 1;11(2):700-714. Epub 2021 Jan 1.

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Road No. 1277, Wuhan 430022, China.

PLAGL2 (pleomorphic adenoma gene like-2), a zinc finger PLAG transcription factor, is aberrantly expressed in several malignant tumors. However, the biological roles of PLAGL2 and its underlying mechanism in gastric cancer (GC) remain unclear. A series of experiments and were conducted to reveal the role of PLAGL2 in GC progression. The data revealed that PLAGL2 promotes GC cell proliferation, migration, invasion, and EMT and . Mechanistically, we demonstrated the critical role of PLAGL2 in the stabilization of snail family transcriptional repressor 1 (Snail1) and promoting Snail1-mediated proliferation and migration of GC cells. PLAGL2 activated the transcription of deubiquitinase USP37, which then interacted with and deubiquitinated Snail1 protein directly. In addition, GSK-3β-dependent phosphorylation of Snail1 protein is essential for USP37-mediated Snail1 deubiquitination regulation. In general, PLAGL2 promotes the proliferation and migration of GC cells through USP37-mediated deubiquitination of Snail1 protein. This work provided potential therapeutic targets for GC treatment.
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http://dx.doi.org/10.7150/thno.47800DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738862PMC
January 2021

Development and validation of prognostic model for predicting mortality of COVID-19 patients in Wuhan, China.

Sci Rep 2020 12 31;10(1):22451. Epub 2020 Dec 31.

Institute of Experimental Immunology, University Clinic of Rheinische Friedrich-Wilhelms-University, Bonn, Germany.

Novel coronavirus 2019 (COVID-19) infection is a global public health issue, that has now affected more than 200 countries worldwide and caused a second wave of pandemic. Severe adult respiratory syndrome-CoV-2 (SARS-CoV-2) pneumonia is associated with a high risk of mortality. However, prognostic factors predicting poor clinical outcomes of individual patients with SARS-CoV-2 pneumonia remain under intensive investigation. We conducted a retrospective, multicenter study of patients with SARS-CoV-2 who were admitted to four hospitals in Wuhan, China from December 2019 to February 2020. Mortality at the end of the follow up period was the primary outcome. Factors predicting mortality were also assessed and a prognostic model was developed, calibrated and validated. The study included 492 patients with SARS-CoV-2 who were divided into three cohorts: the training cohort (n = 237), the validation cohort 1 (n = 120), and the validation cohort 2 (n = 135). Multivariate analysis showed that five clinical parameters were predictive of mortality at the end of follow up period, including advanced age [odds ratio (OR), 1.1/years increase (p < 0.001)], increased neutrophil-to-lymphocyte ratio [(NLR) OR, 1.14/increase (p < 0.001)], elevated body temperature on admission [OR, 1.53/°C increase (p = 0.005)], increased aspartate transaminase [OR, 2.47 (p = 0.019)], and decreased total protein [OR, 1.69 (p = 0.018)]. Furthermore, the prognostic model drawn from the training cohort was validated with validation cohorts 1 and 2 with comparable area under curves (AUC) at 0.912, 0.928, and 0.883, respectively. While individual survival probabilities were assessed, the model yielded a Harrell's C index of 0.758 for the training cohort, 0.762 for the validation cohort 1, and 0.711 for the validation cohort 2, which were comparable among each other. A validated prognostic model was developed to assist in determining the clinical prognosis for SARS-CoV-2 pneumonia. Using this established model, individual patients categorized in the high risk group were associated with an increased risk of mortality, whereas patients predicted to be in the low risk group had a higher probability of survival.
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http://dx.doi.org/10.1038/s41598-020-78870-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7775455PMC
December 2020

Bronchial Sleeve Resection with Complete Pulmonary Preservation: A Single-Center Experience.

Cancer Manag Res 2020 16;12:12975-12982. Epub 2020 Dec 16.

Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Shanghai, People's Republic of China.

Purpose: Bronchial sleeve resection with complete pulmonary preservation (BSRCPP) is a classic surgical method for the treatment of benign or low-grade bronchial tumors. For elderly patients and patients with poor cardiopulmonary function, BSRCPP is particularly advantageous because some of these patients may not tolerate lobectomy or pneumonectomy. We retrospectively reviewed the clinical data of 20 patients who underwent BSRCPP during the past 7 years. This report presents the experience with BSRCPP in our department.

Patients And Methods: We collected the data of 20 patients who underwent BSRCPP. Of these 20 patients, 17 underwent thoracotomy and 3 underwent video-assisted thoracoscopic surgery (VATS). The study cohort comprised 7 male and 13 female patients with an average age of 44 years (range, 4-71 years). All patients underwent a systematic preoperative examination to confirm the surgical indications and methods. Regular follow-up was conducted after the operation.

Results: All patients survived and remained clinically well. Two of the 20 patients (10%) were re-admitted to the hospital because of pulmonary air leakage, which was resolved after thoracic drainage. No patients developed tumor recurrence.

Conclusion: BSRCPP may be an effective treatment for selected patients with bronchial tumors. Notably, however, many technical key points require improvement, especially in VATS. Therefore, thoracoscopic minimally invasive treatment requires more practice and exploration.
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http://dx.doi.org/10.2147/CMAR.S286934DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7751305PMC
December 2020

Single-cell landscape of the ecosystem in early-relapse hepatocellular carcinoma.

Cell 2021 Jan 23;184(2):404-421.e16. Epub 2020 Dec 23.

Department of Liver Surgery & Transplantation, Liver Cancer Institute, Zhongshan Hospital, Fudan University, and Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai 200032, China; Zhong-Hua Precision Medical Center, Zhongshan Hospital, Fudan University-BGI, Shanghai 200032, China. Electronic address:

Hepatocellular carcinoma (HCC) has high relapse and low 5-year survival rates. Single-cell profiling in relapsed HCC may aid in the design of effective anticancer therapies, including immunotherapies. We profiled the transcriptomes of ∼17,000 cells from 18 primary or early-relapse HCC cases. Early-relapse tumors have reduced levels of regulatory T cells, increased dendritic cells (DCs), and increased infiltrated CD8 T cells, compared with primary tumors, in two independent cohorts. Remarkably, CD8 T cells in recurrent tumors overexpressed KLRB1 (CD161) and displayed an innate-like low cytotoxic state, with low clonal expansion, unlike the classical exhausted state observed in primary HCC. The enrichment of these cells was associated with a worse prognosis. Differential gene expression and interaction analyses revealed potential immune evasion mechanisms in recurrent tumor cells that dampen DC antigen presentation and recruit innate-like CD8 T cells. Our comprehensive picture of the HCC ecosystem provides deeper insights into immune evasion mechanisms associated with tumor relapse.
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http://dx.doi.org/10.1016/j.cell.2020.11.041DOI Listing
January 2021