Publications by authors named "Lian Zheng"

60 Publications

Laparoscopic percutaneous extraperitoneal closure with peritoneum reinforcement repair for pediatric inguinal hernia: a single-center experience with over 2,000 patients.

Transl Pediatr 2021 May;10(5):1317-1323

Department of Pediatric Surgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, China.

Background: Inguinal hernia is one of the common diseases in infants and children that requires operative treatment. Laparoscopic inguinal hernia repair in children has become an alternative to the open procedure. Laparoscopic percutaneous extraperitoneal closure with peritoneum reinforcement (LPECPR) is a safe and effective approach for pediatric inguinal hernia, and has a lower recurrence. This is a retrospective study to present our experience with children who underwent LPECPR.

Methods: A total of 2,018 patients with inguinal hernia who underwent LPECPR in our hospital from July, 2011 to December, 2020 were reviewed. The surgical technique is modified on the basis of laparoscopic percutaneous extraperitoneal closure (LPEC) to close extraperitoneally by circuit suturing twice around the internal inguinal ring.

Results: All cases were completed LPECPR without conversion. There were no intraoperative complications. A total of 2,018 patients' laparoscopic procedures were achieved. The mean operative time was 14 and 20 min for unilateral and bilateral operations, respectively. Follow-up to date is 13.4 months (6-36 months), there were no postoperative complications, such as knot reactions, hydrocele formation, testicular atrophy or pain, except 3 recurrences (3/2,018, 0.15%).

Conclusions: This modified LPECPR technique can acquire lower recurrence rate for repair pediatric inguinal hernia. The midterm safety and efficacy of LPECPR are proven and it can be a routine procedure.
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http://dx.doi.org/10.21037/tp-21-25DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192996PMC
May 2021

Risk factors and predictive model for abdominal wound dehiscence in neonates: a retrospective cohort study.

Ann Med 2021 12;53(1):900-907

Department of Pediatric Surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou, China.

Background: Abdominal wound dehiscence (AWD) is a major complication of abdominal surgery, and neonates are a group with a high risk of AWD, which has serious consequences or can even result in death. The purpose of this study is to explore the risk factors for neonatal AWD and construct a predictive model.

Methods: The clinical data of 453 cases that underwent neonatal laparotomy from June 2009 to June 2020 were retrospectively analyzed, among which 27 cases of AWD were identified. Nine factors, including gender, age at admission, weight at admission, preterm delivery, level of preoperative anaemia, hypoalbuminemia, operation time, incision length, and incision type, were analyzed to explore their correlation with neonatal AWD.

Results: The incidence of neonatal AWD was 6.0% (27/453), among which partial wound dehiscence accounted for 4.9% (22/453) and complete wound dehiscence accounted for 1.1% (5/453). Hypoproteinemia and incision type were the independent risk factors for neonatal AWD, and weight at admission was a protective factor for AWD in the multivariate models. All these factors were incorporated to construct a nomogram, and a calibration curve was plotted. The result indicated that the actual risk was close to the predicted risk when the predicted risk rate was greater than about 35%.

Conclusions: Neonatal AWD is closely related to hypoproteinemia and incision contamination. Our predictive model showed the potential to provide an individualized risk estimate of AWD for neonatal patients undergoing abdominal surgery.Key messagesNeonatal abdominal wound dehiscence (AWD) has a serious consequence and the incidence of neonatal AWD was about 6.0% and the complete AWD morbidity is 1.1%.Hypoproteinemia and incision type were the independent risk factors for neonatal AWD.Our predictive model showed the potential to provide an individualized risk estimate of AWD for neonatal patients undergoing abdominal surgery.
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http://dx.doi.org/10.1080/07853890.2021.1938661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204998PMC
December 2021

Thermophoretic collection of virus-laden (SARS-CoV-2) aerosols.

Biomicrofluidics 2021 Mar 12;15(2):024101. Epub 2021 Mar 12.

HiFiBio (Hangzhou) Co., Ltd., Hangzhou 311215, China.

Detecting the existence of SARS-CoV-2 in the indoor atmosphere is a practical solution to track the prevalence and prevent the spread of the virus. In this work, a thermophoretic approach is presented to collect the novel coronavirus-laden aerosols from the air and accumulate to high concentrations adequate for the sensitivity of viral RNA detection. Among the factors, the density and particle size have negligible effects on particle trajectory, while the vertical coordinates of particles increase with the rise in heating source temperature. When the heating temperature is higher than , all of the particles exit the channel from one outlet; thus, the collecting and accumulating of virus-laden aerosols can be realized. This study provides a potential approach to accelerate the detection of SARS-CoV-2 and avoid a false negative in the following RNA test.
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http://dx.doi.org/10.1063/5.0039247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955857PMC
March 2021

Exosome-derived long non-coding RNA ZFAS1 controls cardiac fibrosis in chronic kidney disease.

Aging (Albany NY) 2021 Feb 26;13. Epub 2021 Feb 26.

Department of Nephrology, Cangzhou Central Hospital of Hebei Province, Cangzhou 061000, Hebei Province, China.

The incidence of chronic kidney disease (CKD) complicated with cardiovascular disease (CVD) is increasing day by day. CVD complication is a significant risk factor for the progression and poor prognosis of CKD. However, in terms of clinical diagnosis, treatment, and pathophysiological mechanism, there are still many things that are not clear about CKD combined with CVD. In this article, we observed the abnormal expression of long non-coding RNA ZFAS1 (lncRNA ZFAS1) in the heart and renal tissues of CKD mice, which may a target for nephropathy with CVD. We observed that exosomes containing lncRNA ZFAS1 induced fibrosis in the heart via the wnt4/β-catenin signal pathway. Luciferase assay reported that lncRNA ZFAS1 could bind with microRNA-4711-5p (miR-4711-5p), and wnt4 was a target of miR-4711-5p. In conclusion, lncRNA ZFAS1 from HCM could promote cardiac fibrosis through WNT/β-catenin signal via exosome pathways, which provided an underlying mechanism in the cardiac fibrosis from CKD.
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http://dx.doi.org/10.18632/aging.202599DOI Listing
February 2021

Bioinformatics analysis of DNMT1 expression and its role in head and neck squamous cell carcinoma prognosis.

Sci Rep 2021 Jan 26;11(1):2267. Epub 2021 Jan 26.

