Publications by authors named "Li Chen"

7,910 Publications

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Upregulation of METTL14 contributes to trophoblast dysfunction by elevating FOXO3a expression in an mA-dependent manner.

Placenta 2022 May 14;124:18-27. Epub 2022 May 14.

Department of Reproductive Medicine Center, Changzhou Maternal and Child Health Care Hospital, Changzhou Medical Center, Nanjing Medical University, Changzhou, 213000, China. Electronic address:

Introduction: Preeclampsia, a specific complication of pregnancy, is a leading cause of perinatal and maternal mortality worldwide. N-methyladenosine (mA) is a prevalent and reversible modification of mammalian mRNAs, and is known to play an important role in various physiological and pathological processes. However, little is known about its possible effects on trophoblasts in preeclampsia.

Methods: Colorimetric RNA mA methylation quantification assay and dot blotting were used to assess the levels of global RNA mA modification in placental tissues collected from females with normal pregnancy and preeclampsia, while the mRNA levels of major mA methyltransferases/demethylases were investigated by quantitative real-time polymerase chain reaction. The effects of methyltransferase-like 14 (METTL14) on trophoblasts were evaluated using cell counting kit-8, transwell invasion assay, autophagic flux assay, and Annexin V/propidium iodide apoptosis assay. The molecular mechanism underlying the regulation of forkhead box O3a (FOXO3a) expression by METTL14 was determined using methylated RNA immunoprecipitation and transcription inhibition assays.

Results: Global RNA mA methylation and METTL14 expression were significantly increased in placental tissues obtained from patients with preeclampsia. In vitro studies showed that overexpression of METTL14 in HTR-8/SVneo cells inhibited trophoblast proliferation and invasion, but induced trophoblast autophagy and apoptosis. We further demonstrated that METTL14 epigenetically elevated FOXO3a expression via an mA-dependent mechanism. FOXO3a inhibition effectively prevented the impairment of trophoblast proliferation and invasion, and diminished the induction of trophoblast autophagy and apoptosis in METTL14-overexpressing HTR-8/SVneo cells.

Discussion: Increased METTL14-mediated mA modification results in an adverse impact on trophoblast function by elevating FOXO3a expression.
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http://dx.doi.org/10.1016/j.placenta.2022.05.008DOI Listing
May 2022

Respiratory viruses among pediatric inpatients with acute lower respiratory tract infections in Jinan, China, 2016-2019.

J Med Virol 2022 May 20. Epub 2022 May 20.

Department of Clinical Laboratory, Shandong Provincial Hospital affiliated to Shandong First Medical University, Jinan, Shandong, PR China.

The viral etiologies responsible for acute lower respiratory tract infections (ALRI) are a major cause of pediatric hospitalization, and some develop severe diseases requiring pediatric intensive care unit (PICU) admission. The aim of this study was to determine the prevalence of viruses and risk factors associated with PICU admission among patients hospitalized for ALRI. Nasopharyngeal swabs were collected to detect human rhinovirus (HRV), influenza A and B viruses (IAV, IBV), parainfluenza viruses (PIV), and respiratory syncytial virus (RSV) by reverse transcription-polymerase chain reaction (RT-PCR) and adenovirus (ADV) by PCR. Of the 5590 pediatric inpatients enrolled, respiratory viral infection occurred in 2102 (37.60%) patients, including 1846 (33.02%) single and 256 (4.58%) mixed viral infections. Among the nasopharyngeal swabs from pediatric inpatients, HRV accounted for the highest detection rate (16.48%), followed by PIV (8.35%), RSV (7.41%), ADV (4.63%), IAV (3.51%), and IBV (2.08%). The positive rate of viral tests decreased with increasing age and was higher in males (39.29%) than females (34.67%). The prevalence of viral infection was the highest in winter (41.57%) and lowest in autumn (31.78%). Each virus had a seasonal pattern, with peaks occurring in months of their epidemic seasons. RSV infection and the presence of comorbidities including congenital tracheal stenosis, congenital heart disease, metabolic disorder, immunodeficiency, renal disease, gastrointestinal disease and neurological disorder might be associated with the need for PICU admission. Therefore, this study provides useful information for the prevention and control of virus-related respiratory diseases and the early identification of and the intervention in severe cases. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/jmv.27875DOI Listing
May 2022

AKG Attenuates Cerebral Ischemia-Reperfusion Injury through c-Fos/IL-10/Stat3 Signaling Pathway.

