Publications by authors named "Li Song"

2,639 Publications

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Management of type IIIb dens invaginatus using a combination of root canal treatment, intentional replantation, and surgical therapy: A case report.

World J Clin Cases 2022 Jun;10(18):6261-6268

Stomatological Hospital and College, Key Laboratory of Oral Diseases Research of Anhui Province, Anhui Medical University, Hefei 230032, Anhui Province, China.

Background: Type Ⅲb dens invaginatus (DI) with a lateral canal located at the mid-third of the root is rarely reported. Here, we report a rare case of type Ⅲb DI in the left upper anterior tooth with a lateral canal that led to persistent periodontitis.

Case Summary: A 15-year-old female patient presented with a chief complaint of pain associated with recurrent labial swelling in the area of the left anterior tooth. A diagnosis of type Ⅲb DI and chronic periodontitis was made. Intentional replantation was performed after conventional endodontic treatment failed. After 6 mo, the patient was asymptomatic, but a sinus tract was observed. Cone-beam computed tomography images showed bone loss in the mesial of the mid-root. Based on methylene blue staining and microscopy images, the lateral foramen located at the middle third of the root was surgically treated. After 3 years of follow-up, the clinical findings and radiographic assessment presented a favorable prognosis of bone healing without root absorption or ankylosis.

Conclusion: Type Ⅲb DI with a lateral canal can be successfully treated by root canal treatment, intentional replantation, and surgical therapy.
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http://dx.doi.org/10.12998/wjcc.v10.i18.6261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254211PMC
June 2022

Oncolytic Impediment/Promotion Balance Disruption by Sonosensitizer-Free Nanoplatforms Unfreezes Autophagy-Induced Resistance to Sonocatalytic Therapy.

ACS Appl Mater Interfaces 2022 Aug 8. Epub 2022 Aug 8.

Central Laboratory, Department of Stomatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, No. 301 Yan-chang-zhong Road, Shanghai 200072, P. R. China.

Autophagy as a double-edged sword features an oncolytic impediment/promotion balance, which manipulates tumor progression. From this perspective, a sonosensitizer-free targeting oncolytic nanoplatform (SFTON) consisting of chloroquine (CQ) and porphyrin-structured metal centers (PMCS) was engineered to break this balance for enhancing antitumor activity. Porphyrin structure retention in a ZIF-8-derived hydrophobic carbon skeleton retained high stability and high sonocatalytic activity, and the hydrophobic carbon skeleton capable of adsorbing air provided cavitation nuclei for further elevating sonocatalytic activity. More significantly, the encapsulated CQ as the autophagy inhibitor reprogrammed autophagy, terminated the autophagy-induced self-protection or self-detoxification, and unfroze the resistances to reactive oxygen species (ROS) therapy associated with ROS accumulation and ROS activity. Systematic experiments reveal the action principles and validate that the induced apoptosis and blockaded autophagosome escalation into the autolysosome were two activated pathways to magnify the antitumor sonocatalytic therapy. Contributed by these actions, the SFTON-unlocked oncolytic impediment/promotion balance disruption strategy acquired considerable antitumor outcomes in vivo and in vitro against liver tumor progression, especially after combining with AS1411-mediated active targeting. This impediment/promotion balance disruption enabled by the SFTON can serve as a general method to elevate ROS-based antitumor activity.
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http://dx.doi.org/10.1021/acsami.2c09443DOI Listing
August 2022

Aging Relevant Metabolite Itaconate Inhibits Inflammatory Bone Loss.

Front Endocrinol (Lausanne) 2022 22;13:885879. Epub 2022 Jul 22.

Department of Orthopaedic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Progressive bone loss during aging makes osteoporosis one of the most common and life impacting conditions in geriatric populations. The bone homeostasis is maintained through persistent remodeling mediated by bone-forming osteoblast and bone-resorbing osteoclast. Inflammaging, a condition characterized by increased pro-inflammatory markers in the blood and other tissues during aging, has been reported to be associated with skeletal stem/progenitor cell dysfunction, which will result in impaired bone formation. However, the role of age-related inflammation and metabolites in regulation of osteoclast remains largely unknown. In the present study, we observed dichotomous phenotypes of anti-inflammatory metabolite itaconate in responding to inflammaging. Itaconate is upregulated in macrophages during aging but has less reactivity in responding to RANKL stimulation in aged macrophages. We confirmed the inhibitory effect of itaconate in regulating osteoclast differentiation and activation, and further verified the rescue role of itaconate in lipopolysaccharides induced inflammatory bone loss animal model. Our findings revealed that itaconate is a crucial regulatory metabolite during inflammaging that inhibits osteoclast to maintain bone homeostasis.
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http://dx.doi.org/10.3389/fendo.2022.885879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353012PMC
August 2022

Overexpression of confers drought and salinity tolerance by enhancing root system and antioxidation ability in Arabidopsis.

Front Plant Sci 2022 22;13:967352. Epub 2022 Jul 22.

Department of Plant Sciences, University of California, Davis, Davis, CA, United States.

Myrothamnus flabellifolia is the only woody resurrection plant discovered so far and could recover from extreme desiccation condition. However, few genes related to its strong drought tolerance have been characterized, and the underlying molecular mechanisms remains mysterious. Members of WRKY transcription factor family are effective in regulating abiotic stress responses or tolerance in various plants. An early dehydration-induced gene encoding a WRKY transcription factor namely MfWRKY41 was isolated from , which is homologous to AtWRKY41 of Arabidopsis. It contains a typical WRKY domain and zinc finger motif, and is located in the nucleus. Comparing to wild type, the four transgenic lines overexpressing showed better growth performance under drought and salt treatments, and exhibited higher chlorophyll content, lower water loss rate and stomatal aperture and better osmotic adjustment capacity. These results indicated that MfWRKY41 of positively regulates drought as well as salinity responses. Interestingly, the root system architecture, including lateral root number and primary root length, of the transgenic lines was enhanced by MfWRKY41 under both normal and stressful conditions, and the antioxidation ability was also significantly improved. Therefore, MfWRKY41 may have potential application values in genetic improvement of plant tolerance to drought and salinity stresses. The molecular mechanism involving in the regulatory roles of MfWRKY41 is worthy being explored in the future.
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http://dx.doi.org/10.3389/fpls.2022.967352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355591PMC
July 2022

Inhibition of Rac1 in ventral hippocampal excitatory neurons improves social recognition memory and synaptic plasticity.

