Publications by authors named "Li Ma"

2,836 Publications

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LncRNA-p21 suppresses cell proliferation and induces apoptosis in gastric cancer by sponging miR-514b-3p and up-regulating ARHGEF9 expression.

Biol Chem 2022 Aug 11. Epub 2022 Aug 11.

Department of Oncology, The Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an 710004, Shaanxi, China.

The long non-coding RNA p21 (lncRNA-p21) was a tumor suppressor gene in most cancer types including gastric cancer (GC). We aimed to identify a specific lncRNA-p21-involved pathway in regulating the proliferation and apoptosis of GC cells. A lower lncRNA-p21 expression in tumors was associated with advanced disease stage and predicted worse survival of GC patients. LncRNA-p21 overexpression in GC cell line somatic gastric cancer (SGC)-7901 and human gastric cancer (HGC)-27 suppressed cell proliferation and enhanced apoptosis, while lncRNA-p21 knockdown caused the opposite effects. Through bioinformatics analysis and luciferase-based reporter assays, we identified miR-514b-3p as a sponge target of lncRNA-p21. Cdc42 guanine nucleotide exchange factor 9 (ARHGEF9), functioned as a tumor suppress factor in GC, was found as the downstream target of miR-514-3p, and their expressions were negatively correlated in GC tumor tissues. In addition, like lncRNA-p21 overexpression alone, miR-514-3p inactivation alone also led to decreased proliferation and increased apoptosis in SGC-7901 and HGC-27 cells, which were markedly attenuated by additional ARHGEF9 knockdown. Xenograft SGC-7901 cells with more lncRNA-p21 or ARHGEF9 expressions or with less miR-514-3p expression exhibited obviously slower growth than the control SGC-7901 cells in nude mice. Our study reveals a novel lncRNA-p21/miR-514b-3p/ARHGEF9 pathway that can be targeted for GC therapy.
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http://dx.doi.org/10.1515/hsz-2022-0153DOI Listing
August 2022

Bentall procedure for retrograde type A dissection after endovascular repair in type A aortic dissection: A case report.

Medicine (Baltimore) 2022 Aug;101(31):e29615

Department of Cardiovascular Surgery, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, China.

Rationale: The management of retrograde type A dissection (RTAD) after thoracic endovascular aortic repair (TEVAR) for type A aortic dissection (TAAD) has rarely been reported. We report the management of RTAD after TEVAR with in situ fenestration for TAAD.

Patient Concerns: A 59-year-old man with TAAD had undergone TEVAR with in situ fenestration 4 months prior to presenting to our emergency room complaining of acute chest and back pain. Computed tomography angiography showed RTAD starting from the proximal endograft and extending to the aortic root.

Diagnosis: The patient was diagnosed with RTAD.

Interventions: We performed only the Bentall procedure, and the patient did not require total arch replacement. We removed the bare spring of the proximal endograft and anastomosed the prosthetic graft with the endograft and the native ascending aortic wall.

Outcomes: The postoperative course was uneventful, and the patient remained asymptomatic for 3 years after surgery. Computed tomography angiography at the 3-year follow-up showed no perivalvular or anastomotic leakage.

Lessons: RTAD after TEVAR for TAAD was safely and effectively treated by anastomosing the prosthetic graft with the endograft and the native ascending aortic wall instead of total arch replacement.
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http://dx.doi.org/10.1097/MD.0000000000029615DOI Listing
August 2022

Efficient electrochemical degradation of X-GN dye wastewater using porous boron-doped diamond electrode.

Chemosphere 2022 Aug 5;307(Pt 2):135912. Epub 2022 Aug 5.

College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, PR China.

Surface porous Ti substrates were obtained by electrodeposition-hot melt-alkali etching. Porous-Ti/BDD and flat-Ti/BDD electrodes were prepared for comparative study. The results of SEM, Raman, and XRD analyses show that the BDD films of these two electrodes had good uniformity and stable quality. The electrochemical window (EW) and electrochemical-active surface area (EASA) of the porous-Ti/BDD electrode is as high as 4.21 V and 22.78 cm (11.39 cm/cm), respectively. Furthermore, the electrochemical catalytic performance and degradation mechanism of porous-Ti/BDD electrode as the anode were studied by the electrolysis of Active Orange dye X-GN (X-GN), and the optimal electrochemical degradation operating parameters were obtained. The results show that when the degradation time was 50 min, the X-GN was completely decolorized. The TOC removal rate reached 69.24%, and the energy consumption was 5.62 kWh m. The contribution rate of •OH and SO was calculated to be 91.40% and 1.26% by radical quenching experiments, respectively, indicating that the active substances in the degradation system were mainly •OH and SO. The high specific surface characteristics of porous-Ti/BDD electrode enhanced its electrochemical oxidation advantages, and it showed a high degradation efficiency and low energy consumption for the treatment of X-GN simulated wastewater.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135912DOI Listing
August 2022

Cold exposure-induced endoplasmic reticulum stress regulates autophagy through the SIRT2/FoxO1 signaling pathway.

J Cell Physiol 2022 Aug 8. Epub 2022 Aug 8.

National Experimental Teaching Demonstration Center of Animal Medicine Foundation, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China.

Cold is a factor affecting health in humans and animals. The liver, a major metabolic center, is highly susceptible to ambient air temperature. Recent studies have shown that endoplasmic reticulum (ER) stress is associated with the liver, and regulates the occurrence and development of liver injury and autophagy. However, the mechanism underlying the relationship between cold exposure and ER stress in the liver is not well understood. In this study, we investigated the effect of ER stress on liver autophagy and its mechanism under cold exposure. AML12 cells were treated with Tg to construct an ER stress model, and the level of autophagy increased. To further explore the mechanism through which ER stress regulates autophagy, we knocked down SIRT2 with shRNA in Tg-treated AML12 cells. Knockdown of SIRT2 significantly increased ER stress and autophagy, increased FoxO1 acetylation, and promoted its entry into the nucleus. To further verify the results of in vitro experiments, we exposed mice to 4°C for 3 h per day for 3 weeks to exacerbate the burden on the liver after cold exposure. Cold exposure damaged the structure and function of the liver and promoted the inflammatory response. It also activated ER stress and promoted autophagy. In addition, cold exposure inhibited the expression of SIRT2, promoted FoxO1 acetylation, and enhanced the interaction with autophagy. Our findings indicated that cold exposure induces liver damage, ER stress, and autophagy through the SIRT2/FoxO1 pathway. These findings suggest that SIRT2 may be a potential target for regulating health under cold exposure.
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http://dx.doi.org/10.1002/jcp.30856DOI Listing
August 2022

Transcriptomic Insights Into Root Development and Overwintering Transcriptional Memory of L. Grown in the Field.

