Publications by authors named "Liang Guo"

1,085 Publications

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Electrochemical degradation of tetracycline hydrochloride in sulfate solutions on boron-doped diamond electrode: The accumulation and transformation of persulfate.

Chemosphere 2022 Jun 25:135448. Epub 2022 Jun 25.

College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, China; Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, 266100, China.

In this study, a novel electrifying mode (divided power-on and power-off stage) was applied in the system of BDD activate sulfate to degrade tetracycline hydrochloride (TCH). The BDD electrode could activate sulfate and HO to generate sulfate radicals (SO) and hydroxyl radicals (OH) to remove TCH, and SO could dimerize to form SO. Then, the SO was activated by heat and quinones to generate SO for the continuous degradation of TCH during the power-off stage. In addition, the intermittent time has a significant effect on the degradation of TCH. Factors, affecting the accumulation of SO, were analyzed using a full factorial design, and the accumulation of SO could reach 16.2 mM in 120 min. The results of electron spin resonance and radical quenching test showed that SO, OH, direct electron transfer (DET), and non-radical in the system could effectively degrade TCH, and SO was dominated. The intermediate products of TCH were analyzed by HPLC-QTOF-MS/MS, and the TCH mainly underwent hydroxylation, demethylation and ring opening reactions to form small molecules, and finally mineralized. The results of the feasibility analysis revealed that some intermediates have high toxicity, but the system could improve the toxicity. The results of energy consumption indicated that the intermittent electrifying mode could make full use of the persulfate generated during the power-on stage and reduce about 30% energy consumption. In conclusion, this work demonstrated that it was economically feasible to degrade TCH in wastewater by activating sulfate with BDD electrodes with an intermittent electrifying mode.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135448DOI Listing
June 2022

Efficient Production of L-homophenylalanine by  Enzymatic-Chemical Cascade Catalysis.

Angew Chem Int Ed Engl 2022 Jun 28. Epub 2022 Jun 28.

Jiangnan University, State Key Laboratory of Food Science and Technology, 1800 Lihu Road, Wuxi, China, 214122, Wuxi, CHINA.

L-Homophenylalanine (L-HPA) is a vital building block for the synthesis of numerous chiral drugs. However, the high cost of starting materials limits the industrial production of L-HPA. In this study, an enzymatic-spontaneous chemical cascade route for L-HPA production was designed based on retrosynthetic analysis. This route, using simple benzaldehyde and pyruvate as starting materials, is extremely cost-effective. The enzymes were screened and further assembled in E.coli, and TipheDH was identified as the rate-limiting enzyme. Therefore, TipheDH was engineered to improve its specific activity (by 82%) and expression level (by 254%), thus generating the best strain (W14). W14 exhibited the optimum enzyme activity ratio (1.7:1.1:1:1.8) and demonstrated production of 100.9 g/L of L-HPA (with 94% conversion, >99% ee) in a 5-L reactor. This route effectively exploits the power of cascades and offers insight into avenues for synthesizing other valuable chemicals from inexpensive building blocks.
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http://dx.doi.org/10.1002/anie.202207077DOI Listing
June 2022

Higd1a facilitates exercise-mediated alleviation of fatty liver in diet-induced obese mice.

Metabolism 2022 Jun 21:155241. Epub 2022 Jun 21.

School of Kinesiology, Shanghai University of Sport, Shanghai 200438, PR China; Shanghai Frontiers Science Research Base of Exercise and Metabolic Health, Shanghai University of Sport, Shanghai 200438, PR China. Electronic address:

Background: Nonalcoholic fatty liver disease (NAFLD) has emerged as the most common liver disease. Exercise is an effective strategy against NAFLD, but its underlying molecular mechanism is not completely understood.

Methods: Higd1a, a mitochondrial inner membrane protein, was knocked down or overexpressed in mice livers by tail vein injection of adeno-associated virus (AAV) vectors. High fat diet-induced obese mice were subjected to treadmill training. Alpha mouse liver 12 (AML12) cells were used for in vitro studies.

Results: Higd1a was upregulated in mice livers after treadmill exercise training. Knockdown of Higd1a in diet-induced obese mice livers impaired exercise-mediated alleviation of hepatic steatosis, liver injury and inflammation. On the contrary, hepatic overexpression of Higd1a ameliorated fatty liver, liver injury and inflammation in synergy with exercise. Mechanistically, deficiency of Higd1a in hepatocytes promoted free fatty acids (FFAs)-induced apoptosis and oxidative stress, and elevated the cytosolic level of oxidized mitochondrial DNA (ox-mtDNA) to activate NLRP3 inflammasome and JNK signaling, leading to decreased expression of critical genes involved in fatty acid oxidation (FAO), such as Ppara, Cpt1a and Acadm. Overexpression of Higd1a in hepatocytes blunted the above effects, which ultimately increased FAO genes expression and alleviated fat accumulation in hepatocytes.

Conclusion: These results identify a Higd1a-mediated inhibition of cytosolic ox-mtDNA/NLRP3 inflammasomes/JNK pathway that facilitates exercise-mediated alleviation of hepatosteatosis.
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http://dx.doi.org/10.1016/j.metabol.2022.155241DOI Listing
June 2022

FOXM1-mediated NUF2 expression confers temozolomide resistance to human glioma cells by regulating autophagy via the PI3K/AKT/mTOR signaling pathway.

Neuropathology 2022 Jun 14. Epub 2022 Jun 14.

Department of Neurosurgery, Tongde Hospital of Zhejiang Province, Hangzhou, China.

