Publications by authors named "Li Niu"

308 Publications

Identifying diurnal cortisol profiles among young adults: Physiological signatures of mental health trajectories.

Psychoneuroendocrinology 2021 Mar 17;128:105204. Epub 2021 Mar 17.

Department of Biostatistics and Department of Social and Behavioral Sciences, School of Global Public Health, New York University, New York, NY, USA.

Previous research has used cortisol, the major hormonal byproduct of the hypothalamic pituitary adrenal (HPA) axis system, to explore how environmental stressors influence daily physiological functioning. Most of the research focused on diurnal cortisol has examined specific cortisol markers, with little consideration of how different components of the diurnal pattern may co-occur. Morning level, cortisol awakening response (CAR), bedtime level, as well as the diurnal slope and total cortisol exposure throughout the day (area under the curve; AUC), are five common parameters of diurnal HPA axis functioning that have been individually linked to physical and mental health outcomes, with mixed results. The current study introduces a novel approach to capture heterogeneity in HPA axis activity by using latent profile analysis to generate empirically-derived, theoretically supported diurnal cortisol profiles based on all five indicators. We analyzed salivary cortisol data from 278 young adults during a time of heightened sociopolitical stress - the 2016 U.S. presidential election - and examined whether profiles differentially predicted mental health trajectories across six months. Findings suggest that a specific combination of cortisol parameters (i.e., flat slope, high AUC, and high CAR) may predict worse mental health risk over time. Overall, this work suggests that diurnal cortisol profiles likely reflect distinct physiological underpinnings with unique health consequences that may not be observed by studying individual cortisol parameters.
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http://dx.doi.org/10.1016/j.psyneuen.2021.105204DOI Listing
March 2021

Targeted exome sequencing identifies mutational landscape in a cohort of 1500 Chinese patients with non-small cell lung carcinoma (NSCLC).

Hum Genomics 2021 Apr 12;15(1):21. Epub 2021 Apr 12.

Department of Radiation Oncology, The First Affiliated Hospital of Anhui Medical University, No.218, Jixi Road, Hefei, 230022, Anhui, China.

Background: Non-small cell lung carcinoma (NSCLC) is one of the most common human cancers, comprising approximately 80-85% of all lung carcinomas. An estimated incidence of NSCLC is approximately 2 million new cases per year worldwide.

Results: In recent decade, the treatment of NSCLC has made breakthrough progress owing to a large number of targeted therapies which were approved for clinical use. Epidemiology, genetic susceptibility, and molecular profiles in patients are likely to play an important factor in response rates and survival benefits to these targeted treatments and thus warrant further investigation on ethnic differences in NSCLC. In this study, a total number of 1500 Chinese patient samples,1000 formalin fixed paraffin-embedded (FFPE) and 500 blood samples, from patients with NSCLC were analyzed by targeted sequencing to explore mutational landscape in ethnic groups associated with China.

Conclusions: Overall, the data presented here provide a comprehensive analysis of NSCLC mutational landscape in Chinese patients and findings are discussed in the context of similar studies on different ethnic groups.
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http://dx.doi.org/10.1186/s40246-021-00320-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042687PMC
April 2021

Daily academic satisfaction and ethnic/racial identity of Asian American adolescents: The role of objective and subjective peer diversity at school.

Asian Am J Psychol 2020 Jun 21;11(2):59-68. Epub 2019 Nov 21.

Department of Psychology Fordham University, 441 East Fordham Rd. Bronx, NY 10458.

The purpose of the current study was to examine the association between daily academic satisfaction and ethnic/racial identity of Asian American adolescents. Based on ecological systems theory and social comparison theory the moderating roles of objective and subjective peer diversity at school in this association were also examined. Daily diary and survey responses of 102 Asian American adolescents were included in this study (Age: =15.26, =0.71; Female=70.60%; US-born=74.50%). Using hierarchical linear modeling, no direct association between daily academic satisfaction and ethnic/racial identity was observed in the full sample. However, when objective and subjective peer diversity at school were considered a positive association was observed among the adolescents who perceived a high percentage of Asian American peers at school. The interpretation and implications for the results are discussed.
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http://dx.doi.org/10.1037/aap0000168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7989802PMC
June 2020

Effects of methyl cellulose and soybean protein isolate coating on oil content and chemical hazards of Chinese fried dough cakes.

J Food Prot 2021 Mar 26. Epub 2021 Mar 26.

Guangzhou University.

The increase in fat-related diseases and chemical hazards produced during the frying process poses a major threat to human health. Coating has been used as a practical method to reduce the oil content and chemical hazard of fried foods. Methyl cellulose (MC) and soy protein isolate (SPI) were used as coating materials to pretreat Chinese fried dough cake (CFDC) before frying. The results showed that 1.5% MC was the best choice for coating to simultaneously balance a low oil content and low chemical hazard content of CFDC. The CFDC prepared using the 1.5% MC content had the following components: an oil content of 11.3%, an acrylamide value of 73.70 μg/kg, an acid value of 0.15 mg KOH/100 kg, a peroxide value of 8.54 mmol/kg, a p-anisidine value of 6.36, a malondialdehyde content of 0.36 μg/g, a 4-hydroxy-2-(E)-hexenal content of 0.13 μg/g, a 4-hydroxy-2-(E)-nonenal content of 0.51 μg/g, and a glycidyl ester content of 4272 μg/kg. MC and SPI enhanced the oil barrier of the coating film, which effectively reduced the heat transfer coefficients, oil content, oil oxidation and chemical hazard in the CFDC. Our work contributes to the final control of oil content and chemical hazard in fried food by applying an edible coating.
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http://dx.doi.org/10.4315/JFP-21-062DOI Listing
March 2021

mA-Mediated Upregulation of LINC00857 Promotes Pancreatic Cancer Tumorigenesis by Regulating the miR-150-5p/E2F3 Axis.

Front Oncol 2021 18;11:629947. Epub 2021 Feb 18.

