Publications by authors named "Li He"

2,190 Publications

  • Page 1 of 1

A male-ABCD algorithm for hepatocellular carcinoma risk prediction in HBsAg carriers.

Chin J Cancer Res 2021 Jun;33(3):352-363

State Key Lab of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

Objective: Hepatocellular carcinoma (HCC) development among hepatitis B surface antigen (HBsAg) carriers shows gender disparity, influenced by underlying liver diseases that display variations in laboratory tests. We aimed to construct a risk-stratified HCC prediction model for HBsAg-positive male adults.

Methods: HBsAg-positive males of 35-69 years old (N=6,153) were included from a multi-center population-based liver cancer screening study. Randomly, three centers were set as training, the other three centers as validation. Within 2 years since initiation, we administrated at least two rounds of HCC screening using B-ultrasonography and α-fetoprotein (AFP). We used logistic regression models to determine potential risk factors, built and examined the operating characteristics of a point-based algorithm for HCC risk prediction.

Results: With 2 years of follow-up, 302 HCC cases were diagnosed. A male-ABCD algorithm was constructed including participant's age, blood levels of GGT (γ-glutamyl-transpeptidase), counts of platelets, white cells, concentration of DCP (des-γ-carboxy-prothrombin) and AFP, with scores ranging from 0 to 18.3. The area under receiver operating characteristic was 0.91 (0.90-0.93), larger than existing models. At 1.5 points of risk score, 26.10% of the participants in training cohort and 14.94% in validation cohort were recognized at low risk, with sensitivity of identifying HCC remained 100%. At 2.5 points, 46.51% of the participants in training cohort and 33.68% in validation cohort were recognized at low risk with 99.06% and 97.78% of sensitivity, respectively. At 4.5 points, only 20.86% of participants in training cohort and 23.73% in validation cohort were recognized at high risk, with positive prediction value of 22.85% and 12.35%, respectively.

Conclusions: Male-ABCD algorithm identified individual's risk for HCC occurrence within short term for their HCC precision surveillance.
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http://dx.doi.org/10.21147/j.issn.1000-9604.2021.03.07DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8286891PMC
June 2021

B,N-Doped PdRu Aerogels as High-Performance Peroxidase Mimics for Sensitive Detection of Glucose.

ACS Appl Mater Interfaces 2021 Jul 28. Epub 2021 Jul 28.

College of Optoelectronic Engineering, Chengdu University of Information Technology, Chengdu 610225, China.

Among plentiful porous nanomaterials, noble metal aerogels taken as nanozymes attract broad attention in sensing applications with their distinct enzyme mimic functions. In the catalytic field, the heteroatom doping strategy is a kind of way with great promise in improving the enzyme mimic activity of noble metal aerogels. In this experiment, we find a type of creative materials that were prepared by the fast and simple method. Due to the unique porous structure and synergetic effect from doped atoms, PdRu aerogels co-doped with boron and nitrogen (B, N-PdRu aerogels) were prepared using NHBH as a reductant, which present improved peroxidase mimicking activity. With the existence of HO, the oxidation of 3,3',5,5'-tetramethylbenzidine was catalyzed by B, N-PdRu aerogels fairly efficiently, whose solution would be a blue appearance at optimum absorption wavelength 652 nm. Thus, by the tandem reaction bound to the enzyme glucose oxidase, the B, N-PdRu aerogels can be used for the sensitive determination of glucose. The new method has a good linear detection effect for glucose in the range of 10 μM to 2 mM. The minimum limit of detection can reach as low as 6 μM. This work will contribute to research on the rational design of metal aerogels based on the heteroatomic doping strategy and enhance the corresponding performance for a variety of applications.
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http://dx.doi.org/10.1021/acsami.1c07987DOI Listing
July 2021

Super enhancer regulation of cytokine-induced chemokine production in alcoholic hepatitis.

Nat Commun 2021 07 27;12(1):4560. Epub 2021 Jul 27.

Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA.

Alcoholic hepatitis (AH) is associated with liver neutrophil infiltration through activated cytokine pathways leading to elevated chemokine expression. Super-enhancers are expansive regulatory elements driving augmented gene expression. Here, we explore the mechanistic role of super-enhancers linking cytokine TNFα with chemokine amplification in AH. RNA-seq and histone modification ChIP-seq of human liver explants show upregulation of multiple CXCL chemokines in AH. Liver sinusoidal endothelial cells (LSEC) are identified as an important source of CXCL expression in human liver, regulated by TNFα/NF-κB signaling. A super-enhancer is identified for multiple CXCL genes by multiple approaches. dCas9-KRAB-mediated epigenome editing or pharmacologic inhibition of Bromodomain and Extraterminal (BET) proteins, transcriptional regulators vital to super-enhancer function, decreases chemokine expression in vitro and decreases neutrophil infiltration in murine models of AH. Our findings highlight the role of super-enhancer in propagating inflammatory signaling by inducing chemokine expression and the therapeutic potential of BET inhibition in AH treatment.
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http://dx.doi.org/10.1038/s41467-021-24843-wDOI Listing
July 2021

Risk factors for gastric cancer: a large-scale, population-based case-control study.

Chin Med J (Engl) 2021 Jul 22. Epub 2021 Jul 22.

Office of Cancer Screening, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College/Chinese Academy of Medical Sciences Key Laboratory for National Cancer Big Data Analysis and Implement, Beijing 100021, China Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

Background: Early detection of gastric cancer (GC) has been the topic of major efforts in China. This study aimed to explore the risk factors associated with GC and to provide evidence for the selection of a high-risk population of GC.

Methods: Based on the cancer screening cohort of the National Cancer Screening Program in Urban China, GC patients diagnosed by endoscopy and pathological examinations constituted the case group, and controls were 1:3 matched by sex and age (±5 years) individually. The variables were selected by univariable analysis of factors such as body mass index (BMI), dietary habits, lifestyle, stomach disease history, and family history of GC; and multivariable logistic regression was used to analyze the influencing factors of GC and to calculate the odds ratio (OR) of related factors and its 95% confidence interval (CI).