Department of General Dentistry, The First Affiliated Hospital, Zhengzhou University, No. 1 Jianshe Road, Zhengzhou, 450052, Henan, China.

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common type of malignancy in the world. DNA cytosine-5-methyltransferase 1 (DNMT1) play key roles in carcinogenesis and regulation of the immune micro-environment, but the gene expression and the role of DNMT1 in HNSCC is unknown. In this study, we utilized online tools and databases for pan-cancer and HNSCC analysis of DNMT1 expression and its association with clinical cancer characteristics. We also identified genes that positively and negatively correlated with DNMT1 expression and identified eight hub genes based on protein-protein interaction (PPI) network analysis. Enrichment analyses were performed to explore the biological functions related with of DNMT1. The Tumor Immune Estimation Resource (TIMER) database was performed to explore the relationship between DNMT1 expression and immune-cell infiltration. We demonstrated that DNMT1 gene expression was upregulated in HNSCC and associated with poor prognosis. Based on analysis of the eight hub genes, we determined that DNMT1 may be involved in cell cycle, proliferation and metabolic related pathways. We also found that significant difference of B cells infiltration based on TP 53 mutation. These findings suggest that DNMT1 related epigenetic alterations have close relationship with HNSCC progression, and DNMT1 could be a novel diagnostic biomarker and a promising therapeutic target for HNSCC.
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http://dx.doi.org/10.1038/s41598-021-81971-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838186PMC
January 2021

Therapeutic targets of rosuvastatin on heart failure and associated biological mechanisms: A study of network pharmacology and experimental validation.

Eur J Pharmacol 2021 Mar 22;895:173888. Epub 2021 Jan 22.

Department of Cardiology, Peking University People's Hospital, Beijing, China; Beijing Key Laboratory of Early Prediction and Intervention of Acute Myocardial Infarction, Peking University People's Hospital, Beijing, China; Center for Cardiovascular Translational Research, Peking University People's Hospital, Beijing, China. Electronic address:

To explore the potential targets underlying the effect of rosuvastatin on heart failure (HF) by utilizing a network pharmacology approach and experiments to identify the results. PharmMapper and other databases were mined for information relevant to the prediction of rosuvastatin targets and HF-related targets. Then, the rosuvastatin-HF target gene networks were created in Cytoscape software. Eventually, the targets and enriched pathways were examined by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Furthermore, we constructed an HF animal model and used rosuvastatin to treat them, identifying the changes in heart function and related protein expression. We further used different cells to explore the mechanisms of rosuvastatin. Thirty-five intersection targets indicated the therapeutic targets linked to HF. GO analysis showed that 481 biological processes, 4 cellular components and 23 molecular functions were identified. KEGG analysis showed 13 significant treatment pathways. In animal experiments, rosuvastatin significantly improved the cardiac function of post-myocardial infarction mice and prevented the development of HF after myocardial infarction by inhibiting IL-1Β expression. Cell experiments showed that rosuvastatin could reduce the expression of IL-1B in HUVEC and THP-1 cells. The therapeutic mechanism of rosuvastatin against HF may be closely related to the inhibition of the expression of apoptosis-related proteins, inflammatory factors, and fibrosis-related genes. However, IL-1Β is one of the most important target genes.
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http://dx.doi.org/10.1016/j.ejphar.2021.173888DOI Listing
March 2021

Gene signatures of m5C regulators may predict prognoses of patients with head and neck squamous cell carcinoma.

Am J Transl Res 2020 15;12(10):6841-6852. Epub 2020 Oct 15.

Department of General Dentistry, The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052, Henan, P. R. China.

Background: Head and neck squamous cell carcinoma (HNSCC) is the fifth most common malignancy in the world. The 5-methylcytosine (m5C) plays vital roles in pathological conditions, such as cancer.

Methods: This study investigated The Cancer Genome Atlas (TCGA) database for patients with HNSCC. We characterized the mutations and copy number variations (CNVs) in m5C-regulatory genes, in addition to analyzing their mRNA expressions. Gene set enrichment analysis (GSEA) and protein-protein interaction (PPI) analyses were used to explore relevant functional annotations of m5C-regulatory genes.

Results: Alterations in m5C-regulatory genes were closely associated with patient clinicopathological characteristics. The expression of ten m5C-regulatory genes was significantly correlated with CNV patterns, indicating that m5C-regulatory genes have important regulatory effects. There was increased expression of m5C-regulatory genes, particularly ALYREF and NSUN5, during the tumor, node, and metastasis stages. Cox regression analysis revealed that the expression of DNMT1, TET2, and NSUN6 correlated with HNSCC prognoses. Furthermore, the expression of DNMT1 and ALYREF could effectively predict HNSCC risk in patients. In addition, the high expression levels of ALYREF correlated with mitochondrial function, and the elevated DNMT1 expression was associated with peptide cross-linking and humoral immunity. These results provide promising insight into the roles of m5C genes in tumor energy-metabolism and protein synthesis.

Conclusions: Collectively, the results indicate that m5C plays critical roles in HNSCC progression, and is also a potential HNSCC prognostic marker.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7653571PMC
October 2020

Decreased miR‑92a‑3p expression potentially mediates the pro‑angiogenic effects of oxidative stress‑activated endothelial cell‑derived exosomes by targeting tissue factor.

Int J Mol Med 2020 Nov 27;46(5):1886-1898. Epub 2020 Aug 27.

Department of Cardiology, Peking University People's Hospital, Beijing 100191, P.R. China.