Department of Hepatopancreatobiliary Surgery, The Third Xiangya Hospital, Central South University, Changsha, China.

The mortality and morbidity rates of pancreatic cancer (PC) have been increasing over the past two decades. Recent evidence indicates that long non-coding RNAs (lncRNAs) are usually dysregulated in the tumorigenesis and progression of PC. In the present study, we showed that the expression of LINC00857 was upregulated in PC and associated with poor prognosis based on the Gene Expression Profiling Interactive Analysis (GEPIA) database and validated in our PC tissues and cell lines. N-Methyladenosine (mA) was highly enriched within LINC00857 and enhanced its RNA stability. Knockdown of LINC00857 remarkably inhibited the proliferation and promoted the apoptosis of PC cells. Then, by using bioinformation analysis and verified experiments, we identified that LINC00857 functioned as a competing endogenous RNA (ceRNA) for sponging miR-150-5p, leading to the upregulation of its target E2F3 in PC cells. Taken above, our study revealed a potential ceRNA regulatory pathway in which LINC00857 modulates E2F3 expression by binding to miR-150-5p, ultimately promoting tumorigenesis in PC. LINC00857/miR-150-5p/E2F3 regulatory axis may be taken as an alternative therapeutic target for treating PC.
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http://dx.doi.org/10.3389/fonc.2021.629947DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930559PMC
February 2021

Polymer Electrochemiluminescence Featuring Thermally Activated Delayed Fluorescence.

Chemphyschem 2021 Feb 23. Epub 2021 Feb 23.

Centre for Advanced Analytical Science, c/o School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, 510006, P. R. China.

Electrochemiluminescence (ECL) based on conjugated polymers or oligomers is persistently being pursued owing to its huge application scope ranging from ultra-sensitive bioanalysis to ultra-resolution imaging and spectroscopy. Because of the theoretical limit in radiative exciton generation yield (typically ∼25 %) of those polymers or oligomers, the corresponding ECL efficiency is still limited, which hampers its ECL performance and its related applications. Herein, we report ECL based on a thermally activated delayed fluorescence (TADF) polymer scaffold, which is characteristic of all-exciton harvesting in the ECL process, and thus potentially capable of achieving ∼100 % ECL efficiency. These desired properties of the TADF polymer ECL is attributed to a fast and efficient up-conversion process from non-radiative triplet to radiative singlet states under thermal activation, which is absent in conventional fluorescent polymers/oligomers, such as F8BT. In this study, various ECL modes, including annihilation or co-reactant mode using TPrA or S O as co-reactant, are confirmed for our model TADF polymer ECL system, which was different from fluorescent polymer ECL counterpart. Furthermore, solid-state ECL sensing on L-cysteine (an important marker of disease) is also evaluated by using the model TADF polymer. Ultralow detection limit in combination with high sensitivity and good specificity are achieved for this model system, indicative of a high potential of the TADF polymer scaffold for applications in the broad field of ECL.
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http://dx.doi.org/10.1002/cphc.202100076DOI Listing
February 2021

Transcription-associated metabolomic adjustments in maize occur during combined drought and cold stress.

Plant Physiol 2021 Feb 4. Epub 2021 Feb 4.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Although simultaneous drought and cold stress occurs, especially in northwestern and eastern regions of China, and is an important factor limiting agricultural productivity, there are few studies focusing on plant responses to a combination of drought and cold stress. Here, by partially overlapping drought and cold stresses, we characterized the acclimation of maize (Zea mays B73) to these two stresses using physiological measurements, as well as comparative transcriptomics combined with metabolomics and hormonal analyses during the stress treatments and recovery stages. The combined drought and cold stress and drought stress alone were accompanied by a decline in photosynthetic capacity and enhanced transcriptional response, and subsequent recovery of these following removal from stress, whereas cold stress alone was accompanied by irreversible damage to photosynthetic capacity and chloroplast structure. The stress combination induced transcription-associated metabolomic alterations, in which raffinose, trehalose-6-phosphate, and proline accumulated, and monosaccharide abundance increased. Concomitantly, the increased abscisic acid (ABA) content and upregulated ABA signaling pathway may have provided the transcriptional regulation for the metabolic changes. In a parallel experiment, ABA treatments prior to exposure of the plants to cold stress primed the plants to survive the cold stress, thus confirming a key role for the endogenous ABA activated by the drought pretreatment in acclimation of the plants to cold. We present a model showing that the plant response to the combined stress is multi-faceted and reveal an ABA-dependent maize acclimation mechanism to the stress combination.
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http://dx.doi.org/10.1093/plphys/kiab050DOI Listing
February 2021

[Analysis of ALMS1 gene variants in seven patients with Alström syndrome].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2021 Feb;38(2):112-116

Department of Endocrinology and Metabolism, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.

Objective: To explore the genetic basis for 7 patients with Alström syndrome.

Methods: DNA was extracted from peripheral blood samples of the patients and their parents. Whole exome sequencing was carried out for the patients. Suspected variant was verified by Sanger sequencing and bioinformatic analysis.

Results: Genetic testing revealed 12 variants of the ALMS1 gene among the 7 patients, including 7 nonsense and 5 frameshift variants, which included c.5418delC (p.Tyr1807Thrfs*23), c.10549C>T (p.Gln3517*), c.9145dupC (p.Thr3049Asnfs*12), c.10819C>T (p.Arg3607*), c.5701_5704delGAGA (p.Glu1901Argfs*18), c.9154_9155delCT (p.Cys3053Serfs*9), c.9460delG (p.Val3154*), c.9379C>T (p.Gln3127*), c.12115C>T (p.Gln4039*), c.1468dupA (p.Thr490Asnfs*15), c.10825C>T (p.Arg3609*) and c.3902C>A (p.Ser1301*). Among these, c.9154_ 9155delCT, c.9460delG, c.9379C>T, and c.1468dupA were unreported previously. Based on the standards and guidelines of American College of Medical Genetics and Genomics, the c.9379C>T and c.12115C>T variants of the ALMS1 gene were predicted to be likely pathogenic (PVS1+PM2), whilst the other 10 variants were predicted to be pathogenic (PVS1+ PM2+ PP3+PP4).

