Publications by authors named "Li-Wei Zhou"

30 Publications

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Species Diversity With Comprehensive Annotations of Wood-Inhabiting Poroid and Corticioid Fungi in Uzbekistan.

Front Microbiol 2020 9;11:598321. Epub 2020 Dec 9.

State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

Uzbekistan, located in Central Asia, harbors high diversity of woody plants. Diversity of wood-inhabiting fungi in the country, however, remained poorly known. This study summarizes the wood-inhabiting basidiomycte fungi (poroid and corticoid fungi plus similar taxa such as , and ) (Agaricomycetes, Basidiomycota) that have been found in Uzbekistan from 1950 to 2020. This work is based on 790 fungal occurrence records: 185 from recently collected specimens, 101 from herbarium specimens made by earlier collectors, and 504 from literature-based records. All data were deposited as a species occurrence record dataset in the Global Biodiversity Information Facility and also summarized in the form of an annotated checklist in this paper. All 286 available specimens were morphologically examined. For 138 specimens, the 114 ITS and 85 LSU nrDNA sequences were newly sequenced and used for phylogenetic analysis. In total, we confirm the presence of 153 species of wood-inhabiting poroid and corticioid fungi in Uzbekistan, of which 31 species are reported for the first time in Uzbekistan, including 19 that are also new to Central Asia. These 153 fungal species inhabit 100 host species from 42 genera of 23 families. Polyporales and Hymenochaetales are the most recorded fungal orders and are most widely distributed around the study area. This study provides the first comprehensively updated and annotated the checklist of wood-inhabiting poroid and corticioid fungi in Uzbekistan. Such study should be expanded to other countries to further clarify species diversity of wood-inhabiting fungi around Central Asia.
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http://dx.doi.org/10.3389/fmicb.2020.598321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756097PMC
December 2020

Basidioradulum mayi and B. tasmanicum spp. nov. (Hymenochaetales, Basidiomycota) from both sides of Bass Strait, Australia.

Sci Rep 2020 01 9;10(1):102. Epub 2020 Jan 9.

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, P.R. China.

Basidioradulum was morphologically considered to be a synonym of Xylodon. Here, its independence within Hymenochaetales is confirmed from a phylogenetic perspective. Basidioradulum radula, the generic type, is widely distributed in Northern Hemisphere. Two Southern Hemisphere species close to B. radula are newly described as B. mayi and B. tasmanicum, respectively, from Victoria and Tasmania, Australia. Basidioradulum mayi differs from B. radula by lack of cystidia. Moreover, the hymenial surface of B. radula is normally much more strongly hydnoid than that of B. mayi. Basidioradulum tasmanicum is distinct from B. radula and B. mayi by having capitate cystidia, ellipsoid to subglobose basidiospores, and crystal-covered hyphae. Although morphologically distinct, the two new species isolated by Bass Strait have an almost identical ITS region, and could not be differentiated by nLSU- and ITS-based phylogenetic analyses. This case reminds us that basing phylogeny simply on the ITS as a barcode region may underestimate fungal species diversity.
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http://dx.doi.org/10.1038/s41598-019-57061-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6952435PMC
January 2020

Reinstatement of the corticioid genus (Hymenochaetales, Basidiomycota) with a new species from Hubei, Central China.

MycoKeys 2019 8;51:85-96. Epub 2019 May 8.

State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

The monotypic genus was previously considered to be a later synonym of . With the morphological and phylogenetic evidence provided by an additional four East Asian specimens, we propose to reinstate as an independent genus in Hymenochaetales. morphologically differs from by its grandinioid hymenophore with hyphal strands, numerous thick-walled cystidia with an invaginated apical end and narrowly and thick-walled basidia. The phylogeny generated from the current data set of ITS and 28S regions indicates that forms a sister clade to . Besides the generic type in the clade, two specimens, collected from Hubei, Central China, are newly introduced as . This new species is the second species of and differs from the generic type by its shorter basidiospores and distribution in warm-temperate to subtropical areas in East Asia. The additional two specimens, collected from Da Lat, Viet Nam, differ morphologically, both from each other and from known species of , but more samples need to be examined before further taxonomic decisions can be made.
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http://dx.doi.org/10.3897/mycokeys.51.33262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6520329PMC
May 2019

SEanalysis: a web tool for super-enhancer associated regulatory analysis.

Nucleic Acids Res 2019 07;47(W1):W248-W255

Guangdong Province Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China.

Super-enhancers (SEs) have prominent roles in biological and pathological processes through their unique transcriptional regulatory capability. To date, several SE databases have been developed by us and others. However, these existing databases do not provide downstream or upstream regulatory analyses of SEs. Pathways, transcription factors (TFs), SEs, and SE-associated genes form complex regulatory networks. Therefore, we designed a novel web server, SEanalysis, which provides comprehensive SE-associated regulatory network analyses. SEanalysis characterizes SE-associated genes, TFs binding to target SEs, and their upstream pathways. The current version of SEanalysis contains more than 330 000 SEs from more than 540 types of cells/tissues, 5042 TF ChIP-seq data generated from these cells/tissues, DNA-binding sequence motifs for ∼700 human TFs and 2880 pathways from 10 databases. SEanalysis supports searching by either SEs, samples, TFs, pathways or genes. The complex regulatory networks formed by these factors can be interactively visualized. In addition, we developed a customizable genome browser containing >6000 customizable tracks for visualization. The server is freely available at http://licpathway.net/SEanalysis.
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http://dx.doi.org/10.1093/nar/gkz302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6602466PMC
July 2019

Phylogeny of the genus and taxonomic assessment of the group.