Front Plant Sci 2022 22;13:900708. Epub 2022 Jul 22.

State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, China.

As the only overwintering oil crop in the north area of China, living through winter is the primary feature of winter rapeseed. Roots are the only survival organ during prolonged cold exposure during winter to guarantee flowering in spring. However, little is known about its root development and overwintering memory mechanism. In this study, root collar tissues (including the shoot apical meristem) of three winter rapeseed varieties with different cold resistance, i.e., Longyou-7 (strong cold tolerance), Tianyou-4 (middle cold tolerance), and Lenox (cold-sensitive), were sampled in the pre-winter period (S1), overwintering periods (S2-S5), and re-greening stage (S6), and were used to identify the root development and overwintering memory mechanisms and seek candidate overwintering memory genes by measuring root collar diameter and RNA sequencing. The results showed that the S1-S2 stages were the significant developmental stages of the roots as root collar diameter increased slowly in the S3-S5 stages, and the roots developed fast in the strong cold resistance variety than in the weak cold resistance variety. Subsequently, the RNA-seq analysis revealed that a total of 37,905, 45,102, and 39,276 differentially expressed genes (DEGs), compared to the S1 stage, were identified in Longyou-7, Tianyou-4, and Lenox, respectively. The function enrichment analysis showed that most of the DEGs are significantly involved in phenylpropanoid biosynthesis, plant hormone signal transduction, MAPK signaling pathway, starch and sucrose metabolism, photosynthesis, amino sugar and nucleotide sugar metabolism, and spliceosome, ribosome, proteasome, and protein processing in endoplasmic reticulum pathways. Furthermore, the phenylpropanoid biosynthesis and plant hormone signal transduction pathways were related to the difference in root development of the three varieties, DEGs involved in photosynthesis and carbohydrate metabolism processes may participate in overwintering memory of Longyou-7 and Tianyou-4, and the spliceosome pathway may contribute to the super winter resistance of Longyou-7. The transcription factor enrichment analysis showed that the family made up the majority in different stages and may play an important regulatory role in root development and overwintering memory. These results provide a comprehensive insight into winter rapeseed's complex overwintering memory mechanisms. The identified candidate overwintering memory genes may also serve as important genetic resources for breeding to further improve the cold resistance of winter rapeseed.
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http://dx.doi.org/10.3389/fpls.2022.900708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355659PMC
July 2022

Prognosis of adenoid cystic carcinoma in head and neck region treated with different regimens-A single-centre study.

Cancer Med 2022 Aug 7. Epub 2022 Aug 7.

Department of Oral and Maxillofacial Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.

Background: No study has evaluated the impact of regimen on recurrence, metastasis and survival in patients with adenoid cystic carcinoma (ACC). The present study aimed to compare the efficacy of radioactive seed implantation and other regimens in treating ACC, so as to investigate the clinical applicability of radioactive seed implantation and determine the indications for this regimen.

Methods: A total of 188 patients with ACC in oromaxillofacial region were allocated to four groups according to the treatment regimen: group 1 was treated with a combination of surgery and I seed therapy, group 2 with a combination of surgery and external radiotherapy, group 3 with surgery, whereas group 4 was untreated. The Kaplan-Meier method was used to assess the survival rates, and the Cox regression analyses were used to identify the associated prognostic factors.

Results: The overall survival rates of 188 patients and groups 1, 2, 3 and 4 were 85.7%, 75%, 68.2% and 37.5%, respectively. Cox regression analysis revealed that age, T stage, N stage and regimen were independent prognostic factors of survival. Amongst patients with primary ACC, the efficacy of radioactive seed implantation was higher in those with perineural invasion than in those without.

Conclusion: Patient age, T stage, N stage and regimen are independent prognostic factors of survival in patients with ACC. Patients treated with surgery combined with postoperative I seed radiotherapy have a higher overall survival rate, and those with perineural invasion are more suitable for radioactive seed implantation therapy.
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http://dx.doi.org/10.1002/cam4.5065DOI Listing
August 2022

Three pairs of surrogate redox partners comparison for Class I cytochrome P450 enzyme activity reconstitution.

Commun Biol 2022 Aug 6;5(1):791. Epub 2022 Aug 6.

State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, 266237, China.

Most P450s require redox partners for the electron transfer during catalysis. However, little information is available on cognate redox partners for P450s, which greatly limits P450 function exploration and practical application. Thus, the stategy of building various hybrid P450 catalytic systems with surrogate redox partner has often adopted to engineer P450 biocatalysts. In this study, we compare three pairs of frequently-used surrogate redox partner SelFdx1499/SelFdR0978, Adx/AdR and Pdx/PdR and in terms of their electron transfer properties. The three selected bacterial Class I P450s include PikC, P450sca-2 and CYP-sb21, which are responsible for production of high-value-added products. Here we show that SelFdx1499/SelFdR0978 is the most promising redox partner compared to Adx/AdR and Pdx/PdR. The results provide insights into the domination for P450-redox partner interactions in modulating the catalytic activity of P450s. This study not only produces a more active biocatalyst but also suggests a general chose for a universal reductase which would facilitate engineering of P450 catalyst.
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http://dx.doi.org/10.1038/s42003-022-03764-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357085PMC
August 2022

PGC-1 alpha regulates mitochondrial biogenesis to ameliorate hypoxia-inhibited cementoblast mineralization.

Ann N Y Acad Sci 2022 Aug 2. Epub 2022 Aug 2.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS) & Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School & Hospital of Stomatology, Wuhan University, Wuhan, China.