Front Aging Neurosci 2022 22;14:914491. Epub 2022 Jul 22.

Program in Neurosciences and Mental Health, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning, Toronto, ON, Canada.

Rac1 is critically involved in the regulation of the actin cytoskeleton, neuronal structure, synaptic plasticity, and memory. Rac1 overactivation is reported in human patients and animal models of Alzheimer's disease (AD) and contributes to their spatial memory deficits, but whether Rac1 dysregulation is also important in other forms of memory deficits is unknown. In addition, the cell types and synaptic mechanisms involved remain unclear. In this study, we used local injections of AAV virus containing a dominant-negative (DN) Rac1 under the control of CaMKIIα promoter and found that the reduction of Rac1 hyperactivity in ventral hippocampal excitatory neurons improves social recognition memory in APP/PS1 mice. Expression of DN Rac1 also improves long-term potentiation, a key synaptic mechanism for memory formation. Our results suggest that overactivation of Rac1 in hippocampal excitatory neurons contributes to social memory deficits in APP/PS1 mice and that manipulating Rac1 activity may provide a potential therapeutic strategy to treat social deficits in AD.
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http://dx.doi.org/10.3389/fnagi.2022.914491DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354987PMC
July 2022

NiPS ultrathin nanosheets as versatile platform advancing highly active photocatalytic H production.

Nat Commun 2022 Aug 6;13(1):4600. Epub 2022 Aug 6.

School of Chemical Engineering and Advanced Materials, University of Adelaide, Adelaide, SA 5005, Australia.

High-performance and low-cost photocatalysts play the key role in achieving the large-scale solar hydrogen production. In this work, we report a liquid-exfoliation approach to prepare NiPS ultrathin nanosheets as a versatile platform to greatly improve the light-induced hydrogen production on various photocatalysts, including TiO, CdS, InZnS and CN. The superb visible-light-induced hydrogen production rate (13,600 μmol h g) is achieved on NiPS/CdS hetero-junction with the highest improvement factor (~1,667%) compared with that of pure CdS. This significantly better performance is attributed to the strongly correlated NiPS/CdS interface assuring efficient electron-hole dissociation/transport, as well as abundant atomic-level edge P/S sites and activated basal S sites on NiPS ultrathin nanosheets advancing hydrogen evolution. These findings are revealed by the state-of-art characterizations and theoretical computations. Our work for the first time demonstrates the great potential of metal phosphorous chalcogenide as a general platform to tremendously raise the performance of different photocatalysts.
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http://dx.doi.org/10.1038/s41467-022-32256-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357043PMC
August 2022

The prognostic value of the ratio of standard uptake value of lymph node to primary tumor before treatment of locally advanced nasopharyngeal carcinoma.

Eur Arch Otorhinolaryngol 2022 Aug 6. Epub 2022 Aug 6.

Department of Radiotherapy, Meizhou People's Hospital, Meizhou Academy of Medical Sciences Meizhou, Meizhou, China.

Background: To evaluate the prognostic value of the ratio of the standard uptake value of the lymph node and primary tumor before the treatment of locally advanced nasopharyngeal carcinoma and examine the prognostic value of the tumor metabolic parameters (SUVmax MTV, and TLG) of the lymph node and primary tumor of locally advanced nasopharyngeal carcinoma.

Methods: A total of 180 patients with locally advanced nasopharyngeal carcinoma diagnosed pathologically from January 1, 2016 to December 31, 2018 were selected, and the MEDEX system was used to automatically delineate the SUVmax, MTV, and TLG of the lymph node metastases and nasopharyngeal carcinoma primary tumor. In addition, the ratio of LN-SUVmax (SUVmax of the lymph node metastases) to T-SUVmax (SUVmax of the nasopharyngeal carcinoma primary tumor) was calculated, and a ROC curve was drawn to obtain the best cut-off value. Kaplan-Meier and Cox regression models were used for survival and multivariate analyses, respectively.

Results: The median follow-up period for participants was 32 (4-62) months. Univariate analysis showed that age (P = 0.013), LN-SUVmax (P = 0.001), LN-TLG (P = 0.007) and NTR (P = 0.001) were factors influencing the overall survival (OS). Factors affecting local progression-free survival (LPFS) were LN-SUVmax (P = 0.005), LN-TLG (P = 0.003) and NTR (P = 0.020), while clinical stage (P = 0.023), LN-SUVmax (P = 0.007), LN-TLG (P = 0.006), and NTR (P = 0.032) were factors affecting distant metastasis-free survival (DMFS). Multivariate analysis showed that NTR was an independent influencing factor of OS (HR 3.00, 95% CI 1.06-8.4, P = 0.038), LPFS (HR 3.08, 95% CI 1.27-7.50, P = 0.013), and DMFS (HR 1.84, 95% CI 0.99-3.42, P = 0.054). Taking OS as the main observation point, the best cut-off point of NTR was 0.95. Kaplan-Meier results showed that the 3-year OS (97.0% vs 85.4%, χ = 11.25, P = 0.001), 3-year LPFS (91.3% vs 82.1%, χ = 4.035, P = 0.045), and 3-year DMFS (92.3% vs 87.9%, χ = 4.576, P = 0.032) of patients with NTR < 0.95 were higher than those with NTR > 0.95.