Mycologia 2019 May-Jun;111(3):423-444. Epub 2019 Apr 9.

a Beijing Advanced Innovation Center for Tree Breeding by Molecular Design , Beijing Forestry University , Beijing 100083 , China.

The genus of the Hymenochaetaceae is characterized by resupinate to pileate basidiocarps, a dimitic hyphal system with fine crystal aggregates and encrusted generative hyphae in dissepiment edge and tube trama, the presence of hymenial setae, and hyaline, thin-walled, smooth basidiospores. Members of the group are easy to distinguish from other species of because of the moderately large pores, presence of mycelial setae, and large hymenial setae. Here, we explore phylogenetic relationships among 20 species of based on examination of some 90 collections sampled worldwide. Seven new species are recognized in the group-, and -described from China, Costa Rica, Mexico, and the United States. Phylogenetic analyses inferred from DNA sequences of the nuc rDNA internal transcribed spacer (ITS1-5.8S-ITS2 = ITS), D1-D2 domains of nuc 28S rDNA (28S), and translation elongation factor EF-1 alpha () support the group as one of two major clades within comprising nine species worldwide.
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http://dx.doi.org/10.1080/00275514.2019.1570749DOI Listing
April 2020

Taxonomy and phylogeny of Pyrrhoderma: a redefinition, the segregation of Fulvoderma, gen. nov., and identifying four new species.

Mycologia 2018 Sep-Oct;110(5):872-889. Epub 2018 Sep 21.

c Institute of Microbiology, PO Box 61 , Beijing Forestry University , Beijing 100083 , China.

Previously, Pyrrhoderma accommodated two polypore species, P. adamantinum and P. scaurum; however, phylogenetic studies indicated that these two species were not congeneric within the Hymenochaetaceae and that P. adamantinum formed a clade with Phellinidium noxium. To resolve the relationships among the two species of Pyrrhoderma and other related taxa, specimens from China, Costa Rica, Singapore, and Thailand were studied from both morphological and phylogenetic perspectives. A new genus, Fulvoderma, is erected to accommodate F. scaurum comb. nov., and a new species, F. australe (the generic type). Pyrrhoderma is delimited to include the generic type, P. sendaiense (a later synonym of P. adamantinum); two new combinations, P. lamaënse comb. nov., and P. noxium comb. nov.; and three new species, P. hainanense, P. thailandicum, and P. yunnanense. In addition, an undescribed lineage including several specimens from subtropical and tropical forests in China, Costa Rica, Singapore, and Thailand also nested within the Pyrrhoderma clade. However, as the voucher specimens are sterile or almost so, they are not described. The concept of Pyrrhoderma was emended to also accommodate species bearing resupinate, effuse-reflexed basidiocarps, hymenial or hyphoid setae, and non-subglobose basidiospores. Keys to Fulvoderma and Pyrrhoderma are provided.
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http://dx.doi.org/10.1080/00275514.2018.1474326DOI Listing
March 2019

(Hymenochaetales, Basidiomycota), a new species on from Japan and an identification key to worldwide species of .

MycoKeys 2018 14(30):17-30. Epub 2018 Feb 14.

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.

, in the Hymenochaetaceae, is a polypore genus with a worldwide distribution. The new taxon is introduced, which is the first species to originate from Japan. This species grows exclusively on living and is distinguished by annual, sessile basidiocarps that occur in clusters, pileal surface of narrow, concentrically sulcate zones, 6-8 pores per mm, duplex context separated by a black zone, dimitic hyphal system and broadly ellipsoid basidiospores, 3.2-4 × 2.3-3.1 µm. Phylogenetically, is nested within the clade as a distinct terminal lineage with full statistical supports and sister to the clade of , P. cf. pulla and with weak supports. Besides , and , is the fourth species of recorded from Japan. An identification key to all accepted 48 species of is provided.
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http://dx.doi.org/10.3897/mycokeys.30.23235DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5904531PMC
February 2018

Resolution of phylogenetic position of Nigrofomitaceae within Hymenochaetales (Basidiomycota) and sp. nov. (Nigrofomitaceae) from China.

MycoKeys 2018 12(29):1-13. Epub 2018 Jan 12.

Institute of Microbiology, Beijing Forestry University, Beijing 100083, China.