Angiogenesis is an essential pathological feature of vulnerable atherosclerotic plaque. Exosome‑derived microRNAs (miRNAs or miRs) have been proven to be important regulators of angiogenesis. However, the role of exosomes, which are secreted by endothelial cells (ECs) under conditions of oxidative stress, in angiogenesis remain unclear. The present study aimed to investigate the effects and mechanism of oxidative stress‑activated endothelial‑derived exosomes in angiogenesis. Exosomes were isolated from H2O2‑stimulated human umbilical vein ECs (HUVECs; termed Exo-H2O2) by differential centrifugation and characterized by transmission electron microscopy, nanoparticle tracking analysis and western blot analysis. Exo-H2O2 enhanced HUVEC proliferation, migration and tube formation, as determined by EdU incorporation assay, scratch wound migration assay and tube formation assay, respectively. miR‑92a‑3p was identified as the predominantly downregulated miRNA in the Exo-H2O2‑treated HUVECs by small RNA sequencing, and the expression of primary miR‑92a (pri‑miR‑92a‑1) was also decreased, as shown by RT‑qPCR. Similarly, the inhibition of miR‑92a‑3p promoted angiogenesis in vitro and in vivo. miR‑92a‑3p overexpression blocked the pro‑angiogenic effects of Exo-H2O2 on target ECs. Tissue factor (TF), a molecule involved in angiogenesis, was increased in HUVECs in which miR‑92a‑3p expression was downregulated, as shown by mRNA sequencing. TF was also predicted as a target of miR‑92a‑3p by using the RNAhybrid program. The overexpression or suppression of miR‑92a‑3p modified TF expression at both the mRNA and protein level, as measured by RT‑qPCR and western blot analysis, respectively. Luciferase reporter assays suggested that miR‑92a‑3p inhibited TF expression by binding to the 3' untranslated region of TF. On the whole, the findings of the present study demonstrate that exosomes released from oxidative stress‑activated ECs stimulate angiogenesis by inhibiting miR‑92a‑3p expression in recipient ECs, and TF may be involved in the regulatory effects of miR‑92a‑3p on angiogenesis.
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http://dx.doi.org/10.3892/ijmm.2020.4713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521555PMC
November 2020

microRNA-495 alleviates endothelial cell inflammatory injury in coronary heart disease via blockade of the NF-κB pathway by targeting RALB.

J Cell Physiol 2020 Aug 18. Epub 2020 Aug 18.

The First Department of Cardiovascular Medicine, Cangzhou Central Hospital, Cangzhou, China.

Accounting for high mortality worldwide, coronary heart disease (CHD) is a severe disease characterized by abnormal intercellular communication, and microRNAs (miRNAs or miRs) have been shown to be involved in this process. This dissertation aimed to investigate the effect of miR-495 on endothelial cell inflammatory injury in CHD by targeting Ras-like small G-protein (RALB) via the nuclear factor κB (NF-κB) pathway. A high-fat-diet-induced CHD mouse model was successfully established. Endothelial cells were isolated and treated with the miR-495 mimic, miR-495 inhibitor, siRNA-RALB, or both. Next, the relationship between miR-495 and RALB was identified using the dual-luciferase reporter assay. Tumor necrosis factor α (TNF-α), interleukin-6 (IL-6), IL-10, and IL-1β levels were tested by means of enzyme-linked immunosorbent assay. Besides this, the expression of miR-495, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), RALB, NF-κB p65, and phosphorylated NF-κB p65 was determined via reverse transcription quantitative polymerase chain reaction and western blot analysis. Finally, endothelial cell proliferation, cell cycle, and apoptosis were measured. CHD mice presented with an elevated positive rate of RALB, endothelial cell apoptosis and levels of TNF-α, IL-6, IL-1β, yet a reduced IL-10 level. RALB was identified as a target of miR-495 and was downregulated by miR-495, which led to reduced expression of RALB, ICAM-1, VCAM-1, NF-κB p65, and phosphorylated NF-κB p65. miR-495 overexpression or RALB silencing decreased the levels of TNF-α, IL-6, and IL-1β, suppressed endothelial cell apoptosis and enhanced cell proliferation. In summary, miR-495 represses endothelial cell injury and inflammation through suppression of the NF-κB pathway by targeting RALB in CHD, suggesting that miR-495 may serve as a potential biomarker to monitor the progression to CHD.
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http://dx.doi.org/10.1002/jcp.29679DOI Listing
August 2020

RXFP2 as novel potential biomarker for abnormal differentiation induced by diethylstilbestrol in the gubernaculum of fetal mice.

Am J Transl Res 2020 15;12(7):3715-3727. Epub 2020 Jul 15.

Department of Radiology, The First Affiliated Hospital of Shantou University Medical College No. 57 Changping Road, Shantou 515041, Guangdong, China.

Environmental estrogens (EEs) have been correlated with abnormalities in the male urogenital system. However, the mechanism underlying the effect of these molecules remains unclear. In vitro and in vivo experiments were performed to examine the expression level and mechanism of relaxin family peptide receptor 2 (RXFP2) in the gubernaculum of fetal mice following diethylstilbestrol (DES) treatment. The in vivo results demonstrate that DES treatment increased the stillbirth rate gradually, decreased the gubernacular cone volume significantly, and disrupted the tissue structure, leading to incomplete testicular descent. In vitro experiments reveal that DES administration resulted in abnormal cellular morphology and structural disorder of gubernacular cells, which lost their original morphology in a dose-dependent manner. Moreover, DES-induced F-actin rearrangement and stress fiber formation in cultured cells. Protein quantitative analysis showed that the RXFP2 level in each experimental group was significantly lower than that of the normal group. In conclusion, DES affects the morphology and alters the gubernaculum structure, as well as the expression of RXFP2 protein. These data demonstrate that DES is toxic to gubernaculum in fetal mice, and that RXFP2 is associated with the abnormal gubernaculum morphology induced by DES. Taken together, these data suggest that RXFP2 may be a novel potential biomarker for abnormal differentiation of the gubernaculum.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407749PMC
July 2020

Efficacy and safety of catheter-based renal denervation for heart failure with reduced ejection fraction: a systematic review and meta-analysis.

Clin Auton Res 2020 12 6;30(6):521-530. Epub 2020 Aug 6.

Department of Cardiology, Peking University People's Hospital, Xizhimen South Road No. 11, Xicheng District, Beijing, 100044, China.

Purpose: To perform a comprehensive meta-analysis of all available evidence on the efficacy and safety of catheter-based renal denervation for heart failure with reduced ejection fraction.

Methods: We searched English and Chinese databases and calculated the weighted mean difference or standardized mean difference and 95% confidence intervals to estimate the efficacy and safety of renal denervation for heart failure. All relevant studies were screened and a meta-analysis was conducted using Review Manager 5.4.

Results: A total of 11 studies were identified for the meta-analysis. For the primary outcomes, the results showed that renal denervation significantly improved ejection fraction (weighted mean difference 6.42), left ventricular end-systolic diameter (weighted mean difference -3.95), left ventricular end-diastolic diameter (weighted mean difference -4.17) and left atrial diameter (weighted mean difference -4.09). For the secondary outcomes, renal denervation reduced the B-type natriuretic peptide level, heart rate, systolic blood pressure and diastolic blood pressure. However, further analysis revealed that renal denervation improved heart function but did not further reduce the heart rate and blood pressure compared with the control group.