Results: A total of 215 GC cases and 645 matched healthy controls were included in the final analysis, with a median age of 61 years for the case and control groups. Overall analysis showed that high educational level (above primary school) (OR = 0.362, 95% CI = 0.219-0.599, P < 0.001), overweight/obesity (BMI ≥24 kg/m2; OR = 0.489, 95% CI = 0.329-0.726, P < 0.001), cigarette smoking (OR = 3.069, 95% CI = 1.700-5.540, P < 0.001), alcohol consumption (OR = 1.661, 95% CI = 1.028-2.683, P = 0.038), history of stomach disease (OR = 6.917, 95% CI = 4.594-10.416, P < 0.001), and family history of GC in first-degree relatives (OR = 4.291, 95% CI = 1.661-11.084, P = 0.003) were significantly correlated with the occurrence of GC. Subgroup analyses by age and gender indicated that GC risk was still increased in the presence of a history of stomach disease. A history of chronic gastritis, gastric ulcer, or gastric polyposis was positively associated with GC, with adjusted ORs of 4.155 (95% CI = 2.711-6.368), 1.839 (95% CI = 1.028-3.288), and 2.752 (95% CI = 1.197-6.326).

Conclusions: Subjects who smoke, drink, with history of stomach disease and family history of GC in first-degree relatives are the high-risk populations for GC. Therefore, attention should be paid to these subjects for GC screening.
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http://dx.doi.org/10.1097/CM9.0000000000001652DOI Listing
July 2021

Determining the Traditional Chinese Medicine (TCM) Syndrome with the Best Prognosis of HBV-Related HCC and Exploring the Related Mechanism Using Network Pharmacology.

Evid Based Complement Alternat Med 2021 29;2021:9991533. Epub 2021 Jun 29.

The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine (Shenzhen Traditional Chinese Medicine Hospital), Shenzhen 518033, China.

Background: In traditional Chinese medicine (TCM), TCM syndrome is a key guideline, and Chinese materia medicas are widely used to treat hepatitis B virus- (HBV-) related hepatocellular carcinoma (HCC) according to different TCM syndromes. However, the prognostic value of TCM syndromes in HBV-related HCC patients has never been studied.

Methods: A retrospective cohort of HBV-related HCC patients at Shenzhen Traditional Chinese Medicine Hospital from December 2005 to October 2017 was analyzed. The prognostic value of TCM syndromes in HBV-related HCC patients was assessed by Kaplan-Meier survival curves and Cox analysis, and the TCM syndrome with the best prognosis of HBV-related HCC patients was determined. To further study the relevant mechanisms, key Chinese materia medicas (KCMMs) for the TCM syndrome with the best prognosis were summarized, and network pharmacology was also performed.

Results: A total of 207 HBV-related HCC patients were included in this research, and we found that HBV-related HCC patients with TCM excess syndrome had better OS. Then, a total of eight KCMMs for TCM excess syndrome were identified, whose crucial ingredients included quercetin, beta-sitosterol, kaempferol, luteolin, and XH-14, and KCMMs could play a therapeutic role through MAPK, JAK-STAT, Wnt, Hippo, and other pathways. Moreover, TP53, SRC, STAT3, MAPK3, PIK3R1, HRAS, VEGFA, HSP90AA1, EGFR, and JAK2 were determined as the key targets.

Conclusion: We propose a new research method of "prognosis of TCM syndromes-KCMMs-network pharmacology" to reveal the prognostic value of TCM syndromes and the potential mechanism by which TCM syndromes affect prognosis.
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http://dx.doi.org/10.1155/2021/9991533DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263254PMC
June 2021

Tailoring Highly Ordered Graphene Framework in Epoxy for High-Performance Polymer-Based Heat Dissipation Plates.

ACS Nano 2021 Jul 26. Epub 2021 Jul 26.

Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo 315201, People's Republic of China.

As the power density and integration level of electronic devices increase, there are growing demands to improve the thermal conductivity of polymers for addressing the thermal management issues. On the basis of the ultrahigh intrinsic thermal conductivity, graphene has exhibited great potential as reinforcing fillers to develop polymer composites, but the resultant thermal conductivity of reported graphene-based composites is still limited. Here, an interconnected and highly ordered graphene framework (HOGF) composed of high-quality and horizontally aligned graphene sheets was developed by a porous film-templated assembly strategy, followed by a stress-induced orientation process and graphitization post-treatment. After embedding into the epoxy (EP), the HOGF/EP composite (24.7 vol %) exhibits a record-high in-plane thermal conductivity of 117 W m K, equivalent to ≈616 times higher than that of neat epoxy. This thermal conductivity enhancement is mainly because the HOGF as a filler concurrently has high intrinsic thermal conductivity, relatively high density, and a highly ordered structure, constructing superefficient phonon transport paths in the epoxy matrix. Additionally, the use of our HOGF/EP as a heat dissipation plate was demonstrated, and it achieved 75% enhancement in practical thermal management performance compared to that of conventional alumina for cooling the high-power LED.
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http://dx.doi.org/10.1021/acsnano.1c01332DOI Listing
July 2021

Giant blood cyst, isolated dysgenesia of pulmonary valve and pulmonary artery aneurysm: a rare combination.

Int J Cardiovasc Imaging 2021 Jul 25. Epub 2021 Jul 25.

Department of Ultrasound, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

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http://dx.doi.org/10.1007/s10554-021-02288-yDOI Listing
July 2021

The Combination of Concentrated Growth Factor and Adipose-Derived Stem Cell Sheet Repairs Skull Defects in Rats.

Tissue Eng Regen Med 2021 Jul 24. Epub 2021 Jul 24.

Department of Stomatology, Renmin Hospital, Hubei University of Medicine, NO.39 Chaoyang Middle Road, Maojian District, Shiyan, 442000, Hubei, China.

Background: The goal of this study was to create a biomaterial which combines concentrated growth factor (CGF) with an adipose-derived stem cell (ADSC) sheet to promote the repair of skull defects in rats.