The family Nigrofomitaceae has been considered to be a member of Polyporales and a synonym of Polyporaceae for a long time. However, no molecular evidence supports this taxonomic opinion. For the first time, Nigrofomitaceae is included in a phylogenetic analysis, which shows that this family is separated from Polyporales and nested within Hymenochaetales as a distinct lineage from four well-known families, viz. Hymenochaetaceae, Neoantrodiellaceae, Oxyporaceae and Schizoporaceae. Therefore, Nigrofomitaceae is treated as the fifth family of Hymenochaetales. , the type species of Nigrofomitaceae, was considered to have a pantropical distribution. However, from both morphological and phylogenetic perspectives, the Chinese specimens labelled as are found not to be conspecific with the specimens of from Costa Rica, close to the type locality in Cuba. These Chinese specimens are thus described as a new species . The species diversity of in pantropical region is discussed.
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http://dx.doi.org/10.3897/mycokeys.29.21250DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5804300PMC
January 2018

Phellinopsis lonicericola and P. tibetica spp. nov. (Hymenochaetales, Basidiomycota), from Tibet, with a key to worldwide species.

Mycologia 2017 3;109(2):210-216. Epub 2017 Mar 3.

b Institute of Microbiology, Beijing Forestry University , Beijing 100083 , People's Republic of China.

Phellinopsis (Hymenochaetaceae) was recently segregated from Phellinus. Phellinopsis differs from other genera segregated from Phellinus in a combination of hymenial setae, if present, mostly originating from tramal hyphae and hyaline basidiospores becoming pale yellowish with age. In this study, two new species of Phellinopsis, P. lonicericola and P. tibetica, from Tibet in southwestern China were described and illustrated. Morphological data and sequences of the nuc rDNA ITS1-5.8S-ITS2 region (internal transcribed spacer [ITS] barcode) support the recognition of these taxa as distinct. They are morphologically compared with other species of Phellinopsis. Notably, P. lonicericola was often found on Lonicera together with Sanghuangporus alpinus, which was originally described from the same locality. In addition, Phellinopsis helwingiae, known from a single collection from Sichuan Province in midwestern China, is reported from Tibet. Eight accepted species of Phellinopsis worldwide, each forming a terminal lineage, grouped together as a strongly supported clade distinct from the clade representing Phellinus. A key to the eight species of Phellinopsis is provided.
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http://dx.doi.org/10.1080/00275514.2017.1300086DOI Listing
October 2018

Phylogeny and diversity of Fomitiporella (Hymenochaetales, Basidiomycota).

Mycologia 2017 15;109(2):308-322. Epub 2017 Mar 15.

a Institute of Microbiology, PO Box 61, Beijing Forestry University , Beijing 100083 , People's Republic of China.

Fomitiporella accommodates polypores producing annual to perennial basidiocarps with an indistinct subiculum (very thin to almost lacking), mostly a dimitic hyphal structure, lacking any kind of setae, with brownish, thick-walled basidiospores, and causing a white rot. Previously, only a few samples of Fomitiporella were studied on the basis of morphological and nuc 28S rDNA (28S)-based phylogenetic analyses. In this study, we made a comprehensive study on Fomitiporella on the basis of collections from Central America, USA, Europe, and China. The phylogenetic analysis, including 28 nuc 28S rDNA and 29 nuc rDNA ITS1-5.8S-ITS2 (internal transcribed spacer [ITS]) sequences newly generated, discovered 14 new lineages. Combined with morphological evidence, 4 new lineages are described and illustrated as new species, viz., Fomitiporella americana, F. micropora, F. sinica, and F. subinermis; 10 other new lineages, each with a single collection, are still treated as unidentified taxa; three new combinations, viz., Fomitiporella tenuissima, F. chinensis, and F. resupinata, are proposed. In addition, F. inermis is redescribed. A key to the 12 known species of Fomitiporella is provided.
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http://dx.doi.org/10.1080/00275514.2017.1305943DOI Listing
October 2018

Global diversity and phylogeny of Onnia (Hymenochaetaceae) species on gymnosperms.

Mycologia 2017 4;109(1):27-34. Epub 2017 Jan 4.

a Institute of Microbiology, PO Box 61, Beijing Forestry University , Beijing , China.

Onnia includes white rotting polypores with annual basidiocarps, a duplex context, monomitic hyphal structure, hymenial setae, and hyaline, thin-walled, smooth basidiospores. Specimens of Onnia, originating mainly from East Asia, Europe, and North America, were studied using both morphology and phylogenetic analyses. Our concatenated data set was derived from 25 collections and included (i) 25 nuc rDNA internal transcribed spacer region sequences (ITS1-5.8S-ITS2 = ITS), 17 generated in this study; and (ii) 14 nuc rDNA 28S rDNA sequences, including the D1-D2 domains, 11 of them generated in this study. The resulting maximum likelihood and Bayesian phylogenies recovered all sampled collections of Onnia as a well-supported clade. In this clade, three previously accepted species, viz., Onnia leporina, O. tomentosa, and O. triquetra, received strong support, whereas three additional lineages with strong support represent the new species described in this paper, O. subtriquetra, O. microspora, and O. tibetica. Of the six Onnia species occurring on gymnosperms, O. tomentosa and O. leporina grow mainly on Picea and have circumboreal distribution in the Northern Hemisphere. In contrast, other species that mostly grow on Pinus are geographically restricted to limited regions, viz., O. triquetra in Europe, O. subtriquetra in North America, and O. microspora and O. tibetica in Asia.
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http://dx.doi.org/10.1080/00275514.2016.1274619DOI Listing
May 2018

Dysregulated miR34a/diacylglycerol kinase ζ interaction enhances T-cell activation in acquired aplastic anemia.