Hypoxia often occurs in inflammatory tissues, such as tissues affected by periodontitis and apical periodontitis lesions. Mitochondrial biogenesis can be disrupted in hypoxia. Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) is a core factor required for mitochondrial biogenesis. Cementoblasts are root surface lining cells that play an integral role in cementum formation. There is a dearth of research on the effect of hypoxia on cementoblasts and underlying mechanisms, particularly in relation to mitochondrial biogenesis during the hypoxic process. In this study, we found that the expression of hypoxia inducible factor-1α was elevated in apical periodontitis tissues in vivo. In contrast, periapical lesions exhibited a reduction of PGC-1α expression. For in vitro experiments, cobalt chloride (CoCl ) was used to induce hypoxia. We observed that CoCl -induced hypoxia suppressed the mineralization ability and mitochondrial biogenesis of cementoblasts, accompanied by abnormal mitochondria morphology. Furthermore, we found that CoCl blocked the p38 pathway, while it activated the Erk1/2 pathway, with the former upregulating the expression of PGC-1α, while the latter reversed the effects. Overall, our findings demonstrate that mitochondrial biogenesis, especially via PGC-1α, is impaired during cementogenesis in the context of CoCl -induced hypoxia, dependent on the mitogen-activated protein kinase signaling pathway.
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http://dx.doi.org/10.1111/nyas.14872DOI Listing
August 2022

M2 macrophages with inflammation tropism facilitate cementoblast mineralization.

J Periodontol 2022 Aug 1. Epub 2022 Aug 1.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS) & Key Laboratory of Oral Biomedicine Ministry of Education (KLOBME), School & Hospital of Stomatology, Wuhan University, Wuhan, China.

Background: Cementum regeneration was regarded as the critical goal for periodontal regeneration. M2 macrophage-based therapy was expected to be a promising strategy for it. However, little is known about the effects of M2 macrophages on cementoblast mineralization and tropism, especially under inflammation. Here we first investigated the crosstalk between M2 macrophages and porphyromonas gingivalis (P. gingivalis)-stimulated cementoblasts.

Materials And Methods: M2 macrophages were induced with interleukin (IL)-4, and identified. CC-chemokine ligand 2 (CCL2) expression and secretion of inflammatory cementoblasts was detected by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR), western blotting (WB), immunohistochemistry for the apical periodontitis (AP) mice, and enzyme-linked immunosorbent assay. Crystal violet staining was used to observe macrophage migration. Conditional medium (CM) and transwell coculture methods were applied to evaluate the effects of M2 macrophages on cementum mineralization with or without P. gingivalis, and to explore the mechanism. Mineralization-related markers and pathway-related proteins were measured by RT-qPCR and WB.

Results: M2 macrophages were identified successfully. We found an increase of CCL2 in cementoblasts and their supernatant. Also, higher CCL2 in cementoblasts was observed in AP model. Superior recruitment of M2 macrophages to supernatant from P. gingivalis-stimulated cementoblasts or CCL2-containing medium was verified. Moreover, CM2 and Trans-M2 showed better mineralization-accelerating and -rescuing effects when compared to their controls, and application of p38 inhibitor partially blocked the promotion.

Conclusions: Our study demonstrated the inflammation-targeting and mineralization-promoting effects of M2 macrophages on cementoblasts, which may provide evidences for M2 macrophage-based cementum regeneration. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/JPER.22-0048DOI Listing
August 2022

High expression of RRM2 mediated by non-coding RNAs correlates with poor prognosis and tumor immune infiltration of hepatocellular carcinoma.

Front Med (Lausanne) 2022 14;9:833301. Epub 2022 Jul 14.

Department of Oncology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Hepatocellular carcinoma (HCC) is known to have a poor prognosis. Accumulating evidence indicates that RRM2 plays a critical role in the occurrence and progression of multiple human cancers. However, the knowledge about RRM2 in HCC is still insufficient, and further research is needed. Here, we first analyzed the expression and prognosis of RRM2 using TCGA and GTEx data, and found that RRM2 may play a potential carcinogenic role in HCC. Then, through a series of comprehensive analysis, including expression analysis, correlation analysis or survival analysis, non-coding RNAs (ncRNAs) that regulate RRM2 overexpression were identified. Finally, MIR4435-2HG/CYTOR were observed to be the most promising upstream lncRNAs for the miR-125b-5p/RRM2 axis in HCC. In addition, RRM2 expression was significantly positively related to immune cell infiltration, immune cell biomarker or immune checkpoint expression in HCC. Altogether, the upregulation of RRM2 mediated by ncRNAs correlates with poor prognosis and tumor immune infiltration of HCC.
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http://dx.doi.org/10.3389/fmed.2022.833301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330188PMC
July 2022

Reliability of Evidence to Guide Decision-Making in the Use of Acupuncture for Postpartum Depression.

Front Public Health 2022 14;10:942595. Epub 2022 Jul 14.

Nanchang Hongdu Hospital of Traditional Chinese Medicine, Nanchang, China.

Background: There is conflicting evidence on the effectiveness of acupuncture in the treatment of postpartum depression (PPD). This study aimed to assess previous systematic reviews/meta-analyses (SRs/MAs) on the effectiveness of acupuncture to treat PPD.

Method: SRs/MAs regarding the use of acupuncture for PPD were identified from the establishment of digital databases to November 2021. The Assessing the Methodological Quality of Systematic Reviews 2 (AMSTAR-2) was applied to evaluate the methodological quality of included SRs/MAs. The Grades of Recommendations, Assessment, Development and Evaluation (GRADE) was utilized to evaluate the evidence quality for outcomes of interest.

Results: Six studies that conducted quantitative syntheses were included. According to AMSTAR-2, the methodological quality of these SRs/MAs was critically low owing to limitations of items 2, 4, and 7. According to GRADE, no study included high-quality evidence and most studies included low-quality evidence.

Conclusions: Acupuncture m be beneficial for PPD, however, due to limitations of current evidence and inconsistent findings, further studies are needed to provide stronger evidence to draw definitive conclusions.
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http://dx.doi.org/10.3389/fpubh.2022.942595DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329701PMC
August 2022

Investigation of Pyrolysis and Mild Oxidation Characteristics of Tar-Rich Coal via Thermogravimetric Experiments.

ACS Omega 2022 Jul 13;7(29):25613-25624. Epub 2022 Jul 13.

