Publications by authors named "Li Feng"

5,422 Publications

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Differential expression profiles of microRNAs in musk gland of unmated and mated forest musk deer ().

PeerJ 2021 22;9:e12710. Epub 2021 Dec 22.

Sichuan Agricultural University, Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Chengdu, Sichuan, China.

Background: The formation of musk is a complex biophysical and biochemical process that change with the rut of male forest musk deer. We have reported that the mating status of male forest musk deer might result to the variations of chemical composition and microbiota of musk and its yields. Critical roles for microRNAs (miRNAs) of multi-tissues were profiled in our previous study; however, the role for miRNAs of the musk gland remains unclear in this species.

Methods: In this study, we used Illumina deep sequencing technology to sequence the small RNA transcriptome of unmated male (UM) and mated male (UM) of Chinese forest musk deer.

Results: We identified 1,652 known miRNAs and 45 novel miRNAs, of which there were 174 differentially expressed miRNAs between UM and MM. chi-miR-21-5p, ipu-miR-99b and bta-miR-26a were up-regulated in UM among the 10 most differentially expressed miRNAs. Functional enrichment of the target genes showed that monosaccharide biosynthetic process, protein targeting, cellular protein catabolic process enriched higher in MM. Meanwhile, structural molecule activity, secretion by cell, regulated exocytosis and circulatory system process enriched more in UM, hinting that the formation of musk in UM was mediated by target genes related to exocytosis. The miRNA-mRNA pairs such as miR-21: , miR143: , miR-141/200a: might involve in musk gland development and musk secretion, which need to be verified in future study.
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http://dx.doi.org/10.7717/peerj.12710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8710055PMC
December 2021

Progressive osseous heteroplasia: a case report and literature review.

Am J Transl Res 2021 15;13(12):14109-14114. Epub 2021 Dec 15.

Department of Orthopedics, The First Affiliated Hospital of Hunan Medical College Huaihua 418000, Hunan, China.

Objective: To investigate the clinical features and pathogenesis of progressive osseous heteroplasia (POH) in children.

Methods: The clinical features and imaging findings of a child with POH are described, and family investigations and gene comparisons were performed, followed by a literature review.

Results: A 9-year-old female with no relevant family medical history initially presented with ectopic ossification of the skin and subcutaneous tissue of the right face that developed slowly. The ossification area extended to the right waist, back, and right knee. The unilateral body (limbs) was gradually invaded. The patient exhibited limited movement of the head, neck, and left shoulder joint, and experienced difficulty in opening her mouth. She also exhibited deformity of the toe, delayed development, insufficient language skills and behavioral ability, and difficulty in communicating with others, but had no apparent endocrine disorders. Blood calcium, phosphorus, and alkaline phosphatase levels were normal, and DNA sequencing did not yield a positive result.

Conclusion: The clinical manifestations of POH include hard plaques, which can develop deep into the bone; however, there are currently no effective preventive or treatment measures.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748116PMC
December 2021

Altered effective connectivity in migraine patients during emotional stimuli: a multi-frequency magnetoencephalography study.

J Headache Pain 2022 Jan 15;23(1). Epub 2022 Jan 15.

Department of Neurology, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, 210029, Jiangsu, China.

Background: Migraine is a common and disabling primary headache, which is associated with a wide range of psychiatric comorbidities. However, the mechanisms of emotion processing in migraine are not fully understood yet. The present study aimed to investigate the neural network during neutral, positive, and negative emotional stimuli in the migraine patients.

Methods: A total of 24 migraine patients and 24 age- and sex-matching healthy controls were enrolled in this study. Neuromagnetic brain activity was recorded using a whole-head magnetoencephalography (MEG) system upon exposure to human facial expression stimuli. MEG data were analyzed in multi-frequency ranges from 1 to 100 Hz.

Results: The migraine patients exhibited a significant enhancement in the effective connectivity from the prefrontal lobe to the temporal cortex during the negative emotional stimuli in the gamma frequency (30-90 Hz). Graph theory analysis revealed that the migraine patients had an increased degree and clustering coefficient of connectivity in the delta frequency range (1-4 Hz) upon exposure to positive emotional stimuli and an increased degree of connectivity in the delta frequency range (1-4 Hz) upon exposure to negative emotional stimuli. Clinical correlation analysis showed that the history, attack frequency, duration, and neuropsychological scales of the migraine patients had a negative correlation with the network parameters in certain frequency ranges.

Conclusions: The results suggested that the individuals with migraine showed deviant effective connectivity in viewing the human facial expressions in multi-frequencies. The prefrontal-temporal pathway might be related to the altered negative emotional modulation in migraine. These findings suggested that migraine might be characterized by more universal altered cerebral processing of negative stimuli. Since the significant result in this study was frequency-specific, more independent replicative studies are needed to confirm these results, and to elucidate the neurocircuitry underlying the association between migraine and emotional conditions.
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http://dx.doi.org/10.1186/s10194-021-01379-4DOI Listing
January 2022

PD-L1 expression and Tumor mutation burden as Pathological response biomarkers of Neoadjuvant immunotherapy for Early-stage Non-small cell lung cancer: A systematic review and meta-analysis.

Crit Rev Oncol Hematol 2022 Jan 11:103582. Epub 2022 Jan 11.