Oncotarget 2017 Jan;8(4):6142-6154

Department of Hematology, Qilu Hospital, Shandong University, Jinan, China.

Acquired aplastic anemia is an idiopathic paradigm of human bone marrow failure syndrome, which involves active destruction of hematopoietic stem cells and progenitors by cytotoxic T cells in the bone marrow. Aberrant expression of microRNAs in T cells has been shown to lead to development of certain autoimmune diseases. In the present study, we performed a microarray analysis of miRNA expression in bone marrow CD3+ T cells from patients with aplastic anemia and healthy controls. Overexpression of miR34a and underexpression of its target gene diacylglycerol kinase (DGK) ζ in bone marrow mononuclear cells were validated in 41 patients and associated with the severity of aplastic anemia. Further, the level of miR34a was higher in naïve T cells from patients than from controls. The role of miR34a and DGKζ in aplastic anemia was investigated in a murine model of immune-mediated bone marrow failure using miR34a-/- mice. After T-cell receptor stimulation in vitro, lymph node T cells from miR34a-/- mice demonstrated reduced activation and proliferation accompanied with a less profound down-regulation of DGKζ expression and decreased ERK phosphorylation compared to those from wild-type C57BL6 control mice. Infusion of 5 × 106 miR34a-/- lymph node T cells into sublethally irradiated CB6F1 recipients led to increased Lin-Sca1+CD117+ cells and less vigorous expansion of CD8+ T cells than injection of same number of wild-type lymph node cells. Our study demonstrates that the miR34a/DGKζ dysregulation enhances T-cell activation in aplastic anemia and targeting miR34a may represent a novel molecular therapeutic approach for patients with aplastic anemia.
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http://dx.doi.org/10.18632/oncotarget.14046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5351619PMC
January 2017

Taxonomy and phylogeny of Phellinidium (Hymenochaetales, Basidiomycota): A redefinition and the segregation of Coniferiporia gen. nov. for forest pathogens.

Fungal Biol 2016 08 12;120(8):988-1001. Epub 2016 May 12.

Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, PR China. Electronic address:

Phellinidium, including 13 accepted polypore species mostly with resupinate basidiocarps, is one of the most aggressive forest pathogenic genera. This genus is characterized by the combination of a monomitic hyphal structure, abundant hyphoid setae in the context and trama, and hyaline and thin-walled basidiospores. To explore the relationships among the species of Phellinidium, especially those between forest pathogens and saprophytes, we examined 29 specimens representing all 13 previously known species from Asia, Europe and America from morphological and phylogenetic perspectives. A new genus, Coniferiporia, was found to segregate from Phellinidium for three aggressive forest pathogens, and three new combinations, viz. Coniferiporia qilianensis (the generic type), Coniferiporia weirii and Coniferiporia sulphurascens, were proposed. Phellinidium cryptocystidiatum was treated as a synonym of C. sulphurascens. The circumscription of Phellinidium was delimited to accommodate Phellinidium asiaticum, Phellinidium ferrugineofuscum (the generic type), Phellinidium fragrans and Phellinidium pouzarii. Accordingly, the concept of Phellinidium was emended to accommodate resupinate species bearing cylindrical to oblong-ellipsoid or allantoid basidiospores. No species of Phellinidium under the new circumscription has been reported to be a forest pathogen. Phellinidium noxium and Phellinidium rufitinctum were excluded from Phellinidium, while the taxonomical positions of Phellinidium aciferum, Phellinidium lamaënse, and Phellinidium orientale are still uncertain.
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http://dx.doi.org/10.1016/j.funbio.2016.04.008DOI Listing
August 2016

Neomensularia duplicata gen. et sp. nov. (Hymenochaetales, Basidiomycota) and two new combinations.

Mycologia 2016 09 29;108(5):891-898. Epub 2016 Jul 29.

Institute of Microbiology, PO Box 61, Beijing Forestry University, Beijing 100083, China

Neomensularia (Hymenochaetaceae) is a new genus introduced for N. duplicata sp. nov. (generic type), N. crocitincta and N. kanehirae combs. nov., based on a combination of distinct morphological characters and phylogenetic analyses inferred from nuc rDNA partial 28S and ITS datasets. The new genus is characterized by annual, pileate, brown to fuscous basidiocarps, duplex context, dimitic hyphal structure, ventricose, and hooked hymenial setae, golden yellow, thick-walled, smooth basidiospores that are negative in both Melzer's reagent and Cotton Blue and a growth habit in tropical forests. Neomensularia duplicata closely resembles Mensularia radiata by hooked hymenial setae, but M. radiata has a homogenous context, larger pores (5-7 per mm), monomitic hyphal structure, hyphoid setae, larger cyanophilic basidiospores and occurs in temperate forests. Central American specimens labeled as Inonotus crocitinctus and Asian specimens identified as Fulvifomes kanehirae were re-examined. The duplex context, hooked hymenial setae and colored basidiospores are similar to N. duplicata Phylogenetic inferences based on 28S and ITS sequence data confirm their affinities with N. duplicata and result in the proposal of new combinations, Neomensularia crocitincta and N. kanehirae.
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http://dx.doi.org/10.3852/16-020DOI Listing
September 2016

Global diversity and phylogeny of the Phellinus igniarius complex (Hymenochaetales, Basidiomycota) with the description of five new species.

