Publications by authors named "Li Wang"

10,929 Publications

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Nonalcoholic fatty liver disease and cardiovascular diseases: A Mendelian randomization study.

Metabolism 2022 May 23:155220. Epub 2022 May 23.

Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing 100191, China. Electronic address:

Background: Evidence suggests that nonalcoholic fatty liver disease (NAFLD) is associated with cardiovascular diseases (CVDs). However, the results are inconsistent, and the causality remains to be established.

Objective: We aimed to investigate the potential causal relationship between NAFLD and CVDs, including arterial stiffness, coronary artery disease, heart failure, stroke, ischemic stroke and its subtypes using two-sample Mendelian randomization (MR).

Methods: Genetic instruments were used as proxies for NAFLD. Publicly available Summary-level data were obtained from the UK Biobank, the CARDIoGRAMplusC4D Consortium, the MEGASTROKE Consortium, and other consortia. Six complementary MR methods were performed, including inverse variance weighted method (IVW), MR-Egger, weighted median, weighted mode, MR-PRESSO, and MR-RAPS.

Results: NAFLD was significantly associated with arterial stiffness (β = 0.04 [95%CI, 0.02-0.06], P = 5.53E-04). Moreover, the results remained consistent and robust in the sensitivity analysis. As for heart failure, the IVW method suggested that NAFLD was significantly associated with heart failure (OR = 1.08, 95%CI: 1.02-1.14, P = 0.005) in the absence of pleiotropy. However, there were no significant associations of NAFLD with coronary artery disease, stroke, ischemic stroke, or any ischemic stroke subtype.

Conclusion: The MR study supported the causal effect of NAFLD on arterial stiffness. However, the study did not provide enough evidence suggesting the causal associations of NAFLD with heart failure, coronary artery disease, and any stroke subtypes.
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http://dx.doi.org/10.1016/j.metabol.2022.155220DOI Listing
May 2022

Long-Read Sequencing Revealed Extragenic and Intragenic Duplications of Exons 56-61 in in an Asymptomatic Male and a DMD Patient.

Front Genet 2022 9;13:878806. Epub 2022 May 9.

Genetic and Prenatal Diagnosis Center, Department of Obstetrics and Gynecology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Expanded carrier screening (ECS) has become an increasingly common technique to assess the genetic risks of individuals in the prenatal or preconception period. Unexpected variants unrelated to referral are being increasingly detected in asymptomatic individuals through ECS. In this study, we reported an asymptomatic male with duplication of exons 56-61 in the gene through ECS using whole-exome sequencing (WES), which was also detected in a male patient diagnosed with typical Duchenne muscular dystrophy (DMD). Breakpoint analysis was then performed to explore the potential mechanisms of phenotypic differences using long-read sequencing (LRS), PacBio single-molecule real-time (PacBio SMRT) target sequencing, and Sanger sequencing. Complex structural variations (SVs) on chromosome X were identified in the asymptomatic male, which revealed that the duplication occurred outside the gene; whereas, the duplication in the patient with DMD was a tandem repeat. The phenotypic differences between the two men could be explained by the different breakpoint junctions. To the best of our knowledge, this is the first report of a breakpoint analysis of duplication in two men with different phenotypes. Breakpoint analysis is necessary when the clinical phenotypes are inconsistent with genotypes, and it applies to prenatal testing.
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http://dx.doi.org/10.3389/fgene.2022.878806DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125615PMC
May 2022

Aggregation behavior of partially contacted graphene sheets in six-carbon alkanes: all-atom molecular dynamics simulation.

J Mol Model 2022 May 25;28(6):169. Epub 2022 May 25.

School of Physics and Optoelectronic Engineering, Ludong University, Yantai, 264025, China.

Molecular dynamics simulations are used to investigate the aggregation of the cross-contacted and non-cross-contacted graphene sheets in n-hexane, 2,3-dimethylbutane, and cyclohexane solvents. The results show that the main driving force of the graphene aggregation is the interaction between the graphene sheets, and the interaction between solvent molecules also contributes to the aggregation slightly. The initial graphene configurations and the solvent molecule structures both have effects on the graphene aggregation speed. Specifically, the cross-contacted graphene sheets aggregate faster than the non-cross-contacted configuration, since the interaction between the graphene sheets is larger and the direction of this interaction is conducive to pushing away the solvent molecules adsorbed on the graphene surface. The graphene aggregation speed is larger in n-hexane mainly since the mobility of the solvent molecules is higher than the other two solvents, while the interaction between graphenes/solvents has little influence for the systems used in this work. This work provides useful insights into the graphene aggregation in the solvents with different initial graphene configurations and solvent molecule structures.
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http://dx.doi.org/10.1007/s00894-022-05164-1DOI Listing
May 2022

Hydroxysafflor yellow A triggered a fast-to-slow muscle fiber-type conversion regulating FoxO1 in myocytes.

Food Funct 2022 May 25. Epub 2022 May 25.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Hydroxysafflor yellow A (HSYA) is the main bioactive component of safflower and has been reported to have significant health-promoting abilities. However, the regulation of HSYA on different types of skeletal myofibers is largely unknown. Here, experiments found that the water extract of safflower could significantly increase MyHC I, MB and Tnni1 mRNA expression while downregulating MyHC IIb mRNA expression. Furthermore, HSYA triggered fast-to-slow fiber-type switching and increased gene expression related to mitochondrial biosynthesis both and . Autodock analyses proved that FoxO1 is a potential target of HSYA, and qRT-PCR and western blotting further showed that HSYA significantly promoted the activation of the FoxO1 signaling pathway. Additionally, the levels of PGC1α, downstream of FoxO1, also significantly increased after HSYA treatment. Together, our findings suggested that HSYA triggered a fast-to-slow myofiber-type shift through the FoxO1 signaling pathway.
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http://dx.doi.org/10.1039/d1fo03612bDOI Listing
May 2022

Anti-nuclear matrix protein 2+ juvenile dermatomyositis with severe skin ulcer and infection: A case report and literature review.

