Publications by authors named "Li Wan"

822 Publications

Robotic-assisted choledochal cyst excision with Roux-en-Y hepaticojejunostomy in children: does age matter?

Surg Endosc 2022 Aug 4. Epub 2022 Aug 4.

Department of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Robotic-assisted surgery (RAS) is being increasingly used in pediatric choledochal cysts (CCs), but is most commonly performed in older children and adolescents. The outcomes in young infants remain to be explored. The purpose of this study is to compare outcomes in infants aged ≤ 1 year with an older cohort.

Methods: From July 2015 to January 2020, a retrospective study was conducted to evaluate the RAS in patients with CCs at our institution. Patients were divided into two groups (group A ≤ 1 year old and group B > 1 year old). Demographics, intraoperative details, complications, and outcomes were analyzed.

Results: A total of 79 patients were included in the study (28 patients in group A and 51patients in group B). The median age of patients at the surgery in group A was 4.9 months (IQR: 3.1-9.1), compared with 46.8 months (IQR: 28.5-86.5) in group B. Three patients in group A were neonates. No conversion to open surgery was required. No significant differences were found between the two groups including sex, Todani type, or diameter of the cysts. The diameter of the common hepatic duct was smaller in group A (6.0 ± 1.7 vs. 9.0 ± 3.0 mm; p < 0.001). Group A had the longer hepaticojejunostomy time [51(44-58) vs. 42(38-53) min; p = 0.013], while Group B had the longer cyst excision time [43(41-59) vs. 50(43-60) min; p = 0.005]. However, their total operative time and console time were similar. There were no statistical differences in length of hospital stay and complications between the two groups.

Conclusions: Robot-assisted cyst resection and hepaticojejunostomy are feasible and safe in infants ≤ 1 year old. Age cannot be considered an absolute contraindication for robotic surgery in patients with CCs.
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http://dx.doi.org/10.1007/s00464-022-09496-0DOI Listing
August 2022

Correction: 3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.

J Exp Clin Cancer Res 2022 Aug 3;41(1):236. Epub 2022 Aug 3.

The State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing, 100050, China.

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http://dx.doi.org/10.1186/s13046-022-02454-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347118PMC
August 2022

Systematic pan-cancer analysis identifies APOC1 as an immunological biomarker which regulates macrophage polarization and promotes tumor metastasis.

Pharmacol Res 2022 Jul 30;183:106376. Epub 2022 Jul 30.

The State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing 100050, China; Key Laboratory of Drug Target Research and Drug Screen, Institute of Materia Medica, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050, China. Electronic address:

Apolipoprotein C1 (APOC1) has been found to play an essential part in proliferation and metastasis of numerous cancers, but related mechanism has not been elucidated, especially its function and role in tumor immunity. Through systematic pan-cancer analysis, we identified that APOC1 was closely associated with the infiltration of various immune cells in multiple cancers. Besides, APOC1 was significantly co-expressed with the immune checkpoints, major histocompatibility complex (MHC) molecules, chemokines and other immune-related genes. Furthermore, single-cell sequencing analysis suggested that the vast majority of APOC1 was expressed in macrophages or tumor-associated macrophages (TAMs). Additionally, the expression of APOC1 was significantly related to the prognosis of different cancers. Since APOC1 was most significantly abnormally expressed in renal cell cancer (RCC), subsequent experiments were carried out in RCC to explore the role of APOC1 in tumor immunity. The expression of APOC1 was significantly elevated in the tumor and serum of RCC patients. Besides, APOC1 was mainly expressed in the macrophage and it was closely related to the immune cell infiltration of RCC. Co-culture with RCC cells could induce the generation of TAMs with M2 phenotype which be blocked by silencing APOC1. The expression of APOC1 was elevated in the M2 or TAMs and APOC1 promoted M2 polarization of macrophages through interacting with CD163 and CD206. Furthermore, macrophages overexpressing APOC1 promoted the metastasis of RCC cells via secreting CCL5. Together, these data indicate that APOC1 is an immunological biomarker which regulates macrophage polarization and promotes tumor metastasis.
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http://dx.doi.org/10.1016/j.phrs.2022.106376DOI Listing
July 2022

Correction: miR-29c plays a suppressive role in breast cancer by targeting the TIMP3/STAT1/FOXO1 pathway.

Clin Epigenetics 2022 Jul 29;14(1):97. Epub 2022 Jul 29.

The State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing, China.

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http://dx.doi.org/10.1186/s13148-022-01317-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338476PMC
July 2022

Persistent hiccup as one of the initial symptoms of leucine-rich glioma-inactivated-1 encephalitis: a case report.

BMC Neurol 2022 Jul 27;22(1):281. Epub 2022 Jul 27.

Department of Neurology, Baoding No.1 Central Hospital, 320 Northern Great Wall Street, Hebei Province, 071000, Baoding, China.

Background: Anti-leucine-rich glioma-inactivated 1 (LGI1) encephalitis, an autoimmune disorder, is characterized by faciobrachial dystonic seizures, epilepsy, memory deficits and altered mental status while hiccup is not commonly found in patients.

Case Presentation: A 62-year-old male was presented with slurred speech, abnormal gait, faciobrachial dystonic seizures and impaired cognition. Besides, the hiccup was one of the initial symptoms. His brain magnetic resonance images (MRI) revealed multiple lesions with left caudate nucleus, putamen, insula and left hippocampus involvement. Because a diagnosis of antibody-related limbic encephalitis was suspected, studies including an autoimmune profile were done by cell-based assays. After anti-LGI1 antibodies were detected in both cerebrospinal fluid and serology, pulse methylprednisolone and intravenous immunoglobulin were started and hence hiccups disappeared along with other symptoms.

