Publications by authors named "Liang Li"

3,935 Publications

  • Page 1 of 1

Pickering Emulsion Stabilized by Tea Seed Cake Protein Nanoparticles as Lutein Carrier.

Foods 2022 Jun 10;11(12). Epub 2022 Jun 10.

College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China.

To effectively deliver lutein, hydrothermally prepared tea seed cake protein nanoparticles (TSCPN) were used to fabricate Pickering emulsion, and the bioaccessibility of lutein encapsulated by Pickering emulsion and the conventional emulsion was evaluated in vitro. The results indicated that the average size and absolute value of zeta potential of TSCPN increased along with the increase in the protein concentration, and 2% protein concentration was adopted to prepare TSCPN. With the increase in the concentration of TSCPN, the size of Pickering emulsion decreased from 337.02 μm to 89.36 μm, and when the TSCPN concentration was greater than 0.6%, all emulsions exhibited good stability during the 14 days storage. Combined with the microstructure result, 1.2% TSCPN was used to stabilize Pickering emulsion. With the increase in ionic concentration (0-400 mM), the particle size of the emulsions increased while the absolute value of zeta potential decreased. TSCPN-based Pickering emulsion was superior to the conventional emulsion for both lutein encapsulation (96.6 ± 1.0% vs. 82.1 ± 1.4%) and bioaccessibility (56.0 ± 1.1% vs. 35.2 ± 1.2%). Thus, TSCPN-based Pickering emulsion in this study have the potential as an effective carrier for lutein.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/foods11121712DOI Listing
June 2022

Multi-channel GCN ensembled machine learning model for molecular aqueous solubility prediction on a clean dataset.

Mol Divers 2022 Jun 23. Epub 2022 Jun 23.

Laboratory of Molecular Design and Drug Discovery, School of Science, China Pharmaceutical University, 639 Longmian Avenue, Nanjing, 211198, China.

This study constructed a new aqueous solubility dataset and a solubility regression model which was ensembled by GCN and machine learning models. Aqueous solubility is a key physiochemical property of small molecules in drug discovery. In the past few decades, there have been many studies about solubility prediction. However, many of these studies have high root mean squared error (RMSE). Meanwhile, their dataset always contains salt compounds and solubility data obtained from different experimental conditions. In this paper, we constructed a clean dataset with 2609 compounds, which was small but contains only solubility records without salts at the same temperatures (25 °C). Here, we applied graph convolutional neural network (GCN) to construct an aqueous solubility prediction model. To enhance the performance of the model, the molecular MACCS key fingerprints and physiochemical descriptors were also combined with the GCN model to build a multi-channel model. Additionally, the authors also built two machine learning models (support vector regression and gradient boost decision tree) and assembled them to the GCN model to improve the root mean squared error (RMSE = 0.665). Finally, comparative experiments have shown that our framework achieved the best performance on ESOL dataset (RMSE = 0.56, RMSE = 0.44) and surpassed four established software on aqueous solubility prediction of new compounds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11030-022-10465-xDOI Listing
June 2022

HAO1-mediated oxalate metabolism promotes lung pre-metastatic niche formation by inducing neutrophil extracellular traps.

Oncogene 2022 Jun 23. Epub 2022 Jun 23.

Department of Pathology, Nanfang Hospital and Basic Medical College, Southern Medical University, Guangzhou, 510515, PR China.

Metabolic reprogramming has been shown to be involved in cancer-induced pre-metastatic niche (PMN) formation, but the underlying mechanisms have been insufficiently explored. Here, we showed that hydroxyacid oxidase 1 (HAO1), a rate-limiting enzyme of oxalate synthesis, was upregulated in the alveolar epithelial cells of mice bearing metastatic breast cancer cells at the pre-metastatic stage, leading to oxalate accumulation in lung tissue. Lung oxalate accumulation induced neutrophil extracellular trap (NET) formation by activating NADPH oxidase, which facilitated the formation of pre-metastatic niche. In addition, lung oxalate accumulation promoted the proliferation of metastatic cancer cells by activating the MAPK signaling pathway. Pharmacologic inhibition of HAO1 could effectively suppress the lung oxalate accumulation induced by primary cancer, consequently dampening lung metastasis of breast cancer. Breast cancer cells induced HAO1 expression and oxalate accumulation in alveolar epithelial cells by activating TLR3-IRF3 signaling. Collectively, these findings underscore the role of HAO1-mediated oxalate metabolism in cancer-induced lung PMN formation and metastasis. HAO1 could be an appealing therapeutic target for preventing lung metastasis of cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41388-022-02248-3DOI Listing
June 2022

FDA Approval Summary: Belantamab Mafodotin for Patients with Relapsed or Refractory Multiple Myeloma.

Clin Cancer Res 2022 Jun 23. Epub 2022 Jun 23.

United States Food and Drug Administration, Silver Spring, MD, United States.

On August 5, 2020, the Food and Drug Administration granted accelerated approval to belantamab mafodotin-blmf (BLENREP; GlaxoSmithKline) for the treatment of adult patients with relapsed or refractory multiple myeloma who have received at least 4 prior therapies including an anti-CD38 monoclonal antibody, a proteasome inhibitor, and an immunomodulatory agent. Substantial evidence of effectiveness was obtained from the phase 2, multicenter DREAMM-2 trial. Patients received belantamab mafodotin 2.5 mg/kg or 3.4 mg/kg intravenously once every 3 weeks until disease progression or unacceptable toxicity. The trial demonstrated an overall response rate of 31% in the 2.5 mg/kg cohort and 34% in the 3.4 mg/kg cohort. Keratopathy was the most frequent adverse event, occurring in 71% and 77% of patients, respectively. Other ocular toxicities included changes in visual acuity, blurred vision, and dry eye. The U.S. prescribing information for belantamab mafodotin includes a boxed warning for ocular toxicity, and belantamab mafodotin is available only through a restricted program under a Risk Evaluation and Mitigation Strategy. This article summarizes the data and the FDA review process supporting accelerated approval of belantamab mafodotin 2.5 mg/kg intravenously once every 3 weeks. This approval may be contingent upon verification and description of clinical benefit in confirmatory trial(s).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1158/1078-0432.CCR-22-0618DOI Listing
June 2022

Interferon-mediated repression of miR-324-5p potentiates necroptosis to facilitate antiviral defense.

