Publications by authors named "Li Gong"

500 Publications

Characterization of Insulin-like Peptide (ILP) and Its Potential Role in Ovarian Development of the Cuttlefish .

Curr Issues Mol Biol 2022 May 27;44(6):2490-2504. Epub 2022 May 27.

National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China.

The insulin-like peptide (ILP) family is well known for regulating reproduction in invertebrates, while its role in mollusks remains largely unknown. In this study, we first isolated and characterized the ILP gene in the cuttlefish . The full-length ILP cDNA obtained was 926 bp and encoded a precursor protein of 161 amino acids. The precursor protein consisted of a signal peptide, a B chain, a C-peptide, and an A chain. It possessed the typical features of ILP proteins, including two cleavage sites (KR) and eight conserved cysteines. To define the function of ILP, the expression of ILP in different tissues and ovarian development stages were analyzed using qRT-PCR. was mainly expressed in the ovary, and its gene expression correlated with ovarian development. Furthermore, silencing using RNA interference (RNAi) dramatically decreased the expression levels of four ovarian-development-related genes (vitellogenin1, vitellogenin2, cathepsin L1-like, and follistatin). These data suggest the critical role of in the regulation of ovarian development in .
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http://dx.doi.org/10.3390/cimb44060170DOI Listing
May 2022

PharmGKB summary: acyclovir/ganciclovir pathway.

Pharmacogenet Genomics 2022 Jul 30;32(5):201-208. Epub 2022 May 30.

Departments of Biomedical Data Science.

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http://dx.doi.org/10.1097/FPC.0000000000000474DOI Listing
July 2022

MicroRNA-15a inhibits hepatic stellate cell activation and proliferation via targeting SRY-box transcription factor 9.

Bioengineered 2022 05;13(5):13011-13020

Department of Infectious Diseases, The First People's Hospital of Kunshan, China.

Accumulating research have indicated that microRNAs are associated with the progression of hepatic fibrosis (HF). Nevertheless, the biological role and function of microRNA (miR)-15a in HF are still unknown. Our data revealed that miR-15a expression was decreased in TGF-β1-treated LX-2 cells and CCl-induced mouse model. Additionally, miR-15a could directly target the 3'‑untranslated region of SRY-box transcription factor 9 (SOX9) to inhibit its expression. miR-15a overexpression attenuated the viability and invasion, but enhanced apoptosis in LX-2 cells. However, miR-15a knockdown had the opposite effects. Interestingly, SOX9 overexpression reversed the changes in cell viability, invasion and apoptosis mediated by miR-15a overexpression. Moreover, the miR-15a overexpression-mediated collagen I and alpha smooth muscle actin (a-SMA) downregulation were reversed by SOX9 overexpression. Overall, miR-15a could inhibit LX-2 cell viability and HF pathogenesis by targeting SOX9 in vitro and in vivo.
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http://dx.doi.org/10.1080/21655979.2022.2068895DOI Listing
May 2022

Tislelizumab combined with chemotherapy as neoadjuvant therapy for surgically resectable esophageal cancer: A prospective, single-arm, phase II study (TD-NICE).

Int J Surg 2022 Jul 18;103:106680. Epub 2022 May 18.

Department of Thoracic Surgery, Tangdu Hospital, Air Force Military Medical University, No.1. Xinsi Road, Xi'an, Shaanxi, China. Electronic address:

Background: Clinical benefit of neoadjuvant immunotherapy in resectable esophageal squamous cell carcinoma (ESCC). remains unclear. This study evaluated the efficacy and safety of the programmed death 1 (PD-1) inhibitor tislelizumab combined with chemotherapy as neoadjuvant therapy in patients with resectable ESCC.

Methods: Treatment-naïve patients were enrolled and eligible patients received 3 cycles of neoadjuvant therapy with tislelizumab, carboplatin, and nab-paclitaxel. The primary endpoint was surgery patients major pathological response (MPR). Subgroup analysis was stratified by tumor downstaging, circumferential resection margin (CRM), PD-ligand 1 (PD-L1) expression, and tumor mutation burden (TMB). Safety was assessed by adverse events (AEs) and postoperative complications.

Results: Between September 2020 and March 2021, 45 patients were enrolled. Thirty-six (80.0%) of 45 patients underwent surgery, and 29 (80.5%) underwent successful R0 resection. MPR and pathological complete response (pCR) for surgery patients were 72.0% and 50.0%, respectively. Intention to treatment (ITT) patients MPR and PCR were 57.5% and 40%. Downgrading occurred in 75% of 36 patients. MPR and pCR were identified to be associated with tumor downstaging and CRM but not PD-L1 expression or TMB. TPS levels in MPR and pCR group were significantly higher than that in Non-MPR and Non-pCR group, respectively. Treatment-related AEs of grade 3-4 and immune-related AEs occurred in 42.2% and 22.2% of 45 patients, respectively, and postoperative complications occurred in 77.8% of 36 patients. No treatment-related surgical delay or death occurred. No associations between gene mutation and pathological efficacy were observed.

Conclusions: Tislelizumab plus chemotherapy as neoadjuvant therapy demonstrates promising antitumor activity for resectable ESCC with high rates of MPR, pCR, and R0 resection, as well as acceptable tolerability.
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http://dx.doi.org/10.1016/j.ijsu.2022.106680DOI Listing
July 2022

Antagonistic activity and mechanism of SQ511 against .

