Publications by authors named "Li Lin"

6,271 Publications

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Identification of suspended particulate matters as the hotspot of polycyclic aromatic hydrocarbon degradation-related bacteria and genes in the Pearl River Estuary using metagenomic approaches.

Chemosphere 2021 Jul 23;286(Pt 1):131668. Epub 2021 Jul 23.

Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Marine Sciences, Sun Yat-sen University, Zhuhai, 510275, China. Electronic address:

Bacterial degradation is unequivocally considered as an important way for the cleanup of polycyclic aromatic hydrocarbon (PAHs) in the aquatic environment. However, the diversity and distribution of PAH-degrading bacterial communities and PAH degradation-related genes (PAHDGs) in ambient environment need to be investigated. In this study, bacteria in the water of the Pearl River Estuary (PRE) were initially separated as the particle-attached bacteria (PAB) and free-living bacteria (FLB), and were further characterized using metagenomic approaches. Proteobacteria (80.1 %) was identified as the most abundant PAH-degrading phylum in the PRE water, followed by Bacteroidetes, Actinobacteria, and Firmicutes. A substantial difference in the community structure was observed between PAH-degrading PAB and FLB. Both of PAH-degrading bacteria and PAHDGs were enriched on the suspended particulate matters (SPMs), with the range of enrichment factor (EF) from 7.84 × 10 to 6.64 × 10 (PAH-degrading bacteria) and from 1.14 × 10 to 1.76 × 10 (PAHDGs). The levels of PAH-degrading bacteria 16 S rRNA genes and PAHDGs on the SPMs were both significantly correlated with those in the aqueous phase (AP) in the PRE water (p < 0.05), indicating a dynamic distribution of PAH-degrading bacteria between these two phases. The total PAH concentrations on the SPMs of the PRE water were also significantly correlated with the total PAHDG levels in the PAB (p < 0.05). Our results suggested that the SPMs could be the important compartment for the elimination of PAHs from the aquatic environment.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131668DOI Listing
July 2021

Post-stroke treatment of storax improves long-term outcomes of stroke in rats.

J Ethnopharmacol 2021 Jul 31:114467. Epub 2021 Jul 31.

Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China; School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China. Electronic address:

Ethnopharmacological Relevance: The lack of widely applicable pharmacological treatments for ischemic stroke patients has led to a growing interest in traditional medicine. The identification of pharmacologically active components of the clinically used traditional medicine has been considered translationally significant. SuHeXiang Wan is a prescription containing 15 herbs approved by Chinese National Drug Administration (CNDA) for treating ischemic stroke. Storax is one of the main contents in this prescription and is believed to play a significant pharmacological role, which has been used to treat stroke for nearly 1000 years in traditional Chinese medicine. Emerging experimental investigations suggested Storax might be effective for treating ischemic stroke.

Aim Of The Study: This study aimed to test our hypothesis that post-stroke treatment of Storax can improve long-term outcomes of stroke.

Materials And Methods: Male Wistar rats (250-280 g) subjected to 2 h of MCAO following reperfusion were treated with Storax by intragastric at 1 h and repeated at 3 h, 6 h after stroke. In the first set experiment, an array of neurological function deficits assessments were tested before and after stroke, brain lesion size was examined at 28 days after ischemia. CD31 and synatophysin were analyzed by immunohistochemistry. In the second set experiment, markers of proinflammatory activation were determined at 24 h after stroke. ELISA was performed to analyze brain concentrations of TNF-α, IL-1β and circulating levels of iNOS, ET-1, and immunohistochemistry was performed to determine GFAP, IBA-1 and NF-κB p65.

Results: Storax significantly alleviated neurological deficits from 7 days after stroke and lasted until 28 days, corresponding to the significantly decreased lesion volume at 28 days after stroke; Meanwhile, Storax increased the density of CD31and SYP in peri-infarct areas. At 24 h after stroke, Storax significantly inhibited brain TNF-α, IL-1β expression and circulating iNOS, ET-1 levels, reduced the NF-κB/p65 positive cell number, and decreased activated microglia/macrophages and astrocytes cell numbers alongside reversed their morphological transformations.

Conclusion: Our experimental findings demonstrate treatment of Storax at the acute phase significantly improves long-term neurological outcomes in the focal stroke model of rats. We also speculate that inhibition of acute proinflammation activation by Storax might be associated with its beneficial pharmacological effect, but remain to define and elucidate in future investigation.
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http://dx.doi.org/10.1016/j.jep.2021.114467DOI Listing
July 2021

Lignosulfonate/diblock copolymer polyion complexes with aggregation-enhanced and pH-switchable fluorescence for information storage and encryption.

Int J Biol Macromol 2021 Jul 31. Epub 2021 Jul 31.

State Key Laboratory of Heavy Oil Processing, College of Chemical Engineering, China University of Petroleum, Qingdao, Shandong 266580, China. Electronic address:

Employing natural polymers as building blocks is favorable to construct sustainable functional materials and realize biomass utilization. Here we developed a series of supramolecular polyion complexes (PICs) composed of lignosulfonate and double-hydrophilic diblock copolymer poly(ethylene oxide-b-N, N-dimethylaminoethyl methacrylate) (PEO-b-PDMAEMA), which performed a blue emission with quenching-enhancing tendency and copolymer distribution inversion-involved assembling transformation at pH 5.6 and monotonic greenish-blue fluorescence promotion at pH 9.5 by increasing PEO-b-PDMAEMA contents. Electrostatic interactions and multiple hydrogen bonds were revealed controlled the assembling behavior and affected the emission via altering the restriction of molecular motion, through-space conjunction, and non-luminous complexation. The multiple interacting sites and special topology of diblock copolymer contributed to the efficient fluorescence regulation. Information writing-erasing and encryption-decryption systems were established by utilizing emission intensity regulation and pH-responsive emission chromism. This work paved a new way to enhance lignin fluorescence and broadened potential applications of lignin composites in realms of sensing, imaging, monitoring, and anticounterfeiting.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.173DOI Listing
July 2021

Improvement of Skin Barrier Dysfunction by Phenolic-containing Extracts of Lycium Barbarum via Nrf2/HO-1 Regulation.

Photochem Photobiol 2021 Aug 3. Epub 2021 Aug 3.

