Publications by authors named "Li Huang"

1,738 Publications

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Quantification of balanced SSFP myocardial perfusion imaging at 1.5 T: Impact of the reference image.

Magn Reson Med 2021 Sep 23. Epub 2021 Sep 23.

School of Biomedical Engineering and Imaging Sciences, Faculty of Life Sciences and Medicine, King's College London, London, United Kingdom.

Purpose: To investigate the use of a high flip-angle (HFA) balanced SSFP (bSSFP) reference image (in comparison to conventional proton density [PD]-weighted reference images) for conversion of bSSFP myocardial perfusion images into dynamic T maps for improved myocardial blood flow (MBF) quantification at 1.5 T.

Methods: The HFA-bSSFP (flip angle [FA] = 50°), PD gradient-echo (PD-GRE; FA = 5°), and PD-bSSFP (FA = 8°) reference images were acquired before a dual-sequence bSSFP perfusion acquisition. Simulations were used to study accuracy and precision of T and MBF quantification using the three techniques. The accuracy and precision of T , and the precision and intersegment variability of MBF were compared among the three techniques in 8 patients under rest conditions.

Results: In simulations, HFA-bSSFP demonstrated improved T /MBF precision (higher T /MBF SD of 30%-80%/50%-100% and 30%-90%/60%-115% for PD-GRE and PD-bSSFP, respectively). Proton density-GRE and PD-bSSFP were more sensitive to effective FA than HFA-bSSFP (maximum T /MBF errors of 13%/43%, 20%/43%, and 1%/3%, respectively). Sensitivity of all techniques (defined as T /MBF errors) to native T , native T , and effective saturation efficiency were negligible (<1%/<1%), moderate (<14%/<19%), and high (<63%/<94%), respectively. In vivo, no difference in T accuracy was observed among HFA-bSSFP, PD-GRE, and PD-bSSFP (-9 ± 44 ms vs -28 ± 55 ms vs -22 ± 71 ms, respectively; p > .08). The HFA-bSSFP led to improved T /MBF precision (T /MBF SD: 41 ± 19 ms/0.24 ± 0.08 mL/g/min vs PD-GRE: 48 ± 20 ms/0.29 ± 0.09 mL/g/min and PD-bSSFP: 59 ± 23 ms/0.33 ± 0.11 mL/g/min; p ≤ .02) and lower MBF intersegment variability (0.14 ± 0.09 mL/g/min vs PD-GRE: 0.21 ± 0.09 mL/g/min and PD-bSSFP: 0.20 ± 0.10 mL/g/min; p ≤ .046).

Conclusion: We have demonstrated the feasibility of using a HFA-bSSFP reference image for MBF quantification of bSSFP perfusion imaging at 1.5 T. Results from simulations demonstrate that the HFA-bSSFP reference image results in improved precision and reduced sensitivity to effective FA compared with conventional techniques using a PD reference image. Preliminary in vivo data acquired at rest also demonstrate improved precision and intersegment variability using the HFA-bSSFP technique compared with PD techniques; however, a clinical study in patients with coronary artery disease under stress conditions is required to determine the clinical significance of this finding.
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http://dx.doi.org/10.1002/mrm.29019DOI Listing
September 2021

Construction of a Fusellovirus with a Minimal Set of Genes.

ACS Synth Biol 2021 Sep 20. Epub 2021 Sep 20.

State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

A large number of fuselloviruses have been found in acidic hot springs around the globe. They share a set of highly conserved genes (core genes) and possess a varying number of less-conserved genes (non-core genes). However, the functions of most of these genes are unknown. Recent studies show that as many as half of these genes tolerate mutation. In this study, we conducted a genetic analysis on spindle-shaped virus 22 (SSV22), an alphafusellovirus with fewer open reading frames (ORFs) than most of the isolated fuselloviruses. Both deletion and frame-shift mutations were introduced into nearly all of the 26 ORFs of the viral genome. A total of 17 ORFs were indispensable, and two additional ORFs were required for the optimal infectivity of the virus. Deletion of either VP2 or VP3, the two structural proteins, did not affect the morphology or infectivity of the virus. An infectious SSV22 derivative carrying a minimal genome of 20 ORFs was obtained. The SSV22 capsid was capable of accommodating a genome as large as ∼18 kb, or ∼7 kb larger than that of the wild-type virus. The viral capsid varied in both the length and width, but not in shape, with the size of the genome. Our results will facilitate the analysis of crucial protein-protein interactions between SSV22 and the host during viral infection and help explore the use of SSV22 as a vector for DNA delivery in potential applications.
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http://dx.doi.org/10.1021/acssynbio.1c00232DOI Listing
September 2021

Secondary peripheral T-cell lymphoma and acute myeloid leukemia after Burkitt lymphoma treatment: A case report.

World J Clin Cases 2021 Aug;9(24):7237-7244

Department of Hematology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou 570311, Hainan Province, China.

Background: Multiple primary cancer refers to more than one synchronous or sequential cancer in the same individual. Multiple primary cancer always presents as solid cancer or acute myeloid leukemia (AML) secondary to lymphoma. Here, we report a rare case of secondary peripheral T-cell lymphoma and AML after Burkitt lymphoma treatment.

Case Summary: A 54-year-old female patient was admitted to our hospital complaining of edema on her left lower limb. Physical examination revealed multiple superficial lymphadenectasis on her neck and pelvis. Color ultrasonography examination showed multiple uterine fibroids and a solid mass at the lower left side of the abdomen. Pathological biopsy revealed Burkitt lymphoma. After three hyper-CVAD (A + B) regimens, she achieved complete remission. Two years later, lymphadenectasis reoccurred. A relevant biopsy confirmed the diagnosis of peripheral T-cell lymphoma, which was accompanied by gastrointestinal invasion and hemocytopenia. Meanwhile, bone marrow examination revealed AML. On the second day of scheduled treatment, she developed gastrointestinal bleeding, peptic ulcers, and hemorrhagic shock and was critically ill. She was then discharged from the hospital due to financial concerns.

Conclusion: This is the first report of secondary peripheral T-cell lymphoma and AML after Burkitt lymphoma treatment with heterochronous and synchronal multiple primary cancers.
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http://dx.doi.org/10.12998/wjcc.v9.i24.7237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8409196PMC
August 2021

High-frequency and activation of CD4CD25 T cells maintain persistent immunotolerance induced by congenital ALV-J infection.

Vet Res 2021 Sep 15;52(1):119. Epub 2021 Sep 15.

College of Veterinary Medicine, Shandong Agricultural University, No 61, Daizong Street, Tai'an, 271018, Shandong, China.