Oxid Med Cell Longev 2022 10;2022:6839385. Epub 2022 May 10.

Neurovascular Center, Changhai Hospital, Naval Medical University, Shanghai, China.

Inflammation is dominant in the pathogenesis of ischemic stroke (IS). Alpha-ketoglutarate (AKG), according to previous studies, has demonstrated a variety of pharmacological effects such as antioxidation and inhibitive inflammation activities. However, whether AKG ameliorates cerebral ischemic injury, as well as the underlying molecular events, is still unclear. Therefore, the effect and underlying mechanisms of AKG on ischemic brain injury should be identified. The study established a cerebral ischemia-reperfusion (I/R) model in mice as well as an oxygen-glucose deprivation/reperfusion (OGD/R) model in SH-SY5Y cells, respectively. It was observed that AKG markedly suppressed infarction volume and neuronal injuries and improved the neurological score in vivo. Moreover, AKG reduced the inflammatory response and lowered the expression of proinflammatory cytokines. In vitro, AKG treatment strongly inhibited OGD/R-induced neuronal injury and the proinflammatory factors. It was also found that the increased SOD and GSH levels, as well as the lower ROS levels, showed that AKG reduced oxidative stress in OGD/R-treated SY-SY5Y cells. Mechanistically, AKG largely promoted IL-10 expression in ischemic brain injury and OGD/R-induced neuronal injury. Furthermore, IL-10 silencing neutralized the protective effect of AKG on inflammation. Notably, it was discovered that AKG could upregulate IL-10 expression by promoting the translocation of c-Fos from the cytoplasm to the nucleus. The results indicated that AKG demonstrated neuroprotection on cerebral ischemia while inhibiting inflammation through c-Fos/IL-10/stat3 pathway.
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http://dx.doi.org/10.1155/2022/6839385DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113869PMC
May 2022

The Interaction Between rs2071345 Polymorphism and Alcohol Dependence in Anxiety Symptoms Among Chinese Male Problem Drinkers.

Front Psychiatry 2022 3;13:878960. Epub 2022 May 3.

School of Mental Health, Wenzhou Medical University, Wenzhou, China.

Objective: Alcohol dependence can increase the level of anxiety. A growing body of research has identified a link between anxiety symptoms of problem drinkers and their genetic or environment factors, respectively. However, to date few studies have directly examined gene-environment (G × E) interaction on their anxiety symptoms during the acute alcohol withdrawal. The present study aims to examine the interaction between the proopiomelanocortin () rs2071345 polymorphism and alcohol dependence on anxiety symptoms of male problem drinkers, and further test the exact form of interaction on two competing models: the diathesis-stress model vs. the differential susceptibility model.

Methods: A total of 440 male problem drinkers ( = 44.5 years, = 9.45) were recruited from nine main psychiatric hospitals of northern China during acute alcohol withdrawal. Blood samples were collected for genotyping, self-reported anxiety symptoms, and levels of alcohol dependence were assessed.

Results: Results indicated that the rs2071345 polymorphism significantly moderated anxiety symptoms associated with alcohol dependence. A region of significance (RoS) test showed that male problem drinkers with T allele were more likely to experience more anxiety symptoms than those with CC homozygote when the standardized score of concurrent alcohol dependence was above 0.31. Confirmatory model evaluation indicated that the interaction effect involving gene polymorphism conformed to the diathesis-stress model rather than differential-susceptibility model of person × environment interaction.

Conclusions: This study suggested that the SNP in rs2071345 was associated with alcohol dependence in anxiety symptoms of male problem drinkers and further provided evidence in support of the diathesis-stress hypothesis of alcohol dependence in terms of anxiety symptoms.
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http://dx.doi.org/10.3389/fpsyt.2022.878960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110641PMC
May 2022

Single Cell Raman Spectroscopy Deuterium Isotope Probing for Rapid Antimicrobial Susceptibility Test of spp.