Conclusions: Clinicians should be aware that persistent hiccups might be one of the initial manifestations of LGI1 subtype of voltage-gated potassium channel complex antibody associated autoimmune encephalitis.
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http://dx.doi.org/10.1186/s12883-022-02797-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327253PMC
July 2022

Six-Step Continuous Flow Synthesis of Diclofenac Sodium via Cascade Etherification/Smiles Rearrangement Strategy: Tackling the Issues of Batch Processing.

Chemistry 2022 Jul 26:e202202097. Epub 2022 Jul 26.

Engineering Center of Catalysis and Synthesis for Chiral Molecules, Department of Chemistry, Fudan University, Shanghai, 200433, P. R. China.

Invited for the cover of this issue are Dang Cheng, Fener Chen and co-workers at Fudan University. The image depicts six-step continuous-flow synthesis of diclofenac sodium from commercially available aniline and chloroacetic acid in a desktop microchemical plant. Read the full text of the article at 10.1002/chem.202201420.
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http://dx.doi.org/10.1002/chem.202202097DOI Listing
July 2022

Visual Analysis of Midwifery Research in China Based on Bibliometrics.

J Midwifery Womens Health 2022 Jul 23;67(4):478-487. Epub 2022 Jul 23.

Department of Obstetrics and Gynecology Nursing, West China Second University Hospital, Sichuan University/West China School of Nursing, Sichuan University.

Introduction: This study used bibliometrics to analyze the current trends, hotspots, and frontiers in Chinese midwifery research.

Methods: A search was conducted among midwifery literature published from 2010 to 2020, and bibliometric and information visualization analyses were conducted using the acquired data. A bibliometric analysis was performed on the quantity and trends in midwifery research, distribution of and cooperation among research institutions, coauthorship, distribution of disciplines, high-frequency keywords, and burst terms. Cooperation mode, research hotspots, and research frontiers were also analyzed.

Results: The findings indicate that the number of midwifery research studies have been steadily increasing in China over the last decade. During the study period, Guangdong Province, which received the most research funding, produced the highest number of published articles, followed by Beijing and Shanghai. Research institutions related to midwifery are mainly universities with a strong focus on scientific research. Contemporary topics include core competencies and birth outcomes; research frontiers, including education; competence; and evidence-based practice.

Discussion: China should accelerate in-depth midwifery research; increase opportunities for regional, institutional, and team cooperation; promote the sublimation of midwifery work from practical experience to disciplinary theory; and provide higher-quality professional services for maternal and child health. Bibliometric analyses may be used to examine midwifery research in other countries too.
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http://dx.doi.org/10.1111/jmwh.13379DOI Listing
July 2022

Self-Stirring Microcatalysts: Large-Scale, High-Throughput, and Controllable Preparation and Application.

Inorg Chem 2022 Aug 21;61(30):11757-11765. Epub 2022 Jul 21.

Key Laboratory of Jiangxi Province for Environment and Energy Catalysis, School of Chemistry and Chemical Engineering, Nanchang University, Nanchang 330031, Jiangxi, P. R. China.

Herein, we introduce a strategy to develop a kind of unprecedented microcatalyst, which owns self-stirring and catalytic performance based on pneumatic printing and magnetic field induction technology. A spindle-shaped microcatalyst based on metal-organic frameworks (MOFs) with a certain aspect ratio and size can be obtained by tuning the printing parameters and the intensity of the magnetic field. One nozzle can print 18 000 microcatalysts per hour, which provides a prerequisite for the realization of large-scale production in the industrial field. Furthermore, this strategy can be widely applied to a variety of other heterogeneous catalysts, such as mesoporous SiO, zeolite, metallic oxide, and so on. To demonstrate the superiority of the printed catalyst, the series of printed microcatalysts were evaluated by various catalytic reactions including liquid-phase hydrogenation, microdroplet dye-fading, and photocatalytic degradation in microreactor, all of which exhibited excellent catalytic performance.
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http://dx.doi.org/10.1021/acs.inorgchem.2c01444DOI Listing
August 2022

Adverse impact of a high allelic burden FLT3-ITD mutation on allogeneic hematopoietic stem cell transplantation in patients with cytogenetically normal AML.

Int J Hematol 2022 Jul 20. Epub 2022 Jul 20.

Institute of Blood and Marrow Transplantation, National Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, 215006, China.

Risks associated with the FLT3-ITD mutation in patients receiving chemotherapy alone for cytogenetic normal acute myeloid leukemia (CN-AML) depend on the allelic ratio (AR) and concomitant NPM1 mutation. Nevertheless, their prognostic ability after allogeneic hematopoietic cell transplantation (allo-HCT) remains undetermined. Moreover, previous studies have revealed that haploidentical transplantation improves outcomes of FLT3-ITD patients. To elucidate whether this alteration also impacts prognosis of myeloablative allo-HCT upon first remission, we retrospectively reviewed the prognostic ability of FLT3-ITD mutations in 205 CN-AML patients. Our analysis demonstrated that FLT3-ITD AR was closely related to pretransplant MRD and induction response. Multivariate analysis showed that high-AR FLT3-ITD, pretransplant MRD and induction response were independent risk factors for CN-AML. In addition, we presented evidence that the high-AR FLT3-ITD patient prognosis was not overcome by haploidentical transplantation, but was markedly improved by cGVHD. More importantly, among patients with negative pretransplant MRD, high-AR FLT3-ITD patients did not have increased relapse risk, compared to low-AR FLT3-ITD and wild-type FLT3 patients. Our findings will aid in accurate prognostic stratification of FLT3-ITD patients. We also recommend further targeted and coordinated approaches to sustain durable remission following induction chemotherapy and allo-HCT in this high-risk patient population.
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http://dx.doi.org/10.1007/s12185-022-03423-8DOI Listing
July 2022

Response: Appropriate timing for the removal of urinary catheter after laparoscopic surgery for benign gynecologic diseases may be still uncertain.

