Publications by authors named "Li-Jun Chen"

191 Publications

[Development and clinical application of universal screwdriver].

Zhongguo Gu Shang 2021 Jul;34(7):622-7

Department of Orthopaedics, Chinaco Healthcare Corporation Limited International Hospital, Ningbo 315315, Zhejiang, China.

Objective: To develop a universal screwdriver for sealing the end of the central hole of the femoral interlocking intramedullary nail, so as to shorten the operation time of the tail cap implantation of the intramedullary nail and improve the accuracy of implantation.

Methods: Total 77 patients with intertrochanteric fractures underwent femoral interlocking intramedullary nail (FIIN) surgery from June 2018 to June 2019. There were 28 males and 49 females, aged 55 to 80 (76.22± 7.32) years old, and course of disease was 20 to 40 h. All patients were divided into universal screwdriver group (39 cases) and ordinary screwdriver group (38 cases) according to whether the self-developed universal screw was applicable during the operation. The blood loss during tail cap implantation, the time of tail cap implantation, the success rate of one-time implantation, and the postoperative curative effect were compared between two groups.

Results: All patients were followed up for 12 to 36 months, with an average of(20.00±6.38) months. The bleeding volume and the time of tail cap implantation in the universal screwdriver group were significantly lower thanthose in the ordinary screwdriver group (<0.05);The one-time success rate (100%) of the universal screwdriver group was significantly higher than that of the ordinary screwdriver group (75.4%)(< 0.05);at the last follow-up, the efficacy scores of two groups were improved compared with preoperatively, but the efficacy scores of the universal screwdriver group were significantly better than those of the ordinary screwdriver group(<0.05).

Conclusion: The universal screwdriver is easy to operate during the operation when using the cap of the femoral intramedullary nail, the operation time is shortened, the amount of bleeding is reduced, and the treatment effect is satisfactory.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2021.07.007DOI Listing
July 2021

Postoperative evaluation of left ventricular global strain using cardiac computed tomography in pediatric patients with congenital heart disease: A comparison with echocardiography.

Eur J Radiol 2021 Jul 21;142:109868. Epub 2021 Jul 21.

Diagnostic Imaging Center, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, 1678 Dong Fang Road, Shanghai 200127, PR China. Electronic address:

Purpose: We explored the feasibility and reproducibility of cardiac computed tomography (CCT)-derived left ventricular (LV) global strain in postoperative children
with congenital heart disease (CHD) and compared its correlation and agreement with transthoracic echocardiography (TTE).

Methods: Fifty-one patients (28 males, 23 females) were included who underwent clinically indicated retrospective electrocardiography-triggered CCT. and all patients underwent additional TTE on the same day. LV global longitudinal strain (GLS), global circumferential strain (GCS), and global radial strain (GRS) were measured. Correlations of global strains between CCT and TTE were assessed using Pearson's correlation coefficient. Intra-class correlation coefficients (ICC) were used to assess CCT intra-observer and inter-observer reproducibility.

Results: GLS and GCS were not significantly different between CCT and TTE (GLS: -23.54 ± 3.24 vs. -23.85 ± 3.72, respectively, p = 0.415; GCS: -28.21 ± 3.55 vs. -28.79 ± 3.69, respectively, p = 0.155). GRS was significantly different between CCT and TTE (60.79 ± 15.11 vs. 41.73 ± 4.27, respectively, p < 0.001). There was good correlation between CCT- and TTE-derived GLS (r = 0.70, p < 0.001) and GCS (r = 0.68, p < 0.001), but GRS showed no correlation between CCT and TTE (r = 0.09, p = 0.54). CCT-derived global strain showed good intra- and inter-observer reproducibility (ICC = 0.86-0.92), except the inter-observer reproducibility for GRS (ICC = 0.77).

Conclusions: CCT was feasible for postoperative evaluation of LV global strain in pediatric patients with CHD with sufficient reproducibility. CCT-derived global strain can provide additional information in selected CHD patients with poor acoustic windows and who are intolerant to or have contraindications for cardiac magnetic resonance.
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http://dx.doi.org/10.1016/j.ejrad.2021.109868DOI Listing
July 2021

Treatment of primary cardiac angiosarcoma in a 35 weeks pregnant woman.

J Card Surg 2021 Jul 3. Epub 2021 Jul 3.

Department of Cardiac Surgery, People's Hospital of Dongyang, Dongyang, Zhejiang, China.

Background: Cardiac angiosarcoma is a rare but highly malignant cardiac tumor. It is characterized by poor prognosis, and current treatment approaches are not effective.

Case Presentation: A 37-year-old female with 35 weeks pregnancy experienced chest tightness and shortness of breath for 1 month. She was diagnosed with primary cardiac angiosarcoma. Delivery of fetus was performed early to treat the mother. The patient underwent resection of the tumor then she was treated with chemotherapy. However, the tumor recurred 11 months after surgery.

Conclusion: Angiosarcoma is a highly malignant tumor explaining recurrence of the tumor recurred after surgery. Cardiac angiosarcoma should be treated through a comprehensive treatment plan, comprising surgery, radiotherapy, and chemotherapy approaches.
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http://dx.doi.org/10.1111/jocs.15751DOI Listing
July 2021

Ventricular function and dyssynchrony in children with a functional single right ventricle using real time three-dimensional echocardiography after fontan operation.

Echocardiography 2021 Jun 22. Epub 2021 Jun 22.