Congenital avian leukosis virus subgroup J (ALV-J) infection can induce persistent immunotolerance in chicken, however, the underlying mechanism remains unclear. Here, we demonstrate that congenital ALV-J infection induces the production of high-frequency and activated CD4CD25 Tregs that maintain persistent immunotolerance. A model of congenital infection by ALV-J was established in fertilized eggs, and hatched chicks showed persistent immunotolerance characterized by persistent viremia, immune organ dysplasia, severe imbalance of the ratio of CD4/CD8 T cells in blood and immune organs, and significant decrease in CD3 T cells and Bu-1 B cells in the spleen. Concurrently, the mRNA levels of IL-2, IL-10, and IFN-γ showed significant fluctuations in immune organs. Moreover, the frequency of CD4CD25 Tregs in blood and immune organs significantly increased, and the frequency of CD4CD25 Tregs was positively correlated with changes in ALV-J load in immune organs. Interestingly, CD4CD25 Tregs increased in the marginal zone of splenic nodules in ALV-J-infected chickens and dispersed to the germinal center. In addition, the proliferation and activation of B cells in splenic nodules was inhibited, and the number of IgM and IgG cells in the marginal zone significantly decreased. We further found that the mRNA levels of TGF- β and CTLA-4 in CD4CD25 Tregs of ALV-J-infected chickens significantly increased. Together, high-frequency and activated CD4CD25 Tregs inhibited B cells functions by expressing the inhibitory cytokine TGF-β and inhibitory surface receptor CTLA-4, thereby maintaining persistent immunotolerance in congenital ALV-J-infected chickens.
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http://dx.doi.org/10.1186/s13567-021-00989-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8442411PMC
September 2021

Gene body methylation safeguards ribosomal DNA transcription by preventing PHF6-mediated enrichment of repressive histone mark H4K20me3.

J Biol Chem 2021 Sep 11:101195. Epub 2021 Sep 11.

The MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University, Beijing 100871, China. Electronic address:

DNA methylation shows complex correlations with gene expression, and the role of promoter hypermethylation in repressing gene transcription has been well addressed. Emerging evidence indicates that gene body methylation promotes transcription; however, the underlying mechanisms remain to be further investigated. Here, using methylated DNA immunoprecipitation sequencing (MeDIP-seq), bisulfite genomic sequencing and immunofluorescent labeling, we show that gene body methylation is indeed positively correlated with rRNA gene (rDNA) transcription. Mechanistically, gene body methylation is largely maintained by DNA methyltransferase 1 (DNMT1), deficiency or downregulation of which during myoblast differentiation or nutrient deprivation results in decreased gene body methylation levels, leading to increased gene body occupancy of plant homeodomain (PHD) finger protein 6 (PHF6). PHF6 binds to hypomethylated rDNA gene bodies where it recruits histone methyltransferase SUV4-20H2 to establish the repressive histone modification, H4K20me3, ultimately inhibiting rDNA transcription. These findings demonstrate that DNMT1-mediated gene body methylation safeguards rDNA transcription by preventing enrichment of repressive histone modifications, suggesting that gene body methylation serves to maintain gene expression in response to developmental and/or environmental stresses.
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http://dx.doi.org/10.1016/j.jbc.2021.101195DOI Listing
September 2021

NBN-Doped Bis-Tetracene and Peri-Tetracene: Synthesis and Characterization.

Angew Chem Int Ed Engl 2021 Sep 14. Epub 2021 Sep 14.

Technische Universitaet Dresden, Chair for Molecular Functional Materials, Mommsenstrasse 4, 01062, Dresden, GERMANY.

Combining solution-based and surface-assisted synthesis, we demonstrate here the first synthesis of NBN-doped bis -tetracene ( NBN-BT ) and peri -tetracene ( NBN-PT ). Unlike unstable all-carbon-based bis -tetracene and peri -tetracene, both NBN-BT and NBN-PT exhibit excellent stability under ambient conditions. The chemical structures of NBN-BT and NBN-PT are clearly elucidated by high-resolution scanning tunneling microscopy (STM) in combination with noncontact atomic force microscopy (nc-AFM). Scanning tunneling spectroscopy (STS) characterizations reveal that NBN-BT (3.08 eV) and NBN-PT ( 3.28 eV) possess higher energy gaps than their carbon-based bis -tetracene (1.56 eV) and peri -tetracene (1.11 eV) analogs. Interestingly, based on its in situ spectroelectrochemical (SEC) characterization, NBN-BT can undergo stepwise one-electron oxidation and convert into its corresponding radical cation first and then to its dication rather than its diradical dication. The energy gap of the NBN-BT dication (1.24 eV) is similar to that of bis -tetracene (1.56 eV), indicating their isoelectronic relationship. Moreover, a similar energy gap between the NBN-PT dication (1.21 eV) and its isoelectronic structure peri -tetracene (1.11 eV) can be predicted by DFT calculations. This work provides a novel synthesis along with characterizations of multi-NBN-doped zigzag-edged peri -acenes with tunable electronic properties that are not accessible with classical all-carbon-based peri -acenes.
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http://dx.doi.org/10.1002/anie.202109808DOI Listing
September 2021

Dihydromyricetin attenuates palmitic acid-induced oxidative stress by promoting autophagy via SIRT3-ATG4B signaling in hepatocytes.

Nutr Metab (Lond) 2021 Sep 9;18(1):83. Epub 2021 Sep 9.

Research Center for Nutrition and Food Safety, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing Medical Nutrition Research Center, Institute of Military Preventive Medicine, Third Military Medical University, 30th Gaotanyan Main Street, Shapingba District, 400038, Chongqing, People's Republic of China.

Background: Oxidative stress in hepatocytes was important pathogenesis of nonalcoholic steatohepatitis (NASH). Autophagy was a cellular process that can remove damaged organelles under oxidative stress, and thus presented a potential therapeutic target against NASH. This work aimed to investigate whether autophagy was participated in the protective effects of dihydromyricetin (DHM) on palmitic acid (PA)-induced oxidative stress in hepatocytes and the underlying mechanism.

Methods: HepG2 and HHL-5 cell lines were pretreated with DHM (20 μM) for 2 h, followed by PA (0.2 mM) treatment for 16 h. The oxidative stress was assessed by the quantification of intracellular reactive oxygen species (ROS), mitochondrial ROS (mtROS), mitochondrial membrane potential (MMP) and mitochondrial ultrastructural analyses. The protein expressions of SIRT3, LC3I/II, P62 and ATG4B, as well as the acetylation of AGT4B were determined by western blotting using HepG2 and HepG2/ATG4B cells with heterozygous knockout of ATG4B.

Results: Exposure to PA resulted in increased intracellular ROS and mtROS, decreased MMP and aggravated mitochondrial injury in HepG2 cells, which were notably attenuated by DHM treatment. DHM-induced inhibition of oxidative stress was associated with the induction of autophagy, characterized by upregulated ATG4B and LC3 II as well as downregulated P62 levels. Furthermore, the inhibitory effects of DHM on PA-induced autophagy arrest and oxidative stress were eliminated when pretreated with a SIRT3 inhibitor 3-TYP or conducted in HepG2/ATG4B cells, suggesting that SIRT3 and ATG4B were involved in DHM-induced benefits. Moreover, DHM treatment increased the protein expression of SIRT3 and SIRT3-dependent deacetylation of ATG4B in HepG2 cells.