EMBO Rep 2022 Jun 23:e54438. Epub 2022 Jun 23.

Cyrus Tang Hematology Center and Collaborative Innovation Center of Hematology, Jiangsu Institute of Hematology, Soochow University, Suzhou, China.

Mixed lineage kinase domain-like protein (MLKL) is the terminal effector of necroptosis, a form of regulated necrosis. Optimal activation of necroptosis, which eliminates infected cells, is critical for antiviral host defense. MicroRNAs (miRNAs) regulate the expression of genes involved in various biological and pathological processes. However, the roles of miRNAs in necroptosis-associated host defense remain largely unknown. We screened a library of miRNAs and identified miR-324-5p as the most effective suppressor of necroptosis. MiR-324-5p downregulates human MLKL expression by specifically targeting the 3'UTR in a seed region-independent manner. In response to interferons (IFNs), miR-324-5p is downregulated via the JAK/STAT signaling pathway, which removes the posttranscriptional suppression of MLKL mRNA and facilitates the activation of necroptosis. In influenza A virus (IAV)-infected human primary macrophages, IFNs are induced, leading to the downregulation of miR-324-5p. MiR-324-5p overexpression attenuates IAV-associated necroptosis and enhances viral replication, whereas deletion of miR-324-5p potentiates necroptosis and suppresses viral replication. Hence, miR-324-5p negatively regulates necroptosis by manipulating MLKL expression, and its downregulation by IFNs orchestrates optimal activation of necroptosis in host antiviral defense.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.15252/embr.202154438DOI Listing
June 2022

[Relationship between functional diversity and ecosystem multifunctionality of alpine meadow along an altitude gradient in Gannan, China].

Ying Yong Sheng Tai Xue Bao 2022 May;33(5):1291-1299

College of Geography and Environmental Science, Northwest Normal University, Lanzhou 730070, China.

Relationship between plant community functional diversity and ecosystem multifunctionality (EMF) was a new area of ecological research in recent years. Previous studies had mostly focused on the relationship between plant community functional diversity and individual ecosystem function, and lack of understanding of the EMF. In this study, six functional indices of aboveground biomass, soil organic carbon, soil total nitrogen, soil total phosphorus, soil available nitrogen and soil available phosphorus of Gannan alpine meadow were selected to analyze the relationship between plant community functional diversity and EMF on the altitude gradient of Gannan alpine mea-dow by using Bartlett sphericity test and multi-threshold method. The results showed that there was significant altitudinal difference in plant community composition, with species richness and plant coverage at 3500 m were significantly higher than those at other altitudes. Single and multi-functional diversity decreased with the increases of altitude, with significant difference among altitudes. Redundancy analysis showed that single and multi-functional richness, functional evenness and Rao's quadratic entropy were significantly positively correlated with soil temperature, soil water content and soil bulk density, and significantly negatively correlated with soil pH and soil conductivity. In a large threshold range (6%-89%), functional diversity had a significant positive effect on EMF. Based on correlation analysis, optimal regression model and random forest model, it was found that multi-functional richness index had a significant positive relationship with EMF, and that multi-functional richness was also the main driving factor of EMF. Overall, functional richness had the most significant impact on the EMF of alpine meadow in the Qinghai-Tibet Plateau.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13287/j.1001-9332.202205.003DOI Listing
May 2022

A potential fate decision landscape of the TWEAK/Fn14 axis on stem and progenitor cells: a systematic review.

Stem Cell Res Ther 2022 Jun 21;13(1):270. Epub 2022 Jun 21.

Department of Dermatology, The Second Affiliated Hospital of Xi'an Jiaotong University, 157 Xiwu Road, Xi'an, 710004, Shaanxi, China.

Stem and progenitor cells (SPCs) possess self-remodeling ability and differentiation potential and are responsible for the regeneration and development of organs and tissue systems. However, the precise mechanisms underlying the regulation of SPC biology remain unclear. Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) acts on miscellaneous cells via binding to fibroblast growth factor-inducible 14 (Fn14) and exerts pleiotropic functions in the regulation of divergent stem cell fates. TWEAK/Fn14 signaling can regulate the proliferation, differentiation, and migration of multiple SPCs as well as tumorigenesis in certain contexts. Although TWEAK's roles in modulating multiple SPCs are sparsely reported, the systemic effector functions of this multifaceted protein have not been fully elucidated. In this review, we summarized the fate decisions of TWEAK/Fn14 signaling on multiple stem cells and characterized its potential in stem cell therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13287-022-02930-zDOI Listing
June 2022

GSTP1 c.313A > G mutation is an independent risk factor for neutropenia hematotoxicity induced by anthracycline-/paclitaxel-based chemotherapy in breast cancer patients.

World J Surg Oncol 2022 Jun 22;20(1):212. Epub 2022 Jun 22.

Department of Medical Oncology, Meizhou People's Hospital (Huangtang Hospital), Meizhou Academy of Medical Sciences, Meizhou, People's Republic of China.

Background: The link between glutathione S-transferase P1 (GSTP1) c.313A > G polymorphism and chemotherapy-related adverse events remains controversial. The goal of this study was to assess how this variant affected the toxicity of anthracycline-/paclitaxel-based chemotherapy in patients with breast cancer.

Methods: This study retrospectively investigated pharmacogenetic associations of GSTP1 c.313A > G with chemotherapy-related adverse events in 142 breast cancer patients who received anthracycline and/or paclitaxel chemotherapy.

Results: There were 61 (43.0%), 81 (57.0%), 43 (30.3%), and 99 (69.7%) patients in the T0-T2, T3-T4, N0-N1, and N2-N3 stages, respectively. There were 108 (76.1%) patients in clinical stages I-III and 34 (23.9%) patients in clinical stage IV. The numbers of patients with luminal A, luminal B, HER2 + , and triple-negative breast cancer (TNBC) were 10 (7.0%), 77 (54.2%), 33 (23.2%), and 22 (15.5%), respectively. The numbers of patients who carried GSTP1 c.313A > G A/A, A/G, and G/G genotypes were 94 (66.2%), 45 (31.7%), and 3 (2.1%), respectively. There were no statistically significant differences in the proportion of certain toxicities in patients with A/G, G/G, and A/G + G/G genotypes, except for neutropenia, in which the proportion of patients with A/G + G/G (χ = 6.586, P = 0.035) genotypes was significantly higher than that with the AA genotype. The logistic regression analysis indicated that GSTP1 c.313A > G mutation (A/G + G/G vs. A/A genotype) (adjusted OR 4.273, 95% CI 1.141-16.000, P = 0.031) was an independent variable associated with neutropenia.

