Publications by authors named "Li Wang"

9,423 Publications

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Incidence and risk factors for severe preeclampsia, HELLP syndrome and eclampsia at preterm and term gestation: a population-based study.

Am J Obstet Gynecol 2021 May 8. Epub 2021 May 8.

Department of Obstetrics and Gynaecology, University of British Columbia, and the Children's and Women's Hospital and Health Centre of British Columbia, Vancouver, BC, Canada; School of Population and Public Health, University of British Columbia, Vancouver, BC, Canada.

Background: A majority of previous studies of severe preeclampsia, eclampsia and HELLP (hemolysis, elevated liver enzymes and low platelets) syndrome were hospital-based or included a relatively small number of women. Large population-based studies examining gestational age-specific incidence patterns and risk factors for these severe pregnancy complications are lacking.

Objectives: To assess gestational age-specific incidence rates and risk factors for severe preeclampsia, HELLP syndrome, and eclampsia.

Methods: We carried out a retrospective population-based cohort study including all women with a singleton hospital birth in Canada (excluding Quebec), 2012/13-2015/16 (N=1,078,323). Data on the primary outcomes (i.e., severe preeclampsia, HELLP syndrome, and eclampsia) were obtained from delivery hospitalization records abstracted by the Canadian Institute of Health Information. Cox regression was used to assess independent risk factors (e.g., maternal age, chronic comorbidity) for each primary outcome, and to assess differences in effects at preterm vs. term gestation (<37 vs. ≥37 weeks).

Results: The rates of severe preeclampsia (n=2533), HELLP syndrome (n=2663), and eclampsia (n=465) were 2.35, 2.47 and 0.43 per 1000 singleton pregnancies, respectively. The cumulative incidence of term-onset severe preeclampsia was lower than that of preterm-onset severe preeclampsia (0.87 vs. 1.54 per 1000; rate ratio [RR]=0.57, 95% confidence intervals [CI]: 0.53-0.62), the rates of HELLP syndrome were similar (1.32 vs. 1.23 per 1000; RR=0.93, 95% CI: 0.86-1.00), and the preterm-onset eclampsia rate was lower than term-onset rate (0.12 vs. 0.33 per 1000; RR=2.64, 95% CI: 2.16-3.23). For each primary outcome, chronic comorbidity and congenital anomalies were stronger risk factors for preterm- vs. term-onset disease. Younger mothers (<25 years) had higher risk of severe preeclampsia at term, and eclampsia at all gestations, while older mothers (≥35 years) had elevated risks of severe preeclampsia and HELLP syndrome. Regardless of gestation, nulliparity was a risk factor for all outcomes, while socioeconomic status was inversely associated with severe preeclampsia.

Conclusion: The risk of severe preeclampsia declined at term, eclampsia risk increased at term and HELLP syndrome risk was similar at preterm and term gestation. Young maternal age was associated with increased risk of eclampsia and term-onset severe preeclampsia. Pre-pregnancy comorbidity and fetal congenital anomalies were more strongly associated with severe preeclampsia, HELLP syndrome, and eclampsia at preterm gestation.
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http://dx.doi.org/10.1016/j.ajog.2021.04.261DOI Listing
May 2021

Control of multiciliogenesis by miR-34/449 in the male reproductive tract through enforcing cell cycle exit.

J Cell Sci 2021 May 11;134(9). Epub 2021 May 11.

Department of Histoembryology, Genetics and Developmental Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.

Multiciliated cells (MCCs) are terminally differentiated postmitotic cells that possess hundreds of motile cilia on their apical surface. Defects in cilia formation are associated with ciliopathies that affect many organs. In this study, we tested the role and mechanism of the miR-34/449 family in the regulation of multiciliogenesis in EDs using an miR-34b/c-/-; miR-449-/- double knockout (dKO) mouse model. MiR-34b/c and miR-449 depletion led to a reduced number of MCCs and abnormal cilia structure in the EDs starting from postnatal day (P)14. However, abnormal MCC differentiation in the dKO EDs could be observed as early as P7. RNA-seq analyses revealed that the aberrant development of MCCs in the EDs of dKO mice was associated with the upregulation of genes involved in cell cycle control. Using a cyclin-dependent kinase inhibitor to force cell cycle exit promoted MCC differentiation, and partially rescued the defective multiciliogenesis in the EDs of dKO mice. Taken together, our results suggest that miR-34b/c and miR-449 play an essential role in multiciliogenesis in EDs by regulating cell cycle exit.
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http://dx.doi.org/10.1242/jcs.253450DOI Listing
May 2021

miR-133b inhibits cell proliferation, migration, and invasion of lung adenocarcinoma by targeting CDCA8.

Pathol Res Pract 2021 Apr 28;223:153459. Epub 2021 Apr 28.

Department of Cancer Radiotherapy and Chemotherapy, Zhejiang Quhua Hospital, Quzhou, 324004, China. Electronic address:

Objective: Lung adenocarcinoma (LUAD) is the most common type of lung cancer. This study aims to explore the mechanism by which CDCA8 regulates cell proliferation, invasion, and migration of LUAD, and to generate novel insights into targeted therapy of LUAD.

