Publications by authors named "Li Yu"

5,466 Publications

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Predictive Value of Adenoviral Load for Bronchial Mucus Plugs Formation in Children with Adenovirus Pneumonia.

Can Respir J 2022 8;2022:9595184. Epub 2022 Aug 8.

Hunan Provincial Key Laboratory of Pediatric Respirology, Pediatric Medical Center, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha 410000, China.

Background: The study aimed to explore risk factors for bronchial mucus plugs (BMP) formation in children with adenovirus (AdV) pneumonia.

Methods: A retrospective study was conducted on children with AdV pneumonia who underwent bronchoscopy from January 2019 to December 2019. Children were divided into the BMP group and the control group, depending on whether BMP was formed or not. The clinical information and treatment proposals of the two groups of children were counted and analyzed via multiple logistic regression analysis, ROC curve analysis, and correlation analysis.

Results: Among 453 patients with AdV pneumonia, 185 (40.84%) were in the BMP group. Among all the cases, there were 188 patients with a single AdV infection, including 64 (34.04%) in the BMP group and 124 (65.96%) in the control group. The incidence of dyspnea, poor spirits, mixed infections, and other symptoms in the BMP group was higher than in the control group. Children in the BMP group had a longer heat range. C-reactive protein (CRP), lactate dehydrogenase (LDH), D-dimer (DD), and AdV load levels were higher in the MBP group. AdV load, coinfection, DD, heat range, and LDH were independent risk factors for BMP, among which AdV load was the most significant (AUC = 0.819). AdV load was positively correlated with other risk factors, respectively. AdV load and heat range were independent risk factors for BMP patients with a single AdV infection.

Conclusion: AdV load might have important clinical value in predicting BMP development in AdV pneumonia.
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http://dx.doi.org/10.1155/2022/9595184DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377942PMC
August 2022

Paenibacillus alkalitolerans sp. nov., a bacterium isolated from a salt lake of Turpan City in Xinjiang Province, north-west China.

Folia Microbiol (Praha) 2022 Aug 17. Epub 2022 Aug 17.

Key Laboratory for Conservation and Utilization of Bio-Resource, and Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.

A Gram stain-positive, rod-shaped, motile, aerobic and terminal endospore formation bacterium, designated YIM B00362, was isolated from saline soil samples collected from a salt lake in Xinjiang Province, north-west China. Phylogenetic analysis based on the 16S rRNA gene sequences and whole genomes indicated that the isolate belongs to the genus Paenibacillus. However, the highest sequence similarity between strain YIM B00362 and the relatives was only 94.4%. Moreover, the DNA-DNA relatedness and ANI values between the novel isolate and the relative type strain, Paenibacillus antri SYSU K30003 was 13.6% and 70.3%, respectively. The major cellular fatty acids were anteiso-C, C and the major quinone was MK-7. The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylglyceride, and two unidentified polar lipids. The genomic DNA G + C content was 50.9 mol%. The major whole-cell sugars contained glucose and galactose. On the basis of physiological, phenotypic, and chemotaxonomic data, strain YIM B00362 represents a novel species of genus Paenibacillus, for which the name Paenibacillus alkalitolerans sp. nov. is proposed. The type strain is YIM B00362 (= KCTC 43272 = CGMCC 1.18801 = NBRC 114667).
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http://dx.doi.org/10.1007/s12223-021-00931-8DOI Listing
August 2022

Recent advances of three-dimensional micro-environmental constructions on cell-based biosensors and perspectives in food safety.

Biosens Bioelectron 2022 Aug 4;216:114601. Epub 2022 Aug 4.

School of Food Science and Technology, Zhejiang University, Hangzhou, Zhejiang, 310000, People's Republic of China. Electronic address:

The development and application of cell-based biosensors (CBBs) provides a convenient strategy for rapid detection of target analytes. The CBBs had been widely applied in the fields of food safety, environment monitoring, and medicine diagnosis due to their advantages of short response time, easy operation, low toxicity, and portability. However, the CBBs based on two-dimensional (2D) cultured cells in-vitro suffer from a lower cell viability and isolated physiology, which had blocked the accurate evaluations of these biosensors. With the development of nanotechnology and three-dimensional (3D) printing technology, cells fixed in a 3D biosensor or a 3D microenvironment have shown great improvement in the sensitivity and detection authenticity than conventional CBBs. To promote the further development of CBBs, in this paper, we reviewed the related technologies used to construct 3D bionic cell chips including organic/inorganic agents and operating approaches suitable for constructing 3D cell cultural microenvironment. Then, the applications of 3D bionic cell chip based on microbial and mammalian cell biosensors in food safety field were discussed during recent ten years. Finally, the current challenges and further directions were summarized and prospected.
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http://dx.doi.org/10.1016/j.bios.2022.114601DOI Listing
August 2022

Recent ring distortion reactions for diversifying complex natural products.

Nat Prod Rep 2022 Aug 16. Epub 2022 Aug 16.

School of Pharmacy, Nantong University, Nantong 226001, China.

Covering: 2013-2022.Chemical diversification of natural products is an efficient way to generate natural product-like compounds for modern drug discovery programs. Utilizing ring-distortion reactions for diversifying natural products would directly alter the core ring systems of small molecules and lead to the production of structurally complex and diverse compounds for high-throughput screening. We review the ring distortion reactions recently used in complexity-to-diversity (CtD) and pseudo natural products (pseudo-NPs) strategies for diversifying complex natural products. The core ring structures of natural products are altered ring expansion, ring cleavage, ring edge-fusion, ring spiro-fusion, ring rearrangement, and ring contraction. These reactions can rapidly provide natural product-like collections with properties suitable for a wide variety of biological and medicinal applications. The challenges and limitations of current ring distortion reactions are critically assessed, and avenues for future improvements of this rapidly expanding field are discussed. We also provide a toolbox for chemists for the application of ring distortion reactions to access natural product-like molecules.
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http://dx.doi.org/10.1039/d2np00027jDOI Listing
August 2022

[Zoning and Safe Utilization Method of Heavy Metal Contaminated Cultivated Land at Block Scale].

