Publications by authors named "Li Xie"

969 Publications

Media Portrayal of Type 1 Diabetes in North American Television and Film.

Can J Diabetes 2022 Apr 27. Epub 2022 Apr 27.

School of Human Nutrition, McGill University, Montréal, Québec, Canada. Electronic address:

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http://dx.doi.org/10.1016/j.jcjd.2022.04.007DOI Listing
April 2022

Bionic Regulators Break the Ecological Niche of Pathogenic Bacteria for Modulating Dysregulated Microbiome in Colitis.

Adv Mater 2022 Aug 4:e2204650. Epub 2022 Aug 4.

J. Yang, G. Zhang, M. Peng, S. Tan, S. Ge, X. Yang,Y. Liang, Z. Wen, L. Xie, T. Zhou, S. Wu, J. An, Y. Wang, K. Zhang, Prof. Z. Zhang, Prof. J. Liu, Prof. J. Shi, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, China.

Therapeutic approaches that reprogram the gut microbiome are promising strategies to alleviate and cure inflammatory bowel disease (IBD). However, abnormal expansion of E. coli during inflammation can promote pathogenic bacteria occupying ecological niches to resist reprogramming of the microbiome. Herein, we develop a bionic regulator (CaWO @YCW) to efficiently and precisely regulate the gut microbiome by specifically suppressing the abnormal expansion of E. coli during colitis and boosting probiotic growth. Inspired by the binding of E. coli strains to the mannose-rich yeast cell wall (YCW), we chose YCW as the bionic shell to encapsulate CaWO . We demonstrate that the YCW shell endows CaWO with superior resistance to the harsh environment of the gastrointestinal tract and adheres to the abnormally expanded E. coli in colitis, specifically as a positioner. Notably, the high expression of calprotectin at the colitis site triggers the release of tungsten ions through calcium deprivation in CaWO , thus inhibiting E. coli growth by replacing molybdenum in the molybdopterin cofactor. Moreover, YCW functions as a prebiotic and promotes probiotic growth. Consequently, CaWO @YCW can efficiently and precisely reprogram the gut microbiome by eliminating pathogenic bacteria and providing prebiotics, resulting in an extraordinary therapeutic advantage for DSS-induced colitis. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202204650DOI Listing
August 2022

Progress in the treatment of drug-induced liver injury with natural products.

Pharmacol Res 2022 Jul 23;183:106361. Epub 2022 Jul 23.

Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, School of Pharmacy, Anhui Medical University, Hefei 230032, China; Anhui Key Lab. of Bioactivity of Natural Products, Institute for Liver Diseases of Anhui Medical University, Anhui Medical University, Hefei 230032, China. Electronic address:

There are numerous prescription drugs and non-prescription drugs that cause drug-induced liver injury (DILI), which is the main cause of liver disease in humans around the globe. Its mechanism becomes clearer as the disease is studied further. For an instance, when acetaminophen (APAP) is taken in excess, it produces N-acetyl-p-benzoquinone imine (NAPQI) that binds to biomacromolecules in the liver causing liver injury. Treatment of DILI with traditional Chinese medicine (TCM) has shown to be effective. For example, activation of the Nrf2 signaling pathway as well as regulation of glutathione (GSH) synthesis, coupling, and excretion are the mechanisms by which ginsenoside Rg1 (Rg1) treats APAP-induced acute liver injury. Nevertheless, reducing the toxicity of TCM in treating DILI is still a problem to be overcome at present and in the future. Accumulated evidences show that hydrogel-based nanocomposite may be an excellent carrier for TCM. Therefore, we reviewed TCM with potential anti-DILI, focusing on the signaling pathway of these drugs' anti-DILI effect, as well as the possibility and prospect of treating DILI by TCM based on hydrogel materials in the future. In conclusion, this review provides new insights to further explore TCM in the treatment of DILI.
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http://dx.doi.org/10.1016/j.phrs.2022.106361DOI Listing
July 2022

Impact of Salt Intake and RAAS blockade on lung SARS CoV-2 Host Factors.

Kidney Blood Press Res 2022 Jul 25. Epub 2022 Jul 25.

Introduction: The angiotensin-converting enzyme 2 (ACE2) as well as the transmembrane protease serine type 2 (TMPRSS2) have been found to play roles in cell entry for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus causing Coronavirus disease 2019 (COVID-19). SARS-CoV-2 infection risk and severity of COVID-19 might be indicated by the expression of ACE2 and TMPRSS2 in the lung.

Methods: A high salt diet rat model and RAAS blockade were used to test whether these factors affect ACE2 and TMPRSS2 expression of the lung. A normal (0.3% NaCl), a medium (2% NaCl), or a high (8% NaCl) salt diet was fed to rats for 12 weeks, along with enalapril or telmisartan, before examining the lung for histopathological alteration. Using immunofluorescence and qRT-PCR, the localization as well as mRNA expression of ACE2 and TMPRSS2 were investigated.

Results: The findings provide evidence that both TMPRSS2 and ACE2 are highly expressed in bronchial epithelial cells as well as ACE2 was also expressed in alveolar type2 (AT2) cells. High salt diet exposure in rats leads to elevated ACE2 expression on protein level. Treatment with RAAS blockers had no effect on lung tissue expression of ACE2 and TMPRSS2.

Conclusions: These findings offer biological support regarding the safety of these drugs that are often prescribed to COVID-19 patients with cardiovascular co-morbidity. High salt intake on the other hand might adversely affect COVID-19 outcome. Our preclinical data should stimulate clinical studies addressing this point of concern.
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http://dx.doi.org/10.1159/000525368DOI Listing
July 2022

Ultraviolet B modulates gamma radiation-induced stress responses in Lemna minor at multiple levels of biological organisation.

Sci Total Environ 2022 Jul 19;846:157457. Epub 2022 Jul 19.