Int J Gynaecol Obstet 2022 Jul 15. Epub 2022 Jul 15.

Department of Anesthesiology, Jiaxing Maternity and Children Health Care Hospital, Affiliated Women and Children Hospital, Jiaxing University, Jiaxing, Zhejiang, China.

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http://dx.doi.org/10.1002/ijgo.14332DOI Listing
July 2022

High-Level Biosynthesis of 2'-Fucosyllactose by Metabolically Engineered .

J Agric Food Chem 2022 Jul 14;70(29):9017-9025. Epub 2022 Jul 14.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.

2'-Fucosyllactose (2'-FL) is the most abundant oligosaccharide in human milk. In this study, a highly efficient biosynthetic route for 2'-FL production was designed the pathway of GDP-l-fucose using engineered BL21(DE3). Specifically, plasmid-based strains with previously deleted and were further reconstructed by introducing pathway genes and α1,2-fucosyltransferase-encoding to realize 2'-FL synthesis. The 2'-FL titer was enhanced to 3.92 g/L by further introducing and . Subsequently, the additional expression cassette was chromosomally integrated into locus to strengthen fucosylation reaction and a strong constitutive promoter (P) was used to replace the original promoters of and to improve 2'-FL synthesis. The maximal 2'-FL titer reached 9.06 and 79.23 g/L in shake-flask and fed-batch cultivation, respectively. The 2'-FL productivity reached 1.45 g/L/h, showing remarkable production potential in large-scale industrial application.
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http://dx.doi.org/10.1021/acs.jafc.2c02484DOI Listing
July 2022

Development of Enzyme-Free DNA Amplifier Based on Chain Reaction Principle.

Acta Biomater 2022 Jul 8. Epub 2022 Jul 8.

School of Medicine, Nankai University, Tianjin 300071, China; Nankai University Eye Institute, Nankai University, Tianjin 300071, China. Electronic address:

Enzyme-free DNA amplifiers can amplify the signal of nucleic acid molecules. They can be applied to DNA molecular operation and nucleic acid detection. The reaction speed is the core index to evaluate DNA amplifiers. In this study, we designed a DNA amplifier based on an enzyme-free chain reaction. This DNA amplifier can release one more signal molecule in each round of reaction and trigger the next round, which significantly improved reaction speed. Moreover, because the amplifier used a stable DNA structure, the reaction can occur at room temperature. To integrate the amplifier into other DNA molecular operations, we performed the amplification reaction in a microfluidic chip module. The results showed that the amplifier can realize real-time signal feedback at a proper input molecule concentration and reach the endpoint in 40 s, even at a low relative concentration. To apply the amplifier for nucleic acid detection, we also used a conventional fluorescent polymerase chain reaction instrument for the reaction. The results showed that the amplifier specifically detected trace DNA single-stranded molecules. To solve the leakage problem of existing amplifiers, we designed a DNA molecule as the chain reaction's inhibitor, which was crucial in controlling the reaction speed and preventing leakage. STATEMENT OF SIGNIFICANCE: Traditional amplifier strategies of enzyme-free DNA amplifiers relied on a constant number of cycling molecules to catalyze the amplifier molecules' changing structure and release fluorescent signals, which lead low reaction speed. Based on an enzyme-free chain reaction, we designed a DNA amplifier which can release one more cycling molecule in each loop and trigger the next loop and significantly improve reaction speed in this study. Our analysis on microfluidic chip module and PCR instrument verifies high sensitivity and selectivity. And this strategy of DNA amplifier realizes the control of reaction and prevents leakage. We believe that this automated amplification strategy could have great applications in vivo signal detection, imaging, and signal molecule translation.
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http://dx.doi.org/10.1016/j.actbio.2022.06.047DOI Listing
July 2022

Evaluating the activity of nonsense-mediated RNA decay via Nanopore direct RNA sequencing.

Biochem Biophys Res Commun 2022 09 3;621:67-73. Epub 2022 Jul 3.

