Publications by authors named "Li Jing"

10,961 Publications

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Absolute configuration of polypropionate derivatives: Decempyrones A-J and their MptpA inhibition and anti-inflammatory activities.

Bioorg Chem 2021 Jul 19;115:105156. Epub 2021 Jul 19.

School of Marine Sciences, Sun Yat-sen University, Guangzhou 510006, China; Southern Laboratory of Ocean Science and Engineering (Guangdong, Zhuhai), Zhuhai 519000, China; Pearl River Estuary Marine Ecosystem Research Station, Ministry of Education, Zhuhai 519082, China. Electronic address:

Under guidance of H NMR, ten new polypropionate derivatives, decempyrones A-J (1-10) along with two known analogues (11 and 12), were isolated from the marine-derived fungusFusarium decemcellulare SYSU-MS6716. The planar structures were elucidated on the basis of extensive spectroscopic analyses (1D and 2D NMR, and HR-ESIMS). The absolute configuration of the chiral centers in the side chain is a major obstacle for the structure identification of natural polypropionate derivatives. Herein, the J-based configurational analysis (JBCA), chemical degradation, geminal proton rule, and the modified Mosher's method were adopted to fix their absolute configurations in the side chain. Compounds 3 and 10 exhibited potent anti-inflammatory activity by inhibiting the production of NO in RAW264.7 cells activated by lipopolysaccharide with ICvalues 22.4 ± 1.8 and 21.7 ± 1.1 μM. In addition, compounds 3 and 10 displayed MptpA inhibitory activity with an IC value of 19.2 ± 0.9 and 33.1 ± 2.9 µM. Structure-activity relationships of the polypropionate derivatives were discussed.
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http://dx.doi.org/10.1016/j.bioorg.2021.105156DOI Listing
July 2021

Spatial associations between tree regeneration and soil nutrient in secondary forest in Guandi Mountains, Shanxi, China.

Ying Yong Sheng Tai Xue Bao 2021 Jul;32(7):2363-2370

College of Forestry, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China.

According to the technical specifications of CTFS (Center for Tropical Forest Science), 190 sampling points of 4-hm plot (GDS plot) in Guandi Mountains of Shanxi Province, China were investigated to examine the spatial distribution pattern of seedlings and 14 soil indicators, including pH value and nutrient indicators. We analyzed the spatial associations between tree regeneration and soil nutrients, explored the mechanism of tree regeneration pattern of secon-dary forest. The results showed that more seedlings survived in the patches with low contents of available N, available K, available Cu, available Fe, available Mn, available Ni and available Zn. Besides available K, the negative correlation between the other six soil nutrient factors and the number of regeneration seedlings of the Ⅱ (2.5 cm
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http://dx.doi.org/10.13287/j.1001-9332.202107.006DOI Listing
July 2021

The let-7f-5p-Nme4 pathway mediates tumor necrosis factor α-induced impairment in osteogenesis of bone marrow-derived mesenchymal stem cells.

Biochem Cell Biol 2021 Jul 23:1-11. Epub 2021 Jul 23.

Department of Orthopaedics, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.

Although tumor necrosis factor α (TNF-α)-mediated inflammation significantly impacts osteoporosis, the mechanisms underlying the osteogenic differentiation defects of bone marrow-derived mesenchymal stem cells (BM-MSCs) caused by TNF-α remain poorly understood. We found that TNF-α stimulation of murine BM-MSCs significantly upregulated the expression levels of several microRNAs (miRNAs), including let-7f-5p, but this increase was significantly reversed by treatment with the kinase inhibitor BAY 11-7082. To study gain- or loss of function, we transfected cells with an miRNA inhibitor or miRNA mimic. We then demonstrated that let-7f-5p impaired osteogenic differentiation of BM-MSCs in the absence and presence of TNF-α, as evidenced by alkaline phosphatase and alizarin red staining as well as quantitative assays of the mRNA levels of bone formation marker genes in differentiated BM-MSCs. Moreover, let-7f-5p targets the 3' untranslated region of Nucleoside diphosphate kinase 4 (Nme4) mRNA and negatively regulates Nme4 expression in mouse BM-MSCs. Ectopic expression of Nme4 completely reversed the inhibitory effects of the let-7f-5p mimic on osteogenic differentiation of mouse BM-MSCs. Furthermore, inhibition of let-7f-5p or overexpression of Nme4 in BM-MSCs restored in-vivo bone formation in an ovariectomized animal model. Collectively, our work indicates that let-7f-5p is involved in TNF-α-mediated reduction of BM-MSC osteogenesis via targeting Nme4.
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http://dx.doi.org/10.1139/bcb-2020-0281DOI Listing
July 2021

Emerging Dual-Atomic-Site Catalysts for Efficient Energy Catalysis.

Adv Mater 2021 Jul 23:e2102576. Epub 2021 Jul 23.

School of Materials Science & Engineering, and College of Engineering, Peking University, Beijing, 100871, China.

Atomically dispersed metal catalysts with well-defined structures have been the research hotspot in heterogeneous catalysis because of their high atomic utilization efficiency, outstanding activity, and selectivity. Dual-atomic-site catalysts (DASCs), as an extension of single-atom catalysts (SACs), have recently drawn surging attention. The DASCs possess higher metal loading, more sophisticated and flexible active sites, offering more chance for achieving better catalytic performance, compared with SACs. In this review, recent advances on how to design new DASCs for enhancing energy catalysis will be highlighted. It will start with the classification of marriage of two kinds of single-atom active sites, homonuclear DASCs and heteronuclear DASCs according to the configuration of active sites. Then, the state-of-the-art characterization techniques for DASCs will be discussed. Different synthetic methods and catalytic applications of the DASCs in various reactions, including oxygen reduction reaction, carbon dioxide reduction reaction, carbon monoxide oxidation reaction, and others will be followed. Finally, the major challenges and perspectives of DASCs will be provided.
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http://dx.doi.org/10.1002/adma.202102576DOI Listing
July 2021

Zero-Valent Palladium Single-Atoms Catalysts Confined in Black Phosphorus for Efficient Semi-Hydrogenation.

Adv Mater 2021 Jul 23:e2008471. Epub 2021 Jul 23.

Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore.

