Publications by authors named "Li Zeng"

737 Publications

Selective Interface Synthesis of Cobalt Metaphosphate Nanosheet Arrays Motivated by Functionalized Carbon Cloths for Fast and Durable Na/K-Ion Storage.

ACS Appl Mater Interfaces 2021 Jul 19. Epub 2021 Jul 19.

State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute, Sichuan University, Chengdu 610065, China.

Exploiting novel nanomaterials with fast and durable sodium/potassium ion storage capability is key to alleviate the application limitations of lithium-ion batteries. Herein, a novel energy storage material based on cobalt metaphosphate nanosheet arrays self-supported on carbon cloths [Co(PO) NSs/CC] is fabricated by a two-step strategy. This rationally designed strategy avoids the preparation of the complex {Co[OP(OBu)]} precursor, which significantly simplifies the synthesis process. The active CC acts not only as an electrically conductive substrate as usual but also as a functional basis to suppress PH-involved reaction and to promote HPO-involved reaction during the phosphating process, contributing to the formation of Co(PO). The mutual cross-linked porous Co(PO) nanosheets vertically grow on the surface of activated CC, ensuring sufficient electrolyte infiltration and fast electron transport among the electrodes. Sodium ion storage analysis for the Co(PO) NSs/CC electrode reveals a multi-step reaction mechanism with high reversibility, as reflected by the high reversible capacity (667 mA h g at 50 mA g) and excellent cyclability (with almost no capacity decay over 500 cycles). This novel electrode is also well capable of storing potassium ions, exhibiting high reversible capacity, which outperforms most reported anodes for potassium-ion batteries. The development of this novel high-performance nanomaterial would advance the performance of sodium/potassium-ion batteries toward practical applications.
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http://dx.doi.org/10.1021/acsami.1c08950DOI Listing
July 2021

Temperature-compensated optical fiber sensor for volatile organic compound gas detection based on cholesteric liquid crystal.

Opt Lett 2021 Jul;46(14):3324-3327

External temperature variations inevitably affect the accuracy of a liquid crystal sensor. Therefore, we propose a novel temperature-compensated fiber volatile organic compound (VOC, using acetone as a model compound) gas sensor. The proposed sensor consists of a short segment of hollow-core fiber (HCF), which is spliced on a multimode fiber. Cholesteric liquid crystal (CLC) is sealed into HCF to sense the temperature, and another type of CLC is coated on the end face of HCF for VOC gas detection. The VOC gas concentration and ambient temperature can be simultaneously measured by monitoring the wavelength shifts of two Bragg reflection peaks caused by two types of CLCs. The effects of the CLC thickness on the sensitivities of temperature and acetone concentration are investigated, and optimal parameters are chosen. An optimal sensor can reach a temperature sensitivity of 2.53 nm/°C and acetone concentration sensitivity of 48.46 nm·L/mmol at 8-44°C. In addition, temperature compensation capability, repeatability, response time, and stability are also researched. The experimental results prove this sensor has great application potential in high-precision real-time VOC gas monitoring and detection.
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http://dx.doi.org/10.1364/OL.427606DOI Listing
July 2021

Sleep disturbance mediates the relationship between depressive symptoms and cognitive function in older adults with mild cognitive impairment.

Geriatr Nurs 2021 Jul 10;42(5):1019-1023. Epub 2021 Jul 10.

Shenzhen Qianhai Shekou Free Trade Zone Hospital, No.36, 7th Industrial Road, Nanshan District, Shenzhen City, Guangdong province, 518067, China. Electronic address:

This study examined whether and to what extent sleep disturbance mediates the effects of depressive symptoms on the cognition of individuals with mild cognitive impairment (MCI), who represent a high-risk group for developing dementia. Cross-sectional data were obtained from a sample of 204 Chinese community-dwelling older adults with MCI. MCI subjects were screened using the Montreal Cognitive Assessment, sleep quality was measured using the Pittsburgh Sleep Quality Index, and depressive symptoms were assessed using the Geriatric Depression Scale. Mediation analysis was conducted using the PROCESS macro with 10,000 bootstrap samples. The significant mediating effect of sleep quality on the association between depressive symptoms and cognition (Beta = -0.025; 95% CI, -0.054 to -0.007) explains 26% of the total effect of depressive symptoms on cognition and implies that the timely detection and management of sleep disturbance among the MCI population is highly important, especially for those with depressive symptoms.
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http://dx.doi.org/10.1016/j.gerinurse.2021.06.004DOI Listing
July 2021

Evaluation of Peroxidase in Herbal Medicines Based on an Electrochemical Sensor.

Front Chem 2021 23;9:709487. Epub 2021 Jun 23.

Department of Anorectal, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, China.

Peroxidases are species-specific. Differences in peroxidase can objectively reflect the genetics among species. The use of peroxidase to assist in species identification is relatively simple and effective. In this work, we proposed a graphene-modified electrode. This electrode can amplify the signal of electrocatalytic reduction of hydrogen peroxide. Since peroxidase can catalyze the reduction of hydrogen peroxide, this signal can be used as an indicator to demonstrate the content of peroxidase in different plant tissues. Twelve herbal medicines were selected for our study. The results show that this electrochemical-based detection technique was comparable to colorimetric method in terms of accuracy.
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http://dx.doi.org/10.3389/fchem.2021.709487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260690PMC
June 2021

Complete mitochondrial genome of banana skipper Evans (Lepidoptera: Hesperiidae) and phylogenetic analysis.

Mitochondrial DNA B Resour 2021 Jun 23;6(7):2054-2055. Epub 2021 Jun 23.

Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province, Agricultural Environment and Resource Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China.

(Evans, 1941) is a banana pest and is mainly distributed in Southeast Asia and the Pacific regions. The complete mitogenome of (GenBank accession number MW586888) is 15,987 bp in size, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes, and a noncoding A + T-rich region. The A + T-rich region is located between and . The base composition of the whole mitogenome is 39.68% for A, 7.30% for G, 41.55% for T, and 11.47% for C, with a high AT content of 81.23%. The phylogeny analysis indicated that had a close relationship with . The present data could contribute to the further detailed phylogeographic analysis and provide a comprehensive control strategy for this banana pest.
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http://dx.doi.org/10.1080/23802359.2021.1942264DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231347PMC
June 2021

The association between family impact and health-related quality of life of children with idiopathic central precocious puberty in Chongqing, China.

Health Qual Life Outcomes 2021 Jun 26;19(1):171. Epub 2021 Jun 26.

Department of General Medicine, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University, Jinyu Avenue No. 20, Yubei, Chongqing, 400010, China.