The School of Biomedical and Pharmaceutical Engineering, Guangdong University of Technology, Guangzhou, P. R. China.

Lycium barbarum have received an increasing popularity due to its powerful biological activity and medicinal use. However, the effect of Lycium barbarum on skin remain largely uncharacterized. The general purpose of this paper was to characterize the phenolic compounds in Lycium barbarum extract (LBE) using LC-HRMS/QTOF method and to investigate whether topical administration of LBE can repair skin barrier dysfunction in mice. Our data demonstrated that LBE could not only decrease ROS level and matrix metalloproteinase expression, but also strengthen intrinsic antioxidant defense system including SOD, GSH-Px and CAT, thereby resulting in increased skin collagen content and an improvement of UV-induced skin erythema, thickness and wrinkles. Improved skin barrier functions were highly correlated with increased expression of filaggrin, involucrin and loricrin as well as antioxidant proteins such as Nrf2 and HO-1 in UV-irradiated mice, suggesting that LBE may be promising natural products at a lower cost for the topical application in the treatment of skin diseases with defective barrier function.
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http://dx.doi.org/10.1111/php.13498DOI Listing
August 2021

Iterative channel estimation-based soft successive interference cancellation for multiuser underwater acoustic communications.

J Acoust Soc Am 2021 Jul;150(1):133

Acoustic Science and Technology Laboratory, Harbin Engineering University, Harbin 150001, China.

This paper presents a single-carrier multiuser receiver in underwater acoustic communications with strong multiple access interference (MAI) combining passive time-reversal (PTR) and direct-adaptation-based turbo equalization. The receiver works in an iterative block by block manner. In the first iteration, when the symbols of all users are detected by successive interference cancellation (SIC) in the block, the channel impulse response (CIR) of each user is then updated by post-SIC signal to achieve more accurate MAI reconstruction and PTR combination for the next block. In the following iterations, the a priori mean of symbol in the current block is available and used to further improve the accuracy of reconstructed MAI and the updated CIR. The proposed receiver is demonstrated using experimental data collected at Songhua Lake, China, in 2019. The results show that single-carrier nine-user communication data with quadrature phase-shift keying can be successfully recovered, with an average data rate of 1.67 kbps for each user.
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http://dx.doi.org/10.1121/10.0005476DOI Listing
July 2021

Porous gelatin microspheres for controlled drug delivery with high hemostatic efficacy.

Colloids Surf B Biointerfaces 2021 Jul 28;207:112013. Epub 2021 Jul 28.

Orthopaedic Oncology Center, Department of Orthopedics, Changzheng Hospital, Second Military Medical University, Shanghai, China. Electronic address:

Effective hemostasis and antibacterial efficacy for extensive trauma in a warzone and civilian accidents are important for reducing mortalities and serious complications. Gelatin has been widely used as a hemostatic agent and has the potential for use in drug delivery systems. To enhance its hemostatic efficiency and create conducive conditions for sustained drug release, we developed Vancomycin-impregnated porous gelatin microspheres (Van-MS) by introducing the porous structure into gelatin. Results showed that Van-MS can be successfully developed via the ice crystal pore-making method combined with hydration maintaining its stability. We also explored the use of biodegradable porous materials for treatment of infections and bleeding in soft tissue, and analyzed Van-MS via scanning electron microscopy (SEM), scanning electron microscopy and energy dispersive X-ray spectrometry (SEM-EDS), Fourier Transform infrared spectroscopy (FTIR) and High-Performance Liquid Chromatography (HPLC). Results from Van-MS showed high hemostatic both efficacies in vivo and in vitro. Moreover, muscle lesions treated by Van-MS showed formation of fibrous connective tissue and were nearly sealed after 10 days in a rabbit traumatic infection model. This antibacterial performance was attributed to absorption of exudates and sustained drug release. Hemostatic effects were due to: (1) particles water swelling form a physical barrier that led to physical hemostasis; (2) activation of the endogenous coagulation pathway which resulted in physiological hemostasis; (3) aggregation of platelets and erythrocytes after absorbing water; and (4) stronger hemostatic properties owing to their porous structure with high specific surface area.
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http://dx.doi.org/10.1016/j.colsurfb.2021.112013DOI Listing
July 2021

Reducing cadmium in rice using metallothionein surface-engineered bacteria WH16-1-MT.

Environ Res 2021 Jul 30:111801. Epub 2021 Jul 30.

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China. Electronic address:

Cadmium (Cd) accumulation in rice grains poses a health risk for humans. In this study, a bacterium, Alishewanella sp. WH16-1-MT, was engineered to express metallothionein on the cell surface. Compared with the parental WH16-1 strain, Cd adsorption efficiency of WH16-1-MT in medium was increased from 1.2 to 2.6 mg/kg dry weight. The WH16-1-MT strain was then incubated with rice in moderately Cd-contaminated paddy soil. Compared with WH16-1, inoculation with WH16-1-MT increased plant height, panicle length and thousand-kernel weight, and decreased the levels of ascorbic acid and glutathione and the activity of peroxidase. Compared with WH16-1, WH16-1-MT inoculation significantly reduced the concentrations of Cd in brown rice, husks, roots and shoots by 44.0 %, 45.5 %, 36.1 % and 47.2 %, respectively. Moreover, inoculation with WH16-1-MT reduced the bioavailability of Cd in soil, with the total Cd proportion in oxidizable and residual states increased from 29 % to 32 %. Microbiome analysis demonstrated that the addition of WH16-1-MT did not significantly alter the original bacterial abundance and community structure in soil. These results indicate that WH16-1-MT can be used as a novel microbial treatment approach to reduce Cd in rice grown in moderately Cd-contaminated paddy soil.
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http://dx.doi.org/10.1016/j.envres.2021.111801DOI Listing
July 2021

Purinergic signaling: a potential therapeutic target for depression and chronic pain.

Purinergic Signal 2021 Aug 2. Epub 2021 Aug 2.

Neuropharmacology Laboratory of Physiology Department, Basic Medical School, Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.