Norwegian Institute for Water Research (NIVA), Section of Ecotoxicology and Risk Assessment, Økernveien 94, N-0349 Oslo, Norway; Norwegian University of Life Sciences (NMBU), Faculty of Environmental Sciences and Natural Resource Management (MINA), N-1432 Ås, Norway; Norwegian University of Life Sciences (NMBU), Centre for Environmental Radioactivity, N-1432 Ås, Norway. Electronic address:

Elevated levels of ionizing and non-ionizing radiation may co-occur and pose cumulative hazards to biota. However, the combined effects and underlying toxicity mechanisms of different types of radiation in aquatic plants remain poorly understood. The present study aims to demonstrate how different combined toxicity prediction approaches can collectively characterise how chronic (7 days) exposure to ultraviolet B (UVB) radiation (0.5 W m) modulates gamma (γ) radiation (14.9, 19.5, 43.6 mGy h) induced stress responses in the macrophyte Lemna minor. A suite of bioassays was applied to quantify stress responses at multiple levels of biological organisation. The combined effects (no-enhancement, additivity, synergism, antagonism) were determined by two-way analysis of variance (2 W-ANOVA) and a modified Independent Action (IA) model. The toxicological responses and the potential causality between stressors were further visualised by a network of toxicity pathways. The results showed that γ-radiation or UVB alone induced oxidative stress and programmed cell death (PCD) as well as impaired oxidative phosphorylation (OXPHOS) and photosystem II (PSII) activity in L. minor. γ-radiation also activated antioxidant responses, DNA damage repair and chlorophyll metabolism, and inhibited growth at higher dose rates (≥20 mGy h). When co-exposed, UVB predominantly caused non-interaction (no-enhancement or additive) effects on γ-radiation-induced antioxidant gene expression, energy quenching in PSII and growth for all dose rates, whereas antagonistic effects were observed for lipid peroxidation, OXPHOS, PCD, oxidative stress, chlorophyll metabolism and genes involved in DNA damage responses. Synergistic effects were observed for changes in photochemical quenching and non-photochemical quenching, and up-regulation of antioxidant enzyme genes (GST) at one or more dose rates, while synergistic reproductive inhibition occurred at all three γ-radiation dose rates. The present study provides mechanistic knowledge, quantitative understanding and novel analytical strategies to decipher combined effects across levels of biological organisation, which should facilitate future cumulative hazard assessments of multiple stressors.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157457DOI Listing
July 2022

Synergistic hydroxyl radical formation, system XC- inhibition and heat shock protein crosslinking tango in ferrotherapy: A prove-of-concept study of "sword and shield" theory.

Mater Today Bio 2022 Dec 7;16:100353. Epub 2022 Jul 7.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Rd, Haidian District, Beijing, 100085, China.

Ferroptosis provide new insights into designing nanomedicines for enhanced cancer therapy; however, its antitumor efficacy is relatively low, mainly due to self-protective mechanism of cancer cells, , heat shock protein (HSP) overexpression. Since HSPs can be modified/inhibited by lipid peroxidation (LPO) ending products, we construct a nanoplatform, namely [email protected], to amplify intracellular reactive oxygen species (ROS) and LPO for synergistic ferrotherapy. Upon tumor acidic microenvironment and local near-infrared stimuli, this nanoplatform releases FeO and reacts with intracellular hydrogen peroxide (HO) to promote Fenton reaction, and yields significant intracellular ROS (specifically hydroxyl radical, OH) and LPO. In turn, LPO ending products crosslink HSPs to destroy self-preservation pathways of cancer cells to enhance anticancer effect. Meanwhile, the released erastin inhibits system X signal pathway to depletes glutathione. FeO loading further provides magnetic resonance imaging T2-weighted signal to guide anti-tumor treatment. Together, this nanoplatform not only provides OH (as a "sword" to attack tumor cells), but also inhibits system X signal pathway and crosslinks HSP (break down the "shield" of tumor cells) to maximize synergistic ferro-therapeutic effect. [email protected] plus laser irradiation possessed highly efficient tumor suppression with magnified the levels of OH and inactive glutathione peroxidase 4 (GPX4), which can promote the development of precise cooperative cancer therapy.
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http://dx.doi.org/10.1016/j.mtbio.2022.100353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294558PMC
December 2022

Deciphering the internal driving mechanism of microbial community for carbon conversion and nitrogen fixation during food waste composting with multifunctional microbial inoculation.

Bioresour Technol 2022 Jul 15:127623. Epub 2022 Jul 15.

Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, PR China; Shanghai Institute of Pollution Control and Ecological Security, 1239 Siping Road, Shanghai 200092, PR China. Electronic address:

In this study, the effects of multifunctional microbial inoculation on food waste composting based on the synergistic property between organic matter degradation and nitrogen fixation were investigated. The results showed that inoculation simultaneously strengthened organic matter degradation by 9.9% and improved the nitrogen content by 20.6% compared with that of the control group. Additionally, spectral analysis demonstrated that inoculation was conducive to the enhanced humification, which was supported by the improvement in polyphenol oxidase activity. Microbial analysis showed that most of the introduced microorganisms (Bacillus, Streptomyces, Saccharomonospora) successfully colonized, and stimulated the growth of other indigenous microorganisms (Enterobacter, Paenibacillus). Meanwhile, the change in microbial community structure was accompanied by the enhanced tricarboxylic acid cycle and amino acid metabolism. Furthermore, network analysis and structural equation model revealed that the enhanced cooperation of microorganisms, in which more carbon sources could be provided by cellulose decomposition for nitrogen fixation.
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http://dx.doi.org/10.1016/j.biortech.2022.127623DOI Listing
July 2022

Photoswitchable CRISPR/Cas12a-Amplified and [email protected] Nanoemitter Based Triple-Amplified Diagnostic Electrochemiluminescence Biosensor for Detection of miRNA-141.

ACS Appl Mater Interfaces 2022 Jul 15. Epub 2022 Jul 15.

School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China.