Conclusion: ALMS1 variants probably underlay the Alström syndrome in the 7 patients, and genetic testing can provide a basis for the clinical diagnosis of this syndrome. The discovery of four novel variants has expanded the mutational spectrum of Alström syndrome.
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http://dx.doi.org/10.3760/cma.j.cn511374-20200115-00033DOI Listing
February 2021

Palladium-modified cuprous(i) oxide with {100} facets for photocatalytic CO reduction.

Nanoscale 2021 Feb 3;13(5):2883-2890. Epub 2021 Feb 3.

School of Civil Engineering c/o Center for Advanced Analytical Science, School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, P. R. China.

Using metal as a photohole capturer can promote the photoelectron of p-type copper(i) oxide (CuO) substrate for efficient carbon dioxide reduction. However, palladium-decorated CuO (CuO-Pd) is seldom reported due to their mismatching band arrangement. Herein, we have successfully established a matched band alignment between Pd nanoparticles and CuO with exposed {100} facets (100CuO). The high work function of 100CuO originating from T symmetry vibration facilitates the photohole transferring to Pd nanoparticles, which leads to a three-fold increase in the photocatalytic generation of carbon monoxide (100CuO-0.1Pd, 0.13 μmol g h) than that with pristine 100CuO (0.04 μmol g h). Besides, the incorporation of Pd can relieve the photocorrosion of 100CuO, thus promoting its photocatalytic stability. As a contrast, 111CuO (CuO exposed to {111} facets) with low-work function was also synthesized and no charge migration was observed between 111CuO and Pd species, which verified the important role of the crystal surface regulation. All experimental phenomena were certified by the crystal surface analysis and energy band structure construction. Moreover, CO adsorption capacity tests indicated that the incorporation of Pd is beneficial for the capture of CO molecules. We hope that this work to some extent will enrich the subject of photocatalytic CO reduction.
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http://dx.doi.org/10.1039/d0nr07703hDOI Listing
February 2021

circRNA on animal skeletal muscle development regulation.

Yi Chuan 2020 Dec;42(12):1178-1191

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

Circular RNA (circRNA) is a type of closed circular RNA molecules formed by reverse splicing, which exists widely in organisms and has become a research hotspot in non-coding RNAs in recent years. Skeletal muscle plays the role of coordinating movement and maintaining normal metabolism and endocrine in organisms. With the development of sequencing and bioinformatics analysis technology, the functions and regulation mechanisms of circRNAs in skeletal muscle development have been gradually revealed. In this review, we summarize the types of molecular regulatory mechanisms, the classical research ideas and the functional research methods of circRNAs, and the research progress of circRNAs involved in normal development of skeletal muscle and regulation of skeletal muscle disease, in order to provide a reference to further study of the genetic mechanisms of circRNAs in the regulation of skeletal muscle development.
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http://dx.doi.org/10.16288/j.yczz.20-207DOI Listing
December 2020

Electrochemically induced grafting of ferrocenyl polymers for ultrasensitive cleavage-based interrogation of matrix metalloproteinase activity.

Biosens Bioelectron 2021 Apr 20;178:113010. Epub 2021 Jan 20.

Center for Advanced Analytical Science, School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, 510006, PR China. Electronic address:

Being closely associated with a variety of physiological and pathological processes, matrix metalloproteinases (MMPs) are useful as potential targets for drug therapy and informative markers for disease diagnosis. On the basis of the electrochemically induced grafting of ferrocenyl polymers and the proteolytic cleavage of recognition peptide, a novel electrochemical sensor is presented in this work for the highly specific interrogation of MMP activities at ultralow levels. The recognition peptide, to be immobilized via the N-terminus, is free of carboxyl group. The presence of the target MMP would cleave the end-tethered recognition peptide, generating a free carboxyl group at the C-terminus of the rest fragment. To be used as the reversible addition-fragmentation chain-transfer (RAFT) agent, the dithiobenzoate, 4-cyano-4-(phenylcarbonothioylthio)pentanoic acid (CPAD), can therefore be tethered via the carboxylate-Zr(IV)-carboxylate chemistry. Subsequently, the grafting of ferrocenyl polymers through electrochemically induced RAFT (eRAFT) polymerization of ferrocenylmethyl methacrylate (FcMMA) would recruit a large quantity of Fc redox reporters on electrode surface. With benefits from the excellent specificity of the enzyme-substrate recognition, the presented cleavage-based sensor is highly selective. Under optimal conditions, the detection limit in the presence of MMP-2 as the model target can be as low as 0.27 pg mL, with a linear range from 1 pg mL to 1 ng mL. Furthermore, its applicability in the interrogation of MMP activity in complex serum samples and the screening of MMP inhibitors is satisfactory. The presented cleavage-based electrochemical MMP sensor is easy to fabricate and low-cost, thus showing great promise in drug discovery and disease diagnosis.
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http://dx.doi.org/10.1016/j.bios.2021.113010DOI Listing
April 2021

Coupling Plasmonic System for Efficient Wavefront Control.

ACS Appl Mater Interfaces 2021 Feb 21;13(4):5844-5852. Epub 2021 Jan 21.

School of Electrical and Computer Engineering, Oklahoma State University, Stillwater, Oklahoma 74078, United States.

