Publications by authors named "Liang Lin"

306 Publications

Deductive Reinforcement Learning for Visual Autonomous Urban Driving Navigation.

IEEE Trans Neural Netw Learn Syst 2021 Sep 14;PP. Epub 2021 Sep 14.

Existing deep reinforcement learning (RL) are devoted to research applications on video games, e.g., The Open Racing Car Simulator (TORCS) and Atari games. However, it remains under-explored for vision-based autonomous urban driving navigation (VB-AUDN). VB-AUDN requires a sophisticated agent working safely in structured, changing, and unpredictable environments; otherwise, inappropriate operations may lead to irreversible or catastrophic damages. In this work, we propose a deductive RL (DeRL) to address this challenge. A deduction reasoner (DR) is introduced to endow the agent with ability to foresee the future and to promote policy learning. Specifically, DR first predicts future transitions through a parameterized environment model. Then, DR conducts self-assessment at the predicted trajectory to perceive the consequences of current policy resulting in a more reliable decision-making process. Additionally, a semantic encoder module (SEM) is designed to extract compact driving representation from the raw images, which is robust to the changes of the environment. Extensive experimental results demonstrate that DeRL outperforms the state-of-the-art model-free RL approaches on the public CAR Learning to Act (CARLA) benchmark and presents a superior performance on success rate and driving safety for goal-directed navigation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TNNLS.2021.3109284DOI Listing
September 2021

An increase of phosphatidylcholines in follicular fluid implies attenuation of embryo quality on day 3 post-fertilization.

BMC Biol 2021 Sep 9;19(1):200. Epub 2021 Sep 9.

College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China.

Background: Although oocyte quality is the dominant factor determining embryo quality, few studies have been conducted to evaluate embryo quality based on the metabolites related to the oocyte. With quantification of the follicular fluid (FF) metabolites, in assisted reproductive technology (ART), this study sought to evaluate the embryo or oocyte quality through an informative approach.

Results: An evaluation model consisting of 17 features was generated to distinguish the embryo quality on day 3 post-fertilization, and phosphatidylcholines (PCs) were the key contributors to the evaluation. The model was extended to the patients under different ages and hyperstimulations, and the features were further enriched to facilitate the evaluation of the embryo quality. The metabolites were clustered through pathway analysis, leading to a hypothesis that accumulation of arachidonic acid induced by PCs might weaken embryo quality on day 3 post-fertilization.

Conclusions: A discriminating model with metabolic features elicited from follicular fluid was established, which enabled the evaluation of the embryo or oocyte quality even under certain clinical conditions, and the increase of PCs in follicular fluid implies the attenuation of embryo quality on day 3 post-fertilization.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12915-021-01118-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428131PMC
September 2021

Learning Spatially Variant Linear Representation Models for Joint Filtering.

IEEE Trans Pattern Anal Mach Intell 2021 Aug 6;PP. Epub 2021 Aug 6.

Joint filtering mainly uses an additional guidance image as a prior and transfers its structures to the target image in the filtering process. Different from existing approaches that rely on local linear models or hand-designed objective functions to extract the structural information from the guidance image, we propose a new joint filtering method based on a spatially variant linear representation model (SVLRM), where the target image is linearly represented by the guidance image. However, the SVLRM leads to a highly ill-posed problem. To estimate the spatially variant linear representation coefficients, we develop an effective approach based on a deep convolutional neural network (CNN). As such, the proposed deep CNN (constrained by the SVLRM) is able to model the structural information of both the guidance and input images. We show that the proposed approach can be effectively applied to a variety of applications, including depth/RGB image upsampling and restoration, flash/no-flash image deblurring, natural image denoising, and scale-aware filtering. In addition, we show that the linear representation model can be extended to high-order representation models. Extensive experimental results demonstrate that the proposed method performs favorably against the state-of-the-art methods that have been specifically designed for each task.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TPAMI.2021.3102575DOI Listing
August 2021

A Universal Strategy toward the Precise Regulation of Initial Coulombic Efficiency of Li-Rich Mn-Based Cathode Materials.

Adv Mater 2021 Sep 1;33(38):e2103173. Epub 2021 Aug 1.

State Key Laboratory of Physical Chemistry of Solid Surface Fujian Key Laboratory of Materials Genome Collaborative Innovation Center of Chemistry for Energy Materials College of Materials, Xiamen University, Xiamen, 361005, China.

Li-rich Mn-based cathode materials (LRMs) are potential cathode materials for high energy density lithium-ion batteries. However, low initial Coulombic efficiency (ICE) severely hinders the commercialization of LRM. Herein, a facile oleic acid-assisted interface engineering is put forward to precisely control the ICE, enhance reversible capacity and rate performance of LRM effectively. As a result, the ICE of LRM can be precisely adjusted from 84.1% to 100.7%, and a very high specific capacity of 330 mAh g at 0.1 C, as well as outstanding rate capability with a fascinating specific capacity of 250 mAh g at 5 C, are harvested. Theoretical calculations reveal that the introduced cation/anion double defects can reduce the diffusion barrier of Li ions, and in situ surface reconstruction layer can induce a self-built-in electric field to stabilize the surface lattice oxygen. Moreover, this facile interface engineering is universal and can enhance the ICEs of other kinds of LRM effectively. This work provides a valuable new idea for improving the comprehensive electrochemical performance of LRM through multistrategy collaborative interface engineering technology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202103173DOI Listing
September 2021

Pathogenicity Analysis of Weaned Piglets Challenged With Novel Emerging Senecavirus A in Fujian, China.

Front Vet Sci 2021 7;8:694110. Epub 2021 Jul 7.