State Key Laboratory of Multiphase Flow in Power Engineering, School of Energy and Power Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

Tar-rich coal has the potential to substitute the supply of oil-gas resources, which is abundant in China. The effective conversion of tar-rich coal into oil-gas products can promote coal utilization, reduce resource wastage, alleviate environmental pollution, and benefit carbon neutrality. Nevertheless, less work, if any, has been performed on the pyrolysis and mild oxidation behaviors of tar-rich coal in Northwestern China. The influences of limited oxygen addition and an extremely low heating rate on the micromorphology of the residual semi-coke are yet to be fully understood. Here, an experimental study on the pyrolysis and mild oxidation characteristics of tar-rich coal was conducted by the thermogravimetric analysis method, with further elucidation of the physical-chemical properties of the residual semi-coke. Experimental results show that an increase in the ultimate temperature of pyrolysis leads to a decline in the residue mass, while the mass loss from 500 to 550 °C presents the maximum elevation. Volatile matter is inclined to discharge from a certain direction, and the pores formed in various directions hold different possibilities. The organic components undergo both pyrolysis and slow oxidation with limited oxygen in the heating medium. Compared with an inert atmosphere, the mass loss under conditions of a small amount of O is brought forward but prolonged. Compared with a N atmosphere, the oxidation reactions of tar-rich coal are weakened in the presence of CO. A large decrease in the heating rate exerts an unfavorable effect on the production of total volatiles. An extremely low heating rate possibly brings about a change in the mechanism of chemical bond cracking during pyrolysis. More pores can be yielded in tar-rich coal with an increase in the heating rate, and the morphology of the residual semi-coke after pyrolysis is susceptible to the heating rate. The present study offers an improved understanding of the pyrolysis characteristics of tar-rich coal as well as insights into the efficient utilization of tar-rich coal.
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http://dx.doi.org/10.1021/acsomega.2c02786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330078PMC
July 2022

A Chromosome Level Genome Assembly of a Winter Turnip Rape (.) to Explore the Genetic Basis of Cold Tolerance.

Front Plant Sci 2022 15;13:936958. Epub 2022 Jul 15.

College of Agronomy, Gansu Agricultural University, Lanzhou, China.

Winter rapeseed (.) is an important overwintering oilseed crop that is widely planted in northwest China and suffers chronic low temperatures in winter. So the cold stress becomes one of the major constraints that limit its production. The currently existing genomes limit the understanding of the cold-tolerant genetic basis of rapeseed. Here we assembled a high-quality long-read genome of "Longyou-7" cultivar, which has a cold-tolerant phenotype, and constructed a graph-based pan-genome to detect the structural variations within homologs of currently reported cold-tolerant related genes in the "Longyou-7" genome, which provides an additional elucidation of the cold-tolerant genetic basis of "Longyou-7" cultivar and promotes the development of cold-tolerant breeding in .
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http://dx.doi.org/10.3389/fpls.2022.936958DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9335200PMC
July 2022

Topical 5-aminolevulinic acid photodynamic therapy for cervical high-grade squamous intraepithelial lesions.

Photodiagnosis Photodyn Ther 2022 Jul 26;39:103037. Epub 2022 Jul 26.

Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, No.600 Yishan Road, Shanghai 200233, China. Electronic address:

Objective: To evaluate the efficacy of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) in the treatment of high-grade squamous intraepithelial lesions of the cervix (HSIL).

Methods: This retrospective study included 22 female patients with histologically confirmed HSIL and high-risk human papillomavirus (HR-HPV) infections. Patients were treated with ALA-PDT once a week for a total of 6 times. All patients had a follow-up period of 3 months and 6 months. The assessment of effectiveness of ALA-PDT was performed by ThinPrep cytology test (TCT), HPV DNA assay, HPV E6/E7 mRNA examination, colposcopy, biopsy, and immunohistochemistry detection.

Results: Three months after 5-ALA PDT, the histologic disappearance rate was 81.82% (18/22), while the HPV clearance rate was 54.55% (12/22). At the 6 months checkpoint, the HSIL disappearance rate was 90.91% (20/22) and the HPV clearance rate was 86.36% (19/22). Before PDT, 68.18% of patients (15/22) were confirmed with TCT abnormalities, while only one patient (1/22, 4.55%) was abnormal in the TCT test at 6 months checkpoint. All participants were found HPV E6/E7 mRNA positive initially, while the HPV E6/E7 mRNA negative rate was 90.91% (20/22) at 6 months checkpoint. Additionally, we found a significant difference of the expression of CD4+ and CD8+ T cells and HPV E6 and E7 proteins before ALA-PDT and at 3 months follow-up (P < 0.01). No severe side effects were seen.

Conclusions: Topical 5-ALA PDT is an effective treatment for cervical HSIL with HR-HPV infection.
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http://dx.doi.org/10.1016/j.pdpdt.2022.103037DOI Listing
July 2022

The determination method of total polyphenol in tea and substitute tea based on [Ag(HIO6)2]5--luminol chemiluminescence system.

J AOAC Int 2022 Jul 29. Epub 2022 Jul 29.

Hebei Key Laboratory of Environment and Human Health, School of Public Health, Hebei Medical University, Shijiazhuang, 050017 China.

Background: Scientific, accurate and rapid detection of the composition and content of tea polyphenols is an important basis for their rational use and giving full play to their physiological effect. The spectrophotometric assays for total polyphenols were poor selectivity. Therefore, there is a need to develop a simple and reliable method for the determination of the total polyphenolic level in tea products.

Objective: The aim of this research was to develop a flow injection chemiluminescence (FI-CL) method based on Ag(III)-luminol system for the total polyphenols content analysis of tea and substitute tea.

Methods: Through Box-Behnken experimental design, we selected the optimum determination condition. The Ag (III) concentration was 5 × 10-5 mol/L, and the luminol concentration was 3 × 10-7 mol/L (including 0.15 mol/L NaOH).The peristaltic pump is 25 r/min and the photomultiplier voltage is 600v. Sample extracts were diluted 100,000 times for the FI-CL assay.

Results: Under optimal conditions, CL intensities were proportional to total polyphenols content(in terms of gallic acid concentrations) in the range of 0.1∼100 μg/L.The LOD and LOQ were 0.03 μg/L and 0.1 μg/L. The recovery values were in the range of 86.3-111.0% with RSD of 1.04∼2.62%. The polyphenolic content of 12 teas and 6 substitute teas were determined, and the results of the developed method and Folin-Ciocalteu method were highly correlated (r = 0.9493 for tea and r = 0.8533 for substitute tea).