Mycologia 2016 Jan-Feb;108(1):192-204. Epub 2015 Nov 9.

State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, China

The Phellinus igniarius complex corresponds to Phellinus s.s., a well supported clade in the polyphyletic Phellinus s.l. studied worldwide. Recently a molecular phylogeny of this complex was performed for the European and North American species. In this study we expand the taxon samplings of the P. igniarius complex to include 59, seven and 12 additional collections originating from China, Czech Republic and USA, respectively. We generated 78 nuc rDNA ITS1-5.8S-ITS2 (ITS) and 42 translation elongation factor 1-α gene (tef1α) sequences. Based on the morphological and phylogenetic (combined ITS and tef1α dataset) analyses, Phellinus monticola, P. orientoasiaticus, P. padicola, P. parmastoi and P. pomaceoides are newly described and illustrated from China and USA. Phellinus pseudoigniarius is treated as a later synonym of P. igniarius, whereas Phellinus betulinus subsp. betulinus is accepted as P. betulinus. A total of 15 species are accepted in the P. igniarius complex worldwide. Of them, 10 species are distributed in eastern Asia, eight in Europe and six in North America. The taxonomy, phylogeny, host associations and geographic distributions of these 15 species are briefly discussed.
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http://dx.doi.org/10.3852/15-099DOI Listing
May 2016

Four new species of Phylloporia (Hymenochaetales, Basidiomycota) from tropical China with a key to Phylloporia species worldwide.

Authors:
Li-Wei Zhou

Mycologia 2015 Nov-Dec;107(6):1184-92. Epub 2015 Aug 21.

State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, People's Republic of China

Phylloporia (Hymenochaetaceae) is a monophyletic and cosmopolitan genus with diverse morphology. In a nuc 28S rDNA-based phylogeny specimens collected from tropical China were recovered as four new lineages within the Phylloporia clade. Based on morphological evidence and host relationships, these four lineages are described and illustrated as four new species: Phylloporia clausenae, P. cylindrispora, P. flacourtiae and P. homocarnica. The distinctive morphological characters of the four new species of Phylloporia are discussed. In addition, the phylogenetic position of P. tiliae, which was recently described from China, was confirmed in the Phylloporia clade. A key is provided for all 30 accepted species of Phylloporia.
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http://dx.doi.org/10.3852/14-254DOI Listing
March 2016

Electronic resistance switching in the Al/TiO(x)/Al structure for forming-free and area-scalable memory.

Nanoscale 2015 Jul;7(25):11063-74

School of Electronics Information Engineering, Tianjin Key Laboratory of Film Electronic & Communication Devices, Tianjin University of Technology, Tianjin 300384, China.

Electronic bipolar resistance switching (eBRS) in an Al/TiOx/Al structure, where the TiOx layer was reactively sputter-deposited, was examined in conjunction with a structural analysis using transmission electron microscopy. A thin (3-5 nm) insulating Al(Ti)Ox layer was formed at the bottom Al electrode interface, which provided the necessary asymmetric potential barrier for the eBRS to emerge, whereas the top Al electrode interface appeared to have provided the fluent carrier (electron) injection. The set and reset switching were related to the trapping and detrapping of the carriers at the trap centers, the characteristic energy of which was ∼0.86 eV, across the entire electrode area. The general features of this material system as the feasible RS memory were insufficient: endurance cycle, <∼8000, and retention time at 85 °C, 10(6) s. However, the detailed analysis of the switching behavior based on the space-charge limited current conduction mechanism, and its variation with the switching cycles, provided useful information on the general features of the eBRS, which could also be applicable to other binary (or even ternary) metal-oxide RS systems based on the electronic switching mechanism.
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http://dx.doi.org/10.1039/c4nr06417hDOI Listing
July 2015

A Novel Phellinidium sp. Causes Laminated Root Rot on Qilian Juniper (Sabina przewalskii) in Northwest China.

Plant Dis 2015 Jan;99(1):39-43

State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, China.

A laminated root rot on Qilian juniper (Sabina przewalskii) caused by a species of Phellinidium (Basidiomycota) was observed in northwest China. Seventeen fungal samples collected from Qinghai Province during 2012 and 2013 were used for taxonomic and pathogenicity tests. The fungal pathogen was identified by morphological characters and phylogenetic analyses based on nLSU sequences. A new fungus is described herein as Phellinidium qilianense sp. nov. One-year-old Qilian juniper seedlings were wound-inoculated under controlled conditions to test pathogenicity of the fungal species. The fungus was successfully reisolated from decayed tissue of tested seedlings. P. qilianense is a new forest pathogen on coniferous trees in China.
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http://dx.doi.org/10.1094/PDIS-03-14-0335-REDOI Listing
January 2015

Global diversity of the Ganoderma lucidum complex (Ganodermataceae, Polyporales) inferred from morphology and multilocus phylogeny.