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

In order to evaluate the pathogenicity of Senecavirus A (SVA) to weaned piglets preliminarily, 28-day-old weaned piglets were challenged with SVA by intramuscular injection. The clinical manifestations, antibody levels, and tissue viral load of infected piglets were detected. The results indicated that the piglets challenged with SVA CH/FuJ/2017 showed drowsiness, lameness, oral blisters, diarrhea, and other clinical signs. Lesions on the hooves were observed. Red spots or plaques were initially observed on the hoof and then developed into blisters that cracked and gradually formed scab. The symptoms and signs were relieved after 8 days post-infection (dpi). The sentinel piglet, feeding together with the challenged piglets, showed similar clinical signs with the challenged piglets after 3 dpi. Monitoring of antibody levels showed that anti-SVA antibody could be detected at 5 dpi by competition enzyme-linked immunosorbent assay (cELISA) method, and neutralizing antibody could be detected after 7 dpi. Analysis of viral tissue distribution and viral load indicated that SVA could replicate in the liver, spleen, lung, kidney, and lymph node. In all, Senecavirus disease was successfully replicated by SVA CH/FuJ/2017 isolate, which verified the clinical manifestations of SVA infection in weaned piglets, and provided a foundation for further SVA pathogenesis and vaccine development.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fvets.2021.694110DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292739PMC
July 2021

BCMA-Specific ADC MEDI2228 and Daratumumab Induce Synergistic Myeloma Cytotoxicity via IFN-Driven Immune Responses and Enhanced CD38 Expression.

Clin Cancer Res 2021 Jul 23. Epub 2021 Jul 23.

Jerome Lipper Multiple Myeloma Center, LeBow Institute for Myeloma Therapeutics, Dana-Farber Cancer Institute, Harvard Medical School, Boston Massachusetts.

Purpose: Efforts are required to improve the potency and durability of CD38- and BCMA-based immunotherapies in human multiple myeloma. We here delineated the molecular and cellular mechanisms underlying novel immunomodulatory effects triggered by BCMA pyrrolobenzodiazepine (PBD) antibody drug conjugate (ADC) MEDI2228 which can augment efficacy of these immunotherapies.

Experimental Design: MEDI2228-induced transcriptional and protein changes were investigated to define significantly impacted genes and signaling cascades in multiple myeloma cells. Mechanisms whereby MEDI2228 combination therapies can enhance cytotoxicity or overcome drug resistance in multiple myeloma cell lines and patient multiple myeloma cells were defined using models of tumor in the bone marrow (BM) microenvironment, as well as in human natural killer (NK)-reconstituted NOD/SCID gamma (NSG) mice bearing MM1S tumors.

Results: MEDI2228 enriched IFN I signaling and enhanced expression of IFN-stimulated genes in multiple myeloma cell lines following the induction of DNA damage-ATM/ATR-CHK1/2 pathways. It activated cGAS-STING-TBK1-IRF3 and STAT1-IRF1-signaling cascades and increased CD38 expression in multiple myeloma cells but did not increase CD38 expression in BCMA-negative NK effector cells. It overcame CD38 downregulation on multiple myeloma cells triggered by IL6 and patient BM stromal cell-culture supernatant via activation of STAT1-IRF1, even in immunomodulatory drug (IMiD)- and bortezomib-resistant multiple myeloma cells. and upregulation of NKG2D ligands and CD38 in MEDI2228-treated multiple myeloma cells was further associated with synergistic daratumumab (Dara) CD38 MoAb-triggered NK-mediated cytotoxicity of both cell lines and autologous drug-resistant patient multiple myeloma cells.

Conclusions: These results provide the basis for clinical evaluation of combination MEDI2228 with Dara to further improve patient outcome in multiple myeloma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1158/1078-0432.CCR-21-1621DOI Listing
July 2021

Sum frequency generation spectroscopy of the attachment disc of a spider.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Dec 9;263:120161. Epub 2021 Jul 9.

School of Electronic and Computer Science, University of Southampton, Southampton, SO17 1BJ, UK.

The pyriform silk of the attachment disc of a spider was studied using infrared-visible vibrational sum frequency generation (SFG) spectroscopy. The spider can attach dragline and radial lines to many kinds of substrates in nature (concrete, alloy, metal, glass, plant branches, leaves, etc.) with the attachment disc. The adhesion can bear the spider's own weight, and resist the wind on its orb web. From our SFG spectroscopy study, the NH group of arginine side chain and/or NH group of arginine and glutamine side chain in the amino acid sequence of the attachment silk proteins are suggested to be oriented in the disc. It was inferred from the observed doublet SFG peaks at around 3300 cm that the oriented peptide contains two kinds of structures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.saa.2021.120161DOI Listing
December 2021

Dynamics and inhibition of MLL1 CXXC domain on DNA revealed by single-molecule quantification.

Biophys J 2021 08 17;120(16):3283-3291. Epub 2021 Jul 17.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Tianjin, China. Electronic address:

CpG islands recruit MLL1 via the CXXC domain to modulate chromatin structure and regulate gene expression. The amino acid motif of CXXC also plays a pivotal role in MLL1's structure and function and serves as a target for drug design. In addition, the CpG pattern in an island governs spatially dependent collaboration among CpGs in recruiting epigenetic enzymes. However, current studies using short DNA fragments cannot probe the dynamics of CXXC on long DNA with crowded CpG motifs. Here, we used single-molecule magnetic tweezers to examine the binding dynamics of MLL1's CXXC domain on a long DNA with a CpG island. The mechanical strand separation assay allows profiling of protein-DNA complexes and reports force-dependent unfolding times. Further design of a hairpin detector reveals the unfolding time of individual CXXC-CpG complexes. Finally, in a proof of concept we demonstrate the inhibiting effect of dimethyl fumarate on the CXXC-DNA complexes by measuring the dose response curve of the unfolding time. This demonstrates the potential feasibility of using single-molecule strand separation as a label-free detector in drug discovery and chemical biology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bpj.2021.03.045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8392069PMC
August 2021

Off-Pump Aortic Bypass for Hypoplastic Arch and Aortic Coarctation Via Anterolateral Thoracotomy.

World J Pediatr Congenit Heart Surg 2021 Jul;12(4):557-558

Department of Cardiac Surgery, 12667Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

We report the case of a 21-year-old woman who was referred with uncontrolled hypertension. Computed tomography angiography revealed aortic arch hypoplasia and severe aortic coarctation. An off-pump ascending-to-descending aortic bypass surgery using synthetic graft was performed via left anterolateral thoracotomy. The patient recovered well and was discharged home uneventfully after five days. This procedure was performed without touching the head vessels or any collateral vessels. We consider this a safe and less invasive alternative technique for adult coarctation patients who have aortic hypoplasia or interrupted aorta.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/21501351211003533DOI Listing
July 2021

Retreat for Advancing: Dynamic Reinforced Instruction Attacker for Robust Visual Navigation.