Front Microbiol 2022 3;13:876925. Epub 2022 May 3.

School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou, China.

Nosocomial infection by multi-drug resistance spp. is an emerging concern with severe clinical consequences, particularly in immunocompromised individuals and infants. Efficient control of this infection requires quick and reliable methods to determine the appropriate drugs for treatment. In this study, a total of 31 spp., including two standard strains (ATCC 13253 and FMS-007) and 29 clinical isolates obtained from hospitals in China were subjected to single cell Raman spectroscopy analysis coupled with deuterium probing (single cell Raman-DIP). The results demonstrated that single cell Raman-DIP could determine antimicrobial susceptibility of spp. in 4 h, only one third of the time required by standard broth microdilution method. The method could be integrated into current clinical protocol for sepsis and halve the report time. The study also confirmed that minocycline and levofloxacin are the first-line antimicrobials for spp. infection.
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http://dx.doi.org/10.3389/fmicb.2022.876925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113537PMC
May 2022

Automatic Implementation Algorithm of Pressure Relief Drilling Depth Based on an Innovative Monitoring-While-Drilling Method.

Sensors (Basel) 2022 Apr 22;22(9). Epub 2022 Apr 22.

School of Energy and Mining Engineering, China University of Mining and Technology (Beijing), Beijing 100083, China.

An innovative monitoring-while-drilling method of pressure relief drilling was proposed in a previous study, and the periodic appearance of amplitude concentrated enlargement zone in vibration signals can represent the drilling depth. However, there is a lack of a high accuracy model to automatically identify the amplitude concentrated enlargement zone. So, in this study, a neural network model is put forward based on single-sensor and multi-sensor prediction results. The neural network model consists of one Deep Neural Network (DNN) and four Long Short-Term Memory (LSTM) networks. The accuracy is only 92.72% when only using single-sensor data for identification, while the proposed multiple neural network model could improve the accuracy to being greater than 97.00%. In addition, an optimization method was supplemented to eliminate some misjudgment due to data anomalies, which improved the final accuracy to the level of manual recognition. Finally, the research results solved the difficult problem of identifying the amplitude concentrated enlargement zone and provided the foundation for automatically identifying the drilling depth.
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http://dx.doi.org/10.3390/s22093234DOI Listing
April 2022

Role of Ions in Hydrogels with an Ionic Seebeck Coefficient of 52.9 mV K.

J Phys Chem Lett 2022 May 19:4621-4627. Epub 2022 May 19.

MOE Key Laboratory of Low-grade Energy Utilization Technologies and Systems, CQU-NUS Renewable Energy Materials & Devices Joint Laboratory, School of Energy & Power Engineering, Chongqing University, Chongqing 400044, China.

Ionic thermoelectric (i-TE) material with mobile ions as charge carriers has the potential to generate large thermal voltages at low operating temperatures. This study highlights the role of ions in i-TE hydrogels employing a poly(vinyl alcohol) (PVA) polymer matrix and a number of ion providers, e.g., KOH, KNO, KCl, KBr, NaI, KI, and CsI. The relationship between the intrinsic physical parameters of the ion and the thermoelectric performance is established, indicating the ability to influence the hydrogen bond by the ion is a crucial factor. Among these i-TE hydrogels, the PVA/CsI hydrogel exhibits the largest ionic Seebeck coefficient, reaching 52.9 mV K, which is the largest of all i-TE materials reported to date. In addition, our work demonstrates the influence of ions on polymer configuration and provides an avenue for ion selection in the Soret effect in ionic thermoelectrics.
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http://dx.doi.org/10.1021/acs.jpclett.2c00845DOI Listing
May 2022

 (Primulaceae), a new species from Yunnan, China.

PhytoKeys 2022 11;194:15-22. Epub 2022 Apr 11.

Forestry and Grassland Bureau, Lincang 677000, Yunnan, China Forestry and Grassland Bureau Lincang China.

from SW Yunnan, China, is described as a species new to science and illustrated. The systematic placement of this new species is also discussed based on an nrITS molecular tree. It is morphologically most similar to , but differs from the latter in its racemose inflorescence, green calyx tube, pink to pink rose corolla, stamens at 1/3 length above the base of the corolla tube and applanate globose capsule.
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http://dx.doi.org/10.3897/phytokeys.194.81335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9016029PMC
April 2022

The impact of blastomere loss on pregnancy and neonatal outcomes of vitrified-warmed Day3 embryos in single embryo transfer cycles.