World J Clin Cases 2022 Apr;10(11):3579-3586

Department of Rheumatology and Immunology; Ministry of Education Key Laboratory of Child Development and Disorders; National Clinical Research Center for Child Health and Disorders (Chongqing); China International Science and Technology Cooperation base of Child Development and Critical Disorders; Children's Hospital of Chongqing Medical University, Chongqing 400014, China.

Background: Juvenile dermatomyositis (JDM) is an idiopathic inflammatory myopathy that occurs in childhood. It is characterized by muscle weakness and a characteristic rash. Previous literature reports have rarely described JDM with severe skin ulcers and infections.

Case Summary: Herein, we describe a case of a 2-year-old female patient who suffered from JDM, whose myositis-specific autoantibodies were positive for anti-nuclear matrix protein 2 antibody, with progressively worsening skin ulcers and severe infections. The patient was treated with glucocorticoids and various immunosuppressants. Nevertheless, further progression of the disease and the combination of primary disease and severe infection in the later period were fatal.

Conclusion: In children, anti-nuclear matrix protein 2+ JDM combined with skin ulcers often indicates severe disease. In such cases, personalized treatment for the primary disease and infection prevention and control are essential.
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http://dx.doi.org/10.12998/wjcc.v10.i11.3579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048553PMC
April 2022

Encouraging specific biomarkers-based therapeutic strategies for hepatocellular carcinoma.

World J Clin Cases 2022 Apr;10(11):3321-3333

Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China.

The prevention, early discovery and effective treatment of patients with hepatocellular carcinoma (HCC) remain a global medical challenge. At present, HCC is still mainly treated by surgery, supplemented by vascular embolization, radio frequency, radiotherapy, chemotherapy and biotherapy. The application of multikinase inhibitor sorafenib, chimeric antigen receptor T cells, or PD-1/PD-L1 inhibitors can prolong the median survival of HCC patients. However, the treatment efficacy is still unsatisfactory due to HCC metastasis and postoperative recurrence. During the process of hepatocyte malignant transformation, HCC tissues can express and secrete many types of specific biomarkers, or oncogenic antigen molecules into blood, for example, alpha-fetoprotein, glypican-3, Wnt3a (one of the key signaling molecules in the Wnt/β-catenin pathway), insulin-like growth factor (IGF)-II or IGF-I receptor, vascular endothelial growth factor, secretory clusterin and so on. In addition, combining immunotherapy with non-coding RNAs might improve anti-cancer efficacy. These biomarkers not only contribute to HCC diagnosis or prognosis, but may also become molecular targets for HCC therapy under developing or clinical trials. This article reviews the progress in emerging biomarkers in basic research or clinical trials for HCC immunotherapy.
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http://dx.doi.org/10.12998/wjcc.v10.i11.3321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048543PMC
April 2022

Efficacy and safety of Mianyi granules (+mianyi+) for reversal of immune nonresponse following antiretroviral therapy of human immunodeficiency virus-1: a randomized, double-blind, multi-center, placebo-controlled trial.

J Tradit Chin Med 2022 Jun;42(3):432-438

Traditional Chinese Medicine Center for Acquired Immune Deficiency Syndrome Prevention and Treatment, China Academy of Chinese Medical Sciences, Beijing 100700, China.

Objective: To investigate whether Mianyi granules (+mianyi+) are effective and safe in reversing immune nonresponse following antiretroviral therapy (ART) in individuals with human immunodeficiency virus (HIV) infection.

Methods: Randomized, double-blind, multi-center, placebo-controlled trial (factorial design) of daily oral Mianyi granules versus placebo for 72 weeks. A total of 361 HIV-positive individuals receiving ART at five Class III Grade I hospitals in China between September 2013 and January 2016 completed the study. The primary endpoints were frequencies of CD3+, CD4+, CD8+, and CD45RA+ cells at seven timepoints over the 72 weeks. Secondary endpoints included viral loads, clinical symptoms, and quality of life at 72 weeks.

Results: A total of 400 participants were enrolled in the study and randomized, of whom 361 completed the study: 189 individuals (140 men and 49 women) in the Mianyi granule group and 172 individuals (135 men and 37 women) in the placebo group. In the intent-to-treat population, CD4+ T cell counts increased from (193 ± 71) cells/mm at baseline to (288 ± 131) cells/mm post-treatment in the Mianyi granule group and from (200 ± 75) cells/mm at baseline to (264 ± 124) cells/mm post-treatment in the placebo group. Patients treated with Mianyi granule had higher increases in CD4+ T cell counts than those treated with placebo ( = 0.045). Reversal of immune nonresponse was defined as a CD4+ T cell increase of more than 100 cells/mm3. After treatment for 72 weeks, Mianyi granule was effective in reversing immune nonresponse in a higher proportion of individuals (20.2%) compared with placebo (9.7%). CD45RA+ cell counts increased from (34 ± 32) cell/mm at baseline to (51 ± 61) cells/mm post-treatment in the Mianyi granule group and from (37 ± 33) cells/mm at baseline to (48 ± 37) cells/mm post-treatment in the placebo group. Mianyi granules were more effective than placebo in increasing CD45RA+ cell counts.

Conclusions: In ART-treated HIV-positive adults with immune nonresponse, treatment with Mianyi granules for 72 weeks was safe and significantly increased CD4+ and CD45RA+ cell counts, thereby promoting immune reconstitution.
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http://dx.doi.org/10.19852/j.cnki.jtcm.2022.03.010DOI Listing
June 2022

Rapid and sensitive detection of Ebola RNA in an unamplified sample based on CRISPR-Cas13a and DNA roller machine.