The comorbid mechanism of depression and chronic pain has been a research hotspot in recent years. Until now, the role of purinergic signals in the comorbid mechanism of depression and chronic pain has not been fully understood. This review mainly summarizes the research results published in PubMed during the past 5 years and concludes that purinergic signaling is a potential therapeutic target for comorbid depression and chronic pain, and the purinergic receptors A1, A2A, P2X3, P2X4, and P2X7and P2Y, P2Y, and P2Y may be important factors. The main potential pathways are as follows: A1 receptor-related G protein-dependent activation of introverted K channels (GIRKs), A2A receptor-related effects on the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) and MAPK/nuclear factor-κB (NF-κB) pathways, P2X3 receptor-related effects on dorsal root ganglia (DRG) excitability, P2X4 receptor-related effects on proinflammatory cytokines and inflammasome activation, P2X7 receptor-related effects on ion channels, the NLRP3 inflammasome and brain-derived neurotrophic factor (BDNF), and P2Y receptor-related effects on the phospholipase C (PLC)/inositol triphosphate (IP3)/Ca signaling pathway. We hope that the conclusions of this review will provide key ideas for future research on the role of purinergic signaling in the comorbid mechanism of depression and chronic pain.
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http://dx.doi.org/10.1007/s11302-021-09801-xDOI Listing
August 2021

Perampanel Reduces Hyperthermia-Induced Seizures in Dravet Syndrome Mouse Model.

Front Pharmacol 2021 14;12:682767. Epub 2021 Jul 14.

Department of Neurology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.

Treatment options for Dravet syndrome are limited. The aim of this study was to evaluate the antiepileptic effect of the AMPA receptor antagonist perampanel (PER) on a mouse model of Dravet syndrome ( ). We report here that the PER (2 mg/kg) treatment inhibited the spontaneous recurrent seizures and attenuated epileptic activity in mice. In the hyperthermia-induced seizure experiment, PER clearly increased temperature tolerance and significantly ameliorated seizure frequency and discharge duration. PER also demonstrated antiepileptic effects in a cross-over study and a synergistic effect for attenuating heat-induced seizure when given in combination with stiripentol or valproic acid. The results showed that PER effectively decreased the occurrence of spontaneous recurrent seizures and showed significant therapeutic potential for hyperthermia-induced seizures with regard to both susceptibility and severity in a Dravet syndrome mouse model. Potential therapeutic effects of PER for treatment of Dravet syndrome were demonstrated.
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http://dx.doi.org/10.3389/fphar.2021.682767DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317459PMC
July 2021

Tetrahydroxy stilbene glycoside ameliorates Alzheimer's disease in APP/PS1 mice via glutathione peroxidase related ferroptosis.

Int Immunopharmacol 2021 Jul 29;99:108002. Epub 2021 Jul 29.

Department of Pharmacy, Xuanwu Hospital of Capital Medical University, National Clinical Research Center for Geriatric Diseases, Beijing Engineering Research Center for Nervous System Drugs, Beijing Institute for Brain Disorders, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China. Electronic address:

Amyloid beta peptide (Aβ) has been confirmed to be an essential reason of Alzheimer's disease (AD) for a long time. Ferroptosis is a newly recognized oxidative cell death mechanism, which is highly related to AD. Recently, tetrahydroxy stilbene glycoside (TSG) has been beneficial in alleviating learning and memory of AD and aged mouse model. Unfortunately, the underlying mechanisms between TSG and ferroptosis in AD are poorly understood. Herein, we investigated whether neural cells in cerebral cortex and hippocampus that were seriously afflicted in APP/PS1 mice might be vulnerable to ferroptosis. Treatment with non-toxic TSG dose-dependently resisted Aβ-caused cytotoxic death in neuronal cells by regulating ferroptosis related proteins and enzymes in APP/PS1 mice. TSG also alleviated cellular oxidative stress and inflammatory damage in response to Aβ by attenuating the levels of oxidation products. Importantly, TSG administration abrogated Aβ-caused brain damage, indicating that TSG rescued brain cells. Subsequently, TSG promoted the activation of GSH/GPX4/ROS and Keap1/Nrf2/ARE signaling pathways. Notably, markers related to ferroptosis including increased lipid peroxidation, enhanced neuroinflammation such as NLRP3, and also the expression of DMT1, ACSL4 and NCOA4, were reduced by TSG administration. In addition, TSG enhanced antioxidative stress via the upregulation of SOD, and the expression of FTH1, CD98 and xCT. Taken together, our data indicated a novel mechanism of TSG in reversing Aβ-caused injury through restoring mitochondrial function via several signaling pathways, implying a promising candidate against neurodegenerative diseases especially AD. Hence, TSG should be taken into consideration during treatment of AD in the future.
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http://dx.doi.org/10.1016/j.intimp.2021.108002DOI Listing
July 2021

A novel humanized model of rheumatoid arthritis associated lung disease.

Clin Immunol 2021 Jul 29;230:108813. Epub 2021 Jul 29.

Department of Immunology, Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, United States of America; Division of Rheumatology, Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, United States of America. Electronic address:

Cigarette smoking has been implicated in the pathogenesis of seropositive rheumatoid arthritis (RA), as well as RA-associated lung disease. Fibrotic interstitial lung disease as well as emphysema occur in RA and cause substantial morbidity. We used arthritis-susceptible HLA-DQ8 transgenic mice to generate RA-associated lung disease. Mice were exposed to cigarette smoke (CS) prior to induction of arthritis, and subsequently injected with a low dose of bleomycin intra-tracheally to induce lung injury. Exposure of arthritic mice to both CS and bleomycin led to a significant reduction in lung compliance consistent with development of diffuse lung disease. Morphologic evaluation of the lung demonstrated areas of emphysematous change and co-existent fibrosis, consistent with a combined pattern of fibrosis and emphysema. These changes were accompanied by inflammatory cell infiltration and upregulation of fibrosis-associated genes. This humanized mouse model can serve as a valuable research tool to understand the pathogenesis of RA associated lung disease.
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http://dx.doi.org/10.1016/j.clim.2021.108813DOI Listing
July 2021

Subtyping of head and neck squamous cell cancers based on immune signatures.

Int Immunopharmacol 2021 Jul 28;99:108007. Epub 2021 Jul 28.

