Publications by authors named "Li-Xing Nie"

12 Publications

  • Page 1 of 1

Micro-morphological identification study on Cordyceps sinensis (Berk.) Sacc. and its adulterants based on stereo microscope and desktop scanning electron microscope.

Microsc Res Tech 2021 Apr 1. Epub 2021 Apr 1.

Institute for Quality Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, China.

The Chinese Materia Medica, Cordyceps sinensis (called "Dongchongxiacao" in Chinese), used as a tonic for nearly 600 years by Traditional Chinese Medicine, which has been recorded by Chinese Pharmacopoeia. This drug is rare and precious, which in turn lead to the emergence of adulterants derived from the same genus of Cordyceps. The adulterants which can be commonly found in the market are Cordyceps gunnii (called "Gunichongcao" in Chinese), Cordyceps liangshanensis (called "Liangshanchongcao" in Chinese), and Cordyceps gracilis (called "Xinjiangchongcao" in Chinese). This study combined a desktop scanning electron microscope and stereo microscope to distinguish C. sinensis from the above three adulterants especially on their different characters of caterpillar parts. Referring to the professional entomological literature, the micro-morphological features including the cuticle of the abdomen and the planta of abdomen prolegs were observed, photographed, and expressed based on the description of macroscopic characters. The identification method studied in this article is more convenient, quick, and environmental friendly.
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http://dx.doi.org/10.1002/jemt.23749DOI Listing
April 2021

Antiviral activity of Isatidis Radix derived glucosinolate isomers and their breakdown products against influenza A in vitro/ovo and mechanism of action.

J Ethnopharmacol 2020 Apr 7;251:112550. Epub 2020 Jan 7.

Chinese Academy of Medical Science & Peking Union Medical College, Beijing, PR China; National Institutes for Food and Drug Control, National Medical Products Administration, Beijing, PR China. Electronic address:

Ethnopharmacological Relevance: Isatidis Radix, the sun-dried roots of Isatis indigotica Fortune ex Lindl., is one of the most usually used traditional Chinese medicines. For centuries, the herb has been employed in clinical practice for treatment of virus infection and inflammation. However, its active ingredients remain unclear.

Aim Of The Study: In the present study, the anti-influenza virus activity of epiprogoitrin, progoitrin, epigoitrin and goitrin, the Isatidis Radix derived glucosinolate isomers and their breakdown products, was firstly evaluated in vitro and in ovo and their mechanism of action was investigated.

Materials And Methods: Epiprogoitrin, progoitrin, epigoitrin and goitrin were isolated from Isatidis Radix by chiral separation. In vitro and in ovo evaluations were performed on Madin-Darby canine kidney (MDCK) cells and embryonated eggs respectively, both using protocols including prevention, treatment and virus neutralization. Hemagglutination (HA) and neuraminidase (NA) inhibition assays were performed for further understanding of the antiviral mechanism.

Results: Isatidis Radix derived glucosinolate isomers and their breakdown products all exhibited dose-dependent inhibition effect against influenza A virus (H1N1) without toxicity. The antiviral potency of the components was in the order of progoitrin > goitrin > epigoitrin > epiprogoitrin. The attachment of the constituents to the viral envelope conduced to the mechanism of their antiviral action without disturbing viral adsorption or budding.

Conclusion: Taken together, these results are promising for further development of Isatidis Radix and may contribute an adjunct to pharmacotherapy for influenza virus infection.
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http://dx.doi.org/10.1016/j.jep.2020.112550DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126217PMC
April 2020

[Determination and risk assessment of heavy metals and harmful elements residues in Niuhuang Qingwei Pills based on ICP-MS].

Zhongguo Zhong Yao Za Zhi 2019 Jan;44(1):82-87

National Institute for Food and Drug Control Beijing 100050,China.

To achieve a comprehensive understanding of heavy metals and harmful elements residues in Niuhuang Qingwei Pills,49 samples from 18 manufactures were collected from 31 provinces in China.Risk assessment and control preparations were applied innovatively in evaluation of exogenous pollution in traditional Chinese Medicine.Determination methods for Pb,Cd,As,Hg and Cu were established by inductively coupled plasma mass spectrometry(ICP-MS).Based on the procedures including hazard identification,hazard characterization,exposure assessment and risk characterization,risk assessment was performed and residual limits for Pb,Cd,As,Hg and Cu in the drug were formulated.The results showed that the hazardous quotients(HQ) of the elements were decreased in the following order:Pb>As>Cu>Hg>Cd,and the total hazardous index(HI) of heavy metals and harmful elements in Niuhuang Qingwei Pills was above 1,implying health risk of the drug.Under the proposed limits,5 elements in the control preparation as well as Cd and Cu in the samples were within the limits range,but the excess rates of Pb,As and Hg in the samples were 12%,12% and 14%,respectively.For the first time,basic steps for risk assessment of Chinese patent medicine were established,which provided model and reference for risk assessment and limit formulation of other drugs.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20181108.002DOI Listing
January 2019

[Expert consensus on prescription comment of Chinese traditional patent medicine for promoting the rational use of drugs in Beijing].