3 Biotech 2022 Jun 11;12(6):126. Epub 2022 May 11.

School of Life Sciences and Anhui Key Laboratory of Biodiversity Research and Ecological Protection in Southwest Anhui, Anqing Normal University, 1318 North Jixian Road, Anqing, 246133 Anhui People's Republic of China.

is an important food-borne pathogen. The use of antibiotics is a serious threat to animal and human health, owing to the existence of resistant strains and drug residues. Lactic acid bacteria, as a new alternative to antibiotics, has attracted much attention. In this study, we investigated the antibacterial potential and underlying mechanism of SQ511 against ATCC13076. The results revealed that SQ511 significantly inhibited ATCC13076 growth or even caused death. Laser confocal microscopic imaging revealed that the cell-free supernatant (CFS) of SQ511 elevated the reactive oxygen species level and bacterial membrane depolarization in ATCC13076, leading to cell death. Furthermore, SQ511 CFS had severely deleterious effects on ATCC13076, causing membrane destruction and the release of cellular materials. In addition, SQ511 CFS significantly reduced the expression of virulence, motility, adhesion, and invasion genes in ATCC13076 ( < 0.05), and considerably inhibited motility and biofilm formation capacity ( < 0.05). Thus, antimicrobial compounds produced by SQ511 strongly inhibited growth, mobility, biofilm formation, membrane disruption, and reactive oxygen species generation, and regulated virulence-related gene expressions, presenting promising applications as a probiotic agent.
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http://dx.doi.org/10.1007/s13205-022-03176-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095802PMC
June 2022

A new non-human primate model of desiccating stress-induced dry eye disease.

Sci Rep 2022 May 13;12(1):7957. Epub 2022 May 13.

PriMed Non-Human Primate Research Center of Sichuan PriMed Shines Bio-Tech Co., Ltd., Ya'an, 625000, Sichuan, China.

Dry eye disease (DED), a multifactorial ocular surface disease, is estimated to affect up to 34% of individuals over 50 years old. Although numerous animal models, including rodents and rabbits, have been developed to mimic the pathophysiologic mechanisms involved in dry eye, there is a lack of non-human primate (NHP) models, critical for translational drug studies. Here, we developed a novel desiccating stress-induced dry eye disease model using Rhesus macaque monkeys. The monkeys were housed in a controlled environment room for 21 to 36 days under humidity, temperature, and airflow regulation. Following desiccating stress, NHPs demonstrated clinical symptoms similar to those of humans, as shown by increased corneal fluorescein staining (CFS) and decreased tear-film breakup time (TFBUT). Moreover, corticosteroid treatment significantly reduced CFS scoring, restored TFBUT, and prevented upregulation of tear proinflammatory cytokines as observed in dry eye patients following steroid treatment. The close resemblance of clinical symptoms and treatment responses to those of human DED patients provides great translational value to the NHP model, which could serve as a clinically relevant animal model to study the efficacy of new potential treatments for DED.
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http://dx.doi.org/10.1038/s41598-022-12009-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9106732PMC
May 2022

MRNIP condensates promote DNA double-strand break sensing and end resection.

Nat Commun 2022 May 12;13(1):2638. Epub 2022 May 12.

Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, the Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510655, PR China.

The rapid recognition of DNA double-strand breaks (DSBs) by the MRE11/RAD50/NBS1 (MRN) complex is critical for the initiation of DNA damage response and DSB end resection. Here, we show that MRN complex interacting protein (MRNIP) forms liquid-like condensates to promote homologous recombination-mediated DSB repair. The intrinsically disordered region is essential for MRNIP condensate formation. Mechanically, the MRN complex is compartmentalized and concentrated into MRNIP condensates in the nucleus. After DSB formation, MRNIP condensates move to the damaged DNA rapidly to accelerate the binding of DSB by the concentrated MRN complex, therefore inducing the autophosphorylation of ATM and subsequent activation of DNA damage response signaling. Meanwhile, MRNIP condensates-enhanced MRN complex loading further promotes DSB end resection. In addition, data from xenograft models and clinical samples confirm a correlation between MRNIP and radioresistance. Together, these results reveal an important role of MRNIP phase separation in DSB response and the MRN complex-mediated DSB end resection.
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http://dx.doi.org/10.1038/s41467-022-30303-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098523PMC
May 2022

Primary collision tumors of the sellar region: Experience from a single center.

J Clin Neurosci 2022 Jun 28;100:204-211. Epub 2022 Apr 28.

Department of Pathology, Tangdu Hospital, Air Force Medical University, Xi'an, PR China. Electronic address:

Collision tumors are extremely rare in the sellar region, and their features have not been fully characterized. Here, we report our single-center experience in the diagnosis and management of these tumors, focusing primarily on their clinicopathological features. We first performed a retrospective study of pathological reports from patients who had undergone surgery for pituitary adenoma (PA) or craniopharyngioma (CP) at our hospital. Next, to identify collision tumors, patients with a second pathological diagnosis-such as Rathke's cleft cyst (RCC), gangliocytoma (GC), meningioma, or atypical teratoid/rhabdoid tumor (AT/RT)-were considered. Finally, the clinicopathological characteristics of these tumors were reviewed and analyzed. The results demonstrated that eleven of 2359 PA or CP cases (0.47 %) were found to exhibit sellar collision tumors; the patient cohort had a median age of 52 years (23-71) and was predominantly female (63.6 %, 7/11). In details, of the 2092 cases of PA, 10 were diagnosed with concurrent lesions (seven of RCC and one each of CP, meningioma, and GC). Of the 267 CP cases, a single patient presented with associated AT/RT. To our knowledge, this is the first reported adult case of this subtype. Notably, the preoperative CT and/or MRI of each patient revealed solely PA or CP. The endoscopic endonasal approach was the preferred surgery. In conclusion, the sellar collision tumors occur with low incidence, and the primary subtype is PA and RCC. Their definitive diagnosis depends primarily on pathological findings.
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http://dx.doi.org/10.1016/j.jocn.2022.04.024DOI Listing
June 2022

Can top-down effects of planktivorous fish removal be used to mitigate cyanobacterial blooms in large subtropical highland lakes?

Water Res 2022 Jun 21;218:118483. Epub 2022 Apr 21.

Sino-Danish Centre for Education and Research, Beijing, China; Limnology Laboratory, Department of Biological Sciences, Middle East Technical University, Ankara, Turkey; Centre for Ecosystem Research and Implementation (EKOSAM), Middle East Technical University, Ankara, Turkey; Institute of Marine Sciences, Middle East Technical University, Mersin, Turkey.