Biomedical Informatics Research Lab, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China; Cancer Genomics Research Center, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China; Big Data Research Institute, China Pharmaceutical University, Nanjing 211198, China. Electronic address:

Although head and neck squamous cell cancer (HNSCC) is one of the cancer types in which immune checkpoint inhibitors (ICIs) has achieved a certain success, only a subset of HNSCC patients respond to ICIs. Thus, identification of HNSCC subtypes responsive to ICIs is crucial. Using hierarchical clustering, we identified three subtypes of HNSCC, termed Immunity-H, Immunity-M, and Immunity-L, based on the enrichment scores of 28 immune cells generated by the single-sample gene-set enrichment analysis of transcriptome data. We demonstrated that this subtyping method was stable and producible in four different HNSCC cohorts. Immunity-H had the highest levels of immune infiltrates and PD-L1 expression, lowest levels of stemness, intratumor heterogeneity and genomic instability, and favorable prognosis. In contrast, Immunity-L had the lowest levels of immune infiltrates and PD-L1 expression, highest levels of stemness, intratumor heterogeneity and genomic instability, and unfavorable prognosis. We found that somatic copy number alteration had a significant negative association with anti-tumor immunity in HNSCC, while tumor mutation burden showed no significant association. TP53, COL11A1, NSD1, and PKHD1L1 were more frequently mutated in Immunity-H versus Immunity-L, and their mutations were associated with increased immune signatures in HNSCC. Besides immune-related pathways, many stromal and oncogenic pathways were highly enriched in Immunity-H, including cell adhesion molecules, focal adhesion, ECM-receptor interaction, calcium signaling, MAPK signaling, apoptosis, VEGF signaling, and PPAR signaling. The high levels of PD-L1 expression and immune infiltration in Immunity-H indicate that this subtype responds best to ICIs. Our study recaptures the immunological heterogeneity in HNSCC and provide clinical implications for the immunotherapy of HNSCC.
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http://dx.doi.org/10.1016/j.intimp.2021.108007DOI Listing
July 2021

Transport stress affects the fecal microbiota in healthy donkeys.

J Vet Intern Med 2021 Jul 31. Epub 2021 Jul 31.

National Engineering Research Center for Gelatin-based TCM, Dong-E E-Jiao Co., Ltd, Liaocheng, Shandong Province, China.

Background: With the development of large-scale donkey farming in China, long-distance transportation has become common practice, and the incidence of intestinal diseases after transportation has increased. The intestinal microbiota is important in health and disease, and whether or not transportation disturbs the intestinal microbiota in donkeys has not been investigated.

Objectives: To determine the effects of transportation on the fecal microbiota of healthy donkeys using 16S rRNA sequencing.

Animals: Fecal and blood samples were collected from 12 Dezhou donkeys before and after transportation.

Methods: Prospective controlled study. Cortisol, ACTH, and heat-shock protein 90 (HSP90) concentrations were measured. Sequencing of 16S rRNA was used to assess the microbial composition. Alpha diversity and beta diversity were assessed.

Results: Results showed significant (P < .05) increases in cortisol (58.1 ± 14.6 to 71.1 ± 9.60 ng/mL), ACTH (163.8 ± 31.9 to 315.8 ± 27.9 pg/mL), and HSP90 (10.8 ± 1.67 to 14.6 ± 1.75 ng/mL) on the day of arrival. A significantly lower (P = .04) level of bacterial richness was found in fecal samples after transportation, compared with that before transportation without distinct changes in diversity. Most notably, donkeys had significant decreases in Atopostipes, Eubacterium, Streptococcus, and Coriobacteriaceae.

Conclusions And Clinical Importance: Transportation can induce stress in healthy donkeys and have some effect on the composition of the in fecal microbiota. Additional studies are required to understand the potential effect of these microbiota changes, especially significantly decreased bacteria, on the development intestinal diseases in donkeys during recovery from transportation.
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http://dx.doi.org/10.1111/jvim.16235DOI Listing
July 2021

Study on the alleviation of Fengshi Gutong capsule on rheumatoid arthritis through integrating network pharmacology and experimental exploration.

J Ethnopharmacol 2021 Jul 27:114471. Epub 2021 Jul 27.

The MOE Key Laboratory for Standardization of Chinese Medicines, Shanghai Key Laboratory of Compound Chinese Medicines and The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. Electronic address:

Ethnopharmacological Relevance: Fengshi Gutong (FSGT) capsule, a traditional Chinese medicine formula, has effects including warming meridians and dispersing cold, and relieving pain by dredging collaterals. FSGT is generally used for the treatment of rheumatoid arthritis (RA) in clinic in China.

Aim Of The Study: This study aims to investigate the alleviation provided by FSGT capsule on RA in vivo and the engaged mechanism.

Materials And Methods: The collagen-induced arthritis (CIA) mouse model was used to evaluate the alleviation of FSGT capsule on RA in vivo. Network pharmacology was used to find the potential involved molecular targets. Western-blot, Real-time polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were conducted. Wound healing assay was performed in human umbilical vein endothelial cells (HUVECs).

Results: FSGT capsule (300, 900 mg/kg) alleviated RA in CIA mice with no obvious side effects. The results from network pharmacology showed that the top 6 molecular targets involved in the FSGT-provided alleviation on RA were interleukin 6 (IL-6), tumor necrosis factor α (TNFα), C-C motif chemokine 2 (CCL2), vascular endothelial growth factor (VEGF), intercellular cell adhesion molecule-1 (ICAM-1), interleukin 1β (IL-1β), and these results imply the critical participation of inhibiting inflammation or angiogenesis. Next, FSGT capsule decreased the elevated serum contents of rheumatoid factor (RF) and VEGF, and some pro-inflammatory cytokines like TNFα and IL-6. Moreover, FSGT capsule also reduced the elevated protein expression of ICAM1, IL-1β and phosphorylated protein kinase B (Akt) in synovium from CIA mice. Further in vitro results showed that totally 13 compounds from FSGT reduced the enhanced IL-1β and inducible nitric oxide synthase (iNOS) mRNA expression in RAW264.7 macrophages stimulated by lipopolysaccharide (LPS). Meanwhile, 7 compounds from FSGT decreased the VEGF-induced HUVEC migration. Among those compounds, benzoylhypaconine (BHA), pseudoephedrine hydrochloride (PSE), glycyrrhetnic acid (GA), isoliquiritigenin (ISL), quercetin (QUER) and kaempferol (KAE) were found to inhibit both inflammation and angiogenesis in vitro.

