Publications by authors named "Li Gao"

1,673 Publications

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Automatic arteriosclerotic retinopathy grading using four-channel with image merging.

Comput Methods Programs Biomed 2021 Jul 21;208:106274. Epub 2021 Jul 21.

College of Computer Science, Nankai University, Tianjin, China. Electronic address:

Background And Objective: Arteriosclerosis can reflect the severity of hypertension, which is one of the main diseases threatening human life safety. But Arteriosclerosis retinopathy detection involves costly and time-consuming manual assessment. To meet the urgent needs of automation, this paper developed a novel arteriosclerosis retinopathy grading method based on convolutional neural network.

Methods: Firstly, we propose a good scheme for extracting features facing the fundus blood vessel background using image merging for contour enhancement. In this step, the original image is dealt with adaptive threshold processing to generate the new contour channel, which merge with the original three-channel image. Then, we employ the pre-trained convolutional neural network with transfer learning to speed up training and contour image channel parameter with Kaiming initialization. Moreover, ArcLoss is applied to increase inter-class differences and intra-class similarity aiming to the high similarity of images of different classes in the dataset.

Results: The accuracy of arteriosclerosis retinopathy grading achieved by the proposed method is up to 65.354%, which is nearly 4% higher than those of the exiting methods. The Kappa of our method is 0.508 in arteriosclerosis retinopathy grading.

Conclusions: An experimental study on multiple metrics demonstrates the superiority of our method, which will be a useful to the toolbox for arteriosclerosis retinopathy grading.
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http://dx.doi.org/10.1016/j.cmpb.2021.106274DOI Listing
July 2021

The association between maternal complications and small for gestational age in twin pregnancies using singleton and twin birth weight references.

J Matern Fetal Neonatal Med 2021 Jul 26:1-7. Epub 2021 Jul 26.

Prenatal Diagnosis Department, The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Introduction: In singleton pregnancies, maternal complications, such as preeclampsia and thyroid dysfunction are associated with small for gestational age (SGA). However, data on the association between SGA and maternal complications in twin pregnancies are limited and conflicting. Small sample size and the application of singleton birth weight reference (SBWR) to define SGA in twins may be reasons for the inconsistent conclusions. Purpose of this study was to define SGA in dichorionic diamniotic (DCDA) and monochorionic diamniotic (MCDA) twin pregnancies using both SBWR and twin birth weight reference (TBWR) and to determine whether certain maternal complications are associated with SGA in twin pregnancies.

Materials And Methods: This retrospective cohort study included all twins delivered in a single tertiary center between 2013 and 2018. SGA was defined as a twin with birth weight <10th centile for gestational age using either SBWR or TBWR. The association between SGA and maternal complications was analyzed separately for DCDA and MCDA twin pregnancies, expressed as odds ratio (OR) and adjusted odds ratio (aOR) with 95% confidence interval (95%-CI).

Results: A total of 2005 DCDA and 467 MCDA twin pregnancies were enrolled. In DCDA pregnancies, SGA was significantly associated with PE according to TBWR (22.73 vs. 14.56%, aOR 1.823, 95%-CI 1.137-2.922). This association was even more pronounced between SGA and severe PE (9.09 vs. 4.54%, aOR 2.234, 95%-CI 1.115-4.479). In turn, PE was associated with higher risk of SGA defined according to TBWR (8.31 vs. 4.99%, aOR 1.825, 95%-CI 1.139-2.925). No association was detected between SGA and other maternal complications. Using SBWR, no association was found between preeclampsia and SGA. In MCDA pregnancies, according to TBWR, SGA was associated only with severe PE (12.5 vs. 4.06%, aOR 3.470, 95%-CI 1.256-9.587) and lower risk of PROM (aOR 0.067, 95%-CI 0.014-0.322).

Conclusion: PE was associated with SGA in DCDA pregnancies only when TBWR was used, suggesting that DCDA pregnancies complicated with PE should be closely monitored for signs of SGA and vice versa. In MCDA pregnancies, SGA defined according to TBWR was associated with only severe PE (but not with all PE) and lower risk of PROM. More basic experiments are needed to investigate the mechanisms underlying PE and SGA in DCDA and MCDA twin pregnancies respectively.
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http://dx.doi.org/10.1080/14767058.2021.1949703DOI Listing
July 2021

Molecular characteristic and pathogenicity analysis of a novel multiple recombinant ALV-K strain.

Vet Microbiol 2021 Jul 21;260:109184. Epub 2021 Jul 21.

Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, PR China. Electronic address:

Avian leukosis virus (ALV) can induce various tumors and cause serious production problems. ALVs isolated from chickens were divided into six subgroups (A-J). In 2012, a strain of a putative novel subgroup of ALVs was isolated from Chinese native chickens in Jiangsu Province and named as ALV-K. In this study, three ALV-K strains (JS14LH01, JS13LH14, and JS15SG01) were isolated from chickens with suspected ALV infection in Jiangsu Province. Their complete genomes were amplified, sequenced, and analyzed systematically. The results showed that JS14LH01 and JS13LH14 were ALV-K and ALV-E recombinant strains. Whereas JS15SG01 is an ALV-K, ALV-E, and ALV-J multiple recombinant strain containing the U3 region of ALV-J. The pathogenicity test of JS15SG01 revealed that, compared with previous ALV-K strains, the viremia and viral shedding level of JS15SG01-infected chickens were significantly increased, reaching 100 % and 59 %, respectively. More important, JS15SG01 induced significant proliferation of gliocytes in the cerebral cortex of infected chickens, accompanied by the neurotropic phenomenon. This is the first report about a multiple recombinant ALV-K strain that could invade and injure the brain tissue of chickens in China. Our findings enriched the epidemiologic data of ALV and helped to reveal the evolution of ALV strains prevalent in chicken fields.
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http://dx.doi.org/10.1016/j.vetmic.2021.109184DOI Listing
July 2021

Raman Thermal Maturity of Coal and Type II Kerogen Based on Surface-Enhanced Raman Spectroscopy (SERS).

ACS Omega 2021 Jul 9;6(28):18504-18508. Epub 2021 Jul 9.

Power, Environmental and Energy Research Institute, Covina, California 91722, United States.