Methods: We determined the optimal concentration of CGF extract by investigating the effects of different concentrations (0, 5%, 10%, and 20%) on the proliferation and differentiation of ADSCs. Then we created a complex combining CGF with an ADSC sheet, and tested the effects on bone repair in four experimental rat groups: (A) control; (B) ADSC sheet; (C) CGF particles; (D) combination of CGF + ADSCs. Eight weeks after the procedure, osteogenesis was assessed by micro-CT and hematoxylin and eosin staining.

Results: We found that the concentration of CGF extract that promoted optimal ADSC proliferation and differentiation in vitro was 20%. In turn, bone regeneration was promoted the most by the combination of CGF and ADSCs.

Conclusion: In this study, we determined the optimal ratio of CGF and ADSCs to be used in a biomaterial for bone regeneration. The resulting CGF/ADSCs complex promotes maxillofacial bone defect repair in rats.
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http://dx.doi.org/10.1007/s13770-021-00371-yDOI Listing
July 2021

Physicochemical Characterization and Biopharmaceutical Evaluation of ZWF: A Novel Anticancer Drug for the Treatment of Non-small Cell Lung Cancer.

AAPS PharmSciTech 2021 Jul 23;22(6):207. Epub 2021 Jul 23.

Key Laboratory of Drug-Targeting and Drug Delivery Systems of the Education Ministry, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu, 610041, People's Republic of China.

The orally available novel small molecule drug ZWF is under preclinical development for an anticancer purpose. The present study aimed to assess the viability of developing ZWF as a form of oral formulation for clinical application based on the principles of biopharmaceutics and pharmacokinetics. The crucial physicochemical properties of ZWF were determined by in vitro assays. The in situ gastrointestinal absorption characteristics and in vivo pharmacokinetic behaviors of ZWF in rats were characterized. The solubility of ZWF showed a highly pH-dependent profile, decreasing from 25,392.89 to 20.48 μg/mL as the solution pH increased from 1.0 to 5.8. In PBS with a pH of 1.0 to 5.8, the LogP value of ZWF ranged from -2.35 to 2.20 and was gradually increased as the pH value increased. ZWF was partially absorbed in the stomach, and the favorable absorption sites were the duodenum, jejunum, and ileum. Pharmacokinetic studies showed that the AUC and C values of ZWF after its oral administration as a suspension prepared with 0.5% CMC-Na were increased by 18.97% and 40% than that with normal saline, providing a model oral formulation of ZWF with ideal bioavailability and system exposure in rats. From the perspective of oral absorption, ZWF possessed appealing qualities as a drug candidate and could be prepared as an oral preparation for clinical application. The present study has established a fundamental foundation for the development and quality evaluation of the ZWF oral formulations.
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http://dx.doi.org/10.1208/s12249-021-02084-wDOI Listing
July 2021

Single-Cell Analysis Reveals the Heterogeneity of Monocyte-Derived and Peripheral Type-2 Conventional Dendritic Cells.

J Immunol 2021 Aug 19;207(3):837-848. Epub 2021 Jul 19.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, China;

Dendritic cells (DCs) are critical for pathogen recognition and Ag processing/presentation. Human monocyte-derived DCs (moDCs) have been extensively used in experimental studies and DC-based immunotherapy approaches. However, the extent of human moDC and peripheral DCs heterogeneity and their interrelationship remain elusive. In this study, we performed single-cell RNA sequencing of human moDCs and blood DCs. We identified seven subtypes within moDCs: five corresponded to type 2 conventional DCs (cDC2s), and the other two were CLEC10ACD127 cells with no resemblance to any peripheral DC subpopulations characterized to date. Moreover, we defined five similar subtypes in human cDC2s, revealed the potential differentiation trajectory among them, and unveiled the transcriptomic differences between moDCs and cDC2s. We further studied the transcriptomic changes of each moDC subtype during maturation, demonstrating and as maturation markers and and as markers for immature DCs. These findings will enable more accurate functional/developmental analyses of human cDC2s and moDCs.
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http://dx.doi.org/10.4049/jimmunol.2100094DOI Listing
August 2021

Risk Perception and Coping Behavior of Construction Workers on Occupational Health Risks-A Case Study of Nanjing, China.

Int J Environ Res Public Health 2021 07 1;18(13). Epub 2021 Jul 1.

Department of Construction and Real Estate, School of Civil Engineering, Southeast University, Nanjing 210096, China.

To reduce harm caused by occupational health risks of construction workers exposed to working environments, especially those for interior decoration, it is crucial for them to actively recognize and prevent these risks. Therefore, how to improve their occupational health risks perception and regulate their coping behaviors should be of great concern. However, most prior studies target construction worker safety, and little research focuses on risk analysis from the psychological level of workers. Hence, construction workers' occupational health risk perception level and coping behavior level in Nanjing and the influencing factors were analyzed through statistical analysis with 341 valid questionnaires. Bootstrapping was applied to test the mediating effects of risk perception on the proposed factors and coping behaviors. This study revealed that construction workers have a high-level of occupational health risk perception, yet low-level coping behavior. Gender, age, education level, and unit qualification cause differences in individual risk perception level. Personal knowledge and group effects significantly affect the level of risk perception, which subsequently affect coping behavior. Education level, monthly income, and personal knowledge influence the coping behavior through risk perception. Recommendations were put forward for risk perception and coping behavior improvement from the perspectives of construction workers themselves, enterprises, and governments. This study sheds new light for research areas of occupational health and risk management and provides beneficial practice for improving construction workers' responses to occupational health risks.
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http://dx.doi.org/10.3390/ijerph18137040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297174PMC
July 2021

Updating the NLRC4 Inflammasome: from Bacterial Infections to Autoimmunity and Cancer.

Front Immunol 2021 30;12:702527. Epub 2021 Jun 30.

Department of Central Laboratory, First Hospital of Qinhuangdao, Hebei Medical University, Qinhuangdao, China.