Single-atom catalysts (SACs) represent a new frontier in heterogeneous catalysis due to their remarkable catalytic properties and maximized atomic utilization. However, single atoms often bond to the support with polarized electron density and thus exhibit a high valence state, limiting their catalytic scopes in many chemical transformations. Here, it is demonstrated that 2D black phosphorus (BP) acts as giant phosphorus (P) ligand to confine a high density of single atoms (e.g., Pd , Pt ) via atomic layer deposition. Unlike other 2D materials, BP with relatively low electronegativity and buckled structure favors the strong confinement of robust zero-valent palladium SACs in the vacancy site. Metallic Pd /BP SAC shows a highly selective semi-hydrogenation of phenylacetylene toward styrene, distinct from metallic Pd nanoparticles that facilitate the formation of fully hydrogenated products. Density functional theory calculations reveal that Pd atom forms covalent-like bonding with adjacent P atoms, wherein H atoms tend to adsorb, aiding the dissociative adsorption of H . Zero-valent Pd in the confined space favors a larger energy gain for the synthesis of partially hydrogenated product over the fully hydrogenated one. This work provides a new route toward the synthesis of zero-valent SACs on BP for organic transformations.
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http://dx.doi.org/10.1002/adma.202008471DOI Listing
July 2021

Selenium attenuates ischemia/reperfusion injury‑induced damage to the blood‑brain barrier in hyperglycemia through PI3K/AKT/mTOR pathway‑mediated autophagy inhibition.

Int J Mol Med 2021 Sep 23;48(3). Epub 2021 Jul 23.

Ningxia Key Laboratory of Cerebrocranial Diseases, School of Basic Medical Science, Incubation Base of National Key Laboratory, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China.

Ischemic stroke is a leading cause of mortality and disability. Diabetes mellitus, characterized by hyperglycemia, is a common concomitant disease of ischemic stroke, which is associated with autophagy dysfunction and blood‑brain barrier (BBB) damage following cerebral ischemia/reperfusion (I/R) injury. At present, there is no effective treatment strategy for the disease. The purpose of the present study was to explore the molecular mechanisms underlying the protective effects of selenium on the BBB following I/R injury in hyperglycemic rats. Middle cerebral artery occlusion was performed in diabetic Sprague‑Dawley rats. Treatment with selenium and the autophagy inhibitor 3‑methyladenine significantly reduced cerebral infarct volume, brain water content and Evans blue leakage, while increasing the expression of tight junction (TJ) proteins and decreasing that of autophagy‑related proteins (P<0.05). In addition, selenium increased the phosphorylation levels of PI3K, AKT and mTOR (P<0.05). A mouse bEnd.3 brain microvascular endothelial cell line was co‑cultured with an MA‑h mouse astrocyte‑hippocampal cell line to simulate the BBB. The cells were then subjected to hyperglycemia, followed by oxygen‑glucose deprivation for 1 h and reoxygenation for 24 h. It was revealed that selenium increased TJ protein levels, reduced BBB permeability, decreased autophagy levels and enhanced the expression of phosphorylated (p)‑AKT/AKT and p‑mTOR/mTOR proteins (P<0.05). Treatment with wortmannin (an inhibitor of PI3K) significantly prevented the beneficial effects of selenium on the BBB, whereas insulin‑like growth factor 1 (a PI3K activator) mimicked the effects of selenium. In conclusion, the present findings indicated that selenium can inhibit autophagy by regulating the PI3K/AKT/mTOR signaling pathway, significantly preventing BBB damage following cerebral I/R injury in hyperglycemic conditions.
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http://dx.doi.org/10.3892/ijmm.2021.5011DOI Listing
September 2021

Unresponsive thin endometrium caused by Asherman syndrome treated with umbilical cord mesenchymal stem cells on collagen scaffolds: a pilot study.

Stem Cell Res Ther 2021 Jul 22;12(1):420. Epub 2021 Jul 22.

Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, NO.3 Qingchun East Road, Shangcheng District, Hangzhou, 310016, People's Republic of China.

Background: Unresponsive thin endometrium caused by Asherman syndrome (AS) is the major cause of uterine infertility. However, current therapies are ineffective. This study is to evaluate the effect of transplantation with collagen scaffold/umbilical cord mesenchymal stem cells (CS/UC-MSCs) on this refractory disease.

Methods: Eighteen infertile women with unresponsive thin endometrium, whose frozen-thawed embryo transfers (FETs) were cancelled due to reduced endometrial thickness (ET ≤ 5.5 mm), were enrolled in this before and after self-control prospective study. Hysteroscopic examination was performed to confirm no intrauterine adhesions, then twenty million UC-MSCs loaded onto a CS were transplanted into the uterine cavity in two consecutive menstrual cycles. Then uterine cavity was assessed through hysteroscopy after two transplants. FETs were performed in the following cycle. Pregnancy outcomes were followed up. Endometrial thickness, uterine receptivity and endometrial angiogenesis, proliferation and hormone response were compared before and after treatment.

Results: Sixteen patients completed the study. No treatment-related serious adverse events occurred. Three months after transplantation, the average ET increased from 4.08 ± 0.26 mm to 5.87 ± 0.77 mm (P < 0.001). Three of 15 patients after FET got pregnant, of whom 2 gave birth successfully and 1 had a miscarriage at 25 weeks' gestation. One of 2 patients without FET had a natural pregnancy and gave birth normally after transplantation. Immunohistochemical analysis showed increased micro-vessel density, upregulated expression of Ki67, estrogen receptor alpha, and progesterone receptor, indicating an improvement in endometrial angiogenesis, proliferation, and response to hormones.

Conclusion: CS/UC-MSCs is a promising and potential approach for treating women with unresponsive thin endometrium caused by AS.

Trial Registration: ClinicalTrials.gov NCT03724617 . Registered on 26 October 2018-prospectively registered, https://register.clinicaltrials.gov/.
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http://dx.doi.org/10.1186/s13287-021-02499-zDOI Listing
July 2021

Effects of 5.8 GHz microwave on hippocampal synaptic plasticity of rats.

Int J Environ Health Res 2021 Jul 22:1-13. Epub 2021 Jul 22.

Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, Xi'an, Shaanxi, China.