Background: Idiopathic central precocious puberty (ICPP) reduces patient health-related quality of life (HRQoL). The impacts of disease and treatment on families are also an important concern. This study aimed to assess the association between family impact and HRQoL of children with ICPP.

Methods: We conducted a case-control study in Chongqing, China. A case group of 134 children with ICPP aged 5-12 years and their caregivers was recruited from a children's hospital in Chongqing. A total of 210 gender- and age-matched subjects from two primary schools were selected as controls. PedsQL4.0 Generic Core Scales (GCS) and PedsQL™ Family Impact Module (FIM) were used in this study.

Results: Children with ICPP scored lower than controls in all HRQoL domains except physical functioning. In particular, the two groups were significantly different in emotional functioning scores (d = 0.414, P < 0.001). Compared with controls, ICPP families had lower scores in all dimensions of the FIM scale (d = 0.288-1.030, all P < 0.05). Factors associated with HRQoL of ICPP patients included: age of patients, type of medical treatment, employment status of caregivers, educational level of caregivers, parent HRQoL and family functioning (all P < 0.05).

Conclusions: Children with ICPP demonstrated lower quality of life and greater family impact compared to healthy controls. In addition, less impact of disease on parent HRQoL and family functioning was associated with better HRQoL of ICPP patients, patients aged older, treated with drug combination, cared by employed or well-educated caregivers reported better HRQoL. Health care professionals should pay more attention to younger patients treated with GnRHa alone, and provide targeted interventions for caregivers depending on their demographic background to reduce family impact and thereby improve patient HRQoL.
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http://dx.doi.org/10.1186/s12955-021-01805-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236152PMC
June 2021

Rational design of Pt-Pd-Ni trimetallic nanocatalysts for room-temperature benzaldehyde and styrene hydrogenation.

Chem Asian J 2021 Jun 22. Epub 2021 Jun 22.

Department of Chemistry, The University of Hong Kong, Hong Kong SAR, P. R. China.

Nanostructures of the multimetallic catalysts offer great scope for fine tuning of heterogeneous catalysis, but clear understanding of the surface chemistry and structures is important to enhance their selectivity and efficiency. Focussing on a typical Pt-Pd-Ni trimetallic system, we comparatively examined the Ni/C, Pt/Ni/C, Pd/Ni/C and Pt-Pd/Ni/C catalysts synthesized by impregnation and galvanic replacement reaction. To clarify surface chemical/structural effect, the Pt-Pd/Ni/C catalyst was thermally treated at X=200, 400 or 600 °C in a H reducing atmosphere, respectively termed as Pt-Pd/Ni/C-X. The as-prepared catalysts were characterized complementarily by XRD, XPS, TEM, HRTEM, HS-LEIS and STEM-EDS elemental mapping and line-scanning. All the catalysts were comparatively evaluated for benzaldehyde and styrene hydrogenation. It is shown that the "PtPd alloy nanoclusters on Ni nanoparticles" (PtPd/Ni) and the synergistic effect of the trimetallic Pt-Pd-Ni, lead to much improved catalytic performance, compared with the mono- or bi- metallic counterparts. However, with the increase of the treatment temperature of the Pt-Pd/Ni/C, the catalytic performance was gradually degraded, which was likely due to that the favourable nanostructure of fine "PtPd/Ni" was gradually transformed to relatively large "PtPdNi alloy on Ni" (PtPdNi/Ni) particles, thus decreasing the number of noble metal (Pt and Pd) active sites on the surface of the catalyst. The optimum trimetallic structure is thus the as synthesised Pt-Pd/Ni/C. This work provides a novel strategy for the design and development of highly efficient and low-cost multimetallic catalysts, e. g. for hydrogenation reactions.
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http://dx.doi.org/10.1002/asia.202100472DOI Listing
June 2021

Relationship between levels of serum gastric inhibitory polypeptide (GIP), soluble interleukin-2 receptor (sIL-2R), and soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) and disease condition and prognosis of patients with severe acute pancreatitis.

Ann Palliat Med 2021 Jun 11;10(6):6786-6792. Epub 2021 Jun 11.

Chinese Academy of Sciences, Sichuan Translational Medicine Research Hospital, Chengdu, China; RICU & MICU, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Background: To explore the relationship between levels of serum gastric inhibitory polypeptide (GIP), soluble interleukin-2 receptor (sIL-2R), and soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) and the disease condition and prognosis in patients with severe acute pancreatitis (SAP).

Methods: A total of 52 patients with SAP (SAP group) and 50 patients with mild acute pancreatitis (MAP group) admitted to Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China between April 2017 and December 2019 were included in the present study. A further 50 people who had received a healthy physical examination during the same period constituted the healthy control group. The levels of serum GIP, sIL-2R, and sTREM-1 were measured. The levels of serum GIP, sIL-2R, and sTREM-1 were compared among the SAP, MAP and healthy control groups, and the severity of disease (Ranson scoring system) was compared between the SAP and MAP groups. Pearson correlation analysis was used to analyze the correlation between the levels of serum GIP, sIL-2R, and sTREM-1 with the Ranson scores in the SAP group. A receiver operating characteristic (ROC) curve was drawn to evaluate the predictive efficacy of serum GIP, sIL-2R, and sTREM-1 on prognosis.

Results: The levels of serum GIP, sIL-2R, and sTREM-1 in the SAP and MAP groups were higher than those in the healthy control group, and the levels in the SAP group were higher than those in the MAP group. The Pearson correlation analysis showed that the levels of serum GIP, sIL-2R, and sTREM-1 in the SAP group were positively correlated with Ranson scores. The levels of serum GIP, sIL-2R, and sTREM-1 in the survival group were lower than those in the deceased group. The ROC curve showed that the best cut-off values of serum GIP, sIL-2R, and sTREM-1 in predicting prognostic survival were 167.040 pg/mL, 70.840 pg/mL, and 128.325 ng/mL, respectively.

Conclusions: The levels of serum GIP, sIL-2R, and sTREM-1 are closely related to the severity of illness in patients with SAP and can be used as reference indicators for assessing the onset of SAP and predicting prognosis.
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http://dx.doi.org/10.21037/apm-21-1231DOI Listing
June 2021

Management and outcomes of surgical patients with intestinal Behçet's disease and Crohn's disease in southwest China.

World J Clin Cases 2021 Jun;9(16):3858-3868

Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.

Background: Gastrointestinal involvement in Behçet's disease (GIBD) and Crohn's disease (CD) are inflammatory diseases sharing a considerable number of similarities. However, different from CD, the operative and postoperative management of GIBD remains largely empirical because of the lack of comprehensive treatment guidelines.