The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Beijing Hospital, National Center of Gerontology, National Health Commission, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, PR China; Graduate School of Peking Union Medical College, Beijing, 100730, PR China; Clinical Biobank, Beijing Hospital, National Center of Gerontology, National Health Commission, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, PR China. Electronic address:

Nonsense-mediated mRNA decay (NMD) and its regulation play an important role in eliminating faulty transcripts and controlling gene expression. However, measuring NMD activity and characterizing its targets remain challenging. In this study, we set out to establish Nanopore direct RNA sequencing in combination with quantitative real-time PCR (qPCR) as a method for analyzing NMD activity and its targets in cultured cell lines and clinical tissue samples. Nanopore RNA sequencing could detect more isoforms than short-read sequencing, especially in identifying novel isoforms and predicting isoforms annotated with premature termination codon (PTC). Changes in transcriptional isoforms of five genes (PRS, RPL12, SRSF2, PPIA, and TMEM208) could faithfully reflect NMD activity in the three cell lines and prostate cancer (PCA) samples. NMD activity in PCA samples varied, but some patients showed an increased trend. Together, Nanopore sequencing was superior in identifying NMD targets and evaluating NMD activity compared with short-read sequencing, and the NMD markers we screened may be used for measuring NMD activity in clinical patients.
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http://dx.doi.org/10.1016/j.bbrc.2022.06.055DOI Listing
September 2022

Agreement of infrared ear temperature with nasopharyngeal temperature and diagnostic performance on hypothermia in general anesthetized patients.

J Chin Med Assoc 2022 Jul 6. Epub 2022 Jul 6.

Department of Anesthesiology, Jiaxing Maternity and Children Health Care Hospital, Affiliated Women and Children Hospital, Jiaxing University, Jiaxing, Zhejiang, China.

Background: Infrared ear thermometry is widely used in clinical practice due to its noninvasive, convenient, and quick sampling. However, its accuracy and feasibility in anesthetized patients have not yet been established.

Methods: We conducted this cross-sectional study to evaluate the agreement between infrared ear temperature and nasopharyngeal temperature in general anesthetized patients and its performance in intraoperative hypothermia, defined as nasopharyngeal temperature <36°C. Adult female patients who underwent gynecological surgery under general anesthesia were enrolled in this study. Infrared ear temperature by Braun ThermoScan PRO 4000 and nasopharyngeal temperature were measured simultaneously before, during, and after surgery. The agreement between the two temperatures was assessed using the intraclass correlation coefficient (ICC) and Bland-Altman analysis. The diagnostic performance of the infrared ear thermometer for hypothermia was evaluated using receiver operating characteristic (ROC) curve analysis.

Results: Fifty-six patients with 168 pairs of simultaneous infrared ear and nasopharyngeal temperatures were included in this analysis. The mean infrared ear temperature was consistently higher than the nasopharyngeal temperature throughout surgery, but the differences were small (0.22°C, 0.13°C, and 0.06°C before, during, and after surgery, respectively). The ICC between the two temperatures before, during, and after surgery was 0.70, 0.75, and 0.80, respectively, and 93.5% of the differences fell within the 95% limits of agreement of ± 0.5°C. An infrared ear thermometer had high diagnostic accuracy for hypothermia, with an area under the ROC curve of 0.95 (95% confidence interval [CI], 0.92-0.98). The cut-off of infrared ear temperature for hypothermia was 36.2°C with a sensitivity of 0.89 (95% CI, 0.71-0.98) and a specificity of 0.87 (95% CI, 0.81-0.92).

Conclusion: The infrared ear temperature is in good agreement with the nasopharyngeal temperature in general anesthetized patients without hyperthermia and has high performance for detecting hypothermia. An infrared ear thermometer can be a diagnostic tool for intraoperative hypothermia.
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http://dx.doi.org/10.1097/JCMA.0000000000000770DOI Listing
July 2022

Erythrocyte Membrane-Coated Invisible Acoustic-Sensitive Nanoparticle for Inducing Tumor Thrombotic Infarction by Precisely Damaging Tumor Vascular Endothelium.

Small 2022 Jul 5;18(30):e2201933. Epub 2022 Jul 5.

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.

Selective induction of tumor thrombus infarction is a promising antitumor strategy. Non-persistent embolism due to non-compacted thrombus and activated fibrinolytic system within the tumor large blood vessels and tumor margin recurrence are the main therapeutic bottlenecks. Herein, an erythrocyte membrane-coated invisible acoustic-sensitive nanoparticle (TXA+DOX/PFH/[email protected]) is described, which can induce tumor thrombus infarction by precisely damaging tumor vascular endothelium. It is revealed that TXA+DOX/PFH/[email protected] can effectively accumulate on the endothelial surface of tumor vessels with the help of the red blood cell membrane (RBCM) stealth coating and RGD cyclic peptide (cRGD), which can be delivered in a targeted manner as nanoparticle missiles. As a kind of phase-change material, perfluorohexane (PFH) nanodroplets possess excellent acoustic responsiveness. Acoustic-sensitive missiles can undergo an acoustic phase transition and intense cavitation with response to low-intensity focused ultrasound (LIFU), damaging the tumor vascular endothelium, rapidly initiating the coagulation cascade, and forming thromboembolism in the tumor vessels. The drugs loaded in the inner water phase are released explosively. Tranexamic acid (TXA) inhibits the fibrinolytic system, and doxorubicin (DOX) eliminates the margin survival. In summary, a stealthy and acoustically responsive multifunctional nanoparticle delivery platform is successfully developed for inducing thrombus infarction by precisely damaging tumor vascular endothelium.
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http://dx.doi.org/10.1002/smll.202201933DOI Listing
July 2022

Pharmacological manipulation of Ezh2 with salvianolic acid B results in tumor vascular normalization and synergizes with cisplatin and T cell-mediated immunotherapy.

Pharmacol Res 2022 Aug 30;182:106333. Epub 2022 Jun 30.

Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Joint International Research Laboratory of Chinese Medicine and Regenerative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine (TCM) Prevention and Treatment of Tumor, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address:

Tumor vasculature is characterized by aberrant structure and function, resulting in immune suppressive profiles of tumor microenvironment (TME) through limiting immune cell infiltration into tumors. The defective vascular perfusion in tumors also impairs the delivery and efficacy of chemotherapeutic agents. Targeting abnormal tumor blood vessels has emerged as an effective therapeutic strategy to improve the outcome of chemotherapy and immunotherapy. In this study, we demonstrated that Salvianolic acid B (SalB), one of the major ingredients of Salvia miltiorriza elicited vascular normalization in the mouse models of breast cancer, contributing to improved delivery and response of chemotherapeutic agent cisplatin as well as attenuated metastasis. Moreover, SalB in combination with anti-PD-L1 blockade retarded tumor growth, which was mainly due to elevated infiltration of immune effector cells and boosted delivery of anti-PD-L1 into tumors. Mechanistically, tumor cell enhancer of zeste homolog 2 (Ezh2)-driven cytokines disrupted the endothelial junctions with diminished VE-cadherin expression, which could be rescued in the presence of SalB. The restored vascular integrity by SalB via modulating the interactions between tumor cells and endothelial cells (ECs) offered a principal route for achieving vascular normalization. Taken together, our data elucidated that SalB enhanced sensitivity of tumor cells to chemotherapy and immunotherapy through triggering tumor vascular normalization, providing a potential therapeutic strategy of combining SalB and chemotherapy or immunotherapy for patients with breast cancer.
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http://dx.doi.org/10.1016/j.phrs.2022.106333DOI Listing
August 2022

Laparoscopic Treatment Strategies for Liver Echinococcosis.

Infect Dis Ther 2022 Aug 25;11(4):1415-1426. Epub 2022 Jun 25.

Department of General Surgery, The General Hospital of Western Theater command, Chengdu, 610083, People's Republic of China.

Liver echinococcosis is clinically mainly composed of hepatic cystic echinococcosis (CE) and hepatic alveolar echinococcosis (AE). At present, laparoscopy has been comprehensively applied in the treatment of two types of liver echinococcosis. For hepatic CE treatment, both laparoscopic total pericystectomy and laparoscopic hepatectomy can achieve radical results, but the former is considered the first choice owing to its being more minimally invasive; laparoscopic subtotal pericystectomy and laparoscopic partial pericystectomy can be accepted as complementary options, considering the presence of complicated cysts and the level of laparoscopic technique in remote hospitals; laparoscopic cystectomy is simple, but it is not currently recommended for treatment of hepatic CE owing to poor efficacy and high risk of postoperative complications. For hepatic AE treatment, laparoscopy not only achieves the same radical effect as open surgery in selected patients, but also is more minimally invasive, so it has a better prospect.
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http://dx.doi.org/10.1007/s40121-022-00664-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334471PMC
August 2022

Furosemide prevents membrane KCC2 downregulation during convulsant stimulation in the hippocampus.

IBRO Neurosci Rep 2022 Jun 28;12:355-365. Epub 2022 Apr 28.

Department of Neurology, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institute of Biological Science, Zhongshan Hospital, Fudan University, Shanghai 200032, China.

In adults, γ-aminobutyric acid (GABA) type A receptor (GABAR)-mediated inhibition depends on the maintenance of low intracellular chloride anion concentration through neuron-specific potassium-chloride cotransporter-2 (KCC2). KCC2 has been widely reported to have a plasticity change during the course of epilepsy development, with an early downregulation and late recovery in neuronal cell membranes after epileptic stimulation, which facilitates epileptiform burst activity. Furosemide is a clinical loop diuretic that inhibits KCC2. Here, we first confirmed that furosemide pretreatment could effectively prevented convulsant stimulation-induced neuronal membrane KCC2 downregulation in the hippocampus in both in vivo and in vitro cyclothiazide-induced seizure model. Second, we verified that furosemide pretreatment rescued KCC2 function deficits, as indicated by depolarizing shift and GABAR inhibitory function impairment induced via cyclothiazide treatment. Further, we demonstrated that furosemide also suppressed cyclothiazide-induced epileptiform burst activity in cultured hippocampal neurons and lowered the mortality rate during acute seizure induction. Overall, furosemide prevents membrane KCC2 downregulation during acute seizure induction, restores KCC2-mediated GABA inhibition, and interrupts the progression from acute seizure to epileptogenesis.
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http://dx.doi.org/10.1016/j.ibneur.2022.04.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210493PMC
June 2022

Oxalierpenes A and B, Unusual Indole-Diterpenoid Derivatives with Antiviral Activity from a Marine-Derived Strain of the Fungus .

J Nat Prod 2022 Jul 21;85(7):1880-1885. Epub 2022 Jun 21.

College of Pharmaceutical Sciences, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnostics of Education Ministry of China, Hebei University, Baoding 071002, People's Republic of China.

Oxalierpenes A and B ( and ), two unusual indole-diterpenoid derivatives, were obtained from the marine-derived fungus . The absolute configurations of and were elucidated by calculated TDDFT ECD and DP4plus methods. Oxalierpene A () represents the first indole-diterpenoid derivative with a five-membered ring of 4-hydroxy-5,5-dimethyldihydrofuran-3-one as a side chain. Oxalierpene B () has a unique 6/5/6/5/5/6/6/5/5 ring system. Compounds and showed antiviral activity against the H1N1 virus and respiratory syncytial virus (RSV), with IC values ranging from 2.8 to 9.4 μM.
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http://dx.doi.org/10.1021/acs.jnatprod.2c00322DOI Listing
July 2022

Effect of Normobaric Oxygen Inhalation Intervention on Microcirculatory Blood Flow and Fatigue Elimination of College Students After Exercise.

