Publications by authors named "Li Xu"

4,780 Publications

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How do controlled-release fertilizer coated microplastics dynamically affect Cd availability by regulating Fe species and DOC content in soil?

Sci Total Environ 2022 Aug 8:157886. Epub 2022 Aug 8.

Institute of Plan Nutrition, Resources and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China; Beijing Engineering Technology Research Center for Slow / Controlled-Release Fertilizer, Beijing 100097, China. Electronic address:

Microplastics (MPs) affect the accumulation of heavy metals by regulating the soil environment. However, studies on the dynamic effects of microplastics on the available states of heavy metals in soil are lacking. In particular, how controlled-release fertilizer coated microplastics can synergistically change the avsilable states of heavy metals in soil by affecting soil physical and chemical properties and microbial community structure is still lacking. The dynamic effect of polyurethane (PU) MPs on the effective state of soil cadmium (Cd; DGTCd), at different particle sizes and concentrations, was studied in situ by diffusive gradient in thin-films (DGT) for the first time. The bioavailability, soil chemical properties, and microbial effects of PU MPs on Cd depend on PU particle size and concentration; high-concentration (1 %) PU MPs cause a significant increase in DGT-Cd concentration. The addition of PU MPs decreased soil pH and dissolved oxygen content (DOC), while increasing the absolute zeta value, Fe(II) and Mn(II), in a manner dependent on particle size, concentration, and culture time. Correlation analysis combined with path analysis showed that PU MPs affected the effective state of Cd by changing soil properties, among which Fe(II) content and DOC were important factors controlling the activation of Cd. Meanwhile, changes in soil properties and heavy metal availability correlated significantly with microbial community composition, suggesting that PU MPs may indirectly impact heavy metal activity by affecting microorganisms and functional genes associated with C and Fe cycling. Therefore, when the concentration of PU MPs is higher than 1 %, we should strengthen ecological risk prevention and control of the compound pollution of controlled-release fertilizer coated microplastics and heavy metals in farmland soil.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157886DOI Listing
August 2022

Preoperative Transthoracic Echocardiography Predicts Cardiac Complications in Elderly Patients with Coronary Artery Disease Undergoing Noncardiac Surgery.

Clin Interv Aging 2022 2;17:1151-1161. Epub 2022 Aug 2.

Department of Anesthesiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China.

Purpose: Guidelines have not recommended routine transthoracic echocardiography (TTE) for elderly patients prior to noncardiac surgery. We aimed to evaluate the significance of preoperative TTE to predict perioperative cardiac complications (PCCs) for elderly patients with coronary artery disease (CAD) undergoing noncardiac surgery.

Patients And Methods: We retrospectively reviewed 2204 patients over 65 years of age with CAD who underwent TTE before intermediate- or high-risk noncardiac surgery in a teaching hospital in China between September 2013 and August 2019. The revised cardiac risk index (RCRI) was assessed. PCCs comprised acute coronary syndrome, heart failure, new-onset severe arrhythmia, nonfatal cardiac arrest, and cardiac death. Logistic regression was used to build the prediction model for PCCs. Discrimination was evaluated using receiver operating characteristic curves, and a nomogram of the predictive model was constructed.

Results: PCCs occurred in 189 (8.6%) patients. Multivariable analysis showed that eight clinical risk factors (age, history of myocardial infarction, insulin therapy for diabetes, New York Heart Association classification, preoperative serum creatinine, preoperative electrocardiogram ST-T abnormality and pathological Q wave, and American Society of Anesthesiologists classification) and five TTE parameters (left atrial anteroposterior dimension, left ventricular ejection fraction, left ventricular diastolic dysfunction, pulmonary hypertension, and regional ventricular wall motion abnormality) were associated with PCCs. The receiver operating characteristic curve for the clinical plus TTE model provided better discrimination for PCCs compared with the RCRI model (area under the curve: 0.731 vs 0.564; < 0.001) and the clinical model (area under the curve: 0.731 vs 0.697, = 0.001), respectively. The clinical plus TTE model was presented as a prognostic nomogram.

Conclusion: Preoperative TTE may help predict PCCs in elderly patients with CAD undergoing noncardiac surgery, and the prognostic nomogram from this study appeared to be useful for the assessment of perioperative cardiac risk.
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http://dx.doi.org/10.2147/CIA.S369657DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356610PMC
August 2022

The anti-inflammatory effects of Hedyotis diffusa Willd on SLE with STAT3 as a key target.

J Ethnopharmacol 2022 Aug 5:115597. Epub 2022 Aug 5.

College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang, PR China. Electronic address:

Ethnopharmacological Relevance: Hedyotis diffusa Willd, also named Scleromitrion diffusum (Willd.) R.J. Wang, is one medical herb, which has been traditionally used by the She nationality in China. And H. diffusa represents a beneficial effect on Systemic lupus erythematosus (SLE) treatment in clinic.

Aim Of The Study: The underlying mechanisms of the protective effects of H. diffusa on SLE remain unclear. In this study, we treated MRL/lpr mice with H. diffusa water extract (HDW) to assess its therapeutic effects and verified its regulating signalling pathway through cytological experiments.

Materials And Methods: In the present study, the constituents of HDW were analysed through ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and SCIEX OS software. The protective activity and underlying mechanisms were studied in a MRL/lpr lupus mouse model. The blood cells, autoantibodies, metabolites and the cytokines in serum were identified with a hematology analyzer, specific ELISA kit, GC/MS system and cytometric assays. The histological and immunohistochemical analysis were engaged in the morphologic, and the expression and translocation of the crucial protein observation. The dual luciferase reporter assay was applied to identifying the regulative activity of HDW. The transcription and translation expression of the protein was studied by real-time PCR and western blot assays. The network pharmacology analysis was employed to predict the IL-6/STAT3 pathway regulators and the screen the STAT3 inhibitors in HDW.

Results: The results revealed the capability of HDW to attenuate the production of autoantibodies, secretion of inflammatory cytokines (IL-6 and IFN-γ), and suppressed the IgG and C3 deposition, the development of glomerular lesions in MRL/lpr mice. Serum metabolomics study showed the improvement in serum metabolites, especially aminoacyl-tRNA biosynthesis, by HDW. IL-6 was clarified to be highly associated with the significantly changed metabolites in network analysis. We further demonstrated the effects of HDW on the IL-6/STAT3 pathway in vivo and in vitro.

Conclusions: This study suggested that HDW exerts a therapeutic effect in SLE model mice by suppressing the IL-6/STAT3 pathway.
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http://dx.doi.org/10.1016/j.jep.2022.115597DOI Listing
August 2022

Greater bone marrow fat and myosteatosis are associated with lower vBMD but not asymptomatic vertebral fracture.

Eur Radiol 2022 Aug 6. Epub 2022 Aug 6.

Department of Radiology, Beijing Jishuitan Hospital, Beijing, China.