Phytochemistry 2015 Jun 25;114:7-15. Epub 2014 Oct 25.

State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, China. Electronic address:

Species of the Ganoderma lucidum complex are used in many types of health products. However, the taxonomy of this complex has long been chaotic, thus limiting its uses. In the present study, 32 collections of the complex from Asia, Europe and North America were analyzed from both morphological and molecular phylogenetic perspectives. The combined dataset, including an outgroup, comprised 33 ITS, 24 tef1α, 24 rpb1 and 21 rpb2 sequences, of which 19 ITS, 20 tef1α, 20 rpb1 and 17 rpb2 sequences were newly generated. A total of 13 species of the complex were recovered in the multilocus phylogeny. These 13 species were not strongly supported as a single monophyletic lineage, and were further grouped into three lineages that cannot be defined by their geographic distributions. Clade A comprised Ganoderma curtisii, Ganoderma flexipes, Ganoderma lingzhi, Ganoderma multipileum, Ganoderma resinaceum, Ganoderma sessile, Ganoderma sichuanense and Ganoderma tropicum, Clade B comprised G. lucidum, Ganoderma oregonense and Ganoderma tsugae, and Clade C comprised Ganoderma boninense and Ganoderma zonatum. A dichotomous key to the 13 species is provided, and their key morphological characters from context, pores, cuticle cells and basidiospores are presented in a table. The taxonomic positions of these species are briefly discussed. Noteworthy, the epitypification of G. sichuanense is rejected.
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http://dx.doi.org/10.1016/j.phytochem.2014.09.023DOI Listing
June 2015

Colivelin ameliorates amyloid β peptide-induced impairments in spatial memory, synaptic plasticity, and calcium homeostasis in rats.

Hippocampus 2015 Mar 30;25(3):363-72. Epub 2014 Oct 30.

Department of Physiology, Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan, China.

Amyloid β peptide (Aβ) has been thought to be neurotoxic and responsible for the impairment of learning and memory in Alzheimer's disease (AD). Humanin (HN), a 24 amino acid polypeptide first identified from the unaffected occipital lobe of an AD patient, is believed to be neuroprotective against the AD-related neurotoxicity. In this study, we investigated the neuroprotective effects of Colivelin (CLN), a novel HN derivative, against Aβ by using behavioral test, in vivo electrophysiological recording, and intracellular calcium imaging. Our results showed that intrahippocampal injection of CLN (0.2 nmol) effectively prevented Aβ25-35 (4 nmol)-induced deficits in spatial learning and memory of rats in Morris water maze test; the suppression of in vivo hippocampal long term potentiation (LTP) by Aβ25-35 was nearly completely prevented by CLN; in addition, CLN pretreatment also effectively inhibited Aβ25-35-induced calcium overload in primary cultured hippocampal neurons. These results indicate that CLN has significant neuroprotective properties against Aβ, and CLN may holds great promise for the treatment and prevention of AD.
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http://dx.doi.org/10.1002/hipo.22378DOI Listing
March 2015

Phylogenetic analysis of ligninolytic peroxidases: preliminary insights into the alternation of white-rot and brown-rot fungi in their lineage.

Mycology 2014 Mar 25;5(1):29-42. Epub 2014 Mar 25.

State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, P. R. China.

White-rot and brown-rot fungi employ different mechanisms to degrade lignocellulose. These fungi are not monophyletic and even alternate in their common lineage. To explore the reason for this, seventy-six ligninolytic peroxidases (LPs), including 14 sequences newly identified from available basidiomycetous whole-genome and EST databases in this study, were utilized for phylogenetic and selective pressure analyses. We demonstrate that LPs were subjected to the mixed process of concerted and birth-and-death evolution. After the duplication events of original LPs, various LP types may originate from mutation events of several key residues driven by positive selection, which may change LP types and even rot types in a small fraction of wood-decaying fungi. Our findings provide preliminary insights into the cause for the alternation of the two fungal rot types within the same lineage.
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http://dx.doi.org/10.1080/21501203.2014.895784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979444PMC
March 2014

Phylogeny and taxonomy of poroid and lamellate genera in the Auriculariales (Basidiomycota).

Mycologia 2013 Sep-Oct;105(5):1219-30. Epub 2013 May 26.

State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, P.R. China.

The phylogeny of taxa with poroid and lamellate hymenophores in Auriculariales as yet has not been well studied. Here we sequenced the nLSU and ITS regions of poroid and lamellate representatives from the genera Elmerina, Protodaedalea and Protomerulius to establish the phylogenetic position of these morphologically similar taxa. Our analyses suggest that (i) Elmerina foliacea is divergent from all other Elmerina species sampled; (ii) Protodaedalea hispida (the type of monotypic genus Protodaedalea) and Protomerulius efibulatus should be transferred to Elmerina as E. hispida and E. efibulata respectively; (iii) after exclusion of P. efibulatus, Protomerulius becomes monophyletic and represents the core of a "Protomerulius family" clade that potentially includes species from Tremellodendropsis, Heterochaetella and Protodontia; and (iv) the presence or absence of gloeocystidia serves as the most reliable morphological feature in delimiting Elmerina s.l. and Protomerulius. Concepts of Elmerina s.l. and Protomerulius are redefined, and diagnostic keys for these two genera are provided.
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http://dx.doi.org/10.3852/12-212DOI Listing
June 2014

Taxonomy and phylogeny of wood-inhabiting hydnoid species in Russulales: two new genera, three new species and two new combinations.