Methods: Expression profiles of mature microRNAs (miRNAs) and mRNAs, along with clinical data of LUAD were downloaded from TCGA database for differential analysis and survival analysis to mine differentially expressed mRNAs. qRT-PCR was used to detect the expression of CDCA8 and miR-133b in LUAD cell lines, and western blot was used to detect protein expression. The effects of CDCA8 on the proliferation, migration, and invasion of LUAD cells were detected by CCK-8 assay, scratch healing assay, and Transwell assay. Bioinformatics predicted the target miRNA of CDCA8, and dual-luciferase reporter gene assay was used to verify the binding relationship between miR-133b and CDCA8.

Results: Data from TCGA-LUAD showed that CDCA8 was significantly overexpressed in LUAD tissue, while its upstream miRNA (miR-133b) was significantly lowly expressed. The result of dual-luciferase test showed that miR-133b targeted CDCA8. The results of in vitro functional experiments showed that overexpression of CDCA8 could promote the proliferation, invasion, and migration of LUAD cells, and miR-133b could reverse this promotion by targeting CDCA8.

Conclusion: This study found that CDCA8 was a carcinogenic factor in LUAD cells and it was regulated by upstream miR-133b. miR-133b could inhibit proliferation, invasion, and migration of LUAD cells by targeting CDCA8.
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http://dx.doi.org/10.1016/j.prp.2021.153459DOI Listing
April 2021

Surface Selenylation Engineering for Construction of a Hierarchical NiSe/Carbon Nanorod: A High-Performance Nonenzymatic Glucose Sensor.

ACS Appl Mater Interfaces 2021 May 10. Epub 2021 May 10.

College of Food Science and Engineering, Northwest A&F University, Yangling, 712100 Xianyang, Shaanxi, China.

As glucose (Glu) is an essential substance for metabolism as well as a symbol to diagnose diabetes, the demand of Glu sensors has increased significantly in recent decades. In this work, a hierarchical Ni-based electrochemical enzyme-free Glu sensor, namely, NiSe/CNR (carbon nanorod), was engineered through a facile thermal treatment using dimethylglyoxime dinickel salt with selenium (Se) powder. The prepared NiSe/CNR not only subtly introduces a hierarchical structure with rod-like carbon nanorods and rock-like NiSe nanoparticles, which are extremely helpful in offering a greater catalytic activity area and more catalytic active sites, but also incorporates the Se element to increase the inherent activity. The fabricated NiSe/CNR exhibits distinguished performance for Glu detection in alkaline electrolytes with linear ranges of 0.5-411 μM and 411 μM to 6.311 mM, high sensitivities of 3636 μA mM cm at low concentrations, and 2121 μA mM cm at high concentrations, as well as a low detection limit of 380 nM (S/N = 3). It also possesses favorable reproducibility, stability, and long-term storage capacity. The practical feasibility of NiSe/CNR was also validated by detecting Glu in human serum. Moreover, the prepared hierarchical NiSe/CNR is of general interest for the construction of hierarchical Ni-based sensors.
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http://dx.doi.org/10.1021/acsami.1c04831DOI Listing
May 2021

The association between variants in PLA2R and HLA-DQA1 and renal outcomes in patients with primary membranous nephropathy in Western China.

BMC Med Genomics 2021 May 8;14(1):123. Epub 2021 May 8.

Department of Nephrology and Institute of Nephrology, Sichuan Academy of Medical Science and Sichuan Provincial People's Hospital, Chengdu, 610072, China.

Background: Both Genome-wide associations and our previous study have shown that single nucleotide polymorphisms (SNPs) of M-type phospholipase A2 receptor (PLA2R) and human leukocyte antigen complex class II HLA-DQα-chain 1 (HLA-DQA1) gene were identified to be associated with primary membranous nephropathy (PMN). However, whether these SNPs affect clinical manifestation and renal outcome for PMN patients is poorly defined. Here, we evaluated whether there is an association between these SNPs and clinical manifestations and renal outcomes of PMN in a western Chinese cohort.

Methods: Seven SNPs within PLA2R and one SNP in HLA-DQA1 were selected in our study. Clinical data from 314 patients with PMN were collected and the relationship between the genotype and phenotype was evaluated. A total of 186 patients had follow-up data. We assessed the treatment responses and renal outcomes between patients with these gene polymorphisms after a median follow-up of 18.6 months.

Results: Eight SNPs were not associated with clinical manifestations of PMN patients (Pc < 0.05). rs3828323 T allele was marginally significantly associated with hypertension (P = 0.008, Pc = 0.064, OR = 1.821). After treatment for PMN, the SR group (including CR and PR) had lower serum creatinine level (68.4 ± 18.8 μmol/L vs. 122.8 ± 126.6 μmol/L, P < 0.001), urea (5.5 ± 1.9 mmol/L vs. 8.0 ± 4.0 mmol/L, P < 0.001), uric acid (358.5 ± 95.1 μmol/L vs. 392.8 ± 118.1 μmol/L, P = 0.037) and urinary protein (0.23 (0.76,1.05) g/d vs. 3.01 (2.06,7.95) g/d, P < 0.001), higher eGFR (100.0 ± 20.1 ml/min/1.73m vs. 77.1 ± 35.3 ml/min/1.73m, P < 0.001) and albumin (41.1 ± 5.1 g/L vs.30.4 ± 8.2 g/L, P < 0.001). We also identified that PMN patients with CT/TT genotype for rs3828323 achieved higher cumulative survival rate than patients with CC genotype.