Conclusions: The proposed method is better than Folin-Ciocalteu method in terms of selectivity, sensitivity and accuracy. It is suitable not only for the determination of polyphenols content in tea, but also for substitute tea.

Highlights: We developed a flow-injection analysis new method for polyphenolic content determination based on Ag(III)-luminol chemiluminescence system. It is simple, rapid, sensitive and accurate. It is suitable not only for the determination of polyphenols content in tea, but also for substitute tea.
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http://dx.doi.org/10.1093/jaoacint/qsac085DOI Listing
July 2022

Long-term outcomes of Spetzler-Martin grade IV and V arteriovenous malformations: a single-center experience.

Neurosurg Focus 2022 Jul;53(1):E12

1Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing.

Objective: This study aimed to explore whether intervention can benefit Spetzler-Martin (SM) grade IV-V arteriovenous malformations (AVMs).

Methods: Eighty-two patients with SM grade IV-V AVMs were retrospectively reviewed from 2015 to 2018. Patients were divided into two groups: those who received conservative management (22 cases [26.8%]) and intervention (60 cases [73.2%], including 21 cases of microsurgery, 19 embolization, and 20 hybrid surgery). Neurofunctional outcomes were assessed with the modified Rankin Scale (mRS). The primary outcome was long-term neurofunctional status, and the secondary outcomes were short-term neurofunctional status, long-term obliteration rate, seizure control, and risk of subsequent hemorrhage.

Results: Regarding the primary outcome, after an average of 4.7 years of clinical follow-up, long-term neurofunctional outcomes were similar after conservative management or intervention (absolute difference -0.4 [95% CI -1.5 to 0.7], OR 0.709 [95% CI 0.461-1.090], p = 0.106), whereas intervention had an advantage over conservative management for avoidance of severe disability (defined as mRS score > 3) (1.7% vs 18.2%, absolute difference 16.5% [95% CI -23.6% to 56.6%], OR 0.076 [95% CI 0.008-0.727], p = 0.025). Regarding the secondary outcomes, intervention was conducive to better seizure control (Engel class I-II) (70.0% vs 0.0%, absolute difference 70.0% [95% CI 8.6%-131.4%], p = 0.010) and avoidance of subsequent hemorrhage (1.4% vs 6.0%, absolute difference 4.6% [95% CI -0.4% to 9.6%], p = 0.030). In the subgroup analysis based on different intervention modalities, microsurgery and hybrid surgery achieved higher complete obliteration rates than embolization (p < 0.001), and hybrid surgery resulted in significantly less intraoperative blood loss than microsurgery (p = 0.041).

Conclusions: Intervention is reasonable for properly indicated SM grade IV-V AVMs because it provides satisfactory seizure control with decreased risks of severe disability and subsequent hemorrhage than conservative management. Clinical trial registration no.: NCT04572568 (ClinicalTrials.gov).
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http://dx.doi.org/10.3171/2022.4.FOCUS21648DOI Listing
July 2022

Updating Urinary Microbiome Analyses to Enhance Biologic Interpretation.

Front Cell Infect Microbiol 2022 8;12:789439. Epub 2022 Jul 8.

Division of Bioinformatics and Computational Biomedicine, Department of Medical Informatics and Clinical Epidemiology, Oregon Health & Science University, Portland, OR, United States.

Objective: An approach for assessing the urinary microbiome is 16S rRNA gene sequencing, where analysis methods are rapidly evolving. This re-analysis of an existing dataset aimed to determine whether updated bioinformatic and statistical techniques affect clinical inferences.

Methods: A prior study compared the urinary microbiome in 123 women with mixed urinary incontinence (MUI) and 84 controls. We obtained unprocessed sequencing data from multiple variable regions, processed operational taxonomic unit (OTU) tables from the original analysis, and de-identified clinical data. We re-processed sequencing data with DADA2 to generate amplicon sequence variant (ASV) tables. Taxa from ASV tables were compared to the original OTU tables; taxa from different variable regions after updated processing were also compared. Bayesian graphical compositional regression (BGCR) was used to test for associations between microbial compositions and clinical phenotypes (e.g., MUI versus control) while adjusting for clinical covariates. Several techniques were used to cluster samples into microbial communities. Multivariable regression was used to test for associations between microbial communities and MUI, again while adjusting for potentially confounding variables.

Results: Of taxa identified through updated bioinformatic processing, only 40% were identified originally, though taxa identified through both methods represented >99% of the sequencing data in terms of relative abundance. Different 16S rRNA gene regions resulted in different recovered taxa. With BGCR analysis, there was a low (33.7%) probability of an association between overall microbial compositions and clinical phenotype. However, when microbial data are clustered into bacterial communities, we confirmed that bacterial communities are associated with MUI. Contrary to the originally published analysis, we did not identify different associations by age group, which may be due to the incorporation of different covariates in statistical models.

Conclusions: Updated bioinformatic processing techniques recover different taxa compared to earlier techniques, though most of these differences exist in low abundance taxa that occupy a small proportion of the overall microbiome. While overall microbial compositions are not associated with MUI, we confirmed associations between certain communities of bacteria and MUI. Incorporation of several covariates that are associated with the urinary microbiome improved inferences when assessing for associations between bacterial communities and MUI in multivariable models.
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http://dx.doi.org/10.3389/fcimb.2022.789439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309214PMC
July 2022

Flexible coding scheme for robotic arm control driven by motor imagery decoding.

J Neural Eng 2022 Jul 27. Epub 2022 Jul 27.

School of Information Engineering, Wuhan University of Technology, No.122,Luoshi Road, Hongshan District, Wuhan, Hubei, 430070, CHINA.

Objective: Brain computer interface (BCI) technology is a new way of information exchange, which can effectively convert physiological signals into control instructions of machines. Due to its spontaneity and device-independence, motor imagery (MI) electroencephalography (EEG) signal is used as a common BCI signal source to achieve direct control of external devices. However, the generalization ability of current classification model of MI tasks is still limited. Moreover, the real time prototype is far from established in practice.

