Publications by authors named "Li Yang"

12,980 Publications

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Bacillus arachidis sp. nov., Isolated from Peanut Rhizosphere Soil.

Curr Microbiol 2022 Jun 29;79(8):231. Epub 2022 Jun 29.

Center for Bioengineering and Biotechnology, College of Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, China.

A novel facultatively aerobic bacterium designated SY8 was isolated from a peanut rhizosphere soil sample collected in Jiangsu Province, China. Cells are Gram-stain-positive, rod-shaped, and agar colonies are creamy, opaque, and usually rhizoidal. Strain growth occurs at 30 - 45 °C (optimum 30 °C), pH 4.0 - 10.0 (optimum pH 6.0) and 0 - 4% (w/v) NaCl (optimum 2%) in Luria-Bertani medium. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain SY8 forms a distinct lineage in the clade of genus Bacillus and is related to Bacillus pseudomycoides DSM 12442 (99.9%). Phylogenetic analysis of the concatenated gene sequences of 16S rRNA, gryB and rpoD also indicated that strain SY8 forms a distinct lineage in Bacillus. Calculation of the average nucleotide identities and the digital DNA-DNA hybridization values between strain SY8 and the related type Bacillus strains further revealed that strain SY8 represents a distinct species. The predominant cellular fatty acids are iso C (28.7%) and summed feature 3 (Cω7c and/or Cω6c) (10.3%). The major polar lipids consisted of diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl ethanolamine, phosphatidylinositol, and three unidentified phospholipids. The major menaquinone of SY8 was MK-7. Based on phenotypic, phylogenetic, chemotaxonomic, and genomic features, strain SY8 represents a novel species of the genus Bacillus. The name Bacillus arachidis sp. nov. is proposed with strain SY8 (= CCTCC AB 2021100=LMG 32409) designated as the type strain.
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http://dx.doi.org/10.1007/s00284-022-02925-2DOI Listing
June 2022

Multiplex Accelerated PCR System for One-Step Helicobacter pylori cagA + Genotypes Detection: A Guide for Clinical Testing.

Curr Microbiol 2022 Jun 29;79(8):235. Epub 2022 Jun 29.

Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences, Department of Pathogenic Biology, School of Basic Medicine, and Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, Shandong, China.

Helicobacter pylori cagA + genotype is a leading risk factor for gastric cancer development making accurate identification and timely eradication of H. pylori critical to deadly gastric cancer prevention. Traditional clinical diagnostic methods, including conventional in vitro culture, histological examination, and (13/14)C-urea breath test methods, could only identify the presence of H. pylori, but these means are not capable of identification of cagA + strains. Herein, we firstly built a multiplex detection system based on novel accelerated PCR that could realize one-step detection of as low as 20 copies of H. pylori 16S rDNA and cagA genes within 30 min. In addition, this novel system performed strong anti-jamming capacity, and exhibited that it could specifically differentiate H. pylori cagA- and cagA + genotypes co-existence with other 4 kinds of gastrointestinal pathogens. Furthermore, this one-step system showed remarkable performance on rapid H. pylori infection diagnosis and cagA + genotypes identification in clinical gastric mucosa samples. Specifically, it outperformed histological examination in terms of accuracy and was superior to conventional PCR and DNA sequencing in terms of efficiency. This rapid, sensitive, and reliable H. pylori detection and identification system would break the limitation of traditional methods and realize H. pylori infection diagnosis and cagA + genotypes identification.
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http://dx.doi.org/10.1007/s00284-022-02931-4DOI Listing
June 2022

Elevated levels of body mass index and waist circumference, but not high variability, are associated with an increased risk of atrial fibrillation.

BMC Med 2022 Jun 29;20(1):215. Epub 2022 Jun 29.

Department of Cardiology, The First Medical Center, Chinese People's Liberation Army Hospital, Medical School of Chinese People's Liberation Army, Beijing, China.

Background: Although obesity has been associated with risk of atrial fibrillation (AF), the associations of variability of obesity measures with AF risk are uncertain, and longitudinal studies among Chinese population are still lacking. We aimed to evaluate the impacts of obesity and variability of body mass index (BMI) and waist circumference (WC) on the risk of atrial fibrillation (AF) in a large Chinese cohort study.

Methods: A total of 44,135 participants of the Kailuan Study who were free of cancer and cardiovascular disease and underwent three consecutive surveys from 2006 to 2010 were followed for incident AF until 2020. Average BMI and WC over time and variability were calculated. Cox proportional hazards regression models were used to assess hazard ratios (HRs) and 95% confidence intervals (CIs) for the associations of obesity and variability in BMI and WC with AF risk.

Results: During a mean follow-up of 9.68 years, there were 410 cases of incident AF. In multivariable-adjusted models, compared with normal BMI/WC, individuals with general obesity and abdominal obesity had increased risk of AF, with corresponding HRs of 1.73 (95% CI: 1.31-2.30) and 1.38 (95% CI: 1.11-1.60), respectively. The short-term elevation in AF risk persisted for the obese even after adjustment for updated biologic intermediaries and weight. Variability in BMI and WC were not associated with the risk of AF. The restricted cubic spline models indicated significant linear relationships between levels of WC and BMI and risk of AF.

Conclusions: Elevated levels of BMI and WC were associated with an increased risk of AF, whereas variability in BMI and WC were not. Therefore, achieving optimal levels of BMI and WC could be valuable in AF prevention.
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http://dx.doi.org/10.1186/s12916-022-02413-1DOI Listing
June 2022

Antibiotic resistant bacteria inactivation through metal-free electrochemical disinfection with carbon catalysts and its potential risks.

Chemosphere 2022 Jun 25:135496. Epub 2022 Jun 25.

School of Environment Engineering, Wuxi University, Wuxi, 214105, China.