J Ovarian Res 2022 May 18;15(1):62. Epub 2022 May 18.

Department of Assisted Reproduction, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, China.

Background: Blastomere loss is a common phenomenon that occurs following cryopreservation. To date, studies have drawn conflicting conclusions regarding the impact of blastomere loss on pregnancy outcomes. Besides, limited information is available concerning the neonatal safety of embryos with blastomere loss. In the present study, we aimed to investigate the impact of blastomere loss on pregnancy and neonatal outcomes of vitrified/warmed Day3 cleavage-stage embryos in single embryo transfer cycles.

Methods: This retrospective cohort study included all vitrified/warmed D3 cleavage-stage single frozen-thawed embryo transfer (FET) cycles between April 2015 and February 2021. We compared pregnancy and subsequent neonatal outcomes between the intact embryos group and the blastomere loss group in single FET cycles.

Results: A total of 6287 single FET cycles were included in the study, in which 5873 cycles were classified into the intact embryo group and 414 cycles were classified into the blastomere loss group. The outcomes of the blastomere loss group were significantly inferior to those of the intact embryo group, in terms of implantation/biochemical pregnancy/clinical pregnancy/ongoing pregnancy rate and live birth rate per embryo transfer cycle/per clinical pregnancy. Further binary logistic regression confirmed that blastomere loss was negatively associated with live birth. Moreover, the blastomere loss group presented with an elevated early miscarriage rate. The neonatal conditions were broadly similar between the two groups. Additionally, multiple binary logistic regression analysis demonstrated that primary infertility and intracytoplasmic sperm injection (ICSI) were common influencing factors of blastomere loss (aOR 1.447, 95% CI 1.038-2.019, P = 0.029; aOR: 1.388, 95% CI: 1.044-51.846, P = 0.024).

Conclusions: The transfer of vitrified/warmed D3 embryos with blastomere loss is related to impaired embryo developmental potentials and reduced probabilities of conception. Moreover, even if the embryos with blastomere loss have implanted and reached clinical pregnancies, they present with a lower possibility of developing to live birth owing to a higher early miscarriage rate. However, once the embryos with blastomere loss result in a live birth, no adverse neonatal outcomes are observed. Primary infertility and ICSI were found to be risk factors for blastomere loss.
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http://dx.doi.org/10.1186/s13048-022-00997-zDOI Listing
May 2022

Preparation of 2-Methoxyestradiol Self-emulsified Drug Delivery System and the Effect on Combination Therapy with Doxorubicin Against MCF-7/ADM Cells.

AAPS PharmSciTech 2022 May 18;23(5):147. Epub 2022 May 18.

Department of Pharmaceutical Sciences, School of Basic Medical Sciences, Henan University of Science and Technology, Luoyang, 471023, Henan, People's Republic of China.

Due to the poor solubility and bioavailability of 2-methoxyestradiol (2-ME), 2-ME emulsified drug delivery system (2-ME-SEDDS) was designed and characterized. After dilution with 5% glucose, 2-ME-SEDDS formed fine emulsions with mean diameter of 171 ± 14 nm and zeta potential of - 7.4 ± 0.6 mV. The cytotoxicity of 2-ME-SEDDS against MCF-7 and MCF-7/ADM cells was considerable to that of free 2-ME, and the half maximal inhibitory concentration ran up to 195 µg/mL on MCF-7/ADM cells. In order to gain a satisfactory inhibition effect on MCF-7/ADM cells, 2-ME-SEDDS combined with doxorubicin was used. It is worth noting that the combination of 2-ME-SEDDS and doxorubicin displayed a superior synergistic effect with a combined index of 0.62. And the cellular uptake of doxorubicin by MCF-7/ADM cells in the combination group was significantly higher than that of doxorubicin treatment group. The study preliminarily suggested that 2-ME-SEDDS could increase the cellular uptake of doxorubicin by MCF-7/ADM cells and the synergistic effect may be attributed to the increased cellular uptake of doxorubicin under the influence of 2-ME-SEDDS. In conclusion, SEDDS was an alternative and promising formulation for 2-ME. The combination therapy with synergistic effect by the combination of 2-ME-SEDDS and doxorubicin seems to be a promising strategy to potentiate anti-tumor efficiency against MCF-7/ADM, even other multidrug resistance tumors.
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http://dx.doi.org/10.1208/s12249-022-02298-6DOI Listing
May 2022