Department of Pediatric Cardiology, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: This study aimed to evaluate the feasibility and clinical value of real time three-dimensional echocardiography (RT-3DE) for assessing ventricular systolic dysfunction and dyssynchrony in children with an functional single right ventricle (FSRV) having undergone the Fontan procedure.

Methods: Twenty-five children with an FSRV and 25 healthy children were enrolled in our study. RV volume analysis was performed compared with magnetic resonance imaging (MRI) as the reference standard in FSRV patients. The patients were divided into wide and narrow QRS interval groups. Global and regional functions of the RV in three compartments (inflow, body, and outflow) were compared between FSRV and control subjects, including RV systolic dyssynchrony indices of maximal difference of time to minimal volume (Tmsv-Dif), standard deviation of time to minimal volume (Tmsv-SD), maximal difference of time to minimal volume corrected by R-R interval (Tmsv-Dif%), and standard deviation of time to minimal volume corrected by R-R interval (Tmsv-SD%).

Results: RT-3DE measurements were significantly lower than MRI measurements for RV-EDV, RV-ESV, RV-SV, and RVEF (p < 0.01).Compared with controls, patients with an FRSV had significantly higher dyssynchrony indices and significantly lower global EF in both narrow QRS interval and wide QRS interval groups. Tmsv-SD% was shown to be most strongly correlated with MRI-RVEF (r = -.570, p = 0.003).

Conclusions: RT-3DE tended to underestimate RV ventricular volume in children with FSRV. Children with an FSRV and either a wide or narrow QRS interval had reduced ventricular function and higher dyssynchrony than normal subjects. Worsening RV dyssynchrony is associated with overall decline in function after the Fontan operation.
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http://dx.doi.org/10.1111/echo.15148DOI Listing
June 2021

A Two-Dimensional Metallacycle Cross-Linked Switchable Polymer for Fast and Highly Efficient Phosphorylated Peptide Enrichment.

J Am Chem Soc 2021 Jun 27;143(22):8295-8304. Epub 2021 May 27.

School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia.

The selective and efficient capture of phosphopeptides is critical for comprehensive and in-depth phosphoproteome analysis. Here we report a new switchable two-dimensional (2D) supramolecular polymer that serves as an ideal platform for the enrichment of phosphopeptides. A well-defined, positively charged metallacycle incorporated into the polymer endows the resultant polymer with a high affinity for phosphopeptides. Importantly, the stimuli-responsive nature of the polymer facilitates switchable binding affinity of phosphopeptides, thus resulting in an excellent performance in phosphopeptide enrichment and separation from model proteins. The polymer has a high enrichment capacity (165 mg/g) and detection sensitivity (2 fmol), high enrichment recovery (88%), excellent specificity, and rapid enrichment and separation properties. Additionally, we have demonstrated the capture of phosphopeptides from the tryptic digest of real biosamples, thus illustrating the potential of this polymeric material in phosphoproteomic studies.
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http://dx.doi.org/10.1021/jacs.0c12904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193630PMC
June 2021

Molecular response uncovers neurotoxicity of Pardosa pseudoannulata exposed to cadmium pressure.

Environ Pollut 2021 Jul 22;280:117000. Epub 2021 Mar 22.

Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA.

Cadmium (Cd) is a widely distributed heavy metal in south of China. Growing evidence indicates that systemic exposure to Cd, particularly the long-term exposure, may cause neurotoxic effects. Nevertheless, mechanisms underlying Cd neurotoxicity remain not completely understood. In this report, we investigated the neural alterations in the spider Pardosa pseudoannulata (Bösenberg and Strand, 1906) exposed to long-term Cd (LCd) and short-term Cd (SCd) pressure. Cd stress lowered foraging ability and prey consuming time in the spiders. In addition, enzymatic analysis results indicated that Cd exposure reduced the level of acetylcholinesterase at subcellular level. We then identified differentially expressed genes (DEGs) in the Cd exposed spiders using pairwise comparisons and found that a large number of DEGs were related to neurotransmitter receptors and ion transport and binding proteins. Notably, LCd exposure harbored more altered genes in ion transporter activity comparing with SCd exposure. From six K-means clusters, 53 putative transcriptional factors (TFs) belonging to 21 families were characterized, and ZBTB subfamily displayed the most distinctive alterations in the characterized genes, which is assumed to play a key role in the regulation of ion transmembrane process under Cd stress. A protein-to-protein interaction network constructed by the yielded DEGs also showed that ion and receptor binding activities were affected under long-term Cd exposure. Four key modules from the network indicated that Cd may further down-regulate energy metabolism pathway in spiders. Collectively, this comprehensive analysis provides multi-dimensional insights to understand the molecular response of spiders to Cd exposure.
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http://dx.doi.org/10.1016/j.envpol.2021.117000DOI Listing
July 2021

The relationship among social capital, eHealth literacy and health behaviours in Chinese elderly people: a cross-sectional study.

BMC Public Health 2021 01 6;21(1):45. Epub 2021 Jan 6.

School of Acupuncture and Tuina, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China.

Background: Social capital has been linked to health behaviours, but the underlying mechanism is unclear. Previous studies have found that health literacy played the role of a mediator in the relationships among social capital, individual physical activity and nutrition. But it is not clear whether eHealth literacy mediates the impact of social capital on health behaviours. Therefore, our research aimed to explore the relationships among social capital (structural and cognitive social capital), eHealth literacy, and the health behaviours of elderly people, and to analyse the mediating effect of eHealth literacy, while providing a theoretical basis for a health behaviour intervention for elderly people.