5.8 GHz spectrum is gaining more attention in wireless technology. To explore the potential hazards, we investigated the effect of exposure to 5.8 GHz microwave on learning and memory ability of rats. Morris Water maze (MWM), Novel object recognition (NOR) and Fear conditioning test (FCT) were used to evaluate the ability of spatial and non-spatial memory of rats. The hippocampal morphology, the level of brain injury factors in serum and the mitochondrial membrane potential of hippocampal neurons was examined to evaluate the damage of hippocampal neurons. The density of dendritic spines, the ultrastructure of synapses and the level of PSD95, Synaptophysin, p-CREB and CREB were detected to evaluate the hippocampal synaptic plasticity. Compared with Sham group, there was no significant difference in the performance of ethology (in MWM, NOR, FCT) in Microwave 2 h group or Microwave 4 h group. The hippocampal morphology, the serum level of brain injury factors and the content of mitochondrial JC-1 monomer in Microwave 2 h group or Microwave 4 h group did not change obviously, compared with Sham group. The density of dendritic spines, the ultrastructure of synapse and the level of PSD95, Synaptophysin, p-CREB and CREB in hippocampus in Microwave 2 h group or Microwave 4 h group did not significantly change, compared with Sham group. Under this experimental condition, exposure to 5.8 GHz microwave could not affect the hippocampal synaptic plasticity of rats.
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http://dx.doi.org/10.1080/09603123.2021.1952165DOI Listing
July 2021

SuperDTI: Ultrafast DTI and fiber tractography with deep learning.

Magn Reson Med 2021 Jul 26. Epub 2021 Jul 26.

Electrical Engineering, University at Buffalo, State University of New York, Buffalo, New York, USA.

Purpose: To develop a deep learning-based reconstruction framework for ultrafast and robust diffusion tensor imaging and fiber tractography.

Methods: SuperDTI was developed to learn the nonlinear relationship between DWIs and the corresponding diffusion tensor parameter maps. It bypasses the tensor fitting procedure, which is highly susceptible to noises and motions in DWIs. The network was trained and tested using data sets from the Human Connectome Project and patients with ischemic stroke. Results from SuperDTI were compared against widely used methods for tensor parameter estimation and fiber tracking.

Results: Using training and testing data acquired using the same protocol and scanner, SuperDTI was shown to generate fractional anisotropy and mean diffusivity maps, as well as fiber tractography, from as few as six raw DWIs, with a quantification error of less than 5% in all white-matter and gray-matter regions of interest. It was robust to noises and motions in the testing data. Furthermore, the network trained using healthy volunteer data showed no apparent reduction in lesion detectability when directly applied to stroke patient data.

Conclusions: Our results demonstrate the feasibility of superfast DTI and fiber tractography using deep learning with as few as six DWIs directly, bypassing tensor fitting. Such a significant reduction in scan time may allow the inclusion of DTI into the clinical routine for many potential applications.
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http://dx.doi.org/10.1002/mrm.28937DOI Listing
July 2021

A benchmarking study of SARS-CoV-2 whole-genome sequencing protocols using COVID-19 patient samples.

iScience 2021 Jul 21:102892. Epub 2021 Jul 21.

Center for Genomics, School of Medicine, Loma Linda University, Loma Linda, California, United States of America.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emerging new type of coronavirus that is responsible for the COVID-19 pandemic and the unprecedented global health emergency. Whole-genome sequencing (WGS) of SARS-CoV-2 plays a critical role in understanding the disease. Performance variation exists across SARS-CoV-2 viral WGS technologies, but there is currently no benchmarking study comparing different WGS sequencing protocols. We compared seven different SARS-CoV-2 WGS library protocols using RNA from patient nasopharyngeal swab samples under two storage conditions with low and high viral inputs. We found large differences in mappability and genome coverage, and variations in sensitivity, reproducibility and precision of single-nucleotide variant calling across different protocols. For certain amplicon-based protocol, appropriate primer trimming step is critical for accurate single-nucleotide variant calling. We ranked the performance of protocols based on six different metrics. Our findings offer guidance in choosing appropriate WGS protocols to characterize SARS-CoV-2 and its evolution.
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http://dx.doi.org/10.1016/j.isci.2021.102892DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294598PMC
July 2021

Sacral chondroblastoma - a rare location, a rare pathology: A case report and review of literature.

World J Clin Cases 2021 Jul;9(20):5709-5716

Department of Spine Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China.

Background: Chondroblastoma (CB) is an intermediate tumor of cartilage origin. CB involving the sacrum is a very rare pathology.

Case Summary: A 17-year-old male with sacral CB was diagnosed as CB during the first surgery, and 18 mo later, the tumor recurred and a second surgery was performed with the same pathology result of CB.

Conclusion: We recommend complete removal of the tumor in a timely manner, provided that surgical conditions are met. At the same time, other diseases should be carefully differentiated in terms of imaging or pathological features so as to avoid erroneous diagnostic conclusions.
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http://dx.doi.org/10.12998/wjcc.v9.i20.5709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281409PMC
July 2021

Peach- intercropping mode affects soil properties and fungal composition.

PeerJ 2021 12;9:e11705. Epub 2021 Jul 12.

Horticulture Research Institute, Sichuan Academy of Agricultural Sciences & Key Laboratory of Horticultural Crop Biology and Germplasm Creation in Southwestern China of the Ministry of Agriculture and Rural Affairs, Chengdu, China.

Objective: This study aims to explore a three-dimensional planting mode in orchards and provide theoretical basis for the efficient peach- planting and soil management after cultivation.

Methods: Next-generation sequencing was performed to investigate the variations in soil physicochemical properties, enzyme activities and fungal composition under peach- intercropping for one year and two years, by using the soil without peach- intercropping as the control group.

Results: Peach- intercropping decreased the soil bulk density, and significantly increased the maximum field capacity, non-capillary porosity and total porosity, organic matter, available potassium and available zinc, which together improved soil structure and soil fertility. Besides, the intercropping mode obviously enhanced soil enzyme activities and mineral absorption and transformation in peach orchard soils. The intercropping also resulted in a decline of soil fungal diversity, and the 2-year soil samples were of higher abundance of . More importantly, peach- intercropping elevated the yields of both peach and , bringing about obviously higher economic benefits.

Conclusion: Continuous peach- intercropping improves the soil structure and fertility while decreases soil fungal diversity, which can contribute to greater economic benefits of the peach orchard. Our findings shed new light on the intercropping-fungus-soil relationship, and may facilitate the further development of peach- intercropping.
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http://dx.doi.org/10.7717/peerj.11705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280869PMC
July 2021

Cost-Effectiveness Analysis of a Mobile-Based Intervention for Patients with Type 2 Diabetes Mellitus.