IEEE Trans Pattern Anal Mach Intell 2021 Jul 16;PP. Epub 2021 Jul 16.

Language instruction plays an essential role in the natural language grounded navigation tasks. However, navigators trained with limited human-annotated instructions may have difficulties in accurately capturing key information from the complicated instruction at different timesteps, leading to poor navigation performance. In this paper, we exploit to train a more robust navigator which is capable of dynamically extracting crucial factors from the long instruction, by using an adversarial attacking paradigm. Specifically, we propose a Dynamic Reinforced Instruction Attacker (DR-Attacker), which learns to mislead the navigator to move to the wrong target by destroying the most instructive information in instructions at different timesteps. By formulating the perturbation generation as a Markov Decision Process, DR-Attacker is optimized by the reinforcement learning algorithm to generate perturbed instructions sequentially during the navigation, according to a learnable attack score. Then, the perturbed instructions, which serve as hard samples, are used for improving the robustness of the navigator with an effective adversarial training strategy and an auxiliary self-supervised reasoning task. Experimental results on both Vision-and-Language Navigation (VLN) and Navigation from Dialog History (NDH) tasks show the superiority of our proposed method over state-of-the-art methods. Moreover, the visualization analysis shows the effectiveness of the proposed DR-Attacker, which can successfully attack crucial information in the instructions at different timesteps.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TPAMI.2021.3097435DOI Listing
July 2021

Prognostic factors and treatment of neuroendocrine tumors of the uterine cervix based on the FIGO 2018 staging system: a single-institution study of 172 patients.

PeerJ 2021 6;9:e11563. Epub 2021 Jul 6.

Department of Gynecological-Surgical Oncology, Fujian Medical University Cancer Hospital, Fujian Cancer Hospital, Fuzhou, Fujian, China.

Objective: This study aimed to explore the prognostic factors and outcomes of patients with neuroendocrine tumors (NETs) of the cervix and to determine appropriate treatment.

Methods: A single-institution retrospective analysis of 172 patients with NETs was performed based on the new International Federation of Gynecology and Obstetrics (FIGO 2018) staging system.

Results: Among the 172 eligible patients, 161 were diagnosed with small cell neuroendocrine carcinoma (SCNEC), six with large cell neuroendocrine carcinoma, four with typical carcinoid tumors and one with SCNEC combined with an atypical carcinoid tumor. According to the FIGO 2018 staging guidelines, 31 were stage I, 66 were stage II, 57 were stage III, and 18 were stage IV. The 5-year survival rates of patients with stage I-IV disease were 74.8%, 56.2%, 41.4% and 0%, respectively. The 5-year progression-free survival rates of patients with stage I-IV disease were 63.8%, 54.5%, 30.8% and 0%, respectively. In the multivariate analysis, advanced FIGO stage, large tumor and older age were identified as independent variables for 5-year survival in patients with stage I-IV disease. FIGO stage, tumor size and para-aortic lymph node metastasis were independent prognostic factors for 5-year progression-free survival in patients with stage I-IV disease. For the patients receiving surgery ( = 108), tumor size and pelvic lymph node metastasis were independent prognostic factors for 5-year survival, and pelvic lymph node metastasis for 5-year progression-free survival. In stage IVB, at least six cycles of chemotherapy ( = 7) was associated with significantly better 2-year OS (83.3% vs. 9.1%, < 0.001) and 2-year PFS (57.1% vs. 0%, = 0.01) than fewer than six cycles of chemotherapy( = 11).

Conclusion: Advanced FIGO stage, large tumor, older age and lymph node metastasis are independent prognostic factors for NETs of the cervix. The TP/TC and EP regimens were the most commonly used regimens, with similar efficacies and toxicities. Standardized and complete multimodality treatment may improve the survival of patients with NETs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.11563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269661PMC
July 2021

Seroprevalence of Chlamydia abortus infection in yak (Bos grunniens) in Tibet, China.

Ir Vet J 2021 Jun 30;74(1):19. Epub 2021 Jun 30.

College of Veterinary Medicine, Northwest A&F University, 712100, Yangling, China.

Chlamydia spp. are prevalent zoonotic pathogens that infect a wide variety of host species. Chlamydia abortus (C. abortus) infection in yaks has been reported in Gansu and Qinghai province, China. However, no data about C. abortus infection are available in yaks in Tibet, China. A total of 938 serum samples was collected from yaks in Tibet, China and specific antibodies against C. abortus were detected by the enzyme-linked immunosorbent assay (ELISA). The results showed that the overall seroprevalence of C. abortus in yaks was 104/938 (11.1 %, 95 % confidence interval [CI] 9.1-13.1). The prevalence in female and male yaks was 59/556 (10.6 %, 95 % CI 8.0-13.2) and 45/382 (11.8 %, 95 % CI 8.5-15.0), respectively with no significant difference (p > 0.05). The seroprevalence of antibodies to C. abortus in yaks ranged from 8.0 to 18.2 % among the six different areas, and the difference was also without statistical significance (p > 0.05). The prevalence among different age groups ranged from 7.0 to 15.9 %, with a higher prevalence among 1 to 2 years age category. The results demonstrate the presence of C. abortus infection in yaks in Tibet and may pose a risk for the general yak populations in addition to its potential impact on public health and the local Tibetan economy. To our knowledge, this is the first seroprevalence survey of C. abortus in yaks in Tibet, China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13620-021-00199-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246664PMC
June 2021

Cloning and Functional Characterization of a Squalene Synthase from Paris polyphylla var. yunnanensis.

Chem Biodivers 2021 Jul 17;18(7):e2100342. Epub 2021 Jun 17.

State Key Laboratory of Phytochemistry and Plant Resources in West China, and Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, P. R. China.