Low-maturity organic samples generate high levels of fluorescence during Raman detection. This fluorescence will obviously affect the Raman signals from organic matter. Our research shows that surface-enhanced Raman spectroscopy (SERS) can significantly enhance the ratio of the Raman signal from organic matter to the fluorescence background without changing the peak positions. This allows us to obtain more accurate Raman parameters for organic matter. In this study, we conducted Raman testing with SERS on coal and type II kerogen from the USA. We found that for both coal and type II kerogen, the exponential correlation between the thermal maturity and the distance between their D and G bands (Δ) in the Raman spectra was good, and the -squared values were 0.968 and 0.988, respectively. However, the Raman thermal maturity evolution curves for the coal and the type II kerogen were different. Compared with the Raman thermal maturity evolution curve of type II kerogen, that of coal was steeper. The two curves crossed each other at a Δ value of 223, which corresponds to a calculated vitrinite reflectance value of 0.61%. This study also shows that the Raman thermal maturity evolution model of organic matter is perhaps related mainly to its type.
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http://dx.doi.org/10.1021/acsomega.1c02730DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296574PMC
July 2021

Ni-Catalyzed Asymmetric Hydrophosphination of Unactivated Alkynes.

J Am Chem Soc 2021 Jul 20. Epub 2021 Jul 20.

Department of Chemistry, University of Science and Technology of China, Hefei 230026, People's Republic of China.

The practical synthesis of -stereogenic tertiary phosphines, which have wide applications in asymmetric catalysis, materials, and pharmaceutical chemistry, represents a significant challenge. A regio- and enantioselective hydrophosphination using cheap and ubiquitous alkynes catalyzed by a nickel complex was designed, in which the toxic and air-sensitive secondary phosphines were prepared from bench-stable secondary phosphine oxides. This methodology has been demonstrated with unprecedented substrate scope and functional group compatibility to afford electronically and structurally diversified P(III) compounds. The products could be easily converted into various precursors of bidentate ligands and organocatalysts, as well as a variety of transition-metal complexes containing both and metal-stereogenic centers.
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http://dx.doi.org/10.1021/jacs.1c05649DOI Listing
July 2021

Sugar-sweetened Beverage Consumption May Modify Associations between Genetic Variants in the CHREBP Locus and HDL-C and TG Concentrations.

Circ Genom Precis Med 2021 Jul 16. Epub 2021 Jul 16.

Department of Clinical Epidemiology, Leiden University Medical Center, Leiden, the Netherlands.

- Carbohydrate responsive element binding protein (ChREBP) is a transcription factor that responds to sugar consumption. Sugar-sweetened beverage (SSB) consumption and genetic variants in the locus have separately been linked to high-density lipoprotein cholesterol (HDL-C) and triglyceride (TG) concentrations. We hypothesized SSB consumption would modify the association between genetic variants in the locus and dyslipidemia. - Data from 11 cohorts from the Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) consortium (N=63,599) and the UK Biobank (UKB) (N=59,220) were used to quantify associations of SSB consumption, genetic variants, and their interaction on HDL-C and TG concentrations using linear regression models. A total of 1,606 single-nucleotide polymorphisms (SNPs) within or near were considered. SSB consumption was estimated from validated questionnaires and participants were grouped by their estimated intake. - In a meta-analysis, rs71556729 was significantly associated with higher HDL-C concentrations only among the highest SSB consumers [β (95% CI) = 2.12 (1.16, 3.07) mg/dl; <0.0002], but not significantly among the lowest SSB consumers (=0.81; <0.0001). Similar results were observed for two additional variants (rs35709627 and rs71556736). For TG, rs55673514 was positively associated with TG concentrations only among the highest SSB consumers [β (95% CI): 0.06 (0.02, 0.09) per allele count for log(mg/dl), =0.001], but not the lowest SSB consumers (=0.84; =0.0005). - Our results identified genetic variants in the locus that may protect against SSB-associated reductions in HDL-C and other variants that may exacerbate SSB-associated increases in TG concentrations.
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http://dx.doi.org/10.1161/CIRCGEN.120.003288DOI Listing
July 2021

Micrometer scale polystyrene plastics of varying concentrations and particle sizes inhibit growth and upregulate microcystin-related gene expression in Microcystis aeruginosa.

J Hazard Mater 2021 Jul 6;420:126591. Epub 2021 Jul 6.

College of Natural Resources and Environment, Northwest A & F University, Yangling 712100, China. Electronic address:

Microplastics (MPs) are a concerning environmental pollutant due to their adverse effects on aquatic organisms. However, the dose- and size-dependent effects of MPs on toxigenic cyanobacteria have not been extensively studied. Herein, we explored the effects of polystyrene MPs (PS-MPs) of varying particle sizes and concentrations on the growth and physiology of Microcystis aeruginosa. The results showed that exposure to 1 µm PS-MPs at a concentration of 2-10 mg L significantly inhibited the growth of M. aeruginosa in a concentration-dependent manner. After 12 days of exposure, high concentrations of 1 µm PS-MPs (≥ 2 mg L) increased levels of reactive oxygen species. Following exposure to 5 mg L PS-MPs of different particle sizes, algal growth was inhibited and oxidative stress was induced by 0.5 and 1 µm PS-MPs. At the molecular level, transcription of the atpB gene was generally downregulated in all PS-MPs treatments, while ftsH and fabZ were upregulated. Exposure to PS-MPs also altered the transcription levels of microcystin-related genes (mcyA and mcyH), causing more microcystin to be produced by M. aeruginosa. The results will be useful for understanding the toxicity of MPs toward toxigenic cyanobacteria, and evaluating the ecological risks of MPs in aquatic environments.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126591DOI Listing
July 2021

Genetically incorporated crosslinkers reveal NleE attenuates host autophagy dependent on PSMD10.

Elife 2021 Jul 13;10. Epub 2021 Jul 13.

Division of Respiratory and Critical Care Medicine, State Key Laboratory of Biotherapy, West China Hospital of Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, China.

Autophagy acts as a pivotal innate immune response against infection. Some virulence effectors subvert the host autophagic machinery to escape the surveillance of autophagy. The mechanism by which pathogens interact with host autophagy remains mostly unclear. However, traditional strategies often have difficulty identifying host proteins that interact with effectors due to the weak, dynamic, and transient nature of these interactions. Here, we found that Enteropathogenic (EPEC) regulates autophagosome formation in host cells dependent on effector NleE. The 26S Proteasome Regulatory Subunit 10 (PSMD10) was identified as a direct interaction partner of NleE in living cells by employing genetically incorporated crosslinkers. Pairwise chemical crosslinking revealed that NleE interacts with the N-terminus of PSMD10. We demonstrated that PSMD10 homodimerization is necessary for its interaction with ATG7 and promotion of autophagy, but not necessary for PSMD10 interaction with ATG12. Therefore, NleE-mediated PSMD10 in monomeric state attenuates host autophagosome formation. Our study reveals the mechanism through which EPEC attenuates host autophagy activity.
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http://dx.doi.org/10.7554/eLife.69047DOI Listing
July 2021

Upgrade of far infrared three-wave polarimeter-interferometer system on Joint-TEXT.