Methods: From January to February 2019, we conducted a cross-sectional survey of 1201 Chinese people aged over 60 years using the Chinese Shortened Social Capital Scale (contains two subscales of structural social capital and cognitive social capital), eHealth Literacy Scale, and Health-Promoting Lifestyle Profile. We used structural equation modelling to test a hypothetical mediation model.

Results: The mean scores of social capital was 72.07 (SD = 13.03), 17.24 (SD = 9.34) for eHealth literacy, and 112.23 (SD = 23.25) for health behaviours. Social capital and eHealth literacy were significantly correlated with health behaviours, and social capital and structural social capital were significantly correlated with eHealth literacy. Lastly, eHealth literacy mediated the relationship between structural social capital and health behaviours.

Conclusions: eHealth literacy was an important mediating factor for elderly people's structural social capital and health behaviours. Therefore, social capital and eHealth literacy must be considered when designing and implementing health behaviour intervention programmes for elderly people.
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http://dx.doi.org/10.1186/s12889-020-10037-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789238PMC
January 2021

Diagnosis of Double-chambered Left Ventricle of Superior-inferior Type by Echocardiography: A Retrospective Study of 9 Subjects across Two Heart Centers.

Echocardiography 2021 02 24;38(2):169-174. Epub 2020 Dec 24.

Department of Radiology, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Background: Double-chambered left ventricle (DCLV) is extremely rare. Challenges remain in accurate diagnosis of DCLV by echocardiography, because it is easily confused with left ventricular diverticulum, left ventricular aneurysm, ventricular septal defect, etc. Herein, we reviewed echocardiographic characteristics of DCLV and evaluated the accuracy of echocardiographic diagnosis.

Methods: Clinical and echocardiographical databases of two heart centers were reviewed. Nine children with DCLV of superior-inferior arrangement were studied retrospectively.

Results: Normal elliptical geometry of LV cavity is preserved in DCLV. Fibromuscluar bundles separate LV into the main chamber and the accessory chamber. The associated malformations included ventricular septal defects, mitral regurgitation, mitral valve stenosis, pulmonary stenosis, and left ventricular noncompaction in our study. Eight of nine subjects with DCLV of superior-inferior arrangement were diagnosed correctly by initial echocardiographic examination, and one case was mistaken as left ventricular diverticulum.

Conclusions: Double-chambered left ventricle could be diagnosed and followed up by echocardiography. The morphological classification is a simplified way to improve diagnostic accuracy.
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http://dx.doi.org/10.1111/echo.14939DOI Listing
February 2021

[Effect of nitrogen additions on soil pH, phosphorus contents and phosphatase activities in grassland].

Ying Yong Sheng Tai Xue Bao 2020 Sep;31(9):2985-2992

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.

Phosphorus is a key nutrient for all plant species and a limiting factor for grassland ecosystem function. In recent years, in response to the rapid increase of global nitrogen deposition, soil phosphorus contents and phosphatase activities changed to varying degrees in grassland ecosystems. We conducted a meta-analysis to examine the responses of soil pH, total phosphorus (TP), available phosphorus (AP), as well as activities of alkaline phosphatase (AlP) and acid phosphatase (AcP) in soils to nitrogen addition amount, nitrogen type, experimental duration, and sampling depth. The correlation between soil pH and phosphatase response ratio was investigated. The results showed that nitrogen addition significantly reduced soil pH, TP and AlP activity, while significantly increased AcP activity, but had no significant effect on AP. Soil pH and AlP activity significantly decreased under nitrogen addition >5 g·m·a, and AcP activity significantly increased under high nitrogen addition (>10 g·m·a). The contents of TP and AP significantly decreased when nitrogen addition was 5-10 g·m·a. NHNO treatment significantly reduced soil TP and increased AcP activity, while urea treatment significantly reduced soil pH and AlP activity. Across all nitrogen addition amounts, when the experiment duration was 3 to 10 years, soil TP content and AlP activity were significantly reduced. Soil pH was significantly reduced after three years nitrogen addition, and AcP activitiy was significantly increased after 10 years nitrogen addition. In the 0-10 cm soil layer, the TP content and AlP activity significantly decreased, while the AP content significantly increased. In >10 cm soil layer, the AP content was significantly decreased. The significant negative correlation between soil pH and AcP activity indicated that change in soil pH caused by nitrogen addition may be an important factor for the variation of soil phosphatase activity.
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http://dx.doi.org/10.13287/j.1001-9332.202009.034DOI Listing
September 2020

[Effects of freeze-thaw on dissolved nitrogen pool, nitrogen transformation processes and diversity of bacterial community in temperate soils].

Ying Yong Sheng Tai Xue Bao 2020 Sep;31(9):2893-2902

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.

Soil freeze-thaw could affect nitrogen (N) availability. The N transformation is closely related with soil microbes. The effect of soil freeze-thaw on the soil bacterial communities in the temperate zone is still not clear. We hypothesized that freeze-thaw events could affect the diversity and composition of bacterial communities, thereby changing the contents of soil dissolved nitrogen pools as well as the N transformation process. In this study, microcosms with different freeze-thaw cycles (six and fifteen cycles) were designed, with the constant temperature at 2 ℃ as the control. The results showed that the contents of dissolved total nitrogen, dissolved inorganic nitrogen, microbial biomass nitrogen and net nitrogen mineralization rate were decreased significantly in response to increasing cycles of freeze-thaw. The number of freeze-thaw cycles did not affect bacterial α diversity. In contrast, the duration of incubation was positively correlated with bacterial α diversity including Chao1 and Shannon indices. Freeze-thaw treatment significantly affected the function and composition of bacterial communities, but the number of freeze-thaw cycles had little effect on the bacterial community structure. The partial redundant analysis showed that under freeze-thaw treatments, both the composition and function of bacterial community were significantly related to soil dissolved N pools and N transformation processes.
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http://dx.doi.org/10.13287/j.1001-9332.202009.033DOI Listing
September 2020

Multifunctional role of microRNAs in mesenchymal stem cell-derived exosomes in treatment of diseases.