Conclusion: Treatment with renal denervation can significantly improve heart function and structure in patients with heart failure. In addition, the level of B-type natriuretic peptide can be reduced after renal denervation treatment. Renal denervation did not further reduce heart rate and blood pressure compared with the control group. Therefore, the treatment of heart failure with renal denervation is effective and safe.
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http://dx.doi.org/10.1007/s10286-020-00716-yDOI Listing
December 2020

Long-term exposure to ephedrine leads to neurotoxicity and neurobehavioral disorders accompanied by up-regulation of CRF in prefrontal cortex and hippocampus in rhesus macaques.

Behav Brain Res 2020 09 4;393:112796. Epub 2020 Jul 4.

Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, No. 57 Changping Road, Shantou, 515041, Guangdong, China; Shantou University Medical College, No. 22 Xinling Road, Shantou, 515041, Guangdong, China; Guangdong Key Laboratory of Medical Molecular Imaging, No. 57 Changping Road, Shantou, 515041, Guangdong, China. Electronic address:

Drug addiction continues to threaten the health and welfare of people worldwide, and ephedrine abuse is a serious drug problem in many areas of the world. Ephedrine toxicity is thought to induce behavioral effects primarily through actions on the central nervous system. The corticotropin-releasing factor (CRF) system plays an important role in regulating behavioral effects induced by addictive drugs, but whether CRF is related to ephedrine toxicity remains unclear. This study seeks to examine whether there is a correlation between the CRF and chronic ephedrine neurotoxicity. To this end, we established a chronic ephedrine (0.4-1.6 mg/kg/d) exposure model in rhesus macaques, assessed its effects on body weight and behavior, examined neuronal changes in the prefrontal cortex and hippocampus, and measured the CRF expression in the prefrontal cortex and hippocampus. After 8-weeks of exposure to ephedrine, the toxic effects of ephedrine included significant weight loss and induction of behavioral changes in rhesus macaques. In particular, in the modeling group, the abnormal behavioral changes mainly manifested as irritability and behavioral sensitization. Meanwhile, the histological abnormalities included neuronal morphological changes, pyknosis and irregular shapes of neurons in the prefrontal cortex and hippocampus. In addition, the expression levels of CRF mRNA and protein were increased in the prefrontal cortex and hippocampus of ephedrine-treated animals. In summary, the finding of this study indicated that ephedrine neurotoxicity can cause neuronal damage in cerebral cortex, which in turn can result in certain neurobehavioral abnormalities, and that CRF expression in prefrontal cortex and hippocampus is elevated in response to ephedrine exposure. These observations suggested that long-term exposure to ephedrine might be causing neurotoxicity and leading to neurobehavioral disorders accompanied by up-regulation of CRF in prefrontal cortex and hippocampus.
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http://dx.doi.org/10.1016/j.bbr.2020.112796DOI Listing
September 2020

[Chondrocyte cocultures with stromal vascular fraction of adipose tissue promote cartilage regeneration in vivo].

Hua Xi Kou Qiang Yi Xue Za Zhi 2020 Jun;38(3):240-244

Dept. of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China.

Objective: This study aimed to compare the cartilage regeneration of the stromal vascular fraction (SVF) cells and adipose-derived mesenchymal stem cells (ASCs) cocultured with chondrocytes seeded on the scaffolds.

Methods: The cellular morphologies and proliferation capabilities on the scaffolds were evaluated. The scaffolds with the cocul-ture of ASCs/SVF and chondrocytes were implanted into the full thickness cartilage defective rabbit joints for 10 weeks.

Results: The cells seeded into the scaffolds showed good adhesion and proliferation. Implantation with SVF and chondrocytes revealed desirable in vitro healing outcomes.

Conclusions: The SVF cells were better than ASCs in terms of the formation of cartilage matrix in a coimplantation model. Without in vitro expansion, the SVF cells are good cell sources for cartilage repair.
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http://dx.doi.org/10.7518/hxkq.2020.03.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7296373PMC
June 2020

Cytosolic DNA sensor cGAS plays an essential pathogenetic role in pressure overload-induced heart failure.

Am J Physiol Heart Circ Physiol 2020 06 8;318(6):H1525-H1537. Epub 2020 May 8.

Department of Cardiology, Beijing Key Laboratory of Early Prediction and Intervention of Acute Myocardial Infarction, Center for Cardiovascular Translational Research, Peking University People's Hospital, Beijing, China.

Growing evidence shows that activation of inflammation in the heart provokes left ventricular (LV) remodeling and dysfunction in humans and experimental animals with heart failure (HF). Moreover, recent studies found that cyclic GMP-AMP synthase (cGAS), serving as a cytosolic DNA sensor, was essential for activating innate immunity against infection and cellular damage by initiating the STING-IRFs-type I IFN signaling cascade, which played important roles in regulating the inflammatory response. However, the pathophysiological role of cGAS in pressure overload-induced HF is unclear. Wild-type C57BL/6J mice and cGAS inhibition mice were subjected to transverse aortic constriction (TAC) to induce HF or sham operation. Inhibition of cGAS in the murine heart was performed using adeno-associated virus 9 (AAV9). Alterations of the cGAS/STING pathway were examined by qPCR and Western blotting. Cardiac remodeling was assessed by echocardiography as well as histological and molecular phenotyping. Compared with sham-operated mice, the cGAS/STING pathway was activated in LV tissues in TAC mice. Whereas TAC mice exhibited significant pathological cardiac remodeling and LV dysfunction, inhibition of cGAS improved early survival rates after TAC, preserved LV contractile function, and blunted pathological remodeling, including cardiac hypertrophy, fibrosis, and apoptosis. Furthermore, downregulation of cGAS diminished early inflammatory cell infiltration and inflammatory cytokine expression in response to TAC. These results demonstrated that cGAS played an essential pathogenetic role in pressure overload-induced HF to promote pathological cardiac remodeling and dysfunction. Our results suggest that inhibition of cGAS may be a novel therapeutic approach for HF. In this study, we first revealed a novel role of cGAS in the regulation of pathological cardiac remodeling and dysfunction upon pressure overload. We found that the cGAS/STING pathway was activated during pressure overload. Moreover, we also demonstrated that inhibition of the cGAS/STING pathway alleviated pathological cardiac remodeling and downregulated the early inflammatory response during pressure overload-induced HF. Together, these findings will provide a new therapeutic target for HF.
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http://dx.doi.org/10.1152/ajpheart.00097.2020DOI Listing
June 2020

Functional peptide-based drug delivery systems.