In this work, a CRISPR/Cas12a initiated switchable ternary electrochemiluminescence (ECL) biosensor combined with a [email protected] nanoemitter is presented for the in vitro monitoring of miRNA-141. Benefiting from the advantages of high-throughput cargo payload capability and superconductivity, three-dimensional reduced graphene oxide (3D-rGO) was designated as an introductory conducting stratum of a paper working electrode (PWE). With the collaborative participation of [email protected] NPs, the transmutation of TPrA in the Ru(bpy)/TPrA system can be riotously expedited into exorbitant free radical ions TPrA, which provoked the exaggeration of the ECL signal. Moreover, the programmable enzyme-free hybrid chain reaction (HCR) amplifier on the PWE surface accurately anchored the assembly of nucleic acid tandem and accomplished the secondary recursion of the signal. Impressively, the multifunctional CRISPR/Cas12a with nonspecific cis/trans-splitting decomposition manipulated the photoswitch of the "on-off" signal state that avoided the false-positive diagnosis. The presented multistrategy cooperative biosensor demonstrated extraordinary sensitivity and specificity, with a low detection limit of 3.3 fM (S/N = 3) in the concentration scope from 10 fM to 100 nM, which fully corresponded to the expectation. Overall, this innovative methodology paved a generous avenue for evaluating multifarious biotransformations and provided a tremendous impetus to the development of real-time diagnosis and clinical detection of other biomarkers.
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http://dx.doi.org/10.1021/acsami.2c08823DOI Listing
July 2022

Liensinine Inhibits Cell Growth and Blocks Autophagic Flux in Nonsmall-Cell Lung Cancer.

J Oncol 2022 1;2022:1533779. Epub 2022 Jul 1.

Department of Clinical Laboratory, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China.

Liensinine is a bioactive component of Plumula Nelumbinis extracted from the green embryo of the mature seeds of Nelumbonaceae and exhibits therapeutic functions and noteworthy anti-tumor effects in recent studies. However, the potential anti-tumor property and the underlying mechanisms of liensinine in nonsmall-cell lung cancer (NSCLC) have not been illustrated. In this study, we demonstrated that liensinine has the potential anti-tumor property, and it could inhibit growth of NSCLC and . In addition, we found that although it induced significant accumulation of autophagosomes, liensinine could quench them for degradation and blocked autophagic flux. Importantly, we observed that liensinine inhibited the normal function of mitochondrial energy supply and impaired the lysosomal function. This research firstly provides a possibility insight that liensinine could be a novel therapeutic strategy for NSCLC.
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http://dx.doi.org/10.1155/2022/1533779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270144PMC
July 2022

SIRT6 regulates obesity-induced oxidative stress via ENDOG/SOD2 signaling in the heart.

Cell Biol Toxicol 2022 Jul 7. Epub 2022 Jul 7.

School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210006, China.

The sirtuin 6 (SIRT6) participates in regulating glucose and lipid homeostasis. However, the function of SIRT6 in the process of cardiac pathogenesis caused by obesity-associated lipotoxicity remains to be unveiled. This study was designed to elucidate the role of SIRT6 in the pathogenesis of cardiac injury due to nutrition overload-induced obesity and explore the downstream signaling pathways affecting oxidative stress in the heart. In this study, we used Sirt6 cardiac-specific knockout murine models treated with a high-fat diet (HFD) feeding to explore the function and mechanism of SIRT6 in the heart tissue during HFD-induced obesity. We also took advantage of neonatal cardiomyocytes to study the role and downstream molecules of SIRT6 during HFD-induced injury in vitro, in which intracellular oxidative stress and mitochondrial content were assessed. We observed that during HFD-induced obesity, Sirt6 loss-of-function aggravated cardiac injury including left ventricular hypertrophy and lipid accumulation. Our results evidenced that upon increased fatty acid uptake, SIRT6 positively regulated the expression of endonuclease G (ENDOG), which is a mitochondrial-resident molecule that plays an important role in mitochondrial biogenesis and redox homeostasis. Our results also showed that SIRT6 positively regulated superoxide dismutase 2 (SOD2) expression post-transcriptionally via ENDOG. Our study gives a new sight into SIRT6 beneficial role in mitochondrial biogenesis of cardiomyocytes. Our data also show that SIRT6 is required to reduce intracellular oxidative stress in the heart triggered by high-fat diet-induced obesity, involving the control of ENDOG/SOD2.
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http://dx.doi.org/10.1007/s10565-022-09735-zDOI Listing
July 2022

Role of interspecies electron transfer stimulation in enhancing anaerobic digestion under ammonia stress: Mechanisms, advances, and perspectives.

Bioresour Technol 2022 Jun 30;360:127558. Epub 2022 Jun 30.

Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, PR China; Shanghai Institute of Pollution Control and Ecological Security, 1239 Siping Road, Shanghai 200092, PR China. Electronic address:

Ammonia stress is a commonly encountered issue in anaerobic digestion (AD) process when treating proteinaceous substrates. The enhanced relationship between syntrophic bacteria and methanogens triggered by interspecies electron transfer (IET) stimulation is one of the potential mechanisms for an improved methane yield from the AD plant under ammonia-stressed condition. There is, however, lack of synthesized information on the mechanistic understanding of IET facilitation in the ammonia-stressed AD processes. This review critically discusses recovery of AD system from ammonia-stressed condition, focusing on H transfer, redox compound-mediated IET, and conductive material-induced direct IET. The effects and the associated mechanisms of IET stimulation on mitigating ammonia stress and promoting methanogenesis were elucidated. Finally, prospects and challenges of IET stimulation were critically discussed. This review highlights, for the first time, the critical role of IET stimulation in enhancing AD process under ammonia-stressed condition.
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http://dx.doi.org/10.1016/j.biortech.2022.127558DOI Listing
June 2022

Quantification of Tumor Abnormal Proteins in the Diagnosis and Postoperative Prognostic Evaluation of Gastric Cancer.

Clin Med Insights Oncol 2022 24;16:11795549221104440. Epub 2022 Jun 24.

Department of General Surgery, The First Affiliated Hospital, Nanjing Medical University, Nanjing, China.