Objectives: Organ fat may affect bone metabolism and be associated with vertebral fracture (VF). This study aimed to explore relationships between VF, adiposity indexes measured by MRI, and volumetric BMD (vBMD) measured by quantitative CT (QCT).

Methods: Four hundred volunteers, ranging in age from 22 to 83 years, were recruited and underwent same-day abdominal QCT and chemical shift-encoded (CSE) MRI. We used MRI to quantify the fat content of bone marrow (BMF), psoas major and paraspinal muscles, and the liver. Abdominal fat, VF, and vBMD of the lumbar spine were measured by QCT. For VF discrimination analysis, we examined both the whole cohort (60 VF cases in 30 men and 30 women) and a restricted subgroup of those aged over 50 years (50 VF cases in 23 men and 27 women).

Results: Amongst the men, a 1 SD increase in BMF was associated with a 27.67 (95% CI, -32.71 to -22.62) mg/cm decrease in vBMD after adjusting for age and BMI. Amongst women, all adiposity indexes except for liver fat were significantly associated with vBMD, with BMF having the strongest association (β, -24.00; 95% CI, -28.54 to -19.46 mg/cm). Similar findings were also observed in participants aged over 50 years. The associations of adiposity indexes with vertebral fracture were not significant after adjusting for age in both sexes aged over 50 years.

Conclusions: In both sexes, higher bone marrow fat was associated with lower vBMD at the spine. However, marrow fat and other adipose tissues were not associated with radiographic-based prevalent vertebral fractures.

Key Points: • In both sexes, higher bone marrow fat was associated with lower vBMD at the spine. • Among women, all adiposity indexes except for liver fat content were significantly associated with vBMD, with bone marrow fat having the strongest association. • Marrow fat and other adipose tissues were not associated with radiographic-based asymptomatic vertebral fractures.
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http://dx.doi.org/10.1007/s00330-022-08979-xDOI Listing
August 2022

Sustained delivery of gemcitabine injectable mussel-inspired hydrogels for the local therapy of pancreatic cancer.

J Mater Chem B 2022 Aug 5. Epub 2022 Aug 5.

State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

The issue of pervasively enhanced drug resistance of pancreatic cancer is fundamental to a better understanding of gemcitabine-based chemotherapy. Currently available treatment plans involving injectable therapeutics are mainly engineered to improve the performance and broaden their applications in the domain of biomedicine. Fixed-dose-rate infusion of free gemcitabine (Gem) has drawn appropriate attention for its potent anti-tumor efficacy against various solid tumors, whereas it remains a considerable challenge to extend its application and achieve better treatment. Here, we have prepared and demonstrated a long-acting delivery system using gemcitabine and injectable hydrogel for the localized treatment of pancreatic cancer. The hydrogel was prepared using polysaccharide derivatives, oxidized-carboxymethylcellulose (OCMC) and carboxymethylchitosan (CMCS) at optimal ratios by a dopamine-functionalized method for the controlled release of Gem. drug release behaviors for up to a week indicated sustained drug release of the Gem delivery system. Moreover, desirable apoptosis promotion and apparent cellular proliferation inhibition associated with the drug depot have been found against BxPC-3 pancreatic cancer cells, bringing minimized side effects to systemic normal tissues. The current findings manifested that the release out of the localized delivery platform in a sustained pattern afforded a durable gemcitabine-based chemotherapy effect and inhibited tumor metastasis more persistently after intratumoral injection of the [email protected] system, thereby advancing the development of novel drug-loaded materials with properties not accessed previously.
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http://dx.doi.org/10.1039/d1tb02858hDOI Listing
August 2022

Salidroside Inhibits Ischemia/Reperfusion-Induced Myocardial Apoptosis by Targeting Mir-378a-3p Via the Igf1r/Pi3k/Akt Signaling Pathway.

Transplant Proc 2022 Aug 1. Epub 2022 Aug 1.

Jiangsu Kangyuan Pharmaceutical Co, Ltd, Lianyungang, China; State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Jiangsu Lianyungang, China. Electronic address:

Background: The present study aimed to investigate the protective effects and mechanism of salidroside (SAL) on hypoxia/reoxygenation (H/R)-induced cardiomyocyte apoptosis and myocardial ischemia/reperfusion (I/R) injury.

Methods: We set up an H/R H9c2 cell model in vitro and an I/R rat model in vivo. Cell viability, apoptosis and histopathologic evaluation were conducted.

Results: The cell viability of H/R-induced cardiomyocytes was increased by pretreatment of SAL, whereas the release of lactate dehydrogenase, reactive oxygen species production, and apoptosis were decreased accompanied with reduced Cleaved-caspase-3 and Bax, and increased Bcl-2 expressions. The SAL restored mitochondrial membrane potential both in vitro and in vivo, and improved electrocardiographic abnormality, and attenuated myocardial apoptosis and injury in I/R-induced rats. The transfection of miR-378a-3p inhibitor counteracted the effects of SAL-induced increase of cell viability and decrease of cell apoptosis and mitochondrial membrane potential. SAL reduced the expression of insulin-like growth factor 1 receptor (IGF1R), and increased the expressions of PI3K and Akt, however, these alterations were blocked by miR-378a-3p inhibitor.

Conclusions: miR-378a-3p might participate in the protective effect of SAL in I/R-induced myocardial apoptosis via the IGF1R/PI3K/AKT signaling pathway.
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http://dx.doi.org/10.1016/j.transproceed.2022.05.017DOI Listing
August 2022

Occurrence, distribution, and ecological risk of psychoactive substances in typical lakes and rivers in Qinghai-Tibet Plateau.

Ecotoxicol Environ Saf 2022 Aug 1;242:113928. Epub 2022 Aug 1.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; Center for Environmental Health Risk Assessment and Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; State Environmental Protection Key Laboratory of Ecological Effect and Risk Assessment of Chemicals, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Electronic address:

The occurrence, distribution, and ecological risks of psychoactive substances (PSs) in Qinghai-Tibet Plateau (QTP) was investigated in this study. The surface water samples were collected in July in 2020 from five major water bodies, and 9 PSs were determined by liquid chromatography-mass spectrometry. The mean concentrations of the total PSs were 2.19-96.86 ng/L in lakes and 4.56-34.47 ng/L in rivers. Amphetamine (AMP) was the predominant contaminant both in lakes and rivers with a mean concentration of 12.21 ± 22.76 ng/L and 9.83 ± 6.14 ng/L, respectively. The compositions of PSs in lakes and rivers were significantly different. AMP, methadone (MTD), 3,4-methylenedioxyamphetamine (MDA), and ketamine (KET) were the most detected contaminants in lakes, while in rivers AMP, MDA, heroin (HER), and methamphetamine (METH) were the most detected ones. Concentrations of AMP and MTD, the two predominant drugs, varied spatially, with the decreasing concentration of AMP in the order of Huangshui River > Yamzhog Yumco Lake > Qinghai Lake > Lhasa River > Namco Lake, and of MTD in the order of Qinghai Lake > Namco Lake > Huangshui River > Yamzhog Yumco Lake. The risk quotients (RQs) of PSs ranged from 4.44 × 10 to 4.32 × 10, indicating a low risk of PSs in the aquatic ecosystem in QTP. Compared with other research in the world, the contamination of psychoactive substances in the Qinghai-Tibet Plateau was at relatively low levels with low ecological risks.
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http://dx.doi.org/10.1016/j.ecoenv.2022.113928DOI Listing
August 2022

Construction of Diagnosis Model of Moyamoya Disease Based on Convolution Neural Network Algorithm.