Biosens Bioelectron 2022 May 18;211:114393. Epub 2022 May 18.

School of Pharmacy, Jiangsu University, Zhenjiang, 212013, PR China. Electronic address:

A fast and simple Cas13a-based assay approach for direct detecting Ebola RNA in unamplified samples is reported. The procedure (named Cas-Roller) is comprised of a 10-min Cas13a-mediated cleavage protocol, followed by a DNA roller running for 30 min. This involves Cas13a collateral cleaving a suitably designed substrate in the presence of Ebola virus RNA sequence, and the cleavage product is used for DNA roller to amplify and generate fluorescent signals. After optimization of the conditions, the assay is able to achieve a limit of detection as low as 291 aM (∼175 copies RNA/μL) along with excellent anti-interfering performance in human serum and blood detection, which is ∼310-fold improved compared with the direct CRISPR assay. The entire workflow can be completed in ∼40 min at 37 °C without any pre-amplification, transcription, or centrifugation steps, thus avoiding the generation of false-negative or positive results. In addition, the downstream roller reaction is independent of the target sequence, this method can be applied to detect any other RNA by merely redesigning the hybridization regions of the crRNA. Overall, this strategy gives a new idea for the construction of simple and accurate Cas13a-based assays for the direct detection of RNA.
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http://dx.doi.org/10.1016/j.bios.2022.114393DOI Listing
May 2022

Efficient classification of Escherichia coli and Shigella using FT-IR spectroscopy and multivariate analysis.

Spectrochim Acta A Mol Biomol Spectrosc 2022 May 11;279:121369. Epub 2022 May 11.

Kweichow Moutai Group, Renhuai, Guizhou 564501, China. Electronic address:

Accurate and effective discrimination of E. coli and Shigella is an important clinical issue, and there are many limitations in traditional methods of analysis. FT-IR shows great potential in the classification of bacteria with high specificity and low cost. In this study, we evaluated the efficiency of this technique when combined with multivariate analysis for rapid classification of E. coli and Shigella, which is difficult using traditional analytical methods. Machine learning and statistical tools were employed in combination with FT-IR to classify 14 E. coli and 9 Shigella strains. The classification accuracies for select E. coli and Shigella strains from blood agar were 0.7826, 0.8696, and 0.9565 at the genus, species, and strain levels, respectively. In addition, we used the FT-IR data of select strains from three different culture media for cross-validation, yielding an accuracy of 0.3681 at the strain level. These results indicate that the bacterial culture conditions have a significant impact on the FT-IR patterns. Based on this, an improved strategy for training an ensemble classifier model considering bacterial culture factors was constructed, resulting in almost perfect separation with an accuracy of 0.9394 for strain-level classification. These results show the potential of FT-IR combined with multivariate analysis for more reliable bacterial classification.
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http://dx.doi.org/10.1016/j.saa.2022.121369DOI Listing
May 2022

Data-Driven Distributed Information-Weighted Consensus Filtering in Discrete-Time Sensor Networks With Switching Topologies.

IEEE Trans Cybern 2022 May 24;PP. Epub 2022 May 24.

This article proposes a data-driven distributed filtering method based on the consensus protocol and information-weighted strategy for discrete-time sensor networks with switching topologies. By introducing a data-driven method, a linear-like state equation is designed by utilizing only the input and output (I/O) data without a controlled object model. In the identification step, data-driven adaptive optimization recursive identification (DD-AORI) is exploited to identify the recurrence of time-varying parameters. It is proved that for discrete-time switching networks, estimation errors of all nodes are ultimately bounded when data-driven distributed information-weighted consensus filtering (DD-DICF) is executed. The algorithm combines with the received neighbors and direct or indirect observations for the target node to produce modified gains, resulting in a novel state estimator containing an information interaction mechanism. Subsequently, convergence analysis is performed on the basis of the Lyapunov equation to guarantee the boundedness of DD-DICF estimate error. Simulations verify the performance of the DD-DICF against the theoretical results as well as in comparison with some existing filtering algorithms.
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http://dx.doi.org/10.1109/TCYB.2022.3166649DOI Listing
May 2022

Green Manufacturing of Flexible Sensors with a Giant Gauge Factor: Bridging Effect of CNT and Electric Field Enhancement at the Percolation Threshold.

ACS Appl Mater Interfaces 2022 May 24. Epub 2022 May 24.

Department of Mechanical and Industrial Engineering, University of Toronto, Toronto ON M5S 3E8, Canada.

Toxic organic solvents are commonly used to disperse nanomaterials in the manufacturing of flexible conductive composites (e.g., graphene-PDMS). The dry-blended method avoids toxic organic solvent usage but leads to poor performance. Here, we proposed an innovative manufacturing method by adapting the traditional dry-blended method, including two key steps: minor CNT bridging and high-frequency electric field enhancement at the percolation threshold of graphene-PDMS. Significant improvement was achieved in the electrical conductivity (1528 times), the giant gauge factor (>8767.54), and the piezoresistive strain range (30 times) over the traditional dry-blended method. Further applications in measurements of culturing rat neonatal cardiomyocytes and mouse hearts proved that the proposed method has great potential for the manufacturing of nontoxic flexible sensors.
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http://dx.doi.org/10.1021/acsami.2c04296DOI Listing
May 2022

Anti-IAV indole-diterpenoids from the marine-derived fungus .

Nat Prod Res 2022 May 24:1-6. Epub 2022 May 24.

Huanghe Science & Technology College, Zhengzhou, P.R. China.