Background: Abnormal glycosylation of proteins has been identified in almost all types of cancers and is closely related to the cancer progression, metastasis, and survival of cancer patients. This study was to explore the values of serum tumor abnormal protein (TAP), an abnormal glycochain protein, in the diagnosis and prognosis of gastric cancer (GC).

Methods: A total of 335 GC patients were included as the study group, and another 335 subjects served as the control group. Tumor abnormal protein expression was compared between the 2 groups. Correlation analysis was used to assess the correlations of TAP with clinicopathological factors. Gastric cancer patients were divided into training set and test set at a ratio of 2:1. Univariate and multivariate Cox regression analyses in training set were used to evaluate the prognostic significance of TAP in GC patients and explore the independent risk factors for overall survival (OS) and disease-free survival (DFS) to establish a prognostic model, followed by testing of the model. According to the median of TAP, 335 GC patients were divided into 2 groups to plot the survival curves of OS and DFS.

Results: Tumor abnormal protein expression in the study group was significantly higher than in the control group. Taking the best cut-off value of TAP (110.128 μm) as the diagnostic criteria for GC, the sensitivity and specificity of TAP were 83.58% and 97.61%, respectively, and the area under the receiver operating characteristics (ROC) curve was 0.935, which was not inferior to computed tomography (CT). Tumor abnormal protein expression was an independent risk factor for OS and DFS. The prognostic predictive value of TAP was better than that of pathological stage in GC patients. The model with TAP was effective in predicting prognosis.

Conclusion: Tumor abnormal protein is an effective indicator for early screening and prognostic evaluation of GC and can also assist the clinical diagnosis and treatment of GC.
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http://dx.doi.org/10.1177/11795549221104440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237931PMC
June 2022

Teenage Childbearing as an Independent Risk Factor for Stress Urinary Incontinence in American Women.

Urol J 2022 Jun 26. Epub 2022 Jun 26.

Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Purpose: To evaluate the associations among teenage childbearing (Age at first birth<=19 years old) with later-life risk of stress and urgency urinary incontinence (SUI, UUI) in American women using nationally representative data from America.

Materials And Methods: Data from the National Health and Nutrition Examination Survey (NHANES) from 2015 to 2018 were merged to include 2673 women. The question, "How old were you at the time of your first live birth?" was used to assess teenage childbearing. Urinary incontinence was ascertained by self-report. Multivariable logistic regression models were used to assess the association between teenage childbearing and urinary incontinence in American women, controlling for potential confounders.

Results: Among the 2673 women with complete data, the prevalence of SUI was 27.3%, and the prevalence of UUI was 22.1%. Overall, 856 of female had given birth at or before the age of nineteen. Teenage childbearing was significantly associated with SUI (OR=1.9, 95%CI=1.5-2.3, p<0.001), but teenage childbearing was not associated with UUI (OR=1.2, 95%CI=1.0-1.5, p=0.0658).

Conclusion: After controlling for known risk factors, teenage childbearing seems to be significantly related to female stress urinary incontinence.
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http://dx.doi.org/10.22037/uj.v19i.7223DOI Listing
June 2022

Evaluation of the predictive values of elevated serum L-homoarginine and dimethylarginines in preeclampsia.

Amino Acids 2022 Jun 26. Epub 2022 Jun 26.

Department of Laboratory Medicine, Wusong Branch, Zhongshan Hospital, Fudan University, Shanghai, 200940, China.

L-homoarginine (hARG) is involved in nitric oxide biosynthesis, but its role and concentration in preeclampsia (PE) have not been fully revealed. The purpose of this study was to develop and validate a feasible clinical assay to quantify serum hARG, arginine (ARG), asymmetric (ADMA) and symmetric dimethylarginines (SDMA) levels by LC-MS/MS and investigate their differences at different stages of pregnancy with or without preeclampsia. Serum samples were collected from 84 pregnant women without complications (controls), 84 with mild preeclampsia (MPE), and 81 with severe preeclampsia (SPE) at various gestation stages (before the 20th week, during the 20th-28th week or after the 28th week of gestation). No significant difference in ARG levels was observed between PE and controls at any stage (P > 0.05). The serum hARG levels and hARG/ADMA ratios of MPE before the 20th week were higher than those of controls (P < 0.001). ADMA levels of MPE were higher than those of controls during the 20th-28th week (P < 0.01). SDMA levels of SPE were higher than those of MPE (P < 0.01) and controls (P < 0.05) after the 28th week. Elevated serum hARG before the 20th week was identified as an independent predictor for PE (OR = 1.478, 95% CI 1.120-1.950). ROC curve analysis showed serum hARG before the 20th week had a good potential to predict MPE (AUC = 0.875, 95% CI 0.759-0.948). In conclusion, our study indicated that elevated serum hARG and dimethylarginine levels detected by LC-MS/MS might serve as potential biomarkers for the early prediction of PE.
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http://dx.doi.org/10.1007/s00726-022-03177-xDOI Listing
June 2022

Identification and Characterization of Two Novel Noda-like Viruses from Rice Plants Showing the Dwarfing Symptom.

Viruses 2022 05 27;14(6). Epub 2022 May 27.

State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.

Nodaviruses are small bipartite RNA viruses and are considered animal viruses. Here, we identified two novel noda-like viruses (referred to as rice-associated noda-like virus 1 (RNLV1) and rice-associated noda-like virus 2 (RNLV2)) in field-collected rice plants showing a dwarfing phenotype through RNA-seq. RNLV1 genome consists of 3335 nt RNA1 and 1769 nt RNA2, and RNLV2 genome consists of 3279 nt RNA1 and 1525 nt RNA2. Three conserved ORFs were identified in each genome of the two novel viruses, encoding an RNA-dependent RNA polymerase, an RNA silencing suppressor, and a capsid protein, respectively. The results of sequence alignment, protein domain prediction, and evolutionary analysis indicate that these two novel viruses are clearly different from the known nodaviruses, especially the CPs. We have also determined that the B2 protein encoded by the two new noda-like viruses can suppress RNA silencing in plants. Two reverse genetic systems were constructed and used to show that RNLV1 RNA1 can replicate in plant cells and RNLV1 can replicate in insect Sf9 cells. We have also found two unusual peptidase family A21 domains in the RNLV1 CP, and RNLV1 CP can self-cleave in acidic environments. These findings provide new knowledge of novel nodaviruses.
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http://dx.doi.org/10.3390/v14061159DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9231309PMC
May 2022

Recent Progress on Therapeutic Vaccines for Breast Cancer.