Paris polyphylla Smith var. yunnanensis (Franch.) Hand. - Mazz. is a precious traditional Chinese medicine, and steroidal saponins are its major bioactive constituents possessing extensive biological activities. Squalene synthase (SQS) catalyzes the first dedicated step converting two molecular of farnesyl diphosphate (FDP) into squalene, a key intermediate in the biosynthetic pathway of steroidal saponins. In this study, a squalene synthase gene (PpSQS1) was cloned and functionally characterized from P. polyphylla var. yunnanensis, representing the first identified SQS from the genus Paris. The open reading frame of PpSQS1 is 1239 bp, which encodes a protein of 412 amino acids showing high similarity to those of other plant SQSs. Expression of PpSQS1 in Escherichia coli resulted in production of soluble recombinant proteins. Gas chromatography-mass spectrometry analysis showed that the purified recombinant PpSQS1 protein could produce squalene using FDP as a substrate in the in vitro enzymatic assay. qRT-PCR analysis indicated that PpSQS1 was highly expressed in rhizomes, consistent with the dominant accumulation of steroidal saponins there, suggesting that PpSQS1 is likely involved in the biosynthesis of steroidal saponins in the plant. The findings lay a foundation for further investigation on the biosynthesis and regulation of steroidal saponins, and also provide an alternative gene for manipulation of steroid production using synthetic biology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cbdv.202100342DOI Listing
July 2021

Semantics-Aware Adaptive Knowledge Distillation for Sensor-to-Vision Action Recognition.

IEEE Trans Image Process 2021 16;30:5573-5588. Epub 2021 Jun 16.

Existing vision-based action recognition is susceptible to occlusion and appearance variations, while wearable sensors can alleviate these challenges by capturing human motion with one-dimensional time-series signals (e.g. acceleration, gyroscope, and orientation). For the same action, the knowledge learned from vision sensors (videos or images) and wearable sensors, may be related and complementary. However, there exists a significantly large modality difference between action data captured by wearable-sensor and vision-sensor in data dimension, data distribution, and inherent information content. In this paper, we propose a novel framework, named Semantics-aware Adaptive Knowledge Distillation Networks (SAKDN), to enhance action recognition in vision-sensor modality (videos) by adaptively transferring and distilling the knowledge from multiple wearable sensors. The SAKDN uses multiple wearable-sensors as teacher modalities and uses RGB videos as student modalities. To preserve the local temporal relationship and facilitate employing visual deep learning models, we transform one-dimensional time-series signals of wearable sensors to two-dimensional images by designing a gramian angular field based virtual image generation model. Then, we introduce a novel Similarity-Preserving Adaptive Multi-modal Fusion Module (SPAMFM) to adaptively fuse intermediate representation knowledge from different teacher networks. Finally, to fully exploit and transfer the knowledge of multiple well-trained teacher networks to the student network, we propose a novel Graph-guided Semantically Discriminative Mapping (GSDM) module, which utilizes graph-guided ablation analysis to produce a good visual explanation to highlight the important regions across modalities and concurrently preserve the interrelations of original data. Experimental results on Berkeley-MHAD, UTD-MHAD, and MMAct datasets well demonstrate the effectiveness of our proposed SAKDN for adaptive knowledge transfer from wearable-sensors modalities to vision-sensors modalities. The code is publicly available at https://github.com/YangLiu9208/SAKDN.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TIP.2021.3086590DOI Listing
June 2021

Modulatory Effects of on the Performance, Morphology, Cecal Microbiota and Gut Barrier Function of Laying Hens.

Animals (Basel) 2021 May 24;11(6). Epub 2021 May 24.

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

We investigated the efficacy of a single bacterium strain, () YW1, on the performance, morphology, cecal microbiota, and intestinal barrier function of laying hens. A total of 216 28-week-old Hy-line Brown laying hens were divided into three dietary treatment groups, with six replicates of 12 birds each for 4 weeks. The control group (Ctr) was fed a basal diet and the treatment groups, T1 and T2, were fed a basal diet supplemented with at a dose rate of 5 × 10 CFU/kg and 2.5 × 10 CFU/kg, respectively. Dietary supplementation with did not significantly affect overall egg production in both groups, with no obvious changes in average egg weight and intestine morphology. administration also improved the physical barrier function of the intestine by inducing significantly greater expression levels of the tight junction protein occludin in T1 ( = 0.07) and T2 ( < 0.05). Further, supplementation with effectively modulated the cecal microbiota, increasing the relative level of beneficial bacteria at the genus level (e.g.,   < 0.05, = 0.298,   = 0.550) and decreasing the level of potential pathogens (e.g., < 0.05, < 0.05, = 0.298). Overall, YW1 supplementation cannot significantly improve the egg production; however, it modulated the cecal microbiota towards a healthier pattern and promoted the mRNA expression of the tight junction protein occludin in laying hens, making YW1 a good probiotic candidate for application in the poultry industry, and further expanding the resources of strains of animal probiotics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ani11061523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225007PMC
May 2021

Feature Extraction Using Sparse Kernel Non-Negative Matrix Factorization for Rolling Element Bearing Diagnosis.

Sensors (Basel) 2021 May 25;21(11). Epub 2021 May 25.

School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

For early fault detection of a bearing, the localized defect generally brings a complex vibration signal, so it is difficult to detect the periodic transient characteristics from the signal spectrum using conventional bearing fault diagnosis methods. Therefore, many matrix analysis technologies, such as singular value decomposition (SVD) and reweighted SVD (RSVD), were proposed recently to solve this problem. However, such technologies also face failure in bearing fault detection due to the poor interpretability of the obtained eigenvector. Non-negative Matrix Factorization (NMF), as a part-based representation algorithm, can extract low-rank basis spaces with natural sparsity from the time-frequency representation. It performs excellent interpretability of the factor matrices due to its non-negative constraints. By this virtue, NMF can extract the fault feature by separating the frequency bands of resonance regions from the amplitude spectrogram automatically. In this paper, a new feature extraction method based on sparse kernel NMF (KNMF) was proposed to extract the fault features from the amplitude spectrogram in greater depth. By decomposing the amplitude spectrogram using the kernel-based NMF model with L1 regularization, sparser spectral bases can be obtained. Using KNMF with the linear kernel function, the time-frequency distribution of the vibration signal can be decomposed into a subspace with different frequency bands. Thus, we can extract the fault features, a series of periodic impulses, from the decomposed subspace according to the sparse frequency bands in the spectral bases. As a result, the proposed method shows a very high performance in extracting fault features, which is verified by experimental investigations and benchmarked by the Fast Kurtogram, SVD and NMF-based methods.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/s21113680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198267PMC
May 2021

Adaptive parameter of standard deviation enhances the power of noninvasive prenatal screens.