Comput Math Methods Med 2022 25;2022:4007925. Epub 2022 Jul 25.

Department of Neurosurgery, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215000, China.

Objective: The convolutional neural network (CNN) was used to improve the accuracy of digital subtraction angiography (DSA) in diagnosing moyamoya disease (MMD), providing a new method for clinical diagnosis of MMD.

Methods: A total of 40 diagnosed with MMD by DSA in the neurosurgery department of our hospital were included. At the same time, 40 age-matched and sex-matched patients were selected as the control group. The 80 included patients were divided into training set ( = 56) and validation set ( = 24). The DSA image was preprocessed, and the CNN was used to extract features from the preprocessed image. The precision and accuracy of the preprocessed image results were evaluated.

Results: There was no significant difference in baseline data between the training set and validation set ( > 0.05). The precision and accuracy of the images before processing were 79.68% and 81.45%, respectively. After image processing, the precision and accuracy of the model are 96.38% and 97.59%, respectively. The area under the curve of the CNN algorithm model was 0.813 (95% CI: 0.718-0.826).

Conclusion: This diagnostic method based on CNN performs well in MMD detection.
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http://dx.doi.org/10.1155/2022/4007925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343212PMC
August 2022

A prospective study of specimen eversion to lateral rectum and valgus resection for low rectal cancer.

Front Surg 2022 18;9:926227. Epub 2022 Jul 18.

Department of Gastrointestinal Surgery, The First Affiliated Yijishan Hospital of Wannan Medical College, Wuhu, China.

Purpose: To investigate the safety and efficacy of a reverse puncture device (RPD) and specimen eversion of the rectum for resection in total laparoscopic proctectomy.

Methods: In a prospective study from August 2019 to March 2021, 40 patients underwent a procedure with an RPD and specimen eversion of the rectum for total laparoscopic low rectal cancer resection, that is natural orifice specimen extraction surgery (NOSES), were included in the NOSES group. Forty patients in the control group underwent conventional laparoscopic radical resection for low rectal cancer and were included in the LAP group. Intraoperative- and postoperative-related indicators, recovery and inflammatory factors, quality of life (QOL) and mental health were compared.

Results: All operations were successfully completed. Compared with the LAP group, the NOSES group showed better short-term outcomes, such as time to eating, postoperative pain, and especially postoperative incision-related complications. At the same time, postoperative inflammatory factor levels, psychological trauma, life-related anxiety and depression scores, and QOL were better in the NOSES group than in the LAP group.

Conclusions: The application of an RPD and specimen eversion of the rectum for total laparoscopic low rectal cancer resection is a technically feasible and safe approach with a short-term curative effect.
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http://dx.doi.org/10.3389/fsurg.2022.926227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339670PMC
July 2022

Multimodal imaging of perivascular epithelioid cell tumors in the pericardium.

J Nucl Cardiol 2022 Aug 1. Epub 2022 Aug 1.

WeiLun PET Center, Department of Nuclear Medicine, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, China.

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http://dx.doi.org/10.1007/s12350-022-03057-7DOI Listing
August 2022

miR-532-3p suppresses proliferation and invasion of ovarian cancer cells via GPNMB/HIF-1α/HK2 axis.

Pathol Res Pract 2022 Jul 19;237:154032. Epub 2022 Jul 19.

Center for Translational Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, PR China; Key Laboratory for Tumor Precision Medicine of Shaanxi Province, Xi'an, Shaanxi 710061, PR China. Electronic address:

Objective: Identifying a new target of miR-532-3p and studying its functional mechanism to explore the detailed anti-tumor mechanism of miR-532-3p in ovarian cancer.

Methods: Biological and molecular methods including real-time quantitative PCR (RT-qPCR), Western blotting, colony formation, in vitro migration and invasion assays, glucose consumption and lactate production assays, RNA interference and tumor xenograft mouse models were used to study the role of miR-532-3p and its target in ovarian cancer. mRNA sequencing, dual-luciferase reporter assay and immunohistochemistry (IHC) were used to identify miR-532-3p target. STRING dataset analysis, qPCR and Western blotting were used to investigate the downstream pathway of the target of miR-532-3p.

Results: Forced expression of miR-532-3p inhibited the proliferation, migration and invasion of ovarian cancer cells in vitro and the tumor growth in nude mice. RNA sequencing found 299 mRNAs were downregulated in miR-532-3p-overexpressed ovarian cancer cells, and bioinformatic analysis indicated Glycoprotein Nonmetastatic Melanoma Protein B (GPNMB), a type I membrane glycoprotein, was the potential target of miR-532-3p. GPNMB was reduced at both RNA and protein levels in miR-532-3p-overexpressed ovarian cancer cells. Dual-luciferase reporter assay determined GPNMB as the target of miR-532-3p. Interference of GPNMB inhibited the proliferation, migration, invasion, glucose consumption and lactate production of ovarian cancer cells. Knocking down of GPNMB reduced the protein level of HIF-1α without affecting HIF-1α mRNA level. Overexpression of GPNMB reversed the antitumor effect of miR-532-3p.

Conclusion: miR-532-3p exerted the anti-cancer effect by targeting GPNMB/ HIF-1α/ HK2 pathway to inhibit aerobic glycolysis in ovarian cancer.
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http://dx.doi.org/10.1016/j.prp.2022.154032DOI Listing
July 2022

Preoperative Neutrophil to Lymphocyte Ratio Predicts Complications After Esophageal Resection That can be Used as Inclusion Criteria for Enhanced Recovery After Surgery.

Front Surg 2022 13;9:897716. Epub 2022 Jul 13.

Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao, China.

Background: The neutrophil to lymphocyte ratio (NLR) has been reported as an indicator for poor prognosis in many cancers including esophageal cancer. However, the relationship between the NLR and postoperative complications after esophageal cancer resection remains unclear. At present, enhanced recovery after surgery (ERAS) lacks inclusion criteria. The aim of this study is to determine whether the preoperative NLR (NLR) can predict complications after esophageal cancer resection, which could represent the criteria for ERAS.

Methods: This was a retrospective study on 171 patients who underwent esophagectomy at Hospital between November 2020 and November 2021(68 patients from Changhai Hospital, 65 patients from Shanghai General Hospital and 38 patients from Affiliated Hospital of Qingdao University). Univariate and multivariate logistic regression analyses were performed to demonstrate that the NLR could predict complications after esophagectomy.