Measurement of 7-dehydrocholesterol and cholesterol in hair can be used in the diagnosis of Smith-Lemli-Opitz syndrome.

J Lipid Res 2022 May 13:100228. Epub 2022 May 13.

Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, PR China. Electronic address:

7-dehydrocholesterol (7-DHC) and cholesterol (CHOL) are biomarkers of Smith-Lemli-Opitz Syndrome (SLOS), a congenital autosomal recessive disorder characterized by elevated 7-DHC level in patients. Hair samples have been shown to have great diagnostic and research value, which has long been neglected in the SLOS field. In this study, we sought to investigate the feasibility of using hair for SLOS diagnosis. In the presence of antioxidants (2,6-ditert-butyl-4-methylphenol and triphenylphosphine), hair samples were completely pulverized and extracted by micro-pulverized extraction (MPE) in alkaline solution or in n-hexane. After microwave-assisted derivatization (MAD) with N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA), the analytes were measured by gas chromatography-mass spectrometry (GC-MS). We found that the limits of determination (LOD) for 7-DHC and CHOL were 10 ng/mg and 8 ng/mg, respectively. In addition, good linearity was obtained in the range of 50-4000 ng/mg and 30-6000 ng/mg for 7-DHC and CHOL, respectively, which fully meets the requirement for SLOS diagnosis and related research. Finally, by applying the proposed method to real hair samples collected from 14 healthy infants and 2 suspected SLOS patients, we confirmed the feasibility of hair analysis as a diagnostic tool for SLOS. In conclusion, we present an optimized and validated analytical method for the simultaneous determination of two SLOS biomarkers using human hair.
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http://dx.doi.org/10.1016/j.jlr.2022.100228DOI Listing
May 2022

Pantothenate protects against obesity via brown adipose tissue activation.

Am J Physiol Endocrinol Metab 2022 May 16. Epub 2022 May 16.

Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Brown adipose tissue (BAT) is the primary site of adaptive thermogenesis, which is involved in energy expenditure and has received much attention in the field of obesity treatment. By screening a small-molecular library of Food and Drug Administration approved drugs, pantothenic acid was identified as being able to significantly upregulate the expression of uncoupling protein 1 (UCP1), a key thermogenic protein found in BAT. Pantothenate (PA) treatment decreased adiposity, reversed fatty liver and improved glucose homeostasis via increased energy expenditure in C57BL/6J mice fed a high-fat diet. PA could also increase BAT activity and induce beige formation. Mechanistically, the beneficial effects were evidently mediated by UCP1, because PA treatment was unable to ameliorate the obesity in UCP1 knockout mice. In conclusion, we identified PA as a safe and effective BAT activator that can reduce obesity and may represent a promising strategy for the clinical treatment of obesity and related metabolic diseases.
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http://dx.doi.org/10.1152/ajpendo.00293.2021DOI Listing
May 2022

[Bone marrow mesenchymal stem cells attenuate pyroptosis lipopolysaccharide-induced renal injury rats].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2022 Mar;34(3):284-288

Department of Intensive Care Unit, the Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan, China. Corresponding author: Chen Li, Email:

Objective: To investigate the effect and mechanism of bone marrow mesenchymal stem cell (BMSC) on pyroptosis of rats with kidney injury.

