Publications by authors named "Li Yuan"

3,997 Publications

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Screen-printed analytical strip constructed with bacteria-templated porous N-doped carbon nanorods/Au nanoparticles for sensitive electrochemical detection of dopamine molecules.

Biosens Bioelectron 2021 May 6;186:113303. Epub 2021 May 6.

Institute of Materials Science and Devices, School of Materials Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215011, China; Institute for Clean Energy and Advanced Materials, Southwest University, Chongqin, 400715, China; Institute of Advanced Cross-field Science, Qingdao University, Qingdao, 266071, PR China. Electronic address:

Dopamine (DA) as an important neurotransmitter plays an important role in physiological activities, and its abnormal level can cause diseases such as Parkinson's disease. However, the clinical analysis of DA mainly relies on time-consuming and expensive liquid chromatography and molecular spectrometer. We present here a design and fabrication of inexpensive strip sensor constructed from screen printed electrodes for sensitive and selective detection of DA. The ink used for printing the strips contains Shewanella putrefaciens-templated porous N-doped carbon nanorods (N-doped CN) and Au nanoparticles (Au NPs), in which the N-doping enhances CN's negative charge to electrostatically attract the positively charged DA with strong adsorption for achieving fast electron transfer. Moreover, results indicate that the Au NPs impregnation in N-doped CN renders much more catalytic reaction sites toward DA oxidation current. The strip sensor exhibits high sensitivity for DA detection with a broad linear range of 0.02-700 μM and a low detection limit of 0.007 μM as well as good selectivity and superior flexibility for great potential in wearable applications. The strip sensor further performs an accurate detection of DA in human serum, providing a powerful analytical tool for diagnosis of DA related diseases in clinical analysis.
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http://dx.doi.org/10.1016/j.bios.2021.113303DOI Listing
May 2021

Identifying Genomic Alterations in Small Cell Lung Cancer Using the Liquid Biopsy of Bronchial Washing Fluid.

Front Oncol 2021 26;11:647216. Epub 2021 Apr 26.

Department of Respiratory Ward One, Shanxi Provincial Cancer Hospital, Taiyuan, China.

With the rapid development of cancer genomics and immunomics, some new treatments of small cell lung cancer (SCLC) are emerging. However, there are limitations to the clinical use of tumor tissue. Our study aimed to evaluate the potential use of bronchial washing fluid (BWF) in the liquid biopsy of SCLC. Twenty-one extensive SCLC (ES-SCLC) patients were enrolled in this study. For all patients, four sample types, BWF supernatant (BWFs), BWF precipitate (BWFp), plasma and tumor tissue, were collected before receiving chemotherapy, and one type, plasma, was collected after chemotherapy. All samples were conducted to NGS using the 1021-gene panel. The concordance rates of genomic profiling using NGS in the four types of samples were evaluated. Multiple clinical information was analyzed for correlation. We successfully tested 20 BWFs samples, 21 BWFp samples, 21 tumor tissue samples, 20 pre-treatment plasma, and 13 post-treatment plasma of these 21 patients. The detectability of somatic mutations was 100% for BWFs, BWFp, tumor tissues, and post-treatment plasma, and only one pre-treatment plasma was absent with any mutation. Matched tumor tissue, BWFs, BWFp, and pre-treatment plasma samples were subsistent for 19 patients. For these patients, 204 genomic alterations were identified in tissue samples, while 189 (92.6%), 175 (85.5%), and 163 (79.9%) alterations were detected in the matched BWFs, BWFp, and pre-treatment plasma, respectively. Moreover, we found that the three tumor markers associated with SCLC have a lower sensitivity than genomic alterations. The endocrine resistance pathway was found enriched in hyponatremia patients which may be related to the hyponatremia. The TMBs of BWF, BWFp, and pre-treatment plasma samples all had a strong correlation with that of tissue samples. Both the VAF and the MVAF of mutations in post-treatment plasma were less than those in pre-treatment plasma, which was in accordance with the evaluation of curative effect. For ES-SCLC patients, the liquid biopsy of BWF showed a highly potential advantage to identify DNA alterations, which suggested that genomic analysis of BWF liquid biopsy may have clinical value as a supplement for tissue and blood detection. Through the restricted validation, it can be widely used in routine clinical practice.
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http://dx.doi.org/10.3389/fonc.2021.647216DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110515PMC
April 2021

Association of thyroid disorders with gestational diabetes mellitus: a meta-analysis.

Endocrine 2021 May 13. Epub 2021 May 13.

Department of Endocrinology, The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Beijing Hospital, National Center of Gerontology, National Health Commission, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, PR China.

Purpose: The current meta-analysis aimed to evaluate the association of thyroid dysfunction and autoimmunity with gestational diabetes mellitus (GDM).

Methods: A comprehensive search from PubMed, Embase, MEDLINE, and Cochrane databases until November 2020 was conducted. Fixed-effect model was used to combine the results when I was <50%. Random-effect model was used to summarize the results when I was >50%.

Results: A total of 44 studies were included in the meta-analysis. Low FT4 levels were closely related with GDM in the first and second trimesters of gestation. Hypothyroxinemia (OR: 1.45; 95% CI: 1.25, 1.68; P < 0.00001), overt (OR: 1.80; 95% CI: 1.73, 1.86; P < 0.00001), and subclinical (OR: 1.54; 95% CI: 1.03, 2.30; P = 0.03) hypothyroidism, overt hyperthyroidism (OR: 1.49; 95% CI: 1.09, 2.04; P = 0.01), and positive thyroid antibodies (OR: 1.49; 95% CI: 1.07, 2.07; P < 0.00001) were observed significantly associated with the risk of GDM. Pregnant women with subclinical hyperthyroidism were less likely to develop GDM (OR: 0.62; 95% CI: 0.39, 0.97; P = 0.04).