Conclusions: The findings of this study indicate that the GSTP1 c.313A > G mutation is an independent risk factor for neutropenia hematotoxicity in breast cancer patients induced by anthracycline-/paclitaxel-based chemotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12957-022-02679-yDOI Listing
June 2022

Progress on the formation mechanism of sexual dimorphism plumage color in birds.

Yi Chuan 2022 Jun;44(6):491-500

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

Sexually dimorphic plumage coloration is widespread in birds in which the male plumage is brighter than the female. This phenomenon is related to the environmental constraints on sexual selection or intraspecific competition between males and females in birds. The physiological factors and genetic regulation mechanism affecting the color of sexual dimorphism plumages in birds have always attracted significant attention in research. Understanding the diversity of sexually dimorphic traits provides insights into the mating strategies of the sexes and their behavior, ecology, and evolution. Interestingly, the ASIP, MC1R, TYRP1, and BCO2 genes have been identified to play a potential role in the coloration of melanin and carotenoids in bird sexual dimorphism plumages, either by controlling the rate and type of melanin or carotene synthesis or degradation by exerting an effect on the pigment biosynthetic pathway. In this review, we systematically summarize the biological significance, the direct causes (chemical and physical color), and the influence of sex hormones in sexually dimorphic plumage coloration. We also investigate the molecular mechanism underlying the roles of some genes on sexual dimorphism coloration, thereby providing a reference for in-depth understanding on the formation mechanism(s) of sexual dimorphic coloration in birds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.16288/j.yczz.22-004DOI Listing
June 2022

Interference with connective tissue growth factor attenuated fibroblast-to-myofibroblast transition and pulmonary fibrosis.

Ann Transl Med 2022 May;10(10):566

Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.

Background: The aberrant activation and phenotype shift of resident fibroblasts in lung tissues via fibroblast-to-myofibroblast transition (FMT) is considered a pivotal step in pulmonary fibrogenesis, resulting in excessive extracellular matrix (ECM) production and deposition. However, the molecular mechanisms regulating FMT and lung fibrosis are still unclear. Connective tissue growth factor (CTGF) has been reported to be both an ECM protein and a versatile signaling molecule that is involved in multiple pathophysiological contexts, especially fibrosis. The relationship between CTGF, FMT, and lung fibrosis has not yet been well defined.

Methods: In this study, a pulmonary fibrosis (PF) rat model and FMT cell model induced by paraquat (PQ) were established to explore the relevant regulatory mechanisms and .

Results: The results showed that the CTGF was highly activated and was a mediator of canonical Wnt signaling during FMT and PF. The inhibition of the CTGF by small-interfering ribonucleic acid decreased the expression of FMT markers, including α-smooth muscle actin, vimentin, and collagen I, inhibited the activated Wnt signaling pathway, and ameliorated lung fibrosis.

Conclusions: Our findings showed that CTGF was the key effector of the FMT and fibrotic changes, and emphasized the therapeutic potential of the inhibitor or monoclonal antibody against CTGF for PF.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-22-1397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201195PMC
May 2022

Real-Time Visual Sensing of Heat or Mass Transfer Processes for Microfluids via Tamm Plasmon Polaritons.

Authors:
Haoyue Hao Liang Li

ACS Omega 2022 Jun 31;7(23):20376-20382. Epub 2022 May 31.

School of Physics and Optoelectronic Engineering, Shandong University of Technology, Zibo 255000, China.

Heat or mass transfer processes of microfluids are very important in bioscience, environmental engineering, and food science, which are still hard to detect in real time. To overcome this difficulty, we try to use Tamm plasmon polaritons to enhance the interaction of light with microfluids. The main structure of the proposed configuration is Ag-photonic crystal (PhC) cavity, which can generate strong photonic localization by exciting Tamm plasmon polaritons. The results show that the enhancement of light intensity reaches ∼90 times in the cavity and the reflectance spectrum of the proposed structure exists in a narrow valley near 632.8 nm. This illustrates the generation of Tamm plasmon polaritons in the proposed structure. By injecting the microfluids into the cavity, the heat and mass transfer processes of the microfluids will have considerable influence on the reflectance of the proposed structure. Simulation results show that the concentration or temperature distributions of the microfluids can be effectively detected by analyzing the brightness of the imaging pictures, which is real-time and visible. Meanwhile, the sensitivity of the proposed configuration can be tuned by setting proper base parameters. This proposed configuration will have great potential in the study of microfluids, especially for the dynamic processes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.2c02481DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201882PMC
June 2022

Complete chloroplast genome sequence of (Myrtaceae) and phylogenetic analysis.

Mitochondrial DNA B Resour 2022 10;7(6):977-979. Epub 2022 Jun 10.

Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, China.

(Blume) Merr. et Perry, 1938, commonly known as wax apple, is a Myrtaceae species that is known for its unique fruit shape, flavorful and colorful fruits, medicinal value and increasing economic relevance. In this study, we reported the complete chloroplast genome sequence of . The complete genome is 159,109 bp in length with a quadripartite structure containing two single copy regions, a Large Single Copy region (LSC, 88,155 bp) and a Small Single Copy region (SSC, 18,796 bp) separated by Inverted Repeat regions (IRs, 26,079 bp). The GC content was 37.0%. It encoded 126 genes, including 81 protein-coding genes, 37 transfer RNA genes, and 8 ribosomal RNA genes. The phylogenetic relationships of 20 species inferred that all species formed a single cluster belonging to Syzygieae tribe. Our results offer insights into the evolutionary relationship of within Myrtaceae, indicating a closer relationship between and i.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/23802359.2022.2080022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196770PMC
June 2022

IGST Combined with Conventional Drug Therapy and TCM Therapy for Treatment of Bilateral Vestibular Hypofunction.