Int J Endocrinol 2021 1;2021:8827629. Epub 2021 Jul 1.

National Health Commission Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, No 6, Huanruibei Road, Beichen District, Tianjin 300134, China.

Objective: The aim of this study was to evaluate the cost effectiveness of a mobile-based intervention for patients with type 2 diabetes mellitus (T2DM) and compare it with the usual management mode.

Method: A total of 215 patients with T2DM in a tertiary-care hospital specific to diabetes were selected as the study population. This study was conducted from January 1, 2019 to January 1, 2020. Of the 215 patients, 130 were randomly assigned to the mHealth group and 85 were assigned to the usual care group. IBM SPSS 25.0 software was used for descriptive statistics, tests, chi-square tests, and correlation analyses. Haemoglobin A1c (HbA1c) was the effectiveness parameter adopted. Cost-effectiveness analyses were performed, and incremental cost-effectiveness ratios (ICERs) were calculated.

Results: Of the 215 patients with T2DM, the proportion of male patients was 66.0%. The mean age of the patients was 47.2 (SD 9.95). Differences in baseline information were not statistically significant between the two groups ( > 0.05). At the 3-, 6-, and 12-month follow-ups, the mHealth group reported higher control rates of HbA1c than the usual care group, 67.9% versus 46.2% ( < 0.001), 72.4% versus 45.4% ( < 0.001), and 74.6% versus 47.1% ( < 0.001), respectively. The value of HbA1c was positively related to total patient cost, material fee, Western medicine fee, and hospitalization expenses ( < 0.05), with correlation coefficients of 0.202, 0.200, 0.172, and 0.183, respectively. The costs of the mHealth group and usual care group were CNY¥ 1169.76 and CNY¥ 1775.44 per patient/year, respectively. The incremental cost of the mHealth intervention was CNY¥ -605.68 per patient/year. The ICER was CNY¥ -22.02 per patient/year.

Conclusion: Compared with the usual care mode, the mHealth management model for patients with T2DM improved the control rate of HbA1c, and the mHealth management mode had better cost effectiveness.
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http://dx.doi.org/10.1155/2021/8827629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266460PMC
July 2021

Maternal dietary patterns are associated with human milk composition in Chinese lactating women.

Nutrition 2021 Jun 23;91-92:111392. Epub 2021 Jun 23.

Department of Maternal, Child and Adolescent Health, School of Public Health, Nanjing Medical University, Nanjing, China.

Objectives: Dietary patterns are a useful tool to study the impact of overall maternal diet on human milk (HM) composition beyond single foods or nutrients. The present study aimed to identify dietary patterns among Chinese lactating women and assess their associations with HM macronutrient composition.

Methods: Dietary intake data and HM samples were collected from 122 Chinese mothers at three to five study visits during the first 52 d postpartum. Dietary patterns were derived using principal component analysis. Cross-sectional associations of dietary patterns and HM macronutrients were assessed using multivariable linear regression models adjusted for total energy intake. All analyses were done separately for colostrum (postpartum days 0-7) and mature milk (postpartum days 8-52).

Results: Four dietary patterns were identified: high-in-animal-foods, high-in-eggs, high-in-plant-foods, and high-in-fruits. Compared with the lowest tertile (T1), participants in the highest tertile (T3) of the high-in-animal-foods and high-in-plant-foods patterns had lower protein (respectively, T3 - T1 = -1.09 g/100 mL, P = 0.002; T3 - T1 = -0.54 g/100 mL, P = 0.001) and higher fat (respectively, T3 - T1 = 0.86 g/100 mL, P = 0.040; T3 - T1 = 0.40 g/100 mL, P = 0.004) concentrations in colostrum. In contrast, in mature milk the high-in-animal-foods pattern was positively associated with carbohydrates (T3 - T1 = 0.53 g/100 mL, P = 0.008) and the high-in-plant-foods pattern was negatively associated with fat (T3 - T1 = -0.64 g/100 mL, P = 0.002). The high-in-eggs pattern was weakly positively associated with protein concentration in mature milk (T3 - T1 = 0.10 g/100 mL, P = 0.023).

Conclusions: Maternal dietary patterns with high proportions of animal and plant-based foods were associated with higher fat and lower protein concentrations in colostrum. Different associations were found in mature milk. Dietary-pattern analysis provides an opportunity to characterize total diet and may be more predictive of HM composition than single foods or nutrients.
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http://dx.doi.org/10.1016/j.nut.2021.111392DOI Listing
June 2021

Association between long-term exposure to ambient air pollution and clinical outcomes among patients with heart failure: Findings from the China PEACE Prospective Heart Failure Study.

Ecotoxicol Environ Saf 2021 Jul 22;222:112517. Epub 2021 Jul 22.

National Clinical Research Center for Cardiovascular Diseases, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China. Electronic address:

Background: The health effects of air pollution on heart failure (HF) patients have not been adequately studied.

Objectives: We assessed the associations between long-term air pollution exposure and prognosis in HF patients.

Methods: HF patients were prospectively recruited from 52 hospitals throughout China between August 2016 and May 2018. The participants were followed up for 12 months after discharge from index hospitalization. Long-term air pollution was calculated as annual average level of air pollution before the date of the index hospitalization. Outcomes were defined as HF readmission, cardiovascular death, and composite events. Cox proportional hazards model was applied to quantify the associations between air pollution exposure and clinical outcomes.

Results: Of 4866 patients included in the analysis, mean age was 65.2 ± 13.5 years, and 62.5% were male. During 1-year follow-up, 1577 (32.4%) participants were readmitted for HF and 550 (11.3%) died from cardiovascular disease. Though no associations between long-term air pollution and HF readmission in the overall participants, geographic and age heterogeneity in the long-term effects of air pollutants on HF readmission was observed. Air pollutants included PM [HR (hazard ratio) = 1.146, 95% CI (confidence interval): 1.044, 1.259], PM (HR = 1.120, 95% CI: 1.043, 1.203), SO (HR = 1.808, 95% CI: 1.190, 2.747), and CO (HR = 3.596, 95% CI: 1.792, 7.218) were associated with higher risk of HF readmission in South China, but not in North China, where people spend less time outdoors and have limited indoor-outdoor ventilation. PM, PM O, and CO among patients ≥ 65 years were found to be associated with higher risk of HF readmission. The effects on composite outcomes were broadly consistent with that of HF readmission. Cardiovascular death was not significantly associated with air pollution in the overall or subgroups.