Recently, increasing attention has been paid to the inactivation of antibiotic resistant bacteria (ARB) during the electrochemical disinfection. However, no available information could be found on ARB inactivation in water during metal-free electrochemical disinfection. In this study, polyvinylidene fluoride (PVDF)-based carbon catalyst (PPC) was chosen as working electrode. Batch experiments were conducted to investigate key design for ARB inactivation, effects of water matrix and potential risks after the disinfection under the pre-determined conditions. The disinfection with current density at 2.25 mA/cm and Air/Water ratio of 10:1 was optimal with the largest ARB inactivation (5.0 log reduction for 40 min), which was in line with the profile and yield of hydrogen peroxide (HO) during the disinfection. Effects of water matrix analysis implied that ARB inactivation efficiencies during the disinfection in acidic solutions were better than the one in alkaline solutions, which could be due to rich CC levels on surface of PPC cathode. After the optimal disinfection, ARB counts increased slightly at the first 2 h and then tended to disappear, and there were no conjugation transfer and little transformation for target antibiotic resistance genes, indicating that potential risks could be blocked after the disinfection for 40 min. Furthermore, intermittent flow was more effective in inactivating ARB compared with continuous flow. These suggested that the application of metal-free electrochemical disinfection with PPC to inactivate ARB in water was feasible and desirable in this study.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135496DOI Listing
June 2022

Normalization of tumor vasculature: A potential strategy to increase the efficiency of immune checkpoint blockades in cancers.

Int Immunopharmacol 2022 Jun 25;110:108968. Epub 2022 Jun 25.

Department of General Surgery, Shengjing Hospital of China Medical University, No.36.Sanhao stress, Shenyang 110004, Liaoning Province, China. Electronic address:

Immune checkpoint inhibitors (ICIs) eliminate tumor cells by reactivating CD8 + T cells using the cytotoxic effects of the immune system. However, in this process, tumor angiogenic factors and abnormal formation of tumor blood vessels are not conducive to the treatment of ICIs. In the tumor microenvironment (TME), proangiogenic factors prevent dendritic cell maturation, reduce T cell infiltration, and recruit inhibitory immune cells such as regulatory T (Treg) cells. Abnormal tumor blood vessels also prevent immune cells and chemotherapy drugs from reaching the target effectively and lead to poor perfusion and severe hypoxia of the tumor. Treatment with antiangiogenic inhibitors can block the transmission of abnormal angiogenesis signals and promote the normalization of tumor vasculature. Therefore, the combination of antiangiogenic inhibitors and ICIs is used in clinical therapy. Combination therapy has been proven theoretically feasible in preclinical trials, and many clinical trials have been completed to confirm its safety and efficacy.
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http://dx.doi.org/10.1016/j.intimp.2022.108968DOI Listing
June 2022

Establishment and application of a quadruple real-time RT-PCR for detecting avian metapneumovirus.

PLoS One 2022 28;17(6):e0270708. Epub 2022 Jun 28.

China Animal Health and Epidemiology Center, Qingdao, Shandong, China.

In order to develop an appropriate method for high-throughput detection of avian metapneumovirus, a quadruple real-time reverse-transcription polymerase chain reaction assay was established with four pairs of specific primers and four specific probes based on the G or M gene of aMPV-A, aMPV-B, aMPV-C and aMPV-D. Its specificity and sensitivity were evaluated, and clinical samples were tested by the method. The results showed that all the four subgroups of avian metapneumovirus can be detected in the quadruple real-time RT-PCR assay simultaneously, with a detection limit of 100-1000 cRNA copies/reaction. The other common poultry viruses were negative. In the avian clinical sample detection, 39 out of 1920 clinical samples collected from 8 provinces were positive. Compared with published RT-PCR assays, the κ value of the quadruple real-time RT-PCR assay in 1920 avian clinical samples was 1.000 (P < 0.001). The established method could be used for the rapid detection of the four subgroups of avian metapneumovirus with high specificity and high sensitivity.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0270708PLOS
June 2022

Lipid Droplet-Specific Dual-Response Fluorescent Probe for the Detection of Polarity and HO and Its Application in Living Cells.

Anal Chem 2022 Jun 28. Epub 2022 Jun 28.

School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, P. R. China.

HO and polarity are quite important in many physiological and pathological processes, and their relationship is complicated and obscure for researchers. Thus, it is vital and challenging to achieve simultaneous detection of HO and polarity . Herein, the first naphthalimide-triphenylamine-based dual-site fluorescent probe is developed for simultaneously imaging intracellular HO and polarity fluctuations. It exhibits excellent sensitivity (LOD = 44 nM), selectivity, and fast response (15 min) to HO and a superior capacity for detecting polarity upon the intramolecular charge transfer (ICT) effect. Besides, the probe displays low cytotoxicity and lipid droplet targeting and is further applied in imaging HO and polarity fluctuations in HepG2 and L-02 cells, so that is qualified to distinguish cancer cells from normal cells. This research contributes a new design principle for the construction of dual-site fluorescent probes for simultaneously detecting active molecules and polarity.
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http://dx.doi.org/10.1021/acs.analchem.2c01243DOI Listing
June 2022

Two Pre-Expanded Free Flaps Used for Auricular Reconstruction and Ipsilateral Facial Scar Release.

J Craniofac Surg 2022 Jun 28. Epub 2022 Jun 28.

Department of Plastic and Reconstructive Surgery, Xijing Hospital of Forth Military Medical University, Xi'an, China.

Objectives: Auricular reconstruction is a great challenge for surgeons to achieve good aesthetic outcomes when adjacent tissues were burned. Compared with pedicle flap therapies, there are some advantages of pre-expanded free flaps for ear rebuilding, such as thinner layer tissues for aesthetic requirements of delicate auricular structures and less donor site deformity. In this study, the authors introduced 6 sequential surgical procedures for total auricular reconstruction with severe ipsilateral facial scar.