Conclusions: High NTR before treatment indicates a poor prognosis for patients with nasopharyngeal carcinoma. This can serve as a reference value for the reasonable treatment and prognosis monitoring of such patients.
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http://dx.doi.org/10.1007/s00405-022-07562-wDOI Listing
August 2022

3D printed magnesium-doped β-TCP gyroid scaffold with osteogenesis, angiogenesis, immunomodulation properties and bone regeneration capability in vivo.

Biomater Adv 2022 May 17;136:212759. Epub 2022 Mar 17.

Department of Orthopedic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, China. Electronic address:

Bioceramics have been used in orthopedic surgery for several years. Magnesium (Mg) is an essential element in bone tissue and plays an important role in bone metabolism. Mg-doped bioceramics has attracted the attention of researchers recently. However, the optimal doping amount of Mg in β-TCP and the immunomodulatory property of Mg-doped β-TCP (Mg-TCP) have not been determined yet. In this study, β-TCP scaffolds doped with different contents of magnesium oxide (0 wt%, 1 wt%, 3 wt%, and 5 wt%) with gyroid structure were printed by digital light processing (DLP) method, and the physicochemical and biological functions were then investigated. Mg-doping improved the physicochemical properties of the β-TCP scaffolds. In vitro experiments confirmed that the doping of Mg in β-TCP scaffolds promoted the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and angiogenic differentiation of endothelial progenitor cells (EPCs), where the 5Mg-TCP has the optimal properties when using the "one cell type" method. It was also found that all Mg-TCP facilitated the polarization of RAW264.7 cells to the M2 phenotype, especially the 3Mg-TCP. However, 3Mg-TCP displayed the optimal osteogenic and angiogenic potential when using a "multiple cell type" method, which referred to culturing the BMSCs or EPCs in the macrophage-conditioned medium. Finally, the in vivo experiments were conducted and the results confirmed that the 3Mg-TCP scaffolds possessed the satisfying bone defect repair capability both after 6 and 12 weeks of implantation. This study suggests that 3Mg-TCP scaffolds provide the optimal biological performance and thus have the potential for clinical translation.
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http://dx.doi.org/10.1016/j.bioadv.2022.212759DOI Listing
May 2022

Immune repertoire analysis of normal Chinese donors at different ages.

Cell Prolif 2022 Aug 4:e13311. Epub 2022 Aug 4.

Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou, China.

Objectives: This study investigated the characteristics of the immune repertoire in normal Chinese individuals of different ages.

Materials And Methods: In this study, all seven receptor chains from both B and T cells in peripheral blood of 16 normal Chinese individuals from two age groups were analyzed using high-throughput sequencing and dimer-avoided multiplex PCR amplification. Normal in this study is defined as no chronic, infectious or autoimmune disease within 6 months prior to blood draw.

Results: We found that compared with the younger group, the clonal expression of T-cell receptor repertoire increased in the older group, while diversity decreased. In addition, we found that the T-cell receptor repertoire was more significantly affected by age than the B-cell receptor repertoire, including significant differences in the use of the unique TCR-alpha and TCR-beta V-J gene combinations, in the two groups of normal participants. We further analyzed the degree of complementarity determining region 3 sequence sharing between the two groups, and found shared TCR-alpha, TCR-gamma, immunoglobulin-kappa and immunoglobulin-lambda chain complementarity determining region 3 sequences in all subjects.

Conclusion: Taken together, our study gives us a better understanding of the immune repertoire of different normal Chinese people, and these results can be applied to the treatment of age-related diseases. Immune repertoire analysis also allows us to observe participant's wellness, aiding in early-stage diagnosis.
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http://dx.doi.org/10.1111/cpr.13311DOI Listing
August 2022

Transcriptome Analysis of Moso Bamboo () Reveals Candidate Genes Involved in Response to Dehydration and Cold Stresses.

Front Plant Sci 2022 19;13:960302. Epub 2022 Jul 19.

College of Landscape Architecture, Sichuan Agricultural University, Chengdu, China.

Bamboo (Bambusoideae) belongs to the grass family (Poaceae) and has been utilized as one of the most important nontimber forest resources in the world. Moso bamboo () is a large woody bamboo with high ecological and economic values. Global climate change brings potential challenges to the normal growth of moso bamboo, and hence its production. Despite the release of moso bamboo genome sequence, the knowledge on genome-wide responses to abiotic stress is still limited. In this study, we generated a transcriptome data set with respect to dehydration and cold responses of moso bamboo using RNA-seq technology. The differentially expressed genes (DEGs) under treatments of dehydration and cold stresses were identified. By combining comprehensive gene ontology (GO) analysis, time-series analysis, and co-expression analysis, candidate genes involved in dehydration and cold responses were identified, which encode abscisic acid (ABA)/water deficit stress (WDS)-induced protein, late embryogenesis abundant (LEA) protein, 9-cis-epoxycarotenoid dioxygenase (NCED), anti-oxidation enzymes, transcription factors, etc. Additionally, we used , a dehydration-induced gene encoding an "atypical" LEA protein, as an example to validate the function of the identified stress-related gene in tolerance to abiotic stresses, such as drought and salt. In this study, we provided a valuable genomic resource for future excavation of key genes involved in abiotic stress responses and genetic improvement of moso bamboo to meet the requirement for environmental resilience and sustainable production.
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http://dx.doi.org/10.3389/fpls.2022.960302DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343960PMC
July 2022

Transient nuclear deformation primes epigenetic state and promotes cell reprogramming.

Nat Mater 2022 Aug 4. Epub 2022 Aug 4.

Department of Bioengineering, University of California Los Angeles, Los Angeles, CA, USA.