Results: A NLR cutoff value of 2.30 was identified as having the greatest ability to predict complications with a sensitivity of 76% and specificity of 65%. Moreover, the Chi-squared test results showed that the NLR was significantly associated with complications (x= 13.641,  < 0.001), and multivariate logistic regression analysis showed that body mass index (BMI), p stage and NLR were independent variables associated with the development of postoperative complications ( < 0.05).

Conclusion: The NLR can predict complications after esophagectomy, and these predicted complications can represent the criteria for recruiting patients for ERAS.
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http://dx.doi.org/10.3389/fsurg.2022.897716DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326077PMC
July 2022

Promising indicators for monitoring microplastic pollution.

Mar Pollut Bull 2022 Jul 28;182:113952. Epub 2022 Jul 28.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Electronic address:

Microplastic pollution is a global crisis. This requires a better understanding of microplastic abundance, distribution, and accumulation to prevent potential risks in the future. However, it is very difficult to accurately identify all polymers of microplastics due to the limits of present detection technologies, which might result in overestimation and underestimation of microplastic pollution. Moreover, it also doesn't meet the growing demand for regular monitoring. Herein, we propose an alternative approach to regularly monitoring microplastics in all environmental mediums. The results could meet the urgent need for understanding the spatiotemporal pattern of microplastic pollution. Environmental policy-makers can use it to monitor microplastic pollutants and then rapidly evaluate the environmental level of microplastic pollution.
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http://dx.doi.org/10.1016/j.marpolbul.2022.113952DOI Listing
July 2022

Heavy metal pollution in the soil of a riverine basin: distribution, source, and potential hazards.

Environ Monit Assess 2022 Jul 29;194(9):618. Epub 2022 Jul 29.

Department of Environmental and Conservation Sciences, University of Swat, Swat, 19130, Pakistan.

Soil pollution with heavy metals (HMs) has become a world environmental problem. This study focuses on surface soil contamination with Cr, Mn, Co, Ni, Cu, Zn, Cd, Hg, Pb, Fe, and Al, their sources, and potential hazards along the basin of River Swat, Pakistan. The average concentrations (mg/kg) of HMs were the most abundant for Al (24,730.19) followed by Fe (22,419.41) > Mn (386.78) > Zn (57.75) > Cr (38.07) > Ni (32.46) > Cu (23.43) > Pb (19.59) > Co (10.77) > Cd (3.18) > Hg (0.12). The concentrations of Cr and Mn in 5.45% each, Co in 10.90%, Zn in 27.27%, Cu in 36.36%, Ni in 41.81%, and Hg in 92.72% of the total soil samples exceeded their respective background values. The geostatistical approaches determined the distribution patterns of HM pollution along the basin, whereas the statistics of principal component analysis exposed the likely sources of HM contamination in the area. Pollution indices evaluated the overall HM distribution and pollution status in the area. Contamination factor showed a high degree of HM contamination in 82% of the total sampling sites, while the geo-accumulation index designated low to moderate contamination with Cr, Mn, Co, Ni, Cu, Zn, Hg, and Pb, and moderate to extreme contamination with Cd, Fe, and Al. The trend of ecological toxicity showed potential ups and downs along with the sites from low to considerable hazard (< 95 < PEHI < 190), whereas the human carcinogenic hazard was within the USEPA acceptable limits (1 × 10-1 × 10), but the non-carcinogenic hazard was higher than the threshold (HI > 1) for children because they are more exposed than adults.
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http://dx.doi.org/10.1007/s10661-022-10287-2DOI Listing
July 2022

The Catalytic Domain of Neuropathy Target Esterase Influences Lipid Droplet Biogenesis and Lipid Metabolism in Human Neuroblastoma Cells.

Metabolites 2022 Jul 12;12(7). Epub 2022 Jul 12.

Chongqing Key Laboratory of Big Data for Bio-Intelligence, School of Bio-Information, Chongqing University of Posts and Telecommunications, Chongqing 400065, China.

As an endoplasmic reticulum (ER)-anchored phospholipase, neuropathy target esterase (NTE) catalyzes the deacylation of lysophosphatidylcholine (LPC) and phosphatidylcholine (PC). The catalytic domain of NTE (NEST) exhibits comparable activity to NTE and binds to lipid droplets (LD). In the current study, the nucleotide monophosphate (cNMP)-binding domains (CBDs) were firstly demonstrated not to be essential for the ER-targeting of NTE, but to be involved in the normal ER distribution and localization to LD. NEST was associated with LD surface and influenced LD formation in human neuroblastoma cells. Overexpression of NEST enhances triacylglycerol (TG) accumulation upon oleic acid loading. Quantitative targeted lipidomic analysis shows that overexpression of NEST does not alter diacylglycerol levels but reduces free fatty acids content. NEST not only lowered levels of LPC and acyl-LPC, but not PC or alkyl-PC, but also widely altered levels of other lipid metabolites. Qualitative PCR indicates that the increase in levels of TG is due to the expression of 1 gene by NEST overexpression. Thus, NTE may broadly regulate lipid metabolism to play roles in LD biogenesis in cells.
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http://dx.doi.org/10.3390/metabo12070637DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319352PMC
July 2022

A Bibliometric Analysis of Leprosy during 2000-2021 from Web of Science Database.

Int J Environ Res Public Health 2022 Jul 6;19(14). Epub 2022 Jul 6.

Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210009, China.

In recent years, after the essential elimination of leprosy (the prevalence of which is <1/100,000), the trends, research hotpots, and frontiers of leprosy research are not clear. This study provides a detailed overview of leprosy in terms of papers, journal, language, year, citations, h-index, author keywords, institution, and country through bibliometrics. The results are as follows: (1) The publication rate has increased in recent years, and 8892 papers were obtained. Most of the publications are in English, and the subject categories are mainly focused on "Dermatology." The "" published the most significant number of papers on leprosy, followed by "" and "." (2) Leprosy-related research was contributed to by 24,672 authors, and the ten authors with the most significant number of publications were identified. (3) The University of London (including the London School of Hygiene and Tropical Medicine) has the highest h-index, and Fundacao Oswaldo Cruz is the most productive institution. (4) Brazil, India, the United States, the United Kingdom, and the Netherlands are the most productive countries, and the collaborative network reveals that they have established close cooperation with other countries. France has the highest average number of citations. (5) The keyword co-occurrence network identifies five highly relevant clusters representing topical issues in leprosy research (public health, leprosy vaccine, immune mechanisms, treatment, and genomics research). Overall, these results provide valuable insights for scholars, research institutions, and policymakers to better understand developments in the field of leprosy.
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http://dx.doi.org/10.3390/ijerph19148234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324497PMC
July 2022

TP53 and LRP1B Co-Wild Predicts Improved Survival for Patients with LUSC Receiving Anti-PD-L1 Immunotherapy.