Mycologia 2013 May-Jun;105(3):636-49. Epub 2013 Jan 29.

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, People's Republic of China.

Russulales comprises a highly diverse group of species with respect to basidiocarp morphology and hymenophore type. We reconstructed evolutionary histories of Russulales using ITS and nLSU rDNA sequence data with an emphasis on phylogeny of wood-inhabiting hydnoid species in this order. Based on the combination of morphological and molecular evidence, two new genera, three new species and two new combinations are proposed. Dentipellis microspora and D. coniferarum, newly described, were placed outside Hericiaceae but are provisionally treated in Dentipellis due to their morphological similarity to the generic type. Dentipellis leptodon and D. taiwaniana are transferred to the new genus Dentipellicula as D. leptodon and D. taiwaniana, which is designated as the generic type. The new genus Dentipellopsis was erected to accommodate a newly described species D. dacrydicola. Characters are provided in a generic key to distinguish Dentipellicula, Dentipellis and Dentipellopsis that morphologically are highly similar in addition to a key to the current species in Dentipellis. A new species of Gloeodontia was described based on both molecular and morphological data, and a key to this genus also is provided.
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http://dx.doi.org/10.3852/12-011DOI Listing
July 2013

Phylogeny and taxonomy of the recently proposed genus Phellinopsis (Hymenochaetales, Basidiomycota).

Mycologia 2013 May-Jun;105(3):689-96. Epub 2012 Dec 11.

Chinese Academy of Sciences, Shenyang, People's Republic of China.

Phellinopsis, a recently proposed genus, differs from other genera in Hymenochaetaceae in having setae arising from tramal hyphae and hyaline basidiospores that become pale yellowish with age. The monophyly of 11 taxa, including seven from China, was strongly supported in our phylogeny of ITS sequences. Two species, Phellinopsis junipericola and P. resupinata, are newly described and illustrated from Chinese specimens based on morphological and phylogenetic evidence, bringing the number of known Phellinopsis species worldwide to four. Phylogeny and genetics showed that the North American species, Phellinopsis occidentalis, had an earlier divergence from the other three species. Phellinopsis conchata is distributed in China, Europe and North America, with the Chinese and North American specimens having a closer relationship. An identification key to the four species of Phellinopsis is provided.
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http://dx.doi.org/10.3852/12-145DOI Listing
July 2013

Wood-inhabiting fungi in southern China 5. New species of Theleporus and Grammothele (Polyporales, Basidiomycota).

Mycologia 2012 Jul-Aug;104(4):915-24. Epub 2012 Feb 7.

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, People's Republic of China.

During the examination of specimens of Theleporus and Grammothele (Polyporales, Basidiomycota) from tropical China, three new species, Theleporus membranaceus, T. minisporus and Grammothele denticulata, were identified based on both morphological and phylogenetic analyses. They are described and illustrated. T. membranaceus is characterized by its extremely thin basidiocarps (0.12 mm), small pores (7-10 per mm) and ellipsoid to broadly ellipsoid basidiospores. T. minisporus has the smallest basidiospores among the species in the genus. Grammothele denticulata is distinguished in the genus by gray pores, continuous hymenia over dissepiment edge and cylindrical basidiospores with tapering apex. Two annotated identification keys are provided for species thus far accepted in Theleporus and Grammothele. The phylogenetic relationships of Theleporus and Grammothele were inferred based on nITS sequences and are briefly discussed. The molecular evidence showed that Theleporus and Grammothele belong to the core polyporoid clade.
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http://dx.doi.org/10.3852/11-302DOI Listing
September 2012

Phylogeny and taxonomy of Phylloporia (Hymenochaetales): new species and a worldwide key to the genus.

Mycologia 2012 Jan-Feb;104(1):211-22. Epub 2011 Sep 20.

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, People's Republic of China.

Species of Phylloporia (Hymenochaetales, Basidiomycota) are polypores with tiny, yellowish and thick-walled basidiospores and are highly divergent in morphology. The monophyly of 35 cosmopolitan isolates of Phylloporia was strongly supported based on analysis of the nLSU rDNA gene but with exclusion of P. resupinata. Among the 19 lineages recognized in the nLSU rDNA phylogeny five new species from China, Phylloporia crataegi, P. fontanesiae, P. gutta, P. nandinae and P. oreophila, were revealed and these species are introduced with additional morphological evidence. A diagnostic key to all 23 thus far accepted species in Phylloporia is provided.
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http://dx.doi.org/10.3852/11-093DOI Listing
May 2012

Current advances in Phellinus sensu lato: medicinal species, functions, metabolites and mechanisms.