Conclusion: FSGT capsule ameliorates RA in CIA mice by reducing inflammation, abrogating angiogenesis and relieving pain. Some compounds in FSGT, including BHA, GA, PSE, ISL, QUER and KAE, reduced both inflammation and angiogenesis in vitro, which suggests that those compounds may contribute to the FSGT capsule-provided alleviation on RA.
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http://dx.doi.org/10.1016/j.jep.2021.114471DOI Listing
July 2021

Self-adaptive metasurface platform based on computer vision.

Opt Lett 2021 Aug;46(15):3520-3523

Programmable metasurfaces allow real-time electromagnetic (EM) manipulation in a digital manner, showing great potential to construct advanced multifunctional EM devices. However, the current programmable metasurfaces typically need human participation to achieve various EM functions. In this Letter, we propose, design, and construct a self-adaptive metasurface platform that can achieve beam control automatically based on image recognition. Such a platform is composed of a metasurface with 36×36 active units, a single-board computer, and two independent cameras that can detect the position of the objects. The single-board computer, Raspberry Pi, is used to calculate the information of the objects and generate the coding sequences to control the digital metasurface based on a low complexity binocular localization algorithm. Such a smart metasurface platform can generate different beams according to the location of the receiver and can be used as intelligent antennas in future communications and radars.
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http://dx.doi.org/10.1364/OL.427527DOI Listing
August 2021

Carbon dots: synthesis, properties and biomedical applications.

J Mater Chem B 2021 Jul 30. Epub 2021 Jul 30.

Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute and School of Basic Medical Science, Central South University, Changsha 410008, China.

Carbon dots (CDs) are a new type of carbon nanomaterial that have unique physical and chemical properties, good biocompatibility, low toxicity, and easy surface functionalization, making them widely used in biological imaging, environmental monitoring, chemical analysis, targeted drug delivery, disease diagnosis, therapy, etc. In this review, our content is mainly divided into four parts. In the first part, we focused on the preparation methods of CDs, including arc discharge, laser ablation, electrochemical oxidation, chemical oxidation, combustion, hydrothermal/solvent thermal, microwave, template, method etc. Next, we summarized methods of CD modification, including heteroatom doping and surface functionalization. Then, we discussed the optical properties of CDs (ultraviolet absorption, photoluminescence, up-conversion fluorescence, etc.). Lastly, we reviewed the common applications of CDs in biomedicine from the aspects of in vivo and in vitro imaging, sensors, drug delivery, cancer theranostics, etc. Furthermore, we also discussed the existing problems and the future development direction of CDs.
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http://dx.doi.org/10.1039/d1tb01077hDOI Listing
July 2021

Large-scale translatome profiling annotates the functional genome and reveals the key role of genic 3' untranslated regions in translatomic variation in plants.

Plant Commun 2021 Jul 23;2(4):100181. Epub 2021 Mar 23.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.

The translatome, a profile of the translational status of genetic information within cells, provides a new perspective on gene expression. Although many plant genomes have been sequenced, comprehensive translatomic annotations are not available for plants due to a lack of efficient translatome profiling techniques. Here, we developed a new technique termed 3' ribosome-profiling sequencing (3'Ribo-seq) for reliable, robust translatomic profiling. 3'Ribo-seq combines polysome profiling and 3' selection with a barcoding and pooling strategy. Systematic translatome profiling of different tissues of , rice, and maize using conventional ribosome profiling (Ribo-seq) and 3'Ribo-seq revealed many novel translational genomic loci, thereby complementing functional genome annotation in plants. Using the low-cost, efficient 3'Ribo-seq technique and genome-wide association mapping of translatome expression (eGWAS), we performed a population-level dissection of the translatomes of 159 diverse maize inbred lines and identified 1,777 translational expression quantitative trait loci (eQTLs). Notably, local eQTLs are significantly enriched in the 3' untranslated regions of genes. Detailed eQTL analysis suggested that sequence variation around the polyadenylation (polyA) signal motif plays a key role in translatomic variation. Our study provides a comprehensive translatome annotation of plant functional genomes and introduces 3'Ribo-seq, which paves the way for deep translatomic analysis at the population level.
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http://dx.doi.org/10.1016/j.xplc.2021.100181DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299070PMC
July 2021

Progress in the Application of Drugs for the Treatment of Multiple Sclerosis.

Front Pharmacol 2021 13;12:724718. Epub 2021 Jul 13.

Department of Pharmacy, Xuanwu Hospital of Capital Medical University, Beijing, China.

Multiple sclerosis (MS) is an autoimmune and chronic inflammatory demyelinating disease of the central nervous system (CNS), which gives rise to focal lesion in CNS and cause physical disorders. Although environmental factors and susceptibility genes are reported to play a role in the pathogenesis of MS, its etiology still remains unclear. At present, there is no complete cure, but there are drugs that decelerate the progression of MS. Traditional therapies are disease-modifying drugs that control disease severity. MS drugs that are currently marketed mainly aim at the immune system; however, increasing attention is being paid to the development of new treatment strategies targeting the CNS. Further, the number of neuroprotective drugs is presently undergoing clinical trials and may prove useful for the improvement of neuronal function and survival. In this review, we have summarized the recent application of drugs used in MS treatment, mainly introducing new drugs with immunomodulatory, neuroprotective, or regenerative properties and their possible treatment strategies for MS. Additionally, we have presented Food and Drug Administration-approved MS treatment drugs and their administration methods, mechanisms of action, safety, and effectiveness, thereby evaluating their treatment efficacy.
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http://dx.doi.org/10.3389/fphar.2021.724718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8313804PMC
July 2021

Long Non-coding RNA Uc.48+ Small Interfering RNA Alleviates Neuroinflammatory Hyperalgesia in Gp120-Treated Rats via the P2Y12 Receptor.

Front Neurosci 2021 13;15:663962. Epub 2021 Jul 13.

Neuropharmacology Laboratory of Physiology Department, Medical School of Nanchang University, Nanchang, China.