Efficient and flexible manipulation of electromagnetic waves using metasurfaces has attracted continuous attention in recent years. However, previous studies mainly apply sole resonance effect to accomplish the task. Here, we show that introducing a meta-coupling effect would reveal further physical insights in the electromagnetic wave control. To demonstrate this, a reflection-type coupling system composed by two identical linear resonances in a metal-insulator-metal configuration is theoretically proposed using the coupled-mode theory, whose phase diagram can be well controlled upon the coupling changes. Such intriguing optical property is verified by a double C-shaped resonator in the terahertz regime, where the coupling effect can be tuned by changing their either relative distance or rotation. More importantly, the reflection phase shift around the working frequency can be efficiently engineered without having to change the dimensions of the resonators. Two efficient anomalous metasurface deflectors are designed and experimentally characterized, whose maximum measured efficiency is more than 70%. The proposed controlling strategy further enriches the designing freedoms of metasurfaces and may find broad applications in realizing efficient and tunable functional devices.
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http://dx.doi.org/10.1021/acsami.0c21120DOI Listing
February 2021

Oxidized titanium carbide MXene-enabled photoelectrochemical sensor for quantifying synergistic interaction of ascorbic acid based antioxidants system.

Biosens Bioelectron 2021 Apr 7;177:112978. Epub 2021 Jan 7.

State Key Laboratory of Electroanalytical Chemistry, C/o Engineering Laboratory for Modern Analytical Techniques, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China; University of Science and Technology of China, Hefei, 230026, China.

Antioxidants can protect organization from damage by scavenging of free radicals. When two kinds of antioxidants are consumed together, the total antioxidant capacity might be enhanced via synergistic interactions. Herein, we develop a simple, direct, and effective strategy to quantify the synergistic interaction between ascorbic acid (AA) and other different antioxidants by photoelectrochemical (PEC) technology. MXene TiC-TiO composites fabricated via hydrogen peroxide oxidation were applied as sensing material for the antioxidants interaction study. Under excitation of 470 nm wavelength, the photogenerated electrons transfer from the conduction band of TiO nanoparticles to the TiC layers, and the holes in TiO can oxidize antioxidants, leading to an enhanced photocurrent as the detection signal. This PEC sensor exhibits a good linear range to AA concentrations from 12.48 to 521.33 μM as well as obvious antioxidants capability synergism. In particular, the photocurrents of AA + gallic acid (GA) and AA + chlorogenic acid (CHA) mixtures at 476.19 μM increase 1.95 and 2.35 times respectively comparing with the sum of photocurrents of AA and GA or CHA. It is found that the synergistic effect is mainly depending on the fact that AA with the low redox potential (0.246 V vs NHE) can reduce other antioxidants radical to promote regeneration, improving the overall antioxidant performance. Moreover, it is proved that the greater redox potential of antioxidants, the more obvious the synergistic effect. In addition, the sensor was used to real sample assay, which provides available information towards food nutrition analysis, health products design and quality inspection.
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http://dx.doi.org/10.1016/j.bios.2021.112978DOI Listing
April 2021

Profiles of Childhood Maltreatment: Associations with Sexual Risk Behavior during Adolescence in a Sample of Racial/Ethnic Minority Girls.

Child Dev 2021 Jan 14. Epub 2021 Jan 14.

Mount Sinai Adolescent Health Center.

This study examines associations between childhood maltreatment and developmental trajectories of sexual risk behaviors (SRBs) in a sample of 882 sexually active adolescent girls, predominantly Hispanic or Black, assessed every 6 months between 13 and 23 years. Latent profile analyses revealed four distinct maltreatment profiles: Low Maltreatment (76%), Moderate Emotional Neglect Only (15%), Severe Physical/Emotional Abuse (3%), and Severe Sexual Abuse (6%). Multilevel growth analyses showed the Moderate Emotional Neglect Only and Severe Sexual Abuse profiles exhibited more SRBs starting in late adolescence, and the Severe Sexual Abuse profile also exhibited a faster increase than the Low Maltreatment profile. Understanding heterogeneity within maltreated populations may have important implications for healthy sexual development.
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http://dx.doi.org/10.1111/cdev.13498DOI Listing
January 2021

Contrasting effects of the aboveground litter of native Phragmites australis and invasive Spartina alterniflora on nitrification and denitrification.

Sci Total Environ 2021 Apr 24;764:144283. Epub 2020 Dec 24.

Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Coastal Ecosystems Research Station of Yangtze River Estuary, Institute of Biodiversity Science and Institute of Eco-Chongming, School of Life Sciences, Fudan University, Shanghai 200433, China. Electronic address:

Aboveground litter inputs from plants are among the most important pathways for carbon and nutrient fluxes to the soil. Previous studies on the effects of aboveground litter from invasive plants on ecosystem processes have primarily focused on biogeochemical cycling processes such as C and N mineralization, whereas the effects of aboveground litter from invasive plants on nitrogen removal processes are not well understood. In this study, the effects of the aboveground litter of native Phragmites australis and exotic Spartina alterniflora on soil nitrification and denitrification were compared. Results showed that the removal of the aboveground litter of both species had no effect on nitrification or denitrification in the early growth phase. However, after aboveground litter removal in the late growth phase, nitrification and denitrification in the P. australis stands decreased by 41.18% and 25.11%, respectively, whereas no such changes were observed in the S. alterniflora stands. These results indicate that the impacts of aboveground litter on nitrification and denitrification are species-specific. The aboveground litter from indigenous P. australis affected the SOC content and then indirectly affected nitrification or denitrification, and these effects were clearer in the late growth phase. Although other studies have reported that the invasive S. alterniflora have strong impacts on nitrogen removal processes, our study showed that the aboveground litter from S. alterniflora did not alter nitrification or denitrification, which indicates that other pathways may play important roles in nitrogen removal processes than its aboveground litter does.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144283DOI Listing
April 2021

Molecularly imprinted photo-electrochemical sensor for hemoglobin detection based on titanium dioxide nanotube arrays loaded with CdS quantum dots.

Talanta 2021 Mar 28;224:121924. Epub 2020 Nov 28.

Center for Advanced Analytical Science, School of Chemistry and Chemical Engineering c/o School of Civil Engineering, Guangzhou University, Guangzhou, 510006, China; State Key Laboratory of Electroanalytical Chemistry, c/o Engineering Laboratory for Modern Analytical Techniques, CAS Center for Excellence in Nanoscience, Changchun Institute of Applied Chemistry, Changchun, 130022, China; University of Chinese Academy of Sciences, Beijing, 100039, China.