J Mater Chem B 2020 08 30;8(31):6517-6529. Epub 2020 Apr 30.

People's Public Security University of China, Beijing 100038, China.

Peptides are one of the most important functional motifs for constructing smart drug delivery systems (DDSs). Functional peptides can be conjugated with drugs or carriers via covalent bonds, or assembled into DDSs via supramolecular forces, which enables the DDSs to acquire desired functions such as targeting and/or environmental responsiveness. In this mini review, we first introduce the different types of functional peptides that are commonly used for constructing DDSs, and we highlight representative strategies for designing smart DDSs by using functional peptides in the past few years. We also state the challenges of peptide-based DDSs and come up with prospects.
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http://dx.doi.org/10.1039/d0tb00713gDOI Listing
August 2020

Diagnosis and Treatment of Hypospadias With Megameatus Intact Prepuce.

Front Pediatr 2020 31;8:128. Epub 2020 Mar 31.

Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, Shantou, China.

To evaluate the diagnosis and treatment methods of hypospadias with megameatus intact prepuce (MIP). A retrospective analysis was performed in 27 MIP children, 13 of whom underwent tubularized incised plate urethroplasty (TIP procedure), 7 underwent the Duplay procedure, 5 underwent the Mathieu procedure, 1 underwent meatal advancement and glanuloplasty (MAGPI procedure), and 1 underwent the glans approximation procedure (GAP). The patients were followed for 6-36 months to evaluate the surgical outcomes by the Pediatric Penile Perception Score (PPPS). A total of 27 patients with a mean age of 8.12 ± 3.0 years were enrolled in this study, and 25 cases (25/27, 92.6%) were accidentally discovered during the first visit for phimosis. The patients had a formed urethra of 0.5 to 1.5 cm. Complications occurred in 4 of the 27 patients (14.81%): 2 patients with urethral fistula and 2 patients with meatal stenosis. One patient had a case of self-healed urethral fistula, and the remaining 3 patients underwent reoperation. The post-operative effect was satisfactory in all patients, and the urinary flow and stream during urination were normal. The overall average PPPS score of non-operative surgeons and parents was satisfactory. There were no significant differences in meatus appearance, glans appearance, skin appearance, and general appearance PPPS score among the Mathieu, TIP, and Duplay surgical procedures. MIP clinical manifestations are concealed and usually noted when circumcision is attempted. The suitable procedure for each patient should be tailored according to the anatomic features, and several techniques can be used with good functional and cosmetic results.
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http://dx.doi.org/10.3389/fped.2020.00128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136419PMC
March 2020

Microfluidic formation of highly monodispersed multiple cored droplets using needle-based system in parallel mode.

Electrophoresis 2020 06 13;41(10-11):891-901. Epub 2020 Feb 13.

Department of Chemical and Environmental Engineering, University of Nottingham Ningbo China, Ningbo, P. R. China.

Scale-up in droplet microfluidics achieved by increasing the number of devices running in parallel or increasing the droplet makers in the same device can compromise the narrow droplet-size distribution, or requires high fabrication cost, when glass- or polymer-based microdevices are used. This paper reports a novel way using parallelization of needle-based microfluidic systems to form highly monodispersed droplets with enhanced production rates yet in cost-effective way, even when forming higher order emulsions with complex inner structure. Parallelization of multiple needle-based devices could be realized by applying commercially available two-way connecters and 3D-printed four-way connectors. The production rates of droplets could be enhanced around fourfold (over 660 droplets/min) to eightfold (over 1300 droplets/min) by two-way connecters and four-way connectors, respectively, for the production of the same kind of droplets than a single droplet maker (160 droplets/min). Additionally, parallelization of four-needle sets with each needle specification ranging from 34G to 20G allows for simultaneous generation of four groups of PDMS microdroplets with each group having distinct size yet high monodispersity (CV < 3%). Up to six cores can be encapsulated in double emulsion using two parallelly connected devices via tuning the capillary number of middle phase in a range of 1.31 × 10 to 4.64 × 10 . This study leads to enhanced production yields of droplets and enables the formation of groups of droplets simultaneously to meet extensive needs of biomedical and environmental applications, such as microcapsules with variable dosages for drug delivery or drug screening, or microcapsules with wide range of absorbent loadings for water treatment.
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http://dx.doi.org/10.1002/elps.201900403DOI Listing
June 2020

Effects of Chronic Ephedrine Toxicity on Functional Connections, Cell Apoptosis, and CREB-Related Proteins in the Prefrontal Cortex of Rhesus Monkeys.

Neurotox Res 2020 Mar 20;37(3):602-615. Epub 2019 Dec 20.

Shantou University Medical College, No. 22 Xinling Road, Shantou, Guangdong, 515041, People's Republic of China.

Ephedrine abuse has spread in many parts of the world, severely threatening human health. The mechanism of ephedrine toxicity is still unclear. To explore the possible neural mechanisms of ephedrine toxicity, this study established a non-human primate model of ephedrine exposure, analyzed the functional connectivity changes in its prefrontal cortex through resting state BOLD-fMRI, and then inspected the pathophysiological changes as well as the expression of the cyclic adenosine monophosphate response element-binding protein (CREB), phosphorylated CREB (P-CREB), and CREB target proteins (c-fos and fosB) in the prefrontal cortex. After ephedrine toxicity, we found that the prefrontal cortex of monkeys strengthened its functional connectivity with the brain regions that perform motivation, drive, reward, and learning and memory functions and weakened its functional connectivity with the brain regions that perform cognitive control. These results suggest that ephedrine toxicity causes abnormal neural circuits that lead to the amplification and enhancement of drug-related cues and the weakening and damage of cognitive control function. Histology showed that the neurocytotoxicity of ephedrine can cause neuronal degeneration and apoptosis. Real-time PCR and Western blot showed increased expression of CREB mRNA and CREB/P-CREB/c-fos/fosB protein in the prefrontal cortex after ephedrine toxicity. Collectively, the present study indicates that the enhancement of drug-related cues and the weakening of cognitive control caused by abnormal neural circuits after drug exposure may be a major mechanism of brain function changes caused by ephedrine. These histological and molecular changes may be the pathophysiological basis of brain function changes caused by ephedrine.
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http://dx.doi.org/10.1007/s12640-019-00146-3DOI Listing
March 2020

Association between polymorphisms in interleukin-18 promoter and risk of coronary artery disease: a meta-analysis.