Human immunodeficiency virus envelope glycoprotein 120 (gp120) leads to hyperalgesia. Long non-coding RNAs are characterized by the lack of a protein-coding sequence and may contribute to the development and maintenance of inflammatory and neuroinflammatory pain. Rats with neuroinflammatory pain were established by gp120 treatment, which is featured by intensified pain behaviors. Long non-coding RNA uc.48+ was increased in the dorsal root ganglia of gp120-treated rats, and small interfering RNA that targets uc.48+ markedly alleviated hyperalgesia in gp120-treated rats. Notably, uc.48+ overexpression increased P2Y12 expression in control rats dorsal root ganglia and induced hyperalgesia. Uc.48+ small interfering RNA inhibited P2Y12 expression in gp120-treated rats. Uc.48+ potentiated P2Y12 receptor functions in the neurons and heterologous cells. Therefore, uc.48+ siRNA treatment reduced the upregulation of P2Y12 expression and function in DRG neurons, and, hence, alleviated hyperalgesia in gp120-treated rats.
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http://dx.doi.org/10.3389/fnins.2021.663962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315484PMC
July 2021

The effects of miR-26b-5p on fibroblast-like synovial cells in rheumatoid arthritis (RA-FLS) via targeting EZH2.

Tissue Cell 2021 Jul 15;72:101591. Epub 2021 Jul 15.

Department of Ultrasound, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, China. Electronic address:

Objective: To study the possible effects of miR-26b-5p on fibroblast-like synovial cells in rheumatoid arthritis (RA-FLS) through targeting enhancer of zeste homolog 2 (EZH2).

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect miR-26b-5p and EZH2 expressions in synovial tissues of RA patients and healthy controls. Dual luciferase reporter assay was adopted to verify the targeting relationship between miR-26b-5p and EZH2. RA-FLS was divided into Blank, mimics NC, mimics, NC siRNA, EZH2 siRNA and inhibitors + EZH2 siRNA groups, followed by the assessment of proliferation, apoptosis, migration and invasion. The expression of genes and proteins in RA-FLS was tested by qRT-PCR and western blotting, respectively.

Results: MiR-26b-5p expression was lower, while EZH2 expression was higher in synovial tissue of RA patients than healthy controls; and miR-26b-5p was negatively correlated with the EZH2 in synovial tissue of RA patients, which were both related with disease activities. MiR-26b-5p can target EZH2 in RA-FLS. In vitro, miR-26b-5p mimics down-regulated EZH2 expression in RA-FLS. Compared with EZH2 siRNA group, the miR-26b-5p expression in inhibitors + EZH2 siRNA group was reduced, but EZH2 expression was increased. EZH2 siRNA inhibited the proliferation, invasion and migration of RA-FLS, promoted cell apoptosis, and inhibited the expression of TNF-α, IL-1β, IL-6, IL-17, MMP-2, MMP-9, which were reversed by miR-26b-5p inhibitor.

Conclusion: MiR-26b-5p may affect the biological characteristics of RA-FLS via targeting EZH2, including proliferation, apoptosis, invasion and migration, as well as the secretion of cytokines, thus playing a potential therapeutic role in RA.
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http://dx.doi.org/10.1016/j.tice.2021.101591DOI Listing
July 2021

ANXA10 promotes melanoma metastasis by suppressing E3 ligase TRIM41-directed PKD1 degradation.

Cancer Lett 2021 Jul 26;519:237-249. Epub 2021 Jul 26.

The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China; Changzhou High-Tech Research Institute of Nanjing University and Jiangsu Target Pharma Laboratories Inc., Changzhou, China; School of Biopharmacy, China Pharmaceutical University, Nanjing, China. Electronic address:

Melanoma is a highly metastatic cancer that requires effective and targeted curative therapy. Annexin A10 (ANXA10), a member of the annexin family, is a calcium- and phospholipid-binding protein. Considerable evidence indicates that ANXA10 is involved in tumour progression, but little is known about its role in melanoma development. In this study, we find that ANXA10 expression is significantly upregulated, and correlates with melanoma progression. ANXA10 knockout profoundly reduces cell migration and the metastatic activity of melanoma. In addition, ANXA10 knockout induces the N- to E-cadherin switch by upregulating SMAD6, an inhibitory SMAD in the TGF-β/SMAD pathway. The negative regulation of SMAD6 by ANXA10 is dependent on PKD1. ANXA10 interacts with PKD1 and inhibits E3 ligase TRIM41-targeted PKD1 degradation. In B16F10 melanoma cells, protein levels of ANXA10 and PKD1 are inversely correlated with SMAD6 level, but correlated with cell migration. Interestingly, ANXA10 and SMAD6 levels are inversely correlated in clinical samples of melanoma progression. Our findings suggest that the ANXA10-PKD1-SMAD6 axis is a new target for therapeutic strategies against melanoma metastasis.
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http://dx.doi.org/10.1016/j.canlet.2021.07.033DOI Listing
July 2021

Dark septate endophyte Falciphora oryzae-assisted alleviation of cadmium in rice.

J Hazard Mater 2021 Jun 24;419:126435. Epub 2021 Jun 24.

Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China; State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. Electronic address:

Dark septate endophytes (DSEs) are the typical representatives of root endophytic fungi in heavy metal (HM)-contaminated environments. However, little is known about their roles in the HMs tolerance of hosts and the underlying mechanism. Here, we investigated the biological roles and molecular mechanisms of a DSE strain Falciphora oryzae in alleviating cadmium (Cd) toxicities in rice. It was found that F. oryzae possessed a capacity of accumulating Cd in its vacuoles and chlamydospores. During symbiosis, F. oryzae conferred improved Cd tolerance to rice, decreasing Cd accumulation in roots and translocation to shoots. F. oryzae alleviated Cd toxicity to rice by sequestering Cd in its vacuoles. Further application of F. oryzae as fertilizer in the field could reduce Cd content in rice grains. We identified a SNARE Syntaxin 1 gene through proteomics, which participated in Cd tolerance of F. oryzae by regulating chlamydospore formation and vacuole enlargement. This study provided novel insights into how the DSEs and their host plants combat Cd stress.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126435DOI Listing
June 2021

Structure and Interface Modification of Carbon Dots for Electrochemical Energy Application.

Small 2021 Jul 28:e2102091. Epub 2021 Jul 28.

College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.