Cancers (Basel) 2022 Jul 12;14(14). Epub 2022 Jul 12.

Department of Medical Oncology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730, China.

Immunotherapy brought long-term benefits for partial patients with lung squamous cell carcinoma (LUSC). The predictor of anti-PD-L1 therapy was controversial and limited in LUSC. We aimed to explore novel biomarker for LUSC immunotherapy and the potential mechanism. Five hundred and twenty-five Chinese patients (Geneplus cohort) with LUSC underwent targeted sequencing and were involved to explore the genomic profiling. and were the most frequently recurrent genes and correlated to higher tumor mutational burden (TMB). We observed that LUSC patients with and co-wild (co-wild type) were associated with better survival of anti-PD-L1 therapy compared with mutant or mutant (mutant type) in POPAR/OAK cohort. Copy-number variation (CNV) and whole genome doubling (WGD) data from TCGA LUSC cohort were obtained to assess the CNV events. There were fewer CNV alterations and lower chromosome instability in patients with / co-wild compared with those with / mutant. RNA expression data from the TCGA LUSC cohort were collected to explore the differences in RNA expression and tumor immune microenvironment (TIME) between mutant and co-wild groups. The / co-wild type had a significantly increased proportion of multiple tumor-infiltrating lymphocytes (TILs), including activated CD8 T cell, activated dendritic cell (DC), and effector memory CD8 T cell. Immune-related gene sets including checkpoint, chemokine, immunostimulatory, MHC and receptors were enriched in the co-wild type. In conclusion, / co-wild LUSC conferred an elevated response rate in anti-PD-L1 therapy and improved survival, which was associated with a chromosome-stable phenotype and an activated immune microenvironment.
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http://dx.doi.org/10.3390/cancers14143382DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320428PMC
July 2022

Exploring the relative contribution of flood regimes and climatic factors to Carex phenology in a Yangtze River-connected floodplain wetland.

Sci Total Environ 2022 Jul 23;847:157568. Epub 2022 Jul 23.

Key Laboratory of Agro-ecological Processes in Subtropical Region, The Chinese Academy of Sciences, Changsha 410125, China; Dongting Lake Station for Wetland Ecosystem Research, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China. Electronic address:

Hydrological regimes can combine with climatic factors to affect plant phenology; however, few studies have attempted to quantify their complex influences on plant phenology in floodplain wetlands. We obtained phenological information on Carex vegetation through MODIS normalized difference vegetation index (NDVI) data during 2001-2020, and monthly field investigation during 2011-2020. We then explored how these data were correlated with climatic factors and flood regimes in a Yangtze River-connected floodplain wetland (Dongting Lake, China). Our results showed that warmer temperature tended to advance the start of the pre-flooding growing season (SOS1), with a relative contribution of 76.1 %. Flood rising time strongly contributed to controlling the end of the pre-flooding growing season. Flood recession time and inundation duration were dominant factors determining the start of the post-flooding growing season (SOS2). Earlier flood recession time and shortened inundation duration tended to advance the SOS2. Shortened inundation duration, earlier flood recession time, and lower solar radiation tended to advance the end of the post-flooding growing season. The phenology of Carex distributed at high-elevation areas was more affected by hydrology than that of Carex distributed at low-elevation areas. Thus, climatic factors strongly affect the phenology of Carex during the pre-flooding growing season, whereas flood regimes play a dominant role in determining the phenology in the post-flooding growing season. The different responses of Carex phenology to climatic and flooding factors may provide insights for the conservation and management of floodplain wetlands in Yangtze River because Carex are primary food source and habitat for herbivorous waterfowls.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157568DOI Listing
July 2022

Effects of land use and landscape on the occurrence and distribution of microplastics in soil, China.

Sci Total Environ 2022 Jul 22;847:157598. Epub 2022 Jul 22.

Institute of Quality Standard and Testing Technology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. Electronic address:

Microplastic pollution in the soil environment has gathered widespread attention. However, little is known about the effects of land use and landscape patterns on the occurrence and distribution of microplastics in the soil. Herein, the microplastic pollution in different land uses (facility farmland, traditional farmland, orchard, grassland, and woodland) in Yuanmou County, Yunnan Province was investigated. The results showed that the abundance of microplastic ranged from 50.000 to 3450.0 items·kg, with an average abundance of 595.00 ± 740.00 items·kg and a median of 375.00 items·kg. Microplastics were found to primarily be green-coloured (28.4 %), fragment (72.7 %) in shape, <1 mm (75.7 %) in size, and composed of polypropylene (51.4 %). There were significant differences in the abundance and characteristics of the microplastics between different land use types. Microplastic abundance in facility farmlands, traditional farmlands, and orchard lands was significantly higher than that of grasslands and woodlands (p < 0.05). The main shape of the microplastics in facility farmlands, traditional farmlands, and orchards was fragments and it was fibers in grasslands and woodlands. The median particle size of microplastics in facility farmlands (539.50 μm) and traditional farmlands (574.00 μm), was smaller than those in the orchard (737.60 μm), grasslands (697.60 μm), and woodlands (1239.4 μm). Discrepancies among different land uses may be due to various reasons, such as different pollution sources and the weathering degree of microplastics. The results of the redundancy analysis showed that the microplastic abundance was negatively correlated with the largest patch index. It also showed that microplastic pollution was closely related to human activities. This study provides an important basis for further research on microplastic ecosystem risks that are associated with land use and provides a data basis for formulating effective prevention and control strategies and measures to mitigate soil microplastic pollution.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157598DOI Listing
July 2022

Protocol for establishing a protein-protein interaction network using tandem affinity purification followed by mass spectrometry in mammalian cells.

STAR Protoc 2022 09 19;3(3):101569. Epub 2022 Jul 19.

Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024, China.

Identification of protein interactors is fundamental to understanding their functions. Here, we describe a modified protocol for tandem affinity purification coupled with mass spectrometry (TAP/MS), which includes two-step purification. We detail the S-, 2×FLAG-, and Streptavidin-Binding Peptide (SBP)- tandem tags (SFB-tag) system for protein purification. This protocol can be used to identify protein interactors and establish a high-confidence protein-protein interaction network based on computational models. This is particularly useful for identifying interacting proteins for subsequent functional studies. For complete details on the use and execution of this protocol, please refer to Bian et al. (2021).
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http://dx.doi.org/10.1016/j.xpro.2022.101569DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304681PMC
September 2022

IL-6 secretion of CD4 T cells stimulated by LC3-positive extracellular vesicles in human epithelial ovarian cancer.

Clin Transl Oncol 2022 Jul 23. Epub 2022 Jul 23.

Department of Obstetrics and Gynecology, Zhongda Hospital, Medical School of Southeast University, 87 Dingjiaqiao Rd., Nanjing, 210009, Jiangsu, China.