Conclusion: Our results demonstrated that DHM attenuated PA-induced oxidative stress in hepatocytes through induction of autophagy, which was mediated through the increased expression of SIRT3 and SIRT3-mediated ATG4B deacetylation following DHM treatment.
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http://dx.doi.org/10.1186/s12986-021-00612-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428134PMC
September 2021

Comparisons of different exercise interventions on glycemic control and insulin resistance in prediabetes: a network meta-analysis.

BMC Endocr Disord 2021 Sep 6;21(1):181. Epub 2021 Sep 6.

Institute of Physical Education, Shanghai Normal University, No. 5, Lane 14, Guilin West Street, Xuhui District, Shanghai, 200234, China.

Background: In order to recommend the optimal type of exercise for type 2 diabetes prevention, different exercise interventions were compared with respect to their effects on glycemic control and insulin resistance.

Methods: Studies on the curative effect of aerobic exercise training (AET), resistance training (RT), or control training (CT) on prediabetes were retrieved from the PubMed, Embase, SPORTDiscus, and Cochrane Library databases. Body mass index (BMI); homeostasis model assessment of insulin resistance index (HOMA-IR); and fasting blood glucose (FBG), glycated hemoglobin (HbA1c), and insulin levels were used as outcome indicators. The Q statistic was calculated to evaluate heterogeneity within studies. A fixed- or random-effects model was used for pooling data based on nonsignificant or significant heterogeneities. A consistency test was conducted using a node-splitting analysis.

Results: A total of 13 eligible studies were included. The results of the direct meta-analysis indicated that AET or RT could significantly reduce the HbA1c level in prediabetic individuals compared to CT [AET vs. CT: standardized mean difference (SMD) = - 0.6739, 95% confidence interval (CI) = - 0.9424 to - 0.4055 to RT vs. CT: SMD = - 1.0014, 95% CI = - 1.3582 to - 0.6446]. The findings from the network meta-analysis showed that there were no statistical differences among the four comparisons for all the indicators except for lower HbA1c level (SMD = - 0.75, 95% CI = - 1.31 to - 0.19) and HOMA-IR (SMD = - 1.03, 95% CI = - 1.96 to - 0.10) in the AET group than in the CT group. In addition, prediabetic individuals in the AET + RT group showed greater control of BMI and insulin and FBG levels than those in the other groups, whereas AET was the most effective in controlling HbA1c and HOMA-IR levels in prediabetic individuals.

Conclusion: AET, AET + RT, and RT exerted beneficial effects on insulin resistance and glycemic control in prediabetic patients. From the existing data, AET or AET + RT is preferentially recommended for these patients, although further studies may unveil RT as a promising therapy. Benefits from all types of exercise seem to occur in an intensity-dependent manner.
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http://dx.doi.org/10.1186/s12902-021-00846-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422751PMC
September 2021

Effects of increasing serum luteinizing hormone levels during early phase of the gonadotropin-releasing hormone antagonist protocol on clinical outcomes of the fertilization cycle.

Gynecol Endocrinol 2021 Sep 6:1-5. Epub 2021 Sep 6.

Department of Reproductive Health and Infertility, Guangdong Women and Children Hospital, Guangzhou, China.

Objective: To determine the effects of changes in serum luteinizing hormone (LH) levels in the early stages of the gonadotropin-releasing hormone antagonist (GnRH-A) protocol on fertilization and embryo transfer/intracytoplasmic sperm injection clinical outcomes.

Methods: Data from 2116 fresh embryo transfer cycles with the GnRH-A protocol were retrospectively analyzed. Patients were divided into two groups, ΔLH-increased and ΔLH-decreased, according to changes in serum LH levels on the day of GnRH-A addition compared with that on the start day of ovarian stimulation. Patients in whom ΔLH increased were categorized according to early-onset LH increases (serum LH level ≥10 mIU/mL or twice the baseline).

Results: ΔLH increased and decreased in 14.9% and 85.1% of patients, respectively. The fertilization rate was lower, and fewer oocytes were retrieved in patients with increased ΔLH compared to those with decreased ΔLH ( .05). The number of AFC, oocytes retrieved, and AMH in patients with early-onset ΔLH increase was lower between the subgroups ( < .05). There were no significant differences in clinical pregnancy, early abortion, biochemical pregnancy, and live birth rates between the groups and subgroups ( > .05).

Conclusions: Early increases in LH levels during GnRH-A protocol might affect the number of oocytes retrieved, but not the clinical outcomes.
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http://dx.doi.org/10.1080/09513590.2021.1955341DOI Listing
September 2021

Effects of Andrographolide on Mouse Intestinal Microflora Based on High-Throughput Sequence Analysis.

Front Vet Sci 2021 18;8:702885. Epub 2021 Aug 18.

College of Animal Science, Wenzhou Vocational College of Science and Technology, Wenzhou, China.

The intestinal flora is a micro-ecosystem that is closely linked to the overall health of the host. We examined the diversity and abundance of intestinal microorganisms in mice following the administration of andrographolide, a component of the Chinese medical herb . Administration of andrographolide produces multiple beneficial effects including anti-inflammatory, antiviral and antibacterial effects but whether it directly influences the gut microbiota is not known. This study investigated whether the oral administration of andrographolide influences the intestinal microbiota and was compared with amoxicillin treatment as a positive control and water only as a negative control. We examined 21 cecal samples and conducted a high-throughput sequencing analysis based on V3-V4 variable region of the 16S rDNA genes. We found that the diversity and abundance of mouse gut microbiota decreased in direct proportion with the amoxicillin dose whereas andrographolide administration did not affect intestinal microbial community structure. The composition of intestinal microbes following andrographolide treatment was dominated by the while dominated the amoxicillin treatment group compared with the negative controls. Specifically, the and were enriched with andrographolide administration while and significantly increased in the amoxicillin test groups. Amoxicillin administration altered the microbial community composition and structure by increasing the proportion of pathogenic to beneficial bacteria whereas andrographolide administration led to increases in the proportions and abundance of beneficial bacteria. This study provides a theoretical basis for finding alternatives to antibiotics to decrease bacterial resistance and restore intestinal floral imbalances.
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http://dx.doi.org/10.3389/fvets.2021.702885DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416444PMC
August 2021

Efficient generation of narrowband picosecond pulses from a femtosecond laser.