Huan Jing Ke Xue 2022 Aug;43(8):4190-4198

Southeast Sichuan Geological Group, Chongqing Bureau of Geology and Minerals Exploration, Chongqing 400038, China.

The safe use of arable land is one of the important measures to ensure food security and to realize the construction of ecological civilization. In order to solve the problem of blindly selecting technical measures in the process of safe use and restoration of pollution-risk cultivated land, 244 surface soil samples and 100 sets of rice-root soil samples were collected in Echi Town, Qianjiang District, Chongqing. Based on the contents of five heavy metals of Cd, Hg, Pb, As, and Cr, as well as soil oxides, organic matter, and pH and other indicators, a prediction model was established using multiple regression and geostatistical analyses, and the plots were assigned values, combined with the soil and soil in the plots. The total amount of heavy metals in the rice, the available content of heavy metals in the soil, and the pH of the soil guided suggestions for the safe use of cultivated land at the corresponding plot scale. The results showed that the soil in the study area was mainly and strongly acidic. The percentages of Cd exceeding the soil pollution risk screening value and control value were 33.61% and 2.05%, respectively. The effective content of Cd accounted for 60%, and the Cd exceeding rate of rice was 28%. There was an obvious ecological risk of Cd in the study area. The available Cd content of the soil was mainly affected by the total amount of soil Cd and pH. The enrichment of Cd in rice was mainly affected by the content of soil organic matter, Mn, and CaO. The zoning results showed that the priority protection category of the soil in the study area accounted for 59.30%; the safe use category accounted for 40.44%, of which safe use (IAa), safe use (IAb), safe use (IBa), safe use (IIAa), safe use (IIAb), and safe use (IIBa) accounted for 19.49%, 8.01%, 1.43%, 7.04%, 1.41%, and 3.06%, respectively; and strict control accounted for 0.26%. This method combined the safety risks of soil and agricultural products and aided formation of specific recommendations for safe use, which provided a method of reference for the safe use of contaminated farmland in accordance with local conditions.
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http://dx.doi.org/10.13227/j.hjkx.202111163DOI Listing
August 2022

The anti-obesity effects of a water-soluble glucan from the modulation of chronic inflammation.

Front Immunol 2022 28;13:962341. Epub 2022 Jul 28.

School of Life Sciences, Jilin University, Changchun, China.

Polysaccharides from () have anti-obesity and anti-inflammatory activities. In this study, the major type, molecular weight, homogeneity and structure of a polysaccharide purified from (denoted GFPA) were determined. In high-fat diet (HFD)-treated mice, 8 weeks of GFPA administration efficiently decreased body weight and blood glucose concentration and counteracted hyperlipidemia. GFPA efficiently decreased adipocyte size and ameliorated inflammatory infiltration in the three types of white adipose tissue and alleviated steatosis, fat accumulation and inflammatory infiltration in the livers of HFD-fed mice. GFPA also decreased the concentrations of aspartate aminotransferase, alanine aminotransferase and pro-inflammatory factors in the sera and livers of HFD-treated mice. Furthermore, GFPA was found to regulate lipid metabolism the inhibition of ceramide levels in HFD-treated mice. GFPA exhibited strong anti-obesity effects the modulation of chronic inflammation through Toll-like receptor 4/nuclear factor kappa-B signaling, which supports the use of GFPA for the treatment of obesity.
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http://dx.doi.org/10.3389/fimmu.2022.962341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367694PMC
August 2022

Diagnosing Diabetic Retinopathy in OCTA Images Based on Multilevel Information Fusion Using a Deep Learning Framework.

Comput Math Methods Med 2022 4;2022:4316507. Epub 2022 Aug 4.

Faculty of Information Technology, Beijing University of Technology, Beijing 100124, China.

Objective: As an extension of optical coherence tomography (OCT), optical coherence tomographic angiography (OCTA) provides information on the blood flow status at the microlevel and is sensitive to changes in the fundus vessels. However, due to the distinct imaging mechanism of OCTA, existing models, which are primarily used for analyzing fundus images, do not work well on OCTA images. Effectively extracting and analyzing the information in OCTA images remains challenging. To this end, a deep learning framework that fuses multilevel information in OCTA images is proposed in this study. The effectiveness of the proposed model was demonstrated in the task of diabetic retinopathy (DR) classification.

Method: First, a U-Net-based segmentation model was proposed to label the boundaries of large retinal vessels and the foveal avascular zone (FAZ) in OCTA images. Then, we designed an isolated concatenated block (ICB) structure to extract and fuse information from the original OCTA images and segmentation results at different fusion levels.

Results: The experiments were conducted on 301 OCTA images. Of these images, 244 were labeled by ophthalmologists as normal images, and 57 were labeled as DR images. An accuracy of 93.1% and a mean intersection over union (mIOU) of 77.1% were achieved using the proposed large vessel and FAZ segmentation model. In the ablation experiment with 6-fold validation, the proposed deep learning framework that combines the proposed isolated and concatenated convolution process significantly improved the DR diagnosis accuracy. Moreover, inputting the merged images of the original OCTA images and segmentation results further improved the model performance. Finally, a DR diagnosis accuracy of 88.1% (95%CI ± 3.6%) and an area under the curve (AUC) of 0.92 were achieved using our proposed classification model, which significantly outperforms the state-of-the-art classification models. As a comparison, an accuracy of 83.7 (95%CI ± 1.5%) and AUC of 0.76 were obtained using EfficientNet. . The visualization results show that the FAZ and the vascular region close to the FAZ provide more information for the model than the farther surrounding area. Furthermore, this study demonstrates that a clinically sophisticated designed deep learning model is not only able to effectively assist in the diagnosis but also help to locate new indicators for certain illnesses.
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http://dx.doi.org/10.1155/2022/4316507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371870PMC
August 2022

Monocarboxylate transporter upregulation in induced regulatory T cells promotes resistance to anti-PD-1 therapy in hepatocellular carcinoma patients.

Front Oncol 2022 28;12:960066. Epub 2022 Jul 28.

Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Background: Programmed cell death-1 (PD-1) immune checkpoint inhibitors are not effective in treating all patients with hepatocellular carcinoma (HCC), and regulatory T cells (Tregs) may determine the resistance to anti-PD-1 therapy.