Background: Ovarian cancer (OC) as the most fatal gynecological malignancy worldwide, with epithelial ovarian cancer (EOC) being the predominant and most lethal form, poses a serious threat to human health. LC3-positive extracellular vesicles (LC3 EVs) promote tumorigenesis by educating CD4 T cells in a murine melanoma model. However, regulation of LC3 EVs in human EOC remains largely unknown.  METHODS: Differential analysis of Rab8a, Hsp90α and Il6 expression was performed using GEPIA2. The number of LC3 EVs and the frequency of Heat shock protein 90α LC3 EVs (HSP90α LC3 EVs) in the ascites of EOC patients were tested by flow cytometry. IL-6, IL-10, IFN-γ, IL-4 and TGF-β were measured by ELISA. CD4 T cells were isolated from peripheral blood of healthy human donors using MACS magnetic bead technology.  RESULTS: Higher Rab8a, Hsp90a and Il6 expression of cancer tissues compared with normal adjacent tissues in OC were found. The level of IL-6 was positively correlated with LC3 EVs number, HSP90α LC3 EVs percentage in the ascites, and ROMA index of the patient. In addition, elevated IL-6 production by CD4 T cells induced by LC3 EVs was observed, which was suppressed by anti-HSP90α or anti-TLR2.  CONCLUSIONS: LC3 EVs level and HSP90α LC3 EVs percentage were associated with elevated IL-6 in the ascites of EOC patients. HSP90α on LC3 EVs from human EOC could stimulate CD4 T cell production of IL-6 via TLR2.
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http://dx.doi.org/10.1007/s12094-022-02883-yDOI Listing
July 2022

"Two-in-one" sulfur and nitrogen co-doped fluorescent silicon nanoparticles: Simultaneous as the fluorescent probe and photocatalyst for in-situ real time visual monitoring and degradation of tetracycline antibiotics.

Sci Total Environ 2022 Jul 19;846:157470. Epub 2022 Jul 19.

Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, PR China. Electronic address:

Detection and removal of contaminants are significant for environmental monitoring and remediation. In the present study, "two-in-one" silicon nanoparticles (SiNPs) were designed and prepared to simultaneously act as the fluorescent probe and degradation catalyst to detect and remove tetracycline (TCs) antibiotics. Thiourea and 3-aminopropyltrimethoxysilane were dopant and silicon source to generate fluorescent sulfur and nitrogen co-doped SiNPs (SN-SiNPs). The blue fluorescence of SN-SiNPs was selectively quenched by TCs due to the inner filter effect, whilst accompanied by the newly appeared yellow-green fluorescence resulting from aggregation induced fluorescence emission effect. Based on this phenomenon, SN-SiNPs can be used as fluorescent colorimetric probes for detection of doxycycline, oxytetracycline and tetracycline with limits of detection of 1.8 μg/L, 3.0 μg/L and 4.2 μg/L, respectively; the semi-quantitation can even be visually achieved by naked eyes. Particularly, SN-SiNPs were capable to catalyze the degradation of the three TCs effectively, achieving the removal rates of doxycycline, oxytetracycline and tetracycline of >90 %, >80 % and > 70 % after 240 min exposure to UV light. The catalytic ability of SN-SiNPs was derived from hydroxyl radical (•OH), superoxide radical (•O) and singlet oxygen (O) produced by SN-SiNPs under UV irradiation. Moreover, integrating the fluorescent probe and photocatalyst together, the proposed SN-SiNPs simultaneously realized catalyzing the degradation of the three TCs and in-situ visually monitoring of the degradation process in real time. This study innovatively proposed an integrated probe for the detection and catalytic degradation of TCs, providing a new "two-in-one" strategy for rapid and simple detection and removal of drug pollutants.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157470DOI Listing
July 2022

Design, synthesis, and biological evaluation of dinuclear bismuth(III) complexes with Isoniazid-derived Schiff bases.

J Inorg Biochem 2022 Jul 16;235:111931. Epub 2022 Jul 16.

Hunan Provincial Key Laboratory of Xiangnan Rare-Precious Metals Compounds and Applications, School of Chemistry and Environmental Science, Xiangnan University, Chenzhou 423000, Hunan Province, China.

Four dinuclear bismuth(III) Schiff-base complexes bearing Schiff-base ligands have been synthesized and structurally characterized by single-crystal X-ray diffraction, elemental analysis, and spectral techniques (FT-IR, NMR and MS). The analytical data reveal the bismuth(III) complexes possess 1:1 metal-ligand ratios. In vitro biological studies have revealed that bismuth(III) complexes displayed much higher antibacterial and antitumor activities than their parent ligands, which involves two gram-negative (S. aureus, B. subtili) and two gram-positive (E. coli, P. aeruginosa) bacteria, and human gastric cancer SNU-16 cells. The power-time curves of S. pombe exposed to tested compounds were detected by bio-microcalorimetry. Some thermokinetic parameters (k, Pt and Q) were derived based on the metabolic power-time curves, and their quantitative relationships with the concentrations (c) were further discussed.
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http://dx.doi.org/10.1016/j.jinorgbio.2022.111931DOI Listing
July 2022

KIF26B Is Overexpressed in Medulloblastoma and Promotes Malignant Progression by Activating the PI3K/AKT Pathway.

Anal Cell Pathol (Amst) 2022 12;2022:2552397. Epub 2022 Jul 12.

Department of Neurosurgery, The First Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang, China.

Medulloblastoma is one of the most common malignant tumors of the central nervous system in children. Although KIF2B was reported as an oncogene in several malignant tumor types, its role in medulloblastoma has not been studied so far. The PCR results of our study showed that KIF26B is highly expressed in medulloblastoma, and its high expression is associated with a high clinical stage. Knockdown the expression of KIF26B could significantly impair the proliferation and migration of medulloblastoma cells. KIF26B promotes the malignant progression of medulloblastoma by affecting the expression of phosphorylation of key proteins in the PI3K/AKT signaling pathway. With the help of 740 Y-P, activating the pi3k signaling pathway can partially rescue the phenotype. Therefore, our experimental results suggest that KIF26B is a potential target for medulloblastoma.
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http://dx.doi.org/10.1155/2022/2552397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296275PMC
July 2022

Whole-Genome Sequencing of a Chlorimuron-Ethyl-Degrading Strain: Chenggangzhangella methanolivorans CHL1 and Its Degrading Enzymes.

Microbiol Spectr 2022 Jul 21:e0182222. Epub 2022 Jul 21.

Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecologygrid.458475.f, Chinese Academy of Sciences, Shenyang, China.