World J Stem Cells 2020 Nov;12(11):1276-1294

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.

Mesenchymal stem cells can be replaced by exosomes for the treatment of inflammatory diseases, injury repair, degenerative diseases, and tumors. Exosomes are small vesicles rich in a variety of nucleic acids [including messenger RNA, Long non-coding RNA, microRNA (miRNA), and circular RNA], proteins, and lipids. Exosomes can be secreted by most cells in the human body and are known to play a key role in the communication of information and material transport between cells. Like exosomes, miRNAs were neglected before their role in various activities of organisms was discovered. Several studies have confirmed that miRNAs play a vital role within exosomes. This review focuses on the specific role of miRNAs in MSC-derived exosomes (MSC-exosomes) and the methods commonly used by researchers to study miRNAs in exosomes. Taken together, miRNAs from MSC-exosomes display immense potential and practical value, both in basic medicine and future clinical applications, in treating several diseases.
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http://dx.doi.org/10.4252/wjsc.v12.i11.1276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705472PMC
November 2020

Microbial diversity and community structure changes in the rhizosphere soils of from different planting years.

Plant Signal Behav 2021 02 8;16(2):1854507. Epub 2020 Dec 8.

National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, the Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, the Ministry of Education, College of Life Sciences, Shaanxi Normal University , Xi'an, The People's Republic of China.

is a type of typical traditional Chinese medicinal (TCM) herb that is economically important in China. The traditional planting method of is to plant continuously for many years, which often leads to impediments for its growth and development and soil-borne diseases. The root-associated microbiome is believed to play an important role in plant resistance and the quality of products from the plant. This study aims to reveal detailed changes in the populations of rhizosphere microorganisms, and providing theoretical guidance for the prevention and control of soil-borne diseases in . A high-throughput sequencing approach was utilized to illustrate changes in the microbial community from different planting years. Results and conclusions: The results show that the diversity and composition of the root-associated microbiome was significantly impacted by the consecutive monoculture of . At the level of the comparisons of the phyla, Bacteroidetes, Proteobacteria, Ascomycota, and Basidiomycota declined significantly. In contrast, the relative abundance of Actinobacteria, Acidobacteria, and Mortierellomycota distinctly increased. Comparisons at the genus level indicated that , and decreased significantly, whereas , and distinctly increased. In conclusion, this study helps to develop an understanding of the impediments involved in the consecutive monoculture of .
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http://dx.doi.org/10.1080/15592324.2020.1854507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849755PMC
February 2021

Diagnosis value preoperative localization of insulinoma by diffusion-weighted imaging: A pilot study.

Medicine (Baltimore) 2020 Dec;99(49):e23048

The First Affiliated Hospital of Xi'an Jiao Tong University, Xi'an, ShannXi, China.

Insulinoma is the most common functional neuroendocrine tumor that originates from the islet of beta cells. Insulinoma is usually an isolated benign tumor and small in size (<2 cm). Due to the small size of the lesion, it often leads to difficulty in clinical preoperative localization diagnosis. However, we have unexpectedly discovered that the diffusion-weighted-imaging (DWI) adds great value in the preoperative localization diagnosis of insulinoma in non-invasive examination technique.We verified using operative pathology data and retrospectively analyzed the clinical and imageology findings of 5 cases who reported to have an insulinoma. All the 5 cases underwent DWI examination, among non-contrast enhanced magnetic resonance imaging (MRI) in 1 case, contrast-enhanced MRI in 4 cases.Five cases of DWI showed a nodular high signal <1.3 cm with pancreatic tail in 3 cases, pancreatic neck, and pancreatic head in 1 case each, respectively. Non-contrast enhanced MRI showed suspicious abnormal signals in the tail of the pancreas were detected in 1 case. MRI enhanced scans presented 2 cases with abnormal enhancement in the arterial phase and 2 cases without abnormal enhancement in arterial phase. Also, 3 cases showed mild persistence enhanced in the portal venous phase and delayed phase. However, 1 case remained normal in the portal venous phase and the delay period.DWI examination has high clinical value in the localization diagnosis of insulinoma and thus it can be used as a routine examination for preoperative localization diagnosis.
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http://dx.doi.org/10.1097/MD.0000000000023048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7717785PMC
December 2020

Assessment of migration regularity of phthalates from food packaging materials.

Food Sci Nutr 2020 Oct 12;8(10):5738-5747. Epub 2020 Sep 12.

Tianjin Key Laboratory of Food Science and Health School of Medicine Nankai University Tianjin China.