Approach: In order to solve these problems, this paper proposed an optimized neural network architecture based on our previous work. Firstly, the artifact components in MI-EEG signal are removed by using the threshold and threshold function related to the artifact removal evaluation index, and then the data is augmented by the empirical mode decomposition (EMD) algorithm. Furthermore, ensemble learning (EL) method and fine-tuning strategy in transfer learning (TL) are used to optimize the classification model. Finally, combined with the flexible binary encoding strategy, the EEG signal recognition results are mapped to the control commands of the robotic arm, which realizes the multiple degree of freedom control of the robotic arm.

Main Results: The results show that EMD has an obvious data amount enhancement effect on small dataset, and the EL and TL can improve intra-subject and inter-subject model evaluation performance, respectively. The use of binary coding method realizes the expansion of control instructions, i.e., four kinds of MI-EEG signals are used to complete the control of 7 degrees of freedom of the robotic arm.

Significance: Our work not only improves the classification accuracy of subject and the generality of classification model, but also extends the BCI control instruction set.
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http://dx.doi.org/10.1088/1741-2552/ac84a9DOI Listing
July 2022

Beta-Glucan Alters Gut Microbiota and Plasma Metabolites in Pre-Weaning Dairy Calves.

Metabolites 2022 Jul 26;12(8). Epub 2022 Jul 26.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

The present study aims to evaluate the alterations in gut microbiome and plasma metabolites of dairy calves with β-glucan (BG) supplementation. Fourteen healthy newborn dairy calves with similar body weight were randomly divided into control ( = 7) and BG ( = 7) groups. All the calves were fed on the basal diet, while calves in the BG group were supplemented with oat BG on d 8 for 14 days. Serum markers, fecal microbiome, and plasma metabolites at d 21 were analyzed. The calves were weaned on d 60 and weighed. The mean weaning weight of the BG group was 4.29 kg heavier than that of the control group. Compared with the control group, the levels of serum globulin, albumin, and superoxide dismutase were increased in the BG group. Oat BG intake increased the gut microbiota richness and decreased the Firmicutes-to-Bacteroidetes ratio. Changes in serum markers were found to be correlated with the plasma metabolites, including sphingosine, trehalose, and 3-methoxy-4-hydroxyphenylglycol sulfate, and gut microbiota such as , , and . Overall, these results suggest that the BG promotes growth and health of pre-weaning dairy calves by affecting the interaction between the host and gut microbiota.
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http://dx.doi.org/10.3390/metabo12080687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332571PMC
July 2022

Soil Chemical Properties, Metabolome, and Metabarcoding Give the New Insights into the Soil Transforming Process of Fairy Ring Fungi .

J Fungi (Basel) 2022 Jun 28;8(7). Epub 2022 Jun 28.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, 100 Daxue Rd., Nanning 530004, China.

A unique ecological landscape distributed in the Mongolian Plateau, called fairy rings, caused by the growth of the fungus (LM) in the soil could promote plant growth without fertilization. Therefore, this landscape can alleviate fertilizer use and has excellent value for agricultural production. The previous studies only investigated several parameters of the fairy rings, such as soil microbial diversity and some soil chemical properties, thus conclusions based on the studies on fairy rings lack comprehension. Therefore, the present study systematically investigated the chemical properties, metabolome, and metabarcoding of LM-transformed soil. We analyzed fairy ring soils from DARK (FR) and OUT (CK) zone correlated growth promotion with ten soil chemical properties, including N, nitrate-N, inorganic-P, cellulose, available boron, available sulfur, Fe, Mn, Zn, and Cu, which were identified as important markers to screen fairy ring landscapes. Metabolomics showed that the accumulation of 17 carbohydrate-dominated metabolites was closely associated with plant growth promotion. Finally, metabarcoding detected fungi as the main components affecting soil conversion. Among the various fungi at the family level, Lasiosphaeriaceae, unidentified_Auriculariales_sp, and Herpotrichiellaceae were markers to screen fairy ring. Our study is novel and systematically reveals the fairy ring soil ecology and lists the key factors promoting plant growth. These findings lay a theoretical foundation for developing the fairy ring landscape in an agricultural system.
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http://dx.doi.org/10.3390/jof8070680DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324422PMC
June 2022

The Impacts of Climate Change on the Potential Distribution of (Hübner) (Lepidoptera: Pyralidae) in China.

Insects 2022 Jul 15;13(7). Epub 2022 Jul 15.

College of Plant Protection, Shanxi Agricultural University, Jinzhong 030801, China.

The Indian meal moth (Hübner) (Lepidoptera: Pyralidae) is a notorious stored-grain pest that can be found in most parts of China. The corpses, excretions, and other secretions of larvae cause serious grain pollution, seriously affecting the nutritional and economic value of stored grain in China. To elucidate the potential distribution of in China, we used the CLIMEX 4.0 model to project the potential distribution of the pest using historical climate data (1960-1990) and estimated future climate data (2030, 2050, and 2070). Under the historical climate situation, was distributed in most areas of China, and its highly favorable habitats account for 48.14% of its total potential distribution. Because of temperature change in the future climate, suitable habitats will increase in the eastern part of Qinghai and will decrease in the mid-eastern, northeastern, and southeastern parts of China. Under these scenarios, the area of this pest's highly favorable habitat will be reduced by 1.24 million km, and its proportion will decrease to about 28.48%. These predicted outcomes will help to distinguish the impact of climate change on the potential distribution of , thereby providing important information to design early forecasting and strategies to prevent pest harm to stored grain.
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http://dx.doi.org/10.3390/insects13070636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318193PMC
July 2022

Polarization Attack on Continuous-Variable Quantum Key Distribution with a Local Local Oscillator.

Entropy (Basel) 2022 Jul 18;24(7). Epub 2022 Jul 18.

Science and Technology on Communication Security Laboratory, Institute of Southwestern Communication, Chengdu 610041, China.