Rev Sci Instrum 2021 Aug;92(8):083001

State Key Laboratory of Molecular Reaction Dynamics and Dynamics Research Center for Energy and Environmental Materials, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

In some applications of broadband ultrafast spectroscopy, such as surface sum frequency generation vibrational spectroscopy, femtosecond stimulated Raman spectroscopy (SRS), and coherent anti-Stokes Raman spectroscopy, a narrowband picosecond pulse is required to obtain a high spectral resolution. Here, we present a method to generate narrowband picosecond second harmonic (SH) and fundamental frequency (FF) pulses with high-conversion efficiency from a Ti:sapphire femtosecond laser amplifier. The narrowband picosecond SH pulse was generated based on the group velocity mismatch between the SH and FF pulses in a nonlinear crystal of β-barium borate (BBO). The small SH nonlinear optical coefficient was optimized by changing the azimuth angle of a thick BBO crystal, successfully avoiding the saturation effect in the SH generation process. The SH pulse was then used to pump an optical parametric amplifier to efficiently amplify the narrowband FF seed pulse, which was obtained with an etalon by spectrally filtering the output from the femtosecond laser amplifier. Dual-wavelength output, which could be very useful in femtosecond SRS, was also realized.
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http://dx.doi.org/10.1063/5.0056050DOI Listing
August 2021

Ezrin regulates synovial angiogenesis in rheumatoid arthritis through YAP and Akt signalling.

J Cell Mol Med 2021 Aug 29. Epub 2021 Aug 29.

Department of Joint and Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

This study aimed to investigate the role and regulatory mechanisms of Ezrin in synovial vessels in rheumatoid arthritis (RA). Synovial tissues were obtained from people with osteoarthritis people and patients with RA patients. We also used an antigen-induced arthritis (AIA) mice model by using Freund's adjuvant injections. Ezrin expression was analysed by immunofluorescence and immunohistochemical staining in synovial vessels of patients with RA and AIA mice. We investigated the role of Ezrin on vascular endothelial cells and its regulatory mechanism in vivo and in vitro by adenoviral transfection technology. Our results suggest a role for the Ezrin protein in proliferation, migration and angiogenesis of vascular endothelial cells in RA. We also demonstrate that Ezrin plays an important role in vascular endothelial cell migration and tube formation through regulation of the Hippo-yes-associated protein 1 (YAP) pathway. YAP, as a key protein, can further regulate the activity of PI3K/Akt signalling pathway in vascular endothelial cells. In AIA mice experiments, we observed that the inhibition of Ezrin or of its downstream YAP pathway can affect synovial angiogenesis and may lead to progression of RA. In conclusion, Ezrin plays an important role in angiogenesis in the RA synovium by regulating YAP nuclear translocation and interacting with the PI3K/Akt signalling pathway.
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http://dx.doi.org/10.1111/jcmm.16877DOI Listing
August 2021

Protective effects of intravitreal administration of mesenchymal stem cell-derived exosomes in an experimental model of optic nerve injury.

Exp Cell Res 2021 Oct 27;407(1):112792. Epub 2021 Aug 27.

Department of Ophthalmology, Fujian Provincial Hospital, Fuzhou City, Fujian Province, 350001, China. Electronic address:

Traumatic optic neuropathy results in the loss of retinal ganglion cells (RGCs), leading to unavoidable visual impairment. However, there is no effective therapy by far. Accumulated studies support the perception that mesenchymal stem cells (MSCs) secrete exosomes that serve as a protective paracrine factor. The study aimed to explore and evaluate the potential therapeutic effects of intravitreal transplantation of MSC-derived exosomes (MSC-exos) in an experimental model of optic nerve crush (ONC). Exosomes were isolated from rat MSCs and characterized by transmission electron microscope and western blotting. At the onset of ONC, a single intravitreal injection of exosomes or PBS was administered to the rats. At day 30, hematoxylin and eosin staining, immunohistochemistry, and βIII-tubulin staining were performed to evaluate the survival of RGCs. Moreover, TUNEL assay was used to examine the apoptosis of RGCs. Inflammation-relevant factors were identified via quantitative polymerase chain reaction. The expression levels of cell apoptosis-related molecules and key members of the PI3K/AKT signaling pathway were determined via western blot analysis. We found that MSC-exos exhibited typical characteristic morphologies (cup-shaped) and sizes (peak size of 93 nm). Furthermore, they exhibited substantial expression of the exosome markers CD63 and TSG101, but lacked the expression of the cellular marker GM130. Treatment with intravitreal MSC-exos notably promoted the survival of RGCs in ONC rats. The level of pro-inflammatory cytokines, including TNF-α, IL-1β, IL-6, IL-8, and MCP-1, were reduced, whereas those of the anti-inflammatory factor IL-10 were increased. Moreover, the apoptosis induced by ONC was decreased by the administration of MSC-exos via upregulation of the Bcl-2/Bax ratio and downregulation of caspase-3 activity. Furthermore, MSC-exos significantly stimulated AKT phosphorylation, whereas LY294002 restored the apoptosis-preventing effects of MSC-exos. The results of our results demonstrated that intravitreal administration of MSC-exos ameliorates ONC-induced injury in a rat model. These findings might aid in the development of effective exosome-based therapeutic strategies for the treatment of optic nerve degeneration.
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http://dx.doi.org/10.1016/j.yexcr.2021.112792DOI Listing
October 2021

The activation and limitation of the bacterial natural transformation system: The function in genome evolution and stability.

Microbiol Res 2021 Aug 24;252:126856. Epub 2021 Aug 24.

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China; Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, 611130, PR China. Electronic address:

Bacteria can take up exogenous naked DNA and integrate it into their genomes, which has been regarded as a main contributor to bacterial evolution. The competent status of bacteria is influenced by environmental cues and by the immune systems of bacteria. Here, we review recent advances in understanding the working mechanisms underlying activation of the natural transformation system and limitations thereof. Environmental stresses including the presence of antimicrobials can activate the natural transformation system. However, bacterial enzymes (nucleases), non-coding RNAs, specific DNA sequences, the restriction-modification (R-M) systems, CRISPR-Cas systems and prokaryotic Argonaute proteins (Agos) are have been found to be involved in the limitation of the natural transformation system. Together, this review represents an opportunity to gain insight into bacterial genome stability and evolution.
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http://dx.doi.org/10.1016/j.micres.2021.126856DOI Listing
August 2021

Association between endogenous LH level prior to progesterone administration and live birth rate in artificial frozen-thawed blastocyst transfer cycles of ovulatory women.

Hum Reprod 2021 Sep;36(10):2687-2696

Center for Reproductive Medicine, Guangdong Women and Children Hospital, Guangzhou, Guangdong Province, China.

Study Question: Is there an association between serum LH levels prior to progesterone administration and live birth rate (LBR) in artificial frozen-thawed embryo transfer (FET) cycles?

Summary Answer: : Low serum LH levels on the day before progesterone initiation in artificial frozen-thawed blastocyst transfer cycles of ovulatory women are associated with a lower LBR.