Front Oncol 2022 6;12:905832. Epub 2022 Jun 6.

The Comprehensive Cancer Centre of Drum Tower Hospital, Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University, Nanjing, China.

Breast cancer remains the most frequently diagnosed malignancy worldwide. Advanced breast cancer is still an incurable disease mainly because of its heterogeneity and limited immunogenicity. The great success of cancer immunotherapy is paving the way for a new era in cancer treatment, and therapeutic cancer vaccination is an area of interest. Vaccine targets include tumor-associated antigens and tumor-specific antigens. Immune responses differ in different vaccine delivery platforms. Next-generation sequencing technologies and computational analysis have recently made personalized vaccination possible. However, only a few cases benefiting from neoantigen-based treatment have been reported in breast cancer, and more attention has been given to overexpressed antigen-based treatment, especially human epidermal growth factor 2-derived peptide vaccines. Here, we discuss recent advancements in therapeutic vaccines for breast cancer and highlight near-term opportunities for moving forward.
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http://dx.doi.org/10.3389/fonc.2022.905832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207208PMC
June 2022

Exploiting Unreduced Gametes for Improving Ornamental Plants.

Front Plant Sci 2022 6;13:883470. Epub 2022 Jun 6.

College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China.

The formation of gametes with somatic chromosome number or unreduced gametes (2 gametes) is an important process involved in the origin of polyploid plants in nature. Unreduced gametes are the result of meiotic mutations occurring during micro- and mega-sporogenesis. 2 gametes have been identified or artificially induced in a large number of plant species. Breeding of plants through 2 gametes can be advantageous because it combines genetic effects of polyploidy with meiotic recombination and sexual hybridization to produce tremendous genetic variation and heterosis. 2 gametes also occur in ornamental plants, but the potential of using 2 gametes in ornamental plant breeding has not been extensively exploited. Ornamental plants are primarily produced for their esthetic appearance and novelty, not for food and yield, and they can be readily propagated through vegetative means. Triploids, tetraploids, and plants with even higher ploidy levels produced through 2 gametes can be propagated through tissue culture to fix their phenotypes, thus leading to the development of new cultivars. In this review article, we intend to discuss the mechanisms underlying the formation of 2 gametes, techniques for 2 gamete identification, methods for enhancing 2 gamete formation, and the current status in the use of 2 gametes for development of novel ornamental plants. We believe that polyploidy breeding through 2 gametes represents a viable way of developing new cultivars, new species, and even new genera of ornamental plants.
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http://dx.doi.org/10.3389/fpls.2022.883470DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207335PMC
June 2022

The MicroClimate Screen - A microscale climate exposure system for assessing the effect of CO, temperature and UV on marine microalgae.

Mar Environ Res 2022 Jun 16;179:105670. Epub 2022 Jun 16.

Norwegian Institute for Water Research (NIVA), Økernveien 94, N-0579, OSLO, Norway; Norwegian University of Life Sciences (NMBU), Centre for Environmental Radioactivity, Post box 5003, N-1432, Ås, Norway; Norwegian University of Life Sciences (NMBU), Faculty of Environmental Sciences and Natural Resource Management, Post box 5003, N-1432, Ås, Norway. Electronic address:

Global warming and anthropogenic activities are changing the ocean, inducing profound impacts on marine life and ecosystems from changing physical and chemical factors in and above the water column. Rising surface temperatures, ocean acidification, and seasonal variations in UV radiation (UVR), modulated by water clarity and sea-ice extent, affect life cycles of the marine food-web, and directly or indirectly also the global carbon fixation. Diatoms, pelagic microalgae that are responsible for 40% of the marine productivity, have limited capability to avoid exposure to changing ocean conditions, and hence, highly relevant for model studies of the influence of climate change on growth and productivity in the marine environment. A plate-based high-throughput exposure system was constructed to assess the biological effects from relevant climate change factors on the diatom Skeletonema pseudocostatum, conducted as a chronic toxicity tests over 72 h periods. The exposure system consisted of a micro-climate unit and a light-exposure unit, enabling accurate regulation of pCO, temperature, UVR and photosynthetic active radiation (PAR). Changes in physical factors, including pH, dissolved inorganic carbon (DIC), total alkalinity (TA), temperature and salinity in the medium, as well as reduction in growth were characterised to demonstrate performance of the micro exposure system. The results demonstrate that the exposure system successfully simulated ocean acidification and could maintain stable temperature (CV < 3%), PAR and UVR irradiance (CV < 8%). Growth inhibition responses were typically dose-dependent and verified that the micro-exposure system could be used to assess effects and adaptions to climate-relevant stressors.
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http://dx.doi.org/10.1016/j.marenvres.2022.105670DOI Listing
June 2022

Active control of dielectric nanoparticle optical resonance through electrical charging.

Sci Rep 2022 Jun 16;12(1):10117. Epub 2022 Jun 16.

College of Civil Engineering and Mechanics, Lanzhou University, Lanzhou, 730000, China.

A novel method for active control of resonance position of dielectric nanoparticles by increasing the excess charges carried by the nanoparticles is proposed in this paper. We show that as the excess charges carried by the particle increase, the oscillation frequency of excess charges will gradually increase, when it is equal to the incident frequency, resonance occurs due to resonant excitation of the excess charges. What is more, the formula of charges carried by an individual particle required to excite the resonance at any wavelength position is proposed. The resonance position can be directly controlled by means of particle charging, and the enhancement of resonance intensity is more obvious. This work has opened new avenues for the active control of plasmon resonances, which shows great promise for realizing tunable optical properties of dielectric nanoparticles.
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http://dx.doi.org/10.1038/s41598-022-13251-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203548PMC
June 2022

Diagnostic Performance of Metagenomic Next-Generation Sequencing in Pediatric Patients: A Retrospective Study in a Large Children's Medical Center.