Removal of planktivorous fish is used extensively in northern temperate lakes to reduce phytoplankton abundance via enhanced zooplankton grazing. However, whether this method would work also in large subtropical highland lakes to alleviate cyanobacterial blooms is unknown. We conducted a one-year pilot in situ experiment where we removed a substantial biomass of fish in a fenced-in area, followed by a 3-year whole-lake experiment where the dominant fish species (Japanese smelt) was removed in Lake Erhai in southwest China. The fencing experiments showed that between July and November, when the biomass of the removed stock reached 4 g/m, the zooplankton biomass inside the fence increased significantly compared to a control fence. In the full-lake experiment, we found that sustained removal of Japanese smelt led to an increase in the biomass of cladocerans (Daphnia spp. but especially of Bosmina spp.) and a significant decrease in the biomass of Cyanobacteria and Chlorophyta. Additionally, a marked increase in the ratio of zooplankton to phytoplankton biomass, as well as an increase in the body size of cladocerans, emphasising the importance of enhanced top-down control for mitigating cyanobacterial blooms following extensive fish removal. Our results reveal that removal of small fish (here Japanese smelt) can lead to a reduction of the phytoplankton and cyanobacteria biomass through a trophic cascade in highland deep subtropical lakes. Thus fish removal may be a feasible additional restoration tool to external nutrient loading reduction in such lakes.
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http://dx.doi.org/10.1016/j.watres.2022.118483DOI Listing
June 2022

Chromium-Catalyzed Selective Borylation of Vinyl Triflates and Unactivated Aryl Carboxylic Esters with Pinacolborane.

Org Lett 2022 May 25;24(17):3227-3231. Epub 2022 Apr 25.

Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China.

The use of pinacolborane to borylate abundant vinyl triflates and unactivated aryl carboxylic esters was enabled by chromium catalysis via the selective formation of vinyl and aryl boronate esters. The competing hydrided reduction or allylic borylation proceeds sluggishly or does not occur, therefore providing a selective strategy for the incorporation of boronate into olefins and arenes. Mechanistic studies indicate that the σ-bond metathesis or oxidative addition mechanism may be considered to be responsible for the cleavage of ester scaffolds.
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http://dx.doi.org/10.1021/acs.orglett.2c01015DOI Listing
May 2022

Chinese expert consensus on conversion therapy for hepatocellular carcinoma (2021 edition).

Hepatobiliary Surg Nutr 2022 Apr;11(2):227-252

Division of General Surgery, First Affiliated Hospital, Anhui Medical University, Hefei, China.

Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma (HCC) have resulted in improved response rates. This has provided an opportunity for selected patients with initially unresectable HCC to achieve adequate tumor downstaging to undergo surgical resection, a 'conversion therapy' strategy. However, conversion therapy is a new approach to the treatment of HCC and its practice and treatment protocols are still being developed. Review the evidence for conversion therapy in HCC and develop consensus statements to guide clinical practice. Evidence review: Many research centers in China have accumulated significant experience implementing HCC conversion therapy. Preliminary findings and data have shown that conversion therapy represents an important strategy to maximize the survival of selected patients with intermediate stage to advanced HCC; however, there are still many urgent clinical and scientific challenges for this therapeutic strategy and its related fields. In order to summarize and learn from past experience and review current challenges, the Chinese Expert Consensus on Conversion Therapy for Hepatocellular Carcinoma (2021 Edition) was developed based on a review of preliminary experience and clinical data from Chinese and non-Chinese studies in this field and combined with recommendations for clinical practice. Sixteen consensus statements on the implementation of conversion therapy for HCC were developed. The statements generated in this review are based on a review of clinical evidence and real clinical experience and will help guide future progress in conversion therapy for patients with HCC.
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http://dx.doi.org/10.21037/hbsn-21-328DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9023831PMC
April 2022

Identification, expression and functional analysis of activin type I receptor in common Chinese Cuttlefish, Sepiella japonica.

Anim Reprod Sci 2022 May 11;240:106976. Epub 2022 Apr 11.

Zhejiang Key Laboratory of Aquatic Germplasm Resources, Zhejiang Wanli University, Ningbo, China. Electronic address:

Activin, a member of the transforming growth factor-β(TGF-β) superfamily, exerts its actions by binding to specific transmembrane serine/threonine kinase receptors, known as types II and I receptors. In this study, a full-length cDNA encoding for activin type Ⅰ receptor (SjActRⅠ) was cloned, characterized, and functionally studied in S.japonica. The full-length cDNA of SjActRⅠwas 2264 bp and encoded 505 amino acids. Subcellular localization analysis showed that SjActRⅠwas distributed in the plasma membrane in HEK293T cells. qRT-PCR showed that expressions of SjActRⅠ were ubiquitously expressed in all examined tissues, with the highest expression in the ovary. During the different ovarian development, the expression levels of SjActRⅠ in these three tissues (ovary, brain, and liver) increased from stage I to stage Ⅲ and then decreased from stage III to stage IV. Injection of exogenous GnRH significantly increased the mRNA levels of SjActRⅠ in the ovary and liver, while the expression level of sjActRI in the brain showed no significant difference. Knock-down of SjActRⅠ, by injection of double-strand RNA (dsRNA) significantly reduced the expression levels of SjActRⅠ. Furthermore, silencing SjActRⅠsignificantly reduced the expression of Vitellogenin in the ovary, suggesting that SjActRⅠ might promote ovarian development by stimulating the expression of Vitellogenin. These findings implied that SjActRⅠ might play a crucial role in ovarian development in S.japonica. In conclusion, our results provide novel insights into the evolution and roles of the ActRⅠgene in cuttlefish.
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http://dx.doi.org/10.1016/j.anireprosci.2022.106976DOI Listing
May 2022

Ground Glass Opacity and Adjuvant Chemotherapy in Pathological Stage IB-IIA Lung Adenocarcinoma.

Front Oncol 2022 25;12:851276. Epub 2022 Mar 25.