A novel molecularly imprinted photo-electrochemical sensor based on CdS/TiO nanocomposites was constructed for precisely detection of hemoglobin under visible light irradiation. CdS quantum dots were decorated on the surface of TiO nanorod arrays to form a heterojunction, which could enhance the charge-transfer efficiency for visible light and further increase the photo-generated current of the sensor. The molecularly imprinted polymer film assembled by dopamine monomer had achieved excellent performance for specifically binding with human hemoglobin. The hemoglobin bound on the sensor could catalyze the oxidation reaction of 4-chloro-1-naphthol by HO, generating insoluble product on the sensor surface and triggering an obviously decrease on photocurrent. The molecularly imprinted photo-electrochemical sensor exhibited excellent sensitivity, selectivity and stability for the detection of human hemoglobin. The sensor had a linear range from 0.01 to 100 ng mL with a detection limit of 0.53 pg/mL (S/N = 3). Furthermore, the sensor was successfully applied on the analysis of human hemoglobin in the urine samples.
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http://dx.doi.org/10.1016/j.talanta.2020.121924DOI Listing
March 2021

Review of the formation and influencing factors of food-derived glycated lipids.

Crit Rev Food Sci Nutr 2020 Dec 29:1-16. Epub 2020 Dec 29.

Center for Advanced Analytical Science, School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, China.

Glycated lipids are formed by a Maillard reaction between the aldehyde group of a reducing sugar with the free amino group of an amino-lipid. The formation and accumulation of glycated lipids are closely related to the prognosis of diabetes, vascular disease, and cancer. However, it is not clear whether food-derived glycated lipids pose a direct threat to the human body. In this review, potentially harmful effect, distribution, formation environment and mechanism, and determination and inhibitory methods of glycated lipids are presented. Future research directions for the study of food-derived glycated lipids include: (1) understanding their digestion, absorption, and metabolism in the human body; (2) expanding the available database for associated risk assessment; (3) relating their formation mechanism to food production processes; (4) revealing the formation mechanism of food-derived glycated lipids; (5) developing rapid, reliable, and inexpensive determination methods for the compounds in different foods; and (6) seeking effective inhibitors. This review will contribute to the final control of food-derived glycated lipids.
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http://dx.doi.org/10.1080/10408398.2020.1867052DOI Listing
December 2020

Enhancing transient protein expression in HEK-293 cells by briefly exposing the culture to DMSO.

J Neurosci Methods 2021 Feb 24;350:109058. Epub 2020 Dec 24.

Department of Chemistry and Center for Neuroscience Research, University at Albany, State University of New York, Albany, NY, 12222, United States. Electronic address:

Background: Transient expression of proteins in mammalian cells is a key technique for many functional and structural studies of human and higher eukaryotic genes as well as for the production of recombinant protein therapeutics. Maximizing the expression efficiency to achieve a higher expression yield is desirable and may be even critical when, for instance, an expressed protein must be characterized at the single-cell level.

New Methods: Our goal was to develop a simple method by which protein expression yield in human embryonic kidney (HEK)-293 cells could be enhanced with a brief treatment of dimethyl sulfoxide (DMSO) solution.

Results: By expressing green fluorescent protein (GFP) as a reporter protein using the calcium phosphate transfection method and imaging a large population of cells, we found that a 5-min exposure of 10 % DMSO to HEK-293 cells, 4 h after transfection of the protein of interest, leads to ∼1.6-fold increase in the expression yield without causing any appreciable cytotoxicity. By expressing an α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and separately a kainate receptor in HEK-293 cells and measuring glutamate-induced whole-cell current response, we also found that such a brief DMSO treatment did not affect channel activity.

Conclusion: This method is simple, efficient and inexpensive to use for enhancing transient transfection yield in HEK-293 cells.
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http://dx.doi.org/10.1016/j.jneumeth.2020.109058DOI Listing
February 2021

CdS/TiO Nanocomposite-Based Photoelectrochemical Sensor for a Sensitive Determination of Nitrite in Principle of Etching Reaction.

Anal Chem 2021 01 15;93(2):820-827. Epub 2020 Dec 15.

Center for Advanced Analytical Science, School of Chemistry and Chemical Engineering c/o School of Civil Engineering, Guangzhou University, Guangzhou 510006, P. R. China.

The CdS/TiO nanocomposite (NC) photoelectrochemical (PEC) sensor was constructed based on a new sensing strategy for nitrite assay. The CdS etching process caused by nitrite-in-acid solution was confirmed and applied to nitrite sensing. The CdS etching phenomenon occurring on the sensor led to an obvious reduction in the photocurrent response under visible-light irradiation, which responded to the nitrite concentration. The CdS/TiO NC-based PEC sensor exhibited excellent performance on nitrite detection. The linear range for nitrite determination was from 1-100 and 100-500 μM, and the sensitivity of the PEC sensor was 2.91 and 0.186 μA μM cm, respectively. The detection limit of the sensor was 0.56 μM (S/N = 3). In addition, the PEC sensor was also equipped with advantages such as good selectivity, excellent stability, low background, and recyclability. Satisfying results were obtained for the nitrite assay in real samples by such a PEC sensor. In summary, this work contributed a fresh idea to precisely determinate nitrite through PEC sensing.
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http://dx.doi.org/10.1021/acs.analchem.0c03315DOI Listing
January 2021

Psychomotor development and attention problems caused by a splicing variant of CNKSR2.

BMC Med Genomics 2020 12 9;13(1):182. Epub 2020 Dec 9.

Department of Medical Genetics and Molecular Diagnostic Laboratory, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, People's Republic of China.

Background: Mutations in CNKSR2 have been described in patients with neurodevelopmental disorders characterized by childhood epilepsy, language deficits, and attention problems. The encoded protein plays an important role in synaptic function.