Discussion: Among HF patients who were older, living in South China, more HF readmissions occurred with higher long-term air pollution exposure. The findings suggest that the elderly patients and those living in South China should particularly enhance their personal protection against air pollution.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112517DOI Listing
July 2021

Engineering Ruthenium-Based Electrocatalysts for Effective Hydrogen Evolution Reaction.

Nanomicro Lett 2021 Jul 24;13(1):160. Epub 2021 Jul 24.

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Provincial Key Lab of Fine Chemistry, School of Chemical Engineering and Technology, Hainan University, Haikou, 570228, People's Republic of China.

The investigation of highly effective, durable, and cost-effective electrocatalysts for the hydrogen evolution reaction (HER) is a prerequisite for the upcoming hydrogen energy society. To establish a new hydrogen energy system and gradually replace the traditional fossil-based energy, electrochemical water-splitting is considered the most promising, environmentally friendly, and efficient way to produce pure hydrogen. Compared with the commonly used platinum (Pt)-based catalysts, ruthenium (Ru) is expected to be a good alternative because of its similar hydrogen bonding energy, lower water decomposition barrier, and considerably lower price. Analyzing and revealing the HER mechanisms, as well as identifying a rational design of Ru-based HER catalysts with desirable activity and stability is indispensable. In this review, the research progress on HER electrocatalysts and the relevant describing parameters for HER performance are briefly introduced. Moreover, four major strategies to improve the performance of Ru-based electrocatalysts, including electronic effect modulation, support engineering, structure design, and maximum utilization (single atom) are discussed. Finally, the challenges, solutions and prospects are highlighted to prompt the practical applications of Ru-based electrocatalysts for HER.
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http://dx.doi.org/10.1007/s40820-021-00679-3DOI Listing
July 2021

neoDL: a novel neoantigen intrinsic feature-based deep learning model identifies IDH wild-type glioblastomas with the longest survival.

BMC Bioinformatics 2021 Jul 23;22(1):382. Epub 2021 Jul 23.

Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Engineering Medicine, School of Biological Science and Medical Engineering, Beihang University, No.37 Xueyuan Road, Haidian District, Beijing, 100083, People's Republic of China.

Background: Neoantigen based personalized immune therapies achieve promising results in melanoma and lung cancer, but few neoantigen based models perform well in IDH wild-type GBM, and the association between neoantigen intrinsic features and prognosis remain unclear in IDH wild-type GBM. We presented a novel neoantigen intrinsic feature-based deep learning model (neoDL) to stratify IDH wild-type GBMs into subgroups with different survivals.

Results: We first derived intrinsic features for each neoantigen associated with survival, followed by applying neoDL in TCGA data cohort(AUC = 0.988, p value < 0.0001). Leave one out cross validation (LOOCV) in TCGA demonstrated that neoDL successfully classified IDH wild-type GBMs into different prognostic subgroups, which was further validated in an independent data cohort from Asian population. Long-term survival IDH wild-type GBMs identified by neoDL were found characterized by 12 protective neoantigen intrinsic features and enriched in development and cell cycle.

Conclusions: The model can be therapeutically exploited to identify IDH wild-type GBM with good prognosis who will most likely benefit from neoantigen based personalized immunetherapy. Furthermore, the prognostic intrinsic features of the neoantigens inferred from this study can be used for identifying neoantigens with high potentials of immunogenicity.
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http://dx.doi.org/10.1186/s12859-021-04301-6DOI Listing
July 2021

Altered speech patterns in subjects with post-traumatic headache due to mild traumatic brain injury.

J Headache Pain 2021 Jul 23;22(1):82. Epub 2021 Jul 23.

School of Electrical, Computer and Energy Engineering and College of Health Solutions, Arizona State University, Tempe, AZ, USA.

Background/objective: Changes in speech can be detected objectively before and during migraine attacks. The goal of this study was to interrogate whether speech changes can be detected in subjects with post-traumatic headache (PTH) attributed to mild traumatic brain injury (mTBI) and whether there are within-subject changes in speech during headaches compared to the headache-free state.

Methods: Using a series of speech elicitation tasks uploaded via a mobile application, PTH subjects and healthy controls (HC) provided speech samples once every 3 days, over a period of 12 weeks. The following speech parameters were assessed: vowel space area, vowel articulation precision, consonant articulation precision, average pitch, pitch variance, speaking rate and pause rate. Speech samples of subjects with PTH were compared to HC. To assess speech changes associated with PTH, speech samples of subjects during headache were compared to speech samples when subjects were headache-free. All analyses were conducted using a mixed-effect model design.

Results: Longitudinal speech samples were collected from nineteen subjects with PTH (mean age = 42.5, SD = 13.7) who were an average of 14 days (SD = 32.2) from their mTBI at the time of enrollment and thirty-one HC (mean age = 38.7, SD = 12.5). Regardless of headache presence or absence, PTH subjects had longer pause rates and reductions in vowel and consonant articulation precision relative to HC. On days when speech was collected during a headache, there were longer pause rates, slower sentence speaking rates and less precise consonant articulation compared to the speech production of HC. During headache, PTH subjects had slower speaking rates yet more precise vowel articulation compared to when they were headache-free.

Conclusions: Compared to HC, subjects with acute PTH demonstrate altered speech as measured by objective features of speech production. For individuals with PTH, speech production may have been more effortful resulting in slower speaking rates and more precise vowel articulation during headache vs. when they were headache-free, suggesting that speech alterations were related to PTH and not solely due to the underlying mTBI.
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http://dx.doi.org/10.1186/s10194-021-01296-6DOI Listing
July 2021

Evaluation of coastal farming under salinization and optimized fertilization strategies in China.

Sci Total Environ 2021 Jul 14;797:149038. Epub 2021 Jul 14.

Yellow River Delta Modern Agricultural Engineering Laboratory, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China.