Appl Microbiol Biotechnol 2010 Aug 12;87(5):1587-93. Epub 2010 Jun 12.

Institute of Microbiology, Beijing Forestry University, PO Box 61, Beijing 100083, China.

Twenty-six species of Phellinus sensu lato, reported as medicinal mushrooms, are enumerated in this review. The species' names were checked and revised according to contemporary taxonomy and the latest version of the International Code of Botanical Nomenclature (Vienna Code); two misapplied names of Phellinus baumii Pilát and Phellinus himalayensis Y.C. Dai in previous reports are also discussed. Of the 20 types of medicinal functions, the most shared functions are antitumor and improving immunity, both of which may be viewed as the basal functions of Phellinus s. l. In addition, alleviating septic shock, anti-inflammatory, and antioxidation are also a routine functions mentioned often. The main medicinal metabolites, including several kinds of polysaccharides and polyphenols, are introduced. Different methods and conditions could purify various polysaccharides with difference in activity level even from the same species, while all polyphenols are hispidin and its derivatives in general. Three aspects of mechanism contribute to antitumor activities of polysaccharides: (1) promoting an immune response, (2) inducing cell apoptosis, and (3) inhibiting metastasis. Other general mechanisms of the metabolites in antioxidant activity, and in treating diabetes, as well as complications are summarized. We also elaborate on potential scientific strategies for obtaining the medicinal metabolites from Phellinus s. l., such as artificial cultivation, the discoveries of more species with medicinal functions, the utilization of species growing quickly, and the optimization of culture conditions and media supplements in fermentation.
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http://dx.doi.org/10.1007/s00253-010-2711-3DOI Listing
August 2010

[Progress in the study on alternative splicing and functions of kininogen genes].

Yi Chuan 2006 Dec;28(12):1649-55

Institute of Functional Gene & Proteomics of Marine Biology, Liaoning Normal University, Dalian 116029, China.

The kininogen gene and its coded proteins are slightly different in various species. In human beings, bovine and mouse, for example, there is an alternatively spliced kininogen gene K, encoding two kinds of proteins. By contrast, there is still another constituted spliced kininogen gene T which encodes only one kind of protein in the rat. The kininogen, belonging to the family 3 of cystatin superfamily, is a kind of multifunctional proteins with multiple domains, which maintains the normal physiological condition in human and some other organisms. The antagonism of hemoglutination and antihemoglutination of kininogen can not only recuperate the damaged blood vessels to prevent them from bleeding ceaselessly, but also restrain the formation of thrombus. In this review, we briefly discussed alternative splicing of kininogen gene and the multifunction of kininogen protein, as well as primarily hemoglutination and antihemoglutination, in human beings, bovine, mouse and rat which have been currently studied in detail. Our aims are to provide the beneficial references for further understanding the mechanism of evolution and alternative splicing of kininogen gene, and elucidating the multifunction roles of kininogen protein. Besides, it would be helpful for developing new medicines to regulate the vascular permeability and blood pressure and to restrain tumor.
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http://dx.doi.org/10.1360/yc-006-1649DOI Listing
December 2006

[Report of a rare distal arthrogrophy large family].

Yi Chuan 2004 Nov;26(6):803-6

Liaoning Open Laboratory of Reproductive Medicine, Shenyang 110031,China.

We describe a seven-generation large family with talipomanus and talipes, 175 individuals in this family were involved. 32 affected individuals including 18 males and 14 females whose clinical features were different and 1 suspicious male were found. The talipomanus were symmetrical, and varus and valgus were caused by vertical talus. We investigated their living environment, the dietary habit, obstetrical history, physical status, lifespan, and studied cytogenetics and so on. We propose these defects were rare distal arthrogrophy genetic disease.
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November 2004

[Experimental study on the reconstruction of mandibular defects with a new bioactive artificial bone nano-hydroxyapatite/polyamide-66 in dogs].

Zhonghua Kou Qiang Yi Xue Za Zhi 2004 Jan;39(1):60-2

Department of Oral Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu 610041, China.

Objective: This study was designed to investigate reconstruction of segmental defect in the mandible using a new bionic materials of nano-hydroxyapatite -polyainides-66 (n-HA/PA66).

Methods: Two defects (15 mm x 10 mm x 5 mm) were created in the mandibular bodies of dogs. One of defects was reconstructed with n-HA/PA66, another not repaired as a blank control. At 2, 4, 8, 12, 16 weeks after operation. Evaluation of effects of n-HA/PA66 on reconstruction of the mandibular defects was carried out by means of radiography and histology.

Results: From 2 to 8 weeks after operation, some fiber tissue grew into the space between n-HA/PA66 and mandibular bone. The ossification was observed at 12 weeks post-operation. At 16 weeks, the n-HA/PA66 was connected directly to the mandibular bone by the newborn bone.

Conclusions: The new artificial bone of n-HA/PA66 has the effects of osteoconduction and osteoinduction, with a good biocompatibility and is an ideal bone substitute material for reconstruction of mandibular defect.
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January 2004