Department of Thoracic Surgery, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Background: The prognostic value of ground glass opacity (GGO) in stage IA non-small cell lung cancer (NSCLC) has been widely recognized. However, studies investigating its value in the related stage IB-IIA lung adenocarcinoma (LUAD) remains lacking. The impact of adjuvant chemotherapy (ACT) on pathological stage IB-IIA LUAD is also controversial.

Materials And Methods: We retrospectively reviewed the clinical records of 501 patients with pathological stage IB-IIA LUAD at the Sun Yat-sen University Cancer Center from January 2008 to June 2018. We calculated and compared survival curves using the Kaplan-Meier test and log-rank test. Cox regression models were performed to determine independent prognostic factors of disease-free survival (DFS) and overall survival (OS). We established nomograms to predict the OS and DFS of LUAD patients. Calibration and receiver operator characteristic curves were conducted to assess the predictive performance of two nomograms. Based on the nomogram, we identified candidate patients that may most benefit from ACT after surgery.

Results: The number of patients with pure solid, part GGO, and pure GGO nodules was 240, 242, and 19, respectively, and 125 patients who received ACT. Patients with consolidation-to-tumor ratio (CTR) <0.75 had longer OS ( = 0.026) and DFS ( = 0.003). Pathological tumor size and at least 10 lymph nodes (LNs) resection were independent prognostic factors of both OS and DFS. CTR <0.75 was positively associated with DFS. The C-index of nomograms predicting individual OS and DFS was 0.660 and 0.634, respectively. Based on the nomogram for OS, ACT was found to be a positive prognostic indicator of OS ( = 0.031, HR = 0.5141, 95% CI 0.281-0.942) in patients with nomogram total points ≥5.

Conclusion: CTR <0.75 is associated with a better DFS in patients with stage IB-IIA LUAD. Nomograms developed by integrating pathological tumor size, at least 10 LNs resection, and CTR ≥0.75 for predicting individual OS and DFS displayed a good predictive capacity and clinical value, which were also proved to be a useful tool for selecting patients most benefiting from ACT.
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http://dx.doi.org/10.3389/fonc.2022.851276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8990754PMC
March 2022

Neurofilament Light Chain (NF-L) Stimulates Lipid Peroxidation to Neuronal Membrane through Microglia-Derived Ferritin Heavy Chain (FTH) Secretion.

Oxid Med Cell Longev 2022 24;2022:3938940. Epub 2022 Mar 24.

Department of Neurology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.

A part of the axonal cytoskeleton protein complex, neurofilament light chain (NF-L) has been suggested as a pathological hallmark in various neurological disorders, including hemorrhagic stroke, vascular dementia, and cerebral small vessel disease. Neuroaxonal debris are mainly engulfed and phagocytosed by microglia, while the effects of NF-L on microglia have not been elucidated. Ferritin heavy chain (FTH) not only reflects the age-related status of microglia but may also be secreted into the extracellular space. After treatment of microglia with varying concentrations of NF-L (0-3 g/ml), we found robust increases in the number of secretory FTH-containing exosomes in the medium. Induction of the FTH-containing exosomes secreted from microglia stimulates neuronal loss and membrane lipid peroxidation, as assessed by CKK8 and C11-Bodipy, respectively. However, this oxidative stress damage was attenuated by blocking expression. Our results suggest that NF-L, as a biomarker of axonal injury itself, could participate in neuronal ferroptosis in a nonclassical manner by secreting FTH-containing exosomes from microglia into the extracellular matrix.
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http://dx.doi.org/10.1155/2022/3938940DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8972155PMC
April 2022

Identification of a novel heterozygous SOX9 variant in a Chinese family with congenital heart disease.

Mol Genet Genomic Med 2022 05 26;10(5):e1909. Epub 2022 Feb 26.

Department of Medical Genetics and Developmental Biology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.

Background: Previous studies of individuals with hereditary or sporadic congenital heart disease (CHD) have provided strong evidence for a genetic basis for CHD. The aim of this study was to identify novel pathogenic genes and variants in a Chinese CHD family.

Methods: Three generations of a family with CHD were recruited. We performed whole exome sequencing for the affected individuals and the proband's unaffected aunt to investigate the genetic causes of CHD in this family. Heterozygous variants carried by the proband and her maternal grandmother, but not the proband's aunt, were selected. The frequencies of the variants detected were assessed using public databases, and their influences on protein function were predicted using online prediction software. The candidate variant was further confirmed by Sanger sequencing of other members of the family.

Results: On the basis of the family's pedigree, the mode of inheritance was speculated to be autosomal dominant with incomplete penetrance. We identified a novel heterozygous missense variant in SOX9 in all affected individuals and one asymptomatic family member, suggesting an inheritance pattern with incomplete penetrance. The variant was not found in any public database. In addition, the variant was highly conserved among mammals, and was predicted to be deleterious by online software programs.

Conclusions: We report for the first time a novel heterozygous missense variant in SOX9 (NM_000346:c.931G>T:p.Gly311Cys) in a Chinese CHD family. Our results provide further evidence supporting a causative role for SOX9 variants in CHD.
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http://dx.doi.org/10.1002/mgg3.1909DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034670PMC
May 2022

Probiotic Alleviates Oxidative Stress-Induced Liver Injury by Modulating Gut-Liver Axis in a Rat Model.

Antioxidants (Basel) 2022 Jan 31;11(2). Epub 2022 Jan 31.