Cell reprogramming has wide applications in tissue regeneration, disease modelling and personalized medicine. In addition to biochemical cues, mechanical forces also contribute to the modulation of the epigenetic state and a variety of cell functions through distinct mechanisms that are not fully understood. Here we show that millisecond deformation of the cell nucleus caused by confinement into microfluidic channels results in wrinkling and transient disassembly of the nuclear lamina, local detachment of lamina-associated domains in chromatin and a decrease of histone methylation (histone H3 lysine 9 trimethylation) and DNA methylation. These global changes in chromatin at the early stage of cell reprogramming boost the conversion of fibroblasts into neurons and can be partially reproduced by inhibition of histone H3 lysine 9 and DNA methylation. This mechanopriming approach also triggers macrophage reprogramming into neurons and fibroblast conversion into induced pluripotent stem cells, being thus a promising mechanically based epigenetic state modulation method for cell engineering.
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http://dx.doi.org/10.1038/s41563-022-01312-3DOI Listing
August 2022

Novel aerosol detection platform for SARS‑CoV‑2: Based on specific magnetic nanoparticles adsorption sampling and digital droplet PCR detection.

Chin Chem Lett 2022 Jul 23. Epub 2022 Jul 23.

State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau 999078, China.

The SARS‑CoV‑2 virus is released from an infectious source (such as a sick person) and adsorbed on aerosols, which can form pathogenic microorganism aerosols, which can affect human health through airborne transmission. Efficient sampling and accurate detection of microorganisms in aerosols are the premise and basis for studying their properties and evaluating their hazard. In this study, we built a set of sub-micron aerosol detection platform, and carried out a simulation experiment on the SARS‑CoV‑2 aerosol in the air by wet-wall cyclone combined with immunomagnetic nanoparticle adsorption sampling and ddPCR. The feasibility of the system in aerosol detection was verified, and the influencing factors in the detection process were experimentally tested. As a result, the sampling efficiency was 29.77%, and extraction efficiency was 98.57%. The minimum detection limit per unit volume of aerosols was 250 copies (10 copies/mL, concentration factor 2.5).
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http://dx.doi.org/10.1016/j.cclet.2022.07.044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308147PMC
July 2022

Unlock Layered Double Hydroxide as a High Performance Cathode Material for Aqueous Zinc Ion Batteries.

Adv Mater 2022 Jul 28:e2204320. Epub 2022 Jul 28.

State Key Laboratory of Chemical Resource Engineering, College of Chemistry, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, P. R. China.

Advanced cathode materials play an important role in promoting aqueous battery technology for safe energy storage. Transition metal double hydroxides are usually elusive as a stable cathode for aqueous zinc ion batteries (AZIBs) due to their unstable crystal structure, sluggish ion transportation and insufficient active sites for zinc ion storage. Here we report a trinary layered double hydroxide with hydrogen vacancies (Ni Mn Fe -LDH) as a new cathode material for AZIBs. A reversible high capacity up to 328 mAh g can be obtained and cycle stably over 500 cycles with a capacity retention of 85%. Experimental and theoretical studies reveal that the hydrogen vacancies in LDH could expose lattice oxygen atoms as active sites for zinc ion storage and accelerate ion diffusion by reducing the electrostatic interactions between zinc ions and the host structure. Besides, the synergy of trinary transitional metal cations can suppress the Jahn-Teller distortion of manganese (III) oxide octahedron and enable long cycle stability. This work provides not only a series of high performance cathode materials for aqueous zinc ion batteries but also a novel materials design strategy that can be extended to other multi-valence metal ion batteries. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202204320DOI Listing
July 2022

A WRKY Protein, MfWRKY40, of Resurrection Plant Plays a Positive Role in Regulating Tolerance to Drought and Salinity Stresses of .

Int J Mol Sci 2022 Jul 24;23(15). Epub 2022 Jul 24.

Department of Plant Sciences, University of California Davis, Davis, CA 95616, USA.

WRKY transcription factors (TFs), one of the largest transcription factor families in plants, play an important role in abiotic stress responses. The resurrection plant, , has a strong tolerance to dehydration, but only a few WRKY proteins related to abiotic stress response have been identified and functionally characterized in . In this study, we identified an early dehydration-induced gene, , of . The deduced MfWRKY40 protein has a conserved WRKY motif but lacks a typical zinc finger motif in the WRKY domain and is localized in the nucleus. To investigate its potential roles in abiotic stresses, we overexpressed in and found that transgenic lines exhibited better tolerance to both drought and salt stresses. Further detailed analysis indicated that MfWRKY40 promoted primary root length elongation and reduced water loss rate and stomata aperture (width/length) under stress, which may provide the better water uptake and retention abilities. MfWRKY40 also facilitated osmotic adjustment under drought and salt stresses by accumulating more osmolytes, such as proline, soluble sugar, and soluble protein. Additionally, the antioxidation ability of transgenic lines was also significantly enhanced, represented by higher chlorophyll content, less malondialdehyde and reactive oxygen species accumulations, as well as higher antioxidation enzyme activities. All these results indicated that MfWRKY40 might positively regulate tolerance to drought and salinity stresses. Further investigation on the relationship of the missing zinc finger motif of MfWRKY40 and its regulatory role is necessary to obtain a better understanding of the mechanism underlying the excellent drought tolerance of .
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http://dx.doi.org/10.3390/ijms23158145DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330732PMC
July 2022

Hippocampal semaphorin 3B improves depression-like behaviours in mice by upregulating synaptic plasticity and inhibiting neuronal apoptosis.

J Neurochem 2022 Jul 26. Epub 2022 Jul 26.

Hebei Key Laboratory of Neurophysiology, Shijiazhuang, China.