Methods: Pre-expanded deltopectoral flap was used to release periauricular contracture and repair facial scar. The injured ear was restored by expanded forearm flap including autologous cartilage framework. The surgical procedures were lasted more than 2 years. An 8 and half year's follow-up was performed from November 2012 to April 2021. The clinical data and surgical techniques were recorded and analyzed.

Results: The patient was satisfied with the aesthetic outcomes of the new ear. The skin texture and color of the grafts were approximately matched to the recipient sites. Facial expression was not affected severely. Sensations of the transferred flap and new ear had partially recovered. The donor sites were recovered without severe complication.

Conclusions: The pre-expanded free forearm flap is a feasible method for total ear reconstruction when local flap therapies could not be applied. Repair of ipsilateral facial scar is beneficial for auricular procedures.
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http://dx.doi.org/10.1097/SCS.0000000000008669DOI Listing
June 2022

[Preadmission follow-up condition of neonates hospitalized due to severe hyperbilirubinemia after discharge from the department of obstetrics and influencing factors for follow-up compliance: a multicenter investigation].

Zhongguo Dang Dai Er Ke Za Zhi 2022 Jun;24(6):669-674

Department of Pediatrics, Women's Hospital of Nanjing Medical University, Nanjing 210004, China.

Objectives: To investigate the preadmission follow-up condition of neonates hospitalized due to severe hyperbilirubinemia after discharge from the department of obstetrics and the influencing factors for follow-up compliance.

Methods: A multicenter retrospective case-control study was performed for the cases from the multicenter clinical database of 12 units in the Quality Improvement Clinical Research Cooperative Group of Neonatal Severe Hyperbilirubinemia in Jiangsu Province of China from January 2019 to April 2021. According to whether the follow-up of neonatal jaundice was conducted on time after discharge from the department of obstetrics, the neonates were divided into two groups: good follow-up compliance and poor follow-up compliance. The multivariate logistic regression model was used to identify the influencing factors for follow-up compliance of the neonates before admission.

Results: A total of 545 neonates with severe hyperbilirubinemia were included in the study, with 156 neonates (28.6%) in the good follow-up compliance group and 389 (71.4%) in the poor follow-up compliance group. The multivariate logistic regression analysis showed that low gestational age at birth, ≥10% reduction in body weight on admission compared with birth weight, history of phototherapy of siblings, history of exchange transfusion of siblings, Rh(-) blood type of the mother, a higher educational level of the mother, the use of WeChat official account by medical staff to remind of follow-up before discharge from the department of obstetrics, and the method of telephone notification to remind of follow-up after discharge were associated with the increase in follow-up compliance (<0.05).

Conclusions: Poor follow-up compliance is observed for the neonates with severe hyperbilirubinemia after discharge from the department of obstetrics, which suggests that it is necessary to further strengthen the education of jaundice to parents before discharge and improve the awareness of jaundice follow-up. It is recommended to remind parents to follow up on time by phone or WeChat official account.
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http://dx.doi.org/10.7499/j.issn.1008-8830.2112113DOI Listing
June 2022

Poor outcome of pediatric patients with acute myeloid leukemia harboring high FLT3/ITD allelic ratios.

Nat Commun 2022 Jun 27;13(1):3679. Epub 2022 Jun 27.

Children's Medical Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, 510120, PR China.

Activating FLT3 mutations are the most common mutations in acute myeloid leukemia (AML), but the optimal threshold of FLT3/ITD allelic ratio (AR) among pediatric AML patients remains controversial. Here, we present the outcome and prognostic significance of FLT3/ITD AR analysis among pediatric patients with AML from the TARGET dataset. Applying fitting curve models and threshold effect analysis using the restrictive cubic spline function following Cox proportional hazards models identifies the cut-off value of 0.5 on FLT3/ITD AR. Moreover, we observe that high FLT3/ITD AR patients have an inferior outcome when compared to low AR patients. Our study also demonstrates that stem cell transplantation may improve the outcome in pediatric AML patients with high FLT3/ITD AR and may be further improved when combined with additional therapies such as Gemtuzumab Ozogamicin. These findings underline the importance of individualized treatment of pediatric AML.
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http://dx.doi.org/10.1038/s41467-022-31489-9DOI Listing
June 2022

Mice with Trp53 and Rb1 deficiency in chondrocytes spontaneously develop chondrosarcoma via overactivation of YAP signaling.

Cell Death Dis 2022 Jun 27;13(6):570. Epub 2022 Jun 27.

Department of Basic & Translational Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

Chondrosarcoma (CHS) is a rare type of soft sarcoma with increased production of cartilage matrix arising from soft bone tissues. Currently, surgical resection is the primary clinical treatment for chondrosarcoma due to the poor response to radiotherapy and chemotherapy. However, the therapeutic effect is not satisfactory due to the higher local recurrence rate. Thus, management and elucidation of the pathological mechanism of chondrosarcoma remain an ongoing challenge, and the development of effective chondrosarcoma mouse models and treatment options are urgently needed. Here, we generated a new transgenic chondrosarcoma model by double conditional deletions of Trp53 and Rb1 in chondrocyte lineage which spontaneously caused spinal chondrosarcoma and lung metastasis. Bioinformatic analysis of the human soft sarcoma database showed that Trp53 and Rb1 genes had higher mutations, reaching up to approximately 33.5% and 8.7%, respectively. Additionally, Trp53 and Rb1 signatures were decreased in the human and mouse chondrosarcoma tissues. Mechanistically, we found that YAP expression and activity were significantly increased in mouse Col2-Cre;Trp53/Rb1 chondrosarcoma tissues compared to the adjacent normal cartilage. Knockdown of YAP in primary chondrosarcoma cells significantly inhibited chondrosarcoma proliferation, invasion, and tumorsphere formation. Chondrocyte lineage ablation of YAP delayed chondrosarcoma progression and lung metastasis in Col2-Cre;Trp53/Rb1 mice. Moreover, we found that metformin served as a YAP inhibitor, which bound to the activity area of YAP protein, and inhibited chondrosarcoma cell proliferation, migration, invasion, and progression in vitro and significantly suppressed chondrosarcoma formation in vivo. Collectively, this study identifies the inhibition of YAP may be an effective therapeutic strategy for the treatment of chondrosarcoma.
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http://dx.doi.org/10.1038/s41419-022-04916-4DOI Listing
June 2022

Mild Amide Synthesis Using Nitrobenzene under Neutral Conditions.