Nitrogen (N) application and salinity are key factors influencing crop yield and net economic benefit in coastal saline-alkali soils. Integrated analysis and optimization of the benefits of wheat-corn cropping under different nitrogen applications in saline soils could provide lay the scientific basis for sustainable development of agriculture in coastal farmlands. A total of 571 pair-reviewed literature data on two-factor cross-over trials, involving soil salinity and nitrogen application, were integratively analyzed. Based on multi-objective optimization of crop yield, agronomic and net economic benefits, and soil nitrate residue in coastal saline-alkali soils, area-specific nitrogen application strategies were developed. The results showed that increasing the N application rate under 1.8-2.9‰ salinity can increase crop yield and economic benefits. The net economic benefit of crops was negative under 3.5‰ salinity. Above that threshold of 3.5‰, it is not suitable for planting food crops. Consequently, it is necessary to strengthen the management of saline-alkali soils. While the application rate of 2.78 × 10 kg N in winter wheat/summer corn cropping ensured environmental protection, farmers preferred 3.08 × 10 kg of nitrogen dose. These were respectively 40.4% and 33.9% lower than the traditional dose and with relatively higher benefits too.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149038DOI Listing
July 2021

Oxidative stress and endoplasmic reticulum stress contributed to hepatotoxicity of decabromodiphenyl ethane (DBDPE) in L-02 cells.

Chemosphere 2021 Jul 13;286(Pt 1):131550. Epub 2021 Jul 13.

School of Public Health and Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, 100069, China. Electronic address:

Decabromodiphenyl ethane (DBDPE) is one of the most commonly used novel brominated flame retardants (NBFRs), and its mass production and widespread application have caused health threats to the human being. Existing studies have shown that DBDPE has hepatotoxicity. And we have found that DBDPE could change cytochrome P450 3A (CYP3A) expression levels in rat livers, whereas the mechanism is unclear. In this study, we exposed human normal hepatocyte (L-02) to DBDPE to further study the effect and mechanism of DBDPE on hepatocellular injury and liver metabolic enzyme CYP3A changes in vitro. The results showed that DBDPE caused L-02 cell viability decrease, lactate dehydrogenase (LDH) and transaminase release, ultrastructural damage, and apoptosis. Moreover, DBDPE exposure induced oxidative stress (i.e., increased ROS generation and MDA levels and decreased GSH content, SOD activity, and mitochondrial membrane potential) and endoplasmic reticulum (ER) stress in L-02 cells as evidenced by the elevated PERK and IRE-1α expression levels. These results confirmed that DBDPE is toxic to hepatocytes. Besides, the CYP3A expression level was decreased in DBDPE exposed L-02 cells. However, pretreatment of L-02 cells with antioxidant N-Acetyl-l-cysteine (NAC) and endoplasmic reticulum stress inhibitor 4-PBA inhibited DBDPE-induced oxidative stress, endoplasmic reticulum stress, CYP3A expression decrease, and apoptosis. Therefore, we demonstrated that DBDPE could exert toxic effects and decrease CYP3A expression on L-02 cells by inducing ER stress and oxidative stress.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131550DOI Listing
July 2021

Facile Production of Phosphorene Nanoribbons towards Application in Lithium Metal Battery.

Adv Mater 2021 Jul 22:e2102083. Epub 2021 Jul 22.

Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore.

Like phosphorene, phosphorene nanoribbon (PNR) promises exotic properties but unzipping phosphorene into edge-defined PNR is non-trivial because of uncontrolled cutting of phosphorene along random directions. Here a facile electrochemical strategy to fabricate zigzag-edged PNRs in high yield (>80%) is reported. The presence of chemically active zigzag edges in PNR allows it to spontaneously react with Li to form a Li ion conducting Li P phase, which can be used as a protective layer on Li metal anode in lithium metal batteries (LMBs). PNR protective layer prevents the parasitic reaction between lithium metal and electrolyte and promotes Li ion diffusion kinetics, enabling homogenous Li ion flux and long-time cycling stability up to 1100 h at a current density of 1 mA cm . LiFePO |PNR-Li full-cell batteries with an areal capacity of 2 mAh cm , a lean electrolyte (20 µl mAh ) and a negative/positive (N/P) electrodes ratio of 3.5 can be stably cycled over 100 cycles.
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http://dx.doi.org/10.1002/adma.202102083DOI Listing
July 2021

Elabela gene therapy promotes angiogenesis after myocardial infarction.

J Cell Mol Med 2021 Jul 21. Epub 2021 Jul 21.

Department of Cardiovascular Medicine, Affiliated Nanjing Brain Hospital, Nanjing Medical University, Nanjing, China.

This study was aimed at investigating whether Elabela (ELA) gene therapy can promote angiogenesis in the treatment of myocardial infarction (MI). The fusion expression plasmid pAAV-3 × Flag/ELA-32 was successfully constructed using molecular cloning technique. The model of acute MI was established by ligating the left anterior descending coronary artery in mice. Adeno-associated virus serotype 9 (AAV9) was injected into the surrounding myocardium and tail vein immediately after the model was established. AAV was injected again from the tail vein one week later. Compared with the MI+PBS (control) group, the serum N-terminal pro-brain natriuretic peptide (NT-proBNP) concentration, and the values of left ventricular end-diastolic diameter (LVDd) and left ventricular end-systolic diameter (LVDs) of the MI+AAV-ELA (gene therapy) group were significantly decreased, while the value of left ventricular ejection fraction was significantly increased at 2 and 4 weeks after operation. Compared with the control group, the expression of CD105 and vWF and the percentage of CD31- and Ki67-co-positive cells were significantly increased in the gene therapy group. Moreover, the expressions of apelin peptide jejunum (APJ) receptor, vascular endothelial growth factor (VEGF), VEGFR2, Jagged1 and Notch3 in the heart tissue around the infarction were up-regulated in mice with gene therapy. The results suggest that ELA activates VEFG/VEGFR2 and Jagged1/Notch3 pathways through APJ to promote angiogenesis after myocardial infarction. ELA gene therapy may be used in the treatment of ischaemic cardiomyopathy in future.
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http://dx.doi.org/10.1111/jcmm.16814DOI Listing
July 2021

Identification and expression analysis of lncRNA in seven organs of Rhinopithecus roxellana.

Funct Integr Genomics 2021 Jul 21. Epub 2021 Jul 21.

Key Laboratory of Bio-Resources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, China.