A new indole-diterpenoid, penijanthine E (), and a known analogue (), were obtained from the PDB culture of the marine-derived fungus ZSS-9. The absolute configuration of was elucidated by calculated TDDFT ECD and DP4plus calculations. The absolute configuration of was confirmed by single-crystal X-ray diffraction analysis and TDDFT ECD calculations. Compounds and showed antiviral activity against influenza A virus (IAV) of A/WSN/33(H1N1) and A/PR/8/34(H1N1) strains with IC values ranging from 12.6 to 46.8 μM.
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http://dx.doi.org/10.1080/14786419.2022.2078820DOI Listing
May 2022

Pulmonary Embolism at Extreme High Altitude: A Study of Seven Cases.

High Alt Med Biol 2022 May 23. Epub 2022 May 23.

Department of Respiratory Disease, General Hospital of Xinjiang Military Command, Urumqi, China.

Wu, Jialin, Xiaobo Han, Haiwen Ke, Li Wang, Kun Wang, Jianli Zhang, Jun Tang, Wei Yan, Guangjun Wang, and Peng Jiang. Pulmonary embolism at extreme high altitude: A study of seven cases. XX:000-000, 2022. The incidence of venous thromboembolism (VTE) is high in high-altitude (HA) areas. We analyzed cases of pulmonary embolism (PE) in extreme HA areas to explore the epidemiological characteristics and risk factors of PE in these regions. Seven cases of PE occurring in an extreme HA region were prospectively collected at an HA (3,800 m) hospital from May to November 2020. All patients resided 5,000 m above sea level and were diagnosed with PE using computed tomography pulmonary angiography. Seven patients (24 ± 3.6 years old) had symptom onset at a mean altitude of 5,200 ± 200 m, and the duration spent at HA ranged from 8 to 210 days (99.29 ± 77.31 days). Cough, expectoration, chest tightness, fever, shortness of breath, and chest pain were the most common symptoms. Six of the seven patients were initially diagnosed with pulmonary inflammation, and four were diagnosed with high-altitude pulmonary edema using computed tomography or X-ray. Most patients presented with an increased concentration of inflammatory cells and high initial D-dimer levels. In this study, a retrospective analysis of PE case data in extreme HA areas suggested that PE was underdiagnosed owing to misdiagnosis or masking by HA-associated disease.
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http://dx.doi.org/10.1089/ham.2021.0109DOI Listing
May 2022

Ex Vivo Organoid Model of Adenovirus-Cre Mediated Gene Deletions in Mouse Urothelial Cells.

J Vis Exp 2022 May 5(183). Epub 2022 May 5.

Department of Urology, Roswell Park Comprehensive Cancer Center; Department of Pharmacology and Therapeutics, Roswell Park Comprehensive Cancer Center;

Bladder cancer is an understudied area, particularly in genetically engineered mouse models (GEMMs). Inbred GEMMs with tissue-specific Cre and loxP sites have been the gold standards for conditional or inducible gene targeting. To provide faster and more efficient experimental models, an ex vivo organoid culture system is developed using adenovirus Cre and normal urothelial cells carrying multiple loxP alleles of the tumor suppressors Trp53, Pten, and Rb1. Normal urothelial cells are enzymatically disassociated from four bladders of triple floxed mice (Trp53: Pten: Rb1). The urothelial cells are transduced ex vivo with adenovirus-Cre driven by a CMV promoter (Ad5CMVCre). The transduced bladder organoids are cultured, propagated, and characterized in vitro and in vivo. PCR is used to confirm gene deletions in Trp53, Pten, and Rb1. Immunofluorescence (IF) staining of organoids demonstrates positive expression of urothelial lineage markers (CK5 and p63). The organoids are injected subcutaneously into host mice for tumor expansion and serial passages. The immunohistochemistry (IHC) of xenografts exhibits positive expression of CK7, CK5, and p63 and negative expression of CK8 and Uroplakin 3. In summary, adenovirus-mediated gene deletion from mouse urothelial cells engineered with loxP sites is an efficient method to rapidly test the tumorigenic potential of defined genetic alterations.
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http://dx.doi.org/10.3791/63855DOI Listing
May 2022

FOXO Dictates Initiation of B Cell Development and Myeloid Restriction in Common Lymphoid Progenitors.

Front Immunol 2022 4;13:880668. Epub 2022 May 4.

Center for Hematology and Regenerative Medicine, Karolinska Institutet, Stockholm, Sweden.

The development of B cells relies on an intricate network of transcription factors critical for developmental progression and lineage commitment. In the B cell developmental trajectory, a temporal switch from predominant to expression occurs at the CLP stage. Utilizing VAV-iCre mediated conditional deletion, we found that the loss of FOXO3 impaired B cell development from LMPP down to B cell precursors, while the loss of FOXO1 impaired B cell commitment and resulted in a complete developmental block at the CD25 negative proB cell stage. Strikingly, the combined loss of FOXO1 and FOXO3 resulted in the failure to restrict the myeloid potential of CLPs and the complete loss of the B cell lineage. This is underpinned by the failure to enforce the early B-lineage gene regulatory circuitry upon a predominantly pre-established open chromatin landscape. Altogether, this demonstrates that FOXO3 and FOXO1 cooperatively govern early lineage restriction and initiation of B-lineage commitment in CLPs.
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http://dx.doi.org/10.3389/fimmu.2022.880668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9116193PMC
May 2022

Neural alterations in opioid-exposed infants revealed by edge-centric brain functional networks.

Brain Commun 2022 5;4(3):fcac112. Epub 2022 May 5.