Department of Thoracic Surgery and Oncology, The First Affiliated Hospital of Guangzhou Medical University, State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, Guangzhou, China. Electronic address:

To date, there is no approved biomarker for predicting pathological response in neoadjuvant programmed cell death (ligand) 1 (PD-(L)1) blockades treated early-stage non-small cell lung cancer (NSCLC). Databases including PubMed, Embase, ClinicalTrials.gov, and Conference abstracts were searched for clinical trials of neoadjuvant PD-1/PD-L1 blockades for resectable NSCLC. Data regarding major pathological response (MPR), pathological complete response (pCR) in patients with high/low pretreatment PD-L1 expression, and tumor mutation burden (TMB) were synthesized using fixed-model meta-analysis and evaluated by odds ratio with 95% confidence interval. This analysis included 10 studies involving 461 NSCLC patients. Compared with PD-L1 expression <1%, PD-L1 expression ≥1% is associated with a higher rate of MPR and pCR. High-TMB associated with MPR and pCR. Similar findings were observed in subgroup analyses despite mono-PD-1/PD-L1 blockade or their combination with chemotherapy. Notably, 50% as the cutoff value for PD-L1 expression demonstrated better prediction efficacy for MPR than that of 1%.
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http://dx.doi.org/10.1016/j.critrevonc.2022.103582DOI Listing
January 2022

Event-triggered H/passive synchronization for Markov jumping reaction-diffusion neural networks under deception attacks.

ISA Trans 2021 Dec 30. Epub 2021 Dec 30.

School of Electrical and Information Engineering, Anhui University of Technology, Ma' anshan 243032, China.

The issue of H/passive master-slave synchronization for Markov jumping neural networks with reaction-diffusion terms is investigated in this paper via an event-triggered control scheme under deception attacks. To lighten the burden of limited communication bandwidth as well as ensure the control performance, an event-triggered transmission scheme is developed. Meanwhile, the randomly occurring deception attacks, which received from the event generator are assumed to modify the sign of the control signal, are taken into account. Furthermore, sufficient conditions ensuring the prescribed H/passive performance level of the neural networks, are deduced beyond Lyapunov stability theory, and the controller gains are derived dealing with the matrix convex optimization problem. At last, the availability of the approach proposed is demonstrated via a numerical example.
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http://dx.doi.org/10.1016/j.isatra.2021.12.035DOI Listing
December 2021

Coherent combining of a large-scale fiber laser array over 2.1 km in turbulence based on a beam conformal projection system.

Opt Lett 2022 Jan;47(2):365-368

We demonstrated a beam conformal projection system for coherent combining of large-scale lasers over 2.1 km in turbulence 20 m above the ground, using the basic modules of a 19-element fiber phased array combined with coarse pointing by a gimbal mount. After coarse pointing and aberration corrections, the metrics (reflected light) of the combined beams from a basic module were best increased by 13.4 times, suggesting that our system promises the great effect of coherent combining under long-distance turbulence. Moreover, we tentatively realized coherent combining of two basic modules (38 lasers), which is the largest number of elements in a fiber laser coherent beam combination outdoors, to the best of our knowledge, with the metrics of combined beams increased by about 29 times.
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http://dx.doi.org/10.1364/OL.446722DOI Listing
January 2022

Validity and reliability of the Chinese version of the epilepsy stigma scale.

Epilepsy Behav 2022 Jan 11;127:108531. Epub 2022 Jan 11.

Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, PR China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China. Electronic address:

Objective: This study was conducted to test the validity and reliability of the Chinese version of the epilepsy stigma scale (ESS), which aims to better understand the stigma of patients with epilepsy (PWEs), lays the foundation for future investigation and explores appropriate strategies to mitigate PWEs' stigma in Chinese culture.

Methods: The scale was translated following standard procedures. For psychometric validation, the Chinese version of the ESS was administered to 214 PWEs above the age of 16 who were diagnosed with epilepsy by two trained epileptologists and were taking anti-seizure drugs for at least a month. All of the patients were recruited from Xiangya Hospital of Central South University of China from August 2021 to September 2021.

Results: The Cronbach's alpha coefficient was 0.893 for the entire scale, 0.903 for felt stigma, and 0.688 for enacted stigma. Exploratory and confirmatory factor analyses were conducted and showed that the scale was grouped under two dimensions, and the results of confirmatory factor analysis support the structure.

Conclusion: The Chinese version of the ESS is a valid and reliable tool to assess epilepsy-related stigma in Chinese culture.
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http://dx.doi.org/10.1016/j.yebeh.2021.108531DOI Listing
January 2022

Association of blood cobalt concentrations with dyslipidemia, hypertension, and diabetes in a US population: A cross-sectional study.

Medicine (Baltimore) 2022 Jan;101(2):e28568

Department of Breast and Thyroid Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.

Abstract: Various heavy metal elements in the human body have been reported to be associated with dyslipidemia, hypertension, and diabetes. The role of cobalt in these conditions is unclear. The current study aimed to investigate the association of blood cobalt concentrations with dyslipidemia, hypertension, and diabetes.Using the data collected from the National Health and Nutrition Examination Survey (2015-2018), we performed logistic regression to explore the association of blood cobalt concentrations with total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglycerides, hypertension, and diabetes.A total of 6866 adults were included in this study. Participants with higher blood cobalt levels appeared to be older and have a lower body mass index and, were more likely to be female (P for trend < .05). After fully adjusting for demographic characteristics (Model 2), compared with the lowest quartile, the highest quartile of blood cobalt concentrations had lower odds ratios (ORs) for elevated TC [OR: 0.62, 95% confidential interval (CI): 0.53 to 0.72, P < .001], elevated LDL-C (OR: 0.65, 95% CI: 0.53-0.80, P < .001) and low HDL-C (OR: 0.81, 95% CI: 0.69-0.96, P = .013). The adjusted ORs for elevated TC, elevated LDL-C and low HDL-C were negatively correlated with increased blood cobalt concentrations (P for trend < .05). The adjusted ORs for hypertension and diabetes were not associated with blood cobalt concentrations (P > .05 and P for trend > .05).In conclusion, higher blood cobalt concentrations were associated with a lower risk of dyslipidemia. However, blood cobalt concentrations were not associated with the risk of hypertension or diabetes.
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http://dx.doi.org/10.1097/MD.0000000000028568DOI Listing
January 2022

Photo-Assisted Robust Anti-Interference Self-Powered Biosensing of MicroRNA Based on Pt-S Bonds and the Inorganic-Organic Hybridization Strategy.