Methods: Patients were divided into two groups based on the clinical efficacy of anti-PD-1 therapy. Flow cytometry was used to determine the phenotype of CD4+, CD8+, and Tregs in peripheral blood mononuclear cells (PBMCs). CD4+CD45RA+T cells were sorted to analyze Treg differentiation and function.

Results: No significant differences were found between resistant and sensitive patients in the percentage of CD4+ T cells and Tregs in PBMCs or the differentiation and function of induced Tregs (iTregs). However, iTregs from resistant patients presented higher monocarboxylate transporter (MCT) expression. Lactate induced more iTregs and improved OXPHOS levels in the resistant group. MCT1 and MCT2 were highly expressed in tumor-infiltrating Tregs, and patients with higher MCT1 expression had worse clinical outcomes. Combinatorial therapy with MCT antibody and anti-PD-1 therapy effectively inhibited tumor growth.

Conclusion: MCT and its downstream lactate signal in Tregs can confer anti-PD-1 resistance and may be a marker of poor prognosis in HCC.
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http://dx.doi.org/10.3389/fonc.2022.960066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368998PMC
July 2022

Intelligence Correlates with the Temporal Variability of Brain Networks.

Neuroscience 2022 Aug 11. Epub 2022 Aug 11.

Key Laboratory of Cognition and Personality (SWU), Ministry of Education, Chongqing, China; Department of Psychology, Southwest University, Chongqing, China. Electronic address:

Intelligence is the ability to recognize and understand objective things, and use knowledge and experience to solve problems. Highly intelligent people show the ability to switch between different thought patterns and shift their mental focus. This suggests a link between intelligence and the dynamic interaction of brain networks. Thus, we investigated the relationships between resting-state dynamic brain network remodeling (temporal variability) and scores on the Wechsler Adult Intelligent Scale using a large dataset comprising 606 individuals. We found that performance intelligence was associated with greater temporal variability in the functional connectivity patterns of the dorsal attention network. High variability in these areas indicates flexible connectivity patterns, which may contribute to cognitive processes such as attention selection. In addition, performance intelligence was related to greater temporal variability in the functional connectivity patterns of the salience network. Thus, this study revealed a close relationship between performance intelligence and high variability in brain networks involved in attentional choice, spatial orientation, and cognitive control.
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http://dx.doi.org/10.1016/j.neuroscience.2022.08.001DOI Listing
August 2022

A novel fungal negative-stranded RNA virus related to mymonaviruses in Auricularia heimuer.

Arch Virol 2022 Aug 13. Epub 2022 Aug 13.

Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, 130118, Jilin, China.

Here, we report the characterization of a novel (-)ssRNA mycovirus isolated from Auricularia heimuer CCMJ1222, using a combination of RNA-seq, reverse transcription polymerase chain reaction, 5' and 3' rapid amplification of cDNA ends, and Sanger sequencing. Based on database searches, sequence alignment, and phylogenetic analysis, we designated the virus as "Auricularia heimuer negative-stranded RNA virus 1" (AhNsRV1). This virus has a monopartite RNA genome related to mymonaviruses (order Mononegavirales). The AhNsRV1 genome consists of 11,441 nucleotides and contains six open reading frames (ORFs). The largest ORF encodes a putative RNA-dependent RNA polymerase; the other ORFs encode hypothetical proteins with no conserved domains or known function. AhNsRV1 is the first (-)ssRNA virus and the third virus known to infect A. heimuer.
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http://dx.doi.org/10.1007/s00705-022-05540-5DOI Listing
August 2022

Traction of impacted and stacked maxillary anterior teeth with precise biomechanics followed by torque control using gate spring.

Am J Orthod Dentofacial Orthop 2022 Aug 9. Epub 2022 Aug 9.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China. Electronic address:

Impaction of multiple maxillary anterior teeth is a rare condition; when it occurs, it impacts the patient's chewing function and smile esthetics, and it has always been problematic for clinicians. This case report presents an 11-year-old girl with impaction of 3 adjacent maxillary anterior teeth, including labiopalatally oriented maxillary right central and lateral incisors and a mesially inclined maxillary right canine. These 3 impacted and stacked teeth were sequentially aligned after surgical exposure and orthodontic traction, and the surrounding dentigerous cyst healed simultaneously. Precise and delicate biomechanics will control root movement and prevent root collision. Gate spring, a novel torquing auxiliary, was used for highly efficient root torque movements of the previously impacted incisors. After 32-month treatment, the patient's occlusion and esthetics greatly improved.
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http://dx.doi.org/10.1016/j.ajodo.2021.04.036DOI Listing
August 2022

Knockout of Leads to Autism-like Behaviors and Developmental Delay in Zebrafish.

Int J Mol Sci 2022 Jul 29;23(15). Epub 2022 Jul 29.

Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

mutations have been associated with autism spectrum disorder (ASD) and other related neurodevelopmental disorders (NDDs) such as intellectual disability (ID) in several cohorts. has been implicated in brain development, as it is required for ciliogenesis in and is required for dendritic arborization in mice. However, a causative relationship between the disruption of Katnal2 function and behavioral defects has not been established. Here, we generated a null allele in zebrafish using CRISPR/Cas9-mediated genome editing and carried out morphological and behavioral characterizations. We observed that embryos displayed delayed embryonic development especially during the convergence and extension (CE) movement. The hatched larvae showed reduced brain size and body length. In the behavioral tests, the zebrafish exhibited reduced locomotor activity both in larvae and adults; increased nocturnal waking activity in larvae; and enhanced anxiety-like behavior, impaired social interaction, and reduced social cohesion in adults. These findings indicate an important role for in development and behavior, providing an in vivo model to study the mechanisms underlying the ASD related to mutations.
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http://dx.doi.org/10.3390/ijms23158389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368773PMC
July 2022

Inhibition Effect and Mechanism Explanation of Perilla Seed Extract as a Green Corrosion Inhibitor on Q235 Carbon Steel.

Materials (Basel) 2022 Aug 5;15(15). Epub 2022 Aug 5.

College of Water Conservancy and Hydropower, Hohai University, Nanjing 210098, China.