Inflammasomes comprise a family of cytosolic multi-protein complexes that modulate the activation of cysteine-aspartate-specific protease 1 (caspase-1) and promote the maturation and secretion of interleukin (IL)-1β and IL-18, leading to an inflammatory response. Different types of inflammasomes are defined by their sensor protein which recognizes pathogenic ligands and then directs inflammasome assembly. Although the specific molecular mechanisms underlying the activation of most inflammasomes are still unclear, NLRC4 inflammasomes have emerged as multifaceted agents of the innate immune response, playing important roles in immune defense against a variety of pathogens. Other studies have also expanded the scope of NLRC4 inflammasomes to include a range of inherited human autoimmune diseases as well as proposed roles in cancer. In this review article, we provide an updated overview of NLRC4 inflammasomes, describing their composition, activation mechanisms and roles in both microbial infections and other disease conditions.
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http://dx.doi.org/10.3389/fimmu.2021.702527DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283967PMC
June 2021

Research progress in the treatment of partial platinum-sensitive recurrent ovarian cancer.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Jun;46(6):644-652

Gynecological Oncology Research Center of Hunan Cancer Hospital, Changsha 410013, China.

Ovarian cancer is a common malignant tumor in gynaecology and its mortality rate is the highest in gynecological cancer. Ovarian cancer patients should face platinum resistance, caused by multiple recurrences. According to the platinum-free interval (PFI), recurrent ovarian cancer can be divided into platinum-resistant (PFI<6 months) and platinum-sensitive (PFI≥6 months). When PFI is 6-12 months, it belongs to the partial platinum-sensitive ovarian cancer. At present, how to prolong the survival of patients with partial platinum-sensitive recurrent ovarian cancer is difficult. Secondary cytoreductive surgery, non-platinum chemotherapy, anti-angiogenesis drugs, and molecular targeted therapy of poly (ADP ribose) polymerase (PARP) inhibitors can improve the prognosis of patients with partial platinum-sensitive recurrent ovarian cancer.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2021.200494DOI Listing
June 2021

Prognostic role of programmed cell death ligand-1 expression in head and neck cancer treated with programmed cell death protein-1/programmed cell death ligand-1 inhibitors: A meta-analysis based on clinical trials.

J Cancer Res Ther 2021 Jul;17(3):676-687

Collaborative Innovation Center for Cancer Medicine, State Key Laboratory of Oncology in South China; Sun Yat-Sen University Cancer Center, Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy; Department of Radiation Oncology, Sun Yat-Sen University Cancer Center, Guangzhou, China.

Purpose: The purpose of our meta-analysis is to clarify whether the biomarker of programmed cell death ligand-1 (PD-L1) could predict the treatment efficacy and prognosis of programmed cell death protein-1 (PD-1)/PD-L1 immune-checkpoint inhibitors (ICIs) in head and neck cancer (HNC) patients.

Materials And Methods: We performed the article search in four main online databases. The search deadline was September 8, 2020. To elucidate whether a positive or negative PD-L1 expression correlates with different efficacy and prognosis of PD-1/PD-L1-related therapy in HNC, the relative risk (RR) and 95% confidence interval (95% CI) were pooled. Our meta-analysis assigned the overall survival (OS) at 6 and 12 months and the objective response rate (ORR) for the primary end points.

Results: The present meta-analysis included 11 relevant studies, which have 1663 HNC cases who received the treatment of PD-1/PD-L1 ICIs. The pooled results revealed that the high or positive expression of PD-L1 predicted better 6- and 12-month OS in head and neck squamous cell carcinoma (HNSCC) (RR 1.30, 95% CI: 1.02-1.65, P = 0.03; and RR 1.31, 95% CI: 1.05-1.62, P = 0.01). PD-L1 expressors were also relevant with higher ORR in HNC patients who had treatment of PD-1/PD-L1 inhibitors compared to PD-L1 nonexpressors (RR 1.84, 95% CI: 1.41-2.41, P < 0.00001).

Conclusions: In summary, PD-L1-positive HNSCC patients portend favorable OS at 6 and 12 months from PD-1/PD-L1 ICIs. Increased ORR also favored to appear in PD-L1 expressors of HNC or recurrent/metastatic HNSCC who received PD-1/PD-L1 ICIs. Therefore, PD-L1 proved to be an appropriate biomarker to predict the clinical efficacy and prognosis of PD-1/PD-L1 ICIs in HNC.
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http://dx.doi.org/10.4103/jcrt.JCRT_1606_20DOI Listing
July 2021

Arylethynyltrifluoroborate Dienophiles for on Demand Activation of IEDDA Reactions.

Bioconjug Chem 2021 Jul 15. Epub 2021 Jul 15.

Yusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, CB2 1EW Cambridge, United Kingdom.

Strained alkenes and alkynes are the predominant dienophiles used in inverse electron demand Diels-Alder (IEDDA) reactions. However, their instability, cross-reactivity, and accessibility are problematic. Unstrained dienophiles, although physiologically stable and synthetically accessible, react with tetrazines significantly slower relative to strained variants. Here we report the development of potassium arylethynyltrifluoroborates as unstrained dienophiles for fast, chemically triggered IEDDA reactions. By varying the substituents on the tetrazine (e.g., pyridyl- to benzyl-substituents), cycloaddition kinetics can vary from fast ( = 21 M s) to no reaction with an alkyne-BF dienophile. The reported system was applied to protein labeling both in the test tube and fixed cells and even enabled mutually orthogonal labeling of two distinct proteins.
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http://dx.doi.org/10.1021/acs.bioconjchem.1c00276DOI Listing
July 2021

High-Efficiency Spatial-Wave Frequency Multiplication Using Strongly Nonlinear Metasurface.

Adv Sci (Weinh) 2021 Jul 15:e2101212. Epub 2021 Jul 15.

State Key Laboratory of Millimeter Waves, Southeast University, Nanjing, 210096, China.