Conclusions: Thyroid dysfunction and positive thyroid antibodies were associated with the risk of GDM. Our findings suggest that pregnant women with these thyroid diseases may be offered screening for GDM comprehensively.
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http://dx.doi.org/10.1007/s12020-021-02712-2DOI Listing
May 2021

Glycosylation of Anti-Thyroglobulin IgG1 and IgG4 Subclasses in Thyroid Diseases.

Eur Thyroid J 2021 Apr 27;10(2):114-124. Epub 2020 Aug 27.

Department of Endocrinology, Peking University First Hospital, Beijing, China.

Objective: Thyroglobulin antibodies (TgAb), principally comprising immunoglobulin G (IgG), are frequently found in healthy individuals. Previously, we showed that the glycosylation levels of TgAb IgG differed across various thyroid diseases, suggesting an important role of glycosylation on antibodies in the pathogenesis of thyroid diseases. Since IgG1 and IgG4 are the primary TgAb IgG subclasses, this study aimed to investigate the glycosylation of TgAb IgG1 and IgG4 subclasses in thyroid diseases.

Methods: TgAb IgG was purified by affinity chromatography from the serum of patients with Hashimoto's thyroiditis (HT) ( = 16), Graves' disease (GD) ( = 8), papillary thyroid carcinoma (PTC) ( = 6), and PTC with histological lymphocytic thyroiditis (PTC-T) ( = 9) as well as healthy donors ( = 10). TgAb IgG1 and IgG4 concentrations were determined by enzyme-linked immunosorbent assay, and a lectin microassay was used to assess TgAb IgG1 and IgG4 glycosylation.

Results: Significantly elevated mannose, sialic acid, and galactose levels on TgAb IgG1 were found in HT and PTC patients compared to GD patients and healthy controls (all < 0.05). The mannose, sialic acid, and core fucose levels on TgAb IgG1 in PTC-T patients were higher than in healthy controls (all < 0.05). Additionally, TgAb IgG1 from PTC-T patients exhibited lower sialylation than that from patients with PTC and higher fucosylation than that from patients with HT (both < 0.05). However, TgAb IgG4 glycosylation did not differ among the five groups ( < 0.05).

Conclusion: Our study describes different distributions of TgAb IgG1 glycosylation in various thyroid diseases. The aberrantly increased glycosylation levels of TgAb IgG1 observed in HT, PTC, and PTC-T might be indicative of immune disorders and participate in the pathogenesis of these diseases.
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http://dx.doi.org/10.1159/000507699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8077608PMC
April 2021

Impairments in brain perfusion, executive control network, topological characteristics, and neurocognition in adult patients with asymptomatic Moyamoya disease.

BMC Neurosci 2021 May 12;22(1):35. Epub 2021 May 12.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No. 119 South 4th Ring West Road, Fengtai District, Beijing, 100070, People's Republic of China.

Background: Asymptomatic Moyamoya disease (MMD) impairs hemodynamic and cognitive function. The relationship between these changes, cerebral blood flow (CBF), and network connectivity remains largely unknown. The aim of this study was to increase understanding of the relationship between CBF, functional networks, and neurocognition in adults with asymptomatic MMD. We compared CBF and functional status in 26 patients with MMD and 20 healthy controls using arterial spin labeling and resting state functional magnetic resonance imaging sequences. At the same time, a detailed cognitive test was performed in 15 patients with no cerebral or lumen infarction who were selected by magnetic resonance imaging-T2 FLAIR screening.

Results: Compared to the controls, the patients showed varying degrees of decline in their computational ability (simple subtraction, p = 0.009; complex subtraction, p = 0.006) and short-term memory (p = 0.042). The asymptomatic MMD group also showed decreased CBF in the left anterior central and left inferior frontal gyri of the island flap with multiple node abnormalities in the brain network and reduced network connectivity. There was a significant association of these changes with cognitive decline in the MMD group.

Conclusions: In patients with asymptomatic MMD, disturbance of CBF and impaired brain network connections may be important causes of cognitive decline and appear before clinical symptoms. Clinical trial registration-URL: http://www.chictr.org.cn Unique identifier: ChiCTR1900023610.
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http://dx.doi.org/10.1186/s12868-021-00638-zDOI Listing
May 2021

Correction to "A Universal Fe/N Incorporated Graphdiyne for Printing Flexible Ferromagnetic Semiconducting Electronics".

J Phys Chem Lett 2021 May 12:4706. Epub 2021 May 12.

Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, P.R. China.

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http://dx.doi.org/10.1021/acs.jpclett.1c01477DOI Listing
May 2021

Ketone Body 3-Hydroxybutyrate Ameliorates Atherosclerosis via Receptor Gpr109a-Mediated Calcium Influx.

Adv Sci (Weinh) 2021 05 1;8(9):2003410. Epub 2021 Mar 1.

School of Life Sciences Tsinghua University Beijing 100084 P. R. China.

Atherosclerosis is a chronic inflammatory disease that can cause acute cardiovascular events. Activation of the NOD-like receptor family, pyrin domain containing protein 3 (NLRP3) inflammasome enhances atherogenesis, which links lipid metabolism to sterile inflammation. This study examines the impact of an endogenous metabolite, namely ketone body 3-hydroxybutyrate (3-HB), on a mouse model of atherosclerosis. It is found that daily oral administration of 3-HB can significantly ameliorate atherosclerosis. Mechanistically, 3-HB is found to reduce the M1 macrophage proportion and promote cholesterol efflux by acting on macrophages through its receptor G-protein-coupled receptor 109a (Gpr109a). 3-HB-Gpr109a signaling promotes extracellular calcium (Ca) influx. The elevation of intracellular Ca level reduces the release of Ca from the endothelium reticulum (ER) to mitochondria, thus inhibits ER stress triggered by ER Ca store depletion. As NLRP3 inflammasome can be activated by ER stress, 3-HB can inhibit the activation of NLRP3 inflammasome, which triggers the increase of M1 macrophage proportion and the inhibition of cholesterol efflux. It is concluded that daily nutritional supplementation of 3-HB attenuates atherosclerosis in mice.
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http://dx.doi.org/10.1002/advs.202003410DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097358PMC
May 2021

The prediction of survival in Gastric Cancer based on a Robust 13-Gene Signature.