Key Laboratory of Molecular Animal Nutrition of the Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

Emerging evidence suggests a key role of gut microbiota in maintaining liver functions through modulating the gut-liver axis. In this study, we investigated whether microbiota alteration mediated by probiotic was involved in alleviating oxidative stress- induced liver injury. Sprague-Dawley rats were orally administered SC06 or SC08 for a 24-day period and thereafter intraperitoneally injected diquat (DQ) to induce oxidative stress. Results showed that , particularly SC06 significantly inhibited hepatic injuries, as evidenced by the alleviated damaged liver structure, the decreased levels of ALT, AST, ALP and LDH, and the suppressed mitochondrial dysfunction. SC06 pretreatment markedly enhanced the liver antioxidant capacity by decreasing MDA and p47, and increasing T-AOC, SOD and HO-1.16S rRNA sequencing analysis revealed that DQ significantly changed the diversities and composition of gut microbiota, whereas pretreatments could attenuate gut dysbiosis. Pearson's correlation analysis showed that AST and MDA exerted a positive correlation with the opportunistic pathogenic genera and species ( and ), and negatively correlated with the potential probiotics (), while SOD exerted a reverse trend. The microbial metagenomic analysis demonstrated that , particularly SC06 markedly suppress the metabolic pathways such as carbohydrate metabolism, lipid metabolism, amino acid metabolism and metabolism of cofactors and vitamins. Furthermore, SC06 decreased the gene abundance of the pathways mediating bacterial replication, secretion and pathogenicity. Taken together, SC06 alleviates oxidative stress-induced liver injuries via optimizing the composition, metabolic pathways and pathogenic replication and secretion of gut microbiota. These findings elucidate the mechanisms of probiotics in alleviating oxidative stress and provide a promising strategy for preventing liver diseases by targeting gut microbiota.
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http://dx.doi.org/10.3390/antiox11020291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8868294PMC
January 2022

The Clinical Pharmacogenetics Implementation Consortium Guideline for SLCO1B1, ABCG2, and CYP2C9 genotypes and Statin-Associated Musculoskeletal Symptoms.

Clin Pharmacol Ther 2022 05 11;111(5):1007-1021. Epub 2022 Mar 11.

Department of Medicine, Duke Center for Applied Genomics & Precision Medicine, Duke University School of Medicine, Durham, North Carolina, USA.

Statins reduce cholesterol, prevent cardiovascular disease, and are among the most commonly prescribed medications in the world. Statin-associated musculoskeletal symptoms (SAMS) impact statin adherence and ultimately can impede the long-term effectiveness of statin therapy. There are several identified pharmacogenetic variants that impact statin disposition and adverse events during statin therapy. SLCO1B1 encodes a transporter (SLCO1B1; alternative names include OATP1B1 or OATP-C) that facilitates the hepatic uptake of all statins. ABCG2 encodes an efflux transporter (BCRP) that modulates the absorption and disposition of rosuvastatin. CYP2C9 encodes a phase I drug metabolizing enzyme responsible for the oxidation of some statins. Genetic variation in each of these genes alters systemic exposure to statins (i.e., simvastatin, rosuvastatin, pravastatin, pitavastatin, atorvastatin, fluvastatin, lovastatin), which can increase the risk for SAMS. We summarize the literature supporting these associations and provide therapeutic recommendations for statins based on SLCO1B1, ABCG2, and CYP2C9 genotype with the goal of improving the overall safety, adherence, and effectiveness of statin therapy. This document replaces the 2012 and 2014 Clinical Pharmacogenetics Implementation Consortium (CPIC) guidelines for SLCO1B1 and simvastatin-induced myopathy.
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http://dx.doi.org/10.1002/cpt.2557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035072PMC
May 2022

Oriented Growth of Thin Films of Covalent Organic Frameworks with Large Single-Crystalline Domains on the Water Surface.

J Am Chem Soc 2022 Feb 11;144(7):3233-3241. Epub 2022 Feb 11.

Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, School of Chemistry, and State Key Laboratory of Optoelectronic Materials and Technologies, Sun Yat-sen University, 510275 Guangzhou, Guangdong, China.

It has been a longstanding challenge to rationally synthesize thin films of organic two-dimensional (2D) crystals with large single-crystalline domains. Here, we present a general strategy for the creation of 2D crystals of covalent organic frameworks (COFs) on the water surface, assisted by a charged polymer. The morphology of the preorganized monomers underneath the charged polymer on the water surface and their diffusion were crucial for the formation of the organic 2D crystals. Thin films of 2D COFs with an average single-crystalline domain size of around 3.57 ± 2.57 μm have been achieved, and their lattice structure, molecular structure, and grain boundaries were identified with a resolution down to 3 Å. The swing of chain segments and lattice distortion were revealed as key factors in compensating for the misorientation between adjacent grains and facilitating error corrections at the grain boundaries, giving rise to larger single-crystalline domains. The generality of the synthesis method was further proved with three additional 2D COFs. The oriented single-crystalline domains and clear grain boundaries render the films as model materials to study the dependence of the vertical conductivity of organic 2D crystals on domain sizes and chemical structures, and significant grain boundary effects were illustrated. This study presents a breakthrough in the controlled synthesis of organic 2D crystals with structural control at the molecular level. We envisage that this work will inspire further investigation into the microstructure-intrinsic property correlation of 2D COFs and boost their application in electronics.
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http://dx.doi.org/10.1021/jacs.1c13195DOI Listing
February 2022

Chromosome-level genome assembly of the dotted gizzard shad ( ) provides insights into its adaptive evolution.

Zool Res 2022 03;43(2):217-220

National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.

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http://dx.doi.org/10.24272/j.issn.2095-8137.2021.351DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8920852PMC
March 2022

PD-L1 expression and immune cells infiltration in primary tracheobronchial neoplasm.

Transl Lung Cancer Res 2021 Dec;10(12):4617-4630

Department of Thoracic Surgery, Tangdu Hospital, Airforce Military Medical University, Xi'an, China.

Background: Primary tracheobronchial neoplasm is rare yet poses a serious threat to life. Due to its low incidence, the immune microenvironment of such tumors remained unclear. This study aimed to clarify the expression of programmed death-ligand 1 (PD-L1) and infiltration of immune cells in primary tracheobronchial neoplasm, which might be useful for guiding treatment and evaluating clinical outcome.

Methods: We assessed retrospectively the expression of PD-L1 and infiltration in cells expressing CD8, CD16, CD68, CD163 and FOXP3 in 21 patients with primary tracheobronchial neoplasm who underwent surgery in Tangdu Hospital from January 2016 to July 2021. The expression of PD-L1 was assessed based on the tumor proportion score system. The density of immune cells was analyzed by automatic image analysis software.