J Matern Fetal Neonatal Med 2021 Apr 28:1-7. Epub 2021 Apr 28.

Beijing USCI Medical Laboratory, Beijing, China.

Purpose: Traditional test methods during noninvasive prenatal screens (NIPS) use the fixed parameter of standard deviation (SD), which ignores the influence of actual sequencing read counts of a sample on the results. The aim of this study is to eliminate the influence of the sequencing depth of individual samples on the results and enhance the power of NIPS.

Methods: In this study, we propose an improved NIPS method, which calculates the SD in the score process adaptively according to the actual read count of the test sample. Our approach obtained the SD linear fitting function along with the read count with a large number of reference samples, in which SD and read count fit well. The effectiveness of our enhanced NIPS method was evaluated on three common trisomy syndromes and five recurrent CNV syndromes with 3219 and 6592 samples based on whole genome sequencing of maternal peripheral blood.

Results: A total of 3,219 pregnant samples have been used for validating the proposed method on detecting fetal trisomy syndromes (T13, T18, and T21), in which eight false negative (FN) samples have been corrected as true positive (TP) and eight false positive (FP) samples have been fixed as true negative (TN) with our proposed adaptive-SD method. Another 6592 samples were used to compare the two methods on detecting five recurrent fetal copy number variation (CNV) syndromes, in which the FP samples have decreased from 99 to 39.

Conclusions: Our adaptive-SD NIPS method shows more power on detecting both trisomy syndromes and five recurrent CNVs in the pregnant samples with diverse read counts. Besides, our proposed method contributes to lower FP and FN samples than the traditional test method in NIPS. Our results show that our enhanced NIPS methods are effective in detecting both abnormal fetal trisomy syndromes and recurrent CNV syndromes in pregnant women.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/14767058.2021.1912001DOI Listing
April 2021

Modulatory Effects of Probiotics During Pathogenic Infections With Emphasis on Immune Regulation.

Front Immunol 2021 8;12:616713. Epub 2021 Apr 8.

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

In order to inhibit pathogenic complications and to enhance animal and poultry growth, antibiotics have been extensively used for many years. Antibiotics applications not only affect target pathogens but also intestinal beneficially microbes, inducing long-lasting changes in intestinal microbiota associated with diseases. The application of antibiotics also has many other side effects like, intestinal barrier dysfunction, antibiotics residues in foodstuffs, nephropathy, allergy, bone marrow toxicity, mutagenicity, reproductive disorders, hepatotoxicity carcinogenicity, and antibiotic-resistant bacteria, which greatly compromise the efficacy of antibiotics. Thus, the development of new antibiotics is necessary, while the search for antibiotic alternatives continues. Probiotics are considered the ideal antibiotic substitute; in recent years, probiotic research concerning their application during pathogenic infections in humans, aquaculture, poultry, and livestock industry, with emphasis on modulating the immune system of the host, has been attracting considerable interest. Hence, the adverse effects of antibiotics and remedial effects of probiotics during infectious diseases have become central points of focus among researchers. Probiotics are live microorganisms, and when given in adequate quantities, confer good health effects to the host through different mechanisms. Among them, the regulation of host immune response during pathogenic infections is one of the most important mechanisms. A number of studies have investigated different aspects of probiotics. In this review, we mainly summarize recent discoveries and discuss two important aspects: (1) the application of probiotics during pathogenic infections; and (2) their modulatory effects on the immune response of the host during infectious and non-infectious diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.616713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060567PMC
June 2021

Joint Learning of Neural Transfer and Architecture Adaptation for Image Recognition.

IEEE Trans Neural Netw Learn Syst 2021 Apr 19;PP. Epub 2021 Apr 19.

Current state-of-the-art visual recognition systems usually rely on the following pipeline: 1) pretraining a neural network on a large-scale data set (e.g., ImageNet) and 2) finetuning the network weights on a smaller, task-specific data set. Such a pipeline assumes that the sole weight adaptation is able to transfer the network capability from one domain to another domain based on a strong assumption that a fixed architecture is appropriate for all domains. However, each domain with a distinct recognition target may need different levels/paths of feature hierarchy, where some neurons may become redundant, and some others are reactivated to form new network structures. In this work, we prove that dynamically adapting network architectures tailored for each domain task along with weight finetuning benefits in both efficiency and effectiveness, compared to the existing image recognition pipeline that only tunes the weights regardless of the architecture. Our method can be easily generalized to an unsupervised paradigm by replacing supernet training with self-supervised learning in the source domain tasks and performing linear evaluation in the downstream tasks. This further improves the search efficiency of our method. Moreover, we also provide principled and empirical analysis to explain why our approach works by investigating the ineffectiveness of existing neural architecture search. We find that preserving the joint distribution of the network architecture and weights is of importance. This analysis not only benefits image recognition but also provides insights for crafting neural networks. Experiments on five representative image recognition tasks, such as person re-identification, age estimation, gender recognition, image classification, and unsupervised domain adaptation, demonstrate the effectiveness of our method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TNNLS.2021.3070605DOI Listing
April 2021

The efficacy of reduced-visit prenatal care model during the coronavirus disease 2019 pandemic: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Apr;100(15):e25435

Department of Obstetrics, General Hospital of the Central Theater Command of the Chinese People's Liberation Army, Wuhan, Hubei, China.