Biomedical Research Imaging Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Prenatal opioid exposure has been linked to adverse effects spanning multiple neurodevelopmental domains, including cognition, motor development, attention, and vision. However, the neural basis of these abnormalities is largely unknown. A total of 49 infants, including 21 opioid-exposed and 28 controls, were enrolled and underwent MRI (43 ± 6 days old) after birth, including resting state functional MRI. Edge-centric functional networks based on dynamic functional connections were constructed, and machine-learning methods were employed to identify neural features distinguishing opioid-exposed infants from unexposed controls. An accuracy of 73.6% (sensitivity 76.25% and specificity 69.33%) was achieved using 10 times 10-fold cross-validation, which substantially outperformed those obtained using conventional static functional connections (accuracy 56.9%). More importantly, we identified that prenatal opioid exposure preferentially affects inter- rather than intra-network dynamic functional connections, particularly with the visual, subcortical, and default mode networks. Consistent results at the brain regional and connection levels were also observed, where the brain regions and connections associated with visual and higher order cognitive functions played pivotal roles in distinguishing opioid-exposed infants from controls. Our findings support the clinical phenotype of infants exposed to opioids in utero and may potentially explain the higher rates of visual and emotional problems observed in this population. Finally, our findings suggested that edge-centric networks could better capture the neural differences between opioid-exposed infants and controls by abstracting the intrinsic co-fluctuation along edges, which may provide a promising tool for future studies focusing on investigating the effects of prenatal opioid exposure on neurodevelopment.
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http://dx.doi.org/10.1093/braincomms/fcac112DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117006PMC
May 2022

Synergistic Fluoride Adsorption by Composite Adsorbents Synthesized From Different Types of Materials-A Review.

Front Chem 2022 4;10:900660. Epub 2022 May 4.

Xi'an Key Laboratory of Solid Waste Recycling and Resource Recovery, Department of Environmental Science and Engineering, School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an, China.

The reduction of fluoride concentrations in water is one of many concerns. Adsorption is the most widely used technology for fluoride removal and the center to development of adsorption technology is the improvement of adsorbents. This review classifies the typical fluoride removal adsorbents into four types: metal oxides/hydroxides, biopolymers, carbon-based, and other adsorbents. The exploitation of new materials and the synthesis of composite materials are two ways of developing new adsorbents. In comparison to the discovery of novel adsorbents for fluoride adsorption, research into the composite synthesis of different types of conventional adsorbents has proliferated in recent years. The traditional adsorbents used the earliest, metal oxides, can act as active centers in a wide range of applications for modifying and compounding with other types of adsorbents. This study emphasizes reviewing the research on fluoride removal by composite adsorbents synthesized from different types of metal-modified materials. Seven factors were compared in terms of material characterization, initial fluoride concentration, adsorbent dose, pH, temperature, reaction time, and maximum adsorption capacity. The modification of composite adsorbents is facile and the synergistic effect of the different types of adsorbents significantly improves fluoride adsorption capacity. Metal composite adsorbents are synthesized by facile coprecipitation, hydrothermal, or impregnation modification methods. The adsorption mechanisms involve electrostatic attraction, ion exchange, complexation, and hydrogen bonding. The fluoride adsorption capacity of composite adsorbents has generally improved, indicating that most modifications are successful and have application prospects. However, to achieve significant breakthroughs in practical applications, numerous issues such as cost, separation/regeneration performance, and safety still need to be considered.
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http://dx.doi.org/10.3389/fchem.2022.900660DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114667PMC
May 2022

Sleep quality and the associated factors among in-hospital nursing assistants in general hospital: A cross-sectional study.

Heliyon 2022 May 11;8(5):e09393. Epub 2022 May 11.

Department of Nursing, Tianjin Hospital, Tianjin 300211, China.

Background: Sleep quality and the associated factors in professional nurses have been extensively investigated. However, as an important part of the workforce in hospitals, sleep quality and influencing factors among in-hospital nursing assistants is rarely investigated.

Aim: This study aimed to assess the sleep quality and the associated factors of in-hospital nursing assistants in general hospital.

Methods: A cross-sectional survey study was conducted in a tertiary general hospital. Data were collected from 187 in-hospital nursing assistants using convenience sampling from June to July 2018. The Pittsburgh Sleep Quality Index (PSQI) was used to evaluate participants' sleep quality. A multiple linear regression was performed to identify associated factors with sleep quality.

Results: This study revealed a mean PSQI score of 5.96 ± 3.64 among all participants, of which 62.3% (114/187) participants suffered from impaired sleep quality. In-hospital nursing assistants with significant medical condition, low monthly income, irregular diet and high family burden reported more worse sleep quality.

Conclusion: The study showed that poor sleep quality is a highly prevalent issue among Chinese in-hospital nursing assistants in general hospital. Measures to enhance nursing assistants' wellbeing status (health and burden) and improve their salary (monthly income) are recommended.
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http://dx.doi.org/10.1016/j.heliyon.2022.e09393DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118489PMC
May 2022

A Two-eRNA-Based Signature Can Impact the Immune Status and Predict the Prognosis and Drug Sensitivity of Lung Adenocarcinoma.

J Immunol Res 2022 10;2022:8069858. Epub 2022 May 10.

Department of Oncology, Chongqing General Hospital, Chongqing, China.

Enhancer RNAs (eRNAs) are intergenic long noncoding RNAs (lncRNAs) participating in the development of malignant cancers via targeting cancer-associated genes and immune checkpoints. Immune infiltration of the tumor microenvironment was positively associated with overall survival (OS) in lung adenocarcinoma (LUAD). In this study, we aimed to explore the clinical significance of PCBP1-AS1 in LUAD and developed a novel prognostic signature based on two eRNAs. Our team discovered that the expression of PCBP1-AS1 was distinctly downregulated in LUAD specimens compared with nontumor specimens. Lower PCBP1-AS1 expression was related to advanced clinical stages and poor prognosis. KEGG analysis unveiled that the coexpression genes of PCBP1-AS1 were involved in the regulation of several tumor-related pathways. In addition, remarkable associations were observed between the expression of PCBP1-AS1 and the levels of several immune cells. Then, we used PCBP1-AS1 and TBX5-AS1 to develop a prognostic model. Survival assays unveiled that patients with higher risk scores exhibited a shorter OS in contrast to patients with lower risk scores. In addition, multivariable Cox regressive analysis indicated that the risk score was an independent prediction factor in LUAD sufferers. The anticancer drug sensitivity analysis indicated that risk score had a positive relationship with several anticancer drugs. Taken together, our findings indicated PCBP1-AS1 as a function modulator in LUAD development. In addition, we constructed a robust immune-related eRNA signature which might be a clinical prognosis factor for LUAD patients.
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http://dx.doi.org/10.1155/2022/8069858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115606PMC
May 2022

Fabrication of a Free-Standing MWCNT Electrode by Electric Field Force for an Ultra-Sensitive MicroRNA-21 Nano-Genosensor.