Chlorimuron-ethyl is a commonly used sulfonylurea herbicide, and its long-term residues cause serious environmental problems. Biodegradation of chlorimuron-ethyl is effective and feasible, and many degrading strains have been obtained, but still, the genes and enzymes involved in this degradation are often unclear. In this study, whole-genome sequencing was performed on chlorimuron-ethyl-degrading strain, Chenggangzhangella methanolivorans CHL1. The complete genome of strain CHL1 contains one circular chromosome of 5,542,510 bp and a G+C content of 68.17 mol%. Three genes, , , and , were predicted to be involved in the degradation of chlorimuron-ethyl, and this was confirmed by gene knockout and gene complementation experiments. The three genes were cloned and expressed in Escherichia coli BL21 (DE3) to allow for the evaluation of the catalytic activities of the respective enzymes. The glutathione--transferase (GST) catalyzes the cleavage of the sulfonylurea bridge of chlorimuron-ethyl, and the esterases, PnbA and SulE, both de-esterify it. This study identifies three key functional genes of strain CHL1 that are involved in the degradation of chlorimuron-ethyl and also provides new approaches by which to construct engineered bacteria for the bioremediation of environments polluted with sulfonylurea herbicides. Chlorimuron-ethyl is a commonly used sulfonylurea herbicide, worldwide. However, its residues in soil and water have a potent toxicity toward sensitive crops and other organisms, such as microbes and aquatic algae, and this causes serious problems for the environment. Microbial degradation has been demonstrated to be a feasible and promising strategy by which to eliminate xenobiotics from the environment. Many chlorimuron-ethyl-degrading microorganisms have been reported, but few studies have investigated the genes and enzymes that are involved in the degradation. In this work, two esterase-encoding genes (, ) and a glutathione--transferase-encoding gene () responsible for the detoxification of chlorimuron-ethyl by strain Chenggangzhangella methanolivorans CHL1 were identified, then cloned and expressed in Escherichia coli BL21 (DE3). These key chlorimuron-ethyl-degrading enzymes are candidates for the construction of engineered bacteria to degrade this pesticide and enrich the resources for bioremediating environments polluted with sulfonylurea herbicides.
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http://dx.doi.org/10.1128/spectrum.01822-22DOI Listing
July 2022

PDIA2 Bridges Endoplasmic Reticulum Stress and Metabolic Reprogramming During Malignant Transformation of Chronic Colitis.

Front Oncol 2022 4;12:836087. Epub 2022 Jul 4.

Scientific Research Institute, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Background: Chronic inflammation contributes to approximately 20% of cancers; the underlying mechanisms are still elusive. Here, using an animal model of colitis to colon-cancerous transformation, we demonstrated that endoplasmic reticulum (ER) stress couples with metabolic reprogramming to promote a malignant transformation of chronic inflammation.

Methods: The animal model for chronic colitis to colon-cancerous transformation was established in C57BL/6N mice by azoxymethane (AOM) and dextran sodium sulfate (DSS) treatments. The differential proteins in control and AOM/DSS-treated colon mucosa were determined using proteomic analysis; the kinetics of metabolic modifications were monitored by mitochondrial oxygen flux, extracellular acidification, and targeted metabolomics; the molecule linker between ER stress and metabolic modifications were identified by coimmunoprecipitation, KEGG pathway analysis, and the subcutaneous tumor model using gene-specific knockdown colon cancer cells. Tissue array analysis were used to evaluate the differential protein in cancer and cancer-adjacent tissues.

Results: AOM/DSS treatment induced 38 tumors in 10 mice at the 14th week with the mean tumor size 9.35 ± 3.87 mm, which was significantly decreased to 5.85 ± 0.95 mm by the ER stress inhibitor 4-phenylbutyric acid (4PBA). Seven differential proteins were determined from control (1,067 ± 48) and AOM/DSS-treated mucosa (1,077 ± 59); the level of ER protein PDIA2 (protein disulfide isomerase-associated 2) was increased over 7-fold in response to AOM/DSS treatment. PDIA2 interacted with 420 proteins that were involved in 8 signaling pathways, in particular with 53 proteins in metabolic pathways. PDIA2 translocated from ER to mitochondria and interacted with the components of complexes I and II to inhibit oxophosphorylation but increase glycolysis. Knockdown PDIA2 in colon cancer cells restored the metabolic imbalance and significantly repressed tumor growth in the xenograft animal model. 4PBA therapy inhibited the AOM/DSS-mediated overexpression of PDIA2 and metabolic modifications and suppressed colon cancer growth. In clinic, PDIA2 was overexpressed in colon cancer tissues rather than cancer-adjacent tissues and was related with the late stages and lymph node metastasis of colon cancer.

Conclusions: Persistent ER stress reprograms the metabolism to promote the malignant transformation of chronic colitis; PDIA2 serves as a molecule linker between ER stress and metabolic reprogramming. The inhibition of ER stress restores metabolic homeostasis and attenuates the cancerous transformation of chronic inflammation.
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http://dx.doi.org/10.3389/fonc.2022.836087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9289542PMC
July 2022

Correlations of magnetic resonance imaging classifications with preoperative functions among patients with refractory lateral epicondylitis.

BMC Musculoskelet Disord 2022 Jul 20;23(1):690. Epub 2022 Jul 20.

Sports Medicine Service, Beijing Jishuitan Hospital, No. 31, Xin Jie Kou East Street, Xi Cheng District, Beijing, China.

Background: To evaluate the correlations between three magnetic resonance imaging (MRI) classifications and preoperative function in patients with refractory lateral epicondylitis (LE).

Methods: We retrospectively reviewed patients with refractory LE who underwent arthroscopic treatment. Signal changes in the origin of the extensor carpi radialis brevis (ERCB) were evaluated based on three different MRI classification systems. Spearman's rank correlation analysis was used to analyse the correlation between each MRI classification and the preoperative functional and visual analogue scale (VAS). The lateral collateral ligament complex (LCL) in all patients was evaluated using both MRI and arthroscopy. The Mann-Whitney U test was used for the comparison of preoperative VAS and all functional scores between patients with refractory LE combined with LCL lesions, and those without.

Results: There were 51 patients diagnosed with refractory LE between June 2014 to December 2020, all of whom were included in this study. The patients included 32 women and 19 men with a mean age of 49.1 ± 7.6 years (range, 39-60 years). The average duration of symptoms was 21.1 ± 21.2 months (range, 6-120 months). The intra-observer agreements for Steinborn et al.'s classification were 77.9%, 76.0%, and 76.7%, respectively. The inter-observer reliabilities of the three classifications were 0.734, 0.751, and 0.726, respectively. The average intra-observer agreement for the diagnosis of abnormal LCL signal was 89.9%, with an overall weighted kappa value of 0.904. The false-positive rate was 50%, and the false-negative rate was 48% for LCL evaluation on MRI. Spearman's rank correlation analysis did not find significant correlation between any of the three MRI classifications and preoperative VAS or any functional scores (all P > 0.05). There were no significant differences in the VAS and functional scores between patients with abnormal LCL signals on MRI and those without LCL lesions (all P > 0.05).

Conclusions: Preoperative MRI findings in patients with refractory LE cannot reflect the severity of functional deficiency. Preoperative MRI grading of the origin of the ERCB and preoperative MRI for LCL signal change cannot assist the surgical plan for the treatment of patients with refractory LE.
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http://dx.doi.org/10.1186/s12891-022-05651-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297620PMC
July 2022

Metformin and Gegen Qinlian Decoction boost islet α-cell proliferation of the STZ induced diabetic rats.