Background: While this reduced-visit prenatal care model during the COVID-19 pandemic is well-intentioned, there is still a lack of relevant evidence to prove its effectiveness. Therefore, in order to provide new evidence-based medical evidence for clinical treatment, we undertook a systematic review and meta-analysis to assess the efficacy of reduced-visit prenatal care model during the COVID-19 pandemic.

Methods: The online literature will be searched using the following combination of medical subject heading terms: "prenatal care" OR "prenatal nursing" AND "reduced-visit" OR "reduce visit" OR "virtual visit." MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials, and Web of Science will be searched without any language restrictions. A standard data extraction form is used independently by 2 reviewers to retrieve the relevant data from the articles. The outcome measures are as following: pregnancy-related stress, satisfaction with care, quality of care. The present study will be performed by Review Manager Software (RevMan Version 5.3, The Cochrane Collaboration, Copenhagen, Denmark). P < .05 is set as the significance level.

Results: It is hypothesized that reduced-visit prenatal care model will provide similar outcomes compared with traditional care model.

Conclusions: The results of our review will be reported strictly following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) criteria and the review will add to the existing literature by showing compelling evidence and improved guidance in clinic settings.

Osf Registration Number: 10.17605/OSF.IO/WYMB7.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000025435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052079PMC
April 2021

Aberrantly Expressed Galectin-9 Is Involved in the Immunopathogenesis of Anti-MDA5-Positive Dermatomyositis-Associated Interstitial Lung Disease.

Front Cell Dev Biol 2021 25;9:628128. Epub 2021 Mar 25.

Department of Rheumatology, Beijing Key Lab for Immune-Mediated Inflammatory Diseases, China-Japan Friendship Hospital, Beijing, China.

Background: Dermatomyositis (DM) associated rapidly progressive interstitial lung disease (RP-ILD) has high mortality rate and poor prognosis. Galectin-9 (Gal-9) plays multiple functions in immune regulation. We investigated Gal-9 expression in DM patients and its association with DM-ILD.

Methods: A total of 154 idiopathic inflammatory myopathy patients and 30 healthy controls were enrolled in the study. Cross-sectional and longitudinal studies were used to analyze the association between serum Gal-9 levels and clinical features. Enzyme-linked immunosorbent assay and qRT-PCR were used to examine Gal-9 expression in the sera and isolated peripheral blood mononuclear cells (PBMCs) from DM patients. Immunohistochemistry was performed to analyze the expression of Gal-9 and its ligand (T-cell immunoglobulin mucin (Tim)-3 and CD44) in lung tissues from anti-melanoma differentiation-associated gene 5 (MDA5)-positive patients. The effect of Gal-9 on human lung fibroblasts (MRC-5) was investigated .

Results: Serum Gal-9 levels were significantly higher in DM patients than in immune-mediated necrotizing myopathy patients and healthy controls (all < 0.001). Higher serum Gal-9 levels were observed in anti-MDA5-positive DM patients than in anti-MDA5-negative DM patients [33.8 (21.9-44.7) vs. 16.2 (10.0-26.9) ng/mL, < 0.001]. Among the anti-MDA5-positive DM patients, serum Gal-9 levels were associated with RP-ILD severity. Serum Gal-9 levels were significantly correlated with disease activity in anti-MDA5-positive DM patients in both cross-sectional and longitudinal studies. PBMCs isolated from anti-MDA5-positive DM patients (3.7 ± 2.3 ng/mL) produced higher levels of Gal-9 than those from immune-mediated necrotizing myopathy patients (1.1 ± 0.3 ng/mL, = 0.022) and healthy controls (1.4 ± 1.2 ng/mL, = 0.045). The mRNA levels of Gal-9 were positively correlated with the levels of type-I interferon-inducible genes MX1 ( = 0.659, = 0.020) and IFIH1 ( = 0.787, = 0.002) in PBMCs from anti-MDA5-positive DM patients. Immunohistochemistry revealed increased Gal-9 and Tim-3 expression in the lung tissues of patients with DM and RP-ILD. stimulation with Gal-9 protein increased CCL2 mRNA expression in MRC-5 fibroblasts.

Conclusions: Among anti-MDA5-positive DM patients, Gal-9 could be a promising biomarker for monitoring disease activity, particularly for RP-ILD severity. Aberrant expression of the Gal-9/Tim-3 axis may be involved in the immunopathogenesis of DM-ILD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcell.2021.628128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027128PMC
March 2021

Transcriptome and lipidome profile of human mesenchymal stem cells with reduced senescence and increased trilineage differentiation ability upon drug treatment.

Aging (Albany NY) 2021 03 26;13(7):9991-10014. Epub 2021 Mar 26.

School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, Heilongjiang, China.

Human Mesenchymal stem cells (hMSCs) are multi-potential cells which are widely used in cell therapy. However, the frequently emerged senescence and decrease of differentiation capabilities limited the broad applications of MSC. Several strategies such as small molecules treatment have been widely studied and used to improve the stem characteristics bypassing the senescence but the exact mechanisms for them to reduce senescence have not been fully studied. In this study, hMSCs were treated by rapamycin, oltipraz, metformin, and vitamin C for the indicated time and these cells were subjected to senescence evaluation and trilineage differentiation. Furthermore, transcriptomics and lipidomics datasets of hMSCs after drug treatment were analyzed to interpret biological pathways responsible for their anti-senescence effects. Although four drugs exhibited significant activities in promoting MSC osteogenic differentiation, metformin is the optimal drug to promote trilineage differentiation. GO terms illustrated that the anti-aging effects of drugs were mainly associated with cellular senescence, mitotic and meiosis process. Biosynthesis of phosphatidylcholines (PC) and phosphatidylethanolamine (PE) were inhibited whereas production of phosphatidylinositols (PIs) and saturated fatty acids (SFA)/ mono-unsaturated fatty acids (MUFA) conversion was activated. Medium free fatty acids (FFA) was increased in hMSCs with different anti-aging phenotypes. Therefore, we established a comprehensive method in assessing drug intervention based on the results of transcriptomics and lipidomics. The method can be used to study different biological phenotypes upon drug intervention in MSC which will extend the clinical application of hMSCs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/aging.202759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064146PMC
March 2021

An Optical Method for Immediate Evaluation of Microfoam Stability in Foam Sclerotherapy.