J Cancer 2021 12;12(11):3344-3353. Epub 2021 Apr 12.

Department of General Surgery, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Gastric cancer represents a major public health problem. Owing to the great heterogeneity of GC, conventional clinical characteristics are limited in the accurate prediction of individual outcomes and survival. This study aimed to establish a robust gene signature to predict the prognosis of GC based on multiple datasets. Initially, we downloaded raw data from four independent datasets of The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO), and performed univariate Cox proportional hazards regression analysis to identify prognostic genes associated with overall survival (OS) from each dataset. Thirteen common genes from four datasets were screened as candidate prognostic signatures. Then, a risk score model was developed based on this 13‑gene signature and validated by four independent datasets and the entire cohort. Patients with a high-risk score had poorer OS and recurrence-free survival (RFS). Multivariate regression and stratified analysis revealed that the 13-gene signature was not only an independent predictive factor but also associated with recurrence when adjusting for other clinical factors. Furthermore, in the high-risk group, gene set enrichment analysis (GSEA) showed that the mTOR signaling pathway and MAPK signaling pathway were significantly enriched. The present study provided a robust and reliable gene signature for prognostic prediction of both OS and RFS of patients with GC, which may be useful for delivering individualized management of patients.
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http://dx.doi.org/10.7150/jca.49658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100809PMC
April 2021

Genome wide analysis of kinesin gene family in Citrullus lanatus reveals an essential role in early fruit development.

BMC Plant Biol 2021 May 10;21(1):210. Epub 2021 May 10.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Background: Kinesin (KIN) as a motor protein is a versatile nano-machine and involved in diverse essential processes in plant growth and development. However, the kinesin gene family has not been identified in watermelon, a valued and nutritious fruit, and yet their functions have not been characterized. Especially, their involvement in early fruit development, which directly determines the size, shape, yield and quality of the watermelon fruit, remains unclear.

Results: In this study, we performed a whole-genome investigation and comprehensive analysis of kinesin genes in C. lanatus. In total, 48 kinesins were identified and categorized into 10 kinesin subfamilies groups based on phylogenetic analysis. Their uneven distribution on 11 chromosomes was revealed by distribution analysis. Conserved motif analysis showed that the ATP-binding motif of kinesins was conserved within all subfamilies, but not the microtubule-binding motif. 10 segmental duplication pairs genes were detected by the syntenic and phylogenetic approaches, which showed the expansion of the kinesin gene family in C. lanatus genome during evolution. Moreover, 5 ClKINs genes are specifically and abundantly expressed in early fruit developmental stages according to comprehensive expression profile analysis, implying their critical regulatory roles during early fruit development. Our data also demonstrated that the majority of kinesin genes were responsive to plant hormones, revealing their potential involvement in the signaling pathways of plant hormones.

Conclusions: Kinesin gene family in watermelon was comprehensively analyzed in this study, which establishes a foundation for further functional investigation of C. lanatus kinesin genes and provides novel insights into their biological functions. In addition, these results also provide useful information for understanding the relationship between plant hormone and kinesin genes in C. lanatus.
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http://dx.doi.org/10.1186/s12870-021-02988-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108342PMC
May 2021

Integration and interplay of machine learning and bioinformatics approach to identify genetic interaction related to ovarian cancer chemoresistance.

Brief Bioinform 2021 May 10. Epub 2021 May 10.

School of Public health and Community Medicine, University of New South Wales, 2052, Sydney, Australia.

Although chemotherapy is the first-line treatment for ovarian cancer (OCa) patients, chemoresistance (CR) decreases their progression-free survival. This paper investigates the genetic interaction (GI) related to OCa-CR. To decrease the complexity of establishing gene networks, individual signature genes related to OCa-CR are identified using a gradient boosting decision tree algorithm. Additionally, the genetic interaction coefficient (GIC) is proposed to measure the correlation of two signature genes quantitatively and explain their joint influence on OCa-CR. Gene pair that possesses high GIC is identified as signature pair. A total of 24 signature gene pairs are selected that include 10 individual signature genes and the influence of signature gene pairs on OCa-CR is explored. Finally, a signature gene pair-based prediction of OCa-CR is identified. The area under curve (AUC) is a widely used performance measure for machine learning prediction. The AUC of signature gene pair reaches 0.9658, whereas the AUC of individual signature gene-based prediction is 0.6823 only. The identified signature gene pairs not only build an efficient GI network of OCa-CR but also provide an interesting way for OCa-CR prediction. This improvement shows that our proposed method is a useful tool to investigate GI related to OCa-CR.
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http://dx.doi.org/10.1093/bib/bbab100DOI Listing
May 2021

Low Serum LH Levels During Ovarian Stimulation With GnRH Antagonist Protocol Decrease the Live Birth Rate After Fresh Embryo Transfers but Have No Impact in Freeze-All Cycles.

Front Endocrinol (Lausanne) 2021 23;12:640047. Epub 2021 Apr 23.

Medical Center for Human Reproduction, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

Objective: To explore the association between serum LH levels and the cumulative live birth rate (CLBR) within one complete cycle, and the impact of serum LH levels on the live birth rate (LBR) after the initial embryo transfer (ET) considering different ET strategies (fresh or freeze-all).

Design: A retrospective cohort study.

Setting: University-affiliated reproductive center.

Patients: 1480 normogonadotrophic women who underwent COS with GnRH antagonist protocol for the first IVF/ICSI attempt.

Interventions: The sample was stratified into low and higher LH groups according to serum LH peak levels of <4 (Group A) and ≥4 IU/L (Group B) during COS. Patients were also sub-grouped into conventional fresh/frozen ET cycles and freeze-all cycles.

Main Outcome Measures: The LBR after the initial embryo transfer and the CLBR within one complete cycle.

Secondary Outcome Measures: The numbers of day-3 high-quality embryos, the numbers of embryos available, and the other pregnancy outcomes after the initial ET.