What Is Known Already: In artificial FET cycles, exogenous oestrogen and progesterone are administered sequentially to mimic the serum hormone pattern similar to the natural cycle. In oestrogen-only phase, the supplemental oestrogen causes thickening of the endometrium and is sometimes accompanied by a rise in serum LH. However, whether the endogenous LH level in artificial FET cycles is related to clinical outcomes remains unclear.

Study Design, Size, Duration: A retrospective cohort study including 3469 artificial frozen-thawed blastocyst transfer cycles was conducted at a tertiary-care academic medical centre between February 2014 and January 2020.

Participants/materials, Setting, Methods: A total of 3469 frozen blastocyst transfer cycles were stratified into four groups based on the quartiles of serum LH level before progesterone initiation: <25th percentile (LH < 8.79 mIU/ml), 25-50th percentile (8.79 ≤ LH ≤ 13.91 mIU/ml), 51-75th percentile (13.91 < LH ≤ 20.75 mIU/ml) and >75th percentile (LH > 20.75 mIU/ml). The serum LH level >75th percentile group was considered as the reference group. Patients with polycystic ovarian syndrome or other ovulatory disorders were excluded from the study. We also excluded cycles with an endometrial thickness <7 mm before progesterone initiation and patients with intrauterine adhesions and uterine abnormalities. In order to avoid the interference of BMI, all patients were divided into two categories based on the overweight threshold: BMI <25 kg/m2 and ≥25 kg/m2, and the impacts of serum LH levels on LBR were investigated separately. Univariable and multivariable logistic regression analysis were performed to adjust for potential confounders. EmpowerStats software and R-project were used to build smooth curve fitting models.

Main Results And The Role Of Chance: Compared with the reference group, the implantation rate significantly decreased with low LH levels (<25th percentile) on the day before progesterone initiation (odds ratio [OR] = 0.74; 95% CI, 0.64-0.86; P = 0.001). Accounting for major covariates, low LH levels were associated with a relatively lower LBR (adjusted OR = 0.649; 95% CI, 0.531-0.794; P < 0.001), mainly due to a lower implantation rate, lower clinical pregnancy rate and higher pregnancy loss rate. Moreover, in the patients with BMI <25 kg/m2, low LH was associated with a lower LBR (P < 0.001); while in the overweight subgroup, LBR and LH were not correlated (P = 0.823).

Limitations, Reasons For Caution: The main limitation of this study is its retrospective design. Owing to the relatively small number in the overweight group, the results of the overweight subgroup should be interpreted with caution.

Wider Implications Of The Findings: The evidence provided in this study shows the importance of serum LH levels on the day before progesterone initiation in patients undergoing artificial FET cycles. Hypothalamic dysfunction may be one of the important causes of a relatively low LH, which is related to impaired pregnancy outcomes. Serum LH levels may be used as one of the clinical indicators to predict pregnancy outcomes.

Study Funding/competing Interest(s): No funding and no competing interest were involved in this study.

Trial Registration Number: NA.
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http://dx.doi.org/10.1093/humrep/deab172DOI Listing
September 2021

Development and validation of a novel survival model for acute myeloid leukemia based on autophagy-related genes.

PeerJ 2021 12;9:e11968. Epub 2021 Aug 12.

Department of Hematology, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University), Haikou, China.

Background: Acute myeloid leukemia (AML) is one of the most common blood cancers, and is characterized by impaired hematopoietic function and bone marrow (BM) failure. Under normal circumstances, autophagy may suppress tumorigenesis, however under the stressful conditions of late stage tumor growth autophagy actually protects tumor cells, so inhibiting autophagy in these cases also inhibits tumor growth and promotes tumor cell death.

Methods: AML gene expression profile data and corresponding clinical data were obtained from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, from which prognostic-related genes were screened to construct a risk score model through LASSO and univariate and multivariate Cox analyses. Then the model was verified in the TCGA cohort and GEO cohorts. In addition, we also analyzed the relationship between autophagy genes and immune infiltrating cells and therapeutic drugs.

Results: We built a model containing 10 autophagy-related genes to predict the survival of AML patients by dividing them into high- or low-risk subgroups. The high-risk subgroup was prone to a poorer prognosis in both the training TCGA-LAML cohort and the validation GSE37642 cohort. Univariate and multivariate Cox analysis revealed that the risk score of the autophagy model can be used as an independent prognostic factor. The high-risk subgroup had not only higher fractions of CD4 naïve T cell, NK cell activated, and resting mast cells but also higher expression of immune checkpoint genes and . Last, we screened drug sensitivity between high- and low-risk subgroups.

Conclusion: The risk score model based on 10 autophagy-related genes can serve as an effective prognostic predictor for AML patients and may guide for patient stratification for immunotherapies and drugs.
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http://dx.doi.org/10.7717/peerj.11968DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364747PMC
August 2021

Effect of Nutritional Determinants and TonB on the Natural Transformation of .

Front Microbiol 2021 10;12:644868. Epub 2021 Aug 10.

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

is a gram-negative bacterium that is the first naturally competent bacterium identified in the family . However, the determinants that influence the natural transformation and the underlying mechanism remain unknown. In this study, we evaluated the effects of various nutritional factors of the GCB medium [glucose, L-glutamine, vitamin B1, Fe (NO), NaCl, phosphate, and peptone], on the natural transformation of ATCC 11845. Among the assayed nutrients, peptone and phosphate affected the natural transformation of ATCC 11845, and the transformation frequency was significantly decreased when phosphate or peptone was removed from the GCB medium. When the iron chelator 2,2'-dipyridyl (Dip) was added, the transformation frequency was decreased by approximately 100-fold and restored gradually when Fe (NO) was added, suggesting that the natural transformation of ATCC 11845 requires iron. Given the importance of TonB in nutrient transportation, we further identified whether TonB is involved in the natural transformation of ATCC 11845. Mutation of or , but not , was shown to inhibit the natural transformation of ATCC 11845 in the GCB medium. In parallel, it was shown that the mutant, but not the mutant, decreased iron acquisition in the GCB medium. This result suggested that the mutant affects the natural transformation frequency due to the deficiency of iron utilization.
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http://dx.doi.org/10.3389/fmicb.2021.644868DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8383284PMC
August 2021

Convalescent plasma is of limited clinical benefit in critically ill patients with coronavirus disease-2019: a cohort study.

J Transl Med 2021 08 26;19(1):365. Epub 2021 Aug 26.

The Joint Center for Infection and Immunity, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou 510623, China; Institute Pasteur of Shanghai, Chinese Academy of Science, Shanghai, 200031, China.

Background: Recently, convalescent plasma (CP) transfusion was employed for severe or critically ill patients with coronavirus disease-2019. However, the benefits of CP for patients with different conditions are still in debate. To contribute clinical evidence of CP on critically ill patients, we analyze the characteristics and outcomes of patients with or without CP transfusion.

Methods: In this cohort study, 14 patients received CP transfusion based on the standard treatments, whereas the other 10 patients received standard treatments as control. Clinical characteristics and outcomes were analyzed. The cumulative survival rate was calculated by Kaplan-Meier survival analysis.