Conclusions: Rs3828323 may influence hypertension and renal outcome in patients with PMN. Further research is needed to explore the mechanism for this genotype-disease phenotype association.
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http://dx.doi.org/10.1186/s12920-021-00969-0DOI Listing
May 2021

Correction to: Angiomotin-p130 inhibits vasculogenic mimicry formation of small cell lung cancer independently of Smad2/3 signal pathway.

J Bioenerg Biomembr 2021 May 7. Epub 2021 May 7.

Department of Medical Imaging, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, People's Republic of China.

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http://dx.doi.org/10.1007/s10863-021-09896-2DOI Listing
May 2021

Focus on the Mental Health of Pediatric Medical Workers in China After the COVID-19 Epidemic.

Authors:
Hui Liu Li Wang

Front Psychol 2021 20;12:657814. Epub 2021 Apr 20.

Department of Pediatrics, University-Town Hospital of Chongqing Medical University, Chongqing, China.

As was previously known, pediatric medical staff in China faced several hurdles including high occupational risk, multiple contradictions, heavy workload, and long working hours. After the outbreak of 2019 novel coronavirus, facing the overload of work and the potential risk of infection, pediatric medical workers may be under great psychological pressure. The purpose of this article was to call attention to the impact of the epidemic on the mental health of Chinese pediatric workers, and developing psychological intervention program that are tailored to them. The experiences from this public health emergency should inform the efficiency and quality of future crisis intervention of the Chinese government and authorities around the world.
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http://dx.doi.org/10.3389/fpsyg.2021.657814DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093376PMC
April 2021

Deriving cut-off values for continuous predictors of severe outcomes in COVID-19 through meta-analysis of individual studies: A comment on the article by Hariyanto et al.

Am J Emerg Med 2021 Mar 14. Epub 2021 Mar 14.

School of Biomedical Engineering & Imaging Sciences, King's College London, London, UK; Department of Clinical Therapeutics, National and Kapodistrian University of Athens School of Medicine, Athen, Greece. Electronic address:

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http://dx.doi.org/10.1016/j.ajem.2021.03.029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7955938PMC
March 2021

Diverse data augmentation for learning image segmentation with cross-modality annotations.

Med Image Anal 2021 Apr 20;71:102060. Epub 2021 Apr 20.

Department of Radiology and Biomedical Research Imaging Center (BRIC), University of North Carolina, Chapel Hill, NC, USA. Electronic address:

The dearth of annotated data is a major hurdle in building reliable image segmentation models. Manual annotation of medical images is tedious, time-consuming, and significantly variable across imaging modalities. The need for annotation can be ameliorated by leveraging an annotation-rich source modality in learning a segmentation model for an annotation-poor target modality. In this paper, we introduce a diverse data augmentation generative adversarial network (DDA-GAN) to train a segmentation model for an unannotated target image domain by borrowing information from an annotated source image domain. This is achieved by generating diverse augmented data for the target domain by one-to-many source-to-target translation. The DDA-GAN uses unpaired images from the source and target domains and is an end-to-end convolutional neural network that (i) explicitly disentangles domain-invariant structural features related to segmentation from domain-specific appearance features, (ii) combines structural features from the source domain with appearance features randomly sampled from the target domain for data augmentation, and (iii) train the segmentation model with the augmented data in the target domain and the annotations from the source domain. The effectiveness of our method is demonstrated both qualitatively and quantitatively in comparison with the state of the art for segmentation of craniomaxillofacial bony structures via MRI and cardiac substructures via CT.
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http://dx.doi.org/10.1016/j.media.2021.102060DOI Listing
April 2021

Pretreatment whole blood Epstein-Barr virus DNA predicts prognosis in Hodgkin lymphoma.

Leuk Res 2021 Apr 30;107:106607. Epub 2021 Apr 30.

Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, 210029, China; Key Laboratory of Hematology of Nanjing Medical University, Nanjing, 210029, China; Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing 210029, China. Electronic address:

The study investigated pretreatment Epstein-Barr virus (EBV) DNA status and its prognostic values in 96 patients newly diagnosed Hodgkin lymphoma (HL). With 13.5 % patients in positive EBV DNA status before therapy, the positive group had inferior progression-free survival (PFS) (P = 0.023) as well as overall survival (OS) (P = 0.001). Pretreatment EBV DNA positivity was observed as an independent prognostic factor in OS (P = 0.036) while a trend to predict PFS (P = 0.064). By monitoring changes of EBV DNA copies in 13 patients with positive pretreatment EBV DNA status, 5 of 6 patients with complete response (CR) had their copies undetectable after 3 cycles of first-line treatment and 7 patients with progressive disease (PD) all had elevated EBV DNA copies during their relapsed period. Whole blood EBV DNA may be an adjunctive biomarker to reflect treatment response, risk of disease relapse as well as prognosis in HL patients.
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http://dx.doi.org/10.1016/j.leukres.2021.106607DOI Listing
April 2021

The antimicrobial activity of silver nanoparticles biocomposite films depends on the silver ions release behaviour.

Food Chem 2021 Apr 28;359:129859. Epub 2021 Apr 28.