The development of environmentally friendly corrosion inhibitors has become a research hotspot. Aiming at the potential corrosion inhibition effect of perilla seed extract on Q235 carbon steel, the corrosion inhibition effect was quantitatively evaluated by various research methods, and the effective corrosion inhibition composition and mechanism were discussed. The research methods include potentiodynamic polarization curve method, HPLC-MS, FT-IR, XPS and chemical calculation. The experimental results show that the inhibitor prepared from perilla seed extract is a mixed inhibitor, and its adsorption behavior accords with Langmuir adsorption theory and its adsorption free energy is -22.70 kJ/mol. Combined with the experimental results and theoretical calculation, the effective corrosion inhibiting components are luteolin and apigenin. Theoretical calculation shows that both of them are adsorbed parallel to the surface of carbon steel to form thin films. The adsorption mechanism is that carbonyl O atoms in luteolin and apigenin hybridize with the 3 d empty orbit of Fe. From the point of view of quantum chemistry, the smaller the HOMO value and the energy gap value, the better the adsorption of corrosion inhibitor on the surface of carbon steel. From the point of view of molecular dynamics simulation, the greater the absolute value of adsorption energy, the better the adsorption of corrosion inhibitor on carbon steel surface.
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http://dx.doi.org/10.3390/ma15155394DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9369877PMC
August 2022

Analysis of the Elderly's Preferences for Choosing Medical Service Facilities from the Perspective of Accessibility: A Case Study of Tertiary General Hospitals in Hefei, China.

Int J Environ Res Public Health 2022 Aug 1;19(15). Epub 2022 Aug 1.

College of Architecture and Art, Hefei University of Technology, Hefei 230009, China.

The accessibility of medical service facilities is a vital influence on elderly people choosing medical treatment. Encouraging residents to seek nearby medical treatment can facilitate the rational layout and diversion of urban medical facilities and reduce health resource waste. However, due to accessibility factors, elderly people may not choose the nearest hospital. In order to investigate such factors, we conducted a questionnaire survey among the elderly from 10 tertiary general hospitals in Hefei, China. On the basis of the origin-destination (OD) cost matrix analysis and statistical analysis of 830 valid questionnaires, this paper analyzed the elders' selection rules when choosing medical facilities and the factors considered when making a choice. The study found that although 85% of elderly participants valued a short distance to tertiary hospitals, only 31% of them attended the closest hospitals in reality, which correlated with regularity according to their education level, travel activity status, and place of residence. The elderly highlighted road congestion, convenience of public transport stations, and number of transfers as critical in determining whether they sought nearby medical treatment. According to the results, effective ways to encourage the elderly to attend their nearest hospital, from the perspective of accessibility, include easing road congestion, improving the layout of public transport stations, and optimizing urban public transport routes. In particular, when planning future medical facilities, attention should be paid to the elderly with primary school education or below, who cannot travel independently, and those who live far from the city center.
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http://dx.doi.org/10.3390/ijerph19159432DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367885PMC
August 2022

Excessive Oxalic Acid Secreted by Inhibits the Growth of Mycelia during Its Saprophytic Process.

Cells 2022 Aug 5;11(15). Epub 2022 Aug 5.

College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.

is an edible and medicinal mushroom in Asia commercially cultivated on substrates containing pine sawdust. Its slow mycelial growth rate greatly increases the cultivation cycle. In this study, we mainly studied the role of oxalic acid (OA) secreted by in its saprophytic process. Our results show that crystals observed on the mycelial surface contained calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) according to X-ray diffraction (XRD). Vegetative mycelia secreted large amounts of OA during extended culture periods. However, high concentrations of OA decreased the mycelial growth rate significantly. Moreover, the degradation of lignocellulose was significantly inhibited under high concentrations of OA. These changes could be attributed to the significantly decreased activities of lignocellulose-degrading enzymes. In conclusion, by establishing a link between OA secretion by the mycelium and the slow growth rate of its saprophytic process, this work provides fundamental information for shortening the cultivation cycle of .
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http://dx.doi.org/10.3390/cells11152423DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368360PMC
August 2022

HDAC inhibitor chidamide synergizes with venetoclax to inhibit the growth of diffuse large B-cell lymphoma via down-regulation of MYC, BCL2, and TP53 expression.

J Zhejiang Univ Sci B 2022 Aug;23(8):666-681

Department of Hematology, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, China.

Diffuse large B-cell lymphoma (DLBCL) is an aggressive type of non-Hodgkin's lymphoma. A total of 10%‒15% of DLBCL cases are associated with myelocytomatosis viral oncogene homolog() and/or B-cell lymphoma-2 () translocation or amplification. BCL2 inhibitors have potent anti-tumor effects in DLBCL; however, resistance can be acquired through up-regulation of alternative anti-apoptotic proteins. The histone deacetylase (HDAC) inhibitor chidamide can induce BIM expression, leading to apoptosis of lymphoma cells with good efficacy in refractory recurrent DLBCL. In this study, the synergistic mechanism of chidamide and venetoclax in DLBCL was determined through in vitro and in vivo models. We found that combination therapy significantly reduced the protein levels of MYC, TP53, and BCL2 in activated apoptotic-related pathways in DLBCL cells by increasing BIM levels and inducing cell apoptosis. Moreover, combination therapy regulated expression of multiple transcriptomes in DLBCL cells, involving apoptosis, cell cycle, phosphorylation, and other biological processes, and significantly inhibited tumor growth in DLBCL-bearing xenograft mice. Taken together, these findings verify the in vivo therapeutic potential of chidamide and venetoclax combination therapy in DLBCL, warranting pre-clinical trials for patients with DLBCL.
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http://dx.doi.org/10.1631/jzus.B2200016DOI Listing
August 2022

Investigating distortions in perceptual stability during different self-movements using virtual reality.

Perception 2022 Aug 9:3010066221116480. Epub 2022 Aug 9.

Virtual Reality Research (VR2) Facility, Division of Neuroscience and Experimental Psychology, 12203University of Manchester, Manchester, UK.