Skin Pharmacol Physiol 2021 1;34(3):128-134. Epub 2021 Apr 1.

School of Biological Science and Medical Engineering, Beihang University, Beijing, China.

Objectives: The objective of our study was to develop an optical method that instantly evaluates the stability of sclerosing foam, which would enable early predictions of the clinical performance of the foam and reduce the occurrence of clinical side effects.

Methods: Based on the principle of light scattering, we developed a method to optically test foam stability and verified it experimentally using sodium morrhuate (2 mL; 0.05 g/mL) and carbon dioxide. A self-made foam preparation instrument was used to achieve a preparation speed of 275 mm/s. The liquid-gas ratios were considered as 1:3, 1:4, and 1:5. Curves of illuminance with respect to the drainage rate and decay time were obtained. By fitting the curve, the relationship between foam half-life time (FHT) and foam decay was obtained. Thus, foam stability was evaluated using the initial illuminance value; the foam transfer time was approximately 3 s.

Results: The experimental FHT varies between 205 and 232 s. Illuminance is exponentially related to drainage rate and linearly related with time. FHT can be expressed by the initial illuminance and illuminance curve fitting coefficients. The half-life of the foam decreases as the initial illuminance value increases, for the same sclerosing drug. The suitability of foam stability is determined by the position of the initial value in the chart.

Conclusion: Optical methods are feasible for evaluating foam stability over a short period of time. Clinically predicting the stability of freshly prepared foam can reduce number of incidences of further complications. This will promote the development of foam sclerotherapy and provide a basic understanding of the internal mechanical properties of foam.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1159/000514322DOI Listing
April 2021

Challenges and Recent Advances in High Capacity Li-Rich Cathode Materials for High Energy Density Lithium-Ion Batteries.

Adv Mater 2021 Mar 26:e2005937. Epub 2021 Mar 26.

State Key Laboratory for Physical Chemistry of Solid Surfaces, Fujian Key Laboratory of Materials Genome, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Materials Science and Engineering, College of Materials, Xiamen University, Xiamen, 361005, P. R. China.

Li-rich cathode materials have attracted increasing attention because of their high reversible discharge capacity (>250 mA h g ), which originates from transition metal (TM) ion redox reactions and unconventional oxygen anion redox reactions. However, many issues need to be addressed before their practical applications, such as their low kinetic properties and inefficient voltage fading. The development of cutting-edge technologies has led to cognitive advances in theory and offer potential solutions to these problems. Herein, a recent in-depth understanding of the mechanisms and the frontier electrochemical research progress of Li-rich cathodes are reviewed. In addition, recent advances associated with various strategies to promote the performance and the development of modification methods are discussed. In particular, excluding Li-rich Mn-based (LRM) cathodes, other branches of the Li-rich cathode materials are also summarized. The consistent pursuit is to obtain energy storage devices with high capacity, reliable practicability, and absolute safety. The recent literature and ongoing efforts in this area are also described, which will create more opportunities and new ideas for the future development of Li-rich cathode materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202005937DOI Listing
March 2021

Development of a TaqMan loop-mediated isothermal amplification assay for the rapid detection of pigeon paramyxovirus type 1.

Arch Virol 2021 Jun 23;166(6):1599-1605. Epub 2021 Mar 23.

Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Pigeon paramyxovirus-1 (PPMV-1) is a strain of Newcastle disease virus (NDV) that has adapted to infect pigeons and poses a constant threat to the commercial poultry industry. Early detection via rapid and sensitive methods, along with timely preventative and mitigating actions, is important for reducing the spread of PPMV-1. Here, we report the development of a TaqMan loop-mediated isothermal amplification assay (TaqMan-LAMP) for rapid and specific detection of PPMV-1 based on the F gene. This system makes use of six novel primers and a TaqMan probe that targets nine distinct regions of the F gene that are highly conserved among PPMV-1 isolates. The results showed that the limit of detection was 10 copies μL for PPMV-1 cDNA and 0.1 ng for PPMV-1 RNA. The reaction was completed within 25 min and was thus faster than conventional RT-PCR. Moreover, no cross-reactions with similar viruses or with peste des petits ruminants virus (PPRV) or NDV LaSota vaccine strains were observed under the same conditions. To evaluate the applicability of the assay, the TaqMan-LAMP assay and a commercial RT-PCR assay were compared using 108 clinical samples, and the concordance rate between two methods was found to be 96.3%. The newly developed PPMV-1 TaqMan-LAMP assay can therefore be used for simple, efficient, rapid, specific, and sensitive diagnosis of PPMV-1 infections.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00705-021-04963-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7986176PMC
June 2021

Intralayered Ostwald Ripening-Induced Self-Catalyzed Growth of CNTs on MXene for Robust Lithium-Sulfur Batteries.

Small 2021 Apr 17;17(17):e2007446. Epub 2021 Mar 17.

Henan Key Laboratory of Photovoltaic Materials, Henan University, Kaifeng, 475004, China.

The distinguishable physicochemical properties of MXenes render them attractive in electrochemical energy storage. However, the strong tendency to self-restack owing to the van der Waals interactions between the MXene layers incurs a massive decrease in surface area and blocking of ions transfer and electrolytes penetration. Here, in situ generated Ti C T MXene-carbon nanotubes (Ti C T -CNTs) hybrids are reported via low-temperature self-catalyzing growth of CNTs on Ti C T nanosheets without the addition of any catalyst precursors. With combined spectroscopic studies and theoretical calculation results, it is certified that the intralayered Ostwald ripening-induced Ti C T nanomesh structure contributes to the uniform precipitation of ultrafine metal Ti catalysts on Ti C T , thus giving rise to the in situ CNTs formation on the surface of Ti C T with high integrity. Taking advantages of intimate electrolyte penetration, unobstructed 3D Li /e transport, and rich electroactive sites, the Ti C T -CNTs hybrids are confirmed to be ideal 3D scaffolds for accommodating sulfur and regulating the polysulfides conversion for high-loaded lithium-sulfur batteries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202007446DOI Listing
April 2021

Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation.