Org Lett 2022 Jun 27. Epub 2022 Jun 27.

School of Chemistry, Xi'an Jiaotong University, Xi'an 710049, P. R. China.

Amide synthesis is one of the most important transformations in organic chemistry due to the broad application in pharmaceutical drugs and organic materials. In this report, we describe a mild protocol for amide formation using the readily available nitroarenes as nitrogen sources and an inexpensive iron complex as a catalyst. Because of the use of the pH-neutral conditions and the avoidance of the strong oxidant or reductant, a wide range of aromatic and aliphatic aldehydes as well as nitroarenes with various functional groups could be tolerated well. A plausible mechanism is proposed based on the detailed studies, in which iron catalyst initiates the radical process and the solvent plays a key role as O-atom acceptor.
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http://dx.doi.org/10.1021/acs.orglett.2c01743DOI Listing
June 2022

Construction of multifunctional wound dressings with their application in chronic wound treatment.

Biomater Sci 2022 Jun 27. Epub 2022 Jun 27.

National Engineering Research Center for Biomaterials, Chuanda-Jinbo Joint Research Center, Sichuan University, Chengdu, 610064, China.

As the prevalence of diabetes increases year by year and the aging population continues to intensify in the world, chronic wounds such as diabetic foot ulcers and pressure ulcers have become serious problems that threaten people's health, and have brought an enormous burden to the world healthcare system. Conventional clinical treatment of chronic wounds relies on non-specific topical care (including debridement, infection/inflammation control, and frequent wound dressing changes), which can alleviate disease progression and reduce patient suffering to a certain extent, but the overall cure rate is less than 50% and the recurrence rate is high. Traditional wound dressings such as gauze, hydrocolloids, films and foams are single-function, acting as a physical barrier or absorbing exudates, and cannot meet all the needs of the entire chronic wound healing process. Recently, a large number of novel functional dressings have been reported for chronic wound repair. Based on the progress on wound dressings in recent years and the relevant research experience of our group, the review summarizes and discusses the progress on multifunctional wound dressings (such as microneedles, sponges and hydrogels) with anti-inflammatory, antioxidant, antibacterial, pro-angiogenic and tissue adhesive functions in detail. At the same time, the various responsive mechanisms ( microenvironment or stimulation) of the smart multifunctional wound dressing are also analyzed in detail. It is expected that the review could provide some inspiration and suggestions for research on dressings for chronic wound treatment.
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http://dx.doi.org/10.1039/d2bm00620kDOI Listing
June 2022

Cystatin C-based estimated GFR performs best in identifying individuals with poorer survival in an unselected Chinese population: results from the China Health and Retirement Longitudinal Study (CHARLS).

Clin Kidney J 2022 Jul 7;15(7):1322-1332. Epub 2022 Mar 7.

Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: The decline in estimated glomerular filtration rate (eGFR) has been reported as a risk factor for mortality. However, it remains unclear which eGFR equation is most useful in predicting death in the general Chinese population.

Methods: The association was examined between eGFR and all-cause mortality using data from the China Health and Retirement Longitudinal Study. Participants with complete data in 2011 and survival follow-up in 2013, 2015 and 2018 were included and analyzed in three separate cohorts, which included 8160, 8154 and 8020 participants, respectively. Logistic regression analyses, receiver operating characteristic curve, continuous net reclassification improvement (NRI) and integrated discrimination improvement (IDI) were computed to compare the discriminative power of eGFR derived by abbreviated Modification of Diet in Renal Disease (MDRD), Chinese coefficient-modified MDRD (MDRD-CN), Japanese coefficient-modified MDRD (MDRD-JPN), CKD-EPI, Japanese coefficient-modified CKD-EPI (CKD-EPI-JPN), CKD-EPI, CKD-EPI, CKD-EPI fit without race and CKD-EPI fit without race.

Results: A decreased eGFR (<60 ml/min/1.73 m) was significantly associated with increased mortality at 2 years no matter which eGFR equation was used (odds ratio ranged between 2.02 and 4.94, all P < 0.001). The association remained significant after adjusting multiple covariates when MDRD-CN, CKD-EPI or CKD-EPI fit without race was used. CKD-EPI showed the highest discriminative power for mortality (area under the curve 0.744 ± 0.40) and outperformed other equations (all P < 0.001) except for CKD-EPI. The overall risk classification was also improved when the CKD-EPI equation was adopted as indicated by continuous NRI and IDI. Similar results were observed at 4 and 7 years.

Conclusions: A decline in eGFR by all equations could predict poorer survival, among which the CKD-EPI equation showed the best discriminative power.
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http://dx.doi.org/10.1093/ckj/sfac070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9217656PMC
July 2022

Founders' Creativity, Business Model Innovation, and Business Growth.

Front Psychol 2022 10;13:892716. Epub 2022 Jun 10.

School of Management, Jilin University, Changchun, China.

Given the existing studies on the role of different founders' personalities in the growth of new ventures, we take the digital technology start-ups as the research object and focus on the role of founders' creativity. In this study, we assess the relationship between founders' creativity and business growth. According to the framework of upper echelons theory, we propose several hypotheses. Based on the investigation of 153 new ventures in China's transition economy, we find that: (1) founders' creativity has a positive impact on the growth of enterprises; (2) business model innovation positively mediates the relationship between founders' creativity and enterprise growth; (3) work experience is found to be a moderator of the relationship between founders' creativity and business model innovation. The conclusion of our analysis not only helps to further deepen the research on the growth process of start-ups but also helps to further expand the important role of business model innovation and founders' work experience in the growth of new firms.
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http://dx.doi.org/10.3389/fpsyg.2022.892716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226674PMC
June 2022

Bacteriophage-Resistant Mutant of Is Impaired in Biofilm Formation.