Case Presentation: Whole-exome sequencing was applied to detect pathogenic variants in a patient with clinical symptoms of psychomotor development, attention deficit, poor logical thinking ability, and an introverted personality, but without epilepsy or any significant electroencephalogram changes. Genetic study revealed a splicing mutation (c.1904 + 1G > A) and RT-PCR revealed aberrant splicing of exon 16, leading to a reading-frame shift and a truncated protein in the PH domain.

Conclusions: This is the first report of a splicing variant of CNKSR2, and the unique clinical features of this pedigree will help extend our understanding of the genetic and phenotypic spectra of CNKSR2-related disorders.
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http://dx.doi.org/10.1186/s12920-020-00844-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727132PMC
December 2020

The inhibitory effects of sesamol and sesamolin on the glycidyl esters formation during deodorization of vegetables oils.

J Sci Food Agric 2020 Dec 4. Epub 2020 Dec 4.

Center for Advanced Analytical Science, School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, China.

Background: Glycidyl esters (GEs) have attracted worldwide attention for their potential harm to human health. The GEs in edible oils mainly form during the deodorization of the oil refining processes. We used sesamol and sesamolin to inhibit the formation of GEs in model corn oil (MCO), model palm oil (MPO) and model rice bran oil (MRO) during a deodorization process.

Results: The results showed that, in the three model oils, the total GE content was in the following order from highest to lowest: MRO (1437.98 μg kg ) > MPO (388.64 μg kg ) > MCO (314.81 μg kg ). The inhibitory effect of the three antioxidants on the formation of GEs in the MCO was in the following order from strongest to weakest: tert-butylhydroquinone (TBHQ) > sesamol > sesamolin.

Conclusion: When the mass percentage of sesamol was 0.05%, its inhibition percentage on GEs was close to the inhibition percentage of 0.02% added TBHQ. The present study provides a foundation for understanding how to inhibit the formation of GEs in oils by adding sesamol during the deodorization process.
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http://dx.doi.org/10.1002/jsfa.10989DOI Listing
December 2020

Electrochemically Controlled ATRP for Cleavage-Based Electrochemical Detection of the Prostate-Specific Antigen at Femtomolar Level Concentrations.

Anal Chem 2020 12 22;92(24):15982-15988. Epub 2020 Nov 22.

Center for Advanced Analytical Science, School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, P. R. China.

As a single-chain glycoprotein with endopeptidase activity, the prostate-specific antigen (PSA) is valuable as an informative serum marker in diagnosing, staging, and prognosis of prostate cancer. In this report, an electrochemical biosensor based on the target-induced cleavage of a specific peptide substrate (PSA peptide) is designed for the highly selective detection of PSA at the femtomolar level, using electrochemically controlled atom transfer radical polymerization (eATRP) as a method for signal amplification. The PSA peptides, without free carboxyl sites, are attached to the gold surface via the -terminal cysteine residue. The target-induced cleavage of PSA peptides results in the generation of carboxyl sites, to which the alkyl halide initiator α-bromophenylacetic acid (BPAA) is linked via the Zr(IV) linkers. Subsequently, the potentiostatic eATRP of ferrocenylmethyl methacrylate (FcMMA, as the monomer) leads to the surface-initiated grafting of high-density ferrocenyl polymers. As a result, a large amount of Fc redox tags can be recruited for signal amplification, through which the limit of detection (LOD) for PSA can be down to 3.2 fM. As the recognition element, the PSA peptide is easy to synthesize, chemically and thermally stable, and low-cost. Without the necessity of enzyme or nanoparticle labels, the eATRP-based amplification method is easy to operate and low-cost. Results also show that the cleavage-based electrochemical PSA biosensor is highly selective and applicable to PSA detection in complex biological samples. In view of these merits, the integration of the eATRP-based amplification method into cleavage-based recognition is believed to hold great promise for the electrochemical detection of PSA in clinical applications.
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http://dx.doi.org/10.1021/acs.analchem.0c03467DOI Listing
December 2020

Person Re-Identification With Reinforced Attribute Attention Selection.

IEEE Trans Image Process 2021 25;30:603-616. Epub 2020 Nov 25.

Person re-identification (Re-ID) aims to match pedestrian images across various scenes in video surveillance. There are a few works using attribute information to boost Re-ID performance. Specifically, those methods leverage attribute information to boost Re-ID performance by introducing auxiliary tasks like verifying the image level attribute information of two pedestrian images or recognizing identity level attributes. Identity level attribute annotations cost less manpower and are well-fitted for person re-identification task compared with image-level attribute annotations. However, the identity attribute information may be very noisy due to incorrect attribute annotation or lack of discriminativeness to distinguish different persons, which is probably unhelpful for the Re-ID task. In this paper, we propose a novel Attribute Attentional Block (AAB), which can be integrated into any backbone network or framework. Our AAB adopts reinforcement learning to drop noisy attributes based on our designed reward and then utilizes aggregated attribute attention of the remaining attributes to facilitate the Re-ID task. Experimental results demonstrate that our proposed method achieves state-of-the-art results on three benchmark datasets.
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http://dx.doi.org/10.1109/TIP.2020.3036762DOI Listing
November 2020

LL-37-Induced Autophagy Contributed to the Elimination of Live Internalized in Keratinocytes.

Front Cell Infect Microbiol 2020 15;10:561761. Epub 2020 Oct 15.

Department of Periodontology, School and Hospital of Stomatology, China Medical University, Shenyang, China.