Results: In the whole cohort, the CLBRs decreased significantly in the low (63.1% vs. 68.3%, =.034) LH group compared to the higher LH group. Subgroup analysis revealed that patients with low LH levels had lower LBR after fresh ET (38.0% vs. 51.5%, =.005) but comparable LBR after the first frozen-thawed ET (FET) in freeze-all cycles (49.8% vs. 51.8%, =.517) than patients with higher LH peak levels. Likewise, patients with low LH levels had lower CLBR for conventional fresh/frozen ET cycles (54.8% vs. 66.1%, =.015) but comparable CLBR for the freeze-all cycles (66.8% vs. 69.2%, =.414) than those with higher LH levels. Following confounder adjustment, multivariable regression analyses showed that low LH level was an independent risk factor for the CLBR in the whole cohort (odds ratio (OR): 0.756, 95% confidence interval (CI): 0.604-0.965, =.014) and in patients who underwent the conventional ET strategy (OR: 0.596, 95% CI: 0.408-0.917, =.017). Moreover, the adverse impact of low LH levels on LBRs maintained statistically significant after fresh transfers (OR: 0.532, 95% CI: 0.353-0.800, =.002) but not after the first FETs in freeze-all cycles (OR: 0.918, 95% CI: 0.711-1.183,=.508).

Conclusions: In comparison with higher LH levels, low LH levels decrease the CLBRs per oocyte retrieval cycle for normogonadotrophic women who underwent COS using GnRH antagonists. This discrepancy may arise due to the significant detrimental effect of low LH levels on the LBRs after fresh embryo transfers.
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http://dx.doi.org/10.3389/fendo.2021.640047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104121PMC
April 2021

Aberrant hypermethylation induced downregulation of antisense lncRNA STXBP5-AS1 and its sense gene STXBP5 correlate with tumorigenesis of glioma.

Life Sci 2021 May 6;278:119590. Epub 2021 May 6.

Department of Laboratory Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250000, China. Electronic address:

Aims: The expression of antisense lncRNA STXBP5-AS1 and its sense gene STXBP5 were found to be downregulated in glioma by RNA sequencing; however, the function and mechanism of both two genes in the development of glioma have not been studied.

Materials And Methods: QRT-PCR and western blot were used to determine the transcriptional and translational levels of moleculars. MSP and BSP assays were used to evaluate the methylation status of promoter CpG island. MTT, EdU, flow cytometry, and transwell assays were used to reveal biological effects. The in vivo mice model was used to validate the role of target genes in tumorigenesis.

Key Findings: The mRNA and protein expression of STXBP5 was significantly downregulated in glioma tissues and positively correlated with prognosis. STXBP5-AS1 was downregulated in glioma cells and tissues, and associated with tumor size and clinical stages. Both of two genes were significantly restored in cells treatment with 5-Aza. The promoter CpG island of STXBP5/STXBP5-AS1 was hypermethylated in glioma cells, but partially methylated in NHA cells. We found that promoter methylation frequency was significantly higher in glioma tissues. Functionally, overexpression of STXBP5 and STXBP5-AS1 inhibited cell proliferation, migration, and invasion and promoted apoptosis in vitro, whereas depletion of STXBP5 and STXBP5-AS1 showed opposite effects. Both the mRNA and protein expression of STXBP5 were positively regulated by STXBP5-AS1. Ectopic expression of STXBP5 and STXBP5-AS1 suppressed tumor formation in vivo.

Significance: Our findings suggested that epigenetically silenced STXBP5-AS1 and STXBP5 might act as novel tumor suppressors of glioma.
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http://dx.doi.org/10.1016/j.lfs.2021.119590DOI Listing
May 2021

Ultrasensitive ratiometric electrochemiluminescence for detecting atxA mRNA using luminol-encapsulated liposome as effectively amplified signal labels.

Biosens Bioelectron 2021 May 1;186:113263. Epub 2021 May 1.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China. Electronic address:

It is an advantageous way to quickly identify the toxicity of Bacillus anthracis (B. anthracis) by detecting the transcription product of the atxA gene. Herein, a novel ultrasensitive ratiometric electrochemiluminescence (ECL) biosensor with competitive mechanism and double amplified signal ways was proposed for detecting the atxA mRNA. The KSO was used as cathodic emitter and silver metal-organic gels (AgMOG) was used as ECL enhancer. The AgMOG could accelerate the electro-catalytic reduction of SO to SO˙-, which reacted with dissolved oxygen, resulting in strong cathodic ECL. Meanwhile, luminol was encapsulated in liposome as anodic amplified signal labels and the luminol anion radical also reacted with dissolved oxygen to create the anodic ECL emission. We immobilized luminol-encapsulated liposomes on the surface of AgMOG through the hybridization of DNA and mRNA. This would provide a competitive mechanism involving dissolved oxygen between KSO and luminol. Benefiting from the competitive mechanism and amplified signal ways, this ratiometric biosensor achieved a wide linear relationship range from 10 to 300 fM with a low limit of detection (8.13 fM). Considering the accessible operation, favorable performance, and high universality of this strategy, this work may be used to analyze other mRNAs of bacteria.
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http://dx.doi.org/10.1016/j.bios.2021.113263DOI Listing
May 2021

Intestinal mucosa-derived DNA methylation signatures in the penetrating intestinal mucosal lesions of Crohn's disease.

Sci Rep 2021 May 7;11(1):9771. Epub 2021 May 7.

Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, China.