Glioma is the most common malignant tumor in the central nervous system and has a high mortality rate. Temozolomide (TMZ) is a widely used chemotherapeutic drug for glioma. NDC80 kinetochore complex (NUF2) is suggested to play a regulatory role in different cancers, but its specific function and mechanism in glioblastoma TMZ resistance remain unknown. NUF2, assessed by reverse transcription quantitative polymerase chain reaction (RT-qPCR), was highly expressed in glioma cell lines. TMZ was used to treat cells to establish a TMZ-resistant cell line. The potential functions of NUF2 in glioma were assessed using cell counting kit-8 (CCK-8) assays, colony formation assays, 5-Ethynyl-2'-deoxyuridine (EdU) assays, flow cytometry, Western blotting, and a tumor xenograft model. The results showed that NUF2 knockdown attenuated malignant phenotypes of TMZ-resistant cells and prevented tumor growth. Mechanistically, as luciferase reporter assays and chromatin immunoprecipitation (ChIP) as showed, Fox transcription factor M1 (FOXM1) had binding sites on the NUF2 promoter. Rescue assays demonstrated that FOXM1 upregulation counteracted the inhibitory effects of NUF2 depletion on the malignancies of TMZ-resistant cells. This study demonstrates that FOXM1-activated NUF2 promotes TMZ to human glioma cells by regulating proliferation, apoptosis, and autophagy.
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http://dx.doi.org/10.1111/neup.12824DOI Listing
June 2022

Insights into the response of anammox sludge to the combined stress of nickel and salinity.

Sci Total Environ 2022 Jun 11;842:156670. Epub 2022 Jun 11.

Department of Environmental Engineering, College of Environmental & Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address:

Anaerobic ammonium oxidation (anammox) is a promising technology applied to treat industrial wastewater, while the commonly coexistent heavy metals and salinity usually become a challenging issue to be addressed. In this study, the responses of anammox sludge in terms of performance, activity, functional enzyme and extracellular polymeric substance (EPS) to the combined stress of Ni(II) and salinity (20 ‰) were investigated holistically. It turned out that low Ni(II) concentration (0.2 mg·L) together with salinity (20 ‰) showed an insignificant effect on the anammox performance, while a decreased nitrogen removal by 46.96 % was observed with the increased Ni(II) concentration to 1 mg·L. It should be pointed out that the anammox system exhibited good robustness evidenced by rapid recovery to achieve 89.13 % of nitrogen removal efficiency and 1.21 kg·m·d of nitrogen removal rate after the elimination of stress factors within 40 days. Ni(II) concentration was revealed to play a more important role in the specific activity of anammox sludge. The functional enzymes related to nitrogen removal, e.g. nitrite reductase (NIR), hydrazine oxidase (HZO) and heme c were found to be inhibited by the combined stress of Ni(II) and salinity, with decreased activity by 49.54 %, 39.39 % and 45.88 %, respectively. However, the enzyme related to assimilation, e.g. alkaline phosphatase (AKP) and nitrate reductase (NAR) appeared to be enhanced. The EPS content was found to decrease by 55.19 % under the combined stress. Detailed analysis of 3D-EEM and FTIR spectra further revealed that the combined stress of Ni(II) and salinity could change both the quantity and composition of EPS in anammox sludge. These results are expected to offer insights into the combined effect of nickel and salinity on the anammox system, and benefit the application of anammox technology for industrial metal-rich saline wastewater treatment.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156670DOI Listing
June 2022

Associations between sleep duration, sleep quality, and weight status in Chinese children and adolescents.

BMC Public Health 2022 Jun 7;22(1):1136. Epub 2022 Jun 7.

School of Physical Education and Sport Training, Shanghai University of Sport, Yangpu District, Changhai Road No 399, Shanghai, 200438, China.

Background And Objective: The high prevalence of obesity is a serious problem, and sleep is considered to be a factor for obesity. This study aimed to examine the relationship between sleep duration, sleep quality, and weight status among children and adolescents in China and to explore whether the relationship between sleep duration and weight status is independent of sleep quality. Sex and age differences were also explored.

Methods: A cross-sectional research was conducted among 2019 children and adolescents (1029 boys and 990 girls) aged 8-16 years in Shanghai. An open-question was used to obtain data on sleep duration, which was categorized into the following four groups based on the age-specific National Sleep Foundation Sleep Duration Recommendations: 1) very short, 2) short, 3) recommended, and 4) long. The Pittsburgh Sleep Quality Index was used to assess sleep quality. Weight and height were measured for all participants. The variable weight status was estimated with the Chinese children and adolescent age- and sex-specific body mass index (BMI) and was categorized into overweight/obesity and normal weight.

Results: Short sleep duration (7-8 and 6-7 h for 6-13 and 14-16 years old, respectively) significantly increased odds of overweight/obesity (OR = 1.32, 95% CI: 1.06-1.64) compared with that of the recommended sleep duration (9-11 and 8-10 h for 6-13 and 14-16 years old, respectively). The relationship between the two variables existed independent of sleep quality. No significant relationship was found between sleep quality and overweight/obesity of children and adolescents. Sleep quality (OR = 1.07, 95% CI: 1.01-1.14) and short sleep duration (OR = 1.51, 95% CI: 1.06-2.13) increased the risk for overweight/obesity among girls, whereas no significant relationships between sleep duration, sleep quality, and overweight/obesity were found among boys. Short sleep duration increased the risk of overweight/obesity in children aged 8-13 years (OR = 1.34, 95% CI: 1.05-1.71), independent of sleep quality, but no significant relationships between these two variables existed for adolescents aged 14-16 years.

Conclusions: Overall, short sleep duration increased the risks of overweight/obesity in children and adolescents in China, independent of sleep quality. This relationship is significant for girls and children aged 8-13 years instead of boys and adolescents aged 14-16 years. Interventions to extend the sleep duration of children and adolescents, especially girls and children aged 8-13 years in China, are necessary to improve their weight status.
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http://dx.doi.org/10.1186/s12889-022-13534-wDOI Listing
June 2022

Nitrogen and sulfamethoxazole removal in a partially saturated vertical flow constructed wetland treating synthetic mariculture wastewater.

Bioresour Technol 2022 Aug 31;358:127401. Epub 2022 May 31.

Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, 266100 Qingdao, China; College of Environmental Science and Engineering, Ocean University of China, 266100 Qingdao, China. Electronic address:

This study investigated the removal of nitrogen and sulfamethoxazole (SMX), and the microbial communities in a partially saturated vertical flow constructed wetland (PS-VFCW) fed with synthetic mariculture wastewater operated at different saturated zone depths (SZDs), i.e. 51, 70, and 60 cm. Removal efficiencies were 99.8%-100.0% for COD, 34.1%-100.0% for NH-N, 67.8%-97.3% for total inorganic nitrogen (TIN), and 29.8%-57.2% for SMX. Excellent nitrification performance was achieved at the SZDs of 51 and 60 cm. Denitrification performed well at 70 and 60 cm SZDs. The highest TIN removal efficiency (97.3%) was achieved as the SZD was 60 cm. SMX removal was significantly influenced by SZD and was promoted by higher SZD. The removal of organics, nitrogen, and SMX mainly occurred in the unsaturated zone. Ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, denitrifying bacteria, and SMX-degrading bacteria were detected in the unsaturated and saturated zones, and showed an increasing trend in abundance along the depth.
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http://dx.doi.org/10.1016/j.biortech.2022.127401DOI Listing
August 2022

High-throughput UAV-based phenotyping provides insights into the dynamic process and genetic basis of rapeseed waterlogging response in the field.

J Exp Bot 2022 May 31. Epub 2022 May 31.

Macro Agriculture Research Institute, College of Resource and Environment, Huazhong Agricultural University, Wuhan, China.

Waterlogging severely affects the growth, development, and yield of crops. Accurate high-throughput phenotyping is important for exploring the dynamic crop waterlogging response process in the field and the genetic basis of waterlogging tolerance. In this study, a multi-model remote sensing phenotyping platform based on an unmanned aerial vehicle (UAV) was used to assess the genetic response of rapeseed (Brassica napus) to waterlogging by measuring morphological traits and spectral indices in two years. The dynamic responses of the morphological and spectral traits indicated that the rapeseed waterlogging response was severe before the middle stage within 18 days after recovery, but it partly decreased subsequently. Genome-wide association studies identified 289 and 333 loci associated with waterlogging tolerance in two years. Next, 25 loci with at least nine associations with waterlogging-related traits were defined as highly reliable loci, and 13 loci were simultaneously identified by waterlogging tolerance coefficients of morphological traits, spectral indices, and common factors. Forty candidate genes were predicted in the regions of 13 overlapping loci. Our study provides insights into the understanding of dynamic process and genetic basis of rapeseed waterlogging response in the field by a high-throughput UAV phenotyping platform. The highly reliable loci identified in this study are valuable for breeding waterlogging-tolerant rapeseed cultivars.
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http://dx.doi.org/10.1093/jxb/erac242DOI Listing
May 2022

Critical Roles of Mitochondrial Fatty Acid Synthesis in Tomato Development and Environmental Response.

Plant Physiol 2022 May 28. Epub 2022 May 28.

Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan 430070, China.

Plant mitochondrial fatty acid synthesis (mtFAS) appears to be important in photorespiration based on the reverse genetics research from Arabidopsis (Arabidopsis thaliana) in recent years, but its roles in plant development have not been completely explored. Here, we identified a tomato (Solanum lycopersicum) mutant, fern-like, which displays pleiotropic phenotypes including dwarfism, yellowing, curly leaves, and increased axillary buds. Positional cloning, genetic and heterozygous complementation tests revealed that the underlying gene FERN encodes a 3-hydroxyl-ACP dehydratase enzyme involved in mtFAS. FERN was causally involved in tomato morphogenesis by affecting photorespiration, energy supply and the homeostasis of reactive oxygen species. Based on lipidome data, FERN and the mtFAS pathway may modulate tomato development by influencing mitochondrial membrane lipid composition and other lipid metabolic pathways. These findings provide important insights into the roles and importance of mtFAS in tomato development.
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http://dx.doi.org/10.1093/plphys/kiac255DOI Listing
May 2022

Stop-Flow Lithography for the Continuous Production of Degradable Hydrogel Achiral Crescent Microswimmers.

Micromachines (Basel) 2022 May 20;13(5). Epub 2022 May 20.

Department of Mechanical and Energy Engineering, Southern University of Science and Technology, Shenzhen 518055, China.

The small size of robotic microswimmers makes them suitable for performing biomedical tasks in tiny, enclosed spaces. Considering the effects of potentially long-term retention of microswimmers in biological tissues and the environment, the degradability of microswimmers has become one of the pressing issues in this field. While degradable hydrogel was successfully used to prepare microswimmers in previous reports, most hydrogel microswimmers could only be fabricated using two-photon polymerization (TPP) due to their 3D structures, resulting in costly robotic microswimmers solution. This limits the potential of hydrogel microswimmers to be used in applications where a large number of microswimmers are needed. Here, we proposed a new type of preparation method for degradable hydrogel achiral crescent microswimmers using a custom-built stop-flow lithography (SFL) setup. The degradability of the hydrogel crescent microswimmers was quantitatively analyzed, and the degradation rate in sodium hydroxide solution (NaOH) of different concentrations was investigated. Cytotoxicity assays showed the hydrogel crescent microswimmers had good biocompatibility. The hydrogel crescent microswimmers were magnetically actuated using a 3D Helmholtz coil system and were able to obtain a swimming efficiency on par with previously reported microswimmers. The results herein demonstrated the potential for the degradable hydrogel achiral microswimmers to become a candidate for microscale applications.
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http://dx.doi.org/10.3390/mi13050798DOI Listing
May 2022

Rational Design of Phospholipase D to Improve the Transphosphatidylation Activity for Phosphatidylserine Synthesis.

J Agric Food Chem 2022 Jun 26;70(22):6709-6718. Epub 2022 May 26.

School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China.