Front Microbiol 2022 9;13:913023. Epub 2022 Jun 9.

Department of Microbiology, Army Medical University, Chongqing, China.

is a common gram-positive non-spore-forming bacterium in nature and is found in the upper respiratory tract, intestine, and mouth of healthy people. is also one of the common pathogens causing nosocomial infections and is resistant to several antibiotics commonly used in practice. Thus, treating drug-resistant with antibiotics is challenging, and new approaches are needed. In this study, we isolated a bacteriophage named EFap02 that targets strain EFa02 from sewage at Southwest Hospital. Phage EFap02 belongs to the family with a long tail of approximately 210 nm, and EFap02 can tolerate a strong acid and alkali environment and high temperature. Its receptor was identified as the capsular polysaccharide. Phage-resistant mutants had loss-of-function mutations in glycosyltransferase (), which is responsible for capsular polysaccharide biosynthesis, and this caused the loss of capsular polysaccharide and interruption of phage adsorption. Although phage-resistant mutants against EFap02 can be selected, such mutants are impaired in biofilm formation due to the loss of capsular polysaccharide, which compromises its virulence. Therefore, this study provided a detailed description of the EFap02 phage with the potential for treating infection.
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http://dx.doi.org/10.3389/fmicb.2022.913023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218719PMC
June 2022

Comparison of Intestinal Microbes in Noninfectious Anterior Scleritis Patients With and Without Rheumatoid Arthritis.

Front Microbiol 2022 9;13:925929. Epub 2022 Jun 9.

Ophthalmologic Center of the Second Hospital, Jilin University, Changchun, China.

We compared intestinal microbes in anterior noninfectious scleritis patients with and without rheumatoid arthritis. Active noninfectious anterior scleritis patients without other immune diseases (G group, 16 patients) or with active rheumatoid arthritis (GY group, seven patients) were included in this study. Eight age- and sex-matched healthy subjects served as controls (N group). DNA was extracted from fecal samples. The V3-V4 16S rDNA region was amplified and sequenced by high-throughput 16S rDNA analysis, and microbial contents were determined. A significant decrease in species richness in the GY group was revealed by α- and β-diversity analyses ( = 0.02 and  = 0.004, respectively). At the genus level, 14 enriched and 10 decreased microbes in the G group and 13 enriched and 18 decreased microbes in the GY group were identified. Among them, four microbes were enriched in both the G and GY groups, including , , , and . Although two microbes ( and ) exhibited similar tendencies in the G and GY groups, changes in these microbes were more significant in the GY group ( < 0.05). Interaction analysis showed that , , and , which were enriched in both the G and GY groups, correlated positively with each other. In addition, nine microbes were decreased in the GY group, which demonstrates a potential protective role for these microbes in the pathogenesis of scleritis interactions with each other.
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http://dx.doi.org/10.3389/fmicb.2022.925929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9218904PMC
June 2022

Leakage Current Sensor and Neural Network for  MOA Monitoring.

Comput Intell Neurosci 2022 17;2022:6728900. Epub 2022 Jun 17.

Ma'anshan Electric Power Supply Company, State Grid Anhui Electric Power Co Ltd, Ma'anshan 243000, China.

Metal-oxide arrester (MOA) has been widely used in electric power systems. The leakage current monitoring of MOA can not only detect the MOA's running state continuously and intelligently but also reduce the unexpected outage of the equipment, which is also beneficial to the stability of the grid. The MOA loses its protection function due to various faults caused by excessive leakage current in actual running. This article studies the monitoring method of MOA based on leakage current sensor and back propagation (BP) neural network. At first, we design a novel leakage current sensor to acquire the leakage current of MOA. Then, the leakage current measurement of MOA based on harmonic analysis is proposed. Finally, the strong training ability of the BP neural network is used to train some key parameters that can reflect the aging of MOA so as to monitor the MOA state. The experimental results show that the leakage current acquired from the simulation is close to the actual leakage current that needs to be measured. It is also shown that the proposed method has good anti-interference and can effectively monitor the aging of MOA. Through the training of the BP neural network, the experiments prove that the training method in this article is superior to other neural network training methods obviously.
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http://dx.doi.org/10.1155/2022/6728900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232350PMC
June 2022

Developing computable sustainable urbanization science: interdisciplinary perspective.

Comput Urban Sci 2022 20;2(1):17. Epub 2022 Jun 20.

Institute of Geographic Sciences and Natural Resources Research, Key Laboratory of Regional Sustainable Development Modeling, Chinese Academy of Sciences, Beijing, 100101 China.

In this era of drastic global change, the Anthropocene, carbon neutrality and sustainable development have become common twenty-first century human challenges and goals. Large-scale urbanization is indicative of human activities and provides an important impetus for environmental changes; therefore, cities have become an important stage in which to promote a more sustainable future development of human society. However, current researchers study urbanization issues based on the perspectives and tools of their respective disciplines; therefore, a holistic and comprehensive understanding of urbanization is lacking due to the insufficient integration of multidisciplinary study perspectives. We explored the construction of interdisciplinary computable sustainable urbanization and introduces a conceptual framework for interdisciplinary urbanization, as scientific computing supports and integrates the natural sciences and humanities to simulate urban evolution and further observe, explain, and optimize human and environment interactions in urban areas. We advocated for the establishment of major international research programs and organizations in the field of sustainable urbanization, and the cultivation of talented young professionals with broad-ranging interdisciplinary interests. Expectantly, we hope a livable planet in the Anthropocene era could be created by developing sustainable urbanization and achieving carbon neutrality.
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http://dx.doi.org/10.1007/s43762-022-00048-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207868PMC
June 2022

The Gene Has No Negative Effect on Wheat Agronomic Performance: Evidence From Newly Established Near-Isogenic Lines.