Carbon dots (CDs) as new nanomaterials have attracted much attention in recent years due to their unique characteristics. Notably, structure and interface modification (carbon core, edge, defects, and functional groups) of CDs have been considered as valid methods to regulate their properties, which contain electron transfer effect, electrochemical activity, fluorescence luminescent, and so on. Additionally, CDs with ultrasmall size, excellent dispersibility, high specific surface area, and abundant functional groups can guarantee positive and extraordinary effects in electrical energy storage and conversion. Therefore, CDs are used to couple with other materials by constructing a special interface structure to enhance their properties. Here, diverse structural and interfacial modifications of CDs with various heteroatoms and synergy effects are systematically analyzed. And not only several main syntheses of CDs-based composites (CDs/X) are summarized but also the merit and demerit of CDs/X in electrical energy storage are discussed. Finally, the applications of CDs/X in energy storage devices (supercapacitors, batteries) and electrocatalysts for practical applications are discussed. This review mainly provides a comprehensive summary and future prospect for synthesis, modification, and electrochemical applications of CDs.
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http://dx.doi.org/10.1002/smll.202102091DOI Listing
July 2021

The national dental undergraduate clinical skills competition known as the Guanghua Cup: an innovative and effective program that promoted undergraduate dental education in China.

BMC Med Educ 2021 Jul 27;21(1):403. Epub 2021 Jul 27.

Hospital of Stomatology, Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-Sen University, 56 Lingyuan Road West, Guangzhou, 510055, Guangdong, China.

Background: The National Dental Undergraduate Clinical Skills Competition known as the Guanghua Cup was held in Guangzhou, China, for three consecutive years from 2017 to 2019 to promote the clinical teaching of undergraduate dental education and to enhance communication among different universities. The present study aimed to introduce the organization, procedures, and consequences of the competition, in addition to analyzing the influences of competition on the reform of undergraduate dental education.

Methods: By analyzing the descriptive statistics of the Guanghua Cup, the competitions' organization, the participating students' performances, and the outcomes of competitions were analyzed. After distributing questionnaires to all participants of the 2nd and 3rd Cups, their attitudes towards the competition and their evaluation of the role of the competitions in promoting undergraduate dental education were analyzed.

Results: A total of 24 schools participated in the 3 competition years. The contents of the competitions covered cariology, endodontics, periodontology, prosthodontics, oral and maxillofacial surgery, dental anatomy, and first aid (e.g., operative skills and theoretical knowledge). Compared with those of the 2nd Cup, the mean scores of the operative skills significantly improved in the stations related to periodontology, prosthodontics, and dental anatomy (p < 0.05) in the 3rd Cup. In addition, 338 valid questionnaires were collected, for a response rate of 87.79 %. Overall, the participants spoke highly of the Guanghua Cup. Based on their self-perception and self-evaluation, the majority of interviewees agreed that the competition helped develop collegiality and teamwork among the participating students, improved the students' clinical skills and promoted the improvement of teaching resources (e.g., purchasing and updating equipment, models or experimental materials).

Conclusions: The competition enjoyed the widest coverage since it involved dental schools from all of the different geographical regions of China. Dental students could exhibit their clinical skills in a competitive environment and develop collegiality and teamwork. Future competitions should be optimized through their organization and contents. The education quality of the participating schools affected by such competition should be investigated in a more objective and comparable way.
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http://dx.doi.org/10.1186/s12909-021-02841-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317344PMC
July 2021

Renal agenesis-related genes are associated with Herlyn-Werner-Wunderlich syndrome.

Fertil Steril 2021 Jul 23. Epub 2021 Jul 23.

Department of Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Chaoyang, Beijing, People's Republic of China; Beijing Maternal and Child Health Care Hospital, Chaoyang, Beijing, People's Republic of China. Electronic address:

Objective: To explore the genetic causes of Herlyn-Werner-Wunderlich syndrome (HWWS) using whole-exome sequencing.

Design: Retrospective genetic study.

Setting: Academic medical center.

Patient(s): Twelve patients with HWWS.

Intervention(s): Whole-exome sequencing was performed for each patient. Sanger sequencing was used to confirm the potential causative genetic variants. In silico analysis and American College of Medical Genetics and Genomics guidelines were used to classify the pathogenicity of each variant.

Main Outcome Measure(s): Rare sequence variants associated with müllerian duct development and renal agenesis were identified and included in subsequent analyses.

Result(s): A total of 11 variants were identified in 10 of 12 patients (83.3%) and were considered to constitute a molecular genetic diagnosis of HWWS. These 11 variants were related to 9 genes: CHD1L, TRIM32, TGFBR3, WNT4, RET, FRAS1, FAT1, FOXF1, and PCSK5. All variants were heterozygous and confirmed by Sanger sequencing. The changes included one frameshift variant, one splice-site variant, and eight missense variants. All of the identified variants were absent or rare in Genome Aggregation Database East Asian populations. One of the 11 variants (9.1%) was classified as a pathogenic variant according to the American College of Medical Genetics and Genomics guidelines, and 8 of the 11 variants (72.7%) were classified as variants of uncertain significance.

Conclusion(s): To our knowledge, this is the first report of the genetic causes of HWWS. Renal agenesis-related genes, such as CHD1L, TRIM32, RET, and WNT4, may be associated with HWWS. Identification of these variants can not only help us understand the etiology of HWWS and the relationship between reproductive tract development and urinary system development, but additionally improve the level of genetic counseling for HWWS.
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http://dx.doi.org/10.1016/j.fertnstert.2021.06.033DOI Listing
July 2021

A Bibliometric Analysis of the Top-Cited Articles on Diabetic Foot Ulcers.

Int J Low Extrem Wounds 2021 Jul 26:15347346211034388. Epub 2021 Jul 26.

Department of Endocrinology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

The aim of this study was to determine the top-cited articles in the field of diabetic foot ulcer (DFU) research. A cross-sectional bibliometric analysis was conducted in January 2021 by using Boolean search terms in the Scopus and the Web of Science databases. The 50 top-cited articles that met the inclusion criteria were ranked and evaluated for several characteristics, including year of publication, country of origin, authorship, publishing journal, topic categories, publishing type, and level of evidence. The median number of citations per article in the list was 442 (interquartile range [IQR], 320-520), with a median of 21.8 citations (IQR, 16.5-34.5) per year since publication. The publication years ranged from 1986 to 2017, with 1998 accounting for the greatest number of studies ( = 7). The citation classics were published in 20 journals and originated from institutions in 9 countries. The majority of the studies were clinical, of which expert opinion/review with Level V evidence and clinical studies with Levels I and II evidence comprised the greater proportion in the list. This study provides useful insights into the history and development of DFU research. The top-cited list may serve as a quick reference for education curriculums and clinical practice, in addition to providing a foundation for further studies on this topic.
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http://dx.doi.org/10.1177/15347346211034388DOI Listing
July 2021

Impact of basic medical insurance fund risk on the health risk assessment of urban residents.