Results: In this study, all of 16 participants with adenoid cystic carcinoma (ACC) had no expression of PD-L1, whereas 4/5 (80%) of those with squamous cell carcinomas (SCC) were positive for PD-L1 expression. Compared with ACC, the density of FOXP3 cells in both the intratumoral region and peritumoral region was higher in SCC (P<0.01). The density of FOXP3 cells was significantly higher than that of CD8, CD16, and CD163 cells in SCC in the intratumoral region (P<0.01). In contrast, the density of FOXP3 cells was significantly lower than that of CD8, CD16, and CD68 cells in ACC in both the intratumoral region and peritumoral regions. The density of CD68 cells was significantly higher than that of CD8 cells (P<0.05) and CD163 cells (P<0.01) in ACC in the intratumoral region. Furthermore, the tumors of patients with metastasis more commonly of immune-excluded status, in which the CD8 cells accumulated in peritumoral region.

Conclusions: This study demonstrated that the expression of PD-L1 in primary tracheobronchial neoplasm was mainly concentrated in patients with SCC. In the immune microenvironment of SCC, FOXP3 cells were the dominant immune cells, while in the immune microenvironment of ACC, CD68 cells were the main immune cells. Therefore, the immune microenvironment was significantly different in primary tracheobronchial neoplasm according to histology.
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http://dx.doi.org/10.21037/tlcr-21-958DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743529PMC
December 2021

Unclonable Photonic Crystal Hydrogels with Controllable Encoding Capacity for Anticounterfeiting.

ACS Appl Mater Interfaces 2022 Jan 27;14(1):2369-2380. Epub 2021 Dec 27.

Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, School of Chemistry, Sun Yat-sen University, Guangzhou 510275, P. R. China.

Inspired by the formation of random sparkling microcrystallines in naturally precious opals, we develop a new strategy to produce a class of unclonable photonic crystal hydrogels (UPCHs) induced by the electrostatic interaction effect, which further achieve unclonable encoding/decoding and random high-encrypted patterns along with an ultrahigh and controllable encoding capacity up to ca. 2 × 10. Owing to the randomness of colloidal crystals in the self-assembly process, UPCHs with randomly distributed sparkling spots are endowed with unpredictable/unrepeatable characteristics. This, coupled with the water response of UPCHs with angle dependence and robustness, can upgrade the encryption level and address some limitations of easy fading, limited durability, and high cost in practical uses of existing unclonable materials. Interestingly, UPCHs can be readily patterned to exhibit reliable and rapid authentication by utilizing artificial intelligence (AI) deep learning, which can find broad applications in developing unbreakable and portable information storage/steganography systems not limited to anticounterfeiting.
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http://dx.doi.org/10.1021/acsami.1c20905DOI Listing
January 2022

Prognostic Nomograms Based on Ground Glass Opacity and Subtype of Lung Adenocarcinoma for Patients with Pathological Stage IA Lung Adenocarcinoma.

Front Cell Dev Biol 2021 8;9:769881. Epub 2021 Dec 8.

Department of Thoracic Surgery, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

The value of lung adenocarcinoma (LUAD) subtypes and ground glass opacity (GGO) in pathological stage IA invasive adenocarcinoma (IAC) has been poorly understood, and reports of their association with each other have been limited. In the current study, we retrospectively reviewed 484 patients with pathological stage IA invasive adenocarcinoma (IAC) at Sun Yat-sen University Cancer Center from March 2011 to August 2018. Patients with at least 5% solid or micropapillary presence were categorized as high-risk subtypes. Independent indicators for disease-free survival (DFS) and overall survival (OS) were identified by multivariate Cox regression analysis. Based on these indicators, we developed prognostic nomograms of OS and DFS. The predictive performance of the two nomograms were assessed by calibration plots. A total of 412 patients were recognized as having the low-risk subtype, and 359 patients had a GGO. Patients with the low-risk subtype had a high rate of GGO nodules ( < 0.001). Multivariate Cox regression analysis showed that the high-risk subtype and GGO components were independent prognostic factors for OS (LUAD subtype: = 0.002; HR 3.624; 95% CI 1.263-10.397; GGO component: = 0.001; HR 3.186; 95% CI 1.155-8.792) and DFS (LUAD subtype: = 0.001; HR 2.284; 95% CI 1.448-5.509; GGO component: = 0.003; HR 1.877; 95% CI 1.013-3.476). The C-indices of the nomogram based on the LUAD subtype and GGO components to predict OS and DFS were 0.866 (95% CI 0.841-0.891) and 0.667 (95% CI 0.5860.748), respectively. Therefore, the high-risk subtype and GGO components were potential prognostic biomarkers for patients with stage IA IAC, and prognostic models based on these indicators showed good predictive performance and satisfactory agreement between observational and predicted survival.
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http://dx.doi.org/10.3389/fcell.2021.769881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8692790PMC
December 2021

Compound Kushen Injection Protects Skin From Radiation Injury Regulating Bim.

Front Pharmacol 2021 9;12:753068. Epub 2021 Dec 9.

Department of Oncology, Clinical and Basic Research Team of TCM Prevention and Treatment of NSCLC, the Second Clinical College of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, Guangdong-Hong Kong-Macau Joint Lab on Chinese Medicine and Immune Disease Research, Guangzhou University of Chinese Medicine, Guangzhou, China.