The purpose of this study was to evaluate genome-wide DNA methylation changes in intestinal mucosa tissue of adult patients with Crohn's disease comprehensively. DNA methylation chip was used to analyze abnormal methylation sites among penetrating and non-penetrating intestinal mucosa tissue of Crohn's disease and normal intestinal mucosa tissue of healthy controls. Methylation abnormalities of different locus were verified by pyrosequencing and quantitative polymerase chain reaction. Differential DNA methylation sites were participated in the positive regulation of apoptosis and the positive regulation of IL-8 production and were enriched in signaling pathways related to inflammatory bowel disease and extracellular matrix receptor interaction signaling pathways. Correlation analysis showed that the methylation abnormalities of HLA-DRB1 (r = - 0.62, P < 0.001), MUC1 (r = - 0.45, P = 0.01), YPEL5 (r = - 0.55, P = 0.001) and CBLB (r = - 0.62, P < 0.001) were significantly negatively correlated with their relative expression levels. The degree of methylation abnormality of MUC1 was negatively correlated with the disease activity score of Crohn's disease (r = - 0.50, P = 0.01). Apoptosis, interleukin-8 production and abnormal extracellular matrix might be involved in the mechanism of penetrating intestinal mucosal lesions in Crohn's disease. The degree of abnormal methylation of MUC1 was negatively correlated with the disease activity of Crohn's disease.
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http://dx.doi.org/10.1038/s41598-021-89087-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105344PMC
May 2021

Reck-Notch1 Signaling Mediates miR-221/222 Regulation of Lung Cancer Stem Cells in NSCLC.

Front Cell Dev Biol 2021 20;9:663279. Epub 2021 Apr 20.

Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Cancer stem cells (CSCs) contribute to the cancer initiation, metastasis and drug resistance in non-small cell lung cancer (NSCLC). Herein, we identified a miR-221/222 cluster as a novel regulator of CSCs in NSCLC. Targeted overexpression or knockdown of miR-221/222 in NSCLC cells revealed the essential roles of miR-221/222 in regulation of lung cancer cell proliferation, mammosphere formation, subpopulation of CD133 CSCs and the expression of stemness genes including OCT4, NANOG and h-TERT. The animal study showed that overexpression of miR-221/222 significantly enhanced the capacity of lung cancer cells to develop tumor and grow faster, indicating the importance of miR-221/222 in tumorigenesis and tumor growth. Mechanistically, Reck was found to be a key direct target gene of miR-221/222 in NSCLC. Overexpression of miR-221/222 significantly suppressed Reck expression, activated Notch1 signaling and increased the level of NICD. As an activated form of Notch1, NICD leads to enhanced stemness in NSCLC cells. In addition, knockdown of Reck by siRNA not only mimicked miR-221/222 effects, but also demonstrated involvement of Reck in the miR-221/222-induced activation of Notch1 signaling, verifying the essential roles of the miR-221/222-Reck-Notch1 axis in regulating stemness of NSCLC cells. These findings uncover a novel mechanism by which lung CSCs are significantly manipulated by miR-221/222, and provide a potential therapeutic target for the treatment of NSCLC.
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http://dx.doi.org/10.3389/fcell.2021.663279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093830PMC
April 2021

Effects of soil type, moisture content and organic amendment rate on dimethyl disulfide distribution and persistency in soil.

Environ Pollut 2021 Apr 30;285:117198. Epub 2021 Apr 30.

State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Electronic address:

Understanding the distribution and persistence of the fumigant dimethyl disulfide (DMDS) under different soil conditions would contribute to a more environmentally sustainable use of this gas. We determined the effects of soil type, soil moisture content and soil organic amendment rate on DMDS distribution and persistency using soil columns in the laboratory. The peak concentrations of DMDS at 60 cm soil depth in sandy loam soil, black soil and red loam soil were 1.9 μg cm, 0.77 μg cm, 0.22 μg cm, respectively. The total soil residues of DMDS in sandy loam soil, black soil and red loam soil were 0.4, 1.3 and 1.3%, respectively. The peak concentrations of DMDS at 60 cm soil depth and the total soil residues of DMDS applied decreased from 3.2 μg cm to 0.9 μg cm and 3.3 to 0.5% when soil moisture content increased from 6 to 18%, respectively. Incremental increases (0-5%) in organic amendment rates decreased DMDS distribution through the soils and increased soil residues. Wait periods were required of 7, 21 and 21 days after polyethylene (PE) film was removed to reduce residues sufficiently for cucumber seed germination in sandy loam soil, black soil and red loam soil with 12% moisture content and 0% organic amendment rate, respectively. However, no wait period was required for successful cucumber seed germination in sandy loam soils (Beijing) with 6, 12 or 18% moisture content or organic amendment rates of 1 or 5%, respectively, but in commercial practice 7 days delay would be prudent. Our results indicated that soil type, soil moisture content and organic amendment rates significantly affected DMDS distribution, persistency and residues in soil. Those factors should be taken into consideration by farmers when determining the appropriate dose of DMDS that will control soil pests and diseases in commercially-produced crops.
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http://dx.doi.org/10.1016/j.envpol.2021.117198DOI Listing
April 2021

Manual Acupuncture or Combination with Vitamin B to Treat Diabetic Peripheral Neuropathy: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Biomed Res Int 2020 21;2020:4809125. Epub 2020 Nov 21.

First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.

Methods: Randomized controlled trials on manual acupuncture treatment of DPN were retrieved from the Medline, Web of Science, PubMed, Cochrane Library, EMBASE, CNKI, WanFang, and VIP databases. Extracted research data were summarized in the tables, and methodological assessment was performed using the risk-of-bias assessment tool of Cochrane. Meta-analysis was performed by Revman 5.3, Stata 14.0, and TSA 0.9.5.10 Beta software.

Results: A total of 18 randomized clinical trials (RCTs) were recruited: (1) 11 RCTs were acupuncture alone compared with vitamin B; (2) 7 RCTs were acupuncture combined with vitamin B compared with vitamin B, involving 1200 participants. Acupuncture alone improved clinical efficacy ( < 0.05) and nerve conduction velocity of the four peripheral nerves: peroneal nerve, tibial nerve, median nerve, and ulnar nerve ( < 0.05), but there was no significant difference between the group of acupuncture alone and the group of vitamin B ( = 0.36 > 0.05) in improving median nerve SCV (sensory nerve conduction velocity). Acupuncture combined with vitamin B improved clinical efficacy and nerve conduction velocity of the three peripheral nerves, peroneal nerve, tibial nerve, and median nerve ( < 0.05), and decreased the scores of the Toronto clinical scoring system (TCSS) ( < 0.05).