Biosci Rep 2019 11;39(11)

Department of Cardiology, Peking University People's Hospital, Beijing, China.

Background: Previous studies have explored associations between interleukin-18 (IL-18) promoter polymorphisms and coronary artery disease (CAD). However, the results were controversial. We conducted a meta-analysis to clarify the association between the two polymorphisms and CAD risk.

Methods: We searched English and Chinese databases and calculated the odds ratio (OR) and 95% confidence interval (CI) to estimate whether there are genetic associations between IL-18 promoter polymorphisms and the risk of CAD. All relevant studies were screened and meta-analyzed using STATA 15.0.

Results: A total of 15 studies, including 12 studies for -137 G/C and 9 studies for -607 C/A, were identified for the meta-analysis. For -137 G/C, the results showed a significantly reduced risk of CAD in the dominant model (OR = 0.85) and heterozygous model (OR = 0.88) in the overall analysis. However, in subgroup analysis, decreased CAD risks were only observed in Asian populations for heterozygous genetic models. For -607 C/A, the overall OR revealed a reduced risk of CAD in all five genetic models (allelic, OR = 0.78; recessive, OR = 0.75; dominant, OR = 0.68; homozygous, OR = 0.61; heterozygous, OR = 0.72). In subgroup analysis, reduced CAD risk was also found in five genetic models of the Asian population. We also found that the IL-18 polymorphisms were correlated with myocardial infarction (MI) and multivessel (MV) disease.

Conclusion: Our results suggested that the -137 polymorphism and -607 polymorphism in the IL-18 promoter were negatively associated with CAD, especially in the Asian population. In addition, some genetic models were correlated with the severity of CAD.
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http://dx.doi.org/10.1042/BSR20192721DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6863765PMC
November 2019

Newtonian flow inside carbon nanotube with permeable boundary taking into account van der Waals forces.

Sci Rep 2019 Aug 20;9(1):12121. Epub 2019 Aug 20.

Department of Mechanical, Materials and Manufacturing Engineering, Faculty of Science and Engineering, The University of Nottingham Ningbo China, 199 Taikang East Road, Ningbo, China.

Here, water flow inside large radii semi-infinite carbon nanotubes is investigated. Permeable wall taking into account the molecular interactions between water and a nanotube, and the slip boundary condition will be considered. Furthermore, interactions among molecules are approximated by the continuum approximation. Incompressible and Newtonian fluid is assumed, and the Navier-Stokes equations, after certain assumptions, transformations and derivations, can be reduced into two first integral equations. In conjunction with the asymptotic expansion technique, we are able to derive the radial and axial velocities analytically, capturing the effect of the water leakage, where both mild and exceptionally large leakages will be considered. The radial velocity obeys the prescribed boundary condition at the (im)permeable wall. Through the mean of the radial forces, the sufficiently large leakages will enhance the radial velocity at the center of the tube. On the other hand, unlike the classical laminar flow, the axial velocity attains its maximum at the wall due to the coupling effect with the radial forces as water is being pushed into the proximity of the inner wall. In addition, the axial velocity and the flux with the consideration of the suck-in forces, induced by the tubes' entry turn out to be one order higher than that without the suck-in forces. All the aforementioned considerations might partially resolve the mysteriously high water penetration through nanotubes. Axial velocity also drops with the tube's length when the water leakage is permitted and the suck-in forces will ease the decline rate of the axial velocity. The present mathematical framework can be directly employed into the water flow inside other porous nano-materials, where large water leakage is permitted and therefore are of huge practical impact on ultra-filtration and environmental protection.
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http://dx.doi.org/10.1038/s41598-019-48614-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6702207PMC
August 2019

Methods for estimating fragment hazard in gas explosion.

Authors:
Zheng Lian Qi Zhang

J Hazard Mater 2020 01 9;381:120989. Epub 2019 Aug 9.

State Key Laboratory of Explosion Science and Technology, Beijing Institute of Technology, Beijing 100081, China. Electronic address:

Fragmention is a principal factor for casualties in an indoor fuel gas explosion. This paper presents a predictive method for the spatial distribution of hazardous fragments and areas. The motion parameters of fragments in indoor explosions were obtained by a combination of fluid and structural dynamic analysis. Then the spatial distribution of falling points was obtained by solving the motion equation of fragments. Indoor obstacle is an important factor affecting the hazard of indoor explosions. In the case of a vented brick room measuring 4.6 m × 4.6 m × 3 m, with the ratio of obstacle volume to gas volume increasing from 0.24 to 0.36, the maximum launch distance increased from 6.18 m to 18.78 m, expanded to 3.04 times. The fragment hazards in indoor explosions of methane, hydrogen and LPG (Liquefied Petroleum Gas) with the same condition were compared. The hazard distance of hydrogen explosion is the largest, which is 3.11 times as wide as that of methane.
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http://dx.doi.org/10.1016/j.jhazmat.2019.120989DOI Listing
January 2020

Lymphatic system identification, pathophysiology and therapy in the cardiovascular diseases.

J Mol Cell Cardiol 2019 08 7;133:99-111. Epub 2019 Jun 7.

Department of Cardiology, Beijing Key Laboratory of Early Prediction and Intervention of Acute Myocardial Infarction, Center for Cardiovascular Translational Research, Peking University People's Hospital, Beijing, China. Electronic address:

The mammalian circulatory system comprises both the cardiovascular system and the lymphatic system. In contrast to the closed, high-pressure and circular blood vascular circulation, the lymphatic system forms an open, low-pressure and unidirectional transit network from the extracellular space to the venous system. It plays a key role in regulating tissue fluid homeostasis, absorption of gastrointestinal lipids, and immune surveillance throughout the body. Despite the critical physiological functions of the lymphatic system, a complete understanding of the lymphatic vessels lags far behind that of the blood vasculatures due to the challenge of their visualization. During the last 20 years, discoveries of underlying genes responsible for lymphatic vessel biology, combined with state-of-the-art lymphatic function imaging and quantification techniques, have established the importance of the lymphatic vasculature in the pathogenesis of cardiovascular diseases including lymphedema, obesity and metabolic diseases, dyslipidemia, hypertension, inflammation, atherosclerosis and myocardial infraction. In this review, we highlight the most recent advances in the field of lymphatic vessel biology, with an emphasis on the new identification techniques of lymphatic system, pathophysiological mechanisms of atherosclerosis and myocardial infarction, and new therapeutic perspectives of lymphangiogenesis.
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http://dx.doi.org/10.1016/j.yjmcc.2019.06.002DOI Listing
August 2019

Association of early menopause with angiographically-derived SYNTAX score: Observational study.