Results: Data analysis was performed on 24 patients (male/female: 15/9) with a median age of 64.0 (44.5-74.5) years. Transient fever was reported in one patient. The cumulative mortality was 21% (3/14) in patients receiving CP transfusion during a 28-day observation, whereas one dead case (1/10) was reported in the control group. No significant difference was detected between groups in 28-day mortality (P = 0.615) and radiological alleviation of lung lesions (P = 0.085).

Conclusion: In our current study, CP transfusion was clinically safe based on the safety profile; however, the clinical benefit was not significant in critically ill patients with more comorbidities at the late stage of disease during a 28-day observation.
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http://dx.doi.org/10.1186/s12967-021-03028-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390032PMC
August 2021

LC-MS Identification, Isolation, and Structural Elucidation of Anti-HIV Tigliane Diterpenoids from .

J Nat Prod 2021 08 17;84(8):2366-2373. Epub 2021 Aug 17.

Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan.

Structurally diverse tigliane diterpenoids have drawn significant research interest for drug discovery over many decades. Using LC-MS-guided fractionation and separation, the first phytochemical investigation on led to the isolation of eight tiglianes (-), including two new compounds, wikstrocin D () and wikstrocin E (). The new structures were elucidated based on extensive physicochemical and spectroscopic analyses. The characteristic ESIMS/MS fragmentations of tiglianes - were also summarized. Among the isolated tiglianes, three compounds (, , and ) showed the most potent anti-HIV activity, with IC values of 0.18, 3.8, and 12.8 nM, respectively.
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http://dx.doi.org/10.1021/acs.jnatprod.1c00570DOI Listing
August 2021

LOXL2 attenuates osteoarthritis through inactivating Integrin/FAK signaling.

Sci Rep 2021 Aug 23;11(1):17020. Epub 2021 Aug 23.

Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.

Temporomandibular joint OA (TMJOA) is a common degenerative joint disease, leads to structural damage and ultimately loss of function. Matrix degradation is one of the first pathogenesis during the progression of OA, it was effective to inhibit matrix degradation to block the development of OA. In this study, an in vivo model (compressive mechanical force) and an in vitro model (IL-1β) were used to induce OA-like changes in TMJ cartilage and chondrocytes. We revealed lysyl oxidase like-2 (LOXL2) play a critical role in TMJOA. LOXL2 expression decreased in mechanical stress/IL-β induced TMJOA-like lesions in both in vivo models and in vitro models. Furthermore, recombinant LOXL2 (rhLOXL2) treatment ameliorated the degenerative changes induced by mechanical stress in vivo, including the thinning cartilage, down-expression of collagen II and proteoglycan, and over-expression of TNF-a, while LOXL2 antibody (anti-LOXL2) treatment exacerbated these changes. Mechanistically, the protection of LOXL2 in chondrocytes was induced partly through activation of the Integrin/FAK pathway. The inhibition of the Integrin/FAK pathway could neutralized the effects caused by rhLOXL2. Collectively, our study suggests that the LOXL2 plays a protective role in mechanical stress induced TMJOA-like changes, and the Integrin/FAK pathway may be a key downstream pathway in this process.
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http://dx.doi.org/10.1038/s41598-021-96348-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382747PMC
August 2021

Abnormalities of Brain White Matter in Type 2 Diabetes Mellitus: A Meta-Analysis of Diffusion Tensor Imaging.

Front Aging Neurosci 2021 6;13:693890. Epub 2021 Aug 6.

National-Local Joint Engineering Research Center of Rehabilitation Medicine Technology, Fujian University of Traditional Chinese Medicine, Fuzhou, China.

The study aimed to conduct a meta-analysis to determine the abnormalities of white matter in patients with type 2 diabetes mellitus (T2DM) by identifying the consistency of diffusion tensor imaging (DTI). The literature for DTI comparing patients with T2DM with controls published before October 30, 2020, were reviewed in PubMed, Web of Science, Embase, CNKI, and Wan Fang databases. The meta-analysis was performed using the activation likelihood estimation (ALE) method, including 12 reports and 381 patients with T2DM. The meta-analysis identified 10 white matter regions that showed a consistent reduction of fractional anisotropy (FA) in patients with T2DM, including genu of the corpus callosum, the body of corpus callosum, bilateral anterior corona radiata, bilateral superior corona radiata, bilateral cingulum, and bilateral superior fronto-occipital fasciculus. This study revealed the abnormal characteristics of white matter in T2DM, which would be helpful to understand the underlying neuropathological and physiological mechanisms of T2DM and provide evidence for clinical diagnosis and treatment.
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http://dx.doi.org/10.3389/fnagi.2021.693890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8378805PMC
August 2021

Bacterial Viruses Subcommittee and Archaeal Viruses Subcommittee of the ICTV: update of taxonomy changes in 2021.

Arch Virol 2021 Aug 21. Epub 2021 Aug 21.

Latvian Biomedical Research and Study Center, Riga, Latvia.

In this article, we - the Bacterial Viruses Subcommittee and the Archaeal Viruses Subcommittee of the International Committee on Taxonomy of Viruses (ICTV) - summarise the results of our activities for the period March 2020 - March 2021. We report the division of the former Bacterial and Archaeal Viruses Subcommittee in two separate Subcommittees, welcome new members, a new Subcommittee Chair and Vice Chair, and give an overview of the new taxa that were proposed in 2020, approved by the Executive Committee and ratified by vote in 2021. In particular, a new realm, three orders, 15 families, 31 subfamilies, 734 genera and 1845 species were newly created or redefined (moved/promoted).
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http://dx.doi.org/10.1007/s00705-021-05205-9DOI Listing
August 2021

Review of the toxic effect of microplastics on terrestrial and aquatic plants.

Sci Total Environ 2021 Oct 7;791:148333. Epub 2021 Jun 7.

Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture, College of Natural Resources and Environment, Northwest A & F University, Yangling 712100, China. Electronic address:

As a new pollutant, microplastics (MPs) exhibited more and more significant influence on the organisms in the environment. Especially, the effects on the growth and development of plants by MPs attracted wide attentions in recent period. In the review article, we summarized the important influences of MPs on terrestrial and aquatic plants. The properties, including type, size and oxygen-containing group of MPs on their physical injures toward plants were critically reviewed, which were significantly correlated to the toxicity to plants. The secondary risks of MPs including the additives and MP-derived organic products and the adsorbed environmental pollutants to plants were clearly revealed. The hydrophobic organic pollutants released from MPs showed significant chemical effects on the plants. We also outlined the effects of MPs to the various regions (e.g. the seed and rhizome) of plants and compared the toxic difference of MPs on terrestrial and aquatic plants. Generally, the seed and rhizome of plants were susceptible to MPs, and the effects of MPs on terrestrial and aquatic plants were different. The review paper improves the understanding of potential toxicity of MP themselves and the released and adsorbed chemicals to plants in the environment.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148333DOI Listing
October 2021

Psychological Effects of People Isolated in Hubei Due to COVID-19 Epidemic.