(), one of the most important pathogens of periodontitis, is closely associated with the aggravation and recurrence of periodontitis and systemic diseases. Antibacterial peptide LL-37, transcribed from the cathelicidin antimicrobial peptide () gene, exhibits a broad spectrum of antibacterial activity and regulates the immune system. In this study, we demonstrated that LL-37 reduced the number of live (ATCC 33277) in HaCaT cells in a dose-dependent manner via an antibiotic-protection assay. LL-37 promoted autophagy of HaCaT cells internalized with . Inhibition of autophagy with 3-methyladenine (3-MA) weakened the inhibitory effect of LL-37 on the number of intracellular . A cluster of orthologous groups (COGs) and a gene ontology (GO) functional analysis were used to individually assign 65 (10%) differentially expressed genes (DEGs) to an "Intracellular trafficking, secretion, and vesicular transport" cluster and 306 (47.08%) DEGs to metabolic processes including autophagy. Autophagy-related genes, a tripartite motif-containing 22 (), and lysosomal-associated membrane protein 3 () were identified as potentially involved in LL-37-induced autophagy. Finally, bioinformatics software was utilized to construct and predict the protein-protein interaction (PPI) network of CAMP-TRIM22/LAMP3-Autophagy. The findings indicated that LL-37 can reduce the quantity of live internalized in HaCaT cells by promoting autophagy in these cells. The transcriptome sequencing and analysis also revealed the potential molecular pathway of LL-37-induced autophagy.
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http://dx.doi.org/10.3389/fcimb.2020.561761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593823PMC
October 2020

Silencing of long non‑coding RNA HRIM protects against myocardial ischemia/reperfusion injury via inhibition of NF‑κB signaling.

Mol Med Rep 2020 Dec 14;22(6):5454-5462. Epub 2020 Oct 14.

Cadre Health Department, Qingdao Municipal Hospital, Qingdao, Shandong 266011, P.R. China.

Myocardial ischemia/reperfusion injury (MI/RI) following cardiac surgery is a leading cause of morbidity and mortality worldwide. The aim of the present study was to investigate the role of long non‑coding RNA hypoxia/reoxygenation injury‑related factor in myocytes (HRIM) on cardiac function following MI/RI. After establishing an MI/RI model, hemodynamic indices were detected via transthoracic echocardiography. The proliferative and apoptotic capacities of H9C2 cells subjected to oxygen‑glucose deprivation/reoxygenation were detected via Cell Counting Kit‑8 assay and flow cytometry, respectively. TNF‑α, IL‑1β, IL‑6, lactate dehydrogenase (LDH) and creatine kinase (CK) levels were measured via ELISA. The expression levels of NF‑κB‑associated proteins were detected via western blotting. The expression levels of HRIM were increased in the myocardial tissue of MI/RI rats and H9C2 cells. The infarct size was significantly increased following induction of MI/RI. Moreover, increased HRIM expression levels suppressed hemodynamics in MI/RI rats. Knockdown of HRIM increased cell proliferation and decreased apoptosis as well as the protein levels of phosphorylated (p)‑NF‑κB p65/NF‑κB p65, p‑IkBα/IkBα, TNF‑α, IL‑1β, IL‑6, LDH and CK in H9C2 cells; however, these effects were attenuated via activation of NF‑κB signaling. Silencing of HRIM ameliorated MI/RI injury and alleviated inflammation via inactivating the NF‑κB signaling pathway.
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http://dx.doi.org/10.3892/mmr.2020.11597DOI Listing
December 2020

Carbon Nitride Quantum Dots Enhancing the Anodic Electrochemiluminescence of Ruthenium(II) Tris(2,2'-bipyridyl) via Inhibiting the Oxygen Evolution Reaction.

Anal Chem 2020 12 10;92(23):15352-15360. Epub 2020 Nov 10.

Engineering Laboratory for Modern Analytical Techniques, C/o State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.

Although electrochemiluminescence (ECL) has been developed significantly in the past few decades, ECL efficiency in aqueous solutions remains quite low. Determination of the energy losses and development of new ECL-enhancing strategies are still of great value. In this work, we discovered a detrimental nonradiation relaxation pathway by a concurrent oxygen evolution reaction (OER) process in a well-known ruthenium(II) tris(2,2'-bipyridyl) (Ru(bpy)) aqueous ECL system due to similar surface-sensitive characteristics, and for the first time, a chemical strategy was developed by which carbon nitride quantum dots (CNQDs) could inhibit the surface OER process, alleviate the energy losses by nonradiation relaxation, and enhance the anodic ECL of Ru(bpy). In the Ru(bpy)/CNQD system, CNQDs could enhance the anodic ECL of Ru(bpy) in a nitrogen stream (10-fold) and ambient air (161-fold). The luminous and nitrogen-rich CNQDs were also confirmed not to serve as ECL luminophores, anodic coreactants, or donor/acceptors in ECL. The coreactant-free Ru(bpy)/CNQD system possesses several advantages over the common coreactant ECL system, such as low dosage (100 μg/mL CNQDs), favorable regeneration capacity, etc. As an example, ECL on-off detection of dopamine utilizing the Ru(bpy)/CNQD system was also developed to show prospects in ECL sensing. Besides, CNQDs were introduced into the classical Ru(bpy)/CO coreactant ECL system, leading to suppressed OER and improved ECL signal. Overall, the proposed new ECL-enhancing strategy is promising for applicable ECL sensing, could be extended to other ECL luminophores with high oxidation potential, and enables an in-depth understanding of the ECL process and mechanism.
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http://dx.doi.org/10.1021/acs.analchem.0c02568DOI Listing
December 2020

A novel PIK3R1 mutation of SHORT syndrome in a Chinese female with diffuse thyroid disease: a case report and review of literature.

BMC Med Genet 2020 10 31;21(1):215. Epub 2020 Oct 31.

Department of Endocrinology and Metabolism, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: SHORT syndrome is a rare genetic disease named with the acronyms of short stature, hyper-extensibility of joints, ocular depression, Rieger anomaly and teething delay. It is inherited in an autosomal dominant manner confirmed by the identification of heterozygous mutations in PIK3R1. This study hereby presents a 15-year-old female with intrauterine growth restriction, short stature, teething delay, characteristic facial gestalts who was identified a novel de novo nonsense mutation in PIK3R1.