Front Genet 2022 1;13:901862. Epub 2022 Jun 1.

School of Physical Education, Hubei Minzu University, Enshi, China.

To explore the effect of normobaric oxygen inhalation intervention on microcirculatory blood flow of college students after exercise and the impact of the elimination of exercise-induced fatigue, to provide a theoretical and methodological reference for the rapid elimination of fatigue of college students after endurance exercise. Forty-eight male non-sports majors of Hubei University for nationalities were randomly divided into the control group (n = 24) and intervention group (n = 24). The subjects in both groups completed the same exercise program twice (running 3,000 m on the treadmill at maximum speed). After running, the issues in the intervention group inhaled portable oxygen for 30 min, and the control group recovered naturally. Microcirculatory blood flow (MBP), blood flow velocity (AVBC), blood flow concentration (CMBC), muscle oxygen saturation (SmO2), heart rate (HR), blood lactic acid (BLA), blood urea (BU), and creatine kinase (CK) were measured before exercise, immediately after exercise and 30 min after exercise. 1) MBP and AVBC had interaction between groups and time before and after exercise, MBP and AVBC immediately after exercise in the intervention group were significantly higher than those before exercise and 30 min after exercise, and 30 min after exercise in the intervention group were significantly higher than those in the control group. 2) SmO2, HR, BLA, BU, and CK had interaction between groups and time, and SmO2 immediately after exercise in the intervention group was significantly lower than that before exercise and 30 min after exercise, but significantly higher than that in the control group at 30 min after exercise. The HR and BLA immediately after exercise in the intervention group were significantly higher than those before exercise and 30 min after exercise, but significantly lower than those in the control group at 30 min after exercise, and the BU and CK in the intervention group were significantly higher than those before exercise, but significantly lower than those in the control group at 30 min after exercise. Normobaric oxygen inhalation for 30 min after exercise can delay the decrease of microcirculatory blood flow, increase muscle oxygen saturation, and promote the recovery of heart rate, blood lactic acid, blood urea and creatine kinase. Therefore, normobaric oxygen inhalation for 30 min after exercise can be used as an effective means to promote the elimination of exercise-induced fatigue after endurance running.
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http://dx.doi.org/10.3389/fgene.2022.901862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198422PMC
June 2022

The Impact of China's Paired Assistance Policy on the COVID-19 Crisis-An Empirical Case Study of Hubei Province.

Front Public Health 2022 30;10:885852. Epub 2022 May 30.

Department of Applied Economics, School of Applied Economics, Renmin University of China, Beijing, China.

To control the coronavirus pandemic (COVID-19), China implemented the Paired Assistance Policy (PAP). Local responders in 16 cities in Hubei Province were paired with expert teams from 19 provinces and municipalities. Fully supported by the country's top-down political system, PAP played a significant role in alleviating the COVID-19 pandemic in Hubei Province and China as a whole. In this study, we examined PAP using a two-way fixed effects model with the cumulative number of medical support personnel and cumulative duration as measurements. The results show personnel and material support played an active role in the nation's response to the COVID-19 public health crisis.
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http://dx.doi.org/10.3389/fpubh.2022.885852DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196880PMC
June 2022

Chemical analysis of Chrysosplenium from different species by UPLC-Q exactive orbitrap HRMS and HPLC-DAD.

J Pharm Biomed Anal 2022 Sep 1;218:114861. Epub 2022 Jun 1.

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, Sichuan, China. Electronic address:

Chrysosplenium is the main component of a variety of Tibetan prescription preparations. Nevertheless, there are few chemical reports for different species of Chrysosplenium, which should be further explored. To this end, ultra-performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q Exactive Orbitrap HRMS) and high-performance liquid chromatography-diode array detection (HPLC-DAD) were first integrated to qualitatively analyse the chemical characteristics of Chrysosplenium nudicaule, Chrysosplenium carnosum, Chrysosplenium sikangense, Chrysosplenium griffithii, Chrysosplenium absconditicapsulum, Chrysosplenium forrestii and Chrysosplenium axillare. As a result, a total of 40 compounds were identified or tentatively identified from these 7 species of Chrysosplenium, including 21 flavonoids, 3 triterpenoids and a variety of alkaloids, organic acids and anthraquinones, etc. Among them, 6 compounds were detected for the first time, and 8 compounds are common components in all 7 species of Chrysosplenium. In the specific chromatogram, 4 characteristic peaks, namely Riboflavin, 5,4'-dihydroxy-3,6,3'-trimethoxyflavin-7-O-β-D-glucoside, 5,7,3'-trihydroxy-6,4',5'-trimethoxyflavone and Chrysosplenetin, were selected to evaluate the similarities of 17 batches of Chrysosplenium samples, which ranged from 0.770 to 0.994. The established method is simple, feasible and accurate, and was proven to be suitable for characterizing the chemical compositions of Chrysosplenium from different species and evaluating their similarities by specific chromatogram analysis to clarify the rationality of using Chrysosplenium from different species in clinical medication, which provides experimental data for further quality evaluation of Chrysosplenium.
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http://dx.doi.org/10.1016/j.jpba.2022.114861DOI Listing
September 2022

IL-39 promotes chronic graft-versus-host disease by increasing T and B Cell pathogenicity.