Front Plant Sci 2022 8;13:918559. Epub 2022 Jun 8.

The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

Wheat genotypes resistant to powdery mildew ( f. sp. , ) provide a sustainable means for disease control. We developed a pair of near-isogenic lines H962R and H962S with contrasting reactions to powdery mildew from a residue heterozygous line. H962R was resistant to 127 out of the 136 isolates collected from the major wheat-producing regions of China and showed a similar virulence/avirulence pattern as Fuzhuang 30, Xiaobaidong, and Hongquanmang carrying resistance allele of , but H962S was resistant to none of them. A dominant gene was responsible for the powdery mildew resistance of H962R as revealed by the genetic analysis using segregating populations derived from a cross between H962R and H962S. Molecular marker analysis detected a resistance locus, designated , on a genetic interval of the chromosome arm 7BL where resides. This locus was co-segregated with the functional marker of . The PCR-based sequence alignment of demonstrated that H962R had an identical sequence as Fuzhuang 30 (haplotype HapGA), and H962S possessed the same sequence as the powdery mildew susceptible cultivar Kenong 199. The genomic compositions of lines H962R and H962S were highly comparable as evidenced by only a small percentage of SNP variations detected by the 16K Genotyping by Target Sequencing (GBTS) SNP array and the 90K Illumina iSelect Wheat SNP array. The two lines performed similarly in the yield-related and plant growth traits investigated, except for greater kernel weight in H962R than in H962S. This indicates that has no deleterious effect and can be served as an excellent disease resistance gene in wheat breeding.
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http://dx.doi.org/10.3389/fpls.2022.918559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9216190PMC
June 2022

IFT20 governs mesenchymal stem cell fate through positively regulating TGF-β-Smad2/3-Glut1 signaling mediated glucose metabolism.

Redox Biol 2022 Jun 20;54:102373. Epub 2022 Jun 20.

Department of Basic & Translational Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA; Department of Periodontics, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA; The Penn Center for Musculoskeletal Disorders, School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA. Electronic address:

Aberrant lineage allocation of mesenchymal stem cells (MSCs) could cause bone marrow osteoblast-adipocyte imbalance, and glucose as an important nutrient is required for the maintenance of the MSCs' fate and function. Intraflagellar transport 20 (IFT20) is one of the IFT complex B protein which regulates osteoblast differentiation, and bone formation, but how IFT20 regulates MSCs' fate remains undefined. Here, we demonstrated that IFT20 controls MSC lineage allocation through regulating glucose metabolism during skeletal development. IFT20 deficiency in the early stage of MSCs caused significantly shortened limbs, decreased bone mass and significant increase in marrow fat. However, deletion of IFT20 in the later stage of MSCs and osteocytes just slightly decreased bone mass and bone growth and increased marrow fat. Additionally, we found that loss of IFT20 in MSCs promotes adipocyte formation, which enhances RANKL expression and bone resorption. Conversely, ablation of IFT20 in adipocytes reversed these phenotypes. Mechanistically, loss of IFT20 in MSCs significantly decreased glucose tolerance and suppressed glucose uptake and lactate and ATP production. Moreover, loss of IFT20 significantly decreased the activity of TGF-β-Smad2/3 signaling and reduced the binding activity of Smad2/3 to Glut1 promoter to downregulate Glut1 expression. These findings indicate that IFT20 plays essential roles for preventing MSC lineage allocation into adipocytes through TGF-β-Smad2/3-Glut1 axis.
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http://dx.doi.org/10.1016/j.redox.2022.102373DOI Listing
June 2022

Derivation of copper water quality criteria in the Bohai Sea of China considering the effects of multiple environmental factors on copper toxicity.

Environ Pollut 2022 Jun 21;308:119666. Epub 2022 Jun 21.

Engineering Research Center of Seawater Utilization of Ministry of Education, School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, 300130, China; Hebei Collaborative Innovation Center of Modern Marine Chemical Technology, Tianjin, 300130, China. Electronic address:

Copper has become one of the most important heavy metal pollutants in the environment because of its wide application and high toxicity, but research on water quality criteria (WQCs) on copper is limited, especially the derivation of seawater WQC. In addition, the toxicity of copper in the seawater system is affected by various environmental factors. Therefore, establishing a WQC that meets the characteristics of the regional environment is a top priority. The correlations between four factors of temperature, salinity, pH, dissolved organic carbon (DOC) and the toxic effect values of copper were analyzed in this study, and the temperature was determined as the most influential factor among the four factors in the Bohai Sea. A specific correlation between temperature and the toxic effects of copper was identified, and WQCs were derived based on the identified correlation and the variations of the Bohai Sea's temperature in different seasons by species sensitivity distribution (SSD) method. Under the condition of the winter, spring, autumn, and summer with an average water temperature of 0.09, 15.96, 17.83, and 24.87 °C, the obtained short-term water quality criteria (SWQCs) were 44.29, 4.70, 4.31, and 3.33 μg/L; the long-term water quality criteria (LWQCs) were 18.14, 1.93, 1.77 and 1.36 μg/L. The findings indicated the importance of introducing specific environmental conditions during the derivation process. This work could provide valuable information for pollution prevention and aquatic life protection in the Bohai Sea and provide a valuable reference for the derivation of criteria in other regions alike.
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http://dx.doi.org/10.1016/j.envpol.2022.119666DOI Listing
June 2022

New lignans and phenylethanoid with antioxidant activity from aerial parts of (Thunb.) Vahl.

Nat Prod Res 2022 Jun 24:1-9. Epub 2022 Jun 24.

Department of Medicinal Natural Products, West China School of Pharmacy, Sichuan University, Chengdu, China.