Phthalate acid esters (PAEs) are one of the essential plastic additives which may lead to plenty of harmful effects, including reproductive toxicity, teratogenicity, and carcinogenicity. Increasing attention has been paid to the migration of plasticizer. In this article, the disposable plastic lunch boxes were taken as the research object. The result showed that dibutyl phthalate (DBP) and diisobutyl phthalate (DIBP) have been mainly found, whose content was 1.5 mg/kg and 2.4 mg/kg, respectively. The LOD was 2 ng/g, and LOQ was 6.7 ng/g. We further investigated the migration of PAEs into the simulated liquid at different temperature conditions. Then, the linear fitting performing by first-order kinetic migration model revealed that the lower the polarity of the simulated liquid, the larger the rate constant and initial release rate . The higher the temperature, the bigger the and .
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http://dx.doi.org/10.1002/fsn3.1863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590312PMC
October 2020

Comparison of UV/HO, UV/PMS, and UV/PDS in Destruction of Different Reactivity Compounds and Formation of Bromate and Chlorate.

Front Chem 2020 25;8:581198. Epub 2020 Sep 25.

School of Water Conservancy and Civil Engineering, Northeast Agricultural University, Harbin, China.

In this study, we compared the decontamination kinetics of various target compounds and the oxidation by-products (bromate and chlorate) of PMS, PDS, and HO under UV irradiation (UV/PMS, UV/PDS, UV/HO). Probes of different reactivity with hydroxyl and sulfate radicals, such as benzoic acid (BA), nitrobenzene (NB), and trichloromethane (TCM), were selected to compare the decontamination efficiency of the three oxidation systems. Experiments were performed under acidic, neutral, and alkaline pH conditions to obtain a full-scale comparison of UV/peroxides. Furthermore, the decontamination efficiency was also compared in the presence of common radical scavengers in water bodies [bicarbonate, carbonate, and natural organic matter (NOM)]. Finally, the formation of oxidation by-products, bromate, and chlorate, was also monitored in comparison in pure water and tap water. Results showed that UV/HO showed higher decontamination efficiency than UV/PDS and UV/PMS for BA degradation while UV/HO and UV/PMS showed better decontamination performance than UV/PDS for NB degradation under acidic and neutral conditions. UV/PMS was the most efficient among the three processes for BA and NB degradation under alkaline conditions, while UV/PDS was the most efficient for TCM degradation under all pH conditions. In pure water, both bromate and chlorate were formed in UV/PDS, small amounts of bromate and rare chlorate were observed in UV/PMS, and no detectable bromate and chlorate were formed in UV/HO In tap water, no bromate and chlorate were detectable for all three systems.
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http://dx.doi.org/10.3389/fchem.2020.581198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545204PMC
September 2020

Polysaccharide Inhibited Hypoxia-Inducible Factor 1 in COPD Patients.

Int J Chron Obstruct Pulmon Dis 2020 24;15:1997-2004. Epub 2020 Aug 24.

Department of Clinical Institute, Ningxia Medical University, Yinchuan, Ningxia, People's Republic of China.

Background: Chronic obstructive pulmonary disease (COPD) is a chronic airway inflammatory disease characterized by irreversible airflow obstruction. Pathogenic mechanisms underlying COPD remain largely unknown.

Objective: The current study was designed to explore serum concentration of hypoxia-inducible factor 1α (HIF-1α) in stable COPD patients and the potential effect of polysaccharides (LBP) on HIF-1α protein expression.

Methods: Serum HIF-1α was quantified by ELISA in 102 stable COPD patients before and after 2-week orally taken LBP (100 mL/time, twice daily, 5-15 mg/mL). Correlation of serum LBP and lung function (FEV1%) or blood gas (PO and PCO) was also analyzed. As a control, 105 healthy subjects were also enrolled into this study.

Results: Serum concentration of HIF-1α was significantly higher in the stable COPD patients (37.34 ± 7.20 pg/mL) than that in the healthy subjects (29.55 ± 9.66 pg/mL, <0.001). Oral administration of LBP (5 mg/mL, 100 mL, twice daily for 2 weeks) not only relieved COPD symptoms but also significantly reduced serum HIF-1α concentration (36.94 ± 9.23 vs 30.49 ± 6.42 pg/mL, <0.05). In addition, level of serum HIF-1α concentration was significantly correlated with PCO (r = 0.283, <0.001), but negatively and significantly correlated with PO (r = -0.490, =0.005) or FEV1%(r = -0.420, =0.018).

Conclusion: These findings suggested that activation of HIF-1 signaling pathway may be involved in the pathophysiology of COPD and that stabilization of serum HIF-1α concentration by LBP might benefit the stable COPD patients.
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http://dx.doi.org/10.2147/COPD.S254172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7455768PMC
June 2021

A novel identified circular RNA, circ_0000491, aggravates the extracellular matrix of diabetic nephropathy glomerular mesangial cells through suppressing miR‑101b by targeting TGFβRI.

Mol Med Rep 2020 Nov 2;22(5):3785-3794. Epub 2020 Sep 2.

Department of Endocrinology, Zhejiang Integrated and Western Medicine Hospital, Hangzhou, Zhejiang 310003, P.R. China.