Clin Chem 2022 Jul;68(8):1031-1041

Pediatric Translational Medicine Institute, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: Metagenomic next-generation sequencing (mNGS) has the potential to become a complementary, if not essential, test in some clinical settings. However, the clinical application of mNGS in a large population of children with various types of infectious diseases (IDs) has not been previously evaluated.

Methods: From April 2019 to April 2021, 640 samples were collected at a single pediatric hospital and classified as ID [479 (74.8%)], non-ID [NID; 156 (24.4%)], and unknown cases [5 (0.8%)], according to the final clinical diagnosis. We compared the diagnostic performance in pathogen detection between mNGS and standard reference tests.

Results: According to final clinical diagnosis, the sensitivity and specificity of mNGS were 75.0% (95% CI: 70.8%-79.2%) and 59.0% (95% CI: 51.3%-66.7%), respectively. For distinguishing ID from NID, the sensitivity of mNGS was approximately 45.0% higher than that of standard tests (75.0% vs 30.0%; P < 0.001). For fungal detection, mNGS showed positive results in 93.0% of cases, compared to 43.7% for standard tests (P < 0.001). Diagnostic information was increased in respiratory system samples through the addition of meta-transcriptomic sequencing. Further analysis also showed that the read counts in sequencing data were highly correlated with clinical diagnosis, regardless of whether infection was by single or multiple pathogens (Kendall's tau b = 0.484, P < 0.001).

Conclusions: For pediatric patients in critical condition with suspected infection, mNGS tests can provide valuable diagnostic information to resolve negative or inconclusive routine test results, differentiate ID from NID cases, and facilitate accurate and effective clinical therapeutic decision-making.
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http://dx.doi.org/10.1093/clinchem/hvac067DOI Listing
July 2022

The microbial diversity in industrial effluents makes high-throughput sequencing-based source tracking of the effluents possible.

Environ Res 2022 Sep 7;212(Pt E):113640. Epub 2022 Jun 7.

BGI-Shenzhen, Shenzhen, Guangdong 518083, China; BGI-Beijing, Beijing 100101, China. Electronic address:

In order to explore the microbial diversity in industrial effluents, and on this basis, to verify the feasibility of tracking industrial effluents in sewer networks based on sequencing data, we collected 28 sewage samples from the industrial effluents relative to four factories in Shenzhen, China, and sequenced the 16S rRNA genes to profile the microbial compositions. We identified 5413 operational taxonomic units (OTUs) in total, and found that microbial compositions were highly diverse among samples from different locations in the sewer system, with only 107 OTUs shared by 90% of the samples. These shared OTUs were enriched in the phylum of Proteobacteria, the families of Comamonadaceae and Pseudomonadaceae, as well as the genus of Pseudomonas, with both degradation related and pathogenic bacteria. More importantly, we found differences in microbial composition among samples relevant to different factories, and identified microbial markers differentiating effluents from these factories, which can be used to track the sources of the effluents. This study improved our understanding of microbial diversity in industrial effluents, proved the feasibility of industrial effluent source tracking based on sequencing data, and provided an alternative technique solution for environmental surveillance and management.
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http://dx.doi.org/10.1016/j.envres.2022.113640DOI Listing
September 2022

Chemogenetic Activation of Astrocytes in the Basolateral Amygdala Contributes to Fear Memory Formation by Modulating the Amygdala-Prefrontal Cortex Communication.

Int J Mol Sci 2022 May 29;23(11). Epub 2022 May 29.

Department of Neuroscience, City University of Hong Kong, Hong Kong 999077, China.

The basolateral amygdala (BLA) is one of the key brain areas involved in aversive learning, especially fear memory formation. Studies of aversive learning in the BLA have largely focused on neuronal function, while the role of BLA astrocytes in aversive learning remains largely unknown. In this study, we manipulated the BLA astrocytes by expressing the Gq-coupled receptor hM3q and discovered that astrocytic Gq modulation during fear conditioning promoted auditorily cued fear memory but did not affect less stressful memory tasks or induce anxiety-like behavior. Moreover, chemogenetic activation of BLA astrocytes during memory retrieval had no effect on fear memory expression. In addition, astrocytic Gq activation increased c-Fos expression in the BLA and the medial prefrontal cortex (mPFC) during fear conditioning, but not in the home cage. Combining these results with retrograde virus tracing, we found that the activity of mPFC-projecting BLA neurons showed significant enhancement after astrocytic Gq activation during fear conditioning. Electrophysiology recordings showed that activating astrocytic Gq in the BLA promoted spike-field coherence and phase locking percentage, not only within the BLA but also between the BLA and the mPFC. Finally, direct chemogenetic activation of mPFC-projecting BLA neurons during fear conditioning enhanced cued fear memory. Taken together, our data suggest that astrocytes in the BLA may contribute to aversive learning by modulating amygdala-mPFC communication.
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http://dx.doi.org/10.3390/ijms23116092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181030PMC
May 2022

The efficacy and safety of intravenous tranexamic acid on blood loss during total ankle replacement: a retrospective study.

Sci Rep 2022 06 9;12(1):9542. Epub 2022 Jun 9.

Department of Orthopaedics, West China Hospital of Sichuan University, No.37 of Guoxue lane, Wuhou District, Chengdu, 610041, Sichuan, China.