Small 2022 May 22:e2201791. Epub 2022 May 22.

Advanced Micro and Nano-Instruments Center, School of Mechanical & Automotive Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China.

Abnormal expression of microRNA-21 (miR-21) is considered to be closely associated with the pathogenesis of colorectal cancer. However, great challenges do exist for the development of ultra-sensitive biosensors to detect the abnormal expression of miR-21 due to the low concentration in serum (fm level) at the early stage of colorectal cancer. Therefore, electric field force is used to rotate and rearrange random multi-walled carbon nanotubes (MWCNTs) at the microscale to improve the active sites of the electrode in this study. The free-standing MWCNTs are densely and high-orderly embedded into the bare electrode along the direction of the electric field. Compared to the bare electrode, the peak-current response of the free-standing MWCNT electrode improves by 150 times in cyclic voltammetric measurement. A nano-genosensor based on the free-standing MWCNT electrode is developed for measuring miR-21. The nano-genosensor for miR-21 shows an ultra-high sensitivity of 48.24 µA µm , a wide linear range from 0.01 × 10 to 100 × 10 m, and a low detection limit of 1.2 × 10 m. The present nano-genosensor shows superior performance for miR-21 in human serum samples and demonstrates a potential application for the diagnosis of early stage colorectal cancer.
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http://dx.doi.org/10.1002/smll.202201791DOI Listing
May 2022

Sevoflurane preconditioning protects against acute MI/R injury via enhancing AdipoR1-Cav3 interaction and alleviating endoplasmic reticulum stress.

Exp Cell Res 2022 May 20;417(1):113217. Epub 2022 May 20.

Department of Anesthesiology and Center for Brain Science, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. Electronic address:

Whether and how sevoflurane preconditioning (SevoPre) exerts protection against acute myocardial ischemia/reperfusion (MI/R) injury remains elusive. We observed significant myocardial injury, as evidenced by infarct size, cardiomyocyte apoptosis, and circulating troponin-I, at 3 h of MI/R in both wildtype and adiponectin knockout mice. The injury was significantly ameliorated by SevoPre in wildtype mice, but not in adiponectin knockout mice. In wildtype mice, we found that MI/R could increase endoplasmic reticulum stress of cardiomyocytes, and impair association of adiponectin receptor 1 and ceveolin-3, both of which processes were largely restored by SevoPre. In summary, we demonstrated that significant injury had already took place at 3 h of MI/R, which could be ameliorated by SevoPre via promoting affinity of adiponectin receptor 1 and ceveolin-3, and then attenuating endoplasmic reticulum stress of cardiomyocytes.
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http://dx.doi.org/10.1016/j.yexcr.2022.113217DOI Listing
May 2022

How to advance China's carbon emission peak?- A comparative analysis of energy transition in China and the USA.

Environ Sci Pollut Res Int 2022 May 21. Epub 2022 May 21.

School of Mathematical Sciences, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.

This paper investigates carbon emission peak in China based on a comparative analysis of energy transition in China and the United States (US). The LMDI model is adopted to decompose carbon emissions into several driving factors in 2000-2018 for China and the US. Gray forecasting and NAR neural network are combined to predict peak time and identify optimal transition paths. The factor decomposition indicates that energy intensity is the main inhibitory factor for increased carbon emissions, while economic growth and population size are contributors for increased carbon emissions. There are significant differences in the impact of structure effect on carbon emissions in the two countries. The industry decomposition indicates that industry development is a critical inhibitor for increased carbon emissions after 2014 in China. The growth of transport and agriculture are basically contributing to increase carbon emissions in China and the US. The forecast results illustrate that China could complete carbon emission peak by 2030 under the baseline scenario, with a peak volume of 11354.72Mt CO. Under the industrial structure adjustment scenario, the carbon peak year may be advanced to 2028. While adjusting industrial structure and energy consumption structure at the same time, China could achieve carbon emission peak at 9918.21Mt CO in 2025.
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http://dx.doi.org/10.1007/s11356-022-20958-9DOI Listing
May 2022

Tumor-induced erythroid precursor-differentiated myeloid cells mediate immunosuppression and curtail anti-PD-1/PD-L1 treatment efficacy.

Cancer Cell 2022 May 16. Epub 2022 May 16.

Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Chongqing, China; Chongqing Key Laboratory of Immunotherapy, Chongqing, China. Electronic address:

Despite the unprecedented success of immune checkpoint inhibitors (ICIs) as anti-cancer therapy, it remains a prevailing clinical need to identify additional mechanisms underlying ICI therapeutic efficacy and potential drug resistance. Here, using lineage tracking in cancer patients and tumor-bearing mice, we demonstrate that erythroid progenitor cells lose their developmental potential and switch to the myeloid lineage. Single-cell transcriptome analyses reveal that, notwithstanding quantitative differences in erythroid gene expression, erythroid differentiated myeloid cells (EDMCs) are transcriptionally indistinguishable from their myeloid-originated counterparts. EDMCs possess multifaceted machinery to curtail T cell-mediated anti-tumor responses. Consequently, EDMC content within tumor tissues is negatively associated with T cell inflammation for the majority of solid cancers; moreover, EDMC enrichment, in accordance with anemia manifestation, is predictive of poor prognosis in various cohorts of patients undergoing ICI therapy. Together, our findings reveal a feedforward mechanism by which tumors exploit anemia-triggered erythropoiesis for myeloid transdifferentiation and immunosuppression.
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http://dx.doi.org/10.1016/j.ccell.2022.04.018DOI Listing
May 2022

Application of alginate dressing in the healing of chronic ulcers of lower limbs.