Food Quality and Design, Bornse Weilanden 9, 6708 WG, Wageningen, The Netherlands. Electronic address:

This study aimed to investigate the release of silver ions from the packaging, their diffusion within a food hydrogel and the effect on the growth of Pseudomonas fluorescens. Biosorbed-silver nanoparticles (BSNPs) were synthesized using a plant extract and were incorporated into chitosan or poly (vinyl alcohol) polymer to prepare biocomposite films. The addition of BSNPs improved the physical and antimicrobial properties of the films as shown by tensile strength and inhibition of P. fluorescens in hydrogels, respectively. PVA based BSNPs film showed a stronger antimicrobial effect, compared to chitosan based BSNPs film and this correlated with a higher amount of silver ions release from the PVA film into the hydrogel. Results suggest that the strength of the interaction between BSNPs and the film polymer is the key factor leading to the difference in the release behaviour of the antimicrobials, which in turn determines the antimicrobial activity of the active packaging.
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http://dx.doi.org/10.1016/j.foodchem.2021.129859DOI Listing
April 2021

Preparation of conductive cellulose fabrics with durable antibacterial properties and their application in wearable electrodes.

Int J Biol Macromol 2021 May 3;183:651-659. Epub 2021 May 3.

School of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address:

Electroless silver plating on fabrics can obtain conductive and antibacterial bifunctional materials which can be used as electrodes in wearable electronic products. However, these activities are deteriorated easily after washing because of the falling off of silver coating resulted from the weak adhesion. In order to improve the binding force between silver and cellulose fabrics, 3-mercaptopropytrimethoxysilane (MPTS) was applied to modify cellulose fabrics before silver electroless plating to develop the durable conductive fabrics with excellent antibacterial. The silver nanoparticles (Ag NPs) deposition process was observed via field emission scanning electron microscopy (FESEM), thermal properties were evaluated by thermogravimetric analysis (TGA). A dense and uniform silver layer was formed on the fabric. The initial electrical resistance of the conductive fabric was 0.04 Ω/sq and lowered than 2 Ω/sq after 200 washing cycles. The antibacterial efficiency of the fabric after 200 washing cycles remained 92.82%, compared to 100% with the fabric before washing. Moreover, the inhibition rate was determined by optical density of bacteria suspension at 260 nm and further substantiated by releasing of Ag from the fabric. The conductive fabrics were applied as wearable electrodes to capture electrocardiogram (ECG) signals of human in static states and running states.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.04.176DOI Listing
May 2021

Anlotinib exerts anti-cancer efficiency on lung cancer stem cells in vitro and in vivo through reducing NF-κB activity.

J Cell Mol Med 2021 May 6. Epub 2021 May 6.

Lung Cancer Center, State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu, China.

Anlotinib is a multi-target tyrosine kinase inhibitor. Previous studies confirmed that anlotinib exerts anti-cancer efficiency. However, the functional roles of anlotinib on cancer stem cells (CSCs) are yet to be elucidated. In this study, lung CSCs were isolated and identified in vitro, and mouse xenografts were established in vivo. MTT assays, tumour sphere formation assays, TdT-mediated dUTP nick-end labelling (TUNEL) staining, Annexin V-FITC/PI staining, immunofluorescence analysis and Western blot were performed to investigate the anti-cancer effects of anlotinib on lung CSCs. The results showed that anlotinib inhibits the growth of lung CSCs in vitro and in vivo. In addition, anlotinib induced apoptosis of these cells along with down-regulated expression level of Bcl-2 whereas up-regulated Bax and cleaved caspase-3 expression. It also sensitized lung CSCs to the cytotoxicity of cisplatin and paclitaxel; the tumour sphere formation and expression levels of multiple stemness-associated markers, such as ALDH1 and CD133, were also decreased. Furthermore, the underlying mechanism indicated that anlotinib reduces the phosphorylated levels of NF-κB p65 and IκB-α in lung CSCs. Taken together, these findings suggested that anlotinib exerts potent anti-cancer effects against lung CSCs through apoptotic induction and stemness phenotypic attenuation. The mechanism could be associated with the suppression of NF-κB activity.
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http://dx.doi.org/10.1111/jcmm.16564DOI Listing
May 2021

Nitrite-enhanced copper-based Fenton reactions for biofilm removal.

Chem Commun (Camb) 2021 May 6. Epub 2021 May 6.

Department of Chemistry, Cape Breton University, Sydney, Nova Scotia B1P 6L2, Canada.

Unwanted biofilms present challenges for many industries. Herein an innovative biofilm removal technology was developed based on nitrite-accelerated Fenton chemistry, where both dissolved Cu ions and nano-CuO surfaces efficiently generate reactive nitrogen species as disinfectants. This simple, efficient, and cost-effective approach for biofilm removal generates important insights into Fenton chemistry, a fundamental mechanism in nature, considering the ubiquity of copper, hydrogen peroxide, and nitrite in the environment, biological systems, and various industrial processes.
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http://dx.doi.org/10.1039/d1cc00374gDOI Listing
May 2021

Renoprotective Effect of Oridonin in a Mouse Model of Acute Kidney Injury via Suppression of Macrophage Involved Inflammation.

Biol Pharm Bull 2021 ;44(5):714-723

Research Center for Integrated Chinese and Western Medicine, Affiliated Traditional Medicine Hospital, Southwest Medical University.