Two new lignans, phillyroside A() and phillyroside B(), together with three new phenylethanoid glycoside, forsythoside K(), forsythoside L() and forsythol L (), while compounds was an aglycon of forsythoside L(), were isolated from the aerial parts of (Thunb.) Vahl. Their structures were elucidated by comprehensive analyses of standard spectroscopic data (MS, IR, and NMR) and the in vitro antioxidant activity of five new compounds were evaluated in DPPH and ABTS radical scavenging experiment and ferric reducing ability of plasma (FRAP) experiment. Compounds and exhibited antioxidant activity with IC values ranging from 112.49 to 153.58 μM in DPPH experiment and 45.43 to 64.09 μM in ABTS experiment.
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http://dx.doi.org/10.1080/14786419.2022.2087650DOI Listing
June 2022

Peking Prognostic Score, Based on Preoperative Sarcopenia Status, Is a Novel Prognostic Factor in Patients With Gastric Cancer.

Front Nutr 2022 6;9:910271. Epub 2022 Jun 6.

Department of Pancreatic and Gastric Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: This study focused on assessing the role of the Peking prognostic score (PPS), a novel prognostic index based on muscle atrophy and lymphocyte-to-C-reactive protein ratio, within gastric cancer patient prognosis.

Methods: We analyzed the data collected from 774 gastric cancer cases between April 2011 and February 2016 (discovery cohort). The results were assessed in 575 gastric cancer cases from March 2016 to September 2019 (validation cohort). For evaluating skeletal muscle mass, we obtained computed tomography images at the third lumbar vertebra level (L3). We performed a time-dependent receiver operating characteristic curve (t-ROC) to analyze PPS's prognostic significance with others.

Results: The discovery cohort enrolled altogether 774 patients with non-metastatic gastric cancer, including 639 (82.5%) men along with 135 (17.5%) women. The patients were divided into 3 groups; 166 patients (21.4%) were assigned into group 0, 472 (60.9%) in group 1, and 136 (17.7%) in group 2, respectively. An increased PPS was in direct proportion to an elder age, reduced body mass index, higher Pathological Tumor Lymph Node Metastasis stage, perineural invasion, and vascular invasion. We identified PPS to independently estimate patient overall survival (OS) together with disease-free survival (DFS; both < 0.001). Additionally, as revealed by t-ROC analysis, PPS exhibited the highest sensitivity compared with other prognostic scoring systems in predicting patient survival. Finally, we evaluated the prognostic value of PPS in the validation cohort and confirmed that preoperative PPS independently estimates patient OS and DFS.

Conclusion: The PPS accounts for an efficient nutrition-inflammation prognostic scoring system in gastric cancer patients.
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http://dx.doi.org/10.3389/fnut.2022.910271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210445PMC
June 2022

Machine Learning of Dose-Volume Histogram Parameters Predicting Overall Survival in Patients with Cervical Cancer Treated with Definitive Radiotherapy.

J Oncol 2022 14;2022:2643376. Epub 2022 Jun 14.

Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.

Purpose: To analyze the effects of dosimetric parameters and clinical characteristics on overall survival (OS) by machine learning algorithms.

Methods And Materials: 128 patients with cervical cancer were treated with definitive pelvic radiotherapy with or without chemotherapy followed by image-guided brachytherapy. The elastic-net models with integrating DVH parameters and baseline clinical factors, only DVH parameters and only baseline clinical factors were constructed in 5-folds cross-validations for 100 iteration bootstrapping, and then were compared using concordance index (C-index) criteria. Finally, the selected important factors were used to build multivariable Cox-pH models for OS and also shown in nomograms for clinical usage.

Results: The median OS occurred was 25.78 months with 25 (19.53%) deaths. The elastic-net models integrating clinical and DVH factors had the best prediction performances (C-index 0.76 in the train set and C-index 0.74 in the test set). Three important factors were selected, including baseline hemoglobin level as the protective factor, primary tumor volume (GTV_P) volume, and body V5 as the risk factors. The final multivariable Cox-pH models were constructed using these important factors and had prediction performance (C-index: 0.78, 95%CI: 0.73-0.81).

Conclusions: This is the first attempt to establish elastic-net models to study the contributions of DVH parameters for predicting OS in patients with cervical cancer. These results can facilitate individualized tailoring of radiation treatment in cervical cancer patients.
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http://dx.doi.org/10.1155/2022/2643376DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213181PMC
June 2022

Genome-Wide Reassortment Analysis of Influenza A H7N9 Viruses Circulating in China during 2013-2019.

Viruses 2022 Jun 9;14(6). Epub 2022 Jun 9.

Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.

Reassortment with the H9N2 virus gave rise to the zoonotic H7N9 avian influenza virus (AIV), which caused more than five outbreak waves in humans, with high mortality. The frequent exchange of genomic segments between H7N9 and H9N2 has been well-documented. However, the reassortment patterns have not been described and are not yet fully understood. Here, we used phylogenetic analyses to investigate the patterns of intersubtype and intrasubtype/intralineage reassortment across the eight viral segments. The H7N9 virus and its progeny frequently exchanged internal genes with the H9N2 virus but rarely with the other AIV subtypes. Before beginning the intrasubtype/intralineage reassortment analyses, five Yangtze River Delta (YRD A-E) and two Pearl River Delta (PRD A-B) clusters were divided according to the HA gene phylogeny. The seven reset segment genes were also nomenclatured consistently. As revealed by the tanglegram results, high intralineage reassortment rates were determined in waves 2-3 and 5. Additionally, the clusters of PB2 c05 and M c02 were the most dominant in wave 5, which could have contributed to the onset of the largest H7N9 outbreak in 2016-2017. Meanwhile, a portion of the YRD-C cluster (HP H7N9) inherited their PB2, PA, and M segments from the co-circulating YRD-E (LP H7N9) cluster during wave 5. Untanglegram results revealed that the reassortment rate between HA and NA was lower than HA with any of the other six segments. A multidimensional scaling plot revealed a robust genetic linkage between the PB2 and PA genes, indicating that they may share a co-evolutionary history. Furthermore, we observed relatively more robust positive selection pressure on HA, NA, M2, and NS1 proteins. Our findings demonstrate that frequent reassortment, particular reassorted patterns, and adaptive mutations shaped the H7N9 viral genetic diversity and evolution. Increased surveillance is required immediately to better understand the current state of the HP H7N9 AIV.
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http://dx.doi.org/10.3390/v14061256DOI Listing
June 2022

Research on Multi-Sensor Fusion Indoor Fire Perception Algorithm Based on Improved TCN.