Depression is a global health problem, and there is a pressing need for a better understanding of its pathogenesis. Semaphorin 3B (Sema 3B) is an important axon guidance molecule that is primarily expressed in neurons and contributes to synaptic plasticity. Our previous studies using a high-throughput microarray assay suggested that Sema 3B expression was tremendously decreased during the development of depression, but the specific role and mechanisms of Sema 3B in depression are still unknown. Herein, we report that levels of Sema 3B protein are decreased in the hippocampus and serum of chronic mild stress (CMS)-treated mice. Increasing the levels of Sema 3B, either by injecting AAV-Sema 3B into the hippocampus or by injecting recombinant Sema 3B protein into the lateral ventricles, alleviated CMS-induced depression-like behaviours and enhanced the resistance to acute stress by increasing dendritic spine density in hippocampal neurons. In contrast, interfering with the function of Sema 3B by injecting anti-Sema 3B antibody into the lateral ventricles decreased the resistance to acute stress. In vitro, corticosterone (CORT) treatment decreased the survival rate and protein levels of Sema 3B and synapse-associated proteins in HT22 cells. Overexpression of Sema 3B improved the decreased survival rate caused by CORT by inhibiting apoptosis and increasing levels of synaptic-associated proteins, and knockdown of Sema 3B reduces the cellular resistance to CORT and the levels of synapse-associated proteins. These findings represent the first evidence for the neuroprotective mechanism of Sema 3B against stresses, suggesting that Sema 3B could be a promising novel target for the prevention and treatment of depression.
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http://dx.doi.org/10.1111/jnc.15680DOI Listing
July 2022

MSPJ: Discovering potential biomarkers in small gene expression datasets ensemble learning.

Comput Struct Biotechnol J 2022 14;20:3783-3795. Epub 2022 Jul 14.

Department of Neurosurgery, Xinqiao Hospital, The Army Medical University, Chongqing 400037, China.

In transcriptomics, differentially expressed genes (DEGs) provide fine-grained phenotypic resolution for comparisons between groups and insights into molecular mechanisms underlying the pathogenesis of complex diseases or phenotypes. The robust detection of DEGs from large datasets is well-established. However, owing to various limitations (e.g., the low availability of samples for some diseases or limited research funding), small sample size is frequently used in experiments. Therefore, methods to screen reliable and stable features are urgently needed for analyses with limited sample size. In this study, MSPJ, a new machine learning approach for identifying DEGs was proposed to mitigate the reduced power and improve the stability of DEG identification in small gene expression datasets. This ensemble learning-based method consists of three algorithms: an improved multiple random sampling with -analysis, SVM-RFE (support vector machines-recursive feature elimination), and permutation test. MSPJ was compared with ten classical methods by 94 simulated datasets and large-scale benchmarking with 165 real datasets. The results showed that, among these methods MSPJ had the best performance in most small gene expression datasets, especially those with sample size below 30. In summary, the MSPJ method enables effective feature selection for robust DEG identification in small transcriptome datasets and is expected to expand research on the molecular mechanisms underlying complex diseases or phenotypes.
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http://dx.doi.org/10.1016/j.csbj.2022.07.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304602PMC
July 2022

Metal-Organic Frameworks for Electrocatalytic Sensing of Hydrogen Peroxide.

Molecules 2022 Jul 18;27(14). Epub 2022 Jul 18.

Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Jiangsu Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Institute of Energy Supply Technology for High-End Equipment, School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing 210044, China.

The electrochemical detection of hydrogen peroxide (HO) has become more and more important in industrial production, daily life, biological process, green energy chemistry, and other fields (especially for the detection of low concentration of HO Metal organic frameworks (MOFs) are promising candidates to replace the established HO sensors based on precious metals or enzymes. This review summarizes recent advances in MOF-based HO electrochemical sensors, including conductive MOFs, MOFs with chemical modifications, MOFs-composites, and MOF derivatives. Finally, the challenges and prospects for the optimization and design of HO electrochemical sensors with ultra-low detection limit and long-life are presented.
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http://dx.doi.org/10.3390/molecules27144571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316108PMC
July 2022

Clinical Outcomes of Different Calcified Culprit Plaques in Patients with Acute Coronary Syndrome.

J Clin Med 2022 Jul 11;11(14). Epub 2022 Jul 11.

Department of Cardiology, The 2nd Affiliated Hospital of Harbin Medical University, Harbin 150086, China.

Background: Previous studies have found that coronary artery calcification is closely associated with the occurrence of major adverse cardiac events (MACE). This study aimed to investigate the characteristics and clinical outcomes of different calcified plaques in patients with acute coronary syndrome (ACS) by using optical coherence tomography (OCT).

Methods: 258 ACS patients with calcified culprit plaques who underwent OCT-guided stent implantation were enrolled. They were divided into three subtypes based on the calcified plaque morphology, including eruptive calcified nodules, calcified protrusion, and superficial calcific sheet.

Results: Compared with superficial calcific sheet and calcified protrusion, eruptive calcified nodules had the greatest calcium burden and a higher rate of stent edge dissection ( < 0.001) and incomplete stent apposition ( < 0.001). In a median follow-up period of 2 years, 39 (15.1%) patients experienced MACE (a composite event of cardiac death, target-vessel myocardial infarction, ischemia-driven revascularization), with a significantly higher incidence in the eruptive calcified nodules group (32.1% vs. 10.1% vs. 13.0%, = 0.001). A multivariate Cox analysis demonstrated that the eruptive calcified nodules (hazard ratio 3.14; 95% confidence interval, 1.64-6.02; = 0.001) were an independent predictor of MACE.

Conclusions: MACE occurred more frequently in ACS patients with eruptive calcified nodules, and the eruptive calcified nodules were an independent predictor of MACE.
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http://dx.doi.org/10.3390/jcm11144018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316434PMC
July 2022

Magnetic liquid metal scaffold with dynamically tunable stiffness for bone tissue engineering.

Biomater Adv 2022 Aug 8;139:212975. Epub 2022 Jun 8.