Ischemia-reperfusion injury (IRI) is the major cause of acute kidney injury (AKI). The previous studies demonstrated that Oridonin can protect kidney against IRI-induced AKI, but the underlying molecular mechanism is unclear. In this study, it showed that Oridonin significantly improved kidney damage, and inhibited the expression of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α and MCP-1, as well as macrophage marker F4/80 in kidney and the secretion of inflammatory cytokins in serum of AKI mice in vivo. In addition, Oridonin also effectively reduced the expression and secretion of lipopolysaccharide (LPS)-induced inflammatory factors in macrophage cell line RAW264.7 in vitro. Notably, Oridonin strongly downregulated Mincle and AKT/nuclear factor-kappaB (NF-κB) signaling both in vivo and in vitro, and the results of cellular recovery experiments of overexpression of Mincle in macrophage suggested that Oridonin suppressed inflammatory response of macrophage through inhibiting Mincle, which may be the underlying mechanism of Oridonin improving injury in kidney of AKI mice. In summary, the above results indicated that Oridonin can protect kidney from IRI-induced inflammation and injury by inhibiting the expression of Mincle in macrophage.
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http://dx.doi.org/10.1248/bpb.b21-00071DOI Listing
January 2021

Comparison of Different Soluble Dietary Fibers during the Fermentation Process.

J Agric Food Chem 2021 May 5. Epub 2021 May 5.

School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Soluble dietary fibers being fermented by gut microbiota constitute a pivotal prerequisite for soluble dietary fibers exhibiting physiological functions. However, the relationship between fiber type and gut microbiota metabolism remains unclear. The purpose of this study was to investigate and compare the effect of fiber types on short-chain fatty acid (SCFA) biosynthesis in a simulated colon. Results showed that different soluble dietary fibers caused distinct metabolic profiles both in SCFAs and organic acids. Further analysis revealed that the SCFA biosynthesis pathway was related to the chain structure of fiber polysaccharides. Moreover, the microbial community structure showed substantial difference among experimental groups. was substantially elevated in the resistant starch group, while was the predominant genus in other groups. Correlation analysis further revealed that SCFA biosynthesis was correlated with microbial taxa at different taxonomic levels. Totally, the present study provided an insight into targeted intervention of gut microorganisms for dictating SCFA and organic acid production.
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http://dx.doi.org/10.1021/acs.jafc.1c00237DOI Listing
May 2021

Three coordination polymers built by quaternary-ammonium-modified isophthalic acid.

Acta Crystallogr C Struct Chem 2021 May 9;77(Pt 5):221-226. Epub 2021 Apr 9.

Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, Jiangsu Province 211800, People's Republic of China.

Three coordination polymers based on quaternary-ammonium-modified isophthalic acid, namely, catena-poly[[[aqua-μ-bromido-di-μ-hydroxido-methanoldinitratotetracopper(II)]-bis{μ-5-[2-(tripropylazaniumyl)ethoxy]benzene-1,3-dicarboxylato}] nitrate], {[CuBr(CHNO)(NO)(OH)(CHO)(HO)]NO}, 1, poly[μ-bromido-μ-bromido-bromido-μ-hydroxido-{μ-5-[2-(tripropylazaniumyl)ethoxy]benzene-1,3-dicarboxylato}tricopper(II)], [CuBr(CHNO)(OH)], 2, and poly[bromido{μ-5-[2-(tripropylazaniumyl)ethoxy]benzene-1,3-dicarboxylato}zinc(II)], [ZnBr(CHNO)], 3, were obtained by solvothermal reactions. Coordination polymer (CP) 1 contains tetranuclear Cu units, in which the four Cu atoms are linked by two μ-OH groups into a Cu(OH) cluster, which are in turn linked by 5-[2-(tripropylazaniumyl)ethoxy]benzene-1,3-dicarboxylate (cpa) ligands into a chain structure. CP 2 also contains a tetranuclear Cu(OH) cluster and these are linked with CuBr units into chains. The chains are then connected by cpa ligands into a two-dimensional layered structure. CP 3 contains a two-dimensional layer structure built by binuclear Zn units and cpa ligands. The Br counter-anions of the quaternary ammonium groups all take part in the construction of the polymeric networks.
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http://dx.doi.org/10.1107/S2053229621003296DOI Listing
May 2021

Erratum: Epidemiology of and prognostic factors for patients with sarcomatoid carcinoma: a large population-based study.

Am J Cancer Res 2021 15;11(4):1800-1802. Epub 2021 Apr 15.

Lung Cancer Center, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital of Sichuan University Chengdu, Sichuan, P. R. China.

[This corrects the article on p. 3801 in vol. 10, PMID: 33294268.].
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085883PMC
April 2021

Combined inhibition of Rad51 and Wee1 enhances cell killing in HNSCC through induction of apoptosis associated with excessive DNA damage and replication stress.

Mol Cancer Ther 2021 May 4. Epub 2021 May 4.

Head and Neck Surgery, The University of Texas MD Anderson Cancer Center.