Evid Based Complement Alternat Med 2022 7;2022:5689454. Epub 2022 Jun 7.

Department of Otolaryngology-Head and Neck Surgery, No. 2 Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.

Objective: To investigate the effects of intensive gaze stability training (IGST) combined with conventional drug therapy and traditional Chinese medicine (TCM) therapy on hearing recovery and quality of life (QOL) in patients with bilateral vestibular dysfunction.

Materials And Methods: A total of 120 patients with bilateral vestibular dysfunction admitted to our hospital from January 2019 to January 2020 were recruited and assigned to group A ( = 60) and group B ( = 60) based on the order of admission. Group B received conventional medication and TCM therapy, and group A received IGST plus conventional medication and TCM therapy. The serum biochemical indexes, blood rheology indexes, symptoms disappearance time, hearing recovery time, pure-tone hearing threshold, vestibular symptom index (VSI), and QOL of the two groups were compared.

Results: The serum biochemical indexes and blood rheology indexes in group A were lower than those in group B after treatment ( < 0.001). Patients in group A had a shorter time lapse before symptoms disappearance and hearing recovery than those in group B ( < 0.001). Group A had lower results of the pure-tone hearing threshold ( < 0.001) and of VSI ( < 0.05) than group B. Patients in group A had higher QOL scores than those in group B after treatment ( < 0.001).

Conclusion: IGST plus conventional drug therapy effectively improves the serum biochemical indexes and blood rheological indexes of patients with bilateral vestibular hypofunction, accelerates their hearing recovery process, and alleviates their clinical symptoms, thereby improving their QOL, which shows good potential in clinical application.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/5689454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9197658PMC
June 2022

SARS-CoV-2 productively infects human brain microvascular endothelial cells.

J Neuroinflammation 2022 Jun 15;19(1):149. Epub 2022 Jun 15.

State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.

Background: The emergence of the novel, pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global health emergency. SARS-CoV-2 is highly contagious and has a high mortality rate in severe patients. However, there is very limited information on the effect of SARS-CoV-2 infection on the integrity of the blood-brain barrier (BBB).

Methods: RNA-sequencing profiling was performed to analyze the transcriptomic changes in human brain microvascular endothelial cells (hBMECs) after SARS-CoV-2 infection. Bioinformatic tools were used for differential analysis. Immunofluorescence, real-time quantitative PCR, and Western blotting analysis were used to explore biological phenotypes.

Results: A total of 927 differentially expressed genes were identified, 610 of which were significantly upregulated while the remaining 317 were downregulated. We verified the significant induction of cytokines, chemokines, and adhesion molecules in hBMECs by SARS-CoV-2, suggesting an activation of the vascular endothelium in brain. Moreover, we demonstrated that SARS-CoV-2 infection could increase the BBB permeability, by downregulating as well as remodeling the intercellular tight junction proteins.

Conclusions: Our findings demonstrated that SARS-CoV-2 infection can cause BBB dysfunction, providing novel insights into the understanding of SARS-CoV-2 neuropathogenesis. Moreover, this finding shall constitute a new approach for future prevention and treatment of SARS-CoV-2-induced CNS infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12974-022-02514-xDOI Listing
June 2022

Antibiotic-loaded Bone Cement Combined with Vacuum-assisted Closure Facilitating Wound Healing in Wagner 3-4 Diabetic Foot Ulcers.

Int J Low Extrem Wounds 2022 Jun 15:15347346221109045. Epub 2022 Jun 15.

Department of Vascular Surgery, 527527The Affiliated Qingdao Central Hospital of Qingdao University, Qingdao, Shandong, PR China.

This study explored the clinical effectiveness of antibiotic-loaded bone cement (ALBC) combined with vacuum-assisted closure (VAC) on the treatment of Wagner 3-4 diabetic foot ulcers (DFUs). This is a retrospective study, including 32 patients with Wagner 3-4 DFUs who had undergone treatment between August 2019 and June 2021. Patient age, sex, Hemoglobin A1c (HbA1c), body mass index (BMI), ankle brachial index (ABI), white blood cells (WBC), C-reactive protein (CRP) levels, comorbidities and wound closure methods were recorded. Patients were divided into the study group and control group according to the treatment modality. Patients in the study group received the combination treatment of ALBC and VAC, while patients in the control group received single VAC treatment. Clinical endpoints were assessed and compared between the two groups, including wound complete healing time and complications after operation. All patients were followed-up 6 months postoperation. Results showed that the mean healing time of the study group (44.20 ± 16.72 days) was shorter than that of the control group (64.00 ± 29.85 days) ( < .05). The infection rate of the study group on the 10th day postoperation was lower than that of the control group (6.67%, 47.06%,  < .05). And there were no significant statistical differences between the two groups in terms of bleeding and necrosis complications postoperation ( = .603,  = .603). Based on the findings, we conclude that the application of ALBC combined with VAC can facilitate wound healing in Wagner 3-4 DFUs patients. It can help to control wound infections and shorten wound healing time.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/15347346221109045DOI Listing
June 2022

A Single-Dot Perovskite Spectrometer.

Adv Mater 2022 Jun 15:e2200221. Epub 2022 Jun 15.

School of Physical Science and Technology, Jiangsu Key Laboratory of Thin Films, Center for Energy Conversion Materials & Physics (CECMP), Soochow University, Suzhou, 215006, P. R. China.

There are significant applications for miniature on-chip spectrometers in many fields. However, at present, on-chip spectrometers have to utilize an integrated strategy to achieve spectral analysis, which undoubtedly squanders the photosensitive area and adds pressure to the miniaturization of the spectrometer. Here, we demonstrate a unique spectrometer design that adopts a single detection point with in situ modulation realized by the photo-gain control at various bias voltages. With micrometre-level footprints, this single-dot spectrometer processes a resolution of about 5 nm and a response time down to about 197 μs. This is the first in situ perovskite modulation strategy that breaks the footprint-resolution restriction of spectrum analysis and demonstrates a new design direction for functional perovskite devices. This article is protected by copyright. All rights reserved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/adma.202200221DOI Listing
June 2022

Optimization of germination and ultrasonic-assisted extraction for the enhancement of γ-aminobutyric acid in pumpkin seed.