Anal Chem 2022 Jan 13. Epub 2022 Jan 13.

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, People's Republic of China.

Photo-assisted biofuel cell-based self-powered biosensors (PBFC-SPBs) possess the advantages of no need for external power supply, ease of sensing design, and simple instruments. In this work, a robust anti-interference PBFC-SPB for microRNA detection was constructed based on the Pt-S bond and the inorganic-organic hybridization strategy. The organic semiconductor [6,6]-phenyl-C61-butyric acid [email protected] ([email protected]) served as an efficient light-harvesting material, and gold [email protected] ([email protected]) nanomaterials were immobilized on the surface via electrostatic adsorption for the binding of DNA. Notably, compared to Au-S bonds for DNA immobilization, the Pt-S bond exhibited better anti-interference ability. Ingeniously, cadmium sulfide quantum dots (CdS QDs) were close to the [email protected] substrate electrode to form sensitization structures, which was beneficial to enhance the photocurrent signal. Combining with the laccase-mimicking activity Cu/carbon nanotubes (Cu/CNTs) cathode, the PBFC-SPB for microRNA detection was achieved. Once the target existed, the identical sequence complementary microRNA would make DNA2/CdS dissociate and break away from the electrode, leading to a low signal. The linear detection range was 10 fM-100 pM, with the limit of determination of 2.4 fM (3/). The as-proposed strategy not only paves a new way for the design of photoelectrochemical biosensing but also opens a door for the construction of robust anti-interference bioassay for microRNA detection.
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http://dx.doi.org/10.1021/acs.analchem.1c04135DOI Listing
January 2022

Migration characteristics as a prognostic factor in cerebral sparganosis.

Int J Infect Dis 2022 Jan 8. Epub 2022 Jan 8.

Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, 410008, P.R. China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China. Electronic address:

Objective: To differentiate diagnostic and prognostic factors from the clinical material of patients with cerebral sparganosis in central South China.

Methods: Consecutive patients with cerebral sparganosis from our hospital between 2010 and 2018 were retrospectively enrolled. The clinical manifestations, radiographic features, treatment and outcomes of these patients were analysed.

Results: Thirty patients with cerebral sparganosis were included, and foci migration on magnetic resonance imaging was detected in 22 patients, from whom we observed four migration modes: 1) interlobar migration (50.0%, 11/22); 2) trans-midline migration (27.3%, 6/22); 3) trans-ventricular migration (13.6%, 3/22); and 4) cerebellum-brainstem migration (9.1%, 2/22). The percentage of good outcomes was higher in patients with live worm capture than in those without live worm capture (75.0%, 12/16 vs 33.3%, 2/6). Exposure to preoperative anti-parasitic medication was associated with worm migration towards the cortical surface, which led to a higher probability of live worm capture.

Conclusions: We propose four modes of sparganosis migration that are correlated with worm capture and neurological prognosis. We found that exposure to anti-parasitic medication was associated with worm migration towards the cortical surface, leading to a higher probability of live worm capture. These observations suggest a novel significance for preoperative medication of cerebral sparganosis.
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http://dx.doi.org/10.1016/j.ijid.2022.01.005DOI Listing
January 2022

Transcriptome analysis revealing molecular mechanisms of enhanced pigment yield by succinic acid and fluconazole.

Prep Biochem Biotechnol 2022 Jan 11:1-11. Epub 2022 Jan 11.

Department of Bioengineering, College of Life Science, Huaibei Normal University, Huaibei, China.

This study aimed to elucidate the molecular mechanisms through which succinic acid and fluconazole stimulate pigment biosynthesis under liquid fermentation conditions. The pigment yield was significantly improved by adding 0.35 g·L succinic acid or 1.5 g·L fluconazole. Transcriptome sequencing and RT-qPCR confirmation were performed to reveal transcriptome changes. The results indicated that the addition of succinic acid significantly decreased mRNA expression of genes involved in fatty acid biosynthesis while increasing expression of genes involved in pyruvate metabolism. Fluconazole significantly down-regulated transcripts involved in branched-chain amino acid metabolism, fatty acid metabolism, glycolysis/gluconeogenesis, and pyruvate metabolism, as well as the generation of acetyl-CoA for pigment biosynthesis. On the other hand, nitrogen metabolism and lysine degradation pathways were significantly enriched, which could stimulate the generation of acetyl-CoA. Therefore, the mechanism for enhancing pigment yield may be attributed to the competitive regulation of metabolic pathways toward acetyl-CoA biosynthesis. Additionally, up-regulation of some different key genes in the presence of fluconazole or succinic acid was involved in improving pigment production. This study deepens the theoretical understanding for enhancing pigment biosynthesis and provides a few potential approaches for improving pigment yield.
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http://dx.doi.org/10.1080/10826068.2021.2023822DOI Listing
January 2022

X-linked myopathy with excessive autophagy due to a mutation in VMA21 gene: the first case in China.

Neurol Sci 2022 Jan 11. Epub 2022 Jan 11.

Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University, 58 2nd Zhongshan Road, Guangzhou, 510080, People's Republic of China.

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http://dx.doi.org/10.1007/s10072-021-05788-wDOI Listing
January 2022

Maternal embryonic leucine zipper kinase serves as a potential prognostic marker and leads to sorafenib chemoresistance modified by miR-142-5p in hepatocellular carcinoma.

Mol Biol Rep 2022 Jan 10. Epub 2022 Jan 10.

Department of Clinical Laboratory, Affiliated Hospital of Nantong University, Xisi Road, Nantong, 226001, Jiangsu, China.

Background: Chemotherapy is an important treatment strategy for advanced hepatocellular carcinoma (HCC). Sorafenib is a first-line systemic drug that has been commonly used clinically for patients with advanced HCC. However, the high resistance rate of sorafenib in HCC patients often hinders its long-term efficacy. Therefore, it is vital to reveal the molecular mechanisms of sorafenib resistance in patients with HCC.