Despite advances in surgery, chemotherapy, and radiation, there are limited treatment options for advanced head and neck squamous cell carcinoma (HNSCC) and survival remains very poor. Therefore, effective therapies are desperately needed. Recently, selective exploitation of DNA damage and replication stress responses has become a novel approach for cancer treatment. Wee1 kinase and Rad51 recombinase are two proteins involved in regulating replication stress and homologous recombination repair in cancer cells. In this study, we investigated the combined effect of Rad51 inhibitor (B02) and Wee1 inhibitor (AZD1775) in vitro and in vivo in various HNSCC cell lines. Clonogenic survival assays demonstrated that B02 synergized with AZD1775 in vitro in all HNSCC cell lines tested. The synergy between these drugs was associated with forced CDK1 activation and reduced Chk1 phosphorylation leading to induction of excessive DNA damage and replication stress, culminating in aberrant mitosis and apoptosis. Our results showed that elevated Rad51 mRNA expression correlated with worse survival in HNSCC patients with HPV-positive tumors. The combination of B02 and AZD1775 significantly inhibited tumor growth in vivo in mice bearing HPV-positive HNSCC tumors as compared to HPV-negative HNSCC. This differential sensitivity appears to be linked to HPV-positive tumors having more in vivo endogenous replication stress owing to transformation by E6 and E7 oncogenes. Furthermore, addition of B02 radiosensitized the HPV-negative HNSCC tumors in vitro and in vivo. In conclusion, our data implicate that a novel rational combination with Rad51 and Wee1 inhibitors holds promise as synthetic lethal therapy, particularly in high-risk HPV-positive HNSCC.
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http://dx.doi.org/10.1158/1535-7163.MCT-20-0252DOI Listing
May 2021

Diagnostic and therapeutic potencies of miR-18a-5p in mixed-type gastric adenocarcinoma.

J Cell Biochem 2021 May 4. Epub 2021 May 4.

Department of Thoracic Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.

Mixed-type gastric adenocarcinoma (by Lauren Classification) has poor clinical outcomes with few targeted treatment options. The primary objective of this study was to find the prognostic factors, accurate treatment approaches, and effective postoperative adjuvant therapy strategies for patients with mixed-type gastric adenocarcinoma (GA). A microRNA sequencing data set and the corresponding clinical parameters of patients with gastric cancer were obtained from The Cancer Genome Atlas. Differentially expressed microRNAs (DEMs) of diffuse- and intestinal-type GA were, respectively, determined. Kaplan-Meier and log-rank tests were subsequently carried out to evaluate the prognostic relevance of each DEM. To study the common factors between diffuse- and intestinal-type GA, a pathway enrichment analysis was performed on the target genes of identified DEMs using the PANTHER database. After data preprocessing, we analyzed a total of 230 samples from 210 patients with GA. Eighty-six DEMs in diffuse-type GA samples and 59 DEMs in intestinal-type GA samples were, respectively, identified (p  2.0). The Kaplan-Meier survival method further screened out six prognosis-related DEMs for diffuse-type GA and seven prognosis-related DEMs for intestinal-type GA (p < 0.05). MiR-18a-5p was found to be the only common prognosis-related DEM between diffuse- and intestinal-type GA. The common signaling pathways further revealed that target genes of miR-18a-5p are involved in mixed-type GA progression. This study suggests that miR-18a-5p acts as a potential target for treatment, and common signal pathways provide a rich basis to seek reliable and effective molecular targets for the diagnosis, clinical treatment, and postoperative adjuvant therapy strategy of mixed-type GA.
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http://dx.doi.org/10.1002/jcb.29927DOI Listing
May 2021

Development of BRAF V600E Mutation in NRAS Q61L Mutated Rectal Cancer.

Ann Clin Lab Sci 2021 Mar;51(2):262-266

Laboratory of Molecular Medicine, Ordos Central Hospital, Ordos

BRAF and NRAS are oncogenes in the RAS/RAF/MEK/MAP-kinase signaling pathway. Coexistent mutations of BRAF and NRAS in a single colorectal cancer patient have always been considered mutually exclusive or at least rare. The clinical outcome of these patients remains undetermined. Herein we report a 53-year-old man harboring an NRAS Q61L mutation in his primary rectal carcinoma, who presented with a concomitant mutation of BRAF V600E in his liver metastasis biopsy 55 months after the primary CRC surgical resection. Our findings suggest that a BRAF and NRAS developed co-mutation may lead to a distinct clinicopathological progression. BRAF-mutated CRCwill not benefit from anti-RAS targeted therapy.
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March 2021

Corrigendum to Investigations of EGFR configurations on tumor cell surface by high-resolution electron microscopy.

Biochem Biophys Res Commun 2021 Jun 30;558:239. Epub 2021 Apr 30.

Beijing Key Laboratory of Microstructure and Properties of Solids, Institute of Microstructure and Property of Advanced Materials, Beijing University of Technology, Beijing, 100124, China. Electronic address:

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http://dx.doi.org/10.1016/j.bbrc.2021.04.023DOI Listing
June 2021

Wheat bran, as the resource of dietary fiber: a review.

Crit Rev Food Sci Nutr 2021 May 3:1-28. Epub 2021 May 3.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, China.

Wheat bran is a major by-product of white flour milling and had been produced in large quantities around the world; it is rich in dietary fiber and had already been used in many products such as whole grain baking or high dietary fiber addition. It has been confirmed that a sufficient intake of dietary fiber in wheat bran with appropriate physiological functions is beneficial to human health. Wheat bran had been considered as the addition with a large potential for improving the nutritional condition of the human body based on the dietary fiber supplement. The present review summarized the available information on wheat bran related to its dietary fiber functions, which may be helpful for further development of wheat bran as dietary fiber resource.
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http://dx.doi.org/10.1080/10408398.2021.1913399DOI Listing
May 2021

Puerarin Restores Autophagosome-Lysosome Fusion to Alleviate Cadmium-Induced Autophagy Blockade via Restoring the Expression of Rab7 in Hepatocytes.