Radiation-induced skin injury is a major side-effect observed in cancer patients who received radiotherapy. Thus identifying new radioprotective drugs for prevention or treatment of post-irradiation skin injury should be prompted. A large number of clinical studies have confirmed that Compound Kushen injection (CKI) can enhance efficacy and reduce toxicity of radiotherapy. The aim of this study is to confirm the effect of CKI in alleviating radiotherapy injury in the skin and explore the exact mechanism. 60 patients who met the inclusion/exclusion criteria were allocated to treatment group (CKI before radiotherapy) or control group (normal saline before radiotherapy) randomly. MTT assay, flow cytometry, Western Blot, and transient transfection were performed to detect the cell viability, cell apoptosis and Bim expression after treatment with CKI or/and radiotherapy. CKI had the effect of alleviating skin injury in cancer patients who received radiotherapy in clinic. CKI induced cancer cell apoptosis when combined with irradiation (IR), while it reversed the induction of cell apoptosis by IR in human skin fibroblast (HSF) cells. And Bim, as a tumor suppressor, was induced in cancer cells but had no change in HSF cells when treated with CKI. Moreover, the above effect could be attenuated when Bim was silenced by siRNA. We conclude that CKI represents a promising radio-protective agent with a potential differential beneficial effect on both cancer cells (inducing apoptosis) and HSF cells (providing radio-protection inhibiting IR-induced apoptosis), via regulating Bim. Our study uncovers a novel mechanism by which CKI inhibits human cancer cell while protects skin from radiotherapy, indicating CKI might be a promising radio-protective drug. Chinese Clinical Trial Registry (www.chictr.org.cn), identifier ChiCTR2100049164.
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http://dx.doi.org/10.3389/fphar.2021.753068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8696473PMC
December 2021

Effects of Dietary Tributyrin on Growth Performance, Biochemical Indices, and Intestinal Microbiota of Yellow-Feathered Broilers.

Animals (Basel) 2021 Dec 1;11(12). Epub 2021 Dec 1.

School of Life Science and Engineering, Foshan University, Foshan 528000, China.

This study aimed to evaluate the effects of tributyrin on growth performance, biochemical indices and intestinal microbiota of yellow-feathered broilers. 360 one-day-old chicks were randomly allocated to three treatments with six replicates of 20 chicks each, including a normal control group (NC), an antibiotic group (PC), and a tributyrin (250 mg/kg) group (TB) for 63 days. The results showed that compared with the control, the feed conversion ratio (FCR) in the TB group decreased during the d22 to d42 ( < 0.05) and overall, the final weight and FCR of broilers tended to increase and decrease, respectively. Moreover, the TB group showed the highest creatine concentrations at the entire period ( < 0.05). TB treatment increased the relative abundance and decreased . Principal coordinates analysis yielded clear clustering of the three groups. Linear discriminant analysis effect size analysis found seven differentially abundant taxa in the TB group, including several members of . The relative abundance of , , and increased in tributyrin-treated broilers. Spearman correlation analysis identified a correlation between abundance and overall feed efficiency. These results demonstrated that tributyrin could improve the growth performance by modulating blood biochemical indices and the cecal microflora composition of broilers.
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http://dx.doi.org/10.3390/ani11123425DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8697914PMC
December 2021

A durable superhydrophobic porous polymer coated sponge for efficient separation of immiscible oil/water mixtures and oil-in-water emulsions.

J Hazard Mater 2022 Mar 3;425:127980. Epub 2021 Dec 3.

Department of Environmental Science, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Environmental Pollution and Ecological Health of Ministry of Education, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Hangzhou 310058, China; Zhejiang University-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou 311200, China. Electronic address:

Oil spills and organic solvents leakages have led to serious environmental problems, which calls for the emerging materials for the separation of oil/organic solvents from water effectively. Herein, a superhydrophobic/superoleophilic, [email protected], with water contact angle (WCA) of 156° and oil contact angle of 0°, was fabricated by dip coating polymer SHMP-1 powder onto the skeleton of 3D melamine sponge. The [email protected] featured large specific surface area (556 m/g) as well as high chemical and mechanical durability. [email protected] can absorb up to 40-105 times of its own weight of light and heavy oils/organic solvents in seconds, and it can be recycled for 25 times by squeezing. Moreover, the separation efficiency of immiscible oil/water mixtures and oil-in-water emulsions by [email protected] are > 99.5%. [email protected] shows tremendous absorption capacity for chloroform-in-water emulsions (1460 mg/g) compared with nitrobenzene-in-water (1290 mg/g) and diesel-in-water emulsions (980 mg/g), which is the strongest superhydrophobic absorbent for surfactant-stabilized oil-in-water emulsions reported to date. The durable [email protected] fabricated by loading superhydrophobic polymer with large surface area onto 3D sponge makes it a promising material for oil/water separation in realistic aquatic environments.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127980DOI Listing
March 2022

Editorial: NSAIDs Pharmacogenomics.

Front Pharmacol 2021 19;12:798447. Epub 2021 Nov 19.

University of Pennsylvania, Philadelphia, PA, United States.

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http://dx.doi.org/10.3389/fphar.2021.798447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8640121PMC
November 2021

Protective Effects of Lac16 on Infection-Associated Injury in IPEC-J2 Cells.

Int J Mol Sci 2021 Nov 17;22(22). Epub 2021 Nov 17.

Key Laboratory of Molecular Animal Nutrition of the Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science (Eastern of China) of the Ministry of Agriculture, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Institute of Animal Nutrition and Feed Sciences, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

() causes intestinal injury through overgrowth and the secretion of multiple toxins, leading to diarrhea and necrotic enteritis in animals, including pigs, chickens, and sheep. This study aimed to investigate the protective effects of () Lac16 on infection-associated injury in intestinal porcine epithelial cell line (IPEC-J2). The results showed that Lac16 significantly inhibited the growth of , which was accompanied by a decrease in pH levels. In addition, Lac16 significantly elevated the mRNA expression levels of host defense peptides (HDPs) in IPEC-J2 cells, decreased the adhesion of to IPEC-J2 cells, and attenuated -induced cellular cytotoxicity and intestinal barrier damage. Furthermore, Lac16 significantly suppressed -induced gene expressions of proinflammatory cytokines and pattern recognition receptors (PRRs) in IPEC-J2 cells. Moreover, Lac16 preincubation effectively inhibited the phosphorylation of p65 caused by infection. Collectively, probiotic Lac16 exerts protective effects against infection-associated injury in IPEC-J2 cells.
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http://dx.doi.org/10.3390/ijms222212388DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8620398PMC
November 2021

Coincorporation of N and S into Zero-Valent Iron to Enhance TCE Dechlorination: Kinetics, Electron Efficiency, and Dechlorination Capacity.