Conclusion: Acupuncture alone and vitamin B combined with acupuncture are more effective in treating DPN compared to vitamin B. However, more high-quality RCTs on vitamin B combined with acupuncture are required to confirm our results.
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http://dx.doi.org/10.1155/2020/4809125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067773PMC
November 2020

Primary Tumor Resection Improves Survival for EGFR-TKI-Treated Patients With Occult M1a Lung Adenocarcinoma.

Front Oncol 2021 19;11:622723. Epub 2021 Apr 19.

Department of Thoracic Surgery and State Key Laboratory of Genetic Engineering, Fudan University Shanghai Cancer Center, Shanghai, China.

Background: The role of primary tumor resection in occult M1a lung adenocarcinoma remains unclear, especially for patients receiving targeted therapy. The purpose of this study is to assess the effect of primary tumor resection on overall survival (OS) in lung adenocarcinoma patients with occult pleural disseminations receiving targeted therapy.

Methods: Lung adenocarcinoma patients with intraoperatively-confirmed occult pleural dissemination (M1a), who hospitalized in the Department of Thoracic Surgery in Fudan Shanghai Cancer Center from May 2008 to December 2017 and received EGFR-TKIs therapy, were enrolled. Log-rank tests were used to compare the survival differences between groups.

Results: 34 patients receiving EGFR-TKIs were enrolled. The majority of them were never smokers (29/34, 85.3%). Among the enrolled patients, 20 (58.8%) patients underwent primary tumor resection, while 14 (41.2%) patients not. There was no distributional difference of baselines between patients undergoing and not undergoing primary tumor resection. Further analyses demonstrated that the patients undergoing primary tumor resection had a prolonged OS compared with those not (log-rank = 0.042). The 2-year and 5-year OS for patients receiving primary tumor resection and EGFR-TKIs was 90.0% and 60.1%.

Conclusions: Primary tumor resection was associated with improved survival in patients with occult intraoperatively-confirmed M1a adenocarcinoma receiving EGFR-TKIs.
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http://dx.doi.org/10.3389/fonc.2021.622723DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092396PMC
April 2021

2-Hydroxypropyl-β-cyclodextrin encapsulates dimethyl disulfide producing a controlled release formulation.

Sci Total Environ 2021 Jun 22;773:145293. Epub 2021 Jan 22.

Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address:

Dimethyl disulfide (DMDS), a soil fumigant, is an effective, broad-spectrum compound that often replaces bromomethane (MB) in the prevention and treatment of soil-borne diseases. However, the disadvantages of DMDS include toxicity, volatility, pungent odor, risk of human exposure, and environmental pollution. Cyclodextrin (CD) has been widely used as a carrier of chemicals in many industries due to its functional advantages and safety. In this study, a DMDS-controlled release formulation was developed by encapsulating DMDS in the cavity of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD). This formulation reduced DMDS usage and production costs. Orthogonal experimental design, Fourier transform infrared (FT-IR), Scanning electron microscopy (SEM), Thermal gravity analysis (TGA) characterization, efficacy comparison, safety, and other aspects of the evaluation showed that under the best preparation conditions, the encapsulation rate was 81.49%. The efficacy of DMDS@HP-β-CD was similar to unformulated DMDS. The efficacy duration of the formulation was about two times longer than DMDS, and it was safer to use. This study reveals a cyclodextrin-DMDS formulation with reduced toxicity, longer duration, environmental safety and sustainability.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145293DOI Listing
June 2021

Health risks from groundwater arsenic on residents in northern China coal-rich region.

Sci Total Environ 2021 Jun 2;773:145003. Epub 2021 Feb 2.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address:

Shanxi Province of northern China is a typical mining concentration and arsenism area. Years of mining activities have resulted in serious regional groundwater problems in Shanxi. Therefore, it is of great significance to know the health risk of groundwater arsenic on residents under the background of mining activities. Kriging interpolation was used to illustrate the spatio-temporal dynamics of the health risks on groundwater arsenic based on a ten-year investigation. The groundwater arsenic concentrations decreased over time and the distribution of high arsenic concentrations shrank. High arsenic concentrations were mainly distributed in the northern and middle basin areas. The forecasted area of high risks in coal mining areas was 5623 km, which was larger than that in non-coal mining areas. The residents living around mining areas were more vulnerable to exposure to groundwater arsenic. Further, the output map outlines the high-risk zones in order to protect the safety of drinking water for residents. This study may be helpful for the policy-makers to adopt a lower limit for groundwater arsenic to the worst affected regions and groups.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145003DOI Listing
June 2021

Detection of Motor Dysfunction With Wearable Sensors in Patients With Idiopathic Rapid Eye Movement Disorder.

Front Bioeng Biotechnol 2021 15;9:627481. Epub 2021 Apr 15.

Department of Neurobiology, Neurology and Geriatrics, Xuanwu Hospital of Capital Medical University, Beijing Institute of Geriatrics, Beijing, China.

Patients with idiopathic rapid eye movement sleep behavior disorder (iRBD) are at high risk for conversion to synucleinopathy and Parkinson disease (PD). This can potentially be monitored by measuring gait characteristics of iRBD patients, although quantitative data are scarce and previous studies have reported inconsistent findings. This study investigated subclinical gait changes in polysomnography-proven iRBD patients compared to healthy controls (HCs) during 3 different walking conditions using wearable motor sensors in order to determine whether gait changes can be detected in iRBD patients that could reflect early symptoms of movement disorder. A total 31 iRBD patients and 20 HCs were asked to walk in a 10-m corridor at their usual pace, their fastest pace, and a normal pace while performing an arithmetic operation (dual-task condition) for 1 min each while using a wearable gait analysis system. General gait measurements including stride length, stride velocity, stride time, gait length asymmetry, and gait variability did not differ between iRBD patients and HCs; however, the patients showed decreases in range of motion ( = 0.004) and peak angular velocity of the trunk ( = 0.001) that were significant in all 3 walking conditions. iRBD patients also had a longer step time before turning compared to HCs ( = 0.035), and the difference between groups remained significant after adjusting for age, sex, and height. The decreased trunk motion while walking and increased step time before turning observed in iRBD may be early manifestations of body rigidity and freezing of gait and are possible prodromal symptoms of PD.
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http://dx.doi.org/10.3389/fbioe.2021.627481DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084288PMC
April 2021

p16 loss facilitate hydroquinone-induced malignant transformation of TK6 cells through promoting cell proliferation and accelerating the cell cycle progression.