Front Pharmacol 2021 14;12:632825. Epub 2021 Apr 14.

College of Veterinary Medicine, Yangzhou University, Yangzhou, China.

Autophagic dysfunction is one of the main mechanisms by which the environmental pollutant cadmium (Cd) induces cell injury. Puerarin (Pue, a monomeric Chinese herbal medicine extract) has been reported to alleviate Cd-induced cell injury by regulating autophagy pathways; however, its detailed mechanisms are unclear. In the present study, to investigate the detailed mechanisms by which Pue targets autophagy to alleviate Cd hepatotoxicity, alpha mouse liver 12 (AML12) cells were used to construct a model of Cd-induced hepatocyte injury . First, the protective effect of Pue on Cd-induced cell injury was confirmed by changes in cell proliferation, cell morphology, and cell ultrastructure. Next, we found that Pue activated autophagy and mitigated Cd-induced autophagy blockade. In this process, the lysosome was further activated and the lysosomal degradation capacity was strengthened. We also found that Pue restored the autophagosome-lysosome fusion and the expression of Rab7 in Cd-exposed hepatocytes. However, the fusion of autophagosomes with lysosomes and autophagic flux were inhibited after knocking down , and were further inhibited after combined treatment with Cd. In addition, after knocking down , the protective effects of Pue on restoring autophagosome-lysosome fusion and alleviating autophagy blockade in Cd-exposed cells were inhibited. In conclusion, Pue-mediated alleviation of Cd-induced hepatocyte injury was related to the activation of autophagy and the alleviation of autophagy blockade. Pue also restored the fusion of autophagosomes and lysosomes by restoring the protein expression of Rab7, thereby alleviating Cd-induced autophagy blockade in hepatocytes.
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http://dx.doi.org/10.3389/fphar.2021.632825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079953PMC
April 2021

Effect of phosphate salts on the gluten network structure and quality of wheat noodles.

Food Chem 2021 Apr 20;358:129895. Epub 2021 Apr 20.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. Electronic address:

The effects of three phosphate salts (PS) on the secondary structure, microstructure of gluten, rheological properties of dough and water status of noodles were investigated to determine the mechanisms underlying the changes in the quality of noodles. Changes in the secondary structure detected were the increased number of β-sheet and decreased number of random coil structures. PS reduced the content of free sulfhydryl (SH) and increased the content of disulfide (SS) bonds. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis indicated that the band density of the high molecular regions of the gluten was reduced. The results showed that adding PS induced a more compact microstructure and improved the G' and G'' values of the dough. After adding PS, the water-solids interaction in noodles was enhanced by the decreased water mobility. It was concluded that PS promoted the water holding capacity of the noodles and strengthened the gluten network.
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http://dx.doi.org/10.1016/j.foodchem.2021.129895DOI Listing
April 2021

Physicochemical and digestive properties of A- and B-type granules isolated from wheat starch as affected by microwave-ultrasound and toughening treatment.

Int J Biol Macromol 2021 Apr 29;183:481-489. Epub 2021 Apr 29.

School of Food Science, Jiangnan University, Wuxi 214000, China.

In this study, the effect of microwave-ultrasound or/and toughening treatment on the physicochemical, structural properties, and in vitro digestibility of A- and B-type granules isolated from wheat starch were investigated. From the SEM, microwave-ultrasound and toughening treatment (MU-T) led to the appearance of irregular and disrupted structure significantly and an increment in the resistant starch content of A- and B-type granule. Furthermore, the MU-T starch possessed the lowest swelling power, light transmittance, and gelatinization temperature range (T -T) and the highest ΔH. After MU-T, the relative crystallinity (RC) of X-ray pattern, Fourier transform infrared ratio of 1047/1022 cm, and the content of double helix and single helix of C CP/MAS NMR had increased significantly. In particular, there was a difference in the content of RS and SDS between A-starch granules and B-starch granules as well as their changes after modification (from 69.305% to 82.93 for A-starch and form 74.97% to 88.17 for B-starch, respectively), which was a similar trend with RC and helix content. This study indicated that, for both A-type granule and B-type granule starches, microwave-ultrasound and toughening treated samples had unique properties compared to singly modified starches.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.04.180DOI Listing
April 2021

Profibrotic mechanisms of DPP8 and DPP9 highly expressed in the proximal renal tubule epithelial cells.

Pharmacol Res 2021 Apr 29;169:105630. Epub 2021 Apr 29.

Department of Nephrology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710005, China. Electronic address:

Background: DPP8 and DPP9 have been demonstrated to play important roles in multiple diseases. Evidence for increased gene expression of DPP8 and DPP9 in tubulointerstitium was found to be associated with the decline of kidney function in chronic kidney disease (CKD) patients, which was observed in the Nephroseq human database. To examine the role of DPP8 and DPP9 in the tubulointerstitial injury, we determined the efficacy of DPP8 and DPP9 on epithelial-to-mesenchymal transition (EMT) and tubulointerstitial fibrosis (TIF) as well as the underlying mechanisms.

Methods: We conducted the immunofluorescence of DPP8 and DPP9 in kidney biopsy specimens of CKD patients, established unilateral ureteral obstruction (UUO) animal model, treated with TC-E5007 (a specific inhibitor of both DPP8 and DPP9) or Saxagliptin (positive control) or saline, and HK-2 cells model.