Aim: To compare surgical patients with GIBD and those with CD in a medical center and identify notable clinical features and effective postoperative treatment for surgical patients with GIBD.

Methods: We searched patients diagnosed with CD and GIBD who underwent operations for gastrointestinal complications from 2009 to 2015 at West China Hospital of Sichuan University. A total of 10 surgical patients with GIBD and 106 surgical patients with CD were recruited. Information including demographic data, medication, and operative and postoperative parameters were collected and analyzed. As the incidence of surgical GIBD is low, their detailed medical records were reviewed and compared to previous studies. Moreover, the prognoses of CD and GIBD were evaluated respectively between groups treated with biological and non-biological agents.

Results: Indication for first surgery was often acute intestinal perforation for GIBD patients (7/10 0/106, 0.001), whereas intestinal fistulae (0/10 44/106, = 0.013) and ileus (0/10 40/106, = 0.015) were the indications for surgical CD patients. Approximately 40% of patients with GIBD and 23.6% of patients with CD developed postoperative complications, 50% of patients with GIBD and 38.7% of patients with CD had recurrence postoperatively, and 40% (4/10) of patients with GIBD and 26.4% (28/106) of patients with CD underwent reoperations. The average period of postoperative recurrence was 7.87 mo in patients with Behçet's disease (BD) and 10.43 mo in patients with CD, whereas the mean duration from first surgery to reoperation was 5.75 mo in BD patients and 18.04 mo in CD patients. Surgical patients with GIBD more often used corticosteroids (6/10 7/106, 0.001) and thalidomide (7/10 9/106, 0.001) postoperatively, whereas surgical patients with CD often used infliximab (27/106), azathioprine, or 6-mercaptopurine (74/106) for maintenance therapy.

Conclusion: Patients suffering GIBD require surgery mostly under emergency situations, which may be more susceptible to recurrence and reoperation and need more aggressive postoperative treatment than patients with CD.
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http://dx.doi.org/10.12998/wjcc.v9.i16.3858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180223PMC
June 2021

Identifying the Persistent Free Radicals (PFRs) Formed as Crucial Metastable Intermediates during Peroxymonosulfate (PMS) Activation by N-Doped Carbonaceous Materials.

Environ Sci Technol 2021 07 17;55(13):9293-9304. Epub 2021 Jun 17.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, P. R. China.

A nonradical mechanism involved in peroxymonosulfate (PMS) activation in carbonaceous materials (CMs) is still controversial. In this study, we prepared N-doped CMs, including hollow carbon spheres (NHCSs) and carbon nanotubes (N-CNTs), to probe the crucial intermediates during PMS activation. The results suggested that the higher efficiency and lower activation energy (13.72 kJ mol) toward phenol (PN) degradation in an NHCS/PMS system than PMS alone (∼24.07 kJ mol) depended on a typical nonradical reaction. Persistent free radicals (PFRs) with a factor of 2.0033-2.0045, formed as crucial metastable intermediates on NHCS or N-CNT in the presence of PMS, contribute largely to the organic degradation (∼73.4%). Solid evidence suggested that the formation of PFRs relied on the attack of surface-bonded OH and SO or peroxides in PMS, among which surface-bonded SO was most thermodynamically favorable based on theoretical calculations. Electron holes within PFRs on NHCSs shifted the Fermi level to the positive energy with the valance band increasing from 1.18 to 1.98 eV, promoting the reactivity toward nucleophilic substances. The degradation intermediates of aromatic compounds (e.g., PN) and electron rearrangement triggered the evolution of PFRs from oxygen-centered to carbon-centered radicals. Moreover, due to the specific electron configuration, graphitic N on NHCS was critical for stabilizing the PFRs. This study provides insightful understanding of the fate of organic contaminants and the structure-activity relationship of reactivity of CMs toward PMS activation.
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http://dx.doi.org/10.1021/acs.est.1c01974DOI Listing
July 2021

Long Noncoding RNA GAS5 Targeting miR-221-3p/Cyclin-Dependent Kinase Inhibitor 2B Axis Regulates Follicular Thyroid Carcinoma Cell Cycle and Proliferation.

Pathobiology 2021 15;88(4):289-300. Epub 2021 Jun 15.

Department of Oncology, Xiangya Hospital, Central South University, Changsha, China.

Introduction: Follicular thyroid carcinoma (FTC) is more aggressive than the most common papillary thyroid carcinoma (PTC). However, the current research on FTC is less than PTC. Here, we investigated the effects of long noncoding RNA (lncRNA) GAS5 and miR-221-3p in FTC.

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to detect GAS5 and miR-221-3p expression in the FTC tissues and cells. Cell proliferation was assessed by CCK8 and EdU assays. Flow cytometry was performed to determine the cell cycle. The dual-luciferase reporter assay was employed to validate the binding relationship of GAS5/miR-221-3p and miR-221-3p/cyclin-dependent kinase inhibitor 2B (CDKN2B). Western blot was conducted to measure the protein level of CDKN2B.

Results: Our results displayed that GAS5 was downregulated, while miR-221-3p was upregulated in FTC tissues and cells. What's more, overexpression of GAS5 or miR-221-3p inhibition induced G0/G1 phase arrest and inhibited cell proliferation of FTC cells. GAS5 acted as a sponge of miR-221-3p, and CDKN2B was a target gene of miR-221-3p. Additionally, GAS5 inhibited cell cycle and proliferation of FTC cells via reducing miR-221-3p expression to enhance CDKN2B expression.

Conclusion: GAS5 induced G0/G1 phase arrest and inhibited cell proliferation via targeting miR-221-3p/CDKN2B axis in FTC. Thus, GAS5 may be a potential therapeutic target for the treatment of FTC.
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http://dx.doi.org/10.1159/000513338DOI Listing
June 2021

Finding key players in complex networks through deep reinforcement learning.

Nat Mach Intell 2020 Jun 25;2(6):317-324. Epub 2020 May 25.

Channing Division of Network Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, 02115, USA.

Finding an optimal set of nodes, called key players, whose activation (or removal) would maximally enhance (or degrade) certain network functionality, is a fundamental class of problems in network science. Potential applications include network immunization, epidemic control, drug design, and viral marketing. Due to their general NP-hard nature, those problems typically cannot be solved by exact algorithms with polynomial time complexity. Many approximate and heuristic strategies have been proposed to deal with specific application scenarios. Yet, we still lack a unified framework to efficiently solve this class of problems. Here we introduce a deep reinforcement learning framework FINDER, which can be trained purely on small synthetic networks generated by toy models and then applied to a wide spectrum of influencer finding problems. Extensive experiments under various problem settings demonstrate that FINDER significantly outperforms existing methods in terms of solution quality. Moreover, it is several orders of magnitude faster than existing methods for large networks. The presented framework opens up a new direction of using deep learning techniques to understand the organizing principle of complex networks, which enables us to design more robust networks against both attacks and failures.
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http://dx.doi.org/10.1038/s42256-020-0177-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191335PMC
June 2020

Synthesis and evaluation of the anti-inflammatory activity of novel 8-quinolinesulfonamide derivatives as TLR4/MD-2 inhibitors with efficacy in adjuvant-induced arthritis.