Sensors (Basel) 2022 Jun 16;22(12). Epub 2022 Jun 16.

Tianjin Key Laboratory of Electronic Materials and Devices, School of Electronics and Information Engineering, Hebei University of Technology, Tianjin 300401, China.

Indoor fires cause huge casualties and economic losses worldwide. Thus, it is critical to quickly and accurately perceive the fire. In this work, an indoor fire perception algorithm based on multi-sensor fusion was proposed. Firstly, the sensor data features were fully extracted by improved temporal convolutional network (TCN). Then, the dimension of the extracted features was reduced by adaptive average pooling (AAP). Finally, the fire classification was realized by the support vector machine (SVM) classifier. Experimental results demonstrated that the proposed algorithm can improve accuracy of fire classification by more than 2.5% and detection speed by more than 15%, compared with TCN, back propagation (BP) neural network and long short-term memory (LSTM). In conclusion, the proposed algorithm can perceive the fire quickly and accurately, which is of great significance to improve the performance of the current fire prediction systems.
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http://dx.doi.org/10.3390/s22124550DOI Listing
June 2022

Design and Implementation of Real-Time Localization System (RTLS) Based on UWB and TDoA Algorithm.

Sensors (Basel) 2022 Jun 8;22(12). Epub 2022 Jun 8.

Science and Technology on Near-Surface Detection Laboratory, Wuxi 214000, China.

Nowadays, accurate localization plays an essential role in many fields, such as target tracking and path planning. The challenges of indoor localization include inadequate localization accuracy, unreasonable anchor deployment in complex scenarios, lack of stability, and the high cost. So, the universal positioning technologies cannot meet the real application requirements scarcely. To overcome these shortcomings, a comprehensive ultra wide-band (UWB)-based real-time localization system (RTLS) is presented in this paper. We introduce the architecture of a real-time localization system, then propose a new wireless clock synchronization (WCS) scheme, and finally discuss the time difference of arrival (TDoA) algorithm. We define the time-base selection strategy for the TDoA algorithm, and we analyze the relationship between anchor deployment and positioning accuracy. The extended Kalman filter (EKF) method is presented for non-linear dynamic localization estimation, and it performs well in terms of stability and accuracy on moving targets.
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http://dx.doi.org/10.3390/s22124353DOI Listing
June 2022

The Intelligent Path Planning System of Agricultural Robot via Reinforcement Learning.

Sensors (Basel) 2022 Jun 7;22(12). Epub 2022 Jun 7.

School of Electrical and Information Engineering, Tianjin University, Tianjin 300072, China.

Agricultural robots are one of the important means to promote agricultural modernization and improve agricultural efficiency. With the development of artificial intelligence technology and the maturity of Internet of Things (IoT) technology, people put forward higher requirements for the intelligence of robots. Agricultural robots must have intelligent control functions in agricultural scenarios and be able to autonomously decide paths to complete agricultural tasks. In response to this requirement, this paper proposes a Residual-like Soft Actor Critic (R-SAC) algorithm for agricultural scenarios to realize safe obstacle avoidance and intelligent path planning of robots. In addition, in order to alleviate the time-consuming problem of exploration process of reinforcement learning, this paper proposes an offline expert experience pre-training method, which improves the training efficiency of reinforcement learning. Moreover, this paper optimizes the reward mechanism of the algorithm by using multi-step TD-error, which solves the probable dilemma during training. Experiments verify that our proposed method has stable performance in both static and dynamic obstacle environments, and is superior to other reinforcement learning algorithms. It is a stable and efficient path planning method and has visible application potential in agricultural robots.
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http://dx.doi.org/10.3390/s22124316DOI Listing
June 2022

Design and Synthesis of a Mitochondria-Targeting Radioprotectant for Promoting Skin Wound Healing Combined with Ionizing Radiation Injury.

Pharmaceuticals (Basel) 2022 Jun 6;15(6). Epub 2022 Jun 6.

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University (Army Medical University), Chongqing 400038, China.

Wound healing is seriously retarded when combined with ionizing radiation injury, because radiation-induced excessive reactive oxygen species (ROS) profoundly affect cell growth and wound healing. Mitochondria play vital roles not only as cellular energy factories but also as the main source of endogenous ROS, and in this work a mitochondria-targeting radioprotectant (CY-TMP1) is reported for radiation injury-combined wound repair. It was designed, synthesized and screened out from different conjugates between mitochondria-targeting heptamethine cyanine dyes and a peroxidation inhibitor 2,2,6,6-tetramethylpiperidinyloxy (TEMPO). CY-TMP1 specifically accumulated in mitochondria, efficiently mitigated mitochondrial ROS and total intracellular ROS induced by 6 Gy of X-ray ionizing irradiation, thereby exhibiting a notable radioprotective effect. The mechanism study further demonstrated that CY-TMP1 protected mitochondria from radiation-induced injury, including maintaining mitochondrial membrane potential (MMP) and ATP generation, thereby reducing the ratio of cell apoptotic death. Particularly, an in vivo experiment showed that CY-TMP1 could effectively accelerate wound closure of mice after 6 Gy of whole-body ionizing radiation. Immunohistochemical staining further indicated that CY-TMP1 may improve wound repair through angiogenesis and re-epithelialization. Therefore, mitochondria-targeting ROS scavengers may present a feasible strategy to conquer refractory wound combined with radiation injury.
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http://dx.doi.org/10.3390/ph15060721DOI Listing
June 2022
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