Rev Sci Instrum 2021 May;92(5):053527

International Joint Research Laboratory of Magnetic Confinement Fusion and Plasma Physics, State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

The far infrared polarimeter-interferometer system (POLARIS) on the Joint-TEXT has been upgraded recently. A new support structure has been applied to the mixer array of the POLARIS for adjusting the position of the mixers precisely. Five detection chords have been added to the system on the low field side by optimizing the utilization efficiency of the laser beams on both sides of the beam splitting grid. The spatial resolution can be improved from 3 to 1.5 cm. A better measurement on plasma electron density and current density has been obtained after the system is upgraded. The initial experimental result will be introduced along with the upgrade details.
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http://dx.doi.org/10.1063/5.0040486DOI Listing
May 2021

Development of fetal growth charts in twins stratified by chorionicity and mode of conception: a retrospective cohort study in China.

Chin Med J (Engl) 2021 Jul 7. Epub 2021 Jul 7.

Department of Prenatal Diagnosis, International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China School of Public Health, Shanghai Jiao Tong University, Shanghai 200025, China MOE-Shanghai Key Laboratory of Children's Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China Department of Clinical Nutrition, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Background: Twin pregnancies continue to increase worldwide; however, the current clinical prenatal evaluation for the intrauterine growth of twins still relies on the growth standards of singletons. We attempted to establish a set of fetal biometric references for Chinese twin pregnancies, stratified by chorionicity and conception mode as spontaneously conceived monochorionic diamniotic (SC-MCDA), spontaneously conceived dichorionic diamniotic (SC-DCDA), and assisted reproductive technology dichorionic diamniotic (ART-DCDA) twins.

Methods: From 2016 to 2019, the ultrasonographic fetal biometric measurements were longitudinally collected in pregnant women, including fetal weight, biparietal diameter, head circumference, abdominal circumference, femur length, and humerus length. The linear mixed models were used to test the difference of growth patterns between groups, and the growth curve of each biometric parameter was modeled by a generalized additive model for location scale and shape.

Results: A total of 929 twin pregnant women and 2019 singleton pregnant women, met the inclusion criteria. Among twin pregnancies, 148 were SC-MCDA, 215 were SC-DCDA, and 566 were ART-DCDA twins. Overall, SC-DCDA twins grew faster than SC-MCDA twins, while slower than ART-DCDA twins (all P < 0.05), and all of the three groups showed significant differences comparing with singletons, especially during the third trimester. Hence, the customized fetal growth charts of each fetal biometric parameter were, respectively, constructed for SC-MCDA, SC-DCDA, and ART-DCDA twins.

Conclusions: The fetal biometric trajectories demonstrated characteristic patterns according to chorionicity and conception mode. To fill the gap, we modeled fetal biometric parameters for Chinese SC-MCDA, SC-DCDA, and ART-DCDA twin pregnancies, hoping to provide a reference for the further establishment of fetal growth reference values for Chinese twin fetuses.
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http://dx.doi.org/10.1097/CM9.0000000000001616DOI Listing
July 2021

Naturally occurring mutated infectious bursal disease virus of genotype A8B1 associated with bursa damage in China.

Virus Res 2021 Sep 2;302:198498. Epub 2021 Jul 2.

Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150069, PR China. Electronic address:

Infectious bursal disease virus (IBDV), the causative agent of infectious bursal disease (IBD), mainly damages the bursa of Fabricius, which is a central immune organ of birds. As an RNA virus, IBDV is prone to mutation owing to a combination of factors including natural selection pressure. In this study, a naturally occurring mutated IBDV associated with bursa damage was identified, IBDV-HeN20-7103 strain, in an infected chicken flock in central China. Its full-length genome was cloned, and sequence analysis showed that the IBDV-HeN20-7103 strain was located along with the attenuated IBDV, which corresponds to genotype A8B1 of the recently proposed classification scheme, on the branch of the phylogenetic tree. The amino acid sequence comparisons further highlighted the specific characteristics of IBDV-HeN20-7103 with mutation H253Q compared to the attenuated strain. Animal experiments showed that IBDV-HeN20-7103 could induce serious bursal lesions without mortality, which revealed a unique cause of disease in this flock. The identification of such a strain reaffirms the complexity of IBDV evolution and prevalence.
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http://dx.doi.org/10.1016/j.virusres.2021.198498DOI Listing
September 2021

High-dose Chemotherapy Combined with Autologous Hematopoietic Stem Cell Transplantation as Frontline Therapy for Intermediate/High-risk Diffuse Large B Cell Lymphoma.

Curr Med Sci 2021 Jun 3;41(3):465-473. Epub 2021 Jul 3.

Medical Center of Hematology, Xinqiao Hospital, Army Medical University, Chongqing, 400037, China.

The role of autologous hematopoietic stem cell transplantation (auto-HSCT) following high-dose chemotherapy has been validated and accepted as a standard treatment for patients with relapsed diffuse large B-cell lymphoma (DLBCL). However, its clinical efficacy as frontline therapy remains to be elucidated. This study aimed to examine the feasibility of frontline auto-HSCT for newly diagnosed intermediate/high-risk DLBCL patients. We retrospectively reviewed the data of 223 patients treated with frontline auto-HSCT or chemotherapy alone (year 2008-2014) from four hospitals. The median follow-up time was 29.4 months. Between the two treatment arms among the intermediate/high-risk DLBCL patients, the 3-year overall survival (OS) and progression-free survival (PFS) rates of patients given frontline auto-HSCT were 87.6% and 81.9%, respectively, and the chemotherapy-alone group showed 3-year OS and PFS rates of 64.9% and 59.59%, respectively. Compared with the chemotherapy-alone group, the frontline auto-HSCT could eliminate the adverse impact of non-germinal center B-cell (GCB) type. In addition, in the frontline auto-HSCT group, patients who achieved complete response (CR) at auto-HSCT had a longer survival time than those who did not achieve CR. Our results suggested that frontline auto-HSCT could improve the prognosis of intermediate/high-risk DLBCL patients.
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http://dx.doi.org/10.1007/s11596-021-2394-2DOI Listing
June 2021

Drivers of phenotypic variation in cartilage: Circadian clock genes.