State Key Laboratory of New Textile Materials and Advanced Processing Technologies, Wuhan Textile University, Wuhan 430200, PR China. Electronic address:

The stiffness of most biomaterials used in bone tissue engineering is static at present, and does not provide an ideal biomimetic dynamical mechanical microenvironment for bone regeneration. To simulate the dynamic stiffness better during bone repair, the preparation of dynamic materials, especially hydrogels, has aroused researchers' interest. However, there are still many problems limiting the development of hydrogels such as small-scale stiffness changes and unstable mechanical properties. Here, magnetic liquid metal (MLM) was introduced into bone tissue engineering for the first time. A MLM scaffold was obtained by adding magnetic silicon dioxide particles ([email protected]) into galinstan. Furthermore, a porous MLM (PMLM) scaffold was obtained by adding polyethylene glycol as a template to the MLM scaffold. Both scaffolds can respond to external magnetic fields, so changing the magnetic field intensity can achieve a large-scale of dynamic stiffness change. The results showed that the MLM scaffold has good biocompatibility and can promote the osteogenic differentiation of mesenchymal stem cells (MSCs). The PMLM scaffold with dynamic stiffness can promote new bone regeneration and osseointegration in vivo. Our research will open up a new field for the application of liquid metal and bring new ideas for the development of bone tissue engineering materials.
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http://dx.doi.org/10.1016/j.bioadv.2022.212975DOI Listing
August 2022

binding affinity prediction for metabotropic glutamate receptors using both endpoint free energy methods and a machine learning-based scoring function.

Phys Chem Chem Phys 2022 Aug 3;24(30):18291-18305. Epub 2022 Aug 3.

Department of Pharmaceutical Sciences and Computational Chemical Genomics Screening Center, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA.

Metabotropic glutamate receptors (mGluRs) play an important role in regulating glutamate signal pathways, which are involved in neuropathy and periphery homeostasis. mGluR4, which belongs to Group III mGluRs, is most widely distributed in the periphery among all the mGluRs. It has been proved that the regulation of this receptor is involved in diabetes, colorectal carcinoma and many other diseases. However, the application of structure-based drug design to identify small molecules to regulate the mGluR4 receptor is limited due to the absence of a resolved mGluR4 protein structure. In this work, we first built a homology model of mGluR4 based on a crystal structure of mGluR8, and then conducted hierarchical virtual screening (HVS) to identify possible active ligands for mGluR4. The HVS protocol consists of three hierarchical filters including Glide docking, molecular dynamic (MD) simulation and binding free energy calculation. We successfully prioritized active ligands of mGluR4 from a set of screening compounds using HVS. The predicted active ligands based on binding affinities can almost cover all the experiment-determined active ligands, with only one ligand missed. The correlation between the measured and predicted binding affinities is significantly improved for the MM-PB/GBSA-WSAS methods compared to the Glide docking method. More importantly, we have identified hotspots for ligand binding, and we found that SER157 and GLY158 tend to contribute to the selectivity of mGluR4 ligands, while ALA154 and ALA155 could account for the ligand selectivity to mGluR8. We also recognized other 5 key residues that are critical for ligand potency. The difference of the binding profiles between mGluR4 and mGluR8 can guide us to develop more potent and selective modulators. Moreover, we evaluated the performance of IPSF, a novel type of scoring function trained by a machine learning algorithm on residue-ligand interaction profiles, in guiding drug lead optimization. The cross-validation root-mean-square errors (RMSEs) are much smaller than those by the endpoint methods, and the correlation coefficients are comparable to the best endpoint methods for both mGluRs. Thus, machine learning-based IPSF can be applied to guide lead optimization, albeit the total number of actives/inactives are not big, a typical scenario in drug discovery projects.
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http://dx.doi.org/10.1039/d2cp01727jDOI Listing
August 2022

Development and Validation of a Prediction Rule for Major Adverse Cardiac and Cerebrovascular Events in High-Risk Myocardial Infarction Patients After Primary Percutaneous Coronary Intervention.

Clin Interv Aging 2022 18;17:1099-1111. Epub 2022 Jul 18.

Fuwai Hospital, Chinese Academy of Medical Sciences, Shenzhen, People's Republic of China.

Background And Aims: We aimed to develop a clinical prediction tool to improve the prognosis of major adverse cardiac and cerebrovascular events (MACCE) among high-risk myocardial infarction (MI) patients undergoing primary percutaneous coronary intervention (PCI).

Methods: The present study was a prospective and observational study. A total of 4151 consecutive MI patients who underwent primary PCI at Fuwai Hospital in Beijing, China (January 2010 and June 2017) were enrolled. Forty-eight patients without follow-up data were excluded from the study. The pre-specified criteria (Supplementary Information 1) were chosen to enroll MI patients at high risk for MACCE complications after PCI.

Results: The full model included seven variables, with a risk score of 160 points. Derivation and validation cohort models predicting MACCE had C-statistics of 0.695 and 0.673. The area under the curve (AUC) of the survival receiver operating characteristic curve (ROC) for predicting MACCE was 0.991 and 0.883 in the derivation and validation cohorts, respectively.

Conclusion: The predicted model was internally validated and calibrated in large cohorts of patients with high-risk MI receiving primary PCI to predict MACCE and showed modest accuracy in the derivation and validation cohorts.
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http://dx.doi.org/10.2147/CIA.S358761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307870PMC
July 2022

Editorial: Viral infection and brain diseases.

Authors:
Song Li Weidong Le

Brain Res Bull 2022 Jul 21;188:108-109. Epub 2022 Jul 21.

Liaoning Provincial Key Laboratory for Research on the Pathogenic Mechanisms of Neurological Diseases, The First Affiliated Hospital, Dalian Medical University, Dalian, China; Institute of Neurology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China; Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, China. Electronic address:

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http://dx.doi.org/10.1016/j.brainresbull.2022.07.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299986PMC
July 2022

Microplastics distribution in different habitats of Ximen Island and the trapping effect of blue carbon habitats on microplastics.