Only a few of studies have reported whether Tranexamic acid (TXA) has the same benefits during total ankle replacement as hip and knee replacements. In our study, we hypothesized that TXA was effective in reducing the perioperative blood loss without increasing the risk of symptomatic venous thromboembolism of patients during total ankle replacement. We retrospectively reviewed 71 patients who underwent total ankle replacement at the foot and ankle surgery center of our institution between January 2017 and May 2021. Patients were divided into two groups according to whether they received intravenous tranexamic acid or not. Patients who received intravenous TXA showed significantly lower estimated intraoperative blood loss, hidden blood loss and total blood loss. The early AOFAS score and ROM at the first month follow up of TXA group were better than the NO-TXA group and the incidence of early wound infection and poor healing (< 1 month postoperative) was significant lower than NO-TXA group. Use of TXA was not associated with significant changes in the incidence of postoperative symptomatic venous thromboembolism. We conclude that intravenous TXA can safely and effectively reduce perioperative blood loss and the incidence of early wound infection and poor healing in total ankle replacement, without increasing the risk of symptomatic venous thromboembolism.The application of TXA following total ankle replacement helped improve ankle function and patient quality of life during the early stage.
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http://dx.doi.org/10.1038/s41598-022-13861-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184638PMC
June 2022

Comparison of Global and Regional Compliance-Guided Positive End-Expiratory Pressure Titration on Regional Lung Ventilation in Moderate-to-Severe Pediatric Acute Respiratory Distress Syndrome.

Front Med (Lausanne) 2022 23;9:805680. Epub 2022 May 23.

Department of Critical Care Medicine, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Purpose: To investigate the difference in the positive end-expiratory pressure (PEEP) selected with chest electrical impedance tomography (EIT) and with global dynamic respiratory system compliance (C) in moderate-to-severe pediatric acute respiratory distress syndrome (pARDS).

Methods: Patients with moderate-to-severe pARDS (PaO/FiO < 200 mmHg) were retrospectively included. On the day of pARDS diagnosis, two PEEP levels were determined during the decremental PEEP titration for each individual using the best compliance (PEEP) and EIT-based regional compliance (PEEP) methods. The differences of global and regional compliance (for both gravity-dependent and non-dependent regions) under the two PEEP conditions were compared. In addition, the EIT-based global inhomogeneity index (GI), the center of ventilation (CoV), and standard deviation of regional delayed ventilation (RVD) were also calculated and compared.

Results: A total of 12 children with pARDS (5 with severe and 7 with moderate pARDS) were included. PEEP and PEEP were identical in 6 patients. In others, the differences were only ± 2 cm HO (one PEEP step). There were no statistical differences in global compliance at PEEP and PEEP [28.7 (2.84-33.15) vs. 29.74 (2.84-33.47) ml/cm HO median (IQR), = 0.028 (the significant level after adjusted for multiple comparison was 0.017)]. Furthermore, no differences were found in regional compliances and other EIT-based parameters measuring spatial and temporal ventilation distributions.

Conclusion: Although EIT provided information on ventilation distribution, PEEP selected with the best C might be non-inferior to EIT-guided regional ventilation in moderate-to-severe pARDS. Further study with a large sample size is required to confirm the finding.
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http://dx.doi.org/10.3389/fmed.2022.805680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167956PMC
May 2022

Molecular Detection of Ofloxacin and Kanamycin Resistance in Patients with MDR and Non-MDR TB from Suburban Districts in Hangzhou, China, 2019-2020.

Biomed Environ Sci 2022 05;35(5):468-471

Department of TB Control and Prevention, Hangzhou Center for Disease Control and Prevention, Hangzhou 310021, Zhejiang, China.

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http://dx.doi.org/10.3967/bes2022.064DOI Listing
May 2022

Evaluation of Tissue Stiffness Around Lesions by Sound Touch Shear Wave Elastography in Breast Malignancy Diagnosis.

Ultrasound Med Biol 2022 Aug 4;48(8):1672-1680. Epub 2022 Jun 4.

Department of Ultrasound, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China. Electronic address:

The aim of the study described here was to assess the evaluation of tissue stiffness around lesions by sound touch shear wave elastography (STE) in breast malignancy diagnosis. This was an institutional ethics committee-approved, single-center study. A total of 90 women with breast masses examined with conventional ultrasound and STE were eligible for enrollment from December 2020 to July 2021. The maximum and mean elastic values of masses, E and E, were determined. Shell function was used to measure the maximum and mean elastic values of tissues around masses in annular shells 0.5, 1.0, 1.5 and 2.0 mm wide, recorded as corresponding E and E. All parameters were analyzed and compared with histopathologic results. Receiver operating characteristic curves were constructed to assess diagnostic performance. Logistic regression analysis was conducted to determine the best diagnostic model. Collagen fiber content of tissues around breast lesions was evaluated using Masson staining and ImageJ software. Ninety women with breast masses were included in this study; 50 had benign (mean diameter 15.84 ± 4.39 mm) and 40 had malignant (mean diameter 17.40 ± 5.42 mm) masses. The diagnostic value of E was the highest (area under the curve = 0.930) with a sensitivity of 87.5% and specificity of 88%. According to stepwise logistic regression analysis, E and age were independent predictors of malignancy. E was also found to be highly correlated with the collagen fiber content of tissue in the malignant group (r = 0.877). Tissue stiffness around lesions measured by STE is a useful metric in identifying malignant breast masses by reflecting collagen fiber content, and E performs best.
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http://dx.doi.org/10.1016/j.ultrasmedbio.2022.04.219DOI Listing
August 2022

Dance Performance in New Rural Areas Based on 3D Image Reconstruction Technology.

Authors:
Li Xie

Comput Intell Neurosci 2022 27;2022:7122053. Epub 2022 May 27.

College of Music and Dance, Yulin Normal University, Yulin 537000, Guangxi, China.

Because of the special ecological environment and humanistic atmosphere in new rural areas, excellent regional dance art has been created. Through computer-aided technology, the essence of dance art in rural areas can be reconstructed and displayed. Therefore, based on 3D image reconstruction technology, this paper obtains the dance data of southeast Guangxi and puts forward the dance display scheme of new rural areas. Through acquisition of image information and image matching algorithm, the dance pose is estimated, and the extracted dance sequence is simplified by 3D reconstruction and mapped by texture. In addition, extraction effect of data set, comparison of dance similarity, and user authenticity score were used to test the five types of dance, which provides ideas for the inheritance and development of traditional folk dance culture.
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http://dx.doi.org/10.1155/2022/7122053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167003PMC
June 2022

Identification of key pathways and genes in nasopharyngeal carcinoma based on WGCNA.