Minerva Med 2022 May 20. Epub 2022 May 20.

Wound Repair Ward, Zhengzhou Central Hospital, Zhengzhou, China.

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http://dx.doi.org/10.23736/S0026-4806.22.08072-7DOI Listing
May 2022

A Chromosome-Level Genome of the Camphor Tree and the Underlying Genetic and Climatic Factors for Its Top-Geoherbalism.

Front Plant Sci 2022 21;13:827890. Epub 2022 Apr 21.

Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.

Camphor tree [ (L.) J. Presl], a species in the magnoliid family Lauraceae, is known for its rich volatile oils and is used as a medical cardiotonic and as a scent in many perfumed hygiene products. Here, we present a high-quality chromosome-scale genome of with a scaffold N50 of 64.34 Mb and an assembled genome size of 755.41 Mb. Phylogenetic inference revealed that the magnoliids are a sister group to the clade of eudicots and monocots. Comparative genomic analyses identified two rounds of ancient whole-genome duplication (WGD). Tandem duplicated genes exhibited a higher evolutionary rate, a more recent evolutionary history and a more clustered distribution on chromosomes, contributing to the production of secondary metabolites, especially monoterpenes and sesquiterpenes, which are the principal essential oil components. Three-dimensional analyses of the volatile metabolites, gene expression and climate data of samples with the same genotype grown in different locations showed that low temperature and low precipitation during the cold season modulate the expression of genes in the terpenoid biosynthesis pathways, especially genes, which facilitates the accumulation of volatile compounds. Our study lays a theoretical foundation for policy-making regarding the agroforestry applications of camphor tree.
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http://dx.doi.org/10.3389/fpls.2022.827890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112071PMC
April 2022

Effect of feeding mid- or zero-tannin faba bean cultivars differing in vicine and covicine content on diet nutrient digestibility and growth performance of weaned pigs.

Transl Anim Sci 2022 Apr 18;6(2):txac049. Epub 2022 Apr 18.

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.

To prioritize what cultivars to grow to feed pigs, five faba bean cultivars including three zero-tannin, high vicine and covicine cultivars (Snowbird, Snowdrop, Tabasco), and two medium-tannin, lower vicine and covicine cultivars (Fabelle and Malik) were fed to compare effects on diet nutrient digestibility and growth performance of weaned pigs. A total of 260 pigs (8 ± 1.2 kg), weaned at 20 ± 1 d of age housed 2 barrows and 2 gilts/pen were fed 1 of 5 dietary regimens starting 1-week post-weaning for 4 weeks in a randomized complete block design. Diets including each cultivar at 20% or 30% provided 10.2 and 10.1 MJ net energy ()/kg and 1.3 and 1.2 g standardized ileal digestible () lysine ()/MJ NE in phases 1 and 2, respectively. Digestibility data were analyzed using PROC GLIMMIX and growth performance data were analyzed using PROC MIXED with pen as experimental unit. Fabelle contained the most condensed tannins (; 0.53%) but the least vicine (0.04%) and covicine (0.01%). Zero-tannin cultivars contained little CT (<0.2%) but had the greatest vicine (0.5%) and covicine content (0.4%). For phase 1, diet apparent total tract digestibility () of dry mater (), gross energy (), crude protein (), digestible energy (), and NE values did not differ among cultivars. For phase 2, diet ATTD of DM and GE were greatest ( < 0.05) for Snowdrop and Tabasco, intermediate for Fabelle, and lowest for Malik; Snowbird was not different from Fabelle or Malik. Diet ATTD of CP was greatest ( < 0.05) for Tabasco, intermediate for Snowbird, and lowest for Malik; Snowdrop was not different from Tabasco or Snowbird, and Fabelle was not different from Snowbird or Malik. Diet DE and NE values were greatest ( < 0.05) for Tabasco, intermediate for Fabelle and Snowdrop, and lowest for Snowbird; Malik was not different from Fabelle or Snowbird. For the entire trial (d 0-28), daily feed disappearance and weight gain for pigs fed Fabelle were 10% greater ( < 0.05) than those fed Malik; pigs fed zero-tannin cultivar diets were intermediate. Pigs fed Fabelle were 1.6 kg heavier ( < 0.05) than those fed Malik at the end of the trial; pigs fed zero-tannin cultivar diets were intermediate. In conclusion, growth performance of pigs fed faba bean cultivar diets was more related to feed disappearance than diet nutrient digestibility. Vicine and covicine instead of condensed tannin content of faba bean cultivars seemed more relevant to growth performance in weaned pigs.
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http://dx.doi.org/10.1093/tas/txac049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113422PMC
April 2022

5,7,2,5-tetrahydroxy-8,6-dimethoxyflavone up-regulates miR-145 expression and inhibits proliferation of gastric cancer cells.

Arch Med Sci 2022 13;18(3):753-760. Epub 2021 Apr 13.

Department of Oncology, Second People's Hospital of Dezhou, Decheng District, Dezhou, Shandong Province, China.

Introduction: Gastric cancer is a frequently detected malignancy, and its incidence has increased over the past decades in East Asia. The present study investigated the effect of 5,7,2, 5-tetrahydroxy-8,6-dimethoxyflavone (THDMF) on gastric cancer cells and explored the underlying mechanism. The study analysed cell viability changes, apoptotic features, and metastasis potential of treatment with THDMF.