Front Psychiatry 2021 28;12:597894. Epub 2021 Jul 28.

Medical Imaging Center, First Affiliated Hospital of Jinan University, Guangzhou, China.

The Coronavirus Disease 2019 (COVID-19) epidemic broke out from Wuhan in Hubei province, China, spread nationwide and then gradually developed into other countries in the world. The implementation of unprecedented strict isolation measures has affected many aspects of people's lives and posed a challenge to psychological health. To explore whether people isolated for 14 days due to having contact with COVID-19 patients had more psychosocial problems. We conducted an online survey from February 29 to March 10, 2020. Depression, anxiety, post-traumatic stress disorder (PTSD) symptoms, and coping style were assessed by the Patient Health Questionnaire-9 (PHQ-9), 7-item Generalized Anxiety Disorder Scale (GAD-7), Impact of Event Scale-Revised (IES-R), and Simplified Coping Style Questionnaire-20-Chinese Version. This study included 1,315 isolated respondents in Hubei province (58.5% located in Wuhan). 69.3% respondents isolated at home, 30.7% respondents isolated at centralized quarantined spot. Of all respondents, 66.8% reported depressive symptoms, 49.7% reported anxiety symptoms, 89.0% reported PTSD symptoms. The Cronbach α of the IES-R, PHQ-9, GAD-7, and total SCSQ-20 were 0.935, 0.847, 0.843, and 0.888, respectively. Persons who isolated at home were associated with a lower risk of PTSD, depressive and anxiety symptoms ( < 0.01). People who knew someone to have COVID-19 were associated with severe symptoms of PTSD symptoms ( = 0.001). As for coping style, higher level of passive coping style was associated with severe symptoms of PTSD, depression and anxiety ( < 0.001). Our findings identify that person isolated during the COVID-19 epidemic was associated with high proportion of depression, anxiety, and PTSD symptoms. Public health officials should be aware of and prepared to take necessary measures.
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http://dx.doi.org/10.3389/fpsyt.2021.597894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355422PMC
July 2021

E3 Ubiquitin Ligase March1 Facilitates OX40L Expression in Allergen-Stimulated Dendritic Cells Through Mediating the Ubiquitination of HDAC11.

J Asthma Allergy 2021 5;14:955-966. Epub 2021 Aug 5.

Department of Pediatric Pulmonology, Children's Hospital of Soochow University, Suzhou, Jiangsu, 215003, People's Republic of China.

Background: It was demonstrated that membrane-associated RING-CH 1 (March 1) might play an important role in the pathogenesis of asthma.

Methods: The levels of mRNA and protein were measured by qRT-PCR and Western blot, respectively. Immunofluorescence assay was used to determine whether March1 co-locates with HDAC11. Co-immunoprecipitation was performed to examine the combination of proteins. Moreover, luciferase assay was used to measure the promoter activity of genes.

Results: The mRNA and protein levels of both March1 and OX40 ligand (OX40L) were increased in the dendritic cells (DCs) from asthmatic children and asthmatic animals. Histone deacetylase 11 (HDAC11) protein was decreased in the DCs from asthmatic children and asthmatic model. Increasing of March1 or decreasing of March1 only affect the expression of HDAC11 in protein level. Besides, increasing of HDAC11 could inhibit OX40L expression, and decreasing of HDAC11 promoted OX40L expression in house dust mites (HDMs)-treated DCs. Increasing of HDAC11 notably reversed the promotion of March1 to OX40L expression. Our data further proved that March1 reduced the protein level of HDAC11 through inducing ubiquitination and degradation. HDAC11 combined with krüppel-like factor 4 (KLF4) to decrease the activity of OX40L gene promoter, thus to downregulate the level of OX40L.

Conclusion: Overall, our data showed that HDAC11 promoted KLF4-dependent OX40L decreasing. However, March1 promoted OX40L expression through enhancing the ubiquitination and degradation of HDAC11 and subsequent blocking the inhibition of HDAC11 to OX40L.
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http://dx.doi.org/10.2147/JAA.S318104DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352640PMC
August 2021

Fine-mapping and gene candidate analysis for AhRt1, a major dominant locus responsible for testa color in cultivated peanut.

Theor Appl Genet 2021 Aug 11. Epub 2021 Aug 11.

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs of People's Republic of China, Wuhan, 430062, People's Republic of China.

Key Message: AhRt1 controlling red testa color in peanut was fine-mapped to an interval of 580 kb on chromosome A03, and one gene encoding bHLH transcriptional factor was identified as the putative candidate gene. Peanut with red testa has higher nutritional and economic value than the traditional pink testa varieties. Identification of genes controlling red testa color will accelerate the breeding program and facilitate uncovering the genetic mechanism. In this study, in order to identify gene underlying the red testa color in peanut, a F population derived from a cross between a pink testa peanut variety "Fuhua 8" and a red testa variety "Quanhonghua 1" was constructed. The genetic analysis for the F population revealed that the red testa color was controlled by one single dominant locus. This locus, named as AhRt1 (Arachis hypogaea Red Testa 1), was preliminary identified in chromosome A03 by BSA-sequencing analysis. Using a segregation mapping population, AhRt1 was fine-mapped to a 580-kb genomic region by substitution mapping strategy. Gene candidate analysis suggested that one predicted gene encoding bHLH transcriptional factor may be the possible candidate gene for AhRt1. A diagnostic marker closely linked to candidate gene has been developed for validating the fine-mapping result in different populations and peanut germplasm. Our findings will benefit the breeding program for developing new varieties with red testa color and laid foundation for map-based cloning gene responsible for red testa in peanut.
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http://dx.doi.org/10.1007/s00122-021-03924-wDOI Listing
August 2021

Differences of clinical features and prognosis between Mycoplasma pneumoniae necrotizing pneumonia and non-Mycoplasma pneumoniae necrotizing pneumonia in children.

BMC Infect Dis 2021 Aug 10;21(1):797. Epub 2021 Aug 10.

Department of Respiratory Disease, Children's Hospital of Soochow University, Suzhou, 215003, China.

Background: In the past few years, Mycoplasma pneumoniae (Shi et al. Lancet 390:946-958, 2017) infection has been reported more in China. However, there are few studies on the clinical characteristics and prognosis of necrotizing pneumonia (NP) (Griffiths et al. Nature 583:615-619, 2020) caused by different pathogens.

Methods: A retrospective analysis was performed, including 31 children with a clinical diagnosis of NP in the hospital from January 1, 2013 to January 31, 2020. A total of 11 children with MPNP were included in the observation group and the other 20 children with other pathogens were included in the control group. The clinical manifestations, laboratory data, imaging findings, treatments and outcomes were analyzed.