Zhongguo Zhong Yao Za Zhi 2018 Mar;43(5):1049-1053

Beijing Key Laboratory of Bio-characteristic Profiling for Evaluation of Rational Drug Use, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China.

With the growth of number of Chinese patent medicines and clinical use, the rational use of Chinese medicine is becoming more and more serious. Due to the complexity of Chinese medicine theory and the uncertainty of clinical application, the prescription review of Chinese patent medicine always relied on experience in their respective, leading to the uncontrolled of clinical rational use. According to the traditional Chinese medicine (TCM) theory and characteristics of the unique clinical therapeutics, based on the practice experience and expertise comments, our paper formed the expert consensus on the prescription review of Chinese traditional patent medicine for promoting the rational use of drugs in Beijing. The objective, methods and key points of prescription review of Chinese patent medicine, were included in this expert consensus, in order to regulate the behavior of prescription and promote rational drug use.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.2018.0036DOI Listing
March 2018

[Guideline principle and technical requirement for preparing traditional Chinese medicine reference drug].

Zhongguo Zhong Yao Za Zhi 2017 Oct;42(19):3672-3675

National Institutes for Food and Drug Control, Beijing 100050, China.

Traditional Chinese medicine (TCM) reference drug is a new form of TCM standard reference substance. The purpose of this guideline is to guide the establishment of the reference drug and standardize its investigation and application in national drug standards. Definition of TCM reference drug was specified and relating guideline and technical requirement were introduced in this paper. Its application in quality control of TCM was analyzed and the developing train was proposed. There is a wide prospect for the application of reference drug in quality control of TCM. Thus it has practical significance to explore and conduct the quality evaluation system by using TCM reference drug as the reference substance.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20170901.016DOI Listing
October 2017

[Application of microscopic spectroscopy in quality control of Niuhuang Qingxin pills].

Zhongguo Zhong Yao Za Zhi 2016 Oct;41(20):3741-3745

National Institutes for Food and Drug Control, Beijing 100050, China.

Application of microscopic spectroscopy in quality control of Niuhuang Qingxin pills was discussed. First, microscopic characteristics specified by the statutory standard of Niuhuang Qingxin pills were summarized. Then new identification method was established for Dioscoreae Rhizoma, Saigae Tataricae Cornu, Cinnamomi Cortex and Saposhnikoviae Radix. Finally, microscopic spectroscopy was used for test of Dioscoreae Rhizoma's adulterant Dioscoreae Fordii Rhizoma.It was the first time for this technology being applied in adulteration test of Chinese patent medicine.The results showed that Saigae Tataricae Cornu was not detected in 2 batches of Niuhuang Qingxin pills from 1 manufacturer while Dioscoreae Fordii Rhizoma was detected in 3 batches of samples from 2 manufacturers. The proposed methods were accurate, simple, rapid, objective and economic, which offered a more comprehensive approach for quality control of Niuhuang Qingxin pills. It was indicated that conventional technology such as microscopic spectroscopy could play an important role in identification of traditional Chinese medicine whose index ingredient was deficient or tiny.
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http://dx.doi.org/10.4268/cjcmm20162007DOI Listing
October 2016

[Preliminary investigation on polymorphs of two traditional Chinese medicine reference substances].

Zhongguo Zhong Yao Za Zhi 2015 Aug;40(16):3245-8

Crystal structures of chemical drugs has been being investigated widely. But few attention has been paid to polymorphs-phenomena of active ingredients from Traditional Chinese Medicine(TCM). Taking anhydrous dehydroandrographolide and hydrousprim-O-glucosylcimifugin as example, differences between TCM reference substances (RSs) with different crystal structures were discussed by using microscopy, melting point determination, differential thermal analysis (DTA) and infrared (IR) methods. The results showed that different crystal structures could lead to change of melting points, thermal behaviors and IR spectrum. It's indicated that polymorphs may be considered if different physicochemical properties were obtained when applying TCM RS. Differences of chemical properties of active ingredients from TCM with different crystal structures need further investigation.
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August 2015

Quantitative Analysis of Panax ginseng by FT-NIR Spectroscopy.

J Anal Methods Chem 2014 5;2014:741571. Epub 2014 May 5.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6 Wangjing Zhonghuannan Road, Beijing 100102, China.

Near-infrared spectroscopy (NIRS), a rapid and efficient tool, was used to determine the total amount of nine ginsenosides in Panax ginseng. In the study, the regression models were established using multivariate regression methods with the results from conventional chemical analytical methods as reference values. The multivariate regression methods, partial least squares regression (PLSR) and principal component regression (PCR), were discussed and the PLSR was more suitable. Multiplicative scatter correction (MSC), second derivative, and Savitzky-Golay smoothing were utilized together for the spectral preprocessing. When evaluating the final model, factors such as correlation coefficient (R (2)) and the root mean square error of prediction (RMSEP) were considered. The final optimal results of PLSR model showed that root mean square error of prediction (RMSEP) and correlation coefficients (R (2)) in the calibration set were 0.159 and 0.963, respectively. The results demonstrated that the NIRS as a new method can be applied to the quality control of Ginseng Radix et Rhizoma.
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http://dx.doi.org/10.1155/2014/741571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4026986PMC
June 2014

[Nondestructive identification of the root of mountain cultivation ginseng and growth years by near infrared spectroscopy].