Environ Toxicol 2021 May 1. Epub 2021 May 1.

Department of Environmental and Occupational Health, Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, China.

The p16 is a multifunction gene that includes regulation of the cell cycle, apoptosis, senescence and tumor development. However, the effects of p16 in hydroquinone-induced malignant transformation of TK6 cells remain unclear. The present study aimed to explore whether p16 loss facilitate malignant transformation in TK6 cells. The results demonstrated that p16/Rb signal pathway was suppressed in hydroquinone-induced malignant transformation of TK6 cells. We further confirmed that p16 loss stimulated cell proliferation, and accelerated cell cycle progression in vitro and in vivo. The immunoblotting analysis indicated that p16 regulated cell cycle progression via Rb and p53. Therefore, we conclude that p16 is involved in HQ-induced malignant transformation associated with suppressing Rb and p53 which resulting in accelerating the cell cycle progression.
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http://dx.doi.org/10.1002/tox.23155DOI Listing
May 2021

Isolation, culture, and characterization of cells derived from giant panda (Ailuropoda melanoleuca) semen.

In Vitro Cell Dev Biol Anim 2021 Apr 29. Epub 2021 Apr 29.

Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Province, Chengdu, 610081, Sichuan, China.

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http://dx.doi.org/10.1007/s11626-021-00579-1DOI Listing
April 2021

Graphene-Reinforced Titanium Enhances Soft Tissue Seal.

Front Bioeng Biotechnol 2021 13;9:665305. Epub 2021 Apr 13.

Department of Oral and Maxillo-facial Implantology, Shanghai Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

The integrity of soft tissue seal is essential for preventing peri-implant infection, mainly induced by established bacterial biofilms around dental implants. Nowadays, graphene is well-known for its potential in biocompatibility and antisepsis. Herein, a new titanium biomaterial containing graphene (Ti-0.125G) was synthesized using the spark plasma sintering (SPS) technique. After material characteristics detection, the subsequent responses of human gingival fibroblasts (HGFs) and multiple oral pathogens (including , , and ) to the graphene-reinforced sample were assessed, respectively. Also, the dynamic change of the bacterial multispecies volume in biofilms was evaluated using absolute quantification PCR combined with Illumina high-throughput sequencing. Ti-0.125G, in addition to its particularly pronounced inhibitory effect on at 96 h, was broadly effective against multiple pathogens rather than just one strain. The reinforced material's selective responses were also evaluated by a co-culture model involving HGFs and multiple strains. The results disclosed that the graphene-reinforced samples were highly effective in keeping a balance between the favorable fibroblast responses and the suppressive microbial growth, which could account for the optimal soft tissue seal in the oral cavity. Furthermore, the underlying mechanism regarding new material's bactericidal property in the current study has been elucidated as the electron transfer, which disturbed the bacterial respiratory chain and resulted in a decrease of microbial viability. According to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, the PICRUSt tool was conducted for the prediction of microbial metabolism functions. Consequently, it is inferred that Ti-0.125G has promising potentials for application in implant dentistry, especially in enhancing the integrity of soft tissue and improving its resistance against bacterial infections around oral implants.
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http://dx.doi.org/10.3389/fbioe.2021.665305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076685PMC
April 2021

Gut Microbiota in Untreated Diffuse Large B Cell Lymphoma Patients.

Front Microbiol 2021 13;12:646361. Epub 2021 Apr 13.

Department of Hematology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Intestinal microecology plays an important role in the development and progression of hematological malignancies. However, characteristics of gut microbiota in diffuse large B cell lymphoma (DLBCL) have not been reported. The microbiota composition of fecal samples from 25 untreated DLBCL patients and 26 healthy volunteers was examined by 16S rRNA gene sequencing. On α-diversity analysis, there was no significant difference in species diversity and abundance between the two groups. However, a significant difference was observed on β-diversity analysis. The intestinal microbiota in patients with DLBCL showed a continuous evolutionary relationship, which progressed from phylum, , to genus, . Their abundance was significantly higher than that of the control group. At the genus level, , , and were more abundant in patients with DLBCL than in the control group. Functional prediction by PICRUSt indicated that thiamine metabolism and phenylalanine, tyrosine, and tryptophan biosynthesis were significantly lower in the DLBCL group than in the control group. In conclusion, our results clearly demonstrate that the gut microbiota was changed significantly in DLBCL. The study highlights fundamental differences in the microbial diversity and composition of patients with DLBCL and paves the way for future prospective studies and microbiome-directed interventional trials to improve patient outcomes.
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http://dx.doi.org/10.3389/fmicb.2021.646361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076791PMC
April 2021

Molecular Cloning and Functional Analysis of 1-Deoxy-D-Xylulose 5-Phosphate Reductoisomerase from .

Genes (Basel) 2021 Apr 22;12(5). Epub 2021 Apr 22.

Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.