J Cell Mol Med 2021 Jul 2. Epub 2021 Jul 2.

College of Veterinary Medicine, Heilongjiang Key Laboratory Animals and Comparative Medicine, Northeast Agricultural University, Harbin, China.

Endogenous homeostasis and peripheral tissue metabolism are disrupted by irregular fluctuations in activation, movement, feeding and temperature, which can accelerate negative biological processes and lead to immune reactions, such as rheumatoid arthritis (RA) and osteoarthritis (OA). This review summarizes abnormal phenotypes in articular joint components such as cartilage, bone and the synovium, attributed to the deletion or overexpression of clock genes in cartilage or chondrocytes. Understanding the functional mechanisms of different genes, the differentiation of mouse phenotypes and the prevention of joint ageing and disease will facilitate future research.
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http://dx.doi.org/10.1111/jcmm.16768DOI Listing
July 2021

Effects of population flow on regional carbon emissions: evidence from China.

Environ Sci Pollut Res Int 2021 Jul 1. Epub 2021 Jul 1.

School of Economics and Management, China University of Geosciences, Wuhan, 430074, China.

Population flow can affect regional carbon emissions. Based on the analysis of the dual transmission mechanism of population flow and its effect on carbon emissions, this paper empirically studies the impact of population flow and other related factors on China's carbon emissions through panel econometric regression and heterogeneity analysis with fixed effect model. The results show that, firstly, in the long or short term, China's population flow can reduce the growth of carbon emissions. Secondly, the regional population aging and knowledge structure improvement caused by population flow are helpful to reduce carbon emissions, while the regional urbanization improvement caused by population flow is not significantly correlated with the growth of household miniaturization on carbon emissions. Thirdly, from the perspective of heterogeneous geographical divisions, population flow promotes the increase of carbon emissions in the northwest region of the Hu Huanyong Line (Hu Line), while it is opposite in the southeast region of Hu Line. Fourthly, China's consumption level, per capita GDP, energy intensity, and energy consumption structure have contributed to the growth of carbon emissions, while carbon intensity has a negative effect on carbon emissions. Finally, this paper puts forward relevant suggestions from the perspective of coordinating population policy and energy conservation and emission reduction policy.
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http://dx.doi.org/10.1007/s11356-021-15131-7DOI Listing
July 2021

Upregulation of microRNA miR-141-3p and its prospective targets in endometrial carcinoma: a comprehensive study.

Bioengineered 2021 Dec;12(1):2941-2956

Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, P. R. China.

The clinicopathological value of microRNA-141-3p (miR-141-3p) and its prospective target genes in endometrial carcinoma (EC) remains unclear. The present study determined the expression level of miR-141-3p in EC via quantitative real-time PCR (RT-qPCR). RT-qPCR showed a markedly higher expression level of miR-141-3p in EC tissues than in non-EC endometrium tissues ( < 0.0001). The microarray and miRNA-seq data revealed upregulation of miR-141-3p. Integrated analysis based on 675 cases of EC and 63 controls gave a standardized mean difference of 1.737, confirmed the upregulation of miR-141-3p. The Kaplan-Meier survival curve showed that a higher expression of miR-141-3p positively corelated with a poorer prognosis. Combining the predicted targets and downregulated genes in EC, we obtained 271 target genes for miR-141-3p in EC. Two potential targets, PPP1R12A and PPP1R12B, were downregulated at both the mRNA and protein levels. This study indicates that the overexpression of miR-141-3p may play an important part in the carcinogenesis of EC. The overexpression of miR-141-3p may be a risk factor for the prognosis of patients with EC.
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http://dx.doi.org/10.1080/21655979.2021.1943111DOI Listing
December 2021

Synthesis of a Visible-Light-Responsive Perovskite SmTiO N Bifunctional Photocatalyst via an Evaporation-Assisted Layered-Precursor Strategy.

Adv Mater 2021 Jun 27:e2101883. Epub 2021 Jun 27.

State Key Laboratory of Catalysis, iChEM, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian National Laboratory for Clean Energy, 457 Zhongshan Road, Shaheko District, Dalian, Liaoning, 116023, China.

Development of visible-light-responsive oxynitride photocatalysts has been highly inspired for promising solar-to-chemical conversion, but the number of Ti-based oxynitrides is scarce because of the relatively low thermal stability of Ti ions under ammonia flow. Here, the feasible synthesis of a novel perovskite SmTiO N from the layered NaSmTiO precursor is demonstrated to exhibit wide visible-light response with a bandgap of ≈2.1 eV and to show effective water reduction and oxidation functionalities under visible-light irradiation. The successful preparation mainly results from the synergistic effect of the layered structure of NaSmTiO and the evaporation spillover of Na ions, both of which are favorable for ammonia diffusion to accelerate the substitution of nitrogen to oxygen atoms and to shorten the nitridation time. The thermodynamic and kinetic feasibility of SmTiO N for water splitting are investigated in detail, and its optimal apparent quantum efficiency (AQE) of water oxidation reaches 16.7% at 420 ± 10 nm, higher by far than that of most previous visible-light-responsive photocatalysts. Interestingly, a series of oxynitrides RTiO N (R = La, Pr, Nd) are similarly synthesized by the alkali-metal evaporation-assisted layered-precursor strategy, demonstrating its generality to prepare visible-light-responsive (oxy)nitride photocatalysts containing reducible metals for solar energy conversion.
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http://dx.doi.org/10.1002/adma.202101883DOI Listing
June 2021

A two-way rectification method for identifying differentially expressed genes by maximizing the co-function relationship.

BMC Genomics 2021 Jun 25;22(Suppl 1):471. Epub 2021 Jun 25.

School of Computer Science, Northwestern Polytechnical University, 127 Youyi west road, Xi'an, 710072, China.

Background: The identification of differentially expressed genes (DEGs) is an important task in many biological studies. The currently widely used methods often calculate a score for each gene by estimating the significance level in terms of the differential expression. However, biological experiments often have only three duplications, plus plenty of noises contain in gene expression datasets, which brings a great challenge to statistical analysis methods. Moreover, the abundance of gene expression levels are not evenly distributed. Thus, those low expressed genes are more easily to be detected by fold-change based methods, which may results in high false positives among the DEG list. Since phenotypical changes result from DEGs should be strongly related to several distinct cellular functions, a more robust method should be designed to increase the true positive rate of the functional related DEGs.