Bioorg Chem 2021 May 31;114:105037. Epub 2021 May 31.

Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University, Hefei 230032, China. Electronic address:

In this study, a series of 8-quinolinesulfonamidederivatives was synthesized, and their anti-inflammatory activity was evaluated. Among them, compound 3l was found to be the best anti-inflammatory agent, with IC values of 2.61 ± 0.39, 9.74 ± 0.85, and 12.71 ± 1.34 μM against NO, TNF-α and IL-1β production respectively. And 3l could significantly prevent lipopolysaccharide (LPS)-induced expression of inflammatory mediators (iNOS and COX-2). Molecule docking results showed that 3l could bind to the LPS binding site of toll-like receptor 4 (TLR4)/MD-2, and 3l was then identified as TLR4/MD-2 inhibitor by co-immunoprecipitation (co-IP) and cellular thermal shift assay (CTESA). Preliminary mechanism studies indicated that 3l could prevent TLR4 from being activated by disrupting TLR4/MD-2 heterodimerization and TLR4 homodimerization, thereby blocking the activation of the NF-κB/MAPK signaling pathway. Furthermore, observation of rat foot swelling, joint pathology and serum inflammatory cytokine levels proved that compound 3l had a significant therapeutic effect on adjuvant-induced arthritis (AIA) in rats in vivo. These results indicated that compound 3l is a potential anti-inflammatory agent, from which more effective anti-inflammatory drugs could be developed.
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http://dx.doi.org/10.1016/j.bioorg.2021.105037DOI Listing
May 2021

CIRBP promotes ferroptosis by interacting with ELAVL1 and activating ferritinophagy during renal ischaemia-reperfusion injury.

J Cell Mol Med 2021 Jun 10. Epub 2021 Jun 10.

Department of Organ Transplantation, Shanghai Changhai Hospital, Shanghai, China.

Renal ischaemia-reperfusion (IR) is a major cause of acute kidney injury (AKI). Cold-inducible RNA-binding protein (CIRBP) may contribute to AKI because its deficiency protects against renal IR injury in a mechanism believed to involve ferroptosis. We aimed to investigate whether ferroptosis is associated with CIRBP-mediated renal damage. The differential expression of CIRBP was examined in tubular epithelial (HK2) cells during hypoxia-reoxygenation (HR) or in response to erastin, an inducer of ferroptosis. CIRBP expression was increased in response to HR or erastin in HK2 cells but the silencing of CIRBP inhibited HR and erastin-induced ferroptosis together with ferritinophagy. We discovered an interaction between CIRBP and ELAVL1 using STRING software, which was verified through co-immunoprecipitation and fluorescence colocalization assays. We found that ELAVL1 is a critical regulator in the activation of ferritinophagy and the promotion of ferroptosis. HR or erastin also induced the expression of ELAVL1. An autophagy inhibitor (hydroxychloroquine) or si-ELAVL1 transfection reversed CIRBP-enhanced ferritinophagy activation and ferroptosis in HK2 cells under HR. Injection of anti-CIRBP antibody into a mouse model of IR inhibited ferroptosis and decreased renal IR injury in vivo. In summary, our results provide evidence that ferritinophagy-mediated ferroptosis could be responsible for CIRBP-enhanced renal IR injury.
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http://dx.doi.org/10.1111/jcmm.16567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256344PMC
June 2021

Analysis of the characteristic compounds of Citri Sarcodactylis Fructus from different geographical origins.

Phytochem Anal 2021 Jun 10. Epub 2021 Jun 10.

College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Introduction: Citri Sarcodactylis Fructus (CSF) is widely used as a food ingredient and a traditional Chinese medicine. In China, CSF is cultivated in many places, including Sichuan, Guangdong, Zhejiang, and Fujian provinces. The types and chemical contents of CSF from different origins may vary greatly due to the difference in climate and environmental conditions. Therefore, comparing the chemical composition of CSF from various places is vital.

Objective: To rapidly select potential characteristic compounds for differentiating CSF from different origins.

Material And Methods: Thirty-one batches of CSF samples from different regions were analysed using ultra-performance liquid chromatography with hybrid quadrupole-orbitrap high-resolution mass spectrometry. Thereafter, chemometric methods, including principal component analysis (PCA) and orthogonal partial least squares discrimination analysis (OPLS-DA), were employed to find differential metabolites among the CSF samples from various origins.

Results: PCA revealed 77.9% of the total variance and divided all CSF samples into three categories corresponding to their origins. OPLS-DA displayed better discrimination of CSF from different sources, with R X, R Y, and Q of 0.801, 0.985, and 0.849, respectively. Finally, 203 differential metabolites were obtained from CSF from different origins using the variable importance in projection of the OPLS-DA model, 30 of which were identified, and five coumarin compounds were selected as marker compounds discriminating CSF from different origins.

Conclusion: This work provides a practical strategy for classifying CSF from different origins and offers a research foundation for the quality control of CSF.
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http://dx.doi.org/10.1002/pca.3069DOI Listing
June 2021

Gait retraining for runners with patellofemoral pain: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 May;100(19):e25789

Department of Orthopedics, Bijie Traditional Chinese Medicine Hospital, People's Republic of China.

Introduction: Patellofemoral pain (PFP) is highly prevalent in runners. Physical therapies were proved to be effective in the treatment of PFP. Gait retraining is an important method of physical therapy, but its effectiveness and safety for PFP remained controversial. Previous review suggests gait retraining in the treatment of PFP warrants consideration. However, recent publications of randomized controlled studies and case series studies indicated the positive effect of gait retraining in clinical and functional outcomes, which re-raise the focus of gait retraining. This paper will systematically review the available evidence, assessing the safety and effectiveness for the use of gait retraining for runners with PEP.

Method And Analysis: A systematic review of relevant studies in Pubmed, Embase, SCOPUS, and Cochrane Library were synthesized. Inclusion criteria are studies evaluating clinical outcomes (i.e., changes to pain and/or function) following running retraining interventions in symptomatic running populations; Studies with less than 10 participants in total or in the running retraining intervention group were excluded. The primary outcomes measured will be pain score, Lower extremity functional scale and training related injuries or complications. Review Manager (Revman Version 5.3) software will be used for data synthesis, sensitivity analysis, meta regression, subgroup analysis and risk of bias assessment. A funnel plot will be developed to evaluate reporting bias and Begg and Egger tests will be used to assess funnel plot symmetries. We will use the Grading of Recommendations Assessment, Development and Evaluation system to assess the quality of evidence.