Medicine (Baltimore) 2018 Dec;97(51):e13723

Department of Cardiology.

Association of early menopause with increased risk of cardiovascular events has been confirmed in previous studies. SYNTAX score (SX-score) can comprehensively quantify severity of coronary artery disease (CAD) and predict the outcomes of patients with CAD. However, the association of early menopause with SX-score has never been reported.We prospectively included 1875 consecutive postmenopausal patients who underwent coronary angiography (CAG) and were angiographically diagnosed with CAD from January 2011 to December 2013. SX-score was calculated using the SX-score algorithm based on diagnostic angiogram. Ordinal logistic regression analysis was used to investigate the association between early menopause and SX-score.Patients with early menopause were more likely to have a history of hypertension, diabetes, hyperlipidemia, and less likely to smoking. Besides, they have higher fasting glucose, hemoglobin A1C (HbA1c), total cholesterol (TC), low-density lipoprotein (LDL), triglyceride (TG), and body mass index (BMI) compared with the patients without early menopause. Moreover, patients with early menopause have higher SX-score and multi-vessel diseases. Ordinal logistic regression analysis showed that age, hypertension, diabetes, and early menopause exerted independent influences on SX-score. The patients undergone oophorectomy, early menopause was highly associated with SX-score.Early menopause was an independent predictor of SX-score in postmenopausal patients with CAD.
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http://dx.doi.org/10.1097/MD.0000000000013723DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6319991PMC
December 2018

Weighted gene co‑expression network analysis in identification of key genes and networks for ischemic‑reperfusion remodeling myocardium.

Mol Med Rep 2018 Aug 14;18(2):1955-1962. Epub 2018 Jun 14.

Department of Cardiology, Cangzhou Central Hospital, Hebei Medical University, Cangzhou, Hebei 061000, P.R. China.

Acute myocardial infarction induces ventricular remodeling, which is implicated in dilated heart and heart failure. The pathogenical mechanism of myocardium remodeling remains to be elucidated. The aim of the present study was to identify key genes and networks for myocardium remodeling following ischemia‑reperfusion (IR). First, the mRNA expression data from the National Center for Biotechnology Information database were downloaded to identify differences in mRNA expression of the IR heart at days 2 and 7. Then, weighted gene co‑expression network analysis, hierarchical clustering, protein‑protein interaction (PPI) network, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway were used to identify key genes and networks for the heart remodeling process following IR. A total of 3,321 differentially expressed genes were identified during the heart remodeling process. A total of 6 modules were identified through gene co‑expression network analysis. GO and KEGG analysis results suggested that each module represented a different biological function and was associated with different pathways. Finally, hub genes of each module were identified by PPI network construction. The present study revealed that heart remodeling following IR is a complicated process, involving extracellular matrix organization, neural development, apoptosis and energy metabolism. The dysregulated genes, including SRC proto‑oncogene, non‑receptor tyrosine kinase, discs large MAGUK scaffold protein 1, ATP citrate lyase, RAN, member RAS oncogene family, tumor protein p53, and polo like kinase 2, may be essential for heart remodeling following IR and may be used as potential targets for the inhibition of heart remodeling following acute myocardial infarction.
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http://dx.doi.org/10.3892/mmr.2018.9161DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072198PMC
August 2018

The anti-inflammatory effect of microRNA-383-3p interacting with IL1R2 against homocysteine-induced endothelial injury in rat coronary arteries.

J Cell Biochem 2018 08 25;119(8):6684-6694. Epub 2018 Apr 25.

The First Department of Cardiovascular Medicine, Cangzhou Central Hospital, Cangzhou, Hebei Province, P. R. China.

MicroRNAs (miRs) are widely reported to be novel biomarkers involved in the process of coronary atherosclerosis (CAS). Hence, this study aims to explore the function of miR-383-3p targeting IL1R2 on inflammatory injury of coronary artery endothelial cells (CAECs) in CAS. The underlying regulatory mechanisms of miR-383-3p were analyzed in concert with the treatment of miR-383-3p mimics, miR-383-3p inhibitors, and the combination of miR-383-3p inhibitors and siRNA against IL1R2 in homocysteine (HCY)-induced CAECs. MTT, Hoechst 33258 staining, and tube formation assay were employed in order to measure cell viability, apoptosis, and tube formation, respectively. The levels of IL-1β, IL-6, IL-10, and IL-18 were determined by ELISA. IL1R2 was verified as the target gene of miR-383-3p by dual-luciferase reporter gene assay. MiR-383-3p was down-regulated in myocardial tissues of AS rats while IL1R2 was the reciprocal. The up-regulation of miR-383-3p decreased the levels of IL1R2, caspase-1, IL-1β, IL-6, and IL-18 expressions, as well as cell apoptosis rate in the HCY-induced CAECs, while IL-10 expression, cell viability, and tube formation ability were increased. These results were contraindicated in the HCY-induced CAECs treated by miR-383-3p inhibitors. In conclusion, miR-383-3p mediating IL1R2 prevents HCY-induced apoptosis and inflammation injury in CAECs through the inhibition of the activation of inflammasome signaling pathway. These findings highly indicate that miR-383-3p may be beneficial in the prevention of CAS and other cardiovascular diseases.
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http://dx.doi.org/10.1002/jcb.26854DOI Listing
August 2018

A double projection algorithm for quasimonotone variational inequalities in Banach spaces.

Authors:
Lian Zheng

J Inequal Appl 2018 24;2018(1):256. Epub 2018 Sep 24.

Department of Mathematics and Statistics, Yangtze Normal University, Chongqing, China.