Results: In this study, we propose a two-way rectification method for identifying DEGs by maximizing the co-function relationships between genes and their enriched cellular pathways. An iteration strategy is employed to sequentially narrow down the group of identified DEGs and their associated biological functions. Functional analyses reveal that the identified DEGs are well organized in the form of functional modules, and the enriched pathways are very significant with lower p-value and larger gene count.

Conclusions: An integrative rectification method was proposed to identify key DEGs and their related functions simultaneously. The experimental validations demonstrate that the method has high interpretability and feasibility. It performs very well in terms of the identification of remarkable functional related genes.
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http://dx.doi.org/10.1186/s12864-021-07772-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229713PMC
June 2021

Baicalein Delays HO-Induced Astrocytic Senescence through Inhibition of Senescence-Associated Secretory Phenotype (SASP), Suppression of JAK2/STAT1/NF-κB Pathway, and Regulation of Leucine Metabolism.

ACS Chem Neurosci 2021 Jul 21;12(13):2320-2335. Epub 2021 Jun 21.

Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China.

Baicalein is an active ingredient extracted from the dried roots of the Georgi. It has been demonstrated to improve memory impairment in multiple animal models; however, the underlying mechanisms remain ambiguous. The accumulation of senescent astrocytes and senescence-associated secretory phenotype (SASP) secreted by senescent astrocytes has been deemed as potential contributors to neurodegenerative diseases. Therefore, this study explored the protective effects of baicalein against astrocyte senescence and investigated the molecular mechanisms and metabolic mechanisms of baicalein against astrocyte senescence. Our results demonstrated that treatment with baicalein protects T98G cells from HO-induced damage, delays cell senescence, inhibits the secretion of SASP (IL-6, IL-8, TNF-α, CXCL1, and MMP-1), and inhibits SASP-related pathways NF-κB and JAK2/STAT1. H NMR metabolomics analysis and correlation analysis revealed that leucine was significantly correlated with SASP factors. Further study demonstrated that supplement with leucine could restrain SASP secretion, and baicalein could significantly increase leucine level through down-regulation of BCAT1 and up-regulation of SLC7A5 expression. The above results revealed that baicalein exerted protective and antisenescence effects in HO-induced T98G cells possibly through inhibition of SASP, suppression of JAK2/STAT1/NF-κB pathway, and regulation of leucine metabolism. Consistent results were obtained in primary astrocytes of newborn SD rats, which suggests that baicalein significantly increases viabilities, delays senescence, inhibits IL-6 secretion, and increases leucine level in HO-induced primary astrocytes.
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http://dx.doi.org/10.1021/acschemneuro.1c00024DOI Listing
July 2021

The clinical value of blood flow parameters of the umbilical artery and middle cerebral artery for assessing fetal distress.

Am J Transl Res 2021 15;13(5):5280-5286. Epub 2021 May 15.

Department of Ultrasound, Qinghai Provincial People's Hospital Xining, Qinghai, P. R. China.

Objective: To explore the clinical value of blood flow parameters of the umbilical artery (UA) and middle cerebral artery (MCA) for gauging fetal distress.

Methods: This study was conducted among 256 pregnant women who presented for routine prenatal checkups and successfully gave birth in our hospital from January 2018 to February 2020. These pregnant women were examined with the Color Doppler Ultrasound, and the color Doppler flow imaging (CDFI) showed the blood flow of the UA and MCA. In accordance with the diagnostic criteria of fetal distress, they were divided into a fetal distress group (n=67) and a control group (n=189). The receiver operator characteristic (ROC) curve analysis was performed on the diagnostic value of the flow in the UA and MCA. According to their pregnancy outcomes, they were divided into a good pregnancy outcome group (n=209) and an adverse pregnancy outcome group (n=47), and their blood flow parameters of the UA and MCA were compared.

Results: The S/D, PI, and RI values of the UA in the fetal distress group exceeded those in the control group, and the S/D, PI, and RI values of the MCA were lower than those in the control group (P<0.05). The good pregnancy outcome group had lower S/D, PI, and RI values of the UA. The good pregnancy outcome group had higher S/D, PI, and RI values of the MCA (P<0.05). ROC curves revealed that the areas under curve of S/D, PI, and RI of the UA were 0.81, 0.76 and 0.74, respectively; the areas under curve of S/D, PI, and RI of the MCA were 0.82, 0.78 and 0.71, respectively.

Conclusion: The hemodynamic indexes of the UA and MCA can be used as a basis for evaluating fetal distress, which shows important clinical indications for gauging pregnancy outcome.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205796PMC
May 2021

Efficacy of intrauterine Bakri balloon tamponade combined with ascending uterine artery ligation on postpartum hemorrhage.

Am J Transl Res 2021 15;13(5):4995-5002. Epub 2021 May 15.

Department of Obstetrics, Tai'an City Central Hospital Tai'an 271000, Shandong Province, China.

Objective: To observe the efficacy of intrauterine Bakri balloon tamponade (IBBT) combined with ascending uterine artery ligation (AUAL) in the treatment of postpartum hemorrhage (PPH) due to uterine inertia after cesarean section.

Methods: A total of 92 patients with PPH due to uterine inertia after cesarean section were divided into a study group (n=46) and a control group (n=46) in accordance with the random number table. The control group was treated with IBBT alone, while the study group was treated with IBBT combined with AUAL. The clinical efficacies, hemorrhage, surgical duration, hospital stay, hemorrhage rate after removal of tamponade, recurrence rate of PPH, changes in coagulation function and quality of life were compared between the two groups.

Results: The overall response rate (ORR) in the study group was 95.65%, remarkably higher than that of 80.43% in the control group ( < 0.05). The study group had a lesser amount of hemorrhage at 2 h and 24 h after surgery, a longer surgical duration, a shorter hospital stay, and lower hemorrhage rate after removal of tamponade and recurrence rate of PPH than the control group ( < 0.05). After treatment, prothrombin time, activated partial thromboplastin time and fibrinogen in the study group were markedly higher than those in the control group ( < 0.05). Compared with those before treatment, the scores of quality of life in the two groups were elevated at 3 months after treatment ( < 0.05), and the scores of quality of life in the study group were higher than those in the control group at 3 months after treatment ( < 0.05).