Ethics And Dissemination: Our aim is to publish this systematic review in a peer-reviewed journal. Our findings will provide information about the safety of gait retraining and their effect on reliving pain and improving function of lower limb on runners with PEP. This review will not require ethical approval as there are no issues about participant privacy.
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http://dx.doi.org/10.1097/MD.0000000000025789DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133046PMC
May 2021

The prevalence of compassion satisfaction and compassion fatigue among nurses: A systematic review and meta-analysis.

Int J Nurs Stud 2021 Aug 15;120:103973. Epub 2021 May 15.

College of Nursing, Chengdu University of Traditional Chinese Medicine, No.1166 Liutai Road, Wenjiang District, Chengdu City, Sichuan province, 611137, China. Electronic address:

Background: Compassion fatigue is a consequence of chronic work-related stress exposure among healthcare providers. Nursing is a high-risk, stressful profession which increases nurses' vulnerability to compassion fatigue symptoms compared to other healthcare workers. Compassion fatigue has serious consequences for nursing staff, patients and healthcare organizations. Though several studies on the prevalence of compassion fatigue among nurses have been published, the reported data vary considerably across studies; and few meta-analysis have examined the prevalence of compassion satisfaction and compassion fatigue among nurses with large sample sizes.

Objectives: To systematically assess the prevalence of compassion satisfaction and compassion fatigue among nurses, and to evaluate the effect of different geographical regions, years and departments on the prevalence of compassion fatigue.

Design: Systematic review and meta-analysis DATA SOURCES: The Cochrane Library, PubMed, EMbase, Web of Science, CINAHL, PsyclNFO, China Knowledge Resource Integrated Database (CNKI), Wanfang Database, Weipu Database (VIP), and Chinese Biomedical Database (CBM) were searched in the systematic review. The time frame for the searches included all literature before January 31st, 2020.

Review Methods: The reviewers independently completed study selection, quality assessments, data extraction and analysis of all included literature. The mean scores and standard deviations of the three subscales of the Professional Quality of Life (ProQOL) scale were pooled using random effects meta-analysis in Stata 16.0 software package. Finally, subgroup analyses were conducted to explore the sources of between-study heterogeneity.

Results: A total of 79 studies were included in the systematic review and meta-analysis, consisting of 28,509 nurses worldwide from 11 countries. In our studies, the pooled mean scores of compassion satisfaction, burnout and secondary traumatic stress were 33.12 (95% CI: 32.22-34.03), 26.64 (95% CI: 26.01-27.27) and 25.24 (95% CI: 24.69-25.79), respectively. In addition, the Asian region had the lowest levels of compassion satisfaction but the highest levels of compassion fatigue symptoms, while the Americas and Europe had the lowest levels of compassion fatigue but highest compassion satisfaction. Levels of compassionate fatigue in nurses increased gradually from 2010 to 2019, reaching the highest level in 2019; and nurses from ICU had the highest levels of compassion fatigue symptoms among all nurses.

Conclusion: The levels of compassion satisfaction and compassion fatigue among nurses are moderate. Nurses from the Asian region and in ICUs suffer from severe compassion fatigue symptoms, and the prevalence of compassion fatigue has increased over time. These findings may provide hospital administrators with the theoretical basis for the management and treatment of compassion fatigue.

Registration Number: PROSPERO [CRD42020164327].
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http://dx.doi.org/10.1016/j.ijnurstu.2021.103973DOI Listing
August 2021

Auricular acupressure for myopia prevention and control in children and its effect on choroid and retina: a randomized controlled trial protocol.

Trials 2021 Jun 7;22(1):387. Epub 2021 Jun 7.

Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.

Background: Nowadays, because of the increasing incidence, the prevention and control of myopia has become an urgent issue. In China, auricular acupressure has been commonly used in the clinical treatment of myopia in children, but the exact effectiveness remains unproven. The purpose of this trial is to observe the efficacy of auricular acupressure in myopia prevention and control, as well as its effect on the choroidal and retinal thickness.

Method/design: A total of 480 subjects at 8-9 years old will be randomized in a 1:1 ratio to an intervention group versus a control group. The intervention group will receive auricular acupressure for 12 months, while the control group will be taken as a blank control. The primary and secondary outcomes will be measured at baseline, and again at 3, 6, 9, and 12 months after recruitment. The myopia incidence (spherical equivalent ≤ - 0.50 D) and the mean change of spherical equivalent will be taken as the primary variables; the secondary outcome measures include axial length, uncorrected visual acuity, and choroidal and retinal thickness.

Discussion: This trial aims to evaluate the effectiveness of auricular acupressure for myopia prevention and control with objective evidence and to preliminarily explore the plausible mechanism and provide reference for adopting this approach to retard the onset and control the progression of myopia.

Trial Registration: Chinese Clinical Trial Registry ChiCTR2000038456 . Registered on September 23, 2020.
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http://dx.doi.org/10.1186/s13063-021-05334-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186104PMC
June 2021

[Value of chromosomal microarray analysis for the prenatal diagnosis of pregnancy with high risk signaled by non-invasive prenatal testing].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Jun;38(6):541-544

Department of Genetics and Birth Health, Changsha Maternal and Child Health Care Hospital, Changsha, Hunan 410007, China.

Objective: To explore the value of chromosomal microarray analysis (CMA) for the diagnosis of fetuses with high risk signaled by non-invasive prenatal testing (NIPT).

Methods: From June 2017 to August 2019, 628 pregnant women with high risk signaled by NIPT underwent invasive prenatal diagnosis. Amniotic fluid or cord blood samples were subjected to chromosomal karyotyping analysis or CMA. Pregnancy outcome and postnatal conditions of the fetuses were followed up.

Results: The positive predictive value for trisomy 21, trisomy 18, trisomy 13, sex chromosome aneuploidy, other rare trisomies and copy number variants (CNVs) among the 628 women were 86.4% (127/147), 41.7% (30/72), 12.9% (4/31), 43.7% (101/231), 16.5% (14/85) and 52.2% (35/67), respectively. In 218 samples with normal karyotype, 5.5% (12/218) of additional pathogenic CNVs and 2.3% (5/218) of loss of heterozygosity were detected by CMA.