We propose a double projection algorithm for solving variational inequality problems in Banach spaces. We establish the strong convergence of the whole sequence generated by the proposed method under the quasimonotone and uniform continuity on bounded sets, which are weaker conditions than those used in existing projection-type methods for solving variational inequality problems in Banach spaces.
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http://dx.doi.org/10.1186/s13660-018-1852-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154076PMC
September 2018

Insecticidal activities of constituents of Litsea cubeba fruit extracts effective against the maize weevil (Coleoptera: Curculionidae).

J Insect Sci 2017 Sep;17(5)

Hainan Institute of Grain and Oil Science, Qionghai 571400, China.

In this study, we investigated the insecticidal activities, including contact toxicity, fumigant toxicity, and repellent activity, of Litsea cubeba fruit extracts against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae). The extracts, obtained by liquid-liquid extraction in n-hexane, ethyl acetate, chloroform, and water were analyzed by gas chromatography-mass spectrometry. Among the different extract types, chloroform extracts exhibited the strongest repellent, contact, and fumigant activities against S. zeamais. The main components of the chloroform extracts were identified as laurine (21.15%) and 2,6-diisopropyl aniline (16.14%), followed by chlorobutanol (10.54%), 3-O-methyl-N-acetyl-d-glucosamine (10.03%), and 6-methyl-5-hepten-2-one (8.33%). Among the identified components of the chloroform extracts, chlorobutanol showed the strongest fumigant toxicity (LD50 = 21.91 mg/liter), contact toxicity (LD50 = 54.25 µg/adult), and repellent activity against S. zeamais. These results indicate that L. cubeba fruit extracts possess natural insecticide-like activities against S. zeamais.
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http://dx.doi.org/10.1093/jisesa/iex079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639732PMC
September 2017

[Methods and effects of injection of salvianolic acid B combined with triamcinolone acetonide in the treatment of oral submucous fibrosis].

Shanghai Kou Qiang Yi Xue 2017 Apr;26(2):188-192

Department of Oncology, the Third Xiangya Hospital, Central South University. Changsha 410013, Hunan Province, China.

Purpose: To introduce the methods of triamcinolone acetonide (TA) and salvianolic acid B(SA-B) intralesional injection in the treatment of oral submucous fibrosis and evaluate the treatment effects.

Methods: TA combined with SA-B were consecutively applied intralesionally 1 time weekly for 30 times. The technique of intralesional injection was that syringe needle be emptied into submucous tissues 1 cm posterior to the corner of oral cavity running from the front to retromolar area by pushing the injection, then the syringe needle retained to the original point and upward from the front to retromolar area by pushing the injection, finally the syringe needle returned to the starting point and downward from the front to retromolar area by pushing the injection. Mouth opening, the color change of the buccal mucosa and the increase of capillary vessels as determined by degree I-IV visual analog scale were evaluated at 12, 24, and 36 month. The data were analyzed with SPSS 21.0 software package.

Results: One hundred and fourteen subjects fulfilled the study without obvious adverse reactions. After treatment for 36 months , the net gain in mouth opening of the early stage group,middle stage group and advanced stage group was (12.0±1.2) mm, (14.5±2.4) mm and (15.5±1.5) mm, respectively. The response rate of color change of the buccal mucosa and the increase of capillary vessels in early stage group, middle stage group and advanced stage group after treatment for 36 months was 100%, 94.7% and 90.7%, respectively.

Conclusions: Triamcinolone acetonide and salvianolic acid B intralesional injection is effective in the treatment of oral submucous fibrosis.
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April 2017

Tanshinone Suppresses Arecoline-Induced Epithelial-Mesenchymal Transition in Oral Submucous Fibrosis by Epigenetically Reactivating the p53 Pathway.

Oncol Res 2018 Apr 21;26(3):483-494. Epub 2017 May 21.

Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P.R. China.

Oral submucous fibrosis (OSF) induced by chewing of the areca nut has been considered to be a precancerous lesion with a high probability of developing oral squamous cell carcinoma. Tanshinone (TSN) is the main component extracted from , a traditional Chinese medicine, which was found to have diverse pharmacological effects, such as anti-inflammatory and antitumor. In the current study, we aimed to identify the inhibitory effects and the underlying mechanism of TSN on OSF progress. We found that treatment with TSN inhibited the arecoline-mediated proliferation of primary human oral mucosal fibroblasts and reversed the promotive effects of arecoline on the EMT process. By RNA deep sequencing, we screened two possible targets for TSN: LSD1 and p53. We confirmed that p53 is much lower in OSF than in normal mucous tissues. In addition, p53 and its downstream molecules were decreased by arecoline treatment in oral mucosal fibroblasts, which was reversed by treatment with TSN in a dose-dependent manner. Our results also revealed that arecoline stimulation resulted in hypermethylation of the promoter of and subsequent downregulation of p53 levels, which was reversed by TSN. Furthermore, we identified that LSD1 could epigenetically activate by recruiting H3K27me1 and H3K4m2 to its promoter. Our findings provide new insights into the mechanism by which TSN influences arecoline-induced OSF and rationale for the development of clinical intervention strategies for OSF and even oral squamous cell carcinoma.
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http://dx.doi.org/10.3727/096504017X14941825760362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844836PMC
April 2018

Lotus seedpod as a low-cost biomass for potential methylene blue adsorption.

Water Sci Technol 2016 Dec;74(11):2560-2568

Central and Southern China Municipal Engineering Design & Research Institute Co. Ltd, Wuhan 430072, China.

The adsorption of methylene blue (MB) by low cost biomass lotus seedpod (LSP) was optimized by a central composite design combined with response surface methodology in aqueous solution. Solution pH, initial dye concentration and adsorbent dosage were studied as independent variables at five levels each, respectively. Analysis of variance suggested the validity of the regression model. LSP was characterized by Fourier transform infrared spectra and energy dispersive spectroscopy. The kinetics revealed that the adsorption behavior followed the pseudo-second-order model. Langmuir and Freundlich isotherm models were used to evaluate the adsorption, and the experimental data were better fitted by the Langmuir isotherm than the Freundlich isotherm. The maximum monolayer adsorption capacity of the LSP was 157.98 mg g at 30 °C for MB adsorption. In addition, 0.2 M HCl solution could be used for reusability of LSP via desorption tests. LSP was proven to be an available and effective biosorbent for MB removal from aqueous solution.
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http://dx.doi.org/10.2166/wst.2016.423DOI Listing
December 2016
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