Phosphatidylserine (PS) has been widely used in the fields of food and medicine, among others, owing to its unique chemical structure and health benefits. However, the phospholipase D (PLD)-mediated enzymatic production of PS remains a challenge due to the low transphosphatidylation activity of PLD. Therefore, in the present study, we designed a maltose-binding protein (MBP) tag and a PLD co-expression method to achieve the expression of soluble PLD in . A "reconstruct substrate pocket" strategy was then proposed based on the catalytic mechanism and molecular dynamics simulation, expanding the substrate pocket and manipulating the coordination of l-Ser within the active site. The best mutant (PLD) exhibited a 2.04-fold higher transphosphatidylation/hydrolysis ratio than the wild-type Furthermore, under optimal conditions, Mu produced 58.6 g/L PS with 77.2% conversion, within 12 h on a 3 L scale, which demonstrates the potential of the proposed method for industrial application.
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http://dx.doi.org/10.1021/acs.jafc.2c02212DOI Listing
June 2022

Transcriptional regulation of oil biosynthesis in seed plants: Current understanding, applications, and perspectives.

Plant Commun 2022 Apr 20:100328. Epub 2022 Apr 20.

School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore. Electronic address:

Plants produce and accumulate triacylglycerol (TAG) in their seeds as an energy reservoir to support the processes of seed germination and seedling development. Plant seed oils are vital not only for the human diet but also as renewable feedstocks for industrial use. TAG biosynthesis consists of two major steps: de novo fatty acid biosynthesis in the plastids and TAG assembly in the endoplasmic reticulum. The latest advances in unraveling transcriptional regulation have shed light on the molecular mechanisms of plant oil biosynthesis. We summarize recent progress in understanding the regulatory mechanisms of well-characterized and newly discovered transcription factors and other types of regulators that control plant fatty acid biosynthesis. The emerging picture shows that plant oil biosynthesis responds to developmental and environmental cues that stimulate a network of interacting transcriptional activators and repressors, which in turn fine-tune the spatiotemporal regulation of the pathway genes.
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http://dx.doi.org/10.1016/j.xplc.2022.100328DOI Listing
April 2022

GLI1 is involved in HIF-1α-induced migration, invasion, and epithelial-mesenchymal transition in glioma cells.

Authors:
Yihai Lin Liang Guo

Folia Histochem Cytobiol 2022 23;60(2):156-166. Epub 2022 May 23.

Department of Neurosurgery, Tongde Hospital of Zhejiang Province, Zhejiang, China.

Introduction: Glioma is characterized by hypoxia that activates the hypoxia inducible factor (HIF) pathway and controls a myriad of genes that drive cancer progression. HIF-1α promotes GLI1 transferring to the nucleus by activating the hedgehog pathway under hypoxic conditions. However, their mechanisms in glioma cells under hypoxia remain unknown.

Material And Methods: Human glioma cell lines (LN229 and LN18) were transfected with HIF-1α or GLI1-specific short hairpin RNAs (shRNAs) and cultured under normoxic or hypoxic conditions. The protein levels of HIF-1α, GLI1, and epithelial-mesenchymal transition (EMT) markers including E-cadherin and vimentin were measured by Western blot analysis. RT-qPCR analysis was performed for the detection of HIF-1α and GLI1 mRNA expression. Cell migratory and invasive capacities were evaluated by wound healing and Transwell assays, respectively.

Results: Hypoxia blocked the breakdown of the HIF-1α protein and upregulated GLI1 expression in glioma cells. Downregulation of HIF-1α expression inhibited hypoxia-induced cell migration and invasion, as well as reversed the effects of hypoxia on GLI1, E-cadherin, and vimentin expression in LN229 and LN18 cells. Depletion of GLI1 inhibited glioma cell migration and invasion induced by hypoxia. Silenced GLI1 did not affect HIF-1α expression but completely offset hypoxia-regulated expression of E-cadherin and vimentin in glioma cells.

Conclusions: GLI1 is involved in HIF-1α-induced migration, invasion, and EMT in glioma cells, thus revealing a novel molecular mechanism for glioma research.
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http://dx.doi.org/10.5603/FHC.a2022.0014DOI Listing
July 2022

Current state and future perspectives of cytochrome P450 enzymes for C-H and C=C oxygenation.

Synth Syst Biotechnol 2022 Sep 8;7(3):887-899. Epub 2022 May 8.

School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, China.

Cytochrome P450 enzymes (CYPs) catalyze a series of C-H and C=C oxygenation reactions, including hydroxylation, epoxidation, and ketonization. They are attractive biocatalysts because of their ability to selectively introduce oxygen into inert molecules under mild conditions. This review provides a comprehensive overview of the C-H and C=C oxygenation reactions catalyzed by CYPs and the various strategies for achieving higher selectivity and enzymatic activity. Furthermore, we discuss the application of C-H and C=C oxygenation catalyzed by CYPs to obtain the desired chemicals or pharmaceutical intermediates in practical production. The rapid development of protein engineering for CYPs provides excellent biocatalysts for selective C-H and C=C oxygenation reactions, thereby promoting the development of environmentally friendly and sustainable production processes.
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http://dx.doi.org/10.1016/j.synbio.2022.04.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112060PMC
September 2022

Genome-Wide Analysis of Soybean Lateral Organ Boundaries Domain Gene Family Reveals the Role in Root and Stem Rot.

Front Plant Sci 2022 4;13:865165. Epub 2022 May 4.

Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, China.