Long non-coding RNA (lncRNA) represents a new direction to identify expression profiles and regulatory mechanisms in various organisms. Here, we report the first dataset of lncRNAs of the golden snub-nosed monkey (GSM), including 12,557 putative lncRNAs identified from seven organs. Compared with mRNA, GSM lncRNA had fewer exons and isoforms, and longer length. LncRNA showed more obvious tissue-specific expression than mRNA. However, for the top ten most abundant genes in each organ, mRNAs expression was more tissue-specific than lncRNAs. By identification of specifically expressed lncRNAs and mRNAs in each organ, it indicates that the expression of SEG-lncRNA (specifically expressed lncRNA) and SEG-mRNA (specifically expressed mRNA) had high correlation. In particular, combined our lncRNA and mRNA data, we identified 92 heart SEG-lncRNAs targeted ten mRNA genes in the oxidative phosphorylation pathway and upregulated the expression of these target genes such as ND4, ATP6, and ATP8. These may contribute to GSM adaption to its high-elevation environment. We also identified 171 liver SEG-lncRNAs, which targeted 27 genes associated with the metabolism of xenobiotics and leaded to high expression of these target genes in liver. These lncRNAs may play important roles in GSM adaptation to a folivory diet.
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http://dx.doi.org/10.1007/s10142-021-00797-6DOI Listing
July 2021

Positive change in asthma control using therapeutic patient education in severe uncontrolled asthma: a one-year prospective study.

Asthma Res Pract 2021 Jul 21;7(1):10. Epub 2021 Jul 21.

Department of Allergy and Clinical Immunology, State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, 151 Yan Jiang Rd, 510000, Guangzhou, P.R. China.

Background: Severe asthma is difficult to control. Therapeutic patient education enables patients to better understand their disease and cope with treatment, but the effect of therapeutic patient education in severe uncontrolled asthma is unclear. We evaluated whether therapeutic patient education is effective in improving asthma control and decreasing the frequency of exacerbations in severe uncontrolled asthma.

Methods: This was a prospective, observational, and self-controlled study that enrolled 40 subjects with severe uncontrolled asthma. Patients were seen at a clinic four times (on day 1 and after 3, 6, and 12 months). After baseline data collection, the subjects completed a therapeutic patient education program and were also followed-up via telephone after 1, 2, 4, 5, 7, 8, 9, 10, and 11 months to monitor asthma medication adherence and collect asthma-related information.

Results: Within the 1-year study period, a total of 23 exacerbations were recorded in 14 patients, seven of whom required emergency treatment and two of whom were hospitalized. Twelve months after the standardized therapeutic patient education program, pulmonary function and fractional exhaled nitric oxide levels improved significantly in all 40 patients. Moreover, the scores from three standardized asthma questionnaires and indices suggested improved quality of life in these patients with severe uncontrolled asthma. Serum levels of biomarkers reflecting asthma immune responses did not change between baseline and the 1-year follow-up time point.

Conclusions: Therapeutic patient education is effective in improving asthma control and decreasing exacerbations in patients with severe uncontrolled asthma.
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http://dx.doi.org/10.1186/s40733-021-00076-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293484PMC
July 2021

Prognostic signature and immune efficacy of m A-, m C- and m A-related regulators in cutaneous melanoma.

J Cell Mol Med 2021 Jul 21. Epub 2021 Jul 21.

Department of Plastic Surgery of Third Xiangya Hospital, Central South University, Changsha, Hunan, China.

Cutaneous melanoma (CM) is an aggressive cancer; given that initial and specific signs are lacking, diagnosis is often late and the prognosis is poor. RNA modification has been widely studied in tumour progression. Nevertheless, little progress has been made in the signature of N -methyladenosine (m A), 5-methylcytosine (m C), N -methyladenosine (m A)-related regulators and the tumour microenvironment (TME) cell infiltration in CM. Our study identified the characteristics of m A-, m C- and m A-related regulators based on 468 CM samples from the public database. Using univariate, multivariate and LASSO Cox regression analysis, a risk model of regulators was established and validated by a nomogram on independent prognostic factors. The gene set variation analysis (GSVA) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) clarified the involved functional pathways. A combined single-sample gene set enrichment analysis (ssGSEA) and CIBERSORT approach revealed TME of regulator-related prognostic signature. The nine-gene signature stratified the patients into distinct risk subgroups for personalized prognostic assessment. Additionally, functional enrichment, immune infiltration and immunotherapy response analysis indicated that the high-risk group was correlated with T-cell suppression, while the low-risk group was more sensitive to immunotherapy. The findings presented here contribute to our understanding of the TME molecular heterogeneity in CM. Nine m A-, m C- and m A-related regulators may also be promising biomarkers for future research.
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http://dx.doi.org/10.1111/jcmm.16800DOI Listing
July 2021

Identification and Characterization of Cancer Stem-Like Cells in ALK-Positive Anaplastic Large Cell Lymphoma Using the SORE6 Reporter.

Curr Issues Mol Biol 2021 Jul 2;43(2):543-557. Epub 2021 Jul 2.

Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2E1, Canada.

Transcription factors Sox2 and Oct4 are essential in maintaining the pluripotency of embryonic stem cells and conferring stemness in cancer stem-like (CSL) cells. SORE6, an in-vitro reporter system, was designed to quantify the transcription activity of Sox2/Oct4 and identify CSL cells in non-hematologic cancers. Using SORE6, we identified and enriched CSL cells in ALK-positive anaplastic large cell lymphoma (ALK + ALCL). Two ALK + ALCL cell lines, SupM2 and UCONN-L2, contained approximately 20% of SORE6+ cells, which were purified based on their expression of green fluorescent protein. We then performed functional studies using single-cell clones derived from SORE6- and SORE6+ cells. Compared to SORE6- cells, SORE6+ cells were significantly more chemoresistant and clonogenic in colony-formation assays. Sox2/Oct4 are directly involved in conferring these CSL properties, since the shRNA knockdown of Sox2 in SORE6+ significantly lowered their chemoresistance, while enforced expression of Sox2/Oct4 in SORE6- cells produced opposite effects. Using Western blots, we found that the expression and subcellular localization of Sox2/Oct4 were similar between SORE6- and SORE6+ cells. However, in SORE6+ but not SORE6- cells, Sox2 and Oct4 abundantly bound to a probe containing the SORE6 consensus sequence. c-Myc, previously shown to regulate cancer stemness in ALK + ALCL, regulated the SORE6 activity. In conclusion, SORE6 is useful in identifying/enriching CSL cells in ALK + ALCL.
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http://dx.doi.org/10.3390/cimb43020041DOI Listing
July 2021

Repeatability of Apparent Diffusion Coefficient at 3.0 Tesla in Normal Pancreas.

Cureus 2021 Jun 18;13(6):e15734. Epub 2021 Jun 18.

Radiology, Changhai Hospital of Shanghai, Shanghai, CHN.