Circular RNAs (circRNAs) have crucial roles in various diseases; however, the mechanisms of action underlying circRNAs in the occurrence and development of diabetic nephropathy (DN) remains largely unknown. The present study investigated the differentially expressed circRNAs in the DN mice kidney cortex using circRNA sequencing and elucidated the role of circRNAs in mesangial cells. It was revealed that 40 circRNAs were unconventionally expressed, including 18 upregulated circRNAs and 22 downregulated circRNAs. Furthermore, circ_0000491 levels were significantly augmented in both DN mice and high glucose (HG, 30 mM)‑induced mouse mesangial cells (MES13 cells). Knockdown of circ_0000491 significantly suppressed the increase of vimentin, fibronectin and α‑smooth muscle actin, as well as collagen type I, III and IV, whilst reversing the decrease of E‑cadherin in HG‑induced MES13 cells. It was further revealed that circRNA_0000491 sponged miR‑101b and that miR‑101b directly targets TGFβRI. In addition, the expression levels of miR‑101b were negatively associated with the transcriptional level of circRNA_0000491 and miR‑101b inhibitors reversed the suppression of extracellular matrix (ECM)‑associated protein synthesis mediated by knocking‑down circRNA_0000491. In conclusion, the present study investigated the circRNA_0000491/miR‑101b/TGFβRI axis in ECM accumulation and fibrosis‑associated protein expression levels of mesangial cells, which suggested that circRNA_0000491 may be beneficial for the development of an effective therapeutic target for DN.
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http://dx.doi.org/10.3892/mmr.2020.11486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533486PMC
November 2020

An early experience of the robot-assisted laparoscopic nephroureterectomy without re-docking in a single position.

Ann Ital Chir 2020 ;91:327-333

Objective: This study aims to evaluate the execution of robot-assisted laparoscopic nephroureterectomy without repositioning the patient.

Methods: The clinical data of 9 patients who underwent robot-assisted laparoscopic nephroureterectomy between May 2017 and November 2018 were analyzed, proceeding in a single position, without repositioning the patient. This involved 5 men and 4 women, with an average age of 61.67 ± 10.37 years and an average body mass index (BMI) of 24.78 ± 3.84. We considered the duration of the intervention, the blood loss, the duration of the hospital stay, the duration of maintenance of the drainage and the follow-up on all patients, with or without complications and recurrence of the tumor.

Results: The intervention was completed in all 9 cases. The average duration of the intervention was 242.89 ± 13.37 minutes, the average blood loss was 166.67 ± 70.71 ml, the average hospitalization time was 2 ± 0.71 days, the average time drainage maintenance was 5.11 ± 1.05 days and the average follow-up times without complications and tumor recurrence were 12.56 ± 6.19 months.

Conclusion: Robot-assisted laparoscopic nephroureterectomy without repositioning the patient during the procedure simplifies the procedure and shortens the duration of the procedure. It is also a safe, effective and feasible minimally invasive treatment method.

Key Words: Nephroureterectomy, Robot-assisted laparoscopic, Tumor recurrence, Single position, Upper tract urothelial carcinoma.
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January 2020

Expression regulation of MALATE SYNTHASE involved in glyoxylate cycle during protocorm development in Phalaenopsis aphrodite (Orchidaceae).

Sci Rep 2020 06 22;10(1):10123. Epub 2020 Jun 22.

Institute of Tropical Plant Sciences and Microbiology, National Cheng Kung University, Tainan, Taiwan.

Orchid (Orchidaceae) is one of the largest families in angiosperms and presents exceptional diversity in lifestyle. Their unique reproductive characteristics of orchid are attracted by scientist for centuries. One of the synapomorphies of orchid plants is that their seeds do not contain endosperm. Lipids are used as major energy storage in orchid seeds. However, regulation and mobilization of lipid usage during early seedling (protocorm) stage of orchid is not understood. In this study, we compared transcriptomes from developing Phalaenopsis aphrodite protocorms grown on 1/2-strength MS medium with sucrose. The expression of P. aphrodite MALATE SYNTHASE (PaMLS), involved in the glyoxylate cycle, was significantly decreased from 4 days after incubation (DAI) to 7 DAI. On real-time RT-PCR, both P. aphrodite ISOCITRATE LYASE (PaICL) and PaMLS were down-regulated during protocorm development and suppressed by sucrose treatment. In addition, several genes encoding transcription factors regulating PaMLS expression were identified. A gene encoding homeobox transcription factor (named PaHB5) was involved in positive regulation of PaMLS. This study showed that sucrose regulates the glyoxylate cycle during orchid protocorm development in asymbiotic germination and provides new insights into the transcription factors involved in the regulation of malate synthase expression.
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http://dx.doi.org/10.1038/s41598-020-66932-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7308390PMC
June 2020

Migration regularity of phthalates in polyethylene wrap film of food packaging.

J Food Sci 2020 Jul 7;85(7):2105-2113. Epub 2020 Jun 7.

Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin, 300071, China.

As a kind of polymer material additive, phthalic acid esters (PAEs) are widely used in food industry. However, PAEs are environmental endocrine disruptors with reproductive toxicity and teratogenic carcinogenicity, which are difficult to be degraded in the natural environment. In this paper, gas chromatography-mass spectrometer (GC-MS) methods for PAEs in polyethylene wrap film were optimized. For diisobutyl phthalate (DIBP) and dibutyl phthalate (DBP) that were mainly detected, the method had a good linearity in 1 to 500 ng/g. Then, we confirmed that the migration of DIBP and DBP from polyethylene wrap film increased with time and temperature. It is found that the migration law in different food simulations well followed the migration dynamics first-level model. The rate constant K and initial release rate V are inversely proportional to the polarity of the simulated liquid. We hope that this study can serve as a valuable reference for further research on the migration of food packing materials. PRACTICAL APPLICATION: In this paper, we present a simple example of applying migration model to evaluate the migration behaviors of PAEs in food packaging materials along with their hazardous properties. It can serve as a valuable reference for further research on the migration of food packing materials.
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http://dx.doi.org/10.1111/1750-3841.15181DOI Listing
July 2020

Umpolung Strategy for the Synthesis of Chiral Dispiro[2-amino-4,5-dihydrofuran-3-carbonitrile]bisoxindoles.