Mar Pollut Bull 2022 Aug 20;181:113912. Epub 2022 Jul 20.

Ocean College, Zhejiang University, Zhoushan 316021, China. Electronic address:

Sediments are considered to be important sinks of microplastics, but the enrichment process of microplastics by blue carbon ecosystems is poorly studied. This study analyzed the spatial distribution and temporal changes, assessed the polymer types and morphological characteristics of microplastics in sediments of five ecosystems, i.e. forests, paddy fields, mangroves, saltmarshes and bare beaches on Ximen Island, Yueqing Bay, China. The trapping effect of blue carbon (mangrove and saltmarsh) sediments on microplastic was further explored. Temporal trends in microplastic abundance showed a significant increase over the last 20 years, with the enrichment of microplastics in mangrove and saltmarsh sediments being 1.7 times as high as that in bare beach, exhibiting blue carbon vegetations have strong enrichment effect on microplastics. The dominant color, shape, size, and polymer type of microplastics in sediments were transparent, fibers and fragments, <1 mm, and polyethylene, respectively. Significant differences in the abundance and characteristics of microplastics between intertidal sediments and terrestrial soils reveal that runoff input is the main source of microplastics. This study provided the evidence of blue carbon habitats as traps of microplastics.
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http://dx.doi.org/10.1016/j.marpolbul.2022.113912DOI Listing
August 2022

Comparative metabolomics and transcriptomics analyses provide insights into the high-yield mechanism of phenazines biosynthesis in Pseudomonas chlororaphis GP72.

J Appl Microbiol 2022 Jul 23. Epub 2022 Jul 23.

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Aims: Phenazines, such as phenazine-1-carboxylic acid (PCA), phenazine-1-carboxamide (PCN), 2-hydroxyphenazine-1-carboxylic acid (2-OH-PCA), 2-hydroxyphenazine (2-OH-PHZ), are a class of secondary metabolites secreted by plant-beneficial Pseudomonas. Ps. chlororaphis GP72 utilizes glycerol to synthesize PCA, 2-OH-PCA and 2-OH-PHZ, exhibiting broad-spectrum antifungal activity. Previous studies showed that the addition of dithiothreitol (DTT) could increase the phenazines production in Ps. chlororaphis GP72AN. However, the mechanism of high yield of phenazine by adding DTT is still unclear.

Methods And Results: In this study, untargeted and targeted metabolomic analysis were adopted to determine the content of metabolites. The results showed that the addition of DTT to GP72AN affected the content of metabolites of central carbon metabolism, shikimate pathway and phenazine competitive pathway. Transcriptome analysis was conducted to investigate the changed cellular process, and the result indicated that the addition of DTT affected the expression of genes involved in phenazine biosynthetic cluster and genes involved in phenazine competitive pathway, driving more carbon flux into phenazine biosynthetic pathway. Furthermore, genes involved in antioxidative stress, phosphate transport system and mexGHI-opmD efflux pump were also affected by adding DTT.

Conclusion: This study demonstrated that the addition of DTT altered the expression of genes related to phenazine biosynthesis, resulting in the change of metabolites involved in central carbon metabolism, shikimate pathway and phenazine competitive pathway.

Significance And Impact Of The Study: This work expands the understanding of high yield of phenazine by the addition of DTT and provides several targets for increasing phenazine production.
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http://dx.doi.org/10.1111/jam.15727DOI Listing
July 2022

Engineering stem cell therapeutics for cardiac repair.

J Mol Cell Cardiol 2022 Jul 18;171:56-68. Epub 2022 Jul 18.

Department of Bioengineering, Department of Medicine, University of California, Los Angeles, Los Angeles, California 90095, USA; Eli and Edythe Broad Stem Cell Research Center, University of California, Los Angeles, California 90095, USA. Electronic address:

Cardiovascular disease is the leading cause of death in the world. Stem cell-based therapies have been widely investigated for cardiac regeneration in patients with heart failure or myocardial infarction (MI) and surged ahead on multiple fronts over the past two decades. To enhance cellular therapy for cardiac regeneration, numerous engineering techniques have been explored to engineer cells, develop novel scaffolds, make constructs, and deliver cells or their derivatives. This review summarizes the state-of-art stem cell-based therapeutics for cardiac regeneration and discusses the emerged bioengineering approaches toward the enhancement of therapeutic efficacy of stem cell therapies in cardiac repair. We cover the topics in stem cell source and engineering, followed by stem cell-based therapies such as cell aggregates and cell sheets, and biomaterial-mediated stem cell therapies such as stem cell delivery with injectable hydrogel, three-dimensional scaffolds, and microneedle patches. Finally, we discuss future directions and challenges of engineering stem cell therapies for clinical translation.
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http://dx.doi.org/10.1016/j.yjmcc.2022.06.013DOI Listing
July 2022

Polarized Cu-Bi Site Pairs for Non-Covalent to Covalent Interaction Tuning toward N Photoreduction.

Adv Mater 2022 Jul 21:e2204959. Epub 2022 Jul 21.

School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.

A universal atomic layer confined doping strategy is developed to prepare Bi O Br materials incorporating isolated Cu atoms. The local polarization can be created along the CuOBi atomic interface, which enables better electron delocalization for effective N activation. The optimized Cu-Bi O Br atomic layers show 5.3× and 88.2× improved photocatalytic nitrogen fixation activity than Bi O Br atomic layer and bulk Bi O Br , respectively, with the NH generation rate reaching 291.1 µmol g h in pure water. The polarized Cu-Bi site pairs can increase the non-covalent interaction between the catalyst's surface and N molecules, then further weaken the covalent bond order in NN. As a result, the hydrogenation pathways can be altered from the associative distal pathway for Bi O Br to the alternating pathway for Cu-Bi O Br . This strategy provides an accessible pathway for designing polarized metal site pairs or tuning the non-covalent interaction and covalent bond order.
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http://dx.doi.org/10.1002/adma.202204959DOI Listing
July 2022

Fast constructing polarity-switchable zinc-bromine microbatteries with high areal energy density.