Using immersive virtual reality (the HTC Vive Head Mounted Display), we measured both bias and sensitivity when making judgements about the scene stability of a target object during both active (self-propelled) and passive (experimenter-propelled) observer movements. This was repeated in the same group of 16 participants for three different observer-target movement conditions in which the instability of a target was yoked to the movement of the observer. We found that in all movement conditions that the target needed to move with (in the same direction) as the participant to be perceived as scene-stable. Consistent with the presence of additional available information (efference copy) about self-movement during active conditions, biases were smaller and sensitivities to instability were higher in these relative to passive conditions. However, the presence of efference copy was clearly not sufficient to completely eliminate the bias and we suggest that the presence of additional visual information about self-movement is also critical. We found some (albeit limited) evidence for correlation between appropriate metrics across different movement conditions. These results extend previous findings, providing evidence for consistency of biases across different movement types, suggestive of common processing underpinning perceptual stability judgements.
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http://dx.doi.org/10.1177/03010066221116480DOI Listing
August 2022

Molecular insights into the catalytic oxidation of methanol-to-olefins wastewater with phosphoric acid modified sludge biochar.

Chemosphere 2022 Aug 6;307(Pt 2):135938. Epub 2022 Aug 6.

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China. Electronic address:

With the development of methanol-to-olefin (MTO) process, the effective disposal of wastewater was one key factor for the long-period and benign development of this technology. Herein, a sludge-based biochar catalyst (GSC-P) was synthesized and used in photo-Fenton reaction for the degradation of MTO wastewater from the outlet of a biological aerated filter. More iron was distributed on the surface of GSC-P catalyst, facilitating the photo-Fenton oxidation of MTO wastewater, with chemical oxygen demand (COD) removal rate of 75.4% and total organic carbon (TOC) removal rate of 62.5%. The 2223 unique molecular formulas assigned by a Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) in the original MTO wastewater showed that CHO compounds shared the lowest peak numbers (20.2%) but the highest peak abundance (51.6%) among the four groups. Besides, lipids, unsaturated hydrocarbons, lignins and proteins were the main structural types. After photo-Fenton treatment of 60 min, there were 56.7%-74.0% of compounds removed by the analysis of van Krevelen diagram, indicating that the MTO wastewater was degraded efficiently. Three possible evolution processes of dissolved organic matters during the photo-Fenton reaction were disclosed at the molecular-level.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135938DOI Listing
August 2022

Identification of the 12-oxo-phytoeienoic acid reductase (OPR) gene family in pepper (Capsicum annuum L.) and functional characterization of CaOPR6 in pepper fruit development and stress response.

Genome 2022 Aug 9. Epub 2022 Aug 9.

Jiangxi Agricultural University, Nanchang, Jiangxi, China;

The 12-oxo-phytoeienoic acid reductase (OPR) is a kind of enzyme in octadecanoid biosynthesis pathway, which determines the biosynthesis of jasmonic acid. Although the roles of OPRs have been extensively studied in several crop plants, little is known about the biological functions of OPR encoding genes in Capsicum annuum plants. In this study, seven OPR family genes (CaOPR1-7) were identified from the C. annuum genome. The physical and chemical properties of CaOPR1-7 were further analyzed, including gene expression patterns, promoter elements and chromosomal locations. The results showed that the seven CaOPR homologous could be divided into two subgroups, and CaOPR6 was highly similar to AtOPR3 in Arabidopsis. The expression of CaOPR6 was significantly induced by various stresses such as cold, salt and pathogen infection, indicating that CaOPR6 plays important roles in response to abiotic and biotic stresses. Overall, these findings improve the understanding of the biological functions of CaOPR6 in the development of pepper fruit and stress response of pepper plants, and facilitate further studies on the molecular biology of OPR proteins in Solanaceae vegetables.
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http://dx.doi.org/10.1139/gen-2022-0037DOI Listing
August 2022

Effect of a Peer-Led Behavioral Intervention for Emergency Department Patients at High Risk of Fatal Opioid Overdose: A Randomized Clinical Trial.

JAMA Netw Open 2022 Aug 1;5(8):e2225582. Epub 2022 Aug 1.

Brown University School of Public Health, Providence, Rhode Island.

Importance: Fatal and nonfatal opioid overdoses are at record levels, and emergency department (ED) visits may be an opportune time to intervene. Peer-led models of care are increasingly common; however, little is known about their effectiveness.

Objective: To evaluate the effect of a peer-led behavioral intervention compared with the standard behavioral intervention delivered in the ED on engagement in substance use disorder (SUD) treatment within 30 days after the ED encounter.

Design, Setting, And Participants: This randomized clinical trial recruited 648 patients from 2 EDs from November 15, 2018, to May 31, 2021. Patients were eligible to participate if they were in the ED for an opioid overdose, receiving treatment related to an opioid use disorder, or identified as having had a recent opioid overdose.

Interventions: Participants were randomly assigned to receive a behavioral intervention from a certified peer recovery specialist (n = 323) or a standard intervention delivered by a hospital-employed licensed clinical social worker (n = 325). A certified peer recovery specialist was someone with at least 2 years of recovery who completed a 45-hour training program and had 500 hours of supervised work experience. After the ED intervention, the certified peer recovery specialists offered continued contact with participants for up to 90 days.

Main Outcomes And Measures: The primary outcome was receipt of SUD treatment within 30 days of enrollment, assessed with deterministic linkage of statewide administrative databases. Treatment engagement was defined as admission to a formal, publicly licensed SUD treatment program or receipt of office-based medication for opioid use disorder within 30 days of the initial ED visit.

Results: Among the 648 participants, the mean (SD) age was 36.9 (10.8) years, and most were male (442 [68.2%]) and White (444 [68.5%]). Receipt of SUD treatment occurred for 103 of 323 participants (32%) in the intervention group vs 98 of 325 participants (30%) in the usual care group within 30 days of the ED visit. Among all participants, the most accessed treatments were outpatient medication for opioid use disorder (buprenorphine, 119 [18.4%]; methadone, 44 [6.8%]) and residential treatment (44 [6.8%]).

Conclusions And Relevance: Overall, this study found that a substantial proportion of participants in both groups engaged in SUD treatment within 30 days of the ED visit. An ED-based behavioral intervention is likely effective in promoting treatment engagement, but who delivers the intervention may be less influential on short-term outcomes. Further study is required to determine the effects on longer-term engagement in SUD care and other health outcomes (eg, recurrent overdose).