Methods: In current study, we screened out fourteen genes that over-expressed in HCC specimens through integrative bioinformatics analysis. Here, maternal embryonic leucine zipper kinase (MELK) was highlighted as one of the most probable molecules. The Database for Annotation Visualization and Integrated Discovery (DAVID) program was utilized for functional pathway enrichment analysis. Real-time PCR (RT-PCR) and western blot were used to examine the expression levels of MELK. CCK-8, transwell, colony formation assays and flow cytometry were used to detect cell proliferation, the cell cycle. The dual luciferase assays were performed to study the targeting relationship between MELK and miR-142-5p.

Results: MELK expressions were correlated significantly with cell proliferation by regulating cell cycle and DNA replication. High MELK expression in patients with HCC indicated a poor prognosis both the overall and diseases free survival rates. MELK knockdown suppresses cell proliferation, migration and invasion in vitro. miR-142-5p regulates MELK expression through binding to the complementary sequence in the 3'-UTR regions. MELK knockdown enhances sensitivity of sorafenib in HCC sorafenib-resistant (HCC/SR) cells.

Conclusions: MELK may serve as a potential prognostic marker in HCC and MELK knockdown enhanced sensitivity of HepG2/SR cells to sorafenib treatment. Our findings suggest that MELK/miR-142-5p axis could be a potentially therapeutic target for reversing the sorafenib resistance in HCC treatment.
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http://dx.doi.org/10.1007/s11033-022-07128-3DOI Listing
January 2022

Simple construction of a two-component fluorescent sensor for turn-on detection of Hg in human serum.

Anal Bioanal Chem 2022 Jan 11. Epub 2022 Jan 11.

National Engineering Research Center of Coal Preparation & Purification, Key Laboratory of Coal Processing & Efficient Utilization of Ministry of Education, School of Chemical Engineering & Technology, China University of Mining & Technology, Xuzhou, 221116, China.

The simply constructed fluorescent sensor with inexpensive reagents and low toxicity has attracted increasing attention contributing to its practical application. However, the common construction methods usually required a few building blocks and complex procedures, which is inconvenient for their further application. Herein, a simply constructed fluorescent Hg sensor has been developed based on the intrinsic fluorescence quenching power of G-quadruplex. Two components, AGRO 100 and AMT, were used to construct the sensor. AMT was selected as the fluorescent probe because of its distinct merits. The free AMT emits strongly. However, the fluorescence of AMT could be quenched by G-quadruplex DNA. Additionally, AMT is less toxic and inexpensive. AGRO 100 acts as both the quencher and the capture sequence because it consists of G-rich sequences and T-T mismatched base pairs. The fluorescence of AMT could be quenched by the formed G-quadruplex structure of AGRO 100 in the presence of K. In the presence of Hg, G-quadruplex structure of AGRO 100 was switched to hairpin DNA structure because T-T mismatched base pairs in AGRO 100 could specifically recognize and capture Hg with high affinity. Thus, AMT was released and the fluorescence of AMT was recovered. The developed sensing system was successfully applied to detect Hg in human serum with good recovery and reproducibility.
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http://dx.doi.org/10.1007/s00216-021-03837-zDOI Listing
January 2022

Aptamer-Target Recognition-Promoted Ratiometric Electrochemical Strategy for Evaluating the Microcystin-LR Residue in Fish without Interferences.

J Agric Food Chem 2022 Jan 10. Epub 2022 Jan 10.

College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, People's Republic of China.

Given the significance of food safety, it is highly urgent to develop a sensitive yet reliable sensor for the practical analysis of algal toxins. As most of the developed sensors are disturbed by interfering substances and the target toxin is detected in a single-signal manner based on the immunoassay technology. Herein, we developed an aptamer-based dual-signal ratiometric electrochemical sensor for the sensitive and accurate analysis of microcystin-LR (MC-LR), using it as a proof-of-concept analyte. Methylene blue-tagged ssDNA (MB-ssDNA) was immobilized at the gold electrode surface accompanied with the absence of ferrocene-tagged ssDNA (Fc-ssDNA), resulting in a high differential pulse voltammetry (DPV) current of MB and a low DPV current of Fc. The recognition of MB-ssDNA by MC-LR stimulated the formation of [email protected], which induced the removal of MB-ssDNA from the electrode and the exposure of SH-ssDNA, enabling Fc-ssDNA to be captured at the electrode surface via nucleic acid hybridization. In comparison with MC-LR deficiency, the DPV signal of MB dropped along with an improved DPV signal of Fc, contributing to the ratiometric detection of MC-LR, with the limit of detection down to 0.0015 nM. Furthermore, this ratiometric electrochemical sensor was successfully explored to assess the bioaccumulated amount of MC-LR in the liver and meat of fish. The aptamer-based ratiometric strategy to develop an electrochemical MC-LR assay will offer a promising avenue to develop high-performance sensors, and the sensor will find more useful application in MC-LR-related aquatic product safety studies.
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http://dx.doi.org/10.1021/acs.jafc.1c06476DOI Listing
January 2022

Optical Fiber Bundle-Based High-Speed and Precise Micro-Scanning for Image High-Resolution Reconstruction.

Sensors (Basel) 2021 Dec 25;22(1). Epub 2021 Dec 25.

Key Laboratory on Adaptive Optics, Chinese Academy of Sciences, Chengdu 610209, China.