Material And Methods: MTT colorimetric assay was used for measurement of MKN28, MKN45, and GES-1 cell proliferation and flow cytometry for the detection of apoptosis. Transwell and wound healing assays were used to observe the invasion and migration abilities of MKN28 cells. The expression of p21, MMP2/-9, PI3K, and c-Myc proteins was detected by western blotting.

Results: The THDMF treatment significantly ( < 0.05) reduced MKN28 and MKN45 cell proliferation without changing GES-1 cell viability. A significant increase in apoptotic cell population on treatment with THDMF was observed. Treatment of MKN28 cells with THDMF increased the percentage of cells in the G1 phase. Exposure of MKN28 cells to THDMF caused a marked decrease in invasion and migration potential in comparison to control cells. The expression of miR-145 was markedly increased in MKN28 cells on treatment with THDMF. In MKN28 cells expression of c-Myc, PI3K, p-AKT, MMP-2, and MMP-9 was suppressed markedly on exposure to THDMF. The expression of p21 protein in MKN28 cells was markedly promoted on exposure to THDMF.

Conclusions: THDMF exhibits anti-cancer effect on gastric cancer cells by activation of cell apoptosis and arrest of cell cycle. In addition, THDMF promoted miR-145 expression and down-regulation of PI3K/AKT signalling pathway in MKN28 cells. Therefore, THDMF may be utilised as a potential novel therapeutic agent for the treatment of gastric cancer.
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http://dx.doi.org/10.5114/aoms/121038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103526PMC
April 2021

Theoretical Prediction and Experimental Validation of the Glass-Forming Ability and Magnetic Properties of Fe-Si-B Metallic Glasses from Atomic Structures.

Materials (Basel) 2022 Apr 27;15(9). Epub 2022 Apr 27.

School of Materials Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.

Developing new soft magnetic amorphous alloys with a low cost and high saturation magnetization () in a simple alloy system has attracted substantial attention for industrialization and commercialization. Herein, the glass-forming ability (GFA), thermodynamic properties, soft magnetic properties, and atomic structures of FeSiB ( = 0-4) amorphous soft magnetic alloys were investigated by ab initio molecular dynamics (AIMD) simulations and experiments. The pair distribution function (PDF), Voronoi polyhedron (VP), coordination number (CN), and chemical short- range order (CSRO) were analyzed based on the AIMD simulations for elucidating the correlations between the atomic structures with the glass-forming ability and magnetic properties. For the studied compositions, the FeSiB amorphous alloy was found to exhibit the strongest solute-solute avoidance effect, the longest Fe-Fe bond, a relatively high partial CN for the Fe-Fe pair, and the most pronounced tendency to form more stable clusters. The simulation results indicated that FeSiB was the optimum composition balancing the saturation magnetization and the GFA. This prediction was confirmed by experimental observations. The presented work provides a reference for synthesizing new Fe-Si-B magnetic amorphous alloys.
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http://dx.doi.org/10.3390/ma15093149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103445PMC
April 2022

Noninvasive urinary protein signatures associated with colorectal cancer diagnosis and metastasis.

Nat Commun 2022 May 19;13(1):2757. Epub 2022 May 19.

State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Currently, imaging, fecal immunochemical tests (FITs) and serum carcinoembryonic antigen (CEA) tests are not adequate for the early detection and evaluation of metastasis and recurrence in colorectal cancer (CRC). To comprehensively identify and validate more accurate noninvasive biomarkers in urine, we implement a staged discovery-verification-validation pipeline in 657 urine and 993 tissue samples from healthy controls and CRC patients with a distinct metastatic risk. The generated diagnostic signature combined with the FIT test reveals a significantly increased sensitivity (+21.2% in the training set, +43.7% in the validation set) compared to FIT alone. Moreover, the generated metastatic signature for risk stratification correctly predicts over 50% of CEA-negative metastatic patients. The tissue validation shows that elevated urinary protein biomarkers reflect their alterations in tissue. Here, we show promising urinary protein signatures and provide potential interventional targets to reliably detect CRC, although further multi-center external validation is needed to generalize the findings.
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http://dx.doi.org/10.1038/s41467-022-30391-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9119985PMC
May 2022

TGF-β1/SH2B3 axis regulates anoikis resistance and EMT of lung cancer cells by modulating JAK2/STAT3 and SHP2/Grb2 signaling pathways.

Cell Death Dis 2022 May 19;13(5):472. Epub 2022 May 19.

Department of Thoracic Surgery, Affiliated Hospital of Jining Medical University, Jining Medical University, 272029, Jining, Shandong Province, P. R. China.

The pathogenesis of lung cancer, the most common cancer, is complex and unclear, leading to limited treatment options and poor prognosis. To provide molecular insights into lung cancer development, we investigated the function and underlying mechanism of SH2B3 in the regulation of lung cancer. We indicated SH2B3 was diminished while TGF-β1 was elevated in lung cancer tissues and cells. Low SH2B3 level was correlated with poor prognosis of lung cancer patients. SH2B3 overexpression suppressed cancer cell anoikis resistance, proliferation, migration, invasion, and EMT, while TGF-β1 promoted those processes via reducing SH2B3. SH2B3 bound to JAK2 and SHP2 to repress JAK2/STAT3 and SHP2/Grb2/PI3K/AKT signaling pathways, respectively, resulting in reduced cancer cell anoikis resistance, proliferation, migration, invasion, and EMT. Overexpression of SH2B3 suppressed lung cancer growth and metastasis in vivo. In conclusion, SH2B3 restrained the development of anoikis resistance and EMT of lung cancer cells via suppressing JAK2/STAT3 and SHP2/Grb2/PI3K/AKT signaling cascades, leading to decreased cancer cell proliferation, migration, and invasion.
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http://dx.doi.org/10.1038/s41419-022-04890-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120066PMC
May 2022
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