Int J Mol Sci 2021 Feb 22;22(4). Epub 2021 Feb 22.

Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, China.

Plant species conservation through cryopreservation using plant vitrification solutions (PVS) is based in empiricism and the mechanisms that confer cell integrity are not well understood. Using ESI-MS/MS analysis and quantification, we generated 12 comparative lipidomics datasets for membranes of embryogenic cells (ECs) of during cryogenic treatments. Each step of the complex PVS-based cryoprotocol had a profoundly different impact on membrane lipid composition. Loading treatment (osmoprotection) remodeled the cell membrane by lipid turnover, between increased phosphatidic acid (PA) and phosphatidylglycerol (PG) and decreased phosphatidylcholine (PC) and phosphatidylethanolamine (PE). The PA increase likely serves as an intermediate for adjustments in lipid metabolism to desiccation stress. Following PVS treatment, lipid levels increased, including PC and PE, and this effectively counteracted the potential for massive loss of lipid species when cryopreservation was implemented in the absence of cryoprotection. The present detailed cryobiotechnology findings suggest that the remodeling of membrane lipids and attenuation of lipid degradation are critical for the successful use of PVS. As lipid metabolism and composition varies with species, these new insights provide a framework for technology development for the preservation of other species at increasing risk of extinction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms22042174DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7926411PMC
February 2021

The Key Regulator of Necroptosis, RIP1 Kinase, Contributes to the Formation of Astrogliosis and Glial Scar in Ischemic Stroke.

Transl Stroke Res 2021 Feb 24. Epub 2021 Feb 24.

Jiangsu Key Laboratory of Neuropsychiatric Diseases and College of Pharmaceutical Sciences, Laboratory of Cerebrovascular Pharmacology, College of Pharmaceutical Science, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases, School of Public Health, Soochow University, 199 Ren-Ai Road, Suzhou, 215123, Jiangsu, China.

Necroptosis initiation relies on the receptor-interacting protein 1 kinase (RIP1K). We recently reported that genetic and pharmacological inhibition of RIP1K produces protection against ischemic stroke-induced astrocytic injury. However, the role of RIP1K in ischemic stroke-induced formation of astrogliosis and glial scar remains unknown. Here, in a transient middle cerebral artery occlusion (tMCAO) rat model and an oxygen and glucose deprivation and reoxygenation (OGD/Re)-induced astrocytic injury model, we show that RIP1K was significantly elevated in the reactive astrocytes. Knockdown of RIP1K or delayed administration of RIP1K inhibitor Nec-1 down-regulated the glial scar markers, improved ischemic stroke-induced necrotic morphology and neurologic deficits, and reduced the volume of brain atrophy. Moreover, knockdown of RIP1K attenuated astrocytic cell death and proliferation and promoted neuronal axonal generation in a neuron and astrocyte co-culture system. Both vascular endothelial growth factor D (VEGF-D) and its receptor VEGFR-3 were elevated in the reactive astrocytes; simultaneously, VEGF-D was increased in the medium of astrocytes exposed to OGD/Re. Knockdown of RIP1K down-regulated VEGF-D gene and protein levels in the reactive astrocytes. Treatment with 400 ng/ml recombinant VEGF-D induced the formation of glial scar; conversely, the inhibitor of VEGFR-3 suppressed OGD/Re-induced glial scar formation. RIP3K and MLKL may be involved in glial scar formation. Taken together, these results suggest that RIP1K participates in the formation of astrogliosis and glial scar via impairment of normal astrocyte responses and enhancing the astrocytic VEGF-D/VEGFR-3 signaling pathways. Inhibition of RIP1K promotes the brain functional recovery partially via suppressing the formation of astrogliosis and glial scar.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12975-021-00888-3DOI Listing
February 2021

CD90 affects the biological behavior and energy metabolism level of gastric cancer cells by targeting the PI3K/AKT/HIF-1α signaling pathway.

Oncol Lett 2021 Mar 7;21(3):191. Epub 2021 Jan 7.

NHC Key Laboratory of Carcinogenesis, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410013, P.R. China.

CD90, also known as Thy-1 cell surface antigen, is located on human chromosome 11q23.3, and encodes a glycosylphosphatidylinositol-linked cell surface glycoprotein. CD90 serves a key role in malignancy by regulating cell proliferation, metastasis and angiogenesis. Gastric cancer is one of the most common types of malignancy. Patients with advanced gastric cancer have a poor prognosis. CD90 plays a key role in the occurrence and progression of gastric cancer. However, the molecular mechanism of CD90 in gastric cancer is currently unclear. In order to identify the molecular mechanism by which CD90 affects the biological behavior and energy metabolism of gastric cancer cells, the present study used Cell Counting Kit-8 assays, lactate concentration determination and ATP content determination. The results demonstrated that CD90 promotes proliferation and inhibits senescence in gastric cancer cells. In addition, CD90 enhanced the invasion and migration abilities of AGS gastric cancer cells. Overexpression of CD90 resulted in the accumulation of intracellular lactic acid in AGS cells. CD90 upregulated lactate dehydrogenase levels and increased the NADPH/NADP ratio in AGS cells. CD90 overexpression decreased the ATP concentration in AGS cells. PI3K, PDK1, phosphorylated-AKT-Ser473, HIF-1α, MDM2 and SIRT1 levels were upregulated in CD90-overexpressing AGS cells, compared with AGS cells transfected with the empty vector. In contrast, PTEN, p53, SIRT2, SIRT3 and SIRT6 were downregulated. The results indicate that CD90 affects the biological behavior and levels of energy metabolism of gastric cancer cells by targeting the PI3K/AKT/HIF-1α signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/ol.2021.12451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816286PMC
March 2021

COVID-19: a comparison to the 1918 influenza and how we can defeat it.

Postgrad Med J 2021 05 9;97(1147):273-274. Epub 2021 Feb 9.

Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire, USA.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1136/postgradmedj-2020-139070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108277PMC
May 2021
-->