BMC Complement Med Ther 2022 Jul 20;22(1):193. Epub 2022 Jul 20.

Medical experiment center, Shaanxi University of Chinese Medicine, Xianyang, 712046, China.

Background: The traditional Chinese medicine Gegen Qinlian Decoction (GQD), as well as metformin, had been reported with anti-diabetic effects in clinical practice.

Objective: To verify whether these two medicines effectively ameliorate hyperglycemia caused by deficiency of islet β-cell mass which occurs in both type 1 and type 2 diabetes.

Methods: SD rats were injected with a single dose of STZ (55 mg/kg) to induce β-cell destruction. The rats were then divided into control, diabetes, GQD and metformin group. GQD and metformin groups were administered with GQD extract or metformin for 6 weeks. The islet α-cell or β-cell mass changes were tested by immunohistochemical and immunofluorescent staining. The potential targets and mechanisms of GQD and metformin on cell proliferation were tested using in silico network pharmacology. Real-time PCR was performed to test the expression of islet cells related genes and targets related genes.

Results: Both GQD and metformin did not significantly reduce the FBG level caused by β-cell mass reduction, but alleviated liver and pancreas histopathology. Both GQD and metformin did not change the insulin positive cell mass but increased α-cell proliferation of the diabetic rats. Gene expression analysis showed that GQD and metformin significantly increased the targets gene cyclin-dependent kinase 4 (Cdk4) and insulin receptor substrate (Irs1) level.

Conclusion: This research indicates that GQD and metformin significantly increased the α-cell proliferation of β-cell deficiency induced diabetic rats by restoring Cdk4 and Irs1 gene expression.
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http://dx.doi.org/10.1186/s12906-022-03674-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301855PMC
July 2022

Exosome-Derived Circ_0094343 Promotes Chemosensitivity of Colorectal Cancer Cells by Regulating Glycolysis via the miR-766-5p/TRIM67 Axis.

Authors:
Chen Li Xu Li

Contrast Media Mol Imaging 2022 8;2022:2878557. Epub 2022 Jul 8.

Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi 710061, China.

Objective: Currently, the role of circ_0094343 (circPTEN) on the chemosensitivity of CRC remains to be clarified. This study aimed to investigate the role and mechanism of exosome-delivered circ_0094343 in the proliferation, glycolysis, and chemosensitivity of colorectal cancer (CRC) cells.

Methods: Real-time quantitative polymerase chain reaction (qRT-PCR) was utilized to detect the expression level of circ_0094343, miR-766-5p, and TRIM67 (Tripartite motif-containing 67) in CRC clinical tissue samples and cells, transmission electron microscopy (TEM) to observe the morphology of exosomes, and nanoparticle tracking analysis (NTA) system to measure the diameter of exosomes. Besides, PKH67 fluorescent labeling was applied for assessing the level of exosome uptake by cells, MTT and cell clone formation assays for detecting cell proliferation and clone formation, respectively, and related kits for checking the glucose consumption, lactate production, and extracellular acidification rate (ECAR) in cells. Dual-luciferase reporter (DLR) gene assay was used for verifying the targeting relationship between circ_0094343 and miR-766-5p, miR-766-5p and TRIM67, RNA immunoprecipitation (RIP) experiment for the interaction between circ_0094343 and miR-766-5p, and Western blot for the protein level of exosome surface antigens (HSP70, CD63) and TRIM67 in cells in exosomes and cell lysates.

Results: circ_0094343 was significantly downregulated in CRC tissues, chemotherapy-resistant CRC tissues, and metastatic CRC tissues. Moreover, exosomes-carried circ_0094343 played an inhibitory role in the proliferation, clone formation and glycolysis of HCT116 cells. Meanwhile, it could also improve the chemosensitivity of HCT116 cells to 5-fluorouracil (5-FU), oxaliplatin (L-OHP), and doxorubicin (Dox). Additionally, circ_0094343 acted as a sponge for miR-766-5p, and miR-766-5p targeted and regulated TRIM67. In CRC tissues, miR-766-5p expression was negatively correlated with TRIM67 expression, while circ_0094343 was positively associated with TRIM67. Further, mechanistic validation also demonstrated that circ_0094343 could inhibit HCT116 cell proliferation, clone formation, glycolysis, and chemotherapy resistance via the miR-766-5p/TRIM67 axis.

Conclusion: circ_0094343 inhibited the proliferation, clone formation and glycolysis of CRC cells and improved their chemosensitivity to various chemotherapeutic drugs via the miR-766-5p/TRIM67 axis. This finding may provide new insights into the treatment of CRC.
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http://dx.doi.org/10.1155/2022/2878557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286929PMC
July 2022

Aetiology and outcomes of prolonged neonatal jaundice in tertiary centres: data from the China Neonatal Genome Project.

Arch Dis Child Fetal Neonatal Ed 2022 Jul 18. Epub 2022 Jul 18.

The Centre for Pediatric Liver Diseases, Children's Hospital of Fudan University,National Children's Medical Center, Shanghai, China.

Objective: To investigate the distribution of aetiologies and outcomes in neonates with prolonged neonatal jaundice.

Design: An observational study.

Setting: Multiple tertiary centres from the China Neonatal Genome Project.

Patients: Term infants with jaundice lasting more than 14 days or preterm infants with jaundice lasting more than 21 days were recruited between 1 June 2016 and 30 June 2020.

Main Outcome Measures: Aetiology and outcomes were recorded from neonates with prolonged unconjugated hyperbilirubinaemia (PUCHB) and prolonged conjugated hyperbilirubinaemia (PCHB).

Results: A total of 939 neonates were enrolled, and known aetiologies were identified in 84.1% of neonates (790 of 939). Among 411 neonates with PCHB, genetic disorders (27.2%, 112 of 411) were the leading aetiologies. There were 8 deceased neonates, 19 neonates with liver failure and 12 with neurodevelopmental delay. Among 528 neonates with PUCHB, a genetic aetiology was identified in 2 of 219 neonates (0.9%) who showed disappearance of jaundice within 4 weeks of age and in 32 of 309 neonates (10.4%) with persistent jaundice after 4 weeks of age. A total of 96 of 181 neonates (53.0%) who received genetic diagnoses had their clinical diagnosis modified as a result of the genetic diagnoses.

Conclusion: Known aetiologies were identified in approximately 80% of neonates in our cohort, and their overall outcomes were favourable. Genetic aetiology should be considered a priority in neonates with PCHB or the persistence of jaundice after 4 weeks of age. Moreover, genetic data can modify the clinical diagnosis and guide disease management, potentially improving outcomes.
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http://dx.doi.org/10.1136/archdischild-2021-323413DOI Listing
July 2022
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