Food Sci Nutr 2022 Jun 21;10(6):2101-2110. Epub 2022 Mar 21.

Beijing Key Laboratory of Flavor Chemistry Beijing Technology and Business University (BTBU) Beijing China.

Germination and ultrasonic-assisted extraction (UAE) are economical and effective methods to enhance bioactive compounds in plant seeds. We optimized the germination parameters and UAE parameters by using response surface methodology to maximize the recovery of γaminobutyric acid (GABA) in pumpkin seeds. The optimal germination conditions were as follows: soaking the seeds at 28°C for 6 h with 0.2% CaCl, 3.8 mg/ml monosodium glutamate, and 4.0 mg/ml vitamin B, then germination at 30°C for 61.6 h. The optimal conditions for UAE were as follows: 1:75 (w/v) material-to-solvent ratio, 220 W ultrasonic power, and ultrasonic treatment at 50°C for 14.4 min, which afforded an extraction yield of 2679 ± 10 mg/100 g. Moreover, the GABA-enhanced extract showed the potential of hypolipidemic effect in type 2 diabetes rats. These results confirmed that a combination of germination and UAE increased the GABA yield from pumpkin seeds and provided a basis for GABA-enhanced production to improve lifestyle-associated diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/fsn3.2826DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9179130PMC
June 2022

Effect of the mineral-microbial complexes on the quality, soil nutrients, and microbial community of tailing substrates for growing potted Rorippa.

Microbiol Res 2022 Jun 1;262:127084. Epub 2022 Jun 1.

Key Laboratory of Ministry of Education on Safe Mining of Deep Metal Mines, Northeastern University, Shenyang 110819, PR China. Electronic address:

With China's industrialization and a rapidly developing coal industry, tailings have become one of the most widely distributed solid wastes, responsible for degrading available land and damaging the surrounding ecological environment. This study investigated the effect of adding mineral-microbial complexes to tailing substrates for the improvement of plant growth and substrate microbial community. The results revealed that compared with other treatments, the growth of Rorippa was considerably better after the addition of mineral-microbial complexes to the substrate, indicating that the mineral-microbial complexes promoted plant growth. After the addition of mineral-microbial complexes, the fertility indicators of the substrate showed a substantial improvement, in addition to the pH and organic matter (OM). The addition of fertilizers to the substrate plays a key role in plant growth, whereas the addition of microbial supplements to the substrate alone has little effect on plant growth. The results of high-throughput sequencing showed that the main microbial communities present in the substrate were Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes, and Nitrospirae. The results of the microbial community α-diversity analysis showed that the addition of the mineral-microbial complexes improved the abundance and diversity of the substrate microbial community. Results of the microbial community β-diversity analysis indicated that the experimental group showed a higher correlation with the microbial community relative to the background group. Network analysis revealed similar correlations between microbial communities and environmental factors, and total phosphorous (TP)-pH-available potassium (AK)-available nitrogen (AN) and TP-electronic conductivity (EC)-AK-AN were the main drivers of microbial communities in the background and experimental groups, respectively. The findings of this study provide a theoretical basis for the resource utilization of tailings and vegetation restoration using tailings.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.micres.2022.127084DOI Listing
June 2022

Subcritical Water Enhanced with Deep Eutectic Solvent for Extracting Polysaccharides from and Their Antioxidant Activities.

Molecules 2022 Jun 4;27(11). Epub 2022 Jun 4.

College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China.

In the present study, subcritical water extraction (SWE) assisted with deep eutectic solvent (DES) is used to extract polysaccharides (LEP). In addition, the antioxidant activity of the polysaccharide samples was also investigated. Based on a single factor test and response surface test, the optimal extraction factors were a liquid-solid solvent of 40:1 mL/g, extraction temperature of 147.23 °C, water content of 39.76% and extraction time of 17.58 min. Under these extraction conditions, the yield of LEP was 6.26 ± 0.08%. Compared with the SWE and hot water extraction (HWE), it improved by 19.24% and 17.01%, respectively. In addition, the results of monosaccharide composition, molecular weight, FT-IR, UV and SEM confirmed that the extracts had the features of polysaccharides. Interestingly, the polysaccharides obtained with the SWE assisted with the DES procedure showed a higher DPPH scavenging activity, hydroxyl radical scavenging activity and hydrogen peroxide scavenging activity, which indicated that the polysaccharides with this method had a stronger antioxidant activity. These findings demonstrated that the SWE-assisted DES is a strong method to obtain polysaccharides from for food, biopharmaceutical and other industrial production.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules27113612DOI Listing
June 2022

Whey Protein Isolate Nanofibers Prepared by Subcritical Water Stabilized High Internal Phase Pickering Emulsion to Deliver Curcumin.

Foods 2022 May 31;11(11). Epub 2022 May 31.

College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China.

This study aimed to design a Pickering emulsion (PE) stabilized by whey protein isolate nanofibers (WPINs) prepared with subcritical water (SW) to encapsulate and prevent curcumin (Cur) degradation. Cur-loaded WPINs-SW stabilized PE (WPINs-SW-PE) and hydrothermally prepared WPINs stabilized PE (WPINs-H-PE) were characterized using the particle size, zeta potential, Congo Red, CD, and TEM. The results indicated that WPINs-SW-PE and WPINs-H-PE showed regular spherical shapes with average lengths of 26.88 ± 1.11 μm and 175.99 ± 2.31 μm, and zeta potential values were -38.00 ± 1.00 mV and -34.60 ± 2.03 mV, respectively. The encapsulation efficiencies of WPINs-SW-PE and WPINs-H-PE for Cur were 96.72 ± 1.05% and 94.07 ± 2.35%. The bio-accessibility of Cur of WPINs-SW-PE and WPINs-H-PE were 57.52 ± 1.24% and 21.94 ± 2.09%. In addition, WPINs-SW-PE had a better loading effect and antioxidant activities compared with WPINs-H-PE. SW could be a potential processing method to prepare a PE, laying the foundation for the subsequent production of functional foods.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/foods11111625DOI Listing
May 2022

Morphology and molecular characterization of Acanthogyrus (Acanthosentis) bilaspurensis Chowhan, Gupta & Khera, 1987 (Acanthocephala: Gyracanthocephala: Quadrigyridae) from the common carp Cyprinus carpio Linnaeus (Cypriniformes: Cyprinidae) in Pakistan.