Auris Nasus Larynx 2022 May 31. Epub 2022 May 31.

Department of Thoracic Surgery, Fujian Provincial Hospital, Shengli Clinical College of Fujian Medical University, Fuzhou 350001, China.

Objective: We aim to identify the potential genes and signaling pathways associated with the nasopharyngeal carcinoma (NPC) prognosis using Weighted Gene Co-Expression Network Analysis (WGCNA).

Methods: Gene Expression Omnibus (GEO) query was utilized to download two NPC mRNA microarray data. WGCNA was conducted on differentially expressed genes (DEGs) to obtain tumor-associated gene modules. Genes in core modules were intersected with DEGs for gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis. GSE102349 dataset was devoted to identifying prognostic hub genes by survival analysis and the results were confirmed by quantitative polymerase chain reaction (qPCR).

Results: Co-expression networks were built, and we detected 12 gene modules. The Brown module and Magenta module were extremely associated with NPC samples. GO functional analysis and KEGG pathway analysis was carried out to the genes in the Brown and Magenta modules. Our data indicated that DEGs in Brown module and Magenta module were correlated with the biological regulation, metabolic process, reproduction, and cellular proliferation. Twenty-six hub genes were obtained and were considered to be closely related to NPC. GSE102349 dataset was devoted to identifying prognostic hub genes by survival analysis. The expression of IL33, MPP3 and SLC16A7 in GSE102349 dataset was significantly correlated with the progression-free survival (PFS). The results of qPCR indicated a strong correlation between SLC16A7 expression and the overall survival (OS).

Conclusions: WGCNA contributed to the detection of gene modules and identification of hub genes and crucial genes. These crucial genes might be potential targets for pharmaceutic therapies with potential clinical significance.
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http://dx.doi.org/10.1016/j.anl.2022.05.013DOI Listing
May 2022

Aortic Elasticity and Cardiac Function in Fetuses With Aortic Coarctation.

Front Cardiovasc Med 2022 17;9:870683. Epub 2022 May 17.

Department of Ultrasound Diagnosis, The Second Xiangya Hospital, Central South University, Changsha, China.

Objective: The purpose of the study was to observe the elasticity of the ascending aorta (AAo) in normal fetuses and fetuses with coarctation of the aorta (CoA) by M-mode echocardiography.

Methods: This was a prospective clinical study performed on 16 fetuses with CoA and 48 gestational-age matched normal fetuses. The minimum internal diameter in the diastolic phase (Dmin) and the maximum internal diameter in the systolic phase (Dmax) of the AAo were measured by M-mode echocardiography. The aortic strain was calculated using the formula 100 × (-)/). Doppler echocardiography was performed to measure the cardiac function parameters. Correlations between aortic strain and cardiac function were assessed in fetuses with CoA.

Results: The aortic strain of the ascending aorta in the fetuses with CoA was significantly lower than that in normal fetuses (18.12 ± 4.88% vs. 25.22 ± 4.92%, < 0.01). The fetuses with CoA showed significantly higher combined cardiac output than the controls (471.89 ± 93.98 vs. 411.57 ± 46.35 ml/min/kg, < 0.05). Compared with the normal group, the early diastolic velocities (E') and peak systolic velocities (S') of the left side were obviously decreased in the CoA group ( < 0.05), while the left early diastolic velocity ratio (E/E') was significantly increased in the fetuses with CoA ( < 0.01). For the fetuses with CoA, the aortic strain of the AAo was correlated with the left E/E' and S' ( = -0.522 and 0.504, respectively, < 0.05).

Conclusions: The aortic strain of the ascending aorta was significantly decreased in fetuses with CoA in middle-late gestation. The impaired strain of the ascending aorta was correlated with the left ventricle function in the fetuses with CoA. These findings imply that the abnormalities of the intrinsic aortic wall of CoA might develop early .
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http://dx.doi.org/10.3389/fcvm.2022.870683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9152082PMC
May 2022

Changes of immune-related factors in the blood of schizophrenia and bipolar disorder patients receiving monotherapy.

Transl Psychiatry 2022 05 26;12(1):212. Epub 2022 May 26.

Center for Medical Genetics & Hunan Key Laboratory of Medical Genetics, School of Life Sciences, and Department of Psychiatry, The Second Xiangya Hospital, Central South University, Changsha, China.

Schizophrenia (SCZ) and bipolar disorder (BPD) are associated with abnormal expression of immune-related factors (IRFs), which have been proposed as biomarkers of either disease diagnosis (trait markers) or treatment (state markers). However, the state markers have been found to be less reproducible than the trait markers in previous studies. In the current study, we focused on the changes of IRFs in blood of SCZ and BPD patients receiving monotherapy. SCZ (N = 49) and BPD (N = 49) Chinese patients were recruited at acute episode and followed for 9 to 51 days until remission. Blood samples were collected at two state-points, acute state before treatment and remission state after treatment. A total of 41 IRFs in plasma were quantified by the Luminex assay. After adjusting covariates, we found four cytokines or cytokine receptors were significantly increased at remission when compared to acute episode in all the patients, including CD30, BAFF, CCL20, and CXCL10 (Bonferroni corrected p < 0.05). CD30 and BAFF were consistently increased in both SCZ and BPD while the increase of CCL20 was only observed in BPD but not SCZ when analyzing the two disorders separately. CXCL10 change was not significant in either SCZ or BPD alone. The changes of these four factors were correlated with each other, but not with clinical features. CD30 concentration in the BPD acute state was correlated with sleep quality (Spearman's r = 0.365, Bonferroni corrected p < 0.05). Overall, we found that four factors (CD30, BAFF, CCL20, and CXCL10) might be associated with treatment of psychosis.
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http://dx.doi.org/10.1038/s41398-022-01968-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135722PMC
May 2022
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