J Org Chem 2020 06 10;85(12):7793-7802. Epub 2020 Jun 10.

Zhejiang Engineering Laboratory for Green Syntheses and Applications of Fluorine-Containing Specialty Chemicals, Institute of Advanced Fluorine-Containing Materials, Zhejiang Normal University, Jinhua 321004, P. R. China.

Based on a novel umpolung strategy, an efficient and highly enantioselective cascade aldol/cyclization/tautomerization of the 2-(2-oxoindolin-3-yl)malononitrile to active carbonyl compounds with excellent diastereo- and enantioselectivity has been developed. Also, various enantio-enriched multifunctional dispiro[2-amino-4,5-dihydrofuran-3-carbonitrile]bisoxindoles with adjacent spiro-stereocenters were conveniently obtained by this novel methodology. Also, the dispiro[2-amino-4,5-dihydrofuran-3-carbonitrile]bisoxindoles were easily transformed into structurally complex molecules without any effect on the diastereo- and enantioselectivity.
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http://dx.doi.org/10.1021/acs.joc.0c00418DOI Listing
June 2020

Recent Progress on Luminescent Metal-Organic Framework-Involved Hybrid Materials for Rapid Determination of Contaminants in Environment and Food.

Polymers (Basel) 2020 Mar 20;12(3). Epub 2020 Mar 20.

State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China.

The high speed of contaminants growth needs the burgeoning of new analytical techniques to keep up with the continuous demand for monitoring and legislation on food safety and environmental pollution control. Metal-organic frameworks (MOFs) are a kind of advanced crystal porous materials with controllable apertures, which are self-assembled by organic ligands and inorganic metal nodes. They have the merits of large specific surface areas, high porosity and the diversity of structures and functions. Latterly, the utilization of metal-organic frameworks has attracted much attention in environmental protection and the food industry. MOFs have exhibited great value as sensing materials for many targets. Among many sensing methods, fluorometric sensing is one of the widely studied methods in the detection of harmful substances in food and environmental samples. Fluorometric detection based on MOFs and its functional materials is currently one of the most key research subjects in the food and environmental fields. It has gradually become a hot research direction to construct the highly sensitive rapid sensors to detect harmful substances in the food matrix based on metal-organic frameworks. In this paper, we introduced the synthesis and detection application characteristics (absorption, fluorescence, etc.) of metal-organic frameworks. We summarized their applications in the MOFs-based fluorometric detection of harmful substances in food and water over the past few years. The harmful substances mainly include heavy metals, organic pollutants and other small molecules, etc. On this basis, the future development and possible application of the MOFs have prospected in this review paper.
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http://dx.doi.org/10.3390/polym12030691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183274PMC
March 2020

A bioinformatics and network pharmacology approach to the mechanisms of action of Shenxiao decoction for the treatment of diabetic nephropathy.

Phytomedicine 2020 Apr 22;69:153192. Epub 2020 Feb 22.

Zhejiang Chinese Medical University, 548 Binwen Road, Binjiang, Hangzhou, Zhejiang 310053, PR China. Electronic address:

Background: The epithelial-to-mesenchymal transition (EMT) of renal tubular epithelial cells is the main pathological alteration in diabetic nephropathy (DN). Traditional Chinese medicine (TCM) has been used for the treatment of DN in clinical practice and has been proven to be effective.

Purpose: This aim of this study was to shed light on the efficacy of Shenxiao decoction (SXD) on the EMT of renal tubular epithelial cells and the molecular mechanisms of SXD in mice with DN, as well as on the high glucose (HG)- and TGF-β1-induced EMT of NRK-52E and HK-2 cells.

Study Design And Methods: A bioinformatics and network pharmacology method were utilized to construct the active ingredient-target networks of SXD that were responsible for the beneficial effects against DN. The effects of RUNX3 were validated in HG- and TGF-β1-induced EMT processes in NRK-52E and HK-2 cells.

Results: Bioinformatics analysis revealed that 122 matching targets were closely associated with the regulation of cell migration and the AGE-RAGE signaling pathway in diabetic complications. The results also revealed that, relative to the mice with DN, the mice in the treatment group had an improved general state and reduced blood glucose levels. The degradation of renal function was ameliorated by SXD. Moreover, the protective effects of SXD were also observed on renal structural changes. Furthermore, SXD suppressed the activation of the transforming growth factor (TGF)-β1/Smad pathway and upregulated the RUNX3 and E-cadherin levels and downregulated the extracellular matrix (ECM) protein levels in mice with DN. SXD was further found to prevent the HG- and TGF-β1-induced EMT processes in NRK-52E and HK-2 cells. Additionally, the overexpression of RUNX3 markedly inhibited the EMT and TGF-β1/Smad pathway induced by HG and TGF-β1 in NRK-52E and HK-2 cells.

Conclusion: Taken together, these results suggest that SXD maybe alleviate EMT in DN via the inhibition of the TGF-β1/Smad/RUNX3 signaling pathway under hyperglycemic conditions.
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http://dx.doi.org/10.1016/j.phymed.2020.153192DOI Listing
April 2020

A Mild Synthesis of 2-Substituted Benzothiazoles via Nickel-Catalyzed Intramolecular Oxidative C-H Functionalization.