We propose an imaging method based on optical fiber bundle combined with micro-scanning technique for improving image quality without complex image reconstruction algorithms. In the proposed method, a piezoelectric-ceramic-chip is used as the micro-displacement driver of the optical fiber bundle, which has the advantages of small volume, fast response speed and high precision. The corresponding displacement of the optical fiber bundle can be generated by precise voltage controlling. An optical fiber bundle with core/cladding diameter 4/80 μm and hexagonal arrangement is used to scan the 1951 USAF target. The scanning step is 1 μm, which is equivalent to the diffraction limit resolution of the optical system. The corresponding information is recorded at high speed through photo-detectors and a high-resolution image is obtained by image stitching processing. The minimum distinguishable stripe width of the proposed imaging technique with piezoelectric-ceramic-chip driven micro-scanning is approximately 2.1 μm, which is 1 time higher than that of direct imaging with a CCD camera whose pixel size is close to the fiber core size. The experimental results indicate that the optical fiber bundle combined with piezoelectric-ceramic-chip driven micro-scanning is a high-speed and high-precision technique for high-resolution imaging.
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http://dx.doi.org/10.3390/s22010127DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747347PMC
December 2021

DeepLRR: An Online Webserver for Leucine-Rich-Repeat Containing Protein Characterization Based on Deep Learning.

Plants (Basel) 2022 Jan 4;11(1). Epub 2022 Jan 4.

Key Lab of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.

Members of the leucine-rich repeat (LRR) superfamily play critical roles in multiple biological processes. As the LRR unit sequence is highly variable, accurately predicting the number and location of LRR units in proteins is a highly challenging task in the field of bioinformatics. Existing methods still need to be improved, especially when it comes to similarity-based methods. We introduce our DeepLRR method based on a convolutional neural network (CNN) model and LRR features to predict the number and location of LRR units in proteins. We compared DeepLRR with six existing methods using a dataset containing 572 LRR proteins and it outperformed all of them when it comes to overall F1 score. In addition, DeepLRR has integrated identifying plant disease-resistance proteins (NLR, LRR-RLK, LRR-RLP) and non-canonical domains. With DeepLRR, 223, 191 and 183 LRR-RLK genes in Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa ssp. Japonica) and tomato (Solanum lycopersicum) genomes were re-annotated, respectively. Chromosome mapping and gene cluster analysis revealed that 24.2% (54/223), 29.8% (57/191) and 16.9% (31/183) of LRR-RLK genes formed gene cluster structures in Arabidopsis, rice and tomato, respectively. Finally, we explored the evolutionary relationship and domain composition of LRR-RLK genes in each plant and distributions of known receptor and co-receptor pairs. This provides a new perspective for the identification of potential receptors and co-receptors.
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http://dx.doi.org/10.3390/plants11010136DOI Listing
January 2022

Advances and prospects on the aquatic plant coupled with sediment microbial fuel cell system.

Environ Pollut 2022 Jan 8;297:118771. Epub 2022 Jan 8.

Beijing Key Laboratory for Source Control Technology of Water Pollution, Engineering Research Center for Water Pollution Source Control and Eco-remediation, Beijing Forestry University, Beijing, 100083, China. Electronic address:

Energy resource scarcity and sediment pollution perniciousness have become enormous challenges, to which research has been focused on energy recovery and recycle technologies to solve both above problems. The organic matter stored in anoxic sediments of freshwater ecosystem represents a tremendous potential energy source. The system of aquatic plant coupled with sediment microbial fuel cell (AP-SMFC) has attracted much attention as a more feasible, economical and eco-friendly way to remediate sediment and surface water and generate electricity. However, the research on AP-SMFC has only been carried out in the last decade, and relevant studies have not been well summarized. In this review, the advances and prospects on AP-SMFC were systematically introduced. Firstly, the annual publication counts and keywords co-occurrence cluster of AP-SMFC were identified and visualized by resorting to the CiteSpace software, and the result showed that the research on AP-SMFC increased significantly in the last decade on the whole and will continue to increase. The bibliometric results provided valuable references and information on potential research directions for future studies. And then, the research progress and reaction mechanism of AP-SMFC were systematically described. Thirdly, the performance of AP-SMFC, including nutrients removal, organic contaminants removal, and electricity generation, was systematically summarized. AP-SMFC can enhance the removal of pollutants and electricity generation compared with SMFC without AP, and is considered to be an ideal technology for pollutants removal and resource recovery. Finally, the current challenges and future perspectives were summarized and prospected. Therefore, the review could serve as a guide for the new entrants to the field and further development of AP-SMFC application.
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http://dx.doi.org/10.1016/j.envpol.2021.118771DOI Listing
January 2022

Causes Intracranial Infection: A Case Study.

Front Med (Lausanne) 2021 24;8:761924. Epub 2021 Dec 24.

Department of Emergency Medicine, West China Hospital, Sichuan University, Chengdu, China.

is a rarely encountered bacterium in clinical practice. It is a rare gram-negative rod-shaped bacterium associated with lung and urinary tract infections, but never found in cerebrospinal fluid. This paper reports a case of an adult patient infected by via an unknown route of infection causing a severe intracranial infection. was detected by culture and Metagenomic next generation sequencing in CSF. Early identification of this strain and treatment with sensitive antibiotics is necessary to reduce morbidity and mortality. A 24-year-old male was admitted to a West China Hospital because of headache and vomiting for 2 months. Symptom features included acute onset and long duration of illness. Notably, headache and vomiting were the primary neurological symptoms. Routine cerebrospinal fluid culture failed to identify the bacterium; however, bacterium was detected via second-generation sequencing techniques. was found to be a multi-drug resistant organism, hence, treatment with ceftriaxone, a commonly used drug for intracranial infections was ineffective. This strain eventually caused severe intracranial infection resulting in the death of the patient. In summary, this study comprehensively describes a case of an adult patient infected by and discusses its early identification as well as application of sensitive antibiotics in the emergency setting.
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http://dx.doi.org/10.3389/fmed.2021.761924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739271PMC
December 2021

Case Report: Respiratory Management With a 47-Day ECMO Support for a Critical Patient With COVID-19.

Front Med (Lausanne) 2021 24;8:714387. Epub 2021 Dec 24.

Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

This paper reports a complete case of severe acute respiratory distress syndrome (ARDS) caused by coronavirus disease 2019 (COVID-19), who presented with rapid deterioration of oxygenation during hospitalization despite escalating high-flow nasal cannulation to invasive mechanical ventilation. After inefficacy with lung-protective ventilation, positive end-expiratory pressure (PEEP) titration, prone position, we administered extracorporeal membrane oxygenation (ECMO) as a salvage respiratory support with ultra-protective ventilation for 47 days and finally discharged the patient home with a good quality of life with a Barthel Index Score of 100 after 76 days of hospitalization. The purpose of this paper is to provide a clinical reference for the management of ECMO and respiratory strategy of critical patients with COVID-19-related ARDS.
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http://dx.doi.org/10.3389/fmed.2021.714387DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739513PMC
December 2021

Comparison of first-generation EGFR-TKIs (gefitinib, erlotinib, and icotinib) as adjuvant therapy in resected NSCLC patients with sensitive EGFR mutations.

Transl Lung Cancer Res 2021 Nov;10(11):4120-4129

Department of Thoracic Surgery and Oncology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Background: Several randomized controlled trials have suggested that adjuvant epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) were associated with prolonged disease-free survival (DFS) in EGFR-mutated NSCLC patients after radical resection, comparing with chemotherapy or placebo. We aimed to compare the effectiveness of different first-generation EGFR-TKIs as adjuvant treatment in real-world setting.

Methods: Early-stage EGFR mutated NSCLC patients who underwent radical resection and treated with first-generation EGFR-TKIs (gefitinib, erlotinib, icotinib) as adjuvant therapy between Feb 2010 and Jan 2019 were retrieved from a prospectively-maintained database in our center. The primary endpoint was DFS in stage II/III (TNM 8th) patients with exploratory endpoint regarding DFS in stage I patients. Sensitivity analyses were based on propensity score matched (PSM) cohorts. Treatment failure patterns among different TKIs were also compared.

Results: Of 588 eligible patients, 198 patients (33.7%) received gefitinib, 106 patients (17.9%) received erlotinib, and 284 patients (48.2%) received icotinib. The median DFS of stage II/III patients in the gefitinib, erlotinib and icotinib group were 36.1 months (95% CI, 23.9-49.4), 42.8 months (95% CI, 29.6-97.8), and 32.5 months (95% CI, 23.9-49.4), respectively, with no significant difference (log-rank test P=0.22). There was also no significant difference in DFS among stage I patients receiving different TKIs (P=0.12). PSM adjustments and multivariate analyses adjusting for other confounders revealed similar results. In addition, there were no significant differences in treatment failure pattens in different EGFR-TKI arms, especially in terms of brain metastases (6.1% in gefitinb, 7.5% in erlotinib, 3.9% in icotinib) and bone metastases (8.6% in gefitinb, 9.4% in erlotinib, 7.0% in icotinib).

Conclusions: This first and largest real-world study showed that gefitinib, erlotinib, and icotinib demonstrated comparable clinical effectiveness as adjuvant therapy for patients with early-stage EGFR mutated NSCLC.
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http://dx.doi.org/10.21037/tlcr-21-649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8674600PMC
November 2021

The Role of Unfolded Protein Response in Coronavirus Infection and Its Implications for Drug Design.

Authors:
Mei Xue Li Feng

Front Microbiol 2021 24;12:808593. Epub 2021 Dec 24.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Harbin, China.

Coronavirus is an important pathogen with a wide spectrum of infection and potential threats to humans and animals. Its replication occurs in the cytoplasm and is closely related to the endoplasmic reticulum (ER). Studies reported that coronavirus infection causes ER stress, and cells simultaneously initiate unfolded protein response (UPR) to alleviate the disturbance of ER homeostasis. Activation of the three branches of UPR (PERK, IRE1, and ATF6) modulates various signaling pathways, such as innate immune response, microRNA, autophagy, and apoptosis. Therefore, a comprehensive understanding of the relationship between coronavirus and ER stress is helpful to understand the replication and pathogenesis of coronavirus. This paper summarizes the current knowledge of the complex interplay between coronavirus and UPR branches, focuses on the effect of ER stress on coronavirus replication and coronavirus resistance to host innate immunity, and summarizes possible drug targets to regulate the impact of coronavirus infection.
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http://dx.doi.org/10.3389/fmicb.2021.808593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8740020PMC
December 2021

Retrograde Recanalization of Occluded Radial Artery: A Single-Centre Experience and Literature Review.

J Endovasc Ther 2022 Jan 10:15266028211067732. Epub 2022 Jan 10.

Department of Cardiology, Wujin Hospital Affiliated with Jiangsu University, The Wujin Clinical College of Xuzhou Medical University, Changzhou, China.

Purpose: Radial artery occlusion (RAO) is one of the common complications after coronary intervention via the conventional radial artery approach. The purpose of the study was to explore the safety and feasibility of retrograde recanalization of the occluded radial artery via a distal radial artery (DRA) approach.

Methods: Combined with the practice of our centre and a literature review, we summarized the procedure of retrograde recanalization of RAO, success rate, and complications.

Results: A total of 14 of 15 patients with 15 pieces of occluded radial arteries were successfully recanalized via the DRA in our centre. In the 15 occluded vessels, 11 vessels (73.3%) had total occlusion and 4 vessels (26.7%) had functional occlusion. Four of 15 occluded vessels were acute occlusions. Two acute RAOs were only treated with aspiration via sheath, 11 RAOs with balloon angioplasty, and 2 RAOs with both, respectively. In 6 patients, cardiac catheterization was carried out via the DRA after recanalizing the RAO. A total of 10 studies reporting the results of recanalization of RAO via the DRA were systematically retrieved in the present study. In 3 case series, the number of cases was more than 5, and the success rate of recanalization was more than 85.7%. Two studies reported complications, including dissection in one case, hematoma in 2 cases, and pain in the forearm during angioplasty.