Guang Pu Xue Yu Guang Pu Fen Xi 2012 Jul;32(7):1801-5

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.

Mountain cultivation ginseng (MCG) and garden cultivation ginseng (GCCG) were identified by near infrared spectroscopy, so were MCG of different growth years. 96 MCG samples of different growth years, including 24 of fifteen years and 72 of ten years, and 177 GCG samples were collected. After the near infrared spectra of these samples were collected, discriminant analysis was used to distinguish MCG and GCG, so was MCG of different years. After the original spectra were pretreated, discriminant analysis models of MCG and GCG, MCG of different growth years were developed respectively with selected principa component numbers in full spectra region. The correct discrimination rate of two groups of model was both 100%. The propose methods are accurate, fast and nondestructive, and can be applied to the quality control of MCG. It has an important significance for building market image of MCG.
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July 2012

Rapid discrimination of Chinese red ginseng and Korean ginseng using an electronic nose coupled with chemometrics.

J Pharm Biomed Anal 2012 Nov 12;70:605-8. Epub 2012 Jun 12.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 6 Wangjing Zhonghuan nan Road, Beijing 100102, PR China.

Red ginseng is a precious and widely used traditional Chinese medicine. At present, Chinese red ginseng and Korean ginseng are both commonly found on the market. To rapidly and nondestructively discriminate between Chinese red ginseng and Korean ginseng, an electronic nose coupled with chemometrics was developed. Different red ginseng samples, including Chinese red ginseng (n=30) and Korean ginseng (South Korean red ginseng and North Korean red ginseng n=26), were collected. The metal oxide sensors on an electronic nose were used to measure the red ginseng samples. Multivariate statistical analyses, including principal component analysis (PCA), discriminant factorial analysis (DFA) and soft independent modeling of class analogy (SIMCA), were employed. All of the samples were analyzed by PCA. Most of the samples were used to set up DFA and SIMCA models, and then the remaining samples (Nos. 9, 10, 17, 18, 29, 30, 34, 43, 44, 50, and 51) were projected onto the DFA and SIMCA models in the form of black dots to validate the models. The results indicated that Chinese red ginseng and Korean ginseng were successfully discriminated using the electronic nose coupled with PCA, DFA and SIMCA. The checking scores of the DFA and SIMCA models were 100. The samples projected onto the DFA and SIMCA models were all correctly discriminated. The DFA and SIMCA models were robust. Electronic nose technology is a rapid, accurate, sensitive and nondestructive method to discriminate between Chinese red ginseng and Korean ginseng.
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http://dx.doi.org/10.1016/j.jpba.2012.06.009DOI Listing
November 2012

Simultaneous determination of ginsenosides and lignans in Sheng-mai injection by ultra-performance liquid chromatography with diode array detection.

Pharmazie 2012 Jan;67(1):14-9

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.

An ultra-performance liquid chromatography (UPLC) method with diode array detection was developed for simultaneous analysis of eight ginsenosides (ginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, Rb3, Rd) and one lignan (schizandrin) in Sheng-mai injection, a traditional Chinese medicine prescription widely used for the treatment of cardiovascular diseases. The chromatographic separation was performed on a Waters ACQUITY UPLC HSS T3 column (1.8 microm, 100 mm x 2.1 mm i.d.) using a linear gradient elution over 28 min with a mixture of water and acetonitrile as the mobile phase. All calibration curves showed good linearity (r2 > 0.9998) within the test ranges. Validation proved the repeatability of the method was good and recovery was satisfactory. The validated method was successfully applied to 12 batches of Sheng-mai injection. The results showed that there was a great variation among different samples. Principal component analysis (PCA) further proved considerable variations among the samples from different factories and suggested that schizandrin, ginsenosides Rb1 and Rg1 might have the greatest influence on the variation of 12 samples. In conclusion, these results demonstrated that the UPLC method proposed was very useful for the analysis and quality evaluation of Sheng-mai injection.
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January 2012

[Study of determination method for heavy metals and harmful elements residues in four traditional Chinese medicine injections].

Zhongguo Zhong Yao Za Zhi 2008 Dec;33(23):2764-7

National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China.

Methods for determination of heavy metals and harmful residues in traditional Chinese medicine injection were established. Graphite furnace atomic absorption spectrometry was used for determination of lead, cadmium and copper, atomic fluorescence spectrometry for arsenic and mercury. The preprocessing method was optimized. The average recoveries of 5 elements were between 91% and 112% while the precisions were less than 2%. The determination limit of lead, cadmium, copper, arsenic and mercury were 0.28, 0.014, 0.49, 0.19, 0.061 microg x L(-1), respectively. The proposed method was simple, sensitive, accurate and reliable, and could be used widely.
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December 2008