Environ Sci Technol 2021 12 17;55(23):16088-16098. Epub 2021 Nov 17.

College of Environment, Zhejiang University of Technology, Hangzhou 310014, China.

Sulfidated zero-valent iron (S-ZVI) enhances the degradation of chlorinated hydrocarbon (CHC) in contaminated groundwater. Despite numerous studies of S-ZVI, a versatile strategy to improve its dechlorination kinetics, electron efficiency (ε), and dechlorination capacity is still needed. Here, we used heteroatom incorporation of N(C) and S by ball-milling of microscale ZVI with melamine and sulfur via nitridation and sulfidation to synthesize S-N(C)-mZVI particles that contain reactive Fe-N(C) and FeS species. Sulfidation and nitridation synergistically increased the trichloroethene (TCE) dechlorination rate, with reaction constants of 2.98 × 10 L·h·m by S-N(C)-mZVI, compared to 1.77 × 10 and 8.15 × 10 L·h·m by S-mZVI and N(C)-mZVI, respectively. Data show that sulfidation suppressed the reductive dissociation of N(C) from S-N(C)-mZVI, which stabilized the reactive Fe-N(C) and reserved electrons for TCE dechlorination. In addition to lowering H production, S-N(C)-mZVI dechlorinated TCE to less reduced products (e.g., acetylene), contributing to the material's higher ε and dechlorination capacity. This synergistic effect on TCE degradation can be extended to other recalcitrant CHCs (e.g., chloroform) in both deionized and groundwater. This multiheteroatom incorporation approach to optimize ZVI for groundwater remediation provides a basis for further advances in reactive material synthesis.
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http://dx.doi.org/10.1021/acs.est.1c03784DOI Listing
December 2021

Different gene rearrangements of the genus Dardanus (Anomura: Diogenidae) and insights into the phylogeny of Paguroidea.

Sci Rep 2021 11 8;11(1):21833. Epub 2021 Nov 8.

National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, 316022, China.

Complete mitochondrial genomes (mitogenomes) can provide useful information for phylogenetic relationships, gene rearrangement, and molecular evolution. In this study, the complete mitogenomes of two hermit crabs, Dardanus arrosor and Dardanus aspersus, were sequenced for the first time and compared with other published mitogenomes of Paguroidea. Each of the two mitogenomes contains an entire set of 37 genes and a putative control region, but they display different gene arrangements. The different arrangements of the two mitogenomes might be the result of transposition, reversal, and tandem duplication/random loss events from the ancestral pancrustacean pattern. Genome sequence similarity analysis reveals the gene rearrangement in 15 Paguroidea mitogenomes. After synteny analysis between the 15 Paguroidea mitogenomes, an obvious rearranged region is found in D. aspersus mitogenome. Across the 13 protein-coding genes (PCGs) tested, COI has the least and ND6 has the largest genetic distances among the 15 hermit crabs, indicating varied evolution rates of PCGs. In addition, the dN/dS ratio analysis shows that all PCGs are evolving under purifying selection. The phylogenetic analyses based on both gene order and sequence data present the monophyly of three families (Paguridae, Coenobitidae, and Pylochelidae) and the paraphyly of the family Diogenidae. Meanwhile, the phylogenetic tree based on the nucleotide sequences of 13 PCGs shows that two Dardanus species formed a sister group with five Coenobitidae species. These findings help to better understand the gene rearrangement and phylogeny of Paguroidea, as well as provide new insights into the usefulness of mitochondrial gene order as a phylogenetic marker.
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http://dx.doi.org/10.1038/s41598-021-01338-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8576005PMC
November 2021

Dietary Supplementation With Ameliorates Compromise of Growth Performance by Modulating Short-Chain Fatty Acids and Intestinal Dysbiosis in Broilers Under Challenge.

Front Nutr 2021 14;8:706148. Epub 2021 Oct 14.

Key Laboratory of Molecular Animal Nutrition of the Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science (Eastern of China) of the Ministry of Agriculture, Key Laboratory of Animal Feed and Nutrition of Zhejiang, College of Animal Sciences, Institute of Animal Nutrition and Feed Sciences, Zhejiang University, Hangzhou, China.

is an important zoonotic pathogen associated with food contamination and poisoning, gas gangrene, necrotizing enterocolitis or necrotic enteritis in humans and animals. Dysbacteriosis is supposedly associated with the development of infection induced necrotic enteritis, but the detailed relationship between intestinal health, microbiome, and infection-induced necrotic enteritis remains poorly understood. This research investigated the effect of probiotics on the growth performance and intestinal health of broilers, and the involved roles of intestinal microbiota and microbial metabolic functions under infection. Results showed that subclinical necrotic enteritis was successfully induced as evidenced by the significant lower body weight (BW), suppressed feed conversion ratio (FCR), decreased ileal villus height and mucosal barrier function, and increased ileal histopathological score and bursal weight index. or significantly attenuated -induced compromise of growth performance (BW, FCR) and ileal mucosa damage as illustrated by the increased ileal villus height and villus/crypt ratio, the decreased ileal histopathological score and the enhanced ileal mucosal barrier function. also significantly alleviated -induced enlarged bursa of fabricius and the decreased levels of ileal total SCFAs, acetate, lactate, and butyrate. Furthermore, dietary improved infection-induced intestinal dysbiosis as evidenced by significantly enriched short-chain fatty acids-producing bacteria (), reduced drug-resistant bacteria () and enteric pathogens () and bacterial metabolic dysfunctions as illustrated by significantly increased bacterial fatty acid biosynthesis, decreased bacterial lipopolysaccharide biosynthesis, and antibiotic biosynthesis (streptomycin and vancomycin). Additionally, the BW and intestinal SCFAs were the principal factors affecting the bacterial communities and microbial metabolic functions. The above findings indicate that dietary with attenuates -induced compromise of growth performance and intestinal dysbiosis by increasing SCFAs and improving intestinal health in broilers.
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http://dx.doi.org/10.3389/fnut.2021.706148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551491PMC
October 2021
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