The plant-specific lateral organ boundaries (LOB) domain (LBD) proteins, a family of transcription factors, play important roles in plant growth and development, as well as in responses to various stresses. However, little is known about the functions of genes in soybean (). In this study, we investigated the evolution and classification of the LBD family in soybean by a phylogenetic tree of the gene family from 16 species. Phylogenetic analysis categorized these proteins into two classes (Class I and Class II) with seven subgroups. Moreover, we found that all the 18 ancestors in angiosperm were kept in soybean, common bean genomes, and genome-wide duplication, suggesting the main force for the expansion of LBD from common bean to soybean. Analysis of gene expression profiling data indicated that 16 genes were significantly induced at different time points after inoculation of soybean plants (cv. Huachun 6) with (). We further assessed the role of four highly upregulated genes, , , , and , in plant defense in soybean hairy roots using the transient overexpression and knockdown assays. The results showed that and negatively regulate plant immunity against , whereas and positively manipulate plant immunity against . Collectively, our findings expand our knowledge of the origin and evolution of the gene family in soybean and promote the potential application of these genes in soybean genetic improvement.
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http://dx.doi.org/10.3389/fpls.2022.865165DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9116278PMC
May 2022

Assessment of the Novel, Practical, and Prognosis-Relevant TNM Staging System for Stage I-III Cutaneous Melanoma.

Front Oncol 2022 29;12:738298. Epub 2022 Apr 29.

Department of Plastic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, China.

Background: The clinical TNM staging system does not differ between the 7 and 8 editions of the American Joint Committee on Cancer (AJCC) staging manual. A more practical TNM staging system for patients with stage I-III cutaneous melanoma are needed.

Methods: Data were accessed from the Surveillance, Epidemiology, and End Results (SEER) open database. We divided the patients into 32 groups based on the T and N categories. The Kaplan-Meier survival curves and treatment guidelines were used to proposed a new TNM staging system. Cox proportional hazards model and 1000-person-years were used to verify accuracy.

Results: This retrospective study included 68 861 patients from 2010 to 2015. The new proposed staging system was as follows: stage IA, T1aN0M0; stage IB, T1b/T2aN0M0; stage IIA, T3-4aN0M0 and T2bN0M0; stage IIB, T1-4aN1-2M0 and T3-4bN0M0; and stage III, T1-4aN3M0 and T1-4bN1-3M0. Hazard ratios for the new stages IB, IIA, IIB, and III, with stage IA as reference, were 4.311 (95% confidence interval [CI]: 3.217-5.778), 8.993 (95% CI: 6.637-12.186), 13.179 (95% CI: 9.435-18.407), and 20.693 (95% CI: 13.655-31.356), respectively (all p-values < 0.001). Cancer-specific mortality rates per 1000-person-years were 0.812 (95% CI: 0.674-0.978), 6.612 (95% CI: 5.936-7.364), 22.228 (95% CI: 20.128-24.547), 50.863 (95% CI: 47.472-54.496) and 120.318 (95% CI: 112.596-128.570) for stages IA, IB, IIA, IIB and III, respectively.

Conclusion: We developed a more practical and prognosis-relevant staging system than that of the 8 edition AJCC manual for patients with stage I-III cutaneous melanoma. Treatments using this new model would improve the quality of life and survival rates of patients with melanoma.
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http://dx.doi.org/10.3389/fonc.2022.738298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9104117PMC
April 2022

Genome-Wide Association Studies of Salt-Alkali Tolerance at Seedling and Mature Stages in .

Front Plant Sci 2022 27;13:857149. Epub 2022 Apr 27.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.

Most plants are sensitive to salt-alkali stress, and the degree of tolerance to salt-alkali stress varies from different species and varieties. In order to explore the salt-alkali stress adaptability of , we collected the phenotypic data of 505 accessions at seedling and mature stages under control, low and high salt-alkali soil stress conditions in Inner Mongolia of China. Six resistant and 5 sensitive materials, respectively, have been identified both in Inner Mongolia and Xinjiang Uygur Autonomous Region of China. Genome-wide association studies (GWAS) for 15 absolute values and 10 tolerance coefficients (TCs) of growth and agronomic traits were applied to investigate the genetic basis of salt-alkali tolerance of . We finally mapped 9 significant QTLs related to salt-alkali stress response and predicted 20 candidate genes related to salt-alkali stress tolerance. Some important candidate genes, including , and , were identified by combining sequence variation annotation and expression differences. The identified valuable loci and germplasms could be useful for breeding salt-alkali-tolerant varieties. This study laid a foundation for understanding molecular mechanism of salt-alkali stress adaptation and provides rich genetic resources for the large-scale production of on salt-alkali land in the future.
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http://dx.doi.org/10.3389/fpls.2022.857149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9094488PMC
April 2022

Roles of illumination on distribution of phosphorus in Chlorella vulgaris under mixotrophic cultivation.

Chemosphere 2022 Sep 10;303(Pt 1):134904. Epub 2022 May 10.

College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, China.

Phosphorus (P) is a non-substitutable resource and global reserves of phosphate rock are limited. In this study, phosphorus recovery by Chlorella vulgaris, and the effects of different light intensities (2000 Lux, 5000 Lux, 8000 Lux, 12,000 Lux) on the phosphorus distribution in the soluble microbial product (SMP), extracellular polymeric substance (EPS) and intracellular polymeric substance (IPS) were analyzed. The results showed that the 5000 Lux was the optimum light intensity for P uptake and transformation by Chlorella vulgaris under mixotrophic cultivation. At the light intensity of 5000 Lux, the P uptake rate was 100% after 32 days of cultivation, and the concentration of intracellular organic phosphorus (OP) was 5.77 mg P/L. Moreover, EPS was the main P pool when inorganic phosphorus (IP) was depleted in bulk solution. Phosphorus recovery by microalgae is an important solution to treat P-containing wastewater.
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http://dx.doi.org/10.1016/j.chemosphere.2022.134904DOI Listing
September 2022

Oil plant genomes: current state of the science.

J Exp Bot 2022 May;73(9):2859-2874

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.

Vegetable oils are an indispensable nutritional component of the human diet as well as important raw materials for a variety of industrial applications such as pharmaceuticals, cosmetics, oleochemicals, and biofuels. Oil plant genomes are highly diverse, and their genetic variation leads to a diversity in oil biosynthesis and accumulation along with agronomic traits. This review discusses plant oil biosynthetic pathways, current state of genome assembly, polyploidy and asymmetric evolution of genomes of oil plants and their wild relatives, and research progress of pan-genomics in oil plants. The availability of complete high-resolution genomes and pan-genomes has enabled the identification of structural variations in the genomes that are associated with the diversity of agronomic and environment fitness traits. These and future genomes also provide powerful tools to understand crop evolution and to harvest the rich natural variations to improve oil crops for enhanced productivity, oil quality, and adaptability to changing environments.
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http://dx.doi.org/10.1093/jxb/erab472DOI Listing
May 2022

Prevalence of intracranial hemorrhage amongst patients presenting with out-of-hospital cardiac arrest: A systematic review and meta-analysis.

Resuscitation 2022 Jul 10;176:136-149. Epub 2022 May 10.

Department of Emergency Medicine, Singapore General Hospital, Singapore; Pre-hospital and Emergency Research Centre, Duke-NUS Medical School, Singapore. Electronic address:

Introduction: An unknown proportion of out-of-hospital cardiac arrest (OHCA) is caused by intracranial hemorrhage (ICH). There is uncertainty over the role of early head computed tomography (CT) in non-traumatic OHCA due to uncertain diagnostic yield and ways to identify high-risk patients. This study aimed to identify the prevalence of ICH in non-traumatic OHCA and possible predictors.

Methods: PubMed, EMBASE, and the Cochrane library were searched from inception to January 2022. Data extraction and quality assessment were independently reviewed by two authors. Meta-analyses estimated the prevalence of ICH amongst OHCA patients and pre-specified subgroups and geographical settings. Subgroup analysis were used to explore potential clinical predictors.

Results: 23 studies involving 54,349 patients were included. The pooled ICH prevalence was 4.28% (95%CI: 3.31-5.24). Asia had a significantly larger risk ratio (RR = 3.93, P value < 0.0001) than Europe. The ICH subgroup was significantly more likely to be female (OR: 2.16; 95%CI: 1.10-4.26), and less likely to experience shockable rhythms compared with non-shockable rhythms (OR: 0.22; 95% CI: 0.04-1.22), achieve ROSC prior to arrival (OR: 0.27; 95%CI: 0.10-0.77), and survive to discharge compared to those without ICH (OR: 0.26; 95%CI: 0.11-0.59).

Conclusions: One in twenty OHCA have ICH at the time of presentation. An early head CT scan should be strongly considered after return of spontaneous circulation (ROSC), especially in patients who are female, with non-shockable rhythm and did not attain ROSC prior to arrival. These finding should influence clinical protocols to favor routine scans especially in Asia where prevalence is higher.
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http://dx.doi.org/10.1016/j.resuscitation.2022.05.001DOI Listing
July 2022

Mesh-like electrospun membrane loaded with atorvastatin facilitates cutaneous wound healing by promoting the paracrine function of mesenchymal stem cells.

Stem Cell Res Ther 2022 05 7;13(1):190. Epub 2022 May 7.

Department of Plastic Surgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.

Background: Functional electrospun membranes are promising dressings for promoting wound healing. However, their microstructure and drug loading capacity need further improvements. It is the first time to design a novel mesh-like electrospun fiber loaded with atorvastatin (ATV) and investigated its effects on paracrine secretion by bone marrow-derived mesenchymal stem cells (BMSCs) and wound healing in vivo.

Methods: We fabricated a mesh-like electrospun membrane using a copper mesh receiver. The physical properties of the membranes were evaluated by SEM, FTIR spectroscopy, tensile strength analysis, and contrast angle test. Drug release was measured by plotting concentration as a function of time. We tested the effects of conditioned media (CM) derived from BMSCs on endothelial cell migration and angiogenesis. We used these BMSCs and performed RT-PCR and ELISA to evaluate the expressions of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF) genes and proteins, respectively. The involvement of FAK and AKT mechanotransduction pathways in the regulation of BMSC secretion by material surface topography was also investigated. Furthermore, we established a rat model of wound healing, applied ATV-loaded mesh-like membranes (PCL/MAT) seeded with BMSCs on wounds, and assessed their efficacy for promoting wound healing.

Results: FTIR spectroscopy revealed successful ATV loading in PCL/MAT. Compared with random electrospun fibers (PCL/R) and mesh-like electrospun fibers without drug load (PCL/M), PCL/MAT induced maximum promotion of human umbilical vein endothelial cell (HUVEC) migration. In the PCL/MAT group, the cell sheet scratches were nearly closed after 24 h. However, the cell sheet scratches remained open in other treatments at the same time point. The PCL/MAT promoted angiogenesis and led to the generation of longer tubes than the other treatments. Finally, the PCL/MAT induced maximum gene expression and protein secretion of VEGF and b-FGF. As for material surface topography effect on BMSCs, FAK and AKT signaling pathways were shown to participate in the modulation of MSC morphology and its paracrine function. In vivo, PCL/MAT seeded with BMSCs significantly accelerated healing and improved neovascularization and collagen reconstruction in the wound area compared to the other treatments.

Conclusions: The mesh-like topography of fibrous scaffolds combined with ATV release creates a unique microenvironment that promotes paracrine secretion of BMSCs, thereby accelerating wound healing. Hence, drug-loaded mesh-like electrospun membranes may be highly efficacious for wound healing and as artificial skin. It is a promising approach to solve the traumatic skin defect and accelerate recovery, which is essential to developing functional materials for future regenerative medicine.
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http://dx.doi.org/10.1186/s13287-022-02865-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080129PMC
May 2022

Comparison of primary and secondary sludge carbon sources derived from hydrolysis or acidogenesis for nitrate reduction and denitrification kinetics: Organics utilization and microbial community shift.

Environ Res 2022 Sep 5;212(Pt C):113403. Epub 2022 May 5.

Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada; Department of Environmental Science, Baylor University, Waco, TX, USA.

Seeking available and economical carbon sources for denitrification process is an intractable issue for wastewater treatment. However, no study compared different types of waste sludge as carbon source from denitrification mechanism, organics utilization and microbial community aspects. In this study, primary and secondary sludge were pretreated by thermophilic bacteria (TB), and its hydrolysis or acidogenic liquid were prepared as carbon sources for denitrification. At C/N of 8-3, the variations of NO-N and NO-N were profiled in typical cycles and denitrification kinetics was analyzed. Primary sludge achieved a competitive NO-N removal efficiency with less dosage than secondary sludge. Fourier transform infrared (FTIR) spectroscopy was introduced to analyze organic composition from functional-group perspective and the utilization of organic matters in different sludge carbon sources was investigated. To further analyze the microbial community shift in different denitrification systems, high-throughput sequencing technology was applied. Results showed that denitrifier Thauera, belonging to Proteobacteria, was predominant, and primary sludge acidogenic liquid enriched Thauera most intensively with relative abundance of 47.3%.
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http://dx.doi.org/10.1016/j.envres.2022.113403DOI Listing
September 2022

Application of Visual Sensing Techniques in Computational Intelligence for Risk Assessment of Sports Injuries in Colleges.

Comput Intell Neurosci 2022 22;2022:9080661. Epub 2022 Apr 22.

Department of Physical Education, Chang'an University, Xi'an, Shaanxi 710064, China.

Injury prediction is one of the most challenging issues in sports and is a key component of injury prevention, since successful injury prediction forms the basis for effective preventive measures. In this study, an analysis was made on the risk of physical injuries to college students to guarantee the physical safety of students in sports and improve the quality of physical education. Then, a study was carried out on the occurrences of physical injury risks through visual sensing techniques, and an investigation was conducted into the characteristics of physical injury risks in colleges. Next, the student's body shape and physical characteristics are computed using visual sensing techniques, and the risk of sports injuries is evaluated. The results show that the proposed image recognition and computation methods can accurately identify the sports injuries of college students. Furthermore, it can effectively analyze the factors affecting the risk of sports injuries, and the error of the proposed technique remains between -3 and 2. In addition, it can accurately locate the occurrence of sports injury risks and reduce the impact of those risks in time. This work provides technical support for the reduction of sports injury risks and contributes to the improvement of physical education teaching quality in colleges.
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http://dx.doi.org/10.1155/2022/9080661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054421PMC
May 2022

Nonlinearity-mediated collimation of optical beams.

Opt Express 2022 Mar;30(7):10770-10778

We investigated the evolutions of optical beams in an optical system composed of free spaces and nonlocal nonlinear media layers in a cascaded manner. From an application point of view, two kinds of evolution processes for Gaussian beams, nonlinearity-mediated collimation and switching from breathers to solitons, were discussed in details. By adjusting the input optical power, the collimating, the compressing and the expanding of optical beams are convenient to be controlled.
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http://dx.doi.org/10.1364/OE.455935DOI Listing
March 2022

[Advances in synthetic biology of CO fixation by heterotrophic microorganisms].

Sheng Wu Gong Cheng Xue Bao 2022 Apr;38(4):1339-1350

State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.

Human activities increase the concentration of atmospheric carbon dioxide (CO), which leads to global climate warming. Microbial CO fixation is a promising green approach for carbon neutral. In contrast to autotrophic microorganisms, heterotrophic microorganisms are characterized by fast growth and ease of genetic modification, but the efficiency of CO fixation is still limited. In the past decade, synthetic biology-based enhancement of heterotrophic CO fixation has drawn wide attention, including the optimization of energy supply, modification of carboxylation pathway, and heterotrophic microorganisms-based indirect CO fixation. This review focuses on the research progress in CO fixation by heterotrophic microorganisms, which is expected to serve as a reference for peaking CO emission and achieving carbon neutral by microbial CO fixation.
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http://dx.doi.org/10.13345/j.cjb.210627DOI Listing
April 2022

DGInet: Dynamic graph and interaction-aware convolutional network for vehicle trajectory prediction.

Neural Netw 2022 Jul 4;151:336-348. Epub 2022 Apr 4.

College of Computer Science and Electronic Engineering, Hunan University, Changsha 410082, China.

This paper investigates vehicle trajectory prediction problems in real traffic scenarios by fully harnessing the spatio-temporal dependencies between multiple vehicles. The existing GCN-based trajectory predictions are often considered in a single traffic scene without time attributes, complete interaction information, dynamic graph-based model, etc. Time and interaction aware models are more challenging than the existing ones. Despite very well does the graph-based model describe the relationship between driving vehicles, the critical problem in the traffic scene is how to deeply explore the spatio-temporal characteristics of dynamic graphs. Therefore, a novel dynamic graph and interaction-aware neural network model called as DGInet is proposed by combining a semi-global graph mechanism and an M-product based graph convolutional network, which are built into novel dual-network architecture in the entire model. The DGInet is built by exploiting the dynamic interaction in depth between driving vehicles in urban traffic scenarios, and then realized by utilizing semi-global graph convolution operations on the input data cell to capture the basic spatial interaction features of the driving scene. Meanwhile, the dynamic graph is further extracted by a novel M-product approach, in which the embedding of the model is then established along with the embedding of the semi-global network to perform the final embedding. Extensive experiments have been conducted on the two public datasets, named NGSIM and Apollo respectively, to show that our approach outperforms the existing ones with better performance and less computing time. Besides the real-world Shenzhen traffic dataset, China, is also developed to verify the effectiveness of our approach.
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http://dx.doi.org/10.1016/j.neunet.2022.03.038DOI Listing
July 2022

DDX3X alleviates doxorubicin-induced cardiotoxicity by regulating Wnt/β-catenin signaling pathway in an in vitro model.

J Biochem Mol Toxicol 2022 Apr 25:e23077. Epub 2022 Apr 25.

Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

The life-threatening adverse effects of doxorubicin (Dox) caused by its cardiotoxic properties limit its clinical application. DDX3X has been shown to participate in a variety of physiological processes, and it acts as a regulator of Wnt/β-catenin signaling. However, the role of DDX3X in Dox-induced cardiotoxicity (DIC) remains unclear. In this study, we found that DDX3X expression was significantly decreased in H9c2 cardiomyocytes treated with Dox. Ddx3x knockdown and RK-33 (DDX3X ATPase activity inhibitor) pretreatment exacerbated cardiomyocyte apoptosis and mitochondrial dysfunction induced by Dox treatment. In contrast, Ddx3x overexpression ameliorated the DIC response. Moreover, Wnt/β-catenin signaling in cardiomyocytes treated with Dox was suppressed, but this suppression was reversed by Ddx3x overexpression. Overall, this study demonstrated that DDX3X plays a protective role in DIC by activating Wnt/β-catenin signaling.
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http://dx.doi.org/10.1002/jbt.23077DOI Listing
April 2022

Systematic trait dissection in oilseed rape provides a comprehensive view, further insight, and exact roadmap for yield determination.

Biotechnol Biofuels Bioprod 2022 Apr 19;15(1):38. Epub 2022 Apr 19.

Oil Crops Research Institute of the Chinses Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan, China.

Background: Yield is the most important and complex trait that is influenced by numerous relevant traits with very complicated interrelations. While there are a large number of studies on the phenotypic relationship and genetic basis of yield traits, systematic studies with further dissection focusing on yield are limited. Therefore, there is still lack of a comprehensive and in-depth understanding of the determination of yield.

Results: In this study, yield was systematically dissected at the phenotypic, genetic to molecular levels in oilseed rape (Brassica napus L.). The analysis of correlation, network, and principal component for 21 traits in BnaZN-RIL population showed that yield was determined by a complex trait network with key contributors. The analysis of the constructed high-density single nucleotide polymorphism (SNP) linkage map revealed the concentrated distribution of distorted and heterozygous markers, likely due to selection on genes controlling the growth period and yield heterosis. A total of 134 consensus quantitative trait loci (QTL) were identified for 21 traits, of which all were incorporated into an interconnecting QTL network with dozens of hub-QTL. Four representative hub-QTL were further dissected to the target or candidate genes that governed the causal relationships between the relevant traits.

Conclusions: The highly consistent results at the phenotypic, genetic, and molecular dissecting demonstrated that yield was determined by a multilayer composite network that involved numerous traits and genes showing complex up/down-stream and positive/negative regulation. This provides a systematic view, further insight, and exact roadmap for yield determination, which represents a significant advance toward the understanding and dissection of complex traits.
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http://dx.doi.org/10.1186/s13068-022-02134-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9019968PMC
April 2022

Insight in degradation of tetracycline in mariculture wastewater by ultraviolet/persulfate advanced oxidation process.

Environ Res 2022 Sep 16;212(Pt B):113324. Epub 2022 Apr 16.

Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering (MEGE), College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, China; Key Lab of Marine Environmental Science and Ecology (Ocean University of China), Ministry of Education, Qingdao, 266100, China. Electronic address:

The direct discharge of trace amounts of antibiotics in mariculture wastewater results in adverse effect on the ecological environment of receiving waters. Hence, the degradation of tetracycline (TC) in mariculture wastewater by the ultraviolet/peroxydisulfate (UV/PS) process was investigated in this study. The results revealed that 95.73% removal of TC with 5 mg/L dosage was achieved after 30 min UV/PS treatment. Chloride ion (Cl) in mariculture wastewater slightly inhibited TC degradation by scavenging free radicals. Comparably, bromine ion (Br) significantly enhanced the removal of TC and even doubled the degradation rate. Reactive bromine species (RBS) made a major contribution to the TC removal, followed by free chlorine and other reactive chlorine species (RCS). The TC degradation pathway revealed that functional group shedding and ring-opening reactions occurred successively. In addition, TC mineralization rate was low within 30 min, causing the inefficient reduction of acute toxicity of TC and its intermediates, which could be improved by optimizing the process parameters. These results indicated that UV/PS is a new alternative process for the harmless treatment of mariculture wastewater containing the antibiotics.
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http://dx.doi.org/10.1016/j.envres.2022.113324DOI Listing
September 2022

A method to classify bone marrow cells with rejected option.

Biomed Tech (Berl) 2022 Jun 19;67(3):227-236. Epub 2022 Apr 19.

Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Bone marrow cell morphology has always been an important tool for the diagnosis of blood diseases. Still, it requires years of experience from a suitable person. Furthermore, the outcomes of their recognition are subjective and there is no objective quantitative standard. As a result, developing a deep learning automatic classification system for bone marrow cells is extremely important. However, typical classification machine learning systems only produce classification answers, and will not refuse to generate predictions when the prediction reliability is low. It will pose a big problem in some high-risk systems such as bone marrow cell recognition. This paper proposes a bone marrow cell classification method with rejected option (CMWRO) to classify 11 bone marrow cells. CMWRO is based on convolutional neural networks, ICP and SoftMax (CNN-ICP-SoftMax), containing a classifier with rejected option. When the rejected rate (RR) of tested samples is 0.3143, it can ensure that the precision, sensitivity, accuracy of the accepted samples reach 0.9921, 0.9917 and 0.9944 respectively. And the rejected samples will be handled by other ways, such as identified by doctors. Besides, the method has a good filtering effect on cell types that the classifier is not trained, such as abnormal cells and cells with less sample distribution. It can reach more than 82% in filtering efficiency. CMWRO improves the doctors' trust in the results of accepted samples to a certain extent. They only need to carefully identify the samples that CMWRO refuses to recognize, and finally combines the two results. It can greatly improve the efficiency and accuracy of bone marrow cell recognition.
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http://dx.doi.org/10.1515/bmt-2021-0253DOI Listing
June 2022
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