Conclusions: Recanalization of the occluded radial artery via the DRA was safe and effective. When repeat cardiac catheterization was required, recanalization of the RAO and subsequent coronary angiography or intervention through the ipsilateral radial artery approach was feasible.
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http://dx.doi.org/10.1177/15266028211067732DOI Listing
January 2022

Blood biomarkers associated with acute type II respiratory failure in COPD: A meta-analysis.

Authors:
Tieying Shi Li Feng

Clin Respir J 2022 Jan 10. Epub 2022 Jan 10.

Department of Geriatrics, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.

Objective: This study aims to summarize the risk factors of type II respiratory failure in patients with an acute exacerbation of chronic obstructive pulmonary disease (COPD), to guide clinical treatment in time, and consequently reduce the serious impact of COPD on human health.

Methods: Five databases were searched for relevant articles on risk factors of acute exacerbation of COPD combinate with type II respiratory failure. We calculated the standard mean difference (SMD), odds ratio (OR), and their 95% confidence interval (95% CI) utilizing a fixed-effect model or a random-effect model according to the level of heterogeneity.

Results: As of 14 May 2021, 13 articles were included in our meta-analysis. The results showed that low albumin and uric acid levels were the risk factors for type II respiratory failure in acute exacerbation of COPD patients, and the differences were statistically significant (albumin: SMD = -2.03, 95% CI: -2.81, -1.26; uric acid: SMD = -1.28, 95% CI: -1.41, -1.15). Besides, 10 other systematic markers have been reported to be the risk factors for type II respiratory failure of patients with acute exacerbation of COPD, but only in single study.

Conclusion: The meta-analysis results further confirm that low albumin and uric acid levels are risk factors for type II respiratory failure in acute exacerbation of COPD patients. Additionally, this analysis also summarizes many emerging inflammatory indicators, nutritional indicator, and cardiovascular system indicators to predict the progression of acute exacerbation of COPD to type II respiratory failure but only in single study.
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http://dx.doi.org/10.1111/crj.13464DOI Listing
January 2022

Estimation of the capillary level input function for dynamic contrast-enhanced MRI of the breast using a deep learning approach.

Magn Reson Med 2022 Jan 9. Epub 2022 Jan 9.

Department of Radiology, Weill Cornell Medical College, New York, New York, USA.

Purpose: To develop a deep learning approach to estimate the local capillary-level input function (CIF) for pharmacokinetic model analysis of DCE-MRI.

Methods: A deep convolutional network was trained with numerically simulated data to estimate the CIF. The trained network was tested using simulated lesion data and used to estimate voxel-wise CIF for pharmacokinetic model analysis of breast DCE-MRI data using an abbreviated protocol from women with malignant (n = 25) and benign (n = 28) lesions. The estimated parameters were used to build a logistic regression model to detect the malignancy.

Result: The pharmacokinetic parameters estimated using the network-predicted CIF from our breast DCE data showed significant differences between the malignant and benign groups for all parameters. Testing the diagnostic performance with the estimated parameters, the conventional approach with arterial input function (AIF) showed an area under the curve (AUC) between 0.76 and 0.87, and the proposed approach with CIF demonstrated similar performance with an AUC between 0.79 and 0.81.

Conclusion: This study shows the feasibility of estimating voxel-wise CIF using a deep neural network. The proposed approach could eliminate the need to measure AIF manually without compromising the diagnostic performance to detect the malignancy in the clinical setting.
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http://dx.doi.org/10.1002/mrm.29148DOI Listing
January 2022

WTAP-mediated mA modification of lncRNA DIAPH1-AS1 enhances its stability to facilitate nasopharyngeal carcinoma growth and metastasis.

Cell Death Differ 2022 Jan 8. Epub 2022 Jan 8.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat-sen University Cancer Center, 510060, Guangzhou, P.R. China.

As the most predominant RNA epigenetic regulation in eukaryotic cells, N-methyladenosine (mA) plays a critical role in human tumorigenesis and cancer progression. However, the biological function and molecular mechanism of mA regulation in naso-pharyngeal carcinoma (NPC) remain elusive. Here, we showed that Wilms' tumor 1-associating protein (WTAP) expression was apparently upregulated in NPC, and increased WTAP was associated with poor prognosis. WTAP upregulated in NPC was fine-tuned by KAT3A-mediated H3K27 acetylation. Functionally, WTAP was required for the growth and metastasis of NPC. Mechanistically, lncRNA DIAPH1-AS1 was identified as a bona fide mA target of WTAP. WTAP-mediated mA modification of DIAPH1-AS1 enhanced its stability relying on the mA reader IGF2BP2-dependent pathway. Furthermore, DIAPH1-AS1 acted as a molecular adaptor that promoted MTDH-LASP1 complex formation and upregulated LASP1 expression, ultimately facilitating NPC growth and metastasis. Thus, WTAP-mediated DIAPH1-AS1 mA methylation is required for NPC tumorigenesis and metastasis.
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http://dx.doi.org/10.1038/s41418-021-00905-wDOI Listing
January 2022

Structural insight into the alginate derived nano-La(OH)/porous carbon composites for highly selective adsorption of phosphate.

Int J Biol Macromol 2022 Jan 4;200:172-181. Epub 2022 Jan 4.

Chongqing Key Laboratory of Catalysis and New Environmental Materials, College of Environment and Resources, Chongqing Technology and Business University, Chongqing 400067, China.