J Org Chem 2020 Jan 31;85(2):493-500. Epub 2019 Dec 31.

Hubei Key Laboratory of Radiation Chemistry and Functional Materials, School of Nuclear Technology and Chemistry & Biology , Hubei University of Science and Technology , Xianning 437100 , China.

A highly efficient synthetic method for the preparation of 2-aminobenzothiazoles starting from arylthioureas has been reported. By using a nickel catalyst, arylthioureas undergo intramolecular oxidative C-H bond functionalization, giving the desired 2-aminobenzothiazoles in good to excellent yields. This protocol features an inexpensive catalyst, low catalyst loading, mild reaction conditions, a short reaction time, and good to excellent yields, and it can be scaled up easily to a gram scale with almost no yields decreasing.
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http://dx.doi.org/10.1021/acs.joc.9b02543DOI Listing
January 2020

Efficient travelling-mode quantum key agreement against participant's attacks.

Sci Rep 2019 Nov 11;9(1):16421. Epub 2019 Nov 11.

State Key Laboratory for Novel Software Technology, Nanjing University, Nanjing, 210046, P. R. China.

Quantum key agreement (QKA) is to negotiate a final key among several participants fairly and securely. In this paper, we show that some existing travelling-mode multiparty QKA protocols are vulnerable to internal participant's attacks. Dishonest participants can exploit a favorable geographical location or collude with other participants to predetermine the final keys without being discovered. To resist such attacks, we propose a new travelling-mode multiparty QKA protocol based on non-orthogonal Bell states. Theoretical analysis shows that the proposed protocol is secure against both external and internal attacks, and can achieve higher efficiency compared with existing travelling-mode multiparty QKA protocols. Finally we design an optical platform for each participant, and show that our proposed protocol is feasible with current technologies.
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http://dx.doi.org/10.1038/s41598-019-51987-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848133PMC
November 2019

Development of a Library of Thiophene-Based Drug-Like Lego Molecules: Evaluation of Their Anion Binding, Transport Properties, and Cytotoxicity.

Chemistry 2020 Jan 27;26(4):888-899. Epub 2019 Dec 27.

Department of Chemistry, CICECO - Aveiro Institute of Materials, University of Aveiro, 3810-193, Aveiro, Portugal.

The anion-binding and transport properties of an extensive library of thiophene-based molecules are reported. Seventeen bis-urea positional isomers, with different binding conformations and lipophilicities, have been synthesized by appending α- or β-thiophene or α-, β-, or γ-benzo[b]thiophene moieties to an ortho-phenylenediamine central core, yielding six subsets of positional isomers. Through H NMR, X-ray crystallography, molecular modelling, and anion efflux studies, it is demonstrated that the most active transporters adopt a pre-organized binding conformation capable of promoting the recognition of chloride, using urea and C-H binding groups in a cooperative fashion. Additional large unilamellar vesicle-based assays, carried out under electroneutral and electrogenic conditions, together with N-methyl-d-glucamine chloride assays, have indicated that anion efflux occurs mainly through an H /Cl symport mechanism. On the other hand, the most efficient anion transporter displays cytotoxicity against tumor cell lines, while having no effects on a cystic fibrosis cell line.
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http://dx.doi.org/10.1002/chem.201904255DOI Listing
January 2020

The complete chloroplast genome of (Orchidaceae).

Mitochondrial DNA B Resour 2019 Oct 25;4(2):3776-3777. Epub 2019 Oct 25.

Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, Shenzhen, China.

Lindl is an epiphytic orchid with significant ornamental values. Here, we report the first complete chloroplast genome of . The complete chloroplast (cp) genome sequence of is 160,024 bp in length and consisted of two inverted repeats (IRs, 25,403 bp), which were separated by a large single copy region (LSC, 88,075 bp) and a small single copy region (SSC, 21,143 bp). The cp genome encoded 142 genes, of which 110 were unique genes (80 protein-coding genes, 26 tRNAs and 4 rRNAs). Phylogenetic analysis showed that clustered together with .
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http://dx.doi.org/10.1080/23802359.2019.1666049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7706476PMC
October 2019

The complete chloroplast genome of (Orchidaceae).

Mitochondrial DNA B Resour 2019 Sep 23;4(2):3200-3201. Epub 2019 Sep 23.

Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, Shenzhen, Guangdong, China.

is an endangered species of Orchidaceae. Here we report the complete chloroplast (cp) genome sequence and the cp genome features of . The complete cp genome sequence of is 157,292 bp in length and presented a typical quadripartite structure including one large single-copy region (LSC, 86,583 bp), one small single-copy region (SSC, 19,449 bp), and two inverted repeat regions (IRs, 25,630 bp each). The cp genome encoded 138 genes, of which 120 were unique genes. The phylogenetic relationships show that is closely related to other species in .
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http://dx.doi.org/10.1080/23802359.2019.1669088DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707279PMC
September 2019

Photoswitchable Förster resonance energy transfer (FRET) within a heterometallic Ir-Pt macrocycle.

Chem Commun (Camb) 2019 Sep 28;55(74):11119-11122. Epub 2019 Aug 28.

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, P. R. China.

A new heterometallic macrocycle with photochromic properties was succesfully constructed through coordination-driven self-assembly, which features interesting photoswitchable Förster resonance energy transfer (FRET) behaviour.
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http://dx.doi.org/10.1039/c9cc05377hDOI Listing
September 2019
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