Conclusion: IBBT combined with AUAL can effectively alleviate hemorrhage and improve coagulation function and quality of life of patients with PPH due to uterine inertia after cesarean section, exhibiting a definite efficacy and a high safety profile.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205799PMC
May 2021

Raddeanin A induced apoptosis of non-small cell lung cancer cells by promoting ROS-mediated STAT3 inactivation.

Tissue Cell 2021 Jun 9;71:101577. Epub 2021 Jun 9.

Department of Thoracic Surgery, Hainan General Hospital, China. Electronic address:

Purpose: Non-small cell lung cancer (NSCLC) is a high-risk type of lung cancer. Raddeanin A exerts anti-tumor activity by regulating cell proliferation and apoptosis, but its role in NSCLC remains to be elucidated. This study was to investigate the effect of raddeanin A in NSCLC and its mechanism.

Methods: The effect of raddeanin A (2, 4, 8, 10 μmol/L) on the viability, proliferation and apoptosis of A549 and H1299 cells was determined by cell counting kit-8, colony formation and flow cytometry assays, respectively. Next, western blot was performed to examine the protein expressions of cleaved caspase-3, Bax, phosphorylated signal transducer and activator of transcription 3 (p-STAT3) and STAT3. Subsequently, the intracellular reactive oxygen species (ROS) generation and mitochondrial membrane potential of NSCLC cells were detected by 2', 7'-dichlorofluorescein-diacetate (DCFH-DA) and JC-1 assay. Lastly, the effect of N-acetylcysteine (NAC) on the apoptosis, ROS generation, and STAT3 was evaluated by the above-mentioned assays again.

Results: Raddeanin A treatment had no obvious effect on 16HBE cells viability, but it inhibited viability and proliferation of A549 and H1299 cells, promoted the apoptosis, increased the protein expressions of cleaved caspase-3 and Bax, generated intracellular ROS, as well as decreased mitochondrial membrane potential and the expressions of p-STAT3 and STAT3 in A549 and H1299 cells. After cells treated with NAC, the effect of raddeanin A was reversed, as evidenced by the apoptosis and ROS generation were suppressed, and the expression of p-STAT3 was promoted.

Conclusion: Raddeanin A suppressed the proliferation and induced apoptosis of NSCLC cells via promoting the ROS-mediated STAT3 inactivation.
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http://dx.doi.org/10.1016/j.tice.2021.101577DOI Listing
June 2021

Gut Microbiota-Related Effects of Tanhuo Decoction in Acute Ischemic Stroke.

Oxid Med Cell Longev 2021 27;2021:5596924. Epub 2021 May 27.

Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.

Acute ischemic stroke (AIS) is a major cause of acquired adult disability and death. Our previous studies proved the efficacy and effectiveness of Tanhuo decoction (THD) on AIS. However, the therapeutic mechanism remains unclear. We recruited 49 AIS patients and 30 healthy people to explore the effects of THD+basic treatment on the poststroke gut microbiota of AIS patients using 16S rRNA sequencing, in which 23 patients received basic treatment (control group) and 26 patients received THD+basic treatment (THD group). By comparing the data before and after treatments, we found the THD group acquired better outcome than the control group on both clinical outcome indices and the characteristics of gut microbiota. In addition to the mediation on short-chain fatty acid- (SCFA-) producing bacteria in two groups, treatment in the THD group significantly decreased the lipopolysaccharide- (LPS-) producing bacteria to reduce LPS biosynthesis. Besides, the complexity of the cooccurrence of gut microbiota and the competition among LPS-producing bacteria and opportunistic pathogenetic bacteria were enhanced in the THD group. Treatment in the THD group also exhibited the potential in decreasing genes on the biosynthesis of trimethylamine (TMA), the precursor of Trimethylamine N-oxide (TMAO), and increasing genes on the degradation of TMA, especially increasing trimethylamine-corrinoid protein Co-methyltransferase () which catabolizes TMA to methane. These results hinted that THD+basic treatment might exert its efficacy by mediating the gut microbiota and microbial metabolites, including LPS and TMAO that aggravate the sterile inflammation and platelet aggregation. Moreover, the well-fitting regression model results in predicting the clinical outcome with the alteration of gut microbiota proved gut microbiota as a potential indicator of AIS and provided evidence of the communication between the gut and brain of AIS patients.
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http://dx.doi.org/10.1155/2021/5596924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175183PMC
May 2021

Free ammonia pretreatment enhances the removal of antibiotic resistance genes in anaerobic sludge digestion.

Chemosphere 2021 Sep 19;279:130910. Epub 2021 May 19.

Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, NSW, 2007, Australia. Electronic address:

Sludge has been recognized as a reservoir of antibiotic resistance genes (ARGs) in the wastewater treatment plants. Our previous study has demonstrated that free ammonia (FA, i.e., NH-N) pretreatment is an effective method for enhancing anaerobic digestion of sludge. However, the effects of FA pretreatment on the removal of ARGs in the anaerobic sludge digestion is still unknown. In this study, several ARGs representing various antibiotic classes and integrase gene (intI1) which is crucial for horizontal transfer of ARGs were chosen. This study demonstrated that combined FA pretreatment (420 mg NH-N/L for 24 h, under which the highest anaerobic sludge biodegradability was achieved in our previous study) and anaerobic digestion could enhance the removal of aac(6')-Ib-cr, blaTEM, sul2, tetA, tetB and tetX from sludge by 17-74% compared with anaerobic digestion without FA pretreatment, resulting in a lower ARGs abundance in the anaerobically digested sludge. This is caused by the removal of tested ARGs during FA pretreatment and the reduced abundance of potential microbial hosts of ARGs due to FA pretreatment during anaerobic digestion. The removal of IntI1 was not significantly affected by FA pretreatment and intI1 did not play a large role in the fate of the tested ARGs in this study. This study indicated that FA pretreatment for anaerobic digestion could potentially reduce the spread of ARGs from the sludge to the natural environment during sludge disposal or reuse.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130910DOI Listing
September 2021

A Single Amino Acid at Residue 188 of Hexon Protein is Responsible for the Pathogenicity of the Emerging Novel Fowl Adenovirus 4.

J Virol 2021 Jun 16:JVI0060321. Epub 2021 Jun 16.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.