Trial Registration: ClinicalTrials.gov Identifier: NCT03684681.
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http://dx.doi.org/10.1001/jamanetworkopen.2022.25582DOI Listing
August 2022

Progress on aptamer-based SERS sensors for food safety and quality assessment: methodology, current applications and future trends.

Crit Rev Food Sci Nutr 2022 Aug 9:1-18. Epub 2022 Aug 9.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, People's Republic of China.

It is well known that food safety has aroused extensive attentions from governments to researchers and to food industries. As a versatile technology based on molecular interactions, aptamer sensors which could specifically identify a wide range of food contaminants have been extensively studied in recent years. Surface-enhanced Raman spectroscopy integrated aptamer combines the advantages of both technologies, not only in the ability to specifically identify a wide range of food contaminants, but also in the ultra-high sensitivity, simplicity, portable and speed. To provide beneficial insights into the evaluation techniques in the field of food safety, we offer a comprehensive review on the design strategies for aptamer-SERS sensors in different scenarios, including non-nucleic acid amplification methods ("on/off" mode, sandwich mode, competition model and catalytic model) and nucleic acid amplification methods (hybridization chain reaction, rolling circle amplification, catalytic hairpin assembly). Meanwhile, a special attention is paid to the application of aptamer-SERS sensors in biological (foodborne pathogenic, bacteria and mycotoxins) and chemical contamination (drug residues, metal ions, and food additives) of food matrix. Finally, the challenges and prospects of developing reliable aptamer-SERS sensors for food safety were discussed, which are expected to offer a strong guidance for further development and extended applications.
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http://dx.doi.org/10.1080/10408398.2022.2108370DOI Listing
August 2022

Host Plant Selection Imprints Structure and Assembly of Fungal Community along the Soil-Root Continuum.

mSystems 2022 Aug 9:e0036122. Epub 2022 Aug 9.

College of Biology and the Environment, Nanjing Forestry University, Nanjing, China.

The soil fungal community plays pivotal roles in soil nutrient cycling and plant health and productivity in agricultural ecosystems. However, the differential adaptability of soil fungi to different microenvironments (niches) is a bottleneck limiting their application in agriculture. Hence, the understanding of ecological processes that drive fungal microbiome assembly along the soil-root continuum is fundamental to harnessing the plant-associated microbiome for sustainable agriculture. Here, we investigated the factors that shape fungal community structure and assembly in three compartment niches (the bulk soil, rhizosphere, and rhizoplane) associated with tobacco (Nicotiana tabacum L.), with four soil types tested under controlled greenhouse conditions. Our results demonstrate that fungal community assembly along the soil-root continuum is governed by host plant rather than soil type and that soil chemical properties exert a negligible effect on the fungal community assembly in the rhizoplane. Fungal diversity and network complexity decreased in the order bulk soil > rhizosphere > rhizoplane, with a dramatic decrease in Ascomycota species number and abundance along the soil-root continuum. However, facilitations (positive interactions) were enhanced among fungal taxa in the rhizoplane niche. The rhizoplane supported species specialization with enrichment of some rare species, contributing to assimilative community assembly in the rhizoplane in all soil types. and were identified as important indicator genera of the soil-root microbiome continuum and good predictors of plant agronomic traits. The findings provide empirical evidence for host plant selection and enrichment/depletion processes of fungal microbiome assembly along the soil-root continuum. Fungal community assembly along the soil-root continuum is shaped largely by the host plant rather than the soil type. This finding facilitates the implementations of fungi-associated biocontrol and growth-promoting for specific plants in agriculture practice, regardless of the impacts from variations in geographical environments. Furthermore, the depletion of complex ecological associations in the fungal community along the soil-root continuum and the enhancement of facilitations among rhizoplane-associated fungal taxa provide empirical evidence for the potential of community simplification as an approach to target the plant rhizoplane for specific applications. The identified indicators and along the soil-root microbiome continuum are good predictors of tobacco plant agronomic traits, which should be given attention when manipulating the root-associated microbiome.
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http://dx.doi.org/10.1128/msystems.00361-22DOI Listing
August 2022

Set2 family regulates mycotoxin metabolism and virulence via H3K36 methylation in pathogenic fungus .

Virulence 2022 Dec;13(1):1358-1378

Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.

infects various crops with aflatoxins, and leads to aspergillosis opportunistically. Though H3K36 methylation plays an important role in fungal toxin metabolism and virulence, no data about the biological function of H3K36 methylation in virulence has been reported. Our study showed that the Set2 histone methyltransferase family, AshA and SetB, involves in morphogenesis and mycotoxin anabolism by regulating related transcriptional factors, and they are important for fungal virulence to crops and animals. Western-blotting and double deletion analysis revealed that AshA mainly regulates H3K36me2, whereas SetB is mainly responsible for H3K36me3 in the nucleus. By construction of domain deletion strain and point mutation strains by homologous recombination, the study revealed that SET domain is indispensable in mycotoxin anabolism and virulence of , and N455 and V457 in it are the key amino acid residues. ChIP analysis inferred that the methyltransferase family controls fungal reproduction and regulates the production of AFB1 by directly regulating the production of the transcriptional factor genes, including , , and amylase, through H3K36 trimethylation in their chromatin fragments, based on which this study proposed that, by H3K36 trimethylation, this methyltransferase family controls AFB1 anabolism through transcriptional level and substrate utilization level. This study illuminates the epigenetic mechanism of the Set2 family in regulating fungal virulence and mycotoxin production, and provides new targets for controlling the virulence of the fungus .The methylation of H3K36 plays an important role in the fungal secondary metabolism and virulence, but no data about the regulatory mechanism of H3K36 methylation in the virulence of have been reported. Our study revealed that, in the histone methyltransferase Set2 family, AshA mainly catalyzes H3K36me2, and involves in the methylation of H3K36me1, and SetB mainly catalyzes H3K36me3 and H3K36me1. Through domain deletion and point mutation analysis, this study also revealed that the SET domain was critical for the normal biological function of the Set2 family and that N455 and V457 in the domain were critical for AshA. By ChIP-seq and ChIP-qPCR analysis, H3K36 was found to be trimethylation modified in the promotors and ORF positions of , and the amylase gene (AFLA_084340), and further qRT-PCR results showed that these methylation modifications regulate the expression levels of these genes. According to the results of ChIP-seq analysis, we proposed that, by H3K36 trimethylation, this methyltransferase family controls the metabolism of mycotoxin through transcriptional level and substrate utilization level. All the results from this study showed that Set2 family is essential for fungal secondary metabolism and virulence, which lays a theoretical groundwork in the early prevention and treatment of pollution, and also provides an effective strategy to fight against other pathogenic fungi.
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http://dx.doi.org/10.1080/21505594.2022.2101218DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9364737PMC
December 2022