Exp Hematol Oncol 2022 Jun 2;11(1):34. Epub 2022 Jun 2.

National Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, 215006, China.

Background: Chronic graft-versus-host disease (cGVHD) remains a major complication during the late phase of allogeneic hematopoietic stem cell transplantation (allo-HSCT). IL-39, a newly described pro-inflammatory cytokine belonging to the IL-12 family, plays a role in lupus development. Recently, IL-39 has been identified as a pathogenic factor in acute GVHD (aGVHD). However, the role of IL-39 in the pathogenesis of cGVHD remains unclear.

Methods: We constructed a recombinant IL-39 plasmid and established scleroderma and lupus-like cGVHD models. Quantitative PCR and enzyme-linked immunosorbent assay (ELISA) were used to detect IL-39 expression in mice and patients post transplantation, respectively. Hydrodynamic gene transfer (HGT) was performed to achieve IL-39 overexpression in vivo. Multiparameter flow cytometry, western blotting, and assays in vitro were performed to investigate the effect of IL-39 on cGVHD.

Results: The relative expression of IL-23p19 and EBi3 was significantly increased in the intestine of cGVHD mice on day 40 post allo-HSCT, and IL-39 levels were significantly elevated in the serum of patients following allo-HSCT. Overexpression of IL-39 significantly aggravated the severity of cGVHD. Increased IL-39 levels promoted T-cell activation and germinal center responses, and may exacerbate thymic damage. Consistently, blocking IL-39 markedly ameliorated immune dysregulation in the cGVHD mice. Furthermore, we found that IL-39 was produced by B cells, CD11b cells, and CD8T cells after activation. Stimulation of IL-39 led to upregulation of the IL-39 receptor on CD4T cells and further caused activation of the STAT1/STAT3 pathway, through which IL-39 may exert its pro-inflammatory effects.

Conclusion: Our study reveals a critical role for IL-39 in cGVHD pathogenesis and indicates that IL-39 may serve as a potential therapeutic target for cGVHD prevention.
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http://dx.doi.org/10.1186/s40164-022-00286-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161463PMC
June 2022

Immune Landscape and Classification in Lung Adenocarcinoma Based on a Novel Cell Cycle Checkpoints Related Signature for Predicting Prognosis and Therapeutic Response.

Front Genet 2022 11;13:908104. Epub 2022 May 11.

Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China.

Lung adenocarcinoma (LUAD) is one of the most common malignancies with the highest mortality globally, and it has a poor prognosis. Cell cycle checkpoints play a central role in the entire system of monitoring cell cycle processes, by regulating the signalling pathway of the cell cycle. Cell cycle checkpoints related genes (CCCRGs) have potential utility in predicting survival, and response to immunotherapies and chemotherapies. To examine this, based on CCCRGs, we identified two lung adenocarcinoma subtypes, called cluster1 and cluster2, by consensus clustering. Enrichment analysis revealed significant discrepancies between the two subtypes in gene sets associated with cell cycle activation and tumor progression. In addition, based on Least Absolute Shrinkage and Selection Operator (LASSO) Cox regression, we have developed and validated a cell cycle checkpoints-related risk signature to predict prognosis, tumour immune microenvironment: (TIME), immunotherapy and chemotherapy responses for lung adenocarcinoma patients. Results from calibration plot, decision curve analysis (DCA), and time-dependent receiver operating characteristic curve (ROC) revealed that combining age, gender, pathological stages, and risk score in lung adenocarcinoma patients allowed for a more accurate and predictive nomogram. The area under curve for lung adenocarcinoma patients with 1-, 3-, 5-, and 10-year overall survival was: 0.74, 0.73, 0.75, and 0.81, respectively. Taken together, our proposed 4-CCCRG signature can serve as a clinically useful indicator to help predict patients outcomes, and could provide important guidance for immunotherapies and chemotherapies decision for lung adenocarcinoma patients.
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http://dx.doi.org/10.3389/fgene.2022.908104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130860PMC
May 2022

Six-Step Continuous Flow Synthesis of Diclofenac Sodium via Cascade Etherification/Smiles Rearrangement Strategy: Tackling the Issues of Batch Processing.

Chemistry 2022 May 31:e202201420. Epub 2022 May 31.

Engineering Center of Catalysis and Synthesis for Chiral Molecules, Department of Chemistry, Fudan University, Shanghai, 200433, P. R. China.

Diclofenac sodium is a widely used nonsteroidal anti-inflammatory drug (NSAID) as over-the-counter (OTC) medication for the treatment of inflammatory diseases. Herein, the development of an intensified six-step continuous flow synthesis of diclofenac sodium from commercially available aniline and chloroacetic acid is described. A challenging and unprecedented etherification/Smiles rearrangement cascade of 2-chloro-N-phenylacetamide and 2,6-dichlorophenol into hydroxyacetyldiphenylamine operated with the precise control of reaction conditions in continuous flow was realized as the key step in this multistep synthetic chemistry. The undesired amide hydrolysis in Smiles rearrangement was addressed and the extra installation of N-chloroacetyl group in current industrial batch mode was avoided. Diclofenac sodium was obtained in 63 % isolated yield with an average yield of above 90 % for each step in a total residence time of 205 min.
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http://dx.doi.org/10.1002/chem.202201420DOI Listing
May 2022

Feasibility of immediate removal of urinary catheter after laparoscopic gynecological surgery for benign diseases: A meta-analysis of randomized controlled trials.