Since 2015, severe hydropericardium-hepatitis syndrome (HHS) associated with a novel (FAdV-4) has emerged in China, representing a new challenge for the poultry industry. Although various highly pathogenic FAdV-4 strains have been isolated, the virulence factor and the pathogenesis of novel FAdV-4 are unclear. In our previous studies, we reported that a large genomic deletion (1966 bp) is not related to increased virulence. In this study, two recombinant chimeric viruses, rHN20 strain and rFB2 strain, were generated from a highly pathogenic FAdV-4 strain by replacing or gene of a non-pathogenic FAdV-4, respectively. Both chimeric strains showed similar titers to the wild type strain . Notably, rFB2 and the wild type strain induced 100% mortality, while no mortality or clinical signs appeared in chickens inoculated with rHN20, indicating that hexon, but not fiber-2, determines the novel FAdV-4 virulence. Furthermore, an R188I mutation in the hexon protein identified residue 188 as the key amino acid for the reduced pathogenicity. The rR188I mutant strain was significantly neutralized by chicken serum and , whereas the wild type strain was able to replicate efficiently. Finally, the immunogenicity of the rescued rR188I was investigated. Non-pathogenic rR188I provided full protection against lethal FAdV-4 challenge. Collectively, these findings provide an in-depth understanding of the molecular basis of novel FAdV-4 pathogenicity and present rR188I as a potential live attenuated vaccine candidate or a novel vaccine vector for HHS vaccines. HHS associated with a novel FAdV-4 infection in chickens has caused huge economic losses to the poultry industry in China since 2015. The molecular basis for the increased virulence remains largely unknown. Here, we demonstrate that the hexon gene is vital for FAdV-4 pathogenicity. Furthermore, we show that the amino acid residue at position 188 of the hexon protein is responsible for pathogenicity. Importantly, the rR188I mutant strain was neutralized by chicken serum and , whereas the wild type strain was not. Further, the rR188I mutant strain provided complete protection against FAdV-4 challenge. Our results provide a molecular basis of the increased virulence of novel FAdV-4. We propose that the rR188I mutant is a potential live attenuated vaccine against HHS and a new vaccine vector for HHS-combined vaccines.
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http://dx.doi.org/10.1128/JVI.00603-21DOI Listing
June 2021

Age-Associated Proteomic Signatures and Potential Clinically Actionable Targets of Colorectal Cancer.

Mol Cell Proteomics 2021 Jun 12;20:100115. Epub 2021 Jun 12.

Department of Gastrointestinal Surgery, National Clinical Research Center for Geriatrics and Department of General Practice, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu, China. Electronic address:

The occurrence and prevalence of colorectal cancer (CRC) is closely associated with age. More than 90% of patients with CRC are diagnosed after 50 years of age. However, CRC incidence of young individuals has been increasing since 1990s, whereas the overall CRC frequency is declining. Distinct overall survival rates between young and aged patients with CRC have been established. Tremendous efforts have been made to clarify the underlying mechanisms of age-dependent clinical differences, but it still remains elusive. Here, we performed proteomic profiling of 50 patients with CRC and revealed proteomic signatures of CRC across age groups. Gene set enrichment analysis showed that distinct age-dependent clinical outcomes might mainly attribute to varied MYC targets V1/V2, E2F targets and G2M checkpoint gene sets, which were associated with cancer cell proliferation, cell apoptosis, tumor growth, and tumor metastasis. Multiple linear regression analysis revealed a large number of functional proteins, such as NOP2, CSE1L, NHP2, NOC2L and CDK1, with adjusted expression significantly correlated with age (p < 0.05). Among them, NHP2 is a core component of the telomerase complex associated with age. High NHP2 expression predicted poor overall survival, with a more significant correlation in aged patients with CRC. Knockdown of NHP2 significantly suppressed cancer cell proliferation. In addition, we revealed some age-related potential clinically actionable targets, such as PSEN1, TSPO, and CDK1, which might be more suitable for patients with late-onset CRC. Collectively, this study identifies age-associated proteomic signatures and potential therapeutic targets of CRC and may help make a precise decision on CRC treatment.
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http://dx.doi.org/10.1016/j.mcpro.2021.100115DOI Listing
June 2021

Nanoscale, automated, high throughput synthesis and screening for the accelerated discovery of protein modifiers.

RSC Med Chem 2021 May 5;12(5):809-818. Epub 2021 May 5.

Pharmacy Department, Drug Design group, University of Groningen A. Deusinglaan 1 9700 AD Groningen The Netherlands

Hit finding in early drug discovery is often based on high throughput screening (HTS) of existing and historical compound libraries, which can limit chemical diversity, is time-consuming, very costly, and environmentally not sustainable. On-the-fly compound synthesis and screening in a highly miniaturized and automated format has the potential to greatly reduce the medicinal chemistry environmental footprint. Here, we used acoustic dispensing technology to synthesize a library in a 1536 well format based on the Groebcke-Blackburn-Bienaymé reaction (GBB-3CR) on a nanomole scale. The unpurified library was screened by differential scanning fluorimetry (DSF) and cross-validated using microscale thermophoresis (MST) against the oncogenic protein-protein interaction menin-MLL. Several GBB reaction products were found as μM menin binder, and the structural basis of the interactions with menin was elucidated by co-crystal structure analysis. Miniaturization and automation of the organic synthesis and screening process can lead to an acceleration in the early drug discovery process, which is an alternative to classical HTS and a step towards the paradigm of continuous manufacturing.
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http://dx.doi.org/10.1039/d1md00087jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152715PMC
May 2021

Variation in the content and fluorescent composition of dissolved organic matter in soil water during rainfall-induced wetting and extract of dried soil.

Sci Total Environ 2021 Jun 9;791:148296. Epub 2021 Jun 9.