The estimation of phase noise of continuous-variable quantum key distribution protocol with a local local oscillator (LLO CVQKD), as a major process in quantifying the secret key rate, is closely relevant to the intensity of the phase reference. However, the transmission of the phase reference through the insecure quantum channel is prone to be exploited by the eavesdropper (Eve) to mount attacks. Here, we introduce a polarization attack scheme against the phase reference. Presently, in a practical LLO CVQKD system, only part of the phase reference pulses are measured to compensate for the polarization drift of the quantum signal pulses in a compensation cycle due to the limited polarization measurement rate, while the other part of the phase reference pulses are not measured. We show that Eve can control the phase noise by manipulating the polarization direction of the unmeasured phase reference to hide her attack on the quantum signal. Simulations show that Eve can obtain partial or total key rates information shared between Alice and Bob as the transmission distance increases. Improving the polarization measurement rate to 100% or monitoring the phase reference intensity in real-time is of great importance to protect the LLO CVQKD from polarization attack.
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http://dx.doi.org/10.3390/e24070992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320017PMC
July 2022

Transcriptomic and Widely Targeted Metabolomic Approach Identified Diverse Group of Bioactive Compounds, Antiradical Activities, and Their Associated Genes in Six Sugarcane Varieties.

Antioxidants (Basel) 2022 Jul 4;11(7). Epub 2022 Jul 4.

Guangxi Key Laboratory of Sugarcane Biology, College of Agriculture, Guangxi University, 100 Daxue Rd., Nanning 530004, China.

Sugarcane is cultivated mainly for its high sucrose content but it can also produce many metabolites with promising antioxidant potential. However, very few studies have been reported on the biosynthesis of metabolites in sugarcane to date. In this study, we have identified a wide range of amino acids and organic acids in the rind of six sugarcane varieties by the LC-MS/MS method. A total number of 72 amino acids and 55 organic acid compounds were characterized; among these, 100 were reported for the first time. Moreover, 13 amino acids and seven organic acids were abundantly distributed in all varieties tested and considered major amino acids and organic acids in sugarcane. The variety Taitang134 (F134) showed the highest content of total amino acids, whereas the varieties ROC16 and Yuetang93/159 (YT93/159) had maximum content of organic acids. The amino acids of the rind extract presented higher antioxidant capacity than the organic acids of the rind extract. In addition, the transcriptomic and metabolic integrated analysis highlighted some candidate genes associated with amino acid biosynthesis in sugarcane. We selected a transcription factor gene, (), and over-expressed it in . The transgenic plants showed a higher accumulation of amino acids with higher antiradical activity compared with the wild-type plants. Thus, we characterize a wide range of amino acids and organic acids and their antiradical activities in different sugarcane varieties and present candidate genes that can be potentially valuable for the genetic improvement of metabolites in sugarcane bagasse.
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http://dx.doi.org/10.3390/antiox11071319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311902PMC
July 2022

LIF-ting ferroptosis to improve liver cancer therapy.

Genes Dis 2022 Sep 28;9(5):1153-1155. Epub 2022 Mar 28.

Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

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http://dx.doi.org/10.1016/j.gendis.2022.02.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293704PMC
September 2022

A Network Meta-Analysis of the Dose-Response Effects of Dapagliflozin on Efficacy and Safety in Adults With Type 1 Diabetes.

Front Endocrinol (Lausanne) 2022 7;13:923376. Epub 2022 Jul 7.

Department of Endocrine, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University, Urumqi, China.

Background: Most patients with type 1 diabetes (T1DM) do not reach the blood glucose goal with treatment of insulin. In our research, we intended to estimate the therapeutic effect and safety of additional different doses of dapagliflozin on insulin treatment in T1DM.

Methods: We performed direct and indirect network meta-analysis using Bayesian models and graded different dosages of dapagliflozin by mixed therapy contrasts. We retrieved information from the PubMed, Embase, The Cochrane Library, Web of Science, China Biology Medicine (CBM) disc, China National Knowledge Infrastructure (CNKI), Wanfang Data, and WEIPU Data. Our research included randomized controlled trials (RCTs) including T1DM treated with insulin and additional dapagliflozin 5 mg or dapagliflozin 10 mg from January 2012 to June 2021. Thirteen RCTs with 10,701 participants were divided into three groups as below: insulin alone, dapagliflozin 5 mg + insulin, and dapagliflozin 10 mg + insulin.

Results: Dapagliflozin dose-dependently exhibited reductions in glycated hemoglobin (HbA1c), total insulin daily dose (TDD), and body weight. Neither dapagliflozin 5 mg nor 10 mg could induce hypoglycemia or severe hypoglycemia. However, both doses of dapagliflozin increased the incidence of diabetic ketoacidosis (DKA) and genital infection.

Conclusions: Dapagliflozin 10 mg could achieve a better outcome in efficacy and could not increase the risk of hypoglycemia. Although it may induce a higher risk of DKA and genital infection, there was no significant difference between dapagliflozin 10 mg and 5 mg. Our outcomes indicate that dapagliflozin 10mg has a high reliability of being graded prior as a supplementary treatment to insulin in T1DM.
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http://dx.doi.org/10.3389/fendo.2022.923376DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301373PMC
July 2022

A Phenotype and Genotype Case Report of a Neonate With Congenital Bilateral Coronary Artery Fistulas and Multiple Collateral Arteries.

Front Cardiovasc Med 2022 6;9:939551. Epub 2022 Jul 6.

Clinical Physiology Laboratory, Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangdong, China.

We report a unique case of an 18-day-old girl with three coronary artery fistulas to the right atrium and right ventricle, respectively: three collateral arteries arising from the descending aorta and one from the right subclavian artery draining through a sac to the top of the right atrium, patent ductus arteriosus, and atrial septal defect. She presented symptoms of acute congestive heart failure. Cardiac catheterization and surgical interventions were performed to repair the defects. The patient recovered uneventfully and grew up well at 3 years of follow-up. Whole-genome sequencing (WES) in the patient, compared to her parents, showed 17 variants within 11 genes. Among these, only compound heterozygous mutation, c.T470G (p.L157R) and c.A1622G (p.D541G), in the gene have been reportedly related to congenital heart disease and are the most likely causative in our patient.
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http://dx.doi.org/10.3389/fcvm.2022.939551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9299261PMC
July 2022

The effect of sex differences on complications and 90-day outcomes after aneurysmal subarachnoid hemorrhage: a propensity score-matched analysis.

Neurosurg Rev 2022 Jul 23. Epub 2022 Jul 23.

Department of Neurosurgery, Fengtai District, Beijing Tiantan Hospital, Capital Medical University, No. 119 South Fourth Ring West Road, Beijing, 100070, China.