Growth, biofilm formation and atrazine degrading gene (trzN) expression of Arthrobacter sp. DNS10 cultured with montmorillonite, kaolinite and goethite.

Chemosphere 2022 Aug 5;307(Pt 2):135904. Epub 2022 Aug 5.

School of Resources &; Environment, Northeast Agricultural University, Harbin, 150030, PR China; Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130132, PR China. Electronic address:

The viable and degradation potential of the strains which adhered to soil minerals are essential for eliminating organic pollutants from soil. Herein, the interaction (growth, biofilm formation and survive) of Arthrobacter sp. DNS10, an atrazine degrading strain, with three kinds of typical soil minerals, such as montmorillonite, kaolinite and goethite, as well as the atrazine degradation gene (trzN) expression of the strain in the minerals system were studied. The results showed that montmorillonite had significant promotion effect on the growth of strain DNS10, followed by kaolinite, but goethite significantly inhibited the growth of strain DNS10. In contrast, goethite notably promoted the biofilm formation and there was less biofilm detected in montmorillonite containing system. The percentage of the survival bacteria in the biofilm that formed on montmorillonite, kaolinite and goethite was 53.8%, 40.8% and 28.2%. In addition, there were more reactive oxygen species (ROS) were detected in the cells that exposed to goethite than those of the cells exposed to kaolinite and montmorillonite. These results suggest that the electrostatic repulsion between kaolinite/montmorillonite and strain DNS10 prevents them from contacting each other and facilitates bacterial growth by allowing the strain to obtain more nutrients. Oppositely, the needle-like morphology of goethite might damage the strain DNS10 cell when they were combined by electrostatic attraction, and the goethite induced ROS also aggravate the cytotoxicity of goethite on strain DNS10. In addition, the relative transcription of trzN in the cells contacted with montmorillonite, kaolinite and goethite was 0.94-, 0.27- and 0.20- fold of the no mineral exposure treatment. Briefly, this research suggests that the minerals with different structure and/or physicochemical characteristics might cause various trend for the biofilm formation and degradation potential of the bacteria.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135904DOI Listing
August 2022

Single-cell transcriptomics reveals male germ cells and Sertoli cells developmental patterns in dairy goats.

Front Cell Dev Biol 2022 22;10:944325. Epub 2022 Jul 22.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.

Spermatogenesis holds considerable promise for human-assisted reproduction and livestock breeding based on stem cells. It occurs in seminiferous tubules within the testis, which mainly comprise male germ cells and Sertoli cells. While the developmental progression of male germ cells and Sertoli cells has been widely reported in mice, much less is known in other large animal species, including dairy goats. In this study, we present the data of single cell RNA sequencing (scRNA-seq) for 25,373 cells from 45 (pre-puberty), 90 (puberty), and 180-day-old (post-puberty) dairy goat testes. We aimed to identify genes that are associated with key developmental events in male germ cells and Sertoli cells. We examined the development of spermatogenic cells and seminiferous tubules from 15, 30, 45, 60, 75, 90, 180, and 240-day-old buck goat testes. scRNA-seq clustering analysis of testicular cells from pre-puberty, puberty, and post-puberty goat testes revealed several cell types, including cell populations with characteristics of spermatogonia, early spermatocytes, spermatocytes, spermatids, Sertoli cells, Leydig cells, macrophages, and endothelial cells. We mapped the timeline for male germ cells development from spermatogonia to spermatids and identified gene signatures that define spermatogenic cell populations, such as AMH, SOHLH1, INHA, and ACTA2. Importantly, using immunofluorescence staining for different marker proteins (UCHL1, C-KIT, VASA, SOX9, AMH, and PCNA), we explored the proliferative activity and development of male germ cells and Sertoli cells. Moreover, we identified the expression patterns of potential key genes associated with the niche-related key pathways in male germ cells of dairy goats, including testosterone, retinoic acid, PDGF, FGF, and WNT pathways. In summary, our study systematically investigated the elaborate male germ cells and Sertoli cells developmental patterns in dairy goats that have so far remained largely unknown. This information represents a valuable resource for the establishment of goat male reproductive stem cells lines, induction of germ cell differentiation , and the exploration of sequential cell fate transition for spermatogenesis and testicular development at single-cell resolution.
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http://dx.doi.org/10.3389/fcell.2022.944325DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355508PMC
July 2022

Exploring Different Effects of Exclusive Enteral Nutrition (EEN) and Corticosteroids on the Gut Microbiome in Crohn's Disease Based on a Three-Stage Strategy.

Gastroenterol Res Pract 2022 27;2022:6147124. Epub 2022 Jul 27.

Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu Province, China.

The objective of this study was to compare the efficacy of exclusive enteral nutrition (EEN) and corticosteroids on the gut microbiome in Crohn's disease. . Data were collected for 16 patients newly diagnosed with CD as the test group and 10 healthy volunteers as the control group. The 16 patients were randomly divided into the EEN group and the corticosteroids group. For subsequent analysis, 6 patients in the EEN group with follow-up were enrolled to compare the 0-month, 1-month, and 3-month outcomes. We analyzed and compared gut microbiota between different groups in 3 stages. To evaluate the clinical outcome of treatment, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), hemoglobin (HB), albumin (ALB), and Crohn's disease activity (CDAI) were recorded. . There are significant differences in microbiota between patients with CD and healthy people, and there are intuitive differences in the main components of the microbiota. 16 patients were included in stage 2, in which both corticosteroids and EEN can induce CD remission well. However, corticosteroids have a greater impact on inflammatory indicators, while EEN has a more obvious effect on nutritional indicators. Principal component analysis suggests that there are different compositional changes in the gut microbiome after corticosteroids and EEN treatment. After 3 months of dynamic observation, we found that EEN can effectively maintain CD remission, reduce inflammatory indicators, and improve nutritional indicators. . Both EEN and corticosteroids can increase the diversity of the microbiome in inducing CD remission, while they have different effects on the proportion of microbiome species. This trial is registered with NCT02056418.
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http://dx.doi.org/10.1155/2022/6147124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348958PMC
July 2022

A joint constrained CCA model for network-dependent brain subregion parcellation.