Methods: Bone marrow of 4-5 week-old female Sprague-Dawley (SD) rats was isolated in vitro and BMSC was obtained. The third generations of BMSC were used to further experiments. Fifteen 6 week-old SD rats were cluster-randomized divided into control group, kidney injury group and BMSC group (5 rats in each group). Rats in kidney injury group were injected with lipopolysaccharide (LPS) 1 mg/kg via tail vein; the control group was given the same amount of normal saline. BMSC group was injected with 0.5 mL BMSC (including 2×10 BMSC) via tail vein after modeling; the kidney injury group received the same amount of normal saline. On day 3 after these injections, serum creatinine (SCr) was detected by picric acid method, and blood urea nitrogen (BUN) was detected by diacetyl monoxime. The levels of cystatin C (Cys C), interleukins (IL-1β and IL-18) in blood were detected by enzyme-linked immunosorbent assay (ELISA). The rats were then sacrificed and their kidneys were removed for subsequent detection. The mRNA expression levels of NOD-like receptor protein 3 (NLRP3) and cysteinyl aspartate-specific protease-1 (caspase-1) of kidney were detected by quantificational real-time quantitative polymerase chain reaction (qRT-PCR). The protein expression levels of NLRP3 and caspase-1 of kidney were detected by Western blotting.

Results: In vitro, after bone marrow cell suspension was cultured for 24 hours, a large number of round adherent cells and suspended cells appeared in each culture flask. After 4-5 days of culture, a large number of long spindle cells adhered to the wall, and there were still obvious impurity cells. After trypsin digestion and passage to the third generation, the long spindle adherent cells grew mainly in the culture flask and were basically purified as BMSC. In vivo, compared with the control group, the levels of SCr, BUN, Cys C, IL-1β and IL-18 in kidney injury group were increased [SCr (μmol/L): 85.22±2.29 vs. 21.80±0.59, BUN (mmol/L): 11.50±0.64 vs. 5.86±0.83, Cys C (mg/L): 0.13±0.01 vs. 0.11±0.02, IL-1β (ng/L): 31.49±1.42 vs. 4.74±0.49, IL-18 (ng/L): 29.01±1.95 vs. 1.52±0.03, all P < 0.05]. The mRNA and protein expression levels of NLRP3, caspase-1 were significantly increased [NLRP3 mRNA (2): 3.635±0.296 vs. 1.000±0.002, caspase-1 mRNA (2): 4.020±0.228 vs. 1.001±0.003; NLRP3 protein (NLRP3/β-actin): 1.560±0.868 vs. 0.902±0.036, caspase-1 protein (caspase-1/β-actin): 1.392±0.097 vs. 0.895±0.046, all P < 0.05]. Compared with kidney injury group, the levels of SCr, BUN, IL-1β and IL-18 in BMSC group were significantly decreased [SCr (μmol/L): 51.64±3.84 vs. 85.22±2.29, BUN (mmol/L): 9.90±0.46 vs. 11.50±0.64, IL-1β (ng/L): 24.20±1.45 vs. 31.49±1.42, IL-18 (ng/L): 12.97±1.25 vs. 29.01±1.95, all P < 0.05]. The mRNA and protein expression levels of NLRP3, caspase-1 were significantly decreased [NLRP3 mRNA (2): 1.488±0.136 vs. 3.635±0.296, caspase-1 mRNA (2): 1.643±0.143 vs. 4.020±0.228; NLRP3 protein (NLRP3/β-actin): 1.227±0.053 vs. 1.560±0.868, caspase-1 protein (caspase-1/β-actin): 1.159±0.107 vs. 1.392±0.097, all P < 0.05].

Conclusions: In vivo, BMSC may attenuate pyroptosis in LPS-induced kidney injury rats.
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http://dx.doi.org/10.3760/cma.j.cn121430-20211117-01735DOI Listing
March 2022

Deep Learning-Based Identification of Maize Leaf Diseases Is Improved by an Attention Mechanism: Self-Attention.

Front Plant Sci 2022 28;13:864486. Epub 2022 Apr 28.

School of Information and Computer, Anhui Agricultural University, Hefei, China.