Aneurysmal subarachnoid hemorrhage (aSAH) is the most devastating form of stroke. Up to now, little is known about the effect of sex differences on complications and outcomes. We retrospectively reviewed aSAH patients admitted to our institution between January 2015 and December 2020. The functional outcomes at discharge and 90 days after discharge were assessed using the modified Rankin Scale (mRS). Baseline characteristics, in-hospital complications, and outcomes were compared after 1:1 propensity score matching (PSM). The area under the curve (AUC) in the receiver operating characteristic curve (ROC) analysis was calculated to measure each independent risk factor's prediction ability. A total of 833 patients were included. After PSM, 109 male patients were compared with 109 female patients. Female patients had a higher incidence of anemia (47/109 [43.1%] vs. 30/109 [27.5%], p = 0.016) than male patients, while male patients had a higher incidence of pneumonia (36/109 [33.0%] vs. 19/109 [17.4%], p = 0.008) than female patients. No significant differences were found in the rate of unfavorable outcomes at discharge and 90-day outcomes (40/109 [36.7%] vs. 50/109 [45.9%], p = 0.169; 15/109 [13.8%] vs. 19/107 [17.8%], p = 0.420) between female and male patients. Pneumonia (AUC = 0.749, 95% confidence interval [CI] = 0.623-0.875, p < 0.001) and anemia (AUC = 0.753, 95% CI = 0.632-0.873, p = 0.002) showed good ability to predict 90-day unfavorable outcomes in male and female patients, respectively. Female patients had a higher incidence of anemia but a lower incidence of pneumonia during hospitalization. However, differences in in-hospital complications did not result in differences in outcomes between women and men. Clinical Trial Registration: NCT04785976. 2021/03/05, retrospectively registered.
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http://dx.doi.org/10.1007/s10143-022-01836-xDOI Listing
July 2022

Investigation of rumen long noncoding RNA before and after weaning in cattle.

BMC Genomics 2022 Jul 22;23(1):531. Epub 2022 Jul 22.

Department of Animal and Avian Sciences, University of Maryland, 8127 Regents Drive, MD, 20742, College Park, USA.

Background: This study aimed to identify long non-coding RNA (lncRNA) from the rumen tissue in dairy cattle, explore their features including expression and conservation levels, and reveal potential links between lncRNA and complex traits that may indicate important functional impacts of rumen lncRNA during the transition to the weaning period.

Results: A total of six cattle rumen samples were taken with three replicates from before and after weaning periods, respectively. Total RNAs were extracted and sequenced with lncRNA discovered based on size, coding potential, sequence homology, and known protein domains. As a result, 404 and 234 rumen lncRNAs were identified before and after weaning, respectively. However, only nine of them were shared under two conditions, with 395 lncRNAs found only in pre-weaning tissues and 225 only in post-weaning samples. Interestingly, none of the nine common lncRNAs were differentially expressed between the two weaning conditions. LncRNA averaged shorter length, lower expression, and lower conservation scores than the genome overall, which is consistent with general lncRNA characteristics. By integrating rumen lncRNA before and after weaning with large-scale GWAS results in cattle, we reported significant enrichment of both pre- and after-weaning lncRNA with traits of economic importance including production, reproduction, health, and body conformation phenotypes.

Conclusions: The majority of rumen lncRNAs are uniquely expressed in one of the two weaning conditions, indicating a functional role of lncRNA in rumen development and transition of weaning. Notably, both pre- and post-weaning lncRNA showed significant enrichment with a variety of complex traits in dairy cattle, suggesting the importance of rumen lncRNA for cattle performance in the adult stage. These relationships should be further investigated to better understand the specific roles lncRNAs are playing in rumen development and cow performance.
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http://dx.doi.org/10.1186/s12864-022-08758-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308236PMC
July 2022

The role of hippocampal KCNQ2 channel in antidepressant actions of ketamine.

Neuron 2022 07;110(14):2201-2203

Division of Clinical Neuroscience, Chiba University Center for Forensic Mental Health, Chiba 260-8670, Japan. Electronic address:

In this issue of Neuron, Lopez et al. report that KCNQ2 (potassium voltage-gated channel subfamily Q member 2) is essential for the sustained antidepressant-like effects of ketamine in glutamatergic neurons of the ventral hippocampus. This study implies that KCNQ2 activators can be novel antidepressants without the ketamine side effects.
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http://dx.doi.org/10.1016/j.neuron.2022.05.027DOI Listing
July 2022

A Two-Stream Graph Convolutional Network Based on Brain Connectivity for Anesthetized States Analysis.

IEEE Trans Neural Syst Rehabil Eng 2022 28;30:2077-2087. Epub 2022 Jul 28.

Investigating neural mechanisms of anesthesia process and developing efficient anesthetized state detection methods are especially on high demand for clinical consciousness monitoring. Traditional anesthesia monitoring methods are not involved with the topological changes between electrodes covering the prefrontal-parietal cortices, by investigating electrocorticography (ECoG). To fill this gap, a framework based on the two-stream graph convolutional network (GCN) was proposed, i.e., one stream for extracting topological structure features, and the other one for extracting node features. The two-stream graph convolutional network includes GCN Model 1 and GCN Model 2. For GCN Model 1, brain connectivity networks were constructed by using phase lag index (PLI), representing different structure features. A common adjacency matrix was founded through the dual-graph method, the structure features were expressed on nodes. Therefore, the traditional spectral graph convolutional network can be directly applied on the graphs with changing topological structures. On the other hand, the average of the absolute signal amplitudes was calculated as node features, then a fully connected matrix was constructed as the adjacency matrix of these node features, as the input of GCN Model 2. This method learns features of both topological structure and nodes of the graph, and uses a dual-graph approach to enhance the focus on topological structure features. Based on the ECoG signals of monkeys, results show that this method which can distinguish awake state, moderate sedation and deep sedation achieved an accuracy of 92.75% in group-level experiments and mean accuracy of 93.50% in subject-level experiments. Our work verifies the excellence of the graph convolutional network in anesthesia monitoring, the high recognition accuracy also shows that the brain network may carry neurological markers associated with anesthesia.
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http://dx.doi.org/10.1109/TNSRE.2022.3193103DOI Listing
August 2022
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