Sandalwood ( L.) heartwood-derived essential oil contains a high content of sesquiterpenoids that are economically highly valued and widely used in the fragrance industry. Sesquiterpenoids are biosynthesized via the mevalonate acid and methylerythritol phosphate (MEP) pathways, which are also the sources of precursors for photosynthetic pigments. 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) is a secondary rate-limiting enzyme in the MEP pathway. In this paper, the 1416-bp open reading frame of and its 897-bp promoter region, which contains putative conserved -elements involved in stress responsiveness (HSE and TC-rich repeats), hormone signaling (abscisic acid, gibberellin and salicylic acid) and light responsiveness, were cloned from 7-year-old trees. A bioinformatics analysis suggested that encodes a functional and conserved DXR protein. was widely expressed in multiple tissues, including roots, twigs, stem sapwood, leaves, flowers, fruit and stem heartwood, displaying significantly higher levels in tissues with photosynthetic pigments, like twigs, leaves and flowers. mRNA expression increased in etiolated seedlings exposed to light, and the content of chlorophylls and carotenoids was enhanced in all transgenic lines, consistent with the expression level. was also stimulated by MeJA and HO in seedling roots. α-Santalol content decreased in response to fosmidomycin, a DXR inhibitor. These results suggest that plays an important role in the biosynthesis of photosynthetic pigments, shifting the flux to sandalwood-specific sesquiterpenoids.
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http://dx.doi.org/10.3390/genes12050626DOI Listing
April 2021

Microgel-Stabilized Hydroxypropyl Methylcellulose and Dextran Water-in-Water Emulsion: Influence of pH, Ionic Strength, and Temperature.

Langmuir 2021 May 29;37(18):5617-5626. Epub 2021 Apr 29.

Department of Nutrition & Food Science, University of Maryland, College Park, Maryland 20742, United States.

A stable water-in-water (W/W) emulsion was formed by mixing dextran and hydroxypropyl methylcellulose (HPMC) with addition of β-lactoglobulin (Blg) microgels. The microstructure and stability of the W/W emulsion were investigated under different conditions. The microgels accumulating at the liquid-liquid interface led to a stable emulsion at pH 3-5, where the microgels carried positive charges. When the pH was increased above the pI of microgels (∼pH 5), the emulsion was destabilized because the microgels tended to stay in the continuous phase (i.e., dextran) rather than at the interface. The HPMC-in-dextran emulsions were stable under ionic strength levels up to 300 mM. The HPMC-in-dextran emulsion stabilized by Blg microgels was thermally stable, and the heat treatment promoted partial Blg microgel particle-particle fusion on the surface of HPMC droplets at 90 °C. Electrostatic and hydrophobic interactions between dextran and HPMC phase were further investigated to understand the microgels' accumulation at the liquid-liquid interface.
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http://dx.doi.org/10.1021/acs.langmuir.1c00484DOI Listing
May 2021

Initiation and amplification of SnRK2 activation in abscisic acid signaling.

Nat Commun 2021 04 28;12(1):2456. Epub 2021 Apr 28.

Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.

The phytohormone abscisic acid (ABA) is crucial for plant responses to environmental challenges. The SNF1-regulated protein kinase 2s (SnRK2s) are key components in ABA-receptor coupled core signaling, and are rapidly phosphorylated and activated by ABA. Recent studies have suggested that Raf-like protein kinases (RAFs) participate in ABA-triggered SnRK2 activation. In vitro kinase assays also suggest the existence of autophosphorylation of SnRK2s. Thus, how SnRK2 kinases are quickly activated during ABA signaling still needs to be clarified. Here, we show that both B2 and B3 RAFs directly phosphorylate SnRK2.6 in the kinase activation loop. This transphosphorylation by RAFs is essential for SnRK2 activation. The activated SnRK2s then intermolecularly trans-phosphorylate other SnRK2s that are not yet activated to amplify the response. High-order Arabidopsis mutants lacking multiple B2 and B3 RAFs show ABA hyposensitivity. Our findings reveal a unique initiation and amplification mechanism of SnRK2 activation in ABA signaling in higher plants.
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http://dx.doi.org/10.1038/s41467-021-22812-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080645PMC
April 2021

Direct visualization of photo-induced disulfide through oxidative coupling of -aminothiophenol.

Chem Commun (Camb) 2021 Apr;57(34):4190-4193

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, P. R. China.

Chemical transformations under visible irradiation are interesting in green preparation. Herein, a photo-oxidative coupling reaction of para-aminothiophenol (p-ATP) dimerizing to 4-aminophenyl disulfide (APDS) rather than 4,4'-dimercaptoazobenzene (DMAB) was achieved in water by visible light irradiation, producing monodispersed organic nanoparticles in situ with strong light scattering visualized by the dark-field microscopy (DFM) imaging technique, owing to the formation of disulfide.
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http://dx.doi.org/10.1039/d1cc00587aDOI Listing
April 2021

The VIM-AS1/miR-655/ZEB1 axis modulates bladder cancer cell metastasis by regulating epithelial-mesenchymal transition.

Cancer Cell Int 2021 Apr 26;21(1):233. Epub 2021 Apr 26.

Department of Urology, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Kaifu District, Changsha, 410008, Hunan, China.

Background: Invasive bladder tumors cause a worse prognosis in patients and remain a clinical challenge. Epithelial-mesenchymal transition (EMT) is associated with bladder cancer metastasis. In the present research, we attempted to demonstrate a novel mechanism by which a long noncoding RNA (lncRNA)-miRNA-mRNA axis regulates EMT and metastasis in bladder cancer.

Methods: Immunofluorescence (IF) staining was used to detect Vimentin expression. The protein expression of ZEB1, Vimentin, E-cadherin, and Snail was investigated by using immunoblotting assays. Transwell assays were performed to detect the invasive capacity of bladder cancer cells. A wound healing assay was used to measure the migratory capacity of bladder cancer cells.

Results: Herein, we identified lncRNA VIM-AS1 as a highly- expressed lncRNA in bladder cancer, especially in metastatic bladder cancer tissues and high-metastatic bladder cancer cell lines. By acting as a ceRNA for miR-655, VIM-AS1 competed with ZEB1 for miR-655 binding, therefore eliminating the miR-655-mediated suppression of ZEB1, finally promoting EMT in both high- and low-metastatic bladder cancer cells and enhancing cancer cell metastasis.

Conclusions: In conclusion, the VIM-AS1/miR-655/ZEB1 axis might be a promising target for improving bladder cancer metastasis via an EMT-related mechanism.
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http://dx.doi.org/10.1186/s12935-021-01841-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074428PMC
April 2021