IEEE J Biomed Health Inform 2022 Aug 5;PP. Epub 2022 Aug 5.

Connectivity-based brain region parcellation from functional magnetic resonance imaging (fMRI) data is complicated by heterogeneity among aged and diseased subjects, particularly when the data are spatially transformed to a common space. Here, we propose a group-guided functional brain region parcellation model capable of obtaining subregions from a target region with consistent connectivity profiles across multiple subjects, even when the fMRI signals are kept in their native spaces. The model is based on a joint constrained canonical correlation analysis (JC-CCA) method that achieves group-guided parcellation while allowing the data dimension of the parcellated regions for each subject to vary. We performed extensive experiments on synthetic and real data to demonstrate the superiority of the proposed model compared to other classical methods. When applied to fMRI data of subjects with and without Parkinson's disease (PD) to estimate the subregions in the Putamen, significant between-group differences were found in the derived subregions and the connectivity patterns. Superior classification and regression results were obtained, demonstrating its potential in clinical practice.
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http://dx.doi.org/10.1109/JBHI.2022.3196689DOI Listing
August 2022

Adipose mesenchymal stem cell sheets-derived extracellular vesicles-microRNA-10b promote skin wound healing by elevating expression of CDK6.

Biomater Adv 2022 May 1;136:212781. Epub 2022 Apr 1.

Shenzhen Beike Biotechnology Research Institute, Shenzhen 518057, PR China; Intervention and Cell Therapy Center, Shenzhen Hospital of Peking University, Shenzhen 518057, PR China. Electronic address:

Application of adipose-derived mesenchymal stromal cells (AMSCs)-derived extracellular vesicles (EVs) in skin wound healing has been documented. In this study, we investigated the therapeutic potential of AMSCs-derived EVs in skin wound healing through delivery of microRNA-10b (miR-10b). HaCaT cells were treated with HO to establish the skin wound cell models. Next, the binding affinity between miR-194, PEA15, and CDK6 was identified. Additionally, EVs were isolated from the culture medium of AMSC sheets, followed by incubation with HO-treated HaCaT cells to detect cell proliferation, migration, and apoptosis using gain- or loss-of-function experiments. Lastly, the mice skin wound models were also established to assess skin wound healing ability. miR-10b was down-regulated in the skin trauma models and enriched in the EVs of AMSC sheets. Moreover, miR-10b derived from EVs targeted PEA15 to promote CDK6 expression, thereby stimulating the proliferation and migration of HO-damaged HaCaT cells but inhibiting apoptosis. In vivo experiments further ascertained the therapeutic functionality of AMSC sheets-derived EVs-miR-10b. In summary, AMSC sheets-derived EVs carrying miR-10b promoted CDK6 expression to intensify skin wound healing by regulating PEA15.
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http://dx.doi.org/10.1016/j.bioadv.2022.212781DOI Listing
May 2022

Eudicot primary cell wall glucomannan is related in synthesis, structure and function to xyloglucan.

Plant Cell 2022 Aug 4. Epub 2022 Aug 4.

Department of Biochemistry, University of Cambridge, Hopkins Building, The Downing Site, Tennis Court Road, Cambridge CB2 1QW, UK.

Hemicelluose polysaccharides influence assembly and properties of the plant primary cell wall (PCW), perhaps by interacting with cellulose to affect the deposition and bundling of cellulose fibrils. However, the functional differences between plant cell wall hemicelluloses such as glucomannan, xylan and xyloglucan (XyG) remain unclear. As the most abundant hemicellulose, XyG is considered important in eudicot PCWs, but plants devoid of XyG show relatively mild phenotypes. We report here that a patterned β-galactoglucomannan (β-GGM) is widespread in eudicot PCWs and shows remarkable similarities to XyG. The sugar linkages forming the backbone and side chains of β-GGM are analogous to those that make up XyG, and moreover, these linkages are formed by glycosyltransferases from the same CAZy families. Solid-state nuclear magnetic resonance indicated that β-GGM shows low mobility in the cell wall, consistent with interaction with cellulose. Although Arabidopsis β-GGM synthesis mutants show no obvious growth defects, genetic crosses between β-GGM and XyG mutants produce exacerbated phenotypes compared to XyG mutants. These findings demonstrate a related role of these two similar but distinct classes of hemicelluloses in PCWs. This work opens avenues to study the roles of β-GGM and XyG in PCWs.
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http://dx.doi.org/10.1093/plcell/koac238DOI Listing
August 2022

Case Report: Prenatal Diagnosis of Nemaline Myopathy.

Front Pediatr 2022 19;10:937668. Epub 2022 Jul 19.

Department of Ultrasound Diagnostic, The Second Xiangya Hospital, Central South University, Changsha, China.

Nemaline myopathy (NM) is a rare, hereditary heterogeneous myopathy. Fetal NM has a more severe disease course and a poorer prognosis and is usually lethal during the first few months of life. Hence, early prenatal diagnosis is especially important for clinical interventions and patient counseling. We report the case of a fetus with NM due to gene variation leading to arthrogryposis multiplex congenita (AMC). The ultrasonography and histopathology results revealed an enhanced echo intensity and decreased muscle thickness, which may be novel features providing early clues for the prenatal diagnosis of NM. Moreover, to our knowledge, this article is the first report to describe a case of NM associated with complex congenital heart disease (CHD).
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http://dx.doi.org/10.3389/fped.2022.937668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343628PMC
July 2022
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