Conclusion: CMA combined with karyotyping analysis can be used as first-tier test for prenatal diagnosis for women with high-risk signaled by NIPT.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200420-00285DOI Listing
June 2021

Novel Optineurin Frameshift Insertion in a Family With Frontotemporal Dementia and Parkinsonism Without Amyotrophic Lateral Sclerosis.

Front Neurol 2021 19;12:645913. Epub 2021 May 19.

Department of Neurology, National Neuroscience Institute, Singapore, Singapore.

Frontotemporal Dementia (FTD) is a common cause of Young Onset Dementia and has diverse clinical manifestations involving behavior, executive function, language and motor function, including parkinsonism. Up to 50% of FTD patients report a positive family history, supporting a strong genetic basis, particularly in cases with both FTD and amyotrophic lateral sclerosis (FTD-ALS). Mutations in three genes are associated with the majority of familial FTD (fFTD) cases - microtubule associated protein tau gene (), granulin precursor (), and hexanucleotide repeat expansions in chromosome 9 open reading frame 72- SMCR8complex subunit (C9orf72) while mutations in other genes such as optineurin () have rarely been reported. Mutations in have been reported mostly in familial and sporadic cases of ALS, or in rare cases of FTD-ALS, but not in association with pure or predominant FTD and/or parkinsonian phenotype. Here, we report for the first time, a family from the Philippines with four members harboring a novel frameshift insertion at (Chr 10:13166090 G>GA) p.Lys328GluTer11, three of whom presented with FTD-related phenotypes. Additionally, one sibling heterozygous for the frameshift insertion had a predominantly parkinsonian phenotype resembling corticobasal syndrome, but it remains to be determined if this phenotype is related to the frameshift insertion. Notably, none of the affected members showed any evidence of motor neuron disease or ALS at the time of writing, both clinically and on electrophysiological testing, expanding the phenotypic spectrum of mutations. Close follow-up of mutation carriers for the development of new clinical features and wider investigation of additional family members with further genetic analyses will be conducted to investigate the possibility of other genetic modifiers in this family which could explain phenotypic heterogeneity.
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http://dx.doi.org/10.3389/fneur.2021.645913DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170397PMC
May 2021

An Investigation into the Critical Factors Influencing the Spread of during Chicken Handling in Commercial Kitchens in China.

Microorganisms 2021 May 28;9(6). Epub 2021 May 28.

Jiangsu Key Lab of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.

Campylobacteriosis is the most common cause of bacterial gastroenteritis worldwide. Consumption of chicken meat is considered the main route for human infection with . This study aimed to determine the critical factors for cross-contamination in Chinese commercial kitchens during chicken handling. Five commercial kitchens were visited to detect occurrence from 2019 to 2020. Chicken samples (n = 363) and cotton balls from the kitchen surfaces (n = 479) were collected, and total bacterial counts and spp. were detected. Genotypic characterization of 57 isolates was performed by multilocus sequence typing (MLST). In total, 77.41% of chicken carcass samples and 37.37% of kitchen surfaces showed spp. contamination. Before chicken preparation, spp. were already present in the kitchen environment; however, chicken handling significantly increased spp. prevalence ( 0.05). After cleaning, boards, hands, and knives still showed high bacterial loads including spp., which related to poor sanitary conditions and ineffective handling practices. Poor sanitation conditions on kitchen surfaces offer greater opportunities for transmission. Molecular typing by MLST revealed that cross-contamination occurred during chicken preparation. The most prevalent sequence types, ST693 and ST45, showed strong biofilm formation ability. Consequently, sanitary condition of surfaces and biofilm formation ability of isolates were the critical points contributing to spread of in kitchen environment. These results provide insight into potential targeted control strategies along the farm-to-plate chain and highlight the necessity for improvements in sanitary conditions. The implementation of more effective cleaning measures should be considered to decrease the campylobacteriosis risk.
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http://dx.doi.org/10.3390/microorganisms9061164DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227167PMC
May 2021

Risk Score for Predicting Dysphagia in Patients After Neurosurgery: A Prospective Observational Trial.

Front Neurol 2021 11;12:605687. Epub 2021 May 11.

Department of Nursing, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, China.

Acquired dysphagia is common in patients with tracheal intubation and neurological disease, leading to increased mortality. This study aimed to ascertain the risk factors and develop a prediction model for acquired dysphagia in patients after neurosurgery. A multicenter prospective observational study was performed on 293 patients who underwent neurosurgery. A standardized swallowing assessment was performed bedside within 24 h of extubation, and logistic regression analysis with a best subset selection strategy was performed to select predictors. A nomogram model was then established and verified. The incidence of acquired dysphagia in our study was 23.2% (68/293). Among the variables, days of neurointensive care unit (NICU) stay [odds ratio (OR), 1.433; 95% confidence interval (CI), 1.141-1.882; = 0.005], tracheal intubation duration (OR, 1.021; CI, 1.001-1.062; = 0.175), use of a nasogastric feeding tube (OR, 9.131; CI, 1.364-62.289; = 0.021), and Acute Physiology and Chronic Health Evaluation (APACHE)-II C score (OR, 1.709; CI, 1.421-2.148; < 0.001) were selected as risk predictors for dysphagia and included in the nomogram model. The area under the receiver operating characteristic curve was 0.980 (CI, 0.965-0.996) in the training set and 0.971 (0.937-1) in the validation set, with Brier scores of 0.045 and 0.056, respectively. Patients who stay longer in the NICU, have a longer duration of tracheal intubation, require a nasogastric feeding tube, and have higher APACHE-II C scores after neurosurgery are likely to develop dysphagia. This developed model is a convenient and efficient tool for predicting the development of dysphagia.
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http://dx.doi.org/10.3389/fneur.2021.605687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144441PMC
May 2021

Electrochemical C-C bond cleavage of cyclopropanes towards the synthesis of 1,3-difunctionalized molecules.

Nat Commun 2021 05 24;12(1):3075. Epub 2021 May 24.

The Institute for Advanced Studies (IAS), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, People's Republic of China.

Electrochemistry has a lot of inherent advantages in organic synthesis and many redox reactions have been achieved under electrochemical condition. However, the electrochemical C-C bond cleavage and functionalization reactions are less studied. Here we develop electrochemical C-C bond cleavage and 1,3-difuntionalization of arylcyclopropanes under catalyst-free and external-oxidant-free conditions. 1,3-difluorination, 1,3-oxyfluorination and 1,3-dioxygenation of arylcyclopropanes are achieved with a high chemo- and regioselectivity by the strategic choice of nucleophiles. This protocol has good functional groups tolerance and can be scaled up. Mechanistic studies demonstrate that arylcyclopropane radical cation obtained from the anode oxidation and the subsequently generated benzyl carbonium are the key intermediates in this transformation. This development provides a scenario for constructing 1,3-difunctionalized molecules.
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http://dx.doi.org/10.1038/s41467-021-23401-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144616PMC
May 2021

The long-term observation in Chinese children with monocular myelinated retinal nerve fibers, myopia and amblyopia.