In the past decades, metasurfaces have opened up a promising venue for manipulating lights and electromagnetic (EM) waves. In the field of nonlinearity, second-harmonic generation (SHG) is a research focus due to its diverse applications. There have been many researches for realizing SHG in optical regime using nonlinear characteristics of optical materials, but its efficiency is low. In microwave frequencies, SHGs are basically studied in the guided-wave systems. Here, high-efficiency SHGs of spatial waves are presented in the microwave frequency using nonlinear metasurface loaded with active chips at the subwavelength scale. The nonlinear meta-atom is composed of receiving antenna, transmitting antenna, and active circuit of frequency multiplier, which can realize strongly nonlinear response and link the EM signals from the receiving to transmitting antennas. Correspondingly, to achieve the function of spatial-wave frequency multiplication, the working frequency of the transmitting antenna in the meta-atom should be twice as that of the receiving antenna, and hence the active chip is well matched to obtain the signal transforming with high efficiency. Good performance of the spatial-wave frequency multiplication is demonstrated in the proof-of-concept experiments with the best transform efficiency of 85.11% under normal incidence, validating the proposed method.
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http://dx.doi.org/10.1002/advs.202101212DOI Listing
July 2021

Pharmacological and genetic manipulations at the µ-opioid receptor reveal arrestin-3 engagement limits analgesic tolerance and does not exacerbate respiratory depression in mice.

Neuropsychopharmacology 2021 Jul 13. Epub 2021 Jul 13.

Center for Neuroscience, University of California-Davis, Davis, CA, USA.

Opioid drugs are widely used analgesics that activate the G protein-coupled µ-opioid receptor, whose endogenous neuropeptide agonists, endorphins and enkephalins, are potent pain relievers. The therapeutic utility of opioid drugs is hindered by development of tolerance to the analgesic effects, requiring dose escalation for persistent pain control and leading to overdose and fatal respiratory distress. The prevailing hypothesis is that the intended analgesic effects of opioid drugs are mediated by µ-opioid receptor signaling to G protein, while the side-effects of respiratory depression and analgesic tolerance are caused by engagement of the receptor with the arrestin-3 protein. Consequently, opioid drug development has focused exclusively on identifying agonists devoid of arrestin-3 engagement. Here, we challenge the prevailing hypothesis with a panel of six clinically relevant opioid drugs and mice of three distinct genotypes with varying abilities to promote morphine-mediated arrestin-3 engagement. With this genetic and pharmacological approach, we demonstrate that arrestin-3 recruitment does not impact respiratory depression, and effective arrestin-3 engagement reduces, rather than exacerbates, the development of analgesic tolerance. These studies suggest that future development of safer opioids should focus on identifying opioid ligands that recruit both G protein and arrestin-3, thereby mimicking the signaling profile of most endogenous µ-opioid receptor agonists.
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http://dx.doi.org/10.1038/s41386-021-01054-xDOI Listing
July 2021

Enhanced sludge dewaterability by Fe-rich biochar activating hydrogen peroxide: Co-hydrothermal red mud and reed straw.

J Environ Manage 2021 Jul 9;296:113239. Epub 2021 Jul 9.

School of Resource and Environmental Science, Wuhan University, Wuhan, 430072, Hubei, China; College of Resources and Environment, Anqing Normal University, Anqing, 246011, Anhui, China. Electronic address:

This study proposed Fe-rich biochar (RMRS-BC) produced by the co-hydrothermal treatment of red mud and reed straw, industrial waste and agricultural waste, as a novel sludge conditioner. It had been proven that heterogeneous and homogeneous Fenton reactions occurred during the sludge conditioning process, in which RMRS-BC activated HO to improve sludge dewaterability. Results demonstrated that the optimal condition was 7.5 wt% dry solids (DS) of RMRS-BC at a mass ratio of 1:1 combined with HO. The corresponding water content of sludge cakes and the capillary suction time reduction efficiency were 57.88 wt% and 69.76%, respectively. The FeO supported in the RMRS-BC structure was used as a catalyst to produce heterogeneous reaction, and the Fe leached from the RMRS-BC after acidification happened homogeneous reaction. Double Fenton reaction in sludge conditioning enhanced the production efficiency of ·OH, the sludge flocs were dispersed into smaller particles, more bound water from the extracellular polymeric substances (EPS) was released, and sludge dewaterability performance was improved. Another main mechanism for enhancing dewaterability was to use RMRS-BC as a skeleton builder to reduce the compressibility of sludge cakes and facilitated free water to flow out. In summary, the Fenton oxidation method activated by RMRS-BC is feasible in improving sludge dewatering.
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http://dx.doi.org/10.1016/j.jenvman.2021.113239DOI Listing
July 2021

E183K Mutation in Chalcone Synthase C2 Causes Protein Aggregation and Maize Colorless.

Front Plant Sci 2021 23;12:679654. Epub 2021 Jun 23.

College of Plant Science, Jilin University, Changchun, China.

Flavonoids give plants their rich colors and play roles in a number of physiological processes. In this study, we identified a novel colorless maize mutant showing reduced pigmentation throughout the whole life cycle by EMS mutagenesis. E183K mutation in maize chalcone synthase C2 (ZmC2) was mapped using MutMap strategy as the causal for colorless, which was further validated by transformation in Arabidopsis. We evaluated transcriptomic and metabolic changes in maize first sheaths caused by the mutation. The downstream biosynthesis was blocked while very few genes changed their expression pattern. ZmC2-E183 site is highly conserved in chalcone synthase among Plantae kingdom and within species' different varieties. Through prokaryotic expression, transient expression in maize leaf protoplasts and stable expression in Arabidopsis, we observed that E183K and other mutations on E183 could cause almost complete protein aggregation of chalcone synthase. Our findings will benefit the characterization of flavonoid biosynthesis and contribute to the body of knowledge on protein aggregation in plants.
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http://dx.doi.org/10.3389/fpls.2021.679654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261305PMC
June 2021

The Histone Acetyltransferase CfGcn5 Regulates Growth, Development, and Pathogenicity in the Anthracnose Fungus on the Tea-Oil Tree.

Front Microbiol 2021 23;12:680415. Epub 2021 Jun 23.

College of Forestry, Central South University of Forestry and Technology, Changsha, China.