Int J Gynaecol Obstet 2022 May 26. Epub 2022 May 26.

Department of Anesthesiology, Jiaxing Maternity and Children Health Care Hospital, Affiliated Women and Children Hospital, Jiaxing University, Jiaxing, Zhejiang, China.

Background: The proper time for removing the urinary catheter after gynecologic laparoscopy is unclear.

Objectives: To assess the feasibility of immediate catheter removal after benign gynecologic laparoscopy.

Search Strategy: PubMed, Embase, Web of Science, Cochrane Central Register of Controlled Trials, and Wanfang Data were searched from inception to November 30, 2021.

Selection Criteria: Only randomized controlled trials published in English or Chinese comparing immediate versus delayed catheter removal after gynecologic laparoscopy for benign diseases were included.

Data Collection And Analysis: The primary outcome was the incidence of postoperative urinary retention (PUR). A random effects model was used to calculate pooled relative risk (RR) and 95% confidence interval (CI).

Main Results: Six studies were included in this meta-analysis. There was no significant difference in PUR between immediate and delayed catheter removal (RR 1.51, 95% CI 0.37-6.18), but the evidence was of very low quality. Subgroup analysis according to the type of surgery showed a higher rate of PUR with immediate removal after hysterectomy than after other surgeries. Immediate removal was associated a lower incidence of urinary tract infection and a shorter time to mobilization compared with delayed removal.

Conclusions: Immediate removal of the urinary catheter is feasible and beneficial after benign gynecologic laparoscopy.
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http://dx.doi.org/10.1002/ijgo.14283DOI Listing
May 2022

Hydroxy-Tetraphenylimidazole Derivatives as Efficient Blue Emissive Materials for Electroluminescent Devices.

Chem Asian J 2022 Jul 24;17(14):e202200266. Epub 2022 May 24.

Department of Materials Science and Engineering, School of Molecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology, 21210, Wangchan, Rayong, Thailand.

Herein, three hydroxy-tetraphenylimidazole (HPI)-based fluorophores (HPI-TPA, HPI-PCz, and HPI-CzP) are designed and synthesized by disubstituted HPI core with arylamine units of triphenylamine (TPA), phenyl carbazole (PCz), and carbazole phenyl (CzP) at 3,5-positions of the N-phenyl ring of HPI, respectively. Their photophysical properties are theoretically and experimentally examined. HPI-TPA shows a hybridized local and charge transfer (HLCT) excited state characteristic and emits deep blue color via an HLCT mechanism, while both HPI-PCz and HPI-CzP exhibit excited-state intramolecular proton transfer (ESIPT) property and display pure keto form emissions. They possess high thermal stability and are successfully fabricated as emitters in organic light-emitting diodes (OLEDs). All devices exhibit intense blue color emissions with low turn-on voltages (3.5-3.7 V). Particularly, HPI-TPA-based OLED emits light in the deep-blue region with a high maximum external quantum efficiency (EQE ) of 3.77% and a decent efficiency roll-off.
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http://dx.doi.org/10.1002/asia.202200266DOI Listing
July 2022

Heavy metal and nutrient concentrations in top- and sub-soils of greenhouses and arable fields in East China - Effects of cultivation years, management, and shelter.

Environ Pollut 2022 Aug 18;307:119494. Epub 2022 May 18.

Institute of Meteorology and Climate Research, Atmospheric Environmental Research (IMK-IFU), Karlsruhe Institute of Technology, Garmisch-Partenkirchen, 82467, Germany; State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry, Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing, 100029, China. Electronic address:

Although greenhouse vegetable production in China is rapidly changing, consumers are concerned about food quality and safety. Studies have shown that greenhouse soils are highly eutrophicated and potentially contaminated by heavy metals. However, to date, no regional study has assessed whether greenhouse soils differ significantly in their heavy metal and nutrient loads compared to adjacent arable land. Our study was conducted in Shouguang County, a key region of greenhouse vegetable production in China. Soil samples down to soil depths of 3 m were taken from 60 greenhouse vegetable fields of three different ages (5, 10, and 20 years) and from 20 adjacent arable fields to analyze the concentrations of heavy metals, nutrients, and soil physio-chemical parameters. A comparison of greenhouse soils with adjacent arable fields revealed that for greenhouses, (a) micro (heavy metals: Cu, Zn, and Mn) and macronutrients (Nmin, Olsen-P, available K) were significantly higher by a factor of about five, (b) N:P:K ratios were significantly imbalanced towards P and K, and (c) topsoil (0-30 cm) concentrations of the above-mentioned micro- and macronutrients increased with years of vegetable cultivation. In contrast, the soil concentrations of the heavy metals Cr and Pb were lower in greenhouse soils. Heavy metal concentrations did not vary significantly with soil depth, except for the micronutrients Cu and Zn, which were between 1- and 3-fold higher in the topsoil (0-30 cm) than in the subsoil (30-300 cm). The Nemerow pollution index (P) was 0.37, which was below the recommended environmental threshold value (P < 1). Structural equation model analysis revealed that soil nutrient concentrations in greenhouse soils are directly related to the input of fertilizers and agrochemicals. Lower values of soil Pb and Cr concentrations in greenhouses were due to the sheltering effect of the greenhouse roof, which protected soils from atmospheric deposition due to emissions from nearby industrial complexes.
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http://dx.doi.org/10.1016/j.envpol.2022.119494DOI Listing
August 2022
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