Transl Pediatr 2021 Apr;10(4):860-869

Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, China.

Background: The syndrome of monocular myelinated retinal nerve fibers (MRNF), myopia and amblyopia threatens visual development of infants and young children. The efficacy of part-time occlusion therapy remains controversial, and the long-term prognosis of the syndrome remains unclear.

Methods: Ten children (4.40±2.22 years, 4 boys and 6 girls) with monocular MRNF, myopia and amblyopia were recruited. Both the affected eyes (treatment group) and the fellow eyes (control group) underwent routine ophthalmic examinations, including AL, cycloplegic refraction, best-spectacles-corrected distance visual acuity (BSCDVA), cover test, simultaneous perception, corneal curvature, anterior segment and fundus examinations. Refractive error was corrected by either spectacles or rigid gas permeable (RGP) contact lenses. Part-time occlusion therapy was employed for amblyopia treatment. The retinal characteristics were examined only on the final visit with a Fourier-domain optical coherence tomography (FD-OCT). The speed of myopia progression and axial elongation were calculated. Statistical analysis was performed using SPSS 20 software. Cut-off P values were 0.05.

Results: On the final visit, six children had normal simultaneous perception but three had strabismus. The mean AL and spherical equivalent (SE) values of the affected eyes were 28.05±1.59 mm and -12.60±4.20 D, respectively, while those of the fellow eyes were 23.67±1.13 mm and -0.66±1.99 D, respectively. AL and SE were significantly different over time and between groups (P<0.0001), but no time*group interaction effect (P>0.05) was observed. The differences in the corneal curvature, mean speed of myopia progression, axial elongation, foveal thickness and parafoveal thickness between the two groups were not significant (P>0.05). However, the perifoveal thickness in the affected eyes was significantly higher than that of the control eyes (P=0.047). In the treatment group, the occlusion dosage positively correlated with the final BSCDVA (R=0.764, P=0.016) but did not correlate with the speed of myopia progression or axial elongation (both P values >0.05).

Conclusions: The affected eyes have longer AL, severer myopia and thicker perifoveal retina when compared with the fellow eyes. Both the affected and the fellow eyes have similar speed of myopia progression as well as that of axial elongation.
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http://dx.doi.org/10.21037/tp-20-452DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107855PMC
April 2021

Ocular Anatomical and Functional Characteristics in Anisometropic Chinese Children.

Optom Vis Sci 2021 05;98(5):476-482

Department of Computer Science and Engineering, Southern University of Science and Technology, Shenzhen, China.

Significance: This research found that anterior and posterior biometrics differ in many aspects between fellow eyes of anisometropic children. This might shed light on the mechanisms underlying the onset and progression of anisometropia and myopia.

Purpose: This study aimed to investigate the ocular biometric parameters, peripheral refraction, and accommodative lag of fellow eyes in anisometropic children.

Methods: Anisometropic children were recruited. Axial length (AL), vitreous chamber depth (VCD), central corneal thickness, anterior chamber depth (ACD), lens thickness (LT), simulated K readings, central and peripheral refractive errors, and accommodative lag were measured in both eyes. The subfoveal choroidal thickness, average choroidal thickness, and choroid vessel density of the 6 × 6-mm macular area were measured by optical coherence tomography.

Results: Thirty-two children aged 11.1 ± 1.7 years were enrolled. The average degree of anisometropia was 2.49 ± 0.88 D. The AL, VCD, ACD, and simulated K reading values were significantly larger in the more myopic eyes, whereas the LT value was significantly smaller. Subfoveal choroidal thickness (P = .001) and average choroidal thickness (P = .02) were smaller in the more myopic eyes than in the contralateral eyes, whereas choroid vessel density (P = .03) was larger. The amount of anisometropia had a significant positive correlation with the difference in AL (r = 0.869, P < .001), VCD (r = 0.853, P < .001), and ACD (r = 0.591, P < .001) and a negative correlation with the difference in LT (r = -0.457, P = .009).

Conclusions: Ocular biometrics differ in many aspects between the fellow eyes of anisometropic Chinese children, and the difference is correlated with the degree of anisometropia.
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http://dx.doi.org/10.1097/OPX.0000000000001692DOI Listing
May 2021

Administration Timing and Efficacy of Tocilizumab in Patients With COVID-19 and Elevated IL-6.

Front Mol Biosci 2021 15;8:651662. Epub 2021 Apr 15.

Department of Infection Diseases No. 1, The Maternal and Child Health Hospital of Hubei Province, Wuhan, China.

Background: Tocilizumab (TCZ), an interleukin-6 receptor antibody, has previously been used for treating patients with the coronavirus disease 2019 (COVID-19), but there is a lack of data regarding the administration timing of TCZ.

Objectives: This study aimed to evaluate the timing and efficacy of TCZ in the treatment of patients with COVID-19.

Methods: Laboratory-confirmed patients with COVID-19 with an elevated interleukin-6 (IL-6) level (>10 pg/ml) were offered TCZ intravenously for compassionate use. Clinical characteristics, laboratory tests, and chest imaging before and after the administration of TCZ were retrospectively analyzed.

Results: A total of 58 consecutive patients who met the inclusion criteria and with no compliance to the exclusion criteria were included. Of these 58 patients, 39 patients received TCZ treatment, and 19 patients who declined TCZ treatment were used as the control cohort. In the TCZ-treatment group, 6 patients (15.4%) were in mild condition, 16 (41.0%) were in severe condition, and 17 (43.6%) were in critical condition. After TCZ treatment, the condition of 27 patients (69.2%) improved and 12 (30.8%) died. Compared with the improvement group, patients in the death group had higher baseline levels of IL-6 ( = 0.0191) and procalcitonin (PCT) ( = 0.0003) and lower lymphocyte percentage (LYM) ( = 0.0059). Patients receiving TCZ treatment had better prognoses than those without TCZ treatment ( = 0.0273). Furthermore, patients with a baseline IL-6 level of ≥100 pg/ml in the TCZ-treatment group had poorer clinical outcomes than those with an IL-6 level of <100 pg/ml ( = 0.0051).

Conclusion: The administration of TCZ in an early stage of cytokine storm (IL-6 level < 100 pg/ml) may effectively improve the clinical prognosis of patients with COVID-19 by blocking the IL-6 signal pathway.
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http://dx.doi.org/10.3389/fmolb.2021.651662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084410PMC
April 2021

Preparation of Graphene Oxide-Embedded Hydrogel as a Novel Sensor Platform for Antioxidant Activity Evaluation of .