The tea-oil tree ( Abel.) is a commercial edible-oil tree in China, and anthracnose commonly occurs in its plantations, causing great losses annually. We have previously revealed that CfSnf1 is essential for pathogenicity in , the major pathogen of anthracnose on the tea-oil tree. Here, we identified CfGcn5 as the homolog of yeast histone acetyltransferase ScGcn5, which cooperates with ScSnf1 to modify histone H3 in . Targeted gene deletion revealed that CfGcn5 is important in fungi growth, conidiation, and responses to environmental stresses. Pathogenicity assays indicated that CfGcn5 is essential for virulence both in unwounded and wounded tea-oil tree leaves. Further, we found that CfGcn5 is localized to the nucleus and this specific localization is dependent on both NLS region and HAT domain. Moreover, we provided evidence showing that the nuclear localization is essential but not sufficient for the full function of CfGcn5, and the NLS, HAT, and Bromo domains were proven to be important for normal CfGcn5 functions. Taken together, our studies not only illustrate the key functions of CfGcn5 in growth, development, and pathogenicity but also highlight the relationship between its locations with functions in .
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http://dx.doi.org/10.3389/fmicb.2021.680415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260702PMC
June 2021

Association of Aortic Stiffness and Cognitive Decline: A Systematic Review and Meta-Analysis.

Front Aging Neurosci 2021 24;13:680205. Epub 2021 Jun 24.

Department of Neurology, West China Hospital, Sichuan University, Chengdu, China.

Increased aortic stiffness has been found to be associated with cognitive function decline, but the evidence is still under debate. It is of great significance to elucidate the evidence in this debate to help make primary prevention decisions to slow cognitive decline in our routine clinical practice. Electronic databases of PubMed, EMBASE, and Cochrane Library were systematically searched to identify peer-reviewed articles published in English from January 1, 1986, to March 16, 2020, that reported the association between aortic stiffness and cognitive function. Studies that reported the association between aortic pulse wave velocity (PWV) and cognitive function, cognitive impairment, and dementia were included in the analysis. Thirty-nine studies were included in the qualitative analysis, and 29 studies were included in the quantitative analysis. The aortic PWV was inversely associated with memory and processing speed in the cross-sectional analysis. In the longitudinal analysis, the high category of aortic PWV was 44% increased risk of cognitive impairment (OR 1.44; 95% CI 1.24-1.85) compared with low PWV, and the risk of cognitive impairment increased 3.9% (OR 1.039; 95% CI 1.005-1.073) per 1 m/s increase in aortic PWV. Besides, meta-regression analysis showed that age significantly increased the association between high aortic PWV and cognitive impairment risk. Aortic stiffness measured by aortic PWV was inversely associated with memory and processing speed and could be an independent predictor for cognitive impairment, especially for older individuals.
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http://dx.doi.org/10.3389/fnagi.2021.680205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261283PMC
June 2021

Mental health first aid improves mental health literacy among college students: A meta-analysis.

J Am Coll Health 2021 Jul 9:1-10. Epub 2021 Jul 9.

Clinical Nursing Teaching and Research Section, the Second Xiangya Hospital, Central South University, Changsha, Hunan Province, Republic of China.

Objectives: Mental health first aid (MHFA) may have beneficial effects on the public's knowledge, attitude, and behavior; however, its effectiveness in increasing mental health literacy on college students remains unknown.

Methods: We systematically searched the PubMed, EMBASE, and Cochrane Library databases from inception to January 2020. We included trials that compared the effect between the MHFA program group and control group on the knowledge, stigmatizing attitudes, confidence, and intention of college students. A random-effects model was used.

Results: We analyzed five trials involving 1134 participants and found that the MHFA program could significantly increase the student's knowledge (SMD: 0.49, 95% CI: [0.28-0.70]) and confidence (SMD: 0.71, 95% CI: 0.24-1.19).

Conclusions: Overall, the MHFA program could increase college students' knowledge regarding mental health and confidence to support people with mental health problems. However, well-designed control trials are required to investigate the program's effect on mental health literacy in college students.

Abbreviations: MHFA, Mental health first aid; GPs, General Practioners; CI, confidence interval; SMD, standardized mean24difference.
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http://dx.doi.org/10.1080/07448481.2021.1925286DOI Listing
July 2021

Pregnancy and childbirth after transvaginal natural orifice transluminal endoscopic surgery for benign gynecological diseases.

Authors:
Dan Feng Li He

Int J Gynaecol Obstet 2021 Jul 9. Epub 2021 Jul 9.

Department Gynecology, Chengdu Women's & Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China.

In recent years, natural orifice transluminal endoscopic surgery (NOTES) has been utilized as an extended innovation of conventional laparoscopy. Due to its natural advantages, such as easy access to the peritoneal cavity and safe closure of the incision, transvaginal natural orifice transluminal endoscopic surgery (vNOTES) has become a popular surgical treatment method.
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http://dx.doi.org/10.1002/ijgo.13820DOI Listing
July 2021

The Efficacy of Adalimumab as an Initial Treatment in Patients with Behçet's Retinal Vasculitis.

Front Pharmacol 2021 22;12:609148. Epub 2021 Jun 22.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

No study has evaluated the effectiveness of Adalimumab (ADA) as first-line in treatment-naïve patients with retinal vasculitis due to Behçet's Uveitis (BU). To compare the efficacy of ADA plus conventional therapy and conventional therapy alone as initial treatments in naïve BU patients characterized by retinal vasculitis. Medical records of BU patients characterized by retinal vasculitis treated with conventional therapy (CT, refers to glucocorticoid and immunosuppressive agents) alone or ADA plus conventional therapy with at least 6 months of follow-up between February 2015 and June 2020 were analyzed. Only patients who were first diagnosed with BU without previous systemic treatment were reviewed. The retinal vasculitis score based on fluorescein angiography (FA), best-corrected visual acuity, glucocorticoid-sparing effect, the number of relapses and ocular complications were evaluated. A total of 45 patients (87 eyes) were included. Twenty-four patients (55.33%) in the CT group were treated with conventional therapy and 21 patients (46.67%) in the ADA group were treated with ADA plus conventional therapy. The inflammatory parameters improved in both groups. FA scores showed significantly greater improvement in ADA group than CT group ( < 0.001). The median number of relapses was significantly lower, and the duration of remission was longer in ADA group than CT group ( < 0.001). At the last visit, a significantly better BCVA improvement ( = 0.024), better inflammation control (anterior chamber inflammation = 0.017 and vitritis < 0.001) and lower daily glucocorticoid dosage ( = 0.005) were identified in patients received ADA therapy. In CT group, 1 patient suffered hepatitis B and tuberculosis, 1 had growth retardation, 1 patient had with osteoporosis, then followed by other mild AEs (mostly respiratory upper tract infections); while in ADA group, 1 patient experienced a mild pneumonia ( = 1) while milder AEs were represented mostly by respiratory upper tract infections followed by gastrointestinal discomfort. ADA plus conventional therapy achieved superiority over conventional therapy as initial treatment in naïve BU patients with retinal vasculitis.
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http://dx.doi.org/10.3389/fphar.2021.609148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258106PMC
June 2021