Results: We observed the significantly increased expression of DPP8 and DPP9 in the renal proximal tubule epithelial cells of CKD patients compared to the healthy control subjects. DPP8/DPP9 inhibitor TC-E5007 could significantly attenuate the EMT and extracellular matrix (ECM) synthesis in UUO mice, all these effects were mediated via interfering with the TGF-β1/Smad signaling. TC-E5007 treatment also presented reduced renal inflammation and improved renal function in the UUO mice compared to the placebo-treated UUO group. Furthermore, the siRNA for DPP8 and DPP9, and TC-E5007 treatment decreased EMT- and ECM-related proteins in TGF-β1-treated HK-2 cells respectively, which could be reversed significantly by transduction with lentivirus-DPP8 and lentivirus-DPP9.

Conclusion: These data obtained provide evidence that the DPP8 and DPP9 could be potential therapeutic targets against TIF.
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http://dx.doi.org/10.1016/j.phrs.2021.105630DOI Listing
April 2021

Expression and change of miR-199b-5p, s HLA-G in thyroid carcinoma.

Exp Mol Pathol 2021 Apr 28;120:104643. Epub 2021 Apr 28.

Department of Nuclear Medicine, the Affiliated Hospital of Southwest Medical University, Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, Luzhou 646000, Sichuan Province, China. Electronic address:

Objective: This research aims to investigate the relationship between the expression of microRNA-199b-5p (miR-199b-5p) and soluble human leukocyte antigen G (sHLA-G) in thyroid cancer tissues and its clinicopathological characteristics, as well as its impact on prognosis.

Methods: Frozen tissues and serum from 85 patients with thyroid cancer, 27 with thyroid adenoma, 19 with Hashimoto's thyroiditis and 14 with nodular goiter from February 2014 to March 2016 were sampled. The miR-199b-5pmRNA expression in tissues was analyzed by real-time quantitative PCR. Serum HLA-G expression was detected by ELISA, and the relationship between s HLA-G expression and clinicopathological characteristics of thyroid cancer was analyzed. The relationship between 1- and 3-year survival rates of all patients and the expression of both detection indexes was observed.

Results: Compared with normal thyroid specimens, nodular goiter, Hashimoto's thyroiditis, thyroid adenoma and thyroid cancer patients, the relative expression of miR-199b-5pmRNA in thyroid cancer tissues was the lowest, while that of s HLA-G was the highest in serum of patients (P < 0.05). The levels of miR-199b-5pmRNA and serum s HLA-G in tumor tissues were correlated with clinical pathological features such as tumor size, differentiation degree, capsule invasion, lymph node metastasis, etc. (all P < 0.05). The expression of miR-199b-5pmRNA and s HLA-G were negatively correlated. ROC curve identified that miR-199b-5pmRNA and HLA-g had obvious diagnostic value for thyroid cancer patients. Kaplan-Meier survival analysis manifested that the 1- and 3-year survival rates of the miR-199b-5p low expression group in thyroid cancer tissues were lower than the miR-199b-5p high expression group, and the rates of the s HLA-G low expression group were higher than the s HLA-G high expression group.

Conclusion: The miR-199b-5p expression in thyroid cancer tissues and HLA-g in serum were related to tumor size, differentiation degree, capsular invasion, lymph node metastasis and other characteristics. MiR-199b-5p may jointly affect the progression of thyroid cancer with s HLA-G.
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http://dx.doi.org/10.1016/j.yexmp.2021.104643DOI Listing
April 2021

Porcine reproductive and respiratory syndrome virus increases SOCS3 production via activation of p38/AP-1 signaling pathway to promote viral replication.

Vet Microbiol 2021 Apr 20;257:109075. Epub 2021 Apr 20.

Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China; College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China; College of Animal Medicine, Henan Agricultural University, Zhengzhou, 450002, China. Electronic address:

SOCS3 belongs to the suppressor of cytokine signaling (SOCS) family, which function as negative factors in host immune responses. Prior studies have noted the importance of SOCS family proteins in immunosuppression induced by some viruses. Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most important swine-borne viruses and has threatened the global swine industry with huge economic losses since it was first described in the 1980s. PRRSV is the etiological agent of PRRS, which causes reproductive failure and respiratory disorders. PRRSV causes immunosuppression thus establishing persistent infection. In this study, it was observed that SOCS3 was upregulated in PRRSV-infected primary porcine alveolar macrophages (PAMs) and Marc-145 cells with dose-dependent effects, which depends on virus replication. Deletion of AP-1 binding motif located in SOCS3 promoter inhibited promoter activities, which indicates that AP-1 is essential for PRRSV-induced SOCS3. This result was confirmed by experiments using AP-1 inhibitor, whose pretreatment suppressed SOCS3 mRNA and protein expression. Further research showed that p38 was crucial for PRRSV-induced SOCS3 production. Importantly, SOCS3 enhanced PRRSV replication during infection. Taken together, this study indicates that PRRSV infection induced SOCS3 expression through p38/AP-1 signaling pathway. These results revealed the molecular basis of SOCS3 upregulation and would advance further understanding of the strategy for viral immune evasion.
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http://dx.doi.org/10.1016/j.vetmic.2021.109075DOI Listing
April 2021