In this study, a novel nano-La(OH)/porous carbon composites derived from La alginate xerogel with egg-box structure had been successfully synthesized by a gradient heat treatment in nitrogen atmosphere. This facile fabrication strategy can be easily employed to considerably encapsulate La(OH) nanoparticles uniformly into the porous carbon matrix derived from the alginate macromolecule framework. The optimized sample, labeled as LS-550(N), exhibited extremely high phosphate uptake and great selectivity. The adsorption kinetic process dramatically followed pseudo-second-order model. The Langmuir model fitted maximum equilibrium adsorption capacity is 133.58 mg·g. The phosphate adsorption mechanisms could be consist of electrostatic interaction, complexation and ligand exchange interaction on the surface of LS-550(N). The prominent practical applicability of LS-550(N) in the regeneration test suggests that the LS-550(N) could be a potential adsorption candidate for the decontamination of phosphate-containing natural water bodies.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.12.076DOI Listing
January 2022

Efficient Role of Nanosheet-Like PrO Induced Surface-Interface Synergistic Structures over Cu-Based Catalysts for Enhanced Methanol Production from CO Hydrogenation.

ACS Appl Mater Interfaces 2022 Jan 7. Epub 2022 Jan 7.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.

In a complex heterogeneous metal-catalyzed reaction process, unique cooperative effects between metal sites and surface-interface active sites, as well as favorable synergy between surface-interface active sites, can play crucial roles in improving their catalytic performances. In this work, a ZnO-modified Cu-based catalyst over defect-rich PrO nanosheets for high-efficiency CO hydrogenation to produce methanol was successfully constructed. It was demonstrated that an as-fabricated nanosheet-like Cu-based catalyst presented several structural advantages including the formation of highly dispersive Cu sites and the coexistence of abundant defective Pr-V-Pr structures (V: oxygen vacancy) and interfacial Cu-O-Pr sites. Combining structural characterization and catalytic reaction results with density functional theory calculations, it was clearly unveiled that the synergy between surface defective structures and Cu-PrO interfaces over the catalyst remarkably promoted the adsorption of CO and CO intermediate, thus boosting the CO hydrogenation simultaneously both the formate intermediate pathway and the intense reverse water-gas shift reaction-derived CO hydrogenation pathway, along with a high space-time yield of methanol of 0.395 g·g·h under mild reaction conditions (260 °C and 3.0 MPa). The study provides a new strategy to construct high-performance Cu-based catalysts for high-efficiency CO hydrogenation to produce methanol and a deep understanding of the promotional roles of synergy between surface-interface active sites in the CO hydrogenation.
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http://dx.doi.org/10.1021/acsami.1c20056DOI Listing
January 2022

The effects of health behaviours and beliefs based on message framing among patients with chronic diseases: a systematic review.

BMJ Open 2022 Jan 6;12(1):e055329. Epub 2022 Jan 6.

School of Nursing, Jilin University, Changchun, Jilin, China

Objective: The effectiveness of integrating message framing into educational interventions to promote the health behaviour of patients with chronic diseases is still being debated in nursing research. The objective of this study was to assess the impact of educational interventions based on gain and loss frames on the health behaviours and beliefs of patients with chronic diseases and to identify the frame that achieves better outcomes.

Design: The systematic review was based on PRISMA guidelines for comprehensively searching, appraising and synthesising research evidence.

Data Sources: We searched the PubMed, Web of Science, PsycINFO and CINAHL databases for reports published from database inception until 26 March 2021.

Eligibility Criteria: Intervention studies, published in English, with adult patients with chronic disease conditions, and with intervention contents involved in the implementation of message framing, were considered. The outcomes were health behaviours or beliefs, such as knowledge, self-efficacy, intention or attitudes.

Data Extraction And Synthesis: Data extraction and entry were performed using a predesigned data extraction form and assessed independently by two reviewers using the Cochrane Collaboration Risk of Bias I.

Results: A total of 11 intervention studies were included. We found that educational intervention based on both gain and loss frames could enhance the positive effects of communication, and promote healthy behaviours and beliefs in patients with chronic disease. Many of the studies we included here showed the advantage of loss framing messages. Due to the limited number of articles included and without quantitative analysis, this result should be interpreted cautiously.

Conclusions: Integrating message framing into health education might be a promising strategy to motivate patients with chronic disease to improve their health behaviours and beliefs. More extensive and well-designed trials are needed to support the conclusions and discuss the effective framing, moderators and mediators of framing.

Prospero Registration Number: CRD42021250931.
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http://dx.doi.org/10.1136/bmjopen-2021-055329DOI Listing
January 2022

MicroRNA-20a-5p Inhibits the Autophagy and Cisplatin Resistance in Ovarian Cancer via Regulating DNMT3B-mediated DNA Methylation of RBP1.

Reprod Toxicol 2022 Jan 3. Epub 2022 Jan 3.

Gynaecology and Obstetrics, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, 030010, Shanxi, China. Electronic address:

Ovarian cancer (OvCa) is the third most common female malignancy worldwide and poses great threats on women health. Chemotherapy is the most recommended post-surgery treatment for OvCa patients; but, cisplatin resistance is a main cause of chemotherapy failure. In addition, autophagy modulates the sensitivity of tumor cells to chemotherapeutic agents. Hence, it is significant to explore the molecular mechanism concerning the autophagy and cisplatin resistance in OvCa. In this study, quantitative real-time PCR (qRT-PCR) was used to detect miR-20a-5p expression and western blot to measure RBP1 expression. A series of assays were conducted to explore the gain-of-function effects of miR-20a-5p. Luciferase reporter assay was applied to determine the downstream target of miR-20a-5p. The results proved that miR-20a-5p represses malignant phenotypes and autophagy in cisplatin-resistant OvCa cells. In addition, DNMT3B mediates DNA methylation of RBP1 to impair the promoting effects of RBP1 on carcinogenesis and autophagy in OvCa. Through rescue experiments, we certified that miR-20a-5p inhibits the autophagy and cisplatin resistance in OvCa via DNMT3B-mediated DNA methylation of RBP1. Collectively, we demonstrated that miR-20a-5p plays a crucial role in the modulation of autophagy and cisplatin resistance in OvCa, which might offer novel insights into developing effective treatment strategies for OvCa.
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http://dx.doi.org/10.1016/j.reprotox.2021.12.011DOI Listing
January 2022
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