Clinical features and predictors for patients with severe SARS-CoV-2 pneumonia at the start of the pandemic: a retrospective multicenter cohort study.

BMC Infect Dis 2021 Jul 8;21(1):666. Epub 2021 Jul 8.

Department of Cardiology, Ningbo First Hospital, 59 Liuting Road, Ningbo, Zhejiang, China.

Background: This study was performed to investigate clinical features of patients with severe SARS-CoV-2 pneumonia and identify risk factors for converting to severe cases in those who had mild to moderate diseases at the start of the pandemic in China.

Methods: In this retrospective, multicenter cohort study, patients with mild to moderate SARS-CoV-2 pneumonia were included. Demographic data, symptoms, laboratory values, and clinical outcomes were collected. Data were compared between non-severe and severe patients.

Results: 58 patients were included in the final analysis. Compared with non-severe cases, severe patients with SARS-CoV-2 pneumonia had a longer: time to clinical recovery (12·9 ± 4·4 vs 8·3 ± 4·7; P = 0·0011), duration of viral shedding (15·7 ± 6·7 vs 11·8 ± 5·0; P = 0·0183), and hospital stay (20·7 ± 1·2 vs 14·4 ± 4·3; P = 0·0211). Multivariate logistic regression indicated that lymphocyte count was significantly associated with the rate of converting to severe cases (odds ratio 1·28, 95%CI 1·06-1·54, per 0·1 ×  10/L reduced; P = 0·007), while using of low-to-moderate doses of systematic corticosteroids was associated with reduced likelihood of converting to a severe case (odds ratio 0·14, 95%CI 0·02-0·80; P = 0·0275).

Conclusions: The low peripheral blood lymphocyte count was an independent risk factor for SARS-CoV-2 pneumonia patients converting to severe cases. However, this study was carried out right after the start of the pandemic with small sample size. Further prospective studies are warranted to confirm these findings.

Trial Registration: Chinese Clinical Trial Registry, ChiCTR2000029839 . Registered 15 February 2020 - Retrospectively registered.
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http://dx.doi.org/10.1186/s12879-021-06335-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266160PMC
July 2021

Surface functionalization of nanomaterials by aryl diazonium salts for biomedical sciences.

Adv Colloid Interface Sci 2021 Jun 30;294:102479. Epub 2021 Jun 30.

Université de Paris, LCBPT, CNRS, F-75006 Paris, France. Electronic address:

Nanoparticles (NPs) can be prepared by simple reactions and methods from a number of materials. Their small size opens up a number of applications in different fields, among which biomedicine, including: i) drug delivery, ii) biosensors, iii) bioimaging, iv) antibacterial activity. To be able to perform such tasks, NPs must be modified with a variety of functional molecules, such as drugs, targeting groups, chemical tags or antibacterial agents, and must also be prevented from aggregation. The attachment must be stable to resist during the transportation to the targeted location. Diazonium salts, which have been widely used for coupling applications and surface modification, fulfil such criteria. Moreover, they are simple to prepare and can be easily substituted with a large number of organic groups. This review describes the use of these compounds in nanomedicine with a focus on the construction of nanohybrids derived from metal, oxide and carbon-based NPs as well as viruses.
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http://dx.doi.org/10.1016/j.cis.2021.102479DOI Listing
June 2021

Agro-morphological and metabolomics analysis of low nitrogen stress response in .

AoB Plants 2021 Aug 7;13(4):plab022. Epub 2021 May 7.

Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education, College of Forestry, Hainan University, Haikou, Hainan 570228, China.

also known as carpet grass is a robust, stoloniferous grass that can grow in minimal fertilization and resists well to abiotic and biotic stresses including low nitrogen (LN) stress. This study aimed at characterizing the agro-morphological and metabolome responses to LN in carpet grass leaves. Under LN stress, carpet grass increased yellowness of leaves and root dry matter while reduced turf quality and shoot dry weight. The metabolome comparison between samples from optimum and LN conditions indicated 304 differentially accumulated metabolites (DAMs), which could be classified into 12 major and 31 subclasses. The results revealed that the leaf tissues accumulated more anthocyanins and other flavonoid metabolites under LN stress. Conversely, amino acids, nucleic acids and their derivatives were reduced in response to LN stress. The overall evaluation of individual metabolites and pathways, and previous studies on metabolomes indicated that carpet grass reduced its energy consumption in leaves and increased the level of organic acid metabolism and secondary metabolism in order to resist LN stress conditions.
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http://dx.doi.org/10.1093/aobpla/plab022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8256886PMC
August 2021

The Difficulties and Mental Health Intervention Need of Doctors and Nurses in Biological Emergencies: A Qualitative Study.

Front Psychiatry 2021 18;12:696823. Epub 2021 Jun 18.

Department of Pharmacy, Changsha Stomatological Hospital, Changsha, China.