Purpose: To evaluate the apparent diffusion coefficient (ADC) test-retest repeatability of the normal pancreas based on diffusion-weighted imaging (DWI).

Methods: Twenty-six healthy volunteers (mean 47.6 years; 13 men) were included and scanned twice with reposition for a DWI sequence at 3.0-T. Two readers measured the ADCs of pancreatic head, body and tail for two DWIs, independently. The mean ADCs of the pancreatic head, body and tail were calculated as the global pancreatic ADC. Test-retest repeatability and agreement of ADC measurement were evaluated by the Bland-Altman analysis, intra-class correlation coefficient (ICC) and coefficient of variation (CV).

Results: The global pancreatic ADC showed the best test-retest repeatability (mean difference ± limits of agreement were 0.05 ± 0.25×10 mm/s; ICC, 0.79; CV, 6%). Test-retest repeatabilities for ADC of pancreatic head, body or tail were scattered, with mean difference ± limits of agreement between two tests were 0.03 ± 0.47, 0.05 ± 0.42 and 0.06 ± 0.31 (×10 mm/s) (ICCs, 0.81, 0.52 and 0.68; CVs, 9%, 8% and 8%), respectively. Both intra-observer repeatability and inter-observer reproducibility were acceptable for global pancreatic ADC between measurements of the two DWIs.

Conclusions: The best test-retest repeatability of ADC in the normal pancreas was only for the whole pancreas with a CV of 6%. Cautions should be taken in interpreting longitudinal clinical changes in ADC values of the normal pancreas for the measurements do have an inherent variability by locations.
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http://dx.doi.org/10.7759/cureus.15734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286541PMC
June 2021

Clinical Efficacy of Corticosteroids in the Early Stages of Deterioration in COVID-19 Pneumonia.

Infect Drug Resist 2021 12;14:2667-2674. Epub 2021 Jul 12.

Department of Respiratory Medicine, The Petroleum Clinical Medical College of Hebei Medical University, Langfang, Hebei, 065000, People's Republic of China.

Background: The World Health Organization (WHO) strongly suggests using corticosteroids in patients with severe coronavirus disease 2019 (COVID-19). Similarly, a large randomized controlled clinical trial (RCT) in the UK found that dexamethasone effectively reduced the mortality rate in severe COVID-19 patients. However, the safety profile of corticosteroids has been a controversial area of study.

Case Description: A case of a COVID-19 patient is described and the clinical characteristics are observed as the mildly symptomatic patient progresses into a critically ill patient and during their dramatic improvement with corticosteroid therapy in the early stage of the deterioration process with COVID-19 pneumonia.

Conclusion: The most suitable timing and dosage for the use of corticosteroids to maximize its effect during the worsening of COVID-19 pneumonia are discussed. One of the main pathophysiological hypotheses for severe COVID-19 patients is related to cytokine storm and virus load, which can be effectively treated with corticosteroid therapy.
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http://dx.doi.org/10.2147/IDR.S314938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285565PMC
July 2021

TGF-β1-mediated exosomal lnc-MMP2-2 increases blood-brain barrier permeability via the miRNA-1207-5p/EPB41L5 axis to promote non-small cell lung cancer brain metastasis.

Cell Death Dis 2021 Jul 20;12(8):721. Epub 2021 Jul 20.

School of Clinical Medicine, Chengdu Medical College, Chengdu, Sichuan, PR China.

Brain metastases remain a major problem in patients with advanced non-small cell lung cancer (NSCLC). The permeability of the blood-brain barrier (BBB) is highly increased during lung cancer brain metastasis; however, the underlying mechanism remains largely unknown. We previously found that lnc-MMP2-2 is highly enriched in tumor growth factor (TGF)-β1-mediated exosomes and regulates the migration of lung cancer cells. This study aimed to explore the role of exosomal lnc-MMP2-2 in the regulation of BBB and NSCLC brain metastasis. Here, using endothelial monolayers and mouse models, we found that TGF-β1-mediated NSCLC-derived exosomes efficiently destroyed tight junctions and the integrity of these natural barriers. Overexpression of lnc-MMP2-2 in human brain microvascular endothelial cells increased vascular permeability in endothelial monolayers, whereas inhibition of lnc-MMP2-2 alleviated these effects. Furthermore, lnc-MMP2-2 knockdown markedly reduced NSCLC brain metastasis in vivo. Mechanistically, through luciferase reporter assays, RNA pull-down assay, and Ago2 RNA immunoprecipitation assay, we showed that lnc-MMP2-2 served as a microRNA sponge or a competing endogenous RNA for miR-1207-5p and consequently modulated the derepression of EPB41L5. In conclusion, TGF-β1-mediated exosomal lnc-MMP2-2 increases BBB permeability to promote NSCLC brain metastasis. Thus, exosomal lnc-MMP2-2 may be a potential biomarker and therapeutic target against lung cancer brain metastasis.
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http://dx.doi.org/10.1038/s41419-021-04004-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292445PMC
July 2021

Flexible Zn-MOF with Rare Underlying Topology for Effective Separation of C6 Alkane Isomers.

ACS Appl Mater Interfaces 2021 Jul 20. Epub 2021 Jul 20.

Department of Chemistry and Chemical Biology, Rutgers University, 123 Bevier Road, Piscataway, New Jersey 08854, United States.

Adsorptive separation by porous solids provides an energy-efficient alternative for the purification of important chemical species compared to energy-intensive distillations. Particularly, the separation of linear hexane isomers from its branched counterparts is crucial to produce premium grade gasoline with high research octane number (RON). Herein, we report the synthesis of a new, flexible zinc-based metal-organic framework, [Zn(μ-OH)(adtb)(HO)·5 DMA] (Zn-adtb), constructed from a butterfly shaped carboxylate linker with underlying (4,8)-connected topology capable of separating the C6 isomers HEX, 3MP, and 23DMB. The sorbate-sorbent interactions and separation mechanisms were investigated and analyzed through in situ FTIR, solid state NMR measurements and computational modeling. These studies reveal that Zn-adtb discriminates the HEX/3MP isomer pair through a kinetic separation mechanism and the HEX/23DMB isomer pair through a molecular sieving mechanism. Column breakthrough measurements further demonstrate the efficient separation of linear HEX from the mono- and dibranched isomers.
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http://dx.doi.org/10.1021/acsami.1c08678DOI Listing
July 2021
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