Parasitol Int 2022 Jun 6;90:102608. Epub 2022 Jun 6.

Key Laboratory of Molecular Cell Biology, Ministry of Education, Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, College of Life Sciences, Hebei Normal University, 050024 Shijiazhuang, Hebei Province, PR China. Electronic address:

Acanthogyrus (Acanthosentis) bilaspurensis Chowhan, Gupta & Khera, 1987 is a poorly known acanthocephalan species reported from the reba carp Cirrhinus reba (Hamilton) (Cypriniformes: Cyprinidae) in India. In the present study, the detailed morphology of A. (Acanthosentis) bilaspurensis were studied using light microscopy and, for the first time, scanning electron microscopy (SEM), based on newly collected specimens from the common carp Cyprinus carpio Linnaeus (Cypriniformes: Cyprinidae) in Pakistan. The SEM observations revealed the outer shell of eggs with numerous remarkable protuberances and the gonopore of female located at a discoid protrusion surrounded by some very small spines. This is the first time that A. (Acanthosentis) bilaspurensis was reported in Pakistan, and the common carp represents a new host for this species. In addition, the molecular characterization of the 18S and 28S rDNA of A. (Acanthosentis) bilaspurensis is provided for the first time. There was no intraspecific genetic variation detected in the 18S and 28S regions between different individuals of A. (Acanthosentis) bilaspurensis, but high level of interspecific nucleotide divergence was found in the 18S (4.15-16.7%) and 28S regions (3.60-15.4%). Phylogenetic results based on the 18S, 28S and 18S + 28S sequence data, respectively, all revealed the Quadrigyridae, Pallisentinae and the genus Neoechinorhynchus are not monophyletic groups, and the genera Acanthogyrus and Pallisentis have far relationship. The systematic status of the genus Acanthogyrus and some species of Neoechinorhynchus need further clarify based on different mtDNA and nuclear DNA data including broader representatives of the class Eoacanthocephala.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.parint.2022.102608DOI Listing
June 2022

Effects of cage versus floor rearing system on goose intestinal histomorphology and cecal microbial composition.

Poult Sci 2022 Jul 26;101(7):101931. Epub 2022 Apr 26.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address:

Due to the demand for modern goose production and the pressure of environmental protection, the rearing systems of geese are changing from traditional waterside rearing to intensive rearing systems such as floor rearing (FR) and cage rearing (CR) systems. However, little is known about the effects of different rearing systems on goose intestinal functions and cecal microbial composition. Therefore, this study aimed to compare intestinal histomorphology and cecal microbial composition differences in geese reared under CR and FR at 270 d of age. Histomorphological analysis showed that the ileal villus height (VH) to crypt depth (CD) ratio was significantly greater in CR than in FR (P < 0.001). Taxonomic analysis showed that the dominant bacteria of cecal microorganisms in both rearing systems were roughly similar, with Bacteroidota, Firmicutes, Fusobacteriota, and Proteobacteria being the dominant phyla while Bacteroides, Fusobacterium, and uncultured_bacterium_o_Bacteroidales being the dominant genera. Differentially abundant taxa between CR and FR were also identified using Linear Discriminant Analysis Effect Size (LEfSe) analysis (P < 0.05, LDA score > 3.5). Megamonas and Anaerobiospirillum were significantly enriched in the CR group at the genus level, while uncultured_bacterium_f_Rikenellaceae and Sutterella were significantly enriched in the FR group. Notably, we found that the relative abundance of uncultured_bacterium_f_Rikenellaceae was significantly negatively correlated with the ileal VH and VH/CD (P < 0.05). The relative abundance of Megamonas and Anaerobiospirillum were significantly negatively correlated with abdominal fat weight and relative abdominal fat weight (P < 0.01), whereas that of Sutterella was significantly positively correlated with abdominal fat weight and relative abdominal fat weight (P < 0.01). Furthermore, PICRUSt2 analysis indicated that the lipid metabolism pathways of cecal microorganisms were lower enriched in CR than in FR. In conclusion, compared with FR, the CR significantly changed goose ileal histomorphological characteristics and cecal microbial composition, thereby affecting goose physiological functions and production performance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.psj.2022.101931DOI Listing
July 2022

Evaluation of Serum Ferritin, Procalcitonin, and C-Reactive Protein for the Prediction of Severity and Mortality in Hemorrhagic Fever With Renal Syndrome.

Front Microbiol 2022 23;13:865233. Epub 2022 May 23.

Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China.

This study aimed to analyze the clinical significance of serum ferritin, procalcitonin (PCT), and C-reactive protein (CRP) in patients with hemorrhagic fever with renal syndrome (HFRS). The demographical, clinical, and laboratory data of 373 patients with HFRS in northeastern China were retrospectively analyzed. The levels of serum ferritin and PCT in severe patients ( = 108) were significantly higher than those in mild patients ( = 265, < 0.001) and associated with HFRS severity. The area under the receiver operating characteristic curve (AUC) values of serum ferritin and PCT for predicting the severity of HFRS were 0.732 (95% CI 0.678-0.786, < 0.001) and 0.824 (95% CI 0.773-0.875, < 0.001), respectively, showing sensitivity and specificity of 0.75 and 0.88 for serum ferritin, and 0.76 and 0.60 for PCT. The CRP level in HFRS with bacterial co-infection ( = 115) was higher than that without bacterial co-infection ( = 258, < 0.001). The AUC value of CRP for predicting bacterial co-infection was 0.588 (95% CI 0.525-0.652, < 0.001), showing sensitivity and specificity of 0.43 and 0.76, respectively. The serum ferritin level in non-survivors ( = 14) was significantly higher than in survivors ( = 359, < 0.001). The AUC value of serum ferritin for predicting mortality was 0.853 (95% CI 0.774-0.933, < 0.001), showing sensitivity and specificity of 0.933 and 0.739. Serum ferritin and PCT have a robust association with HFRS severity and mortality, which may be promising predictors, and CRP is an effective biomarker to assess bacterial co-infection in HFRS.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2022.865233DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9169039PMC
May 2022

A 33-color panel of phenotypic analysis of murine organ specific immune cells.