Maize leaf diseases significantly reduce maize yield; therefore, monitoring and identifying the diseases during the growing season are crucial. Some of the current studies are based on images with simple backgrounds, and the realistic field settings are full of background noise, making this task challenging. We collected low-cost red, green, and blue (RGB) images from our experimental fields and public dataset, and they contain a total of four categories, namely, southern corn leaf blight (SCLB), gray leaf spot (GLS), southern corn rust (SR), and healthy (H). This article proposes a model different from convolutional neural networks (CNNs) based on transformer and self-attention. It represents visual information of local regions of images by tokens, calculates the correlation (called attention) of information between local regions with an attention mechanism, and finally integrates global information to make the classification. The results show that our model achieves the best performance compared to five mainstream CNNs at a meager computational cost, and the attention mechanism plays an extremely important role. The disease lesions information was effectively emphasized, and the background noise was suppressed. The proposed model is more suitable for fine-grained maize leaf disease identification in a complex background, and we demonstrated this idea from three perspectives, namely, theoretical, experimental, and visualization.
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http://dx.doi.org/10.3389/fpls.2022.864486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096888PMC
April 2022

Non-convulsive Status Epilepticus in -Related Progressive Myoclonic Epilepsy: A Case Report With Literature Review.

Front Pediatr 2022 28;10:859183. Epub 2022 Apr 28.

Department of Neurology, Shenzhen Children's Hospital, Shenzhen, China.

Progressive myoclonic epilepsy (PME) is a group of rare diseases characterized by progressive myoclonus, cognitive impairment, ataxia, and other neurologic deficits. PME has high genetic heterogeneity, and more than 40 genes are reportedly associated with this disorder. encodes a member of the semaphorin family and was first reported to cause PME in 2020. Herein, we present a rare case of PME due to a novel gene mutation in a 6-year-old boy born to healthy non-consanguineous Chinese parents. His developmental milestones were delayed, and he developed recurrent atonic seizures and myoclonic seizures without fever at 3 years and 11 months of age. He experienced recurrent myoclonic seizures, non-convulsive status epilepticus (NCSE), atonic seizures, and atypical absence seizures during the last 2 years. At different time points since onset, valproic acid, levetiracetam, piracetam, and clobazam were used to control the intractable seizures. Notably, NCSE was controlled by a combination of piracetam with clobazam and valproic acid instead of intravenous infusion of midazolam and phenobarbital. Due to the limited number of cases reported to date, the clinical description of our case provides a better understanding of the genotype-phenotype correlations associated with PME and indicate that piracetam may be effective against NCSE in patients with -related PME.
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http://dx.doi.org/10.3389/fped.2022.859183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096209PMC
April 2022

CRISPR/Cas9-Mediated Knockout of the and Genes in BHK-21 Cell Promoted Seneca Virus A Replication and Enhanced Autophagy.

Front Cell Infect Microbiol 2022 27;12:865744. Epub 2022 Apr 27.

Key Laboratory of Animal Resistance Biology of Shandong Province, College of Life Science, Shandong Normal University, Jinan, China.

RNA interference (RNAi) is a major form of antiviral defense in host cells, and Ago2 and Dicer are the major proteins of RNAi. The Senecavirus A (SVA) is a reemerging virus, resulting in vesicular lesions in sows and a sharp decline in neonatal piglet production. In this study, CRISPR/Cas9 technology was used to knock out Ago2 and Dicer genes in BHK-21 cell lines used for SVA vaccine production. Cell clones with homozygous frameshift mutations of Ago2 and Dicer genes were successfully identified. The two knockout cell lines were named BHK-Dicer and BHK-Ago2. Results showed that the two genes' knockout cell lines were capable of stable passage and the cell growth rate did not change significantly. The replication rate and virus titers of SVA were significantly increased in knockout cell lines, indicating that RNAi could inhibit SVA replication. In addition, compared with normal cells, autophagy was significantly enhanced after SVA-infected knockout cell lines, while there was no significant difference in autophagy between the knockout and normal cell lines without SVA. The results confirmed that SVA could enhance the autophagy in knockout cells and promote viral replication. The two knockout cell lines can obtain viruses with high viral titers and have good application prospects in the production of SVA vaccine. At the same time, the RNAi knockout cell lines provide convenience for further studies on RNAi and SVA resistance to RNAi, and it lays a foundation for further study of SVA infection characteristics and screening of new therapeutic drugs and drug targets.
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http://dx.doi.org/10.3389/fcimb.2022.865744DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9093602PMC
May 2022

A novel nomogