Publications by authors named "Liang Dong"

1,208 Publications

  • Page 1 of 1

Synthesizing PET/MR (T1-weighted) images from non-attenuation-corrected PET images.

Phys Med Biol 2021 Jun 7. Epub 2021 Jun 7.

Paul C. Lauterbur Research Centre for Biomedical Imaging, Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences, 1068 Xueyuan Avenue, Shenzhen University Town, Shenzhen, P.R.China, Shenzhen, 518055, CHINA.

Positron emission tomography (PET) imaging can be used for early detection, diagnosis and postoperative patient monitoring of many diseases. Traditional PET imaging requires not only additional computed tomography (CT) imaging or magnetic resonance imaging (MR) to provide anatomical information but also attenuation correction (AC) map calculation based on CT images or MR images for accurate quantitative estimation. During a patient's treatment, PET/CT or PET/MR scans are inevitably repeated many times, leading to additional doses of ionizing radiation (CT scans) and additional economic and time costs (MR scans). To reduce adverse effects while obtaining high-quality PET/MR images in the course of a patient's treatment, especially in the stage of evaluating the effect of postoperative treatment, in this work, we propose a new method based on deep learning, which can directly obtain synthetic attenuation-corrected PET (sAC PET) and synthetic MR (sMR) images based only on non-attenuation-corrected PET (NAC PET) images. Our model, based on the Wasserstein generative adversarial network (WGAN), first removes noise and artifacts from the NAC PET images to generate sAC PET images and then generates sMR images from the obtained sAC PET images. To evaluate the performance of this generative model, we evaluated it on paired PET/MR images from a total of sixty clinical patients. Based on qualitative and quantitative analysis, the generated sAC PET and sMR images showed a high degree of similarity to the real AC PET and real MR images. These results indicated that our proposed method can reduce the frequency of additional anatomical imaging scans during PET imaging and has great potential in improving doctors' clinical diagnosis efficiency, saving patients' economic expenditure and reducing the radiation risk brought by CT scanning.
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http://dx.doi.org/10.1088/1361-6560/ac08b2DOI Listing
June 2021

A 3D printed Ga containing scaffold with both anti-infection and bone homeostasis-regulating properties for the treatment of infected bone defects.

J Mater Chem B 2021 Jun 6. Epub 2021 Jun 6.

Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Large bone defects face a high risk of infection, which can also lead to bone homeostasis disorders. This seriously hinders the bone healing process; therefore, the help of a dual-functional scaffold that has both anti-infection and bone-homeostasis-regulating capacities is needed in the treatment of infected bone defects. In this study, a 3D printed dual-functional scaffold composed of poly-ε-caprolactone (PCL), mesoporous bioactive glasses (MBG), and gallium (Ga) was produced. In vitro experiments demonstrated the excellent antibacterial ability of the PCL/MBG/Ga scaffold against methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli). The scaffold also significantly inhibited osteoclastic activity and promoted osteogenic differentiation. Furthermore, a rabbit model with an infected bone defect in the radius was used to evaluate the in vivo bone healing capability of PCL/MBG/Ga. The results demonstrate that the PCL/MBG/Ga scaffold can significantly accelerate bone healing and prevent bone resorption, suggesting its potential for application in repairing infected bone defects.
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http://dx.doi.org/10.1039/d1tb00387aDOI Listing
June 2021

PWLS-PR: low-dose computed tomography image reconstruction using a patch-based regularization method based on the penalized weighted least squares total variation approach.

Quant Imaging Med Surg 2021 Jun;11(6):2541-2559

Lauterbur Research Center for Biomedical Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Background: Radiation exposure computed tomography (CT) scans and the associated risk of cancer in patients have been major clinical concerns. Existing research can achieve low-dose CT imaging by reducing the X-ray current and the number of projections per rotation of the human body. However, this method may produce excessive noise and fringe artifacts in the traditional filtered back projection (FBP)-reconstructed image.

Methods: To solve this problem, iterative image reconstruction is a promising option to obtain high-quality images from low-dose scans. This paper proposes a patch-based regularization method based on penalized weighted least squares total variation (PWLS-PR) for iterative image reconstruction. This method uses neighborhood patches instead of single pixels to calculate the nonquadratic penalty. The proposed regularization method is more robust than the conventional regularization method in identifying random fluctuations caused by sharp edges and noise. Each iteration of the proposed algorithm can be described in the following three steps: image updating via the total variation based on penalized weighted least squares (PWLS-TV), image smoothing, and pixel-by-pixel image fusion.

Results: Simulation and real-world projection experiments show that the proposed PWLS-PR algorithm achieves a higher image reconstruction performance than similar algorithms. Through the qualitative and quantitative evaluation of simulation experiments, the effectiveness of the method is also verified.

Conclusions: Furthermore, this study shows that the PWLS-PR method reduces the amount of projection data required for repeated CT scans and has the useful potential to reduce the radiation dose in clinical medical applications.
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http://dx.doi.org/10.21037/qims-20-963DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107320PMC
June 2021

Contrast-enhanced CT to noncontrast CT transformation via adjacency content transfer-based deep subtraction residual neural network.

Phys Med Biol 2021 Jun 2. Epub 2021 Jun 2.

Shenzhen Institutes of Advanced Technology, shenzhen, CHINA.

To reduce overall patient radiation exposure in some clinical scenarios (i.e., cancer patients need frequent follow-ups), noncontrast CT is skipped in some institutions. However, although less desirable, noncontrast CT could provide additional important information. In this article, we propose a deep subtraction residual network based on adjacency content transfer to reconstruct noncontrast CT from contrast CT, and maintain an image quality comparable to that of originally acquired noncontrast CT scans. To address the slightly structural dissimilarity of the CT paired images (non- contrast CT and contrast CT) due to involuntarily physiological motion, we introduce a contrastive loss network derived from the adjacency content transfer strategy. We evaluated the results of various similarity metrics (MSE, SSIM, NRMSE, PSNR, MAE) and the tting curve (HU distribution) of the output mapping to estimate the recon- struction performance of the algorithm. To build the model, in total, we randomly selected 15,405 CT paired images (noncontrast CT and contrast-enhanced CT) for training and 10,270 CT paired images for testing. As a result, the proposed algorithm preserves the robust structures from contrast-enhanced CT scans and learns the non- contrast attenuation pattern from noncontrast CT scans. During the evaluation, the deep subtraction residual network achieves higher MSE, MAE, NRMSE, and PSNR scores by 30% compared to the baseline models (BEGAN, CycleGAN, Pixel2Pixel), and better simulates the HU curve of noncontrast CT attenuation. After validation based on an analysis of the experimental results, the reconstructed noncontrast CT images by our proposed algorithm not only preserve the high-quality structures from the contrast-enhanced CT images but also mimic the CT attenuation of the originally acquired noncontrast CT images.
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http://dx.doi.org/10.1088/1361-6560/ac0758DOI Listing
June 2021

FSTL1 aggravates OVA-induced inflammatory responses by activating the NLRP3/IL-1β signaling pathway in mice and macrophages.

Inflamm Res 2021 Jun 2. Epub 2021 Jun 2.

Department of Respiratory, Shandong Provincial Qianfoshan Hospital, Shandong University, The First Affiliated Hospital of Shandong First Medical University, Shandong Institute of Respiratory Diseases, Jinan, China.

Objective: Asthma, a well-known disease with high morbidity, is characterized by chronic airway inflammation. However, the allergic inflammation mechanisms of follistatin-like protein 1 (FSTL1) have not been elucidated. This study aims to investigate the effects of FSTL1 in ovalbumin (OVA)-induced mice and macrophages on nucleotide-binding domain and leucine-rich repeat protein 3 (NLRP3)/interleukin-1β (IL-1β) signaling pathway.

Methods: Mice were randomly divided into control-WT, OVA-WT, control-Fstl1, OVA-Fstl1. Histological changes were assessed by HE and PAS staining. The protein levels of Muc-5AC, FSTL1, NLRP3, and IL-1β in lung tissue were detected by immunohistochemistry and ELISA. The bronchoalveolar lavage fluid (BALF) in mice and human serum samples were detected by ELISA. Then, mice were grouped into control, FSTL1, MCC950 + FSTL1 to further investigate the relationship between FSTL1 and NLRP3/IL-1β. Alveolar macrophage cells (MH-S cells) were separated into control, OVA, FSTL1, OVA + FSTL1, OVA + siNC, OVA + siFSTL1, MCC950, and FSTL1 + MCC950 groups to explore the effect of FSTL1 on the NLRP3/IL-1β signaling. The protein expression of NLRP3 and IL-1β in MH-S cells was detected by Western blot analysis.

Results: The present results uncovered that Fstl1 significantly ameliorated OVA-induced Muc-5AC production and mucus hypersecretion. Fstl1 was also found to decrease the production of inflammatory cytokines and inflammatory cell infiltration in OVA-induced asthmatic mice. Meanwhile, the serum concentrations of FSTL1 and IL-1β were higher in  asthma subjects than the health subjects, and Fstl1 ameliorated the production of NLRP3 and IL-1β in OVA-induced asthmatic mice. Furthermore, mice by injected FSTL1 substantially stimulated the expression of NLRP3 and IL-1β, while pretreatment with MCC950 in mice significantly weakened the production of NLRP3 and IL-1β induced by injection FSTL1. Pretreatment with siFSTL1 or MCC950 significantly reduced the production of NLRP3 and IL-1β induced by OVA or FSTL1 in MH-S cells.

Conclusions: The study results showed that FSTL1 played an important role in allergic airway inflammation by activating NLRP3/IL-1β. Hence, inhibition FSTL1 could be applied as a therapeutic agent against asthma.
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http://dx.doi.org/10.1007/s00011-021-01475-wDOI Listing
June 2021

Angular sensitivity of an x-ray differential phase contrast imaging system with real and virtual source images.

Opt Lett 2021 Jun;46(11):2791-2794

In this work, a novel, to the best of our knowledge, approach based on an x-ray thin lens imaging theory is proposed to predict the angular sensitivity responses of dual-phase-grating differential phase contrast (DPC) interferometers. Experimental validations have been performed to demonstrate the high accuracy of theoretical predictions using two different setups: one with real source images and the other with virtual source images. This new sensitivity calculation method is helpful to optimize the DPC imaging performance of a dual-phase-grating system.
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http://dx.doi.org/10.1364/OL.416621DOI Listing
June 2021

Comparison of different anesthesia modalities during percutaneous kyphoplasty of osteoporotic vertebral compression fractures.

Sci Rep 2021 May 27;11(1):11102. Epub 2021 May 27.

Department of Spine Surgery, Hong-Hui Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, 710054, China.

Local and general anesthesia are the main techniques used during percutaneous kyphoplasty (PKP); however, both are associated with adverse reactions. Monitored anesthesia with dexmedetomidine may be the appropriate sedative and analgesic technique. Few studies have compared monitored anesthesia with other anesthesia modalities during PKP. Our aim was to determine whether monitored anesthesia is an effective alternative anesthetic approach for PKP. One hundred sixty-five patients undergoing PKP for osteoporotic vertebral compression fractures (OVCFs) were recruited from a single center in this prospective, non-randomized controlled study. PKP was performed under local anesthesia with ropivacaine (n = 55), monitored anesthesia with dexmedetomidine (n = 55), and general anesthesia with sufentanil/propofol/sevoflurane (n = 55). Perioperative pain was assessed using a visual analogue score (VAS). Hemodynamic variables, operative time, adverse effects, and perioperative satisfaction were recorded. The mean arterial pressure (MAP), heart rate, VAS, and operative time during monitored anesthesia were significantly lower than local anesthesia. Compared with general anesthesia, monitored anesthesia led to less adverse anesthetic effects. Monitored anesthesia had the highest perioperative satisfaction and the lowest VAS 2 h postoperatively; however, the monitored anesthesia group had the lowest MAP and heart rate 2 h postoperatively. Based on better sedation and analgesia, monitored anesthesia with dexmedetomidine achieved better patient cooperation, a shorter operative time, and lower adverse events during PKP; however, the MAP and heart rate in the monitored anesthesia group should be closely observed after surgery.
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http://dx.doi.org/10.1038/s41598-021-90621-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8159956PMC
May 2021

Hsa_circ_0092276 promotes doxorubicin resistance in breast cancer cells by regulating autophagy via miR-348/ATG7 axis.

Transl Oncol 2021 May 20;14(8):101045. Epub 2021 May 20.

Department of Breast Surgery, Henan Provincial People's Hospital, No.7 Weiwu Road, Zhengzhou 450003, Henan, China. Electronic address:

Previous study has confirmed that hsa_circ_0092276 is highly expressed in doxorubicin (DOX)-resistant breast cancer cells, indicating that hsa_circ_0092276 may be involved in regulating the chemotherapy resistance of breast cancer. Here we attempted to investigate the biological role of hsa_circ_0092276 in breast cancer. We first constructed DOX-resistant breast cancer cells (MCF-7/DOX and MDA-MB-468/DOX). The 50% inhibiting concentration of MCF-7/DOX and MDA-MB-468/DOX cells was significantly higher than that of their parental breast cancer cells, MCF-7 and MDA-MB-46. MCF-7/DOX and MDA-MB-468/DOX cells also exhibited an up-regulation of drug resistance-related protein MDR1. Compared with MCF-7 and MDA-MB-46 cells, hsa_circ_0092276 was highly expressed in MCF-7/DOX and MDA-MB-468/DOX cells. Hsa_circ_0092276 overexpression enhanced proliferation and the expression of LC3-II/LC3-I and Beclin-1, and repressed apoptosis of breast cancer cells. The effect of hsa_circ_0092276 up-regulation on breast cancer cells was abolished by 3-methyladenine (autophagy inhibitor). Hsa_circ_0092276 modulated autophagy-related gene 7 (ATG7) expression via sponging miR-384. Hsa_circ_0092276 up-regulation promoted autophagy and proliferation, and repressed apoptosis of breast cancer cells, which was abolished by miR-384 overexpression or ATG7 knockdown. In addition, LV-circ_0092276 transfected MCF-7 cell transplantation promoted autophagy and tumor growth of breast cancer in mice. In conclusion, our data demonstrate that hsa_circ_0092276 promotes autophagy and DOX resistance in breast cancer by regulating miR-348/ATG7 axis. Thus, this article highlights a novel competing endogenous RNA circuitry involved in DOX resistance in breast cancer.
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http://dx.doi.org/10.1016/j.tranon.2021.101045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163983PMC
May 2021

Berry Curvature Effects on Quasiparticle Dynamics in Superconductors.

Phys Rev Lett 2021 May;126(18):187001

Department of Physics, The University of Texas at Austin, Austin, Texas 78712, USA.

We construct a theory for the semiclassical dynamics of superconducting quasiparticles by following their wave packet motion and reveal rich contents of Berry curvature effects in the phase space spanned by position and momentum. These Berry curvatures are traced back to the characteristics of superconductivity, including the nontrivial momentum-space geometry of superconducting pairing, the real-space supercurrent, and the charge dipole of quasiparticles. The Berry-curvature effects strongly influence the spectroscopic and transport properties of superconductors, such as the local density of states and the thermal Hall conductivity. As a model illustration, we apply the theory to study the twisted bilayer graphene with a d_{x^{2}+y^{2}}+id_{xy} superconducting gap function and demonstrate Berry-curvature induced effects.
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http://dx.doi.org/10.1103/PhysRevLett.126.187001DOI Listing
May 2021

Homotopic Gradients of Generative Density Priors for MR Image Reconstruction.

IEEE Trans Med Imaging 2021 May 19;PP. Epub 2021 May 19.

Deep learning, particularly the generative model, has demonstrated tremendous potential to significantly speed up image reconstruction with reduced measurements recently. Rather than the existing generative models that often optimize the density priors, in this work, by taking advantage of the denoising score matching, homotopic gradients of generative density priors (HGGDP) are exploited for magnetic resonance imaging (MRI) reconstruction. More precisely, to tackle the low-dimensional manifold and low data density region issues in generative density prior, we estimate the target gradients in higher-dimensional space. We train a more powerful noise conditional score network by forming high-dimensional tensor as the network input at the training phase. More artificial noise is also injected in the embedding space. At the reconstruction stage, a homotopy method is employed to pursue the density prior, such as to boost the reconstruction performance. Experiment results implied the remarkable performance of HGGDP in terms of high reconstruction accuracy. Only 10% of the k-space data can still generate image of high quality as effectively as standard MRI reconstructions with the fully sampled data.
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http://dx.doi.org/10.1109/TMI.2021.3081677DOI Listing
May 2021

The efficacy of ultrasound-guided stellate ganglion block in alleviating postoperative pain and ventricular arrhythmias and its application prospects.

Neurol Sci 2021 May 18. Epub 2021 May 18.

Department of Anesthesiology, Affiliated Hospital of Zunyi Medical University, Zunyi, 550000, Guizhou, China.

Stellate ganglion block (SGB) has been applied in clinic for almost a century as a therapeutic procedure to alleviate pain-related syndromes and vascular deficits in the upper extremities. A great number of causative side effects and complications due to technological insufficiency and anatomical variations called for the popularity of ultrasound-guided SGB which has made tremendous contribution for clinical diagnosis and therapy, primarily in postoperative pain and cardiac and vascular disorders. This work was aimed at systematically summarizing the current clinical application of ultrasound-guided SGB and putting forward the potential prospective application in future. By searching ultrasound-guided SGB-related works on PubMed database, we mainly elucidated the analgesic effect of preoperative SGB in patients undergoing surgical procedures and substantial reduction in patients with ventricular arrhythmias. The volume of local anesthetics used in ultrasound-guided SGB has been diminished in the recent few years' investigations and successful operation of ultrasound-guided SGB could be achieved with minimal safe volume of local anesthetics. This invasive and safe procedure shows vast potential for future development in clinical treatment for autonomic nervous system and autoimmune disorders. We also put forward hypothesis that ultrasound-guided SGB could be applied combined with controlled hypotension to reduce the intraoperative complications in orthopedic surgery such as insufficiency of cerebral blood flow and reflexive tachycardia. Thus, it is of vital essence to improve the professional skills of physicians for the high rate of success and explore more effective measures which could enhance therapeutic effects when combined with ultrasound-guided SGB in alleviating misery of patients.
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http://dx.doi.org/10.1007/s10072-021-05300-4DOI Listing
May 2021

Illness uncertainty among patients with COVID-19 in the Mobile Cabin Hospital.

Nurs Open 2021 May 17. Epub 2021 May 17.

Heart Center & Beijing Key Laboratory of Hypertension, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

Aims: To investigate the status and influencing factors of illness uncertainty among patients with coronavirus disease 2019 (COVID-19) in the mobile cabin hospital.

Design: A cross-sectional study.

Methods: 114 patients with COVID-19 admitted to a mobile cabin hospital in Wuhan, Hubei Province, in February 2020 were enrolled by a convenience sampling method. The Chinese version of the Mishel Illness Uncertainty Scale (MUIS) was used to assess patients' degree of illness uncertainty, and multiple regression analysis was used to explore the influencing factors.

Results: The average total score of MUIS (Chinese version) was 52.22 ± 12.51, indicating a moderate level of illness uncertainty. The dimension unpredictability turned out to have the highest mean score: 2.88 ± 0.90. The multiple stepwise regression analysis showed that female (t = 2.462, p = .015), monthly family income not less than RMB 10,000 (t = -2.095, p = .039), and disease duration of 28 days or more (t = 2.249, p = .027) were independent influencing factors of illness uncertainty.

Conclusion: Patients with COVID-19 are at a moderate level of illness uncertainty. Medical staffs should pay more attention to female patients, patients with lower monthly family income, patients with the prolonged disease, and take targeted interventions to help them reduce illness uncertainty.

Impact: Facing the brand new and unknown infectious disease, patients confirmed of COVID-19 suffer from immense physical and psychological stress, where illness uncertainty is a major stressor that troubles patients. The present study surveys illness uncertainty among patients with COVID-19 in the mobile cabin hospital with results revealing a moderate level. Study results will benefit nurses in any setting where care for patients with COVID-19 is provided, public policymakers and future researchers.
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http://dx.doi.org/10.1002/nop2.924DOI Listing
May 2021

Use of Circulating Tumor DNA for the Clinical Management of Metastatic Castration-Resistant Prostate Cancer: A Multicenter, Real-World Study.

J Natl Compr Canc Netw 2021 May 14:1-10. Epub 2021 May 14.

1Department of Urology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai.

Background: This study aimed to describe the aberrations of DNA damage repair genes and other important driving genes in Chinese patients with metastatic castration-resistant prostate cancer (mCRPC) using circulating tumor (ctDNA) sequencing and to evaluate the associations between the clinical outcomes of multiple therapies and key genomic alterations in mCRPC, especially DNA damage repair genes.

Patients And Methods: A total of 292 Chinese patients with mCRPC enrolled from 8 centers. Multigene targeted sequencing was performed on 306 ctDNA samples and 23 matched tumor biopsies. The frequency of genomic alterations were compared with the Stand Up to Cancer-Prostate Cancer Foundation (SU2C-PCF) cohort. The Kaplan-Meier method was used to evaluate progression-free survival (PFS) following standard systemic treatments for mCRPC. Cox regression analyses were performed to determine prognostic factors associated with PFS resulting from treatments for mCRPC.

Results: In total, 33 of 36 (91.7%) mutations were found consistently between ctDNA and paired biopsy samples. The most common recurrent genomic alterations were found in AR (34.6%), TP53 (19.5%), CDK12 (15.4%), BRCA2 (13%), and RB1 (5.8%). The frequency of CDK12 alterations (15.4%) in our cohort was significantly higher than that in Western populations (5%-7%). AR amplification and TP53 and/or RB1 alterations were associated with resistance to abiraterone or docetaxel. Patients with a CDK12 defect showed rapid disease progression after abiraterone treatment. However, the clinical outcome after docetaxel treatment was similar between patients with and without CDK12 defects. In multivariate Cox regression analysis, a CDK12 defect was significantly associated with inferior PFS after abiraterone treatment. Patients with a BRCA2 defect showed marked response to both PARP inhibitors and platinum-based chemotherapy.

Conclusions: Our study explored the genomic landscape of Chinese patients with mCRPC at different treatment stages using minimally invasive methods and evaluated the clinical implications of the driver genomic alterations on patients' response to the most widely used therapies for mCRPC. We observed a significantly higher alteration frequency of CDK12 in our cohort compared with the SU2C-PCF cohort.
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http://dx.doi.org/10.6004/jnccn.2020.7663DOI Listing
May 2021

Two factors affecting the success rate of the second non-invasive prenatal screening after initial no-call result: experience from a single tertiary center in China.

Chin Med J (Engl) 2021 May 13. Epub 2021 May 13.

Department of Prenatal Diagnosis, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu 210004, China Center of Genetic Medicine, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu 210004, China.

Background: One inevitable shortcoming of non-invasive prenatal screening (NIPS)/cell-free DNA (cfDNA) sequencing is the uninterpretable ("no-call") result, which is mainly caused by an insufficient fetal fraction. This study was performed to investigate the factors associated with a successful second NIPS in these cases and determine the optimal management for women with initial no-call results.

Methods: We retrospectively analyzed the data of women who underwent NIPS with initial no-call results due to an insufficient fetal fraction from 2017 to 2019 in our center. We compared these women's maternal and pregnancy information with the data of women who had attained a successful second NIPS result and women who had received no-call results for a second time.

Results: Among the 33,684 women who underwent NIPS, 137 with a no-call result underwent a retest. Comparison between the 87 (63.50%) women with a successful retest and the other 50 (36.50%) women showed a significant difference in both the initial fetal fraction and maternal body mass index (BMI), whereas the other factors showed no significant differences. In addition, with an initial fetal fraction of < 2.00%, the retest success rate was very limited.

Conclusions: We identified two major factors associated with a successful NIPS retest: the initial fetal fraction and the maternal BMI. These findings suggest the need for specialized management for this subset of women and would be instructional for the counseling for these women.
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http://dx.doi.org/10.1097/CM9.0000000000001531DOI Listing
May 2021

Trust-Tech Source-Point Method for Systematically Computing Multiple Local Optimal Solutions: Theory and Method.

IEEE Trans Cybern 2021 May 13;PP. Epub 2021 May 13.

In this article, a Trust-Tech source-point method is proposed to systematically compute multiple local optimal solutions (LOSs) for continuous unconstrained nonlinear optimization problems. This proposed method consists of four stages. Stage I finds one LOS (in which existing effective optimizers can be applied), stage II is the stage of escaping an LOS while stage III is the stage for entering the stability region (SR) of another stable equilibrium point (SEP) (i.e., another LOS). Stage IV computes other SEPs (i.e., LOSs) in corresponding SRs. A theoretical foundation for both stages II and III is developed, and these theoretical results are quite general on their own. The proposed method is numerically evaluated to compute multiple LOSs. For instance, a total of 5085 LOSs have been computed by the proposed Trust-Tech source point method on a 50-D test function. In addition, the proposed method can find the global optimal solutions of several test functions with 50 dimensions and 100 dimensions.
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http://dx.doi.org/10.1109/TCYB.2021.3071462DOI Listing
May 2021

Is there any association between asthma and Kawasaki disease? A systematic review and meta-analysis.

Pediatr Pulmonol 2021 May 13. Epub 2021 May 13.

Department of Respiratory, Shandong Provincial Qianfoshan Hospital, The First Affiliated Hospital of Shandong First Medical University, Shandong Institute of Respiratory Diseases, Shandong University, Jinan, China.

Objective: Shared immune factors are involved in the pathogenesis of asthma and Kawasaki disease (KD). Consequently, several observational studies have explored an association between KD and asthma. This systematic review and meta-analysis aimed to explore the association between KD and asthma.

Methods: PubMed and Embase databases were searched for cross-sectional, case-control and cohort studies that investigated the association between KD and asthma. The first author, publication year, study type, sample size, and participant demographics were retrieved. The association between KD and asthma was expressed by the odds ratio (OR) and 95% confidence interval (CI).

Results: Eleven studies were eligible in the systematic review. The crude ORs (cORs) were extracted from 11 studies, and seven of them also provided adjusted ORs (aORs). The cORs between KD and asthma was 1.53 (95% CI, 1.29-1.81). After adjustment for some confounding factors (age, sex, region), asthma also had an association with KD (aOR, 1.08; 95% CI, 1.00-1.17). The subgroup analyses showed the study design and study location to influence between-study heterogeneity. Analyses of aOR values revealed that asthma onset before KD onset does not appear to increase KD prevalence, whereas people suffering from KD could increase the prevalence of developing asthma.

Conclusion: Our meta-analysis suggested a possible association between preceding KD and asthma onset, but care must be taken when interpreting this result. Prospective cohort studies are needed to assess a causal relationship between these two diseases.
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http://dx.doi.org/10.1002/ppul.25443DOI Listing
May 2021

Overexpression of hsa_circ_0001445 reverses oxLDL‑induced inhibition of HUVEC proliferation via SRSF1.

Mol Med Rep 2021 Jul 13;24(1). Epub 2021 May 13.

Department of Cardiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China.

Atherosclerosis is a primary cause of multiple types of cardiovascular disease, including myocardial infarction. In addition, injury of human umbilical vein endothelial cells (HUVECs) can lead to the development of atherosclerosis. Circular (circ)RNAs participate in atherosclerosis. It has previously been shown that circRNA cSMARCA5 (hsa_circ_0001445) expression is downregulated in atherosclerosis. However, the effects of hsa_circ_0001445 on the proliferation of HUVECs remain unclear. In order to mimic atherosclerosis , HUVECs were treated with oxidized low‑density lipoprotein (oxLDL). The expression levels of specific genes and proteins were detected in HUVECs by reverse transcription‑quantitative PCR and western blot analysis, respectively. Cell proliferation was assessed by Cell Counting Kit‑8 and 5‑Ethynyl‑2'‑deoxyuridine staining. Cell apoptosis and 5,5',6,6'‑Tetrachloro‑1,1',3,3'‑tetraethyl‑imidacarbocyanine staining were examined by flow cytometry. In addition, the association between hsa_circ_0001445 and serine/arginine‑rich splicing factor 1 (SRSF1) was investigated by RNA pull‑down assay. hsa_circ_0001445 expression was downregulated in oxLDL‑treated HUVECs. Moreover, oxLDL‑induced inhibition of HUVEC proliferation was significantly reversed by overexpression of hsa_circ_0001445. oxLDL notably inhibited tube formation and mitochondrial membrane potential in HUVECs, while these effects were markedly reversed by hsa_circ_0001445 overexpression. Furthermore, overexpression of hsa_circ_0001445 reversed oxLDL‑induced activation of β‑catenin by binding to SRSF1. Collectively, these data demonstrated that overexpression of hsa_circ_0001445 reversed oxLDL‑induced inhibition of HUVEC proliferation via activation of the SRSF1/β‑catenin axis. These findings may provide novel targets for the treatment of atherosclerosis.
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http://dx.doi.org/10.3892/mmr.2021.12146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134882PMC
July 2021

A curcumin analog GL63 inhibits the malignant behaviors of hepatocellular carcinoma by inactivating the JAK2/STAT3 signaling pathway via the circular RNA zinc finger protein 83/microRNA-324-5p/cyclin-dependent kinase 16 axis.

J Gastroenterol Hepatol 2021 May 12. Epub 2021 May 12.

Department of Burn and Plastic Surgery, The First Hospital, Hebei Medical University, Shijiazhuang, China.

Background And Aim: (1E,4E)-1,5-bis(2-bromophenyl) penta-1,4-dien-3-one (GL63) is a curcumin analog that can protect against carcinogenesis in hepatocellular carcinoma (HCC). The aim of this study was to explore the molecular mechanism of GL63 in HCC.

Methods: Cell viability was examined by cell counting kit-8 (CCK-8) assay. Circular RNA zinc finger protein 83 (circZNF83), microRNA-324-5p (miR-324-5p), and cyclin-dependent kinase 16 (CDK16) levels were measured via the quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation was assessed using colony formation assay. Flow cytometry was performed for detecting cell cycle and apoptosis. Protein analysis was conducted by western blot. Cell migration and invasion were determined using transwell assay. Target relation was analyzed using dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. The function of GL63 in vivo was researched by xenograft model in mice.

Results: GL63 inhibited the circZNF83 expression in HCC cells. CircZNF83 overexpression attenuated the inhibitory effects of GL63 on HCC cell growth, cell cycle progression, migration, and invasion but the promoting effect on cell apoptosis. CircZNF83 served as a sponge of miR-324-5p and circZNF83/miR-324-5p axis was involved in the functional regulation of GL63 in HCC progression. Moreover, CDK16 was a downstream target of miR-324-5p and circZNF83 could regulate the CDK16 expression by sponging miR-324-5p. The anti-tumor function of GL63 was also related to the miR-324-5p/CDK16 axis. In addition, GL63 inactivated the JAK2/STAT3 pathway via downregulating circZNF83 to mediate the miR-324-5p/CDK16 axis. GL63 also repressed tumor growth in vivo through the circZNF83/miR-324-5p/CDK16-mediated JAK2/STAT3 signal inhibition.

Conclusion: This study suggested GL63 impeded the HCC development by blocking the JAK2/STAT3 signaling pathway via mediating the circZNF83/miR-324-5p/CDK16 axis.
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http://dx.doi.org/10.1111/jgh.15545DOI Listing
May 2021

Mathematical model and characteristics analysis of crossed-axis helical gear drive with small angle based on curve contact element.

Sci Prog 2021 Apr-Jun;104(2):368504211016202

The State Key Laboratory of Mechanical Transmission, Chongqing University, Chongqing, China.

To improve load capacity and transmission characteristics of crossed-axis helical gear drive, a generation approach of the gear pair with small-angle based on the curve contact element is proposed. Contact principle based on spatial curve meshing relationships is introduced and geometric models of tooth profiles are developed according to a pair of mated conjugate curves. Furthermore, a mathematical model of crossed-axis helical gear drive with small-angle is established. Numerical examples are illustrated for this research using the 10° shaft angle, and the computerized simulation is also developed based on the solid models. According to gear geometry and finite element method, general characteristics including undercutting conditions, sliding ratios and contact stress for tooth profiles are analyzed. Comparisons with crossed-axis involute gears are also carried out. Finally, the gear prototype is processed using the gear milling method and a basic performance test is conducted. Analysis results show that the new gear pair has well contact characteristics. Further studies on the dynamic analysis and precision manufacturing method will be carried out.
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http://dx.doi.org/10.1177/00368504211016202DOI Listing
May 2021

The seborrheic dermatitis-distributed dermatomyositis: A facial cutaneous lesions sign associated with positive anti-MDA5 antibody.

J Cosmet Dermatol 2021 May 8. Epub 2021 May 8.

Department of Allergy, Zibo Central Hospital, Zibo, China.

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http://dx.doi.org/10.1111/jocd.14204DOI Listing
May 2021

Activation of TREM-1 induces endoplasmic reticulum stress through IRE-1α/XBP-1s pathway in murine macrophages.

Mol Immunol 2021 Jul 3;135:294-303. Epub 2021 May 3.

Department of Anesthesiology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, 563000, China. Electronic address:

Increasing evidence suggests that endoplasmic reticulum (ER) stress activates several pro-inflammatory signaling pathways in many diseases, including acute lung injury (ALI). We have reported that blocking triggering receptor expressed on myeloid cells 1 (TREM-1) protects against ALI by suppressing pulmonary inflammation in mice with ALI induced by lipopolysaccharides (LPS). However, the molecular mechanism underlying the TREM-1-induced pro-inflammatory microenvironment in macrophages remains unclearly. Herein, we aimed to determine whether TREM-1 regulates the inflammatory responses induced by LPS associated with ER stress activation. We found that the activation of TREM-1 by a monoclonal agonist antibody (anti-TREM-1) increased the mRNA and protein levels of IL-1β, TNF-α, and IL-6 in primary macrophages. Treatment of the anti-TREM-1 antibody increased the expression of ER stress markers (ATF6, PERK, IRE-1α, and XBP-1s) in primary macrophages. While pretreatment with 4-PBA, an inhibitor of ER stress, significantly inhibited the expression of ER stress markers and pro-inflammatory cytokines and reduced LDH release. Furthermore, inhibiting the activity of the IRE-1α/XBP-1s pathway by STF-083010 significantly mitigated the increased levels of IL-1β, TNF-α, and IL-6 in macrophages treated by the anti-TREM-1 antibody. XBP-1 silencing attenuated pro-inflammatory microenvironment evoked by activation of TREM-1. Besides, we found that blockade of TREM-1 with LR12 ameliorated ER stress induced by LPS in vitro and in vivo. In conclusion, we conclude that TREM-1 activation induces ER stress through the IRE-1α/XBP-1s pathway in macrophages, contributing to the pro-inflammatory microenvironment.
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http://dx.doi.org/10.1016/j.molimm.2021.04.023DOI Listing
July 2021

Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms.

Nat Commun 2021 05 5;12(1):2547. Epub 2021 May 5.

Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China.

Receptors and their ligands are important therapeutic targets for about one third of marketed drugs. Here, we describe an epitope-guided approach for selection of antibodies that modulate cellular signaling of targeted receptors. We chose CXC chemokine receptor 2 (CXCR2) in the G-protein coupled receptor superfamily as receptor and a CXCR2 N-terminal peptide for antibody selection. We obtain a highly selective, tight-binding antibody from a 10-member antibody library using combinatorial enrichment. Structural and Hydrogen-Deuterium-Exchange mass spectrometry analyses demonstrate antibody interaction with an N-terminal region of CXCR2 that is part of the IL-8 epitope. The antibody strongly inhibits IL-8-induced and CXCR2-mediated neutrophil chemotaxis in vitro and alleviates hCXCR2-dependent experimental autoimmune encephalomyelitis symptoms in mice. As inappropriate neutrophil migration accompanies many diseases including inflammatory bowel disease, glomerulonephritis, allergic asthma, chronic obstructive pulmonary disease, and cancer, this antibody has potential for development as a therapeutic agent, akin to anti-TNF antibodies. However, an important difference here is that the antibody targets the chemokine receptor and competes with natural ligand, rather than targeting the ligand itself.
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http://dx.doi.org/10.1038/s41467-021-22810-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100106PMC
May 2021

Multicenter clinical evaluation of a piezoresistive-MEMS-sensor rapid-exchange pressure microcatheter system for fractional flow reserve measurement.

Catheter Cardiovasc Interv 2021 May 5. Epub 2021 May 5.

Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai, China.

Objectives: This multicenter, prospective clinical study investigates whether the microelectromechanical-systems-(MEMS)-sensor pressure microcatheter (MEMS-PMC) is comparable to a conventional pressure wire in fractional flow reserve (FFR) measurement.

Background: As a conventional tool for FFR measurement, pressure wires (PWs) still have some limitations such as suboptimal handling characteristics and unable to maintain the wire position during pullback assessment. Recently, a MEMS-PMC compatible with any 0.014″ guidewire is developed. Compared with the existing optical-sensor PMC, this MEMS-PMC has smaller profiles at both the lesion crossing and sensor packaging areas.

Methods: Two hundred and forty-two patients with visually 30-70% coronary stenosis were enrolled at four centers. FFR was measured first with the MEMS-PMC, and then with the PW. The primary endpoint was the Bland-Altman mean bias between the MEMS-PMC and PW FFR.

Results: From the 224-patient per-protocol data, quantitative coronary angiography showed 17.9% and 55.9% vessels had diameter < 2.5 mm and stenosis >50%, respectively. The two systems' mean bias was -0.01 with [-0.08, 0.06] 95% limits-of-agreement. Using PW FFR≤0.80 as cutoff, the MEMS-PMC per-vessel diagnostic accuracy was 93.4% [95% confidence interval: 89.4-96.3%]. The MEMS-PMC's success rate was similar to that of PW (97.5 vs. 96.3%, p = .43) with no serious adverse event, and its clinically-significant (>0.03) drift rate was 43% less (9.5 vs. 16.7%, p = .014).

Conclusions: Our study showed the MEMS-PMC is safe to use and has a minimal bias equal to the resolution of current FFR systems. Given the MEMS-PMC's high measurement accuracy and rapid-exchange nature, it may become an attractive new tool facilitating routine coronary physiology assessment.
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http://dx.doi.org/10.1002/ccd.29678DOI Listing
May 2021

Correcting motion artifacts in coronary computed tomography angiography images using a dual-zone cycle generative adversarial network.

J Xray Sci Technol 2021 Apr 26. Epub 2021 Apr 26.

Lauterbur Research Center for Biomedical Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

Background: Coronary computed tomography angiography (CCTA) is a noninvasive imaging modality to detect and diagnose coronary artery disease. Due to the limitations of equipment and the patient's physiological condition, some CCTA images collected by 64-slice spiral computed tomography (CT) have motion artifacts in the right coronary artery, left circumflex coronary artery and other positions.

Objective: To perform coronary artery motion artifact correction on clinical CCTA images collected by Siemens 64-slice spiral CT and evaluate the artifact correction method.

Methods: We propose a novel method based on the generative adversarial network (GAN) to correct artifacts of CCTA clinical images. We use CCTA clinical images collected by 64-slice spiral CT as the original dataset. Pairs of regions of interest (ROIs) cropped from original dataset or images with and without motion artifacts are used to train the dual-zone GAN. When predicting the CCTA images, the network inputs only the clinical images with motion artifacts.

Results: Experiments show that this network effectively corrects CCTA motion artifacts. Regardless of ROIs or images, the peak signal to noise ratio (PSNR), structural similarity (SSIM), mean square error (MSE) and mean absolute error (MAE) of the generated images are greatly improved compared to those of the input data. In addition, based on scores from physicians, the average score for the coronary artery artifact correction of the output images is higher.

Conclusions: This study demonstrates that the dual-zone GAN has the excellent ability to correct motion artifacts in the coronary arteries and maintain the overall characteristics of CCTA clinical images.
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http://dx.doi.org/10.3233/XST-210841DOI Listing
April 2021

Development and Validation of a Sensitive, Specific and Reproducible UPLC-MS/MS Method for the Quantification of OJT007, A Novel Anti-Leishmanial Agent: Application to a Pharmacokinetic Study.

Int J Environ Res Public Health 2021 04 27;18(9). Epub 2021 Apr 27.

Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Texas Southern University, Houston, TX 77004, USA.

OJT007 is a methionine aminopeptidase 1 (MetAP1) inhibitor with potent anti-proliferative effects against . In order to study its pharmacokinetics as a part of the drug development process, a sensitive, specific, and reproducible ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated. Voriconazole was used as the internal standard to generate standard curves ranging from 5 to 1000 ng/mL. The separation was achieved using a UPLC system equipped with an Acquity UPLC BEH C column (2.1 × 50 mm, 1.7 μm) with 0.1% formic acid in acetonitrile and 0.1% formic acid in water as the mobile phase under gradient elution at a flow rate of 0.4 mL/min. The mass analysis was performed with a 4000 QTRAP mass spectrometer using multiple-ion reaction monitoring (MRM) in the positive mode, with the transition of / 325 → / 205 for OJT007 and / 350 → / 101 for voriconazole. The intra- and inter-day precision and accuracy were within ±15%. The mean extraction recovery and the matrix effect were 95.1% and 7.96%, respectively, suggesting no significant matrix interfering with the quantification of the drug in rat plasma. This study was successfully used for the pharmacokinetic evaluation of OJT007 using the rat as an animal model.
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http://dx.doi.org/10.3390/ijerph18094624DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123827PMC
April 2021

Pharmacokinetic Model Analysis of Supralingual, Oral and Intravenous Deliveries of Mycophenolic Acid.

Pharmaceutics 2021 Apr 17;13(4). Epub 2021 Apr 17.

Department of Pharmaceutical Science, College of Pharmacy and Health Sciences, Texas Southern University, Houston, TX 77004, USA.

Mycophenolic acid (MPA) is commonly used for organ rejection prophylaxis via oral administration in the clinic. Recent studies have shown that MPA also has anticancer activities. To explore new therapeutic options for oral precancerous/cancerous lesions, MPA was designed to release topically on the dorsal tongue surface via a mucoadhesive patch. The objective of this study was to establish the pharmacokinetic (PK) and tongue tissue distribution of mucoadhesive MPA patch formulation after supralingual administration in rats and also compare the PK differences between oral, intravenous, and supralingual administration of MPA. Blood samples were collected from Sprague Dawley rats before and after a single intravenous bolus injection, a single oral dose, or a mucoadhesive patch administration on the dorsal tongue surface for 4 h, all with a dose of 0.5 mg/kg of MPA. Plots of MPA plasma concentration versus time were obtained. As multiple peaks were found in all three curves, the enterohepatic recycling (EHR) model in the Phoenix software was adapted to describe their PK parameters with an individual PK analysis method. The mean half-lives of intravenous and oral administrations were 10.5 h and 7.4 h, respectively. The estimated bioavailability after oral and supralingual administration was 72.4% and 7.6%, respectively. There was a 0.5 h lag-time presented after supralingual administration. The results suggest that the systemic plasma MPA concentrations were much lower in rats receiving supralingual administration compared to those receiving doses from the other two routes, and the amount of MPA accumulated in the tongue after patch application showed a sustained drug release pattern. Studies on the dynamic of drug retention in the tongue after supralingual administration showed that ~3.8% of the dose was accumulated inside of tongue right after the patch removal, ~0.11% of the dose remained after 20 h, and ~20.6% of MPA was not released from the patches 4 h after application. The data demonstrate that supralingual application of an MPA patch can deliver a high amount of drug at the site of administration with little systemic circulation exposure, hence lowering the potential gastrointestinal side effects associated with oral administration. Thus, supralingual administration is a potential alternative route for treating oral lesions.
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http://dx.doi.org/10.3390/pharmaceutics13040574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072905PMC
April 2021

Using Polychlorinated Naphthalene Concentrations in the Soil from a Southeast China E-Waste Recycling Area in a Novel Screening-Level Multipathway Human Cancer Risk Assessment.

Environ Sci Technol 2021 May 26;55(10):6773-6782. Epub 2021 Apr 26.

National Research Center for Environmental Analysis and Measurement, Beijing 100029, China.

Polychlorinated naphthalene (PCN) concentrations in the soil at an e-waste recycling area in Guiyu, China, were measured and the associated human cancer risk due to e-waste-related exposures was investigated. We quantified PCNs in the agricultural soil and used these concentrations with predictive equations to calculate theoretical concentrations in outdoor air. We then calculated theoretical concentrations in indoor air using an attenuation factor and in the local diet using previously published models for contaminant uptake in plants and fruits. Potential human cancer risks of PCNs were assessed for multiple exposure pathways, including soil ingestion, inhalation, dermal contact, and dietary ingestion. Our calculations indicated that local residents had a high cancer risk from exposure to PCNs and that the diet was the primary pathway of PCN exposure, followed by dermal contact as the secondary pathway. We next repeated the risk assessment using concentrations for other carcinogenic contaminants reported in the literature at the same site. We found that polychlorinated dibenzodioxins and dibenzofurans (PCDD/Fs) and PCNs caused the highest potential cancer risks to the residents, followed by polychlorinated biphenyls (PCBs). The relative importance of different exposure pathways depended on the physicochemical properties of specific chemicals.
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http://dx.doi.org/10.1021/acs.est.1c00128DOI Listing
May 2021

[Expert Consensus for Thermal Ablation of Pulmonary Subsolid Nodules (2021 Edition)].

Zhongguo Fei Ai Za Zhi 2021 May 26;24(5):305-322. Epub 2021 Apr 26.

Department of Oncology, Tengzhou Central People's Hospital, Tengzhou 277500, China.

"The Expert Group on Tumor Ablation Therapy of Chinese Medical Doctor Association, The Tumor Ablation Committee of Chinese College of Interventionalists, The Society of Tumor Ablation Therapy of Chinese Anti-Cancer Association and The Ablation Expert Committee of the Chinese Society of Clinical Oncology" have organized multidisciplinary experts to formulate the consensus for thermal ablation of pulmonary subsolid nodules or ground-glass nodule (GGN). The expert consensus reviews current literatures and provides clinical practices for thermal ablation of GGN. The main contents include: (1) clinical evaluation of GGN, (2) procedures, indications, contraindications, outcomes evaluation and related complications of thermal ablation for GGN and (3) future development directions.
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http://dx.doi.org/10.3779/j.issn.1009-3419.2021.101.14DOI Listing
May 2021

Enhanced performance of sulfamethoxazole degradation using Achromobacter sp. JL9 with in-situ generated biogenic manganese oxides.

Bioresour Technol 2021 Aug 1;333:125089. Epub 2021 Apr 1.

School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China.

Little information is known about the relationships of in-situ generated BioMnOx and sulfamethoxazole (SMX) degradation. In this study, a novel efficient bioremediation technology was presented for simultaneous remove the nitrogen-N, SMX, and Mn(II) from water. Mn(II) can be completely oxidized with a oxidized rate of 0.071 mg/(L·h), the SMX and nitrogen-N removal ratios were 97.43% and 85.61%, respectively. The Ratkowsky kinetic models were established for described the SMX degradation influence by temperature. Furthermore, the microbial degradation, Mn(III) trapping, and intermediates identified experiments were used to explore the mechanisms of SMX and nitrogen-N removal. These results indicated that microbial activity play a decisive role in SMX and nitrogen-N removal, and the catalytic character of sediment could enhanced the SMX degradation. Furthermore, proposed the possible SMX degradation pathway based on the intermediates and microbial metabolism theory, the environmental toxicity of SMX and each intermediates were calculated via ECOSAR program.
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http://dx.doi.org/10.1016/j.biortech.2021.125089DOI Listing
August 2021

Photoelectrochemical detection of human epidermal growth factor receptor 2 (HER2) based on CoO-ascorbic acid oxidase as multiple signal amplifier.

Mikrochim Acta 2021 Apr 20;188(5):166. Epub 2021 Apr 20.

Department of Dermatology, Xiangya Hospital, Central South University, Changsha, 410008, China.

A sensitive photoelectrochemical (PEC) sensor based on hexagonal carbon nitride tubes (HCNT) as photoactive material was prepared for the detection of human epidermal growth factor receptor 2 (HER2). Magnetic FeO nanospheres (MNs) modified with anti-HER2 antibodies were employed for highly efficient capture of HER2 from serum sample, and CoO nanoparticles (CoO NPs) modified with ascorbic acid oxidase (AAO) as well as HER2 aptamer were used for signal amplification. When the aptamer-CoO-AAO probe was captured onto the electrode surface through the specific binding of the aptamer with HER2, the photocurrent intensity decreased. This was because CoO NPs competed with HCNT for consumption of the excitation energy. As a consequence AAO catalyzed the oxidation of the electron donor (AA), and the aptamer-CoO-AAO probe increased the steric hindrance at the electrode surface, leading to significant photocurrent intensity decrease, thus realizing multiple signal amplification. Based on this signal amplification strategy, at 0 V (vs Ag/AgCl), the PEC sensor shows a wide linear response ranging from 1 pg mL to 1 ng mL with a low detection limit of 0.026 pg mL for HER2. Importantly, the prepared PEC sensor was applied for detection of HER2 in human serum samples with recoveries between 98.8 and 101%. Sensitive photoelectrochemical sensor based on CoO nanoparticles modified with ascorbic acid oxidase for signal amplification is reported.
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http://dx.doi.org/10.1007/s00604-021-04829-7DOI Listing
April 2021