J Immunol Methods 2022 Jun 5;507:113294. Epub 2022 Jun 5.

Medical Research Center, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China. Electronic address:

This 33-color panel delineates immune phenotypes of the major lymphocyte subsets in murine tissues. The panel identifies surface markers for cell activation, differentiation, and exhaustion, and chemokine receptors, in CD4 T cells, CD8 T cells, γδ T cells, NK cells, NKT cells and B cells in spleen and liver. This panel was designed to include only surface markers to avoid the need for fixation and permeabilization steps. Cells were analyzed on a full spectrum flow cytometer, 5-Laser Aurora system (Cytek Biosciences). This panel enables in-depth immunophenotyping of murine lymphocytes in immune and non-immune tissues of mice. It could be a tool for systematic analysis of immune cell response to infectious diseases and immune disorders.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jim.2022.113294DOI Listing
June 2022

Multimodality imaging of cor triatriatum dexter complicated with hypertrophic cardiomyopathy of restrictive phenotype.

Authors:
Li Liang Min-Jie Lu

Radiol Case Rep 2022 Aug 28;17(8):2598-2602. Epub 2022 May 28.

Department of Magnetic Resonance Imaging, Fuwai Hospital and National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beilishi Road No. 167, Xicheng District, Beijing 100037, China.

Cor triatriatum dexter (CTD) is an extremely rare congenital cardiac malformation in which a membrane divides the right atrium into 2 chambers. Hypertrophic cardiomyopathy (HCM) with restrictive phenotype is also a rare cardiomyopathy. We report a case with an 18-year history of chest discomfort, fatigue and syncope following intense physical activity was finally diagnosed with CTD complicated with HCM, and the HCM is a special type, restrictive phenotype. Multimodal imaging was used to diagnose this complex disease and analyzed the main cause of her heart failure, which provided accurate evidence for clinical treatment and prognosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.radcr.2022.03.087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157214PMC
August 2022

Multi-slice spiral computed tomography in diagnosing unstable pelvic fractures in elderly and effect of less invasive stabilization.

World J Clin Cases 2022 May;10(14):4470-4479

Department of Traumatic Orthopedics Sports Medicine, Yan'an Hospital of Traditional Chinese Medicine, Yan'an 716000, Shaanxi Province, China.

Background: Older people are more likely to experience pelvic fractures than younger people. Multi-slice spiral computed tomography (CT) uses three-dimensional (3D) reconstruction technology to generate 3D images that can clearly demonstrate the 3D space of fractures and detect fractures at a higher rate.

Aim: To investigate the clinical value of multi-slice spiral CT 3D reconstruction in the diagnosis of unstable pelvic fractures in the elderly as well as the effect of less invasive stabilization.

Methods: A total of 86 patients with unstable pelvic fractures treated between March 2016 and March 2019 underwent femoral supracondylar bone traction before surgery. Pelvic radiography and multi-row spiral CT were performed successively once the patient's vital signs and hemodynamic indices were stable. Secondary processing of the original data was performed to obtain 3D reconstruction images and determine the vertical displacement of the pelvis. After basic or complete reduction, minimally invasive internal fixation using hollow lag screws was performed. The detection rates of fracture location and classification by X-ray and CT reconstruction were compared. Patients were divided into two groups according to the presence or absence of preoperative 3D reconstruction to compare postoperative reduction, wound healing time, fracture healing time, hospitalization time, visual analog scale (VAS) score, poor internal fixation, and functional recovery.

Results: The diagnostic coincidence rates of X-rays for pubic symphysis, ilium wing, sacroiliac periarticular, and sacral fractures were lower than those of CT reconstruction. The coincidence rate of CT reconstruction in the clinical classification of pelvic fractures was 100%, whereas 11 cases were misdiagnosed by X-ray; the total coincidence rate was 87.21%. The total excellent and good rates of postoperative reduction were significantly higher in the study group than in the control group ( < 0.05). The wound healing, fracture healing, and hospitalization times were significantly shorter in the study group than in the control group ( < 0.05). The VAS scores decreased in both groups postoperatively and were lower in the study group than in the control group ( < 0.05). The total incidence of poor postoperative internal fixation was significantly lower in the study group than in the control group ( < 0.05). The overall rate of postoperative functional recovery was significantly higher in the study group than in the control group ( < 0.05).

Conclusion: Multi-slice spiral CT has high guiding significance for the diagnosis, classification, and treatment of unstable pelvic fractures in the elderly. Preoperative 3D reconstruction can effectively shorten the operation time and promote fracture healing, while minimally invasive internal fixation can effectively reduce pain and promote functional recovery of fracture sites, making it worthy of clinical application.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12998/wjcc.v10.i14.4470DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125281PMC
May 2022

Magnitude and breadth of antibody cross-reactivity induced by recombinant influenza hemagglutinin trimer vaccine is enhanced by combination adjuvants.

Sci Rep 2022 Jun 2;12(1):9198. Epub 2022 Jun 2.

Vaccine Research and Development Center, Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA, 92697, USA.

The effects of adjuvants for increasing the immunogenicity of influenza vaccines are well known. However, the effect of adjuvants on increasing the breadth of cross-reactivity is less well understood. In this study we have performed a systematic screen of different toll-like receptor (TLR) agonists, with and without a squalene-in-water emulsion on the immunogenicity of a recombinant trimerized hemagglutinin (HA) vaccine in mice after single-dose administration. Antibody (Ab) cross-reactivity for other variants within and outside the immunizing subtype (homosubtypic and heterosubtypic cross-reactivity, respectively) was assessed using a protein microarray approach. Most adjuvants induced broad IgG profiles, although the response to a combination of CpG, MPLA and AddaVax (termed 'IVAX-1') appeared more quickly and reached a greater magnitude than the other formulations tested. Antigen-specific plasma cell labeling experiments show the components of IVAX-1 are synergistic. This adjuvant preferentially stimulates CD4 T cells to produce Th1>Th2 type (IgG2c>IgG1) antibodies and cytokine responses. Moreover, IVAX-1 induces identical homo- and heterosubtypic IgG and IgA cross-reactivity profiles when administered intranasally. Consistent with these observations, a single-cell transcriptomics analysis demonstrated significant increases in expression of IgG1, IgG2b and IgG2c genes of B cells in H5/IVAX-1 immunized mice relative to naïve mice, as well as significant increases in expression of the IFNγ gene of both CD4 and CD8 T cells. These data support the use of adjuvants for enhancing the breath and durability of antibody responses of influenza virus vaccines.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-022-12727-yDOI Listing
June 2022

Identification of Two Genetic Loci Associated with Leukopenia after Chemotherapy in Breast Cancer Patients.