When a biological public health event breaks out, due to the characteristics of their work, doctors and nurses must face risks directly when the situation is unknown. Their difficulties and psychological pressure are unimaginable. However, few studies have investigated the difficulties encountered by those doctors and nurses and their requirements for psychological interventions. This study aimed to explore the difficulties and psychological intervention needs of doctors and nurses during the new biological public health events in China in 2019. We carried out a qualitative study using a phenomenological approach. We used convenience sampling to identify participants who provided direct care and treatment for patients with biological events such as coronavirus disease 2019 (COVID-19). They participated in semi-structured, in-depth face-to-face interviews. The interviews were transcribed and analyzed using Colaizzi's seven-step method. Analysis of this study was divided into the difficulties encountered by doctors and nurses and their mental health need. The difficulties encountered by doctors and nurses included four themes: being worried about the impact on others, lack of knowledge and skills, difficult patients, being socially isolated, and the feeling of uncertainty. The mental health need was summarized into two parts, needs expressed by doctors and nurses and needs observed by researchers. Doctors and nurses mostly did not feel that they needed any psychological support, but the researchers noticed several signs of stress or potential mental health problems among interviewees. Doctors and nurses faced significant complex and multidimensional difficulties. Many denied needing psychological support, even though the researchers noted signs that it might be helpful. Interventions and support strategies that involve mental health promotion activities should consider individual needs related to doctors and nurses' situation.
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http://dx.doi.org/10.3389/fpsyt.2021.696823DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249915PMC
June 2021

FEF% Is a More Sensitive Measure Reflecting Airway Dysfunction in Patients with Asthma: A Comparison Study Using FEF% and FEV.

J Allergy Clin Immunol Pract 2021 Jun 30. Epub 2021 Jun 30.

Department of Allergy and Clinical Immunology, State Key Laboratory of Respiratory Disease, National Clinical Researcher Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, P.R. China. Electronic address:

Background: Reduced forced expiratory flow between 25% and 75% of vital capacity percent predicted (FEF%) representing small airway dysfunction (SAD) was associated with asthma development and progression.

Objective: To investigate whether FEF% was superior to forced expiratory volume in 1 second in predicted (FEV%) in reflecting asthma features in adult patients.

Methods: A retrospective spirometry dataset comprising 1801 adult patients with confirmed asthma and a subgroup of 332 patients having detailed clinical data were used to explore the association of FEF% and/or FEV% with clinical features of asthma.

Results: Of the 1801 subjects, FEV% and FEF% ranged from 136.8% to 10.2% and 127.3% to 3.1%, respectively. FEF% < 65% was present in 1,478 (82.07%) of patients. FEF% was strongly correlated with matched FEV% (r = 0.900, P < .001). FEF% and FEV% were both correlated with airway hyperresponsiveness (r = 0.436, P < .001; r = 0.367, P < .001), asthma control test score (r = 0.329, P < .001; r = 0.335, P < .001), and sputum eosinophil count (r = -0.306, P < .001; r = -0.307, P < .001). Receiver-operating characteristic curves showed that FEF% had greater value in predicting severe asthma (area under the curve: 0.84 vs 0.81, P = .018), airflow obstruction (0.97 vs 0.89, P < .001), and severe bronchial hyperresponsiveness (0.74 vs 0.69, P = .012) as compared with FEV%. Patients with SAD (FEF% < 65%) in the presence of normal FEV% exhibited higher sputum eosinophil counts and had an increased dosage of daily inhaled corticosteroids (P < .001 and P = .010) than patients with normal lung function and their FEF% values correlated with sputum eosinophil count (r = -0.419, P = .015), but not FEV%.

Conclusion: FEF% represented small airway function and was more sensitive at reflecting airway hyperresponsiveness, inflammation, and disease severity as compared with FEV% in patients with asthma. Our data suggest further assessment of FEF% in asthma management, particularly for those with SAD who present normal FEV%.
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http://dx.doi.org/10.1016/j.jaip.2021.06.027DOI Listing
June 2021

Hysteropreservation versus hysterectomy in uterine prolapse surgery: a systematic review and meta-analysis.

Int Urogynecol J 2021 Jul 2. Epub 2021 Jul 2.

Department of Gynecology, Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610014, China.

Introduction And Hypothesis: Hysteropreservation and hysterectomy for uterine prolapse have been compared in several randomized controlled trials (RCTs), as the best treatment has not been definitively determined. This study aimed to summarize the available evidence in RCTs of hysteropreservation versus hysterectomy.

Methods: We performed electronic searches in the PubMed, Embase, Cochrane Library, and China National Knowledge Infrastructure databases for eligible RCTs from inception to June 2020. The relative risks (RRs) and weighted mean differences (WMDs) with corresponding 95% confidence intervals (CIs) were calculated for categorical and continuous variables using random-effects models.

Results: Twelve RCTs involving 1177 patients were selected for meta-analysis. There were no significant differences between hysteropreservation and hysterectomy for the incidences of recurrence (RR, 0.55; 95% CI, 0.26-1.19; P = 0.130) and reoperation (RR, 1.15; 95% CI, 0.56-2.37; P = 0.705). Moreover, neither hysteropreservation nor hysterectomy had any significant effect on the risk of constipation (RR, 0.72; 95% CI, 0.15-3.46; P = 0.681), voiding dysfunction (RR, 0.99; 95% CI, 0.54-1.84; P = 0.981), intraoperative bleeding (RR, 0.35; 95% CI, 0.05-2.26; P = 0.271), upper leg dullness (RR, 0.70; 95% CI, 0.15-3.17; P = 0.643), dyspareunia (RR, 1.47; 95% CI, 0.69-3.13; P = 0.317), and wound infection (RR, 1.38; 95% CI, 0.24-7.80; P = 0.714). Furthermore, hysteropreservation was associated with less intraoperative blood loss (WMD, -25.68; 95% CI, -44.39 to -6.96; P = 0.007), shorter duration of surgery (WMD, -11.30; 95% CI, -19.04 to -3.55; P = 0.004), and shorter duration of hospitalization (WMD, -0.63; 95% CI, -1.10 to -0.16; P = 0.009) compared with hysterectomy.

Conclusion: This study found that both hysteropreservation and hysterectomy have similar effects on recurrence and reoperation rates, while hysteropreservation was superior to hysterectomy in reducing intraoperative blood loss and shortening the duration of surgery and hospitalization.
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http://dx.doi.org/10.1007/s00192-021-04913-9DOI Listing
July 2021
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