College of Natural Resources and Environment, Northwest A & F University, Yangling 712100, PR China. Electronic address:

Dissolved organic matter (DOM) is present in all soils, providing a readily available carbon source for microorganisms, which influences microbially mediated biogeochemical processes. Rainfall-induced wetting can alter the content and composition of soil DOM. However, conventional methods commonly used to extract DOM from soils involve air- or oven-drying followed by extraction with water, and the results vary considerably in terms of indexes used. Therefore, this study aimed to determine the variation in DOM content and composition of soil gravity water and capillary water during wetting, and establish a better method to obtain real soil DOM information. Following simulated rainfall (50 mm h, 0-24 h), gravity water and capillary water in fresh soil samples were separated using a high-speed refrigerated centrifuge. Additionally, DOM in dried soil samples was extracted using various soil/water ratios after drying by different methods. The DOM data obtained by conventional methods were compared with capillary water data. The results showed that DOM degradation occurred mainly in capillary water close to the surface of soil particles. Among the six fluorescent components of DOM identified, a tryptophan-like component (E/E = 295/335 nm and 230/335 nm) was possibly derived from terrestrial plants, and a tyrosine-like component (E/E = 265/305 nm) was likely derived from microbial secretion. Except for little variation in the fluorescence index, dissolved organic carbon concentrations in capillary water were double those in dried soil extracted by conventional methods. The humification index and spectral slope ratio of DOM extracted by conventional methods also markedly varied, and no clear patterns were observed for the variation in specific UV absorbance at 254 nm. These findings allow real information to be obtained regarding soil DOM during wetting, and better selection of the extraction method and indexes when studying soil DOM.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148296DOI Listing
June 2021

[The Factors Related to Treatment Failure in Children with Acute Lymphoblastic Leukemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Jun;29(3):661-668

Department of Hematology, Children's Hospital of Soochow University, Suzhou 215000, Jiangsu Province, China,E-mail:

Objective: To analyze the efficacy of CCLG-ALL-2008 protocol and the related factors of treatment failure in children with acute lymphoblastic leukemia (ALL).

Methods: The clinical data of 400 children newly-diagnosed ALL in Children's Hospital of Soochow University from March 1, 2008 to December 31, 2012 was retrospectively analyzed. All the children accepted CCLG-ALL-2008 protocol, and were followed-up until October 2019. The dates of relapse, death and causes of death were recorded. Treatment failure was defined as relapse, non-relapse death, and secondary tumor.

Results: Following-up for 10 years, there were 152 cases relapse or non-relapse death, the treatment failure rate was 38%, including 122 relapse (80.3%), 30 non-relapse deaths (19.7%) which included 7 cases (4 cases died of infection and 3 cases died of bleeding) died of treatment (23.3% of non-relapse deaths), 8 cases died of minimal residual disease (MRD) continuous positive (26.7% of non-relapse deaths) and 15 cases died of financial burden (50% of non-relapse deaths). According to the relapse stage, 37 cases (30%) in very early stage, 38 cases (31%) in early stage, and 47 cases (39%) in late stage, while according to the relapse site, 107 cases relapsed in bone marrow, 3 cases in testis, 3 cases in central nervous system (CNS), 5 cases in bone marrow plus testis and 4 cases in bone marrow plus CNS. Bone marrow relapse was the main cause of death in 89 cases, followed by nervous system. Initially diagnosed WBC count (≥50×10/L), T-cell immunophenotype, and MRD-positive at week 12 were the independent risk prognostic factors for relapse in children with ALL, while age (≥10 years), initially diagnosed WBC count (≥50×10/L), M3 bone marrow on day 15, and MRD-positive at week 12 were the independent risk factors due to treatment failure. No secondary tumors were found during the follow-up for 10 years.

Conclusion: Relapse is the main cause of treatment failure in children with ALL. The initially diagnosed WBC count, immunophenotype and MRD at week 12 were the independent prognostic factors for relapse of the patients. Financial burden accounts for a large proportion of non-relapse death.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.03.002DOI Listing
June 2021

A human antibody of potent efficacy against SARS-CoV-2 in rhesus macaques showed strong blocking activity to B.1.351.

MAbs 2021 Jan-Dec;13(1):1930636

Shanghai Jemincare Pharmaceuticals Co., Ltd., Shanghai, People's Republic of China.

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), which causes coronavirus disease-2019 (COVID-19), interacts with the host cell receptor angiotensin-converting enzyme 2 (hACE2) via its spike 1 protein during infection. After the virus sequence was published, we identified two potent antibodies against the SARS-CoV-2 receptor binding domain (RBD) from antibody libraries using a phage-to-yeast (PtY) display platform in only 10 days. Our lead antibody JMB2002, now in a Phase 1 clinical trial (ChiCTR2100042150), showed broad-spectrum blocking activity against hACE2 binding to the RBD of multiple SARS-CoV-2 variants, including B.1.351 that was reportedly much more resistant to neutralization by convalescent plasma, vaccine sera and some clinical-stage neutralizing antibodies. Furthermore, JMB2002 has demonstrated complete prophylactic and potent therapeutic efficacy in a rhesus macaque disease model. Prophylactic and therapeutic countermeasure intervention of SARS-CoV-2 using JMB2002 would likely slow down the transmission of currently emerged SARS-CoV-2 variants and result in more efficient control of the COVID-19 pandemic.
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http://dx.doi.org/10.1080/19420862.2021.1930636DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189090PMC
June 2021

Incidence and risk factors of congenital heart disease in Qingdao: a prospective cohort study.

BMC Public Health 2021 06 2;21(1):1044. Epub 2021 Jun 2.

Qingdao Women and Children's Hospital, Qingdao University, Qingdao, Shandong Province, No.6 Tongfu Road, Qingdao, 266000, China.

Background: Many studies have been conducted to assess the incidence of congenital heart disease (CHD). However, results were greatly inconsistent among these studies with a broad range of findings.

Methods: A prospective census-based cohort study was conducted in Qingdao, China, from August 1, 2018 to April 30, 2019. All of the local registered pregnant women were continuously investigated and followed from 15 to 20 weeks of gestation to delivery, tracking the CHD cases in both the fetal and neonatal stages. A logistic regression model was applied to assess the association between CHD and possible risk factors.

Results: The positive rate of prenatal CHD screening was 14.36 per 1000 fetuses and the incidence of CHD was 9.38 per 1000 live births. Results from logistic regression indicated that, living in the countryside (odds ratio, (OR): 0.771; 95% confidence interval, (CI): 0.628-0.946) and having a childbearing history (OR: 0.802; 95%CI: 0.676-0.951) were negatively associated with CHD. However, twin pregnancy (OR: 1.957, 95% CI: 1.245-3.076), illness in the first trimester (OR: 1.306; 95% CI: 1.048-1.628), a family history of CHD (OR: 7.156; 95% CI: 3.293-15.552), and having a child with a birth defect (OR: 2.086; 95% CI: 1.167-3.731) were positively associated with CHD.

Conclusion: CHD is a serious health problem in Qingdao. The CHD incidence found in this study was similar to existing research. The positive rate of prenatal CHD screening was higher than the incidence of neonatal CHD. Moreover, CHD risk factors were identified in our study, and our findings may have great implications for formation CHD intervention strategies.
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http://dx.doi.org/10.1186/s12889-021-11034-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173734PMC
June 2021
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