Clin Cancer Res 2022 Jun 2. Epub 2022 Jun 2.

Erlangen University Hospital, Comprehensive Cancer Center Erlangen-EMN, Friedrich Alexander University of Erlangen-Nuremberg, Erlangen, Germany.

Purpose: To identify molecular predictors of grade 3/4 neutropenic or leukopenic events (NLEs) after chemotherapy using a genome-wide association study (GWAS).

Patients And Methods: A GWAS was performed on patients in the phase III chemotherapy study SUCCESS-A (n = 3322). Genotyping was done using the Illumina HumanOmniExpress-12v1 array. Findings were functionally validated with cell culture models and the genotypes and gene expression of possible causative genes were correlated with clinical treatment response and prognostic outcomes.

Results: One locus on chromosome 16 (rs4784750; NLRC5; P = 1.56E-8) and another locus on chromosome 13 (rs16972207; TNFSF13B; P = 3.42E-8) were identified at a genome-wide significance level. Functional validation revealed that expression of these two genes is altered by genotype-dependent and chemotherapy-dependent activity of two transcription factors. Genotypes also showed an association with disease-free survival in patients with an NLE.

Conclusions: Two loci in NLRC5 and TNFSF13B are associated with NLEs. The involvement of the major histocompatibility complex I regulator NLRC5 implies the possible involvement of immuno-oncological pathways.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1158/1078-0432.CCR-20-4774DOI Listing
June 2022

The influence of protease hydrolysis of lactic acid bacteria on the fermentation induced soybean protein gel: Protein molecule, peptides and amino acids.

Authors:
Yiming Ren Liang Li

Food Res Int 2022 Jun 22;156:111284. Epub 2022 Apr 22.

College of Food Science, Northeast Agricultural University, Harbin 150030, China. Electronic address:

The purpose explored the influence of protease hydrolysis of lactic acid bacteria and its hydrolysates on the fermentation induced soybean protein gel. The changes of protein molecule, peptides and amino acids after hydrolysis were analyzed. With the extension of fermentation time, the molecules (≤30 kDa) of L. paracasei-gel and L. plantarum-gel increased to 68.8% and 63%, the surface hydrophobicity of protein was increased, internal sulfhydryl groups and soluble proteins were reduced. These results indicated physicochemical properties of protein molecules were changed, small molecular proteins produced by hydrolysis were aggregated through intermolecular forces to promote gel formation. Peptide profiles and amino acid profiles showed that the peptides (≤10AA) of L. casei-gel increased by 3.32%, the peptides derived from 7S protein increased by more than 5%, the hydrophobic peptides and hydrophobic amino acids increased, these enzymatic hydrolysates may form insoluble aggregate through intermolecular forces to promote gel formation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.foodres.2022.111284DOI Listing
June 2022

Efficacy and safety of a basal insulin + 2-3 oral antihyperglycaemic drugs regimen versus a twice-daily premixed insulin + metformin regimen after short-term intensive insulin therapy in individuals with type 2 diabetes: the multicentre, open-label, randomised controlled BEYOND-V trial.

Diabetes Obes Metab 2022 Jun 1. Epub 2022 Jun 1.

Department of Endocrinology, Beijing Hospital, National Center of Gerontology, Beijing, China.

Aim: To compare the efficacy and safety of basal insulin glargine 100 units/mL (Gla) + 2-3 oral antihyperglycaemic drugs (OADs) with twice-daily premixed insulin aspart 70/30 (Asp30) + metformin (MET) after short-term intensive insulin therapy in adults with type 2 diabetes in China.

Materials And Methods: This open-label trial enrolled insulin-naïve adults with type 2 diabetes and an HbA1c 7.5-11.0% (58-97 mmol/mol) despite 2-3 OADs treatment. All participants stopped previous OADs except MET, then received short-term intensive insulin therapy during the run-in period, who with fasting plasma glucose (FPG) <7.0 mmol/L and 2-hour postprandial glucose <10.0 mmol/L were randomized to Gla + MET + DPP4i or twice-daily Asp30 + MET. If HbA1c was >7.0% (>53 mmol/mol) at week 12, participants in the insulin glargine group were added repaglinide or acarbose, at the physician's discretion, and participants in the premix insulin group continued to titrate insulin dose, The change in HbA1c from baseline to week 24 was assessed in the per protocol (PP) population (primary endpoint).

Results: There were 384 enrollees (192 each to Gla and Asp30); 367 were included in the PP analysis. The threshold for non-inferiority of Gla + OADs versus Asp30 + MET was met, with a least squares mean change from baseline in HbA1c of -1.72% and -1.70% (-42.2 and -42.1 mmol/mol), respectively (estimated difference -0.01%; 95% CI -0.20, 0.17 [-0.1 mmol/mol; 95% CI -2.2, 1.9]). Achievement of HbA1c <7.0% (53 mmol/mol) was comparable between groups (60% vs 57%). The proportion of participants with any (24% vs 38%; p=0.003), symptomatic (19% vs 31%, p=0.007) or confirmed hypoglycaemia (18% vs 33%, p<0.001) was lower in the Gla+ OADs group.

Conclusions: Compared with Asp30 + MET, Gla +2-3 OADs showed similar efficacy but lower hypoglycaemia risk in Chinese individuals with type 2 diabetes who had undergone short-term intensive insulin therapy. This article is protected by copyright. All rights reserved.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/dom.14780DOI Listing
June 2022
-->