Front Chem 2021 16;9:675346. Epub 2021 Apr 16.

Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, China.

Antioxidation is very important in medicine and food. The current evaluation technologies often have many shortcomings. In this work, an improved electrochemical sensing platform for the evaluation of antioxidant activity has been proposed. A hydrogel was prepared based on graphene oxide, zinc ions, and chitosan. Zinc ions play the role of crosslinking agents in hydrogels. The structure of chitosan can be destroyed by injecting hydrogen peroxide into the hydrogel, and the free zinc ions can diffuse to the surface of the electrode to participate in the electrochemical reaction. This electrochemical sensor can evaluate the antioxidant activity by comparing the current difference of zinc reduction before and after adding the antioxidant. With the help of graphene oxide, this hydrogel can greatly enhance the sensing effect. We conducted tests on 10 real samples. This proposed electrochemical platform has been successfully applied for evaluating the antioxidant activity of , and the results were compared to those obtained from the 2,2-diphenyl-1-picrylhydrazyl-based traditional analysis technique.
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http://dx.doi.org/10.3389/fchem.2021.675346DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087177PMC
April 2021

New insights into the roles of CUL1 in mouse placenta development.

Biochem Biophys Res Commun 2021 Jun 29;559:70-77. Epub 2021 Apr 29.

Laboratory Animal Center, Chongqing Medical University, Chongqing, China. Electronic address:

CULLIN1 (CUL1) protein, as a scaffold protein in Skp1-CUL1-F box (SCF) E3 ligases complex, was reported involved in different cellular functions to regulate the early embryonic development. In our previous study, we have demonstrated that CUL1 promote trophoblast cell invasion at the maternal-fetal interface in human and the CUL1 protein significantly decreased in preeclampsia (PE) placenta, but how CUL1 involved in placentation is still obscure. Due to the embryo lethal in CUL1 knockout mice, the lentivirus mediated placenta-specific CUL1 knockdown mice model was constructed to uncover the potential role of CUL1 in placentation. In this study, CUL1 was first detected in mouse placenta. CUL1 mainly expressed in trophoblast giant cell at E9.5, and spongiotrophoblast at E11.5 and E13.5 by using immunohistochemistry and int situ hybridization. In lentivirus mediated placenta specific mouse model, the number of implanted embryos was reduced in CUL1 shRNA group at E13.5 and E18.5 compared to control group. Based on the morphological analysis of histologic staining, we observed that spongiotrophoblast layer is expanded, fetal angiogenesis in labyrinth was obstructed and fetus blood cells were accumulated in vessels. These results indicated that decreased expression of CUL1 affect placentation of mice, which give new insights into the cause of gestational diseases, but the exactly mechanism still needs further study.
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http://dx.doi.org/10.1016/j.bbrc.2021.04.064DOI Listing
June 2021

The Efficacy and Safety of Glucocorticoid on Periarticular Infiltration Analgesia in Total Knee Arthroplasty: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

J Arthroplasty 2021 Apr 26. Epub 2021 Apr 26.

Department of Orthopedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China; State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.

Background: The objective of this systematic review and meta-analysis was to examine the effect and safety of multimodal cocktail protocols implemented with or without glucocorticoids on periarticular infiltration analgesia (PIA) in patients undergoing total knee arthroplasty (TKA).

Methods: We comprehensively searched the PubMed, Medline, Embase, and Cochrane Library databases up to November 2020 for randomized controlled trials on glucocorticoids as a component of the cocktail protocol used in PIA for patients with TKA.

Results: Our meta-analysis included 11 randomized controlled trial studies with 1051 primary TKAs (930 patients). Visual analog scale scores at postoperative day (POD) 1 and POD 2 in the glucocorticoid groups were significantly lower than those in the control group (95% CI [-1.01--0.10], P = .02; 95% CI [-0.51--0.13], P = .001). Furthermore, the glucocorticoid group was associated with a statistically significant improvement in the range of motion at POD 1 (95% CI [3.20-8.05] P < .00001), reduction in total morphine consumption at 24 hours, and lower levels of C-reactive protein on POD 2/3 compared with the control group. However, neither the length of hospital stays nor the long-term Knee Society Knee Score showed any differences between the two groups. In addition, the results of subgroup analyses favored triamcinolone acetonide over betamethasone, methylprednisolone, and dexamethasone regarding the postoperative visual analog scale scores and range of motion.

Conclusion: Glucocorticoid supplementation in PIA is effective and does not increase complications or side effects for patients with either unilateral or bilateral TKA. Moreover, triamcinolone acetonide could be recommended in multimodal cocktail protocols for glucocorticoid supplementation.
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http://dx.doi.org/10.1016/j.arth.2021.03.056DOI Listing
April 2021

Discovery of 7-O-1, 2, 3-triazole hesperetin derivatives as multi-target-directed ligands against Alzheimer's disease.

Chem Biol Interact 2021 Jun 24;342:109489. Epub 2021 Apr 24.

The Key Laboratory of Major Autoimmune Diseases, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University; The Key Laboratory of Anti-inflammatory and Immune Medicines, Ministry of Education, Hefei, 230032, China. Electronic address:

The development of multi-target-directed ligands (MTDLs) may improve complex central nervous system diseases such as Alzheimer's disease (AD). Here, a series of 7-O-1, 2, 3-triazole hesperetin derivatives was evaluated for their inhibition of cholinesterase, anti-neuroinflammatory, and neuroprotective activity. Among the hesperetin derivatives, compound a8 (7-O-((1-(3-chlorobenzyl)-1H-1,2,3-triazol-4-yl)methyl)hesperetin) possessed excellent anti-butyrylcholinesterase activity (IC = 3.08 ± 0.29 μM) and exhibited good anti-neuroinflammatory activity (IC = 2.91 ± 0.47 μM) against NO production through remarkably blocking the NF-κB signaling pathway and inhibiting the phosphorylation of P65. In addition, a8 showed a remarkable neuroprotective effect and lacked neurotoxicity up to 50 μM concentration. Furthermore, possessing significant self-mediated Aβ aggregation inhibitory activity, chelated biometals and reduced ROS production were found in compound a8. In the bi-directional transport assay, a8 exhibited a blood-brain barrier penetrating ability. In this study, the Morris water maze task showed that compound a8 significantly improved the learning and memory impairment of the scopolamine-induced AD mice model. Results highlighted the potential of compound a8 to be a potential MTDL for the development of anti-AD agents.
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http://dx.doi.org/10.1016/j.cbi.2021.109489DOI Listing
June 2021
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