Sci Adv 2022 Jul 13;8(28):eabo6688. Epub 2022 Jul 13.

Key Laboratory of Organic Optoelectronics and Molecular Engineering of Ministry of Education, Department of Chemistry, Tsinghua University, 100084 Beijing, P. R. China.

Microbatteries (MBs) are promising candidates to provide power for various miniaturized electronic devices, yet they generally suffer from complicated fabrication procedures and low areal energy density. Besides, all cathodes of current MBs are solid state, and the trade-off between areal capacity and reaction kinetics restricts their wide applications. Here, we propose a dual-plating strategy to facilely prepare zinc-bromine MBs (Zn-Br MBs) with a liquid cathode to achieve both high areal energy density and fast kinetics simultaneously. The Zn-Br MBs deliver a record high areal energy density of 3.6 mWh cm, almost an order of magnitude higher than available planar MBs. Meanwhile, they show a polarity-switchable feature to tolerate confusion of cathode and anode. This strategy could also be extended to other battery systems, such as Zn-I and Zn-MnO MBs. This work not only proposes an effective construction method for MBs but also enriches categories of microscale energy storage devices.
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http://dx.doi.org/10.1126/sciadv.abo6688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9278868PMC
July 2022

Sodium butyrate inhibits osteogenesis in human periodontal ligament stem cells by suppressing smad1 expression.

BMC Oral Health 2022 07 19;22(1):301. Epub 2022 Jul 19.

Department of Orthodontics, School of Stomatology, Capital Medical University, Tian Tan Xi Li No.4, Beijing, 100050, People's Republic of China.

Background: Butyrate is a major subgingival microbial metabolite that is closely related to periodontal disease. It affects the proliferation and differentiation of mesenchymal stem cells. However, the mechanisms by which butyrate affects the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) remain unclear. Here, we investigated the effect of sodium butyrate (NaB) on the osteogenic differentiation of human PDLSCs.

Methods: PDLSCs were isolated from human periodontal ligaments and treated with various concentrations of NaB in vitro. The cell counting kit-8 assay and flow cytometric analysis were used to assess cell viability. The osteogenic differentiation capabilities of PDLSCs were evaluated using the alkaline phosphatase activity assay, alizarin red staining, RT-PCR, western blotting and in vivo transplantation.

Results: NaB decreased PDLSC proliferation and induced apoptosis in a dose- and time-depend manner. Additionally, 1 mM NaB reduced alkaline phosphatase activity, mineralization ability, and the expression of osteogenic differentiation-related genes and proteins. Treatment with a free fatty acids receptor 2 (FFAR2) antagonist and agonist indicated that NaB inhibited the osteogenic differentiation capacity of PDLSCs by affecting the expression of Smad1.

Conclusion: Our findings suggest that NaB inhibits the osteogenic differentiation of PDLSCs by activating FFAR2 and decreasing the expression of Smad1.
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http://dx.doi.org/10.1186/s12903-022-02255-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297574PMC
July 2022

Unusual mature teratomas in the right ventricle.

Eur Heart J Cardiovasc Imaging 2022 Jul 19. Epub 2022 Jul 19.

Department of Ultrasound, Beijing Anzhen Hospital, Capital Medical University; Beijing Key Laboratory of Maternal-Fetus Medicine in Fetal Heart Disease (BZ0308), No. 2, Anzhenli, Chaoyang District, Beijing 100029, People's Republic of China.

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http://dx.doi.org/10.1093/ehjci/jeac144DOI Listing
July 2022

Vimentin binds to a novel tumor suppressor protein, GSPT1-238aa, encoded by circGSPT1 with a selective encoding priority to halt autophagy in gastric carcinoma.

Cancer Lett 2022 Jul 13;545:215826. Epub 2022 Jul 13.

Guangdong Provincial Key Laboratory of Genome Stability and Disease Prevention and Regional Immunity and Diseases, Department of Pathology, Shenzhen University School of Medicine, Shenzhen, Guangdong, 518060, PR China. Electronic address:

Circular RNAs (circRNAs) are covalently closed, endogenous molecules that are widespread in eukaryotes. Recent evidence indicates that circRNAs play important roles in carcinogenesis. Several circRNAs have been reported to comprise translatable RNA; however, whether circRNAs encode functional proteins remains unknown. In our study, circRNA sequencing was carried out using five pathologically diagnosed gastric carcinoma (GC) samples and their paired adjacent normal tissues, we characterized the circRNA GSPT1 (circGSPT1), which is expressed at low levels in GC. Antibody detections, and mass spectrometry were used to validate active circRNA translation. The spanning junction open reading frame in circGSPT1, driven by an internal ribosome entry site (IRES), encodes a functional peptide, termed GSPT1-238aa. Interestingly, GSPT1-238aa tends to select the start codon used to initiate translation. This is the first finding of selective translation driven by IRES. CircGSPT1 and GSPT1-238aa halted the proliferation, migration, and invasion in GC cells in vitro. We also confirmed that the vimentin/Beclin1/14-3-3 complex interacts with GSPT1-238aa and modulates autophagy via the PI3K/AKT/mTOR signaling pathway in GC cells. Our study reveals that GSPT1-238aa, a novel protein encoded by circGSPT1, halts GC tumorigenesis. We also provide insights into the function and underlying molecular mechanisms of GSPT1-238aa in GC and suggest that this protein represents a novel target for GC treatment.
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http://dx.doi.org/10.1016/j.canlet.2022.215826DOI Listing
July 2022
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