Work 2021 Jul 16. Epub 2021 Jul 16.

Lyceum of the Philippines University, Manila, Philippines.

Background: The key to smooth running of medical insurance lies in whether the medical insurance fund can meet the needs of people's medical and health services while achieving a balance between income and expenditure. However, the current impacts of various factors make the risk of medical insurance fund more and more prominent.

Objective: This investigation is to explore the impacts of basic medical insurance fund on the health of urban residents.

Methods: The corresponding risk evaluation index system is obtained from the structure, operation process, and operation results of the medical insurance fund. The health risks of local residents are evaluated based on the Delphi method.

Results: The analysis of the influencing factors reveals that the risk of medical insurance fund is significantly correlated with the medical health of urban residents. From 2016 to 2019, the overall risk assessment for health insurance funds of the whole city is around 82 score.

Conclusion: The risk assessment system proposed can effectively assess the risks of the medical insurance fund for urban residents. This work provides an important reference for risk prevention and control of medical insurance fund management departments.
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http://dx.doi.org/10.3233/WOR-205352DOI Listing
July 2021

Prognostic and immunological significance of metastasis associated lung adenocarcinoma transcript 1 among different kinds of cancers.

Bioengineered 2021 Dec;12(1):4247-4258

Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, People's Republic of China.

LncRNAs belong to the type of noncoding RNA transcripts, which exceed 200 nucleotides in size. MALAT1 as one of the earlier identified lncRNAs in cancer is investigated by more and more scientific researchers. Expression, clinical significance and function of MALAT1 in pan-cancer exist as big difference. To detect the expression and clinical significance of MALAT1 gene precisely and comprehensively among different kinds of cancers, some classical databases such as GEPIA, TIMER, KM Plotter, and PrognoScan were fully applied. An immunological role of MALAT1 among different kinds of cancers was also determined in TIMER database. Our results showed that MALAT1 was differently expressed in different kinds of cancers using GEPIA, Oncomine, and TIMER databases to analyze. Especially, MALAT1 high RNA level was related to the early stage in lung and gastric cancer patients. MALAT1 expression was closely related to prognosis among different cancer patients. Furthermore, expression of MALAT1 was related to tumor immune cell infiltrating. Expression level of MALAT1 was also related to immune makers such as macrophage, T cell, NK cells, and so on. These findings indicate that MALAT1 could be a potential prognostic biomarker of some kinds of cancer and was significantly correlated with tumor-infiltrating immune cells in a wide variety of cancers.
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http://dx.doi.org/10.1080/21655979.2021.1955511DOI Listing
December 2021

Characteristics and Safety of CO for the Fire Prevention Technology with Gob-Side Entry Retaining in Goaf.

ACS Omega 2021 Jul 8;6(28):18518-18526. Epub 2021 Jul 8.

School of Economics and Management, North China Institute of Science and Technology, Beijing 101601, China.

The mining technology of gob-side entry retaining without a coal pillar is gradually becoming a mature and increasingly important mining technology. As it maintains the roadway near goaf, the air leakage should be greater than a U-type ventilation system in goaf, so it is prone to cause coal spontaneous combustion problems. CO is an inert gas, and it is usually used to prevent spontaneous combustion and extinguish coal fire. However, there is a lack of research on characteristics and safety of CO for the mining technology of gob-side entry retaining without the coal pillar. In this paper, the indexes of influencing factors were proposed on gas, pipelines, and mining technical parameters. Using a three-dimensional physical model of coal stope, the gas migration law of CO, the relationship between gas injection rate and the oxidation zone area, and the safety of the CO inerting technology were analyzed. The results indicate that the O concentration is diluted between the working face and the injection port, especially in the air intake side. Furthermore, the CO injection rate is an important parameter to the fire prevention and extinguishing technology. When the gas injection rate ranges from 240 to 720 m/h, the oxidation zone area varies from 7380 to 14 760 m, and the gas injection rate grows exponentially with the area of the oxidation zone. Moreover, the redundant CO gas flows to the retaining roadway, and it reduces the O concentration, resulting in asphyxia accidents of miners. The research results are helpful to balance the relationship between inert gas injection and production safety and provide guidance for the practical application of the inert gas fire prevention technology.
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http://dx.doi.org/10.1021/acsomega.1c02836DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296586PMC
July 2021

MicroRNA-181b Serves as a Circulating Biomarker and Regulates Inflammation in Heart Failure.

Dis Markers 2021 1;2021:4572282. Epub 2021 Jul 1.

Department of Cardiology, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street Harbin, Heilongjiang, China 150001.

Heart failure (HF) is the typical terminal stage of cardiac diseases involving inflammatory states. The function of microRNAs (miRNAs) in the progress of HF remains poorly understood. In this study, real-time PCR results showed a decreased expression of miRNA-181b (miR-181b) in HF patients compared with healthy individuals. Besides, miR-181b expressions were negatively correlated with hypersensitive C-reactive protein (hsCRP) levels in the serum of HF patients. Receiver operator characteristic (ROC) curve analysis showed that miR-181b was a diagnostic predictor of HF, and the area under the curve was 0.970 (DCM-induced HF group) and 0.962 (ICM-induced HF group). Strikingly, in HF rats induced by isoproterenol (ISO), the expression of miR-181b of heart tissue was suppressed before tumor necrosis factor-alpha (TNF-), interleukin-1 (IL-1), and interleukin-6 (IL-6) increase, as revealed by western blot and real-time PCR. Besides, the overexpression of miR-181b also decreased the expression of TNF-, IL-1, and IL-6 in lipopolysaccharide- (LPS-) induced neonatal cardiomyocytes. In conclusion, our results revealed that miR-181b might be a potential biomarker for HF and provided a novel target for anti-inflammatory therapy.
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http://dx.doi.org/10.1155/2021/4572282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270725PMC
July 2021
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