Case Presentation: The proband was admitted to our department due to irregular menstrual cycle and hirsutism with short stature, who had a history of intrauterine growth restriction and presented with short stature, teething delay, characteristic facial gestalts, hirsutism, and thyroid disease. Whole-exome sequencing and Sanger sequencing revealed c.1960C > T, a novel de novo nonsense mutation, leading to the termination of protein translation (p. Gln654*).

Conclusions: This is the first case report of SHORT syndrome complicated with thyroid disease in China, identifying a novel de novo heterozygous nonsense mutation in PIK3R1 gene (p. Gln654*). The phenotypes are mildly different from other cases previously described in the literature, in which our patient presents with lipoatrophy, facial feature, and first reported thyroid disease. Thyroid disease may be a new clinical symptom of patients with SHORT syndrome.
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http://dx.doi.org/10.1186/s12881-020-01146-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7603772PMC
October 2020

Vitamin D decreases Porphyromonas gingivalis internalized into macrophages by promoting autophagy.

Oral Dis 2020 Oct 24. Epub 2020 Oct 24.

Liaoning Provincial Key Laboratory of Oral Diseases, Department of Periodontology, School and Hospital of Stomatology, China Medical University, Shenyang, China.

Objectives: This paper aims to study the effect of the active form of vitamin D (calcitriol) on the internalized Porphyromonas gingivalis in macrophages and to assess the role of autophagy during this process.

Materials And Methods: Quantitative RT-PCR and bacteria culture were used to quantify live P. gingivalis internalized into U937-derived macrophages. Western blot assays were performed to detect the effect of P. gingivalis and calcitriol on autophagy in macrophages. Transmission electron microscope was used to observe the effect of calcitriol on the status of internalized P. gingivalis. Colocalization of P. gingivalis with the autophagosome and lysosome markers was observed by confocal laser scanning microscopy.

Results: Calcitriol caused a dose-dependent decrease in live P. gingivalis numbers and promoted both the endogenous and P. gingivalis-induced autophagy in macrophages. Calcitriol significantly promoted the destruction of P. gingivalis and the colocalization of P. gingivalis with autophagosome and lysosome markers. Conversely, with 3-MA, live P. gingivalis numbers in macrophages increased significantly and inhibition effect of calcitriol on the number of live P. gingivalis was attenuated.

Conclusion: In U937-derived macrophages, calcitriol may promote colocalization of P. gingivalis with autophagosomes and lysosomes, namely autophagy process, to degrade live P. gingivalis.
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http://dx.doi.org/10.1111/odi.13696DOI Listing
October 2020

TRPS1 mutation detection in Chinese patients with Tricho-rhino-phalangeal syndrome and identification of four novel mutations.

Mol Genet Genomic Med 2020 10 10;8(10):e1417. Epub 2020 Aug 10.

Department of Medical Genetics and Molecular Diagnostic Laboratory, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.

Background: Tricho-rhino-phalangeal syndrome (TRPS) is a rare autosomal dominant disorder characterized by craniofacial and skeletal malformations including short stature, thin scalp hair, sparse lateral eyebrows, a pear-shaped nose, and cone-shaped epiphyses. This condition is caused by haploinsufficiency or dominant-negative effect of the TRPS1 gene.

Methods: In this study, we analyzed the clinical and genetic data of five unrelated TRPS patients. They were suspected of having TRPS on the basis of clinical and radiological features including typical hair and facial features, as well as varying degrees of skeletal abnormalities. Next-generation sequencing was performed to identify variants of the TRPS1 gene in the five patients.

Results: In patient 1, we found a novel mutation at c.1338C>A (p.Tyr446*) (de novo). Patient 2 had a novel phenotype of hydrocephaly and Arnold-Chiari syndrome and we also found a maternally inherited novel mutation at c.2657C>A (p.Ser886*). Patient 3 had a de novo novel mutation at c.2726G>C (p.Cys909Ser) leading to more severe phenotypes. Patient 4 had a paternally inherited known mutation at c.2762G>A (p.Arg921Gln). Patient 5 with a novel phenotype of hepatopathy had a novel deletion at [GRCh37] del(8)(q23.3-q24.11) chr8:g.116,420,724-119,124,058 (over 2,700 kb). In addition, the patient 3 who harboring missense variants in the GATA binding domain of TRPS1 showed more severe craniofacial and skeletal phenotypes.

Conclusions: We describe four novel mutations and two novel phenotypes in five patients. The mutational and phenotypic spectrum of TRPS is broadened by our study on TRPS mutations. Our results reveal the significance of molecular analysis of TRPS1 for improving the clinical diagnosis of TRPS.
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http://dx.doi.org/10.1002/mgg3.1417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7549555PMC
October 2020

Clinicopathologic features of invasive breast carcinoma with dermatopathic lymphadenitis: a retrospective analysis.

Int J Clin Exp Pathol 2020 1;13(9):2289-2296. Epub 2020 Sep 1.

Department of Pathology, Zhongnan Hospital of Wuhan University Wuhan, People's Republic of China.

Purpose: To understand and explore the clinical manifestations, pathologic features, immunohistochemical analysis, treatment, and prognosis of dermatopathic lymphadenitis (DPL) and review the literature.

Methods: Lymph node specimens were collected from 10 patients with DPL at Zhongnan Hospital of Wuhan University from 2016 to 2019. We identified 3 patients with invasive breast carcinoma with DPL. We examined 41 axillary lymph nodes from the 3 patients, and only 3 lymph nodes showed DPL. Samples were analyzed by paraffin embedding and sectioning and hematoxylin-eosin and immunohistochemical staining.

Results: The lymph node cortex and medulla were generally maintained, and lymphoid follicles were atrophic. Irregularly shaped and distributed patches in the subcapsular lymphatic sinusoid and paracortical areas palely stained were observed. Melanin was deposited in the cytoplasm and intercellular spaces. Large cytoplasm-rich cells were also found in the tissue. The boundaries between the cells were unclear, and cytoplasm was bright. Immunohistochemistry of all DPL cases showed CD1a (+) and S-100 (+); some cases were Langerin positive.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7539859PMC
September 2020