Results: The proportion of dyspnea cases was significantly higher in the non-Mycoplasma pneumoniae necrotizing pneumonia (N-MPNP) group than that in the Mycoplasma pneumoniae necrotizing pneumonia (MPNP) group (P = 0.02).The LDH level of all patients in the MPNP group was higher than the normal value, with a median value of 805.0 U/L, which was significantly higher than those in the N-MPNP group (414.0 [299.9-540.6] U/L; Z =  - 2.518; P = 0.012). The white blood cells (WBCs) count of the N-MPNP group was 17.8 (11.1-21.7) × 10/L, which was significantly higher than that of the MPNP group (10.2 [6.3-14.1] × 10/L; P < 0.05). The mean time of pulmonary necrosis in the MPNP group was 20.9 ± 6.9 days, which was higher than that of the N-MPNP group (16.8 ± 6.1 days; t = 3.101; P = 0.004). The incidence of pleural effusion in the N-MPNP group (19 patients, 95%) was significantly higher than that in the MPNP group (six patients, 54.55%) (P = 0.013). Among them, two patients received bronchoscopy lavage at a maximum four times, and the cases of plastic bronchitis were seen only in the MPNP group (3 cases; P = 0.037).The length of stay was 18 (10-22) days in the MPNP group and 23.5 (13.5-47) days in the N-MPNP group and no significant difference was observed between the two groups (Z =  - 1.923, P =  - 0.055).

Conclusions: 1. MP infection is the most common infection in children with NP in the Suzhou area. There is no gender and age difference between MPNP and N-MPNP, but the bacterial infection was mainly observed in the N-MPNP group. 2. Children in the N-MPNP group have more severe clinical symptoms, were more prone to shortness of breath, had a longer hospital stay, and had earlier imaging manifestations of necrosis, whereas children in the MPNP group were more likely to have plastic bronchitis. The level of WBC and LDH and the nature of pleural effusion can be used to identify MPNP and N-MPNP to some extent. 3. The prognosis of MPNP was better than that of N-MPNP. There were no death cases. Pleural thickening, pulmonary fibrosis, and bronchiectasis were the most common sequelae. Compared with N-MPNP, the recovery time of lung imaging in MPNP was shorter.
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http://dx.doi.org/10.1186/s12879-021-06469-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8356421PMC
August 2021

Design, synthesis and application of near-infrared fluorescence probe IR-780-Crizotinib in detection of ALK positive tumors.

Protein Expr Purif 2021 Nov 7;187:105952. Epub 2021 Aug 7.

College of Biological and Environmental Engineering, Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, Binzhou University, Binzhou, 256603, China.

At present, the early diagnosis and treatment of NSCLC has become an international research hotspot. However, how to realize the organic combination of highly sensitive and high-resolution tumor imaging diagnosis and effective treatment, and to provide effective information for the diagnosis and treatment of cancer is still a major problem in the integration of cancer diagnosis and treatment. In this study, based on the Crizotinib has a good targeted inhibitory effect on ALK positive tumor cells, the near-infrared targeted fluorescent dye IR-780 was covalently bound with the drug molecule Crizotinib, thus the near-infrared fluorescent probe IR-780-Crizotinib targeting ALK positive tumor cells was synthesized. The probe structure is confirmed by NMR and MS. The optical properties of the fluorescent probe and the imaging process in ALK positive tumor-bearing mice were analyzed using ultraviolet spectrophotometer, near-infrared fluorescence spectrometer, and near-infrared fluorescence imaging system. The results show that the probe had better photoactivity. In vivo imaging shows that the probe maintained the biological activity of Crizotinib, effectively targeting the tumor site involved with clear imaging, and ultimately excreted from the body. It was confirmed that the probe could be used for the tracking, positioning and targeted therapy of nude mice with ALK positive tumors in vivo, thus exploring a new approach for the clinical application of near-infrared fluorescent probe to detect ALK positive tumors in the future.
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http://dx.doi.org/10.1016/j.pep.2021.105952DOI Listing
November 2021

Acupuncture for glucose and lipid metabolic disorders of polycystic ovarian syndrome: A systematic review protocol.

PLoS One 2021 5;16(8):e0255732. Epub 2021 Aug 5.

Department of Gynecology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, P.R. China.

Background: Polycystic ovary syndrome (PCOS) is a common gynecological disease that is often accompanied by some metabolic abnormality such as insulin resistance and dyslipidemia. As a non-pharmacological therapy, acupuncture is widely used for the treatment of PCOS, but the effectiveness for insulin resistance and lipid metabolic disorder remains controversial.

Objectives: To assess the effectiveness and safety of acupuncture for insulin resistance and lipid metabolic disorder of women with PCOS.

Search Methods: Eight databases will be searched from inception to June 2021, three clinical trial registration platforms will be searched for relevant trials.

Selection Criteria: Randomized controlled trials (RCTs) of acupuncture therapy for insulin resistance and lipid metabolic of PCOS will be included.

Data Collection And Analysis: Study screening, data collection, and analysis will be performed by two or more reviewers independently. We will calculate mean difference (MD), standard mean difference (SMD) with 95% confidence intervals (CIs). Data synthesis will be performed with RevMan V.5.3 software and with Stata V.15.0 software when necessary.

Prospero Registration Number: CRD42020177846.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0255732PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341540PMC
August 2021

Systemic immune-inflammation index predicts a reduced risk of end-stage renal disease in Chinese patients with myeloperoxidase-anti-neutrophil cytoplasmic antibody-associated vasculitis: A retrospective observational study.

Exp Ther Med 2021 Sep 13;22(3):989. Epub 2021 Jul 13.

Department of Nephrology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.

Chronic inflammation has been indicated to be important in the pathogenesis of anti-neutrophil cytoplasmic antibody-associated vasculitis (AAV). The systemic immune-inflammation index (SII) is a novel marker of inflammation. The present study was thus performed to explore the association between the SII at diagnosis and inflammatory response and disease activity in Chinese patients with myeloperoxidase (MPO)-AAV. Furthermore, it was evaluated whether the SII is able to predict the progression to end-stage renal disease (ESRD) and patient survival. A total of 190 patients with MPO-AAV were included in the present study. The baseline SII was positively correlated with C-reactive protein (CRP; r=0.274, P<0.0001) and the erythrocyte sedimentation rate (ESR; r=0.481, P<0.0001). However, the SII had no obvious correlation with the Birmingham vasculitis activity score. Patients with SII≥2,136.45 exhibited better cumulative renal survival rates than those with SII<2,136.45 (P=0.001). However, no significant difference in patient survival was indicated between patients with SII≥2,136.45 and those with SII<2,136.45 at diagnosis. In conclusion, the SII was positively correlated with CRP and ESR in Chinese patients with MPO-AAV. Furthermore, the SII may be an independent factor associated with a reduced risk of ESRD.
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http://dx.doi.org/10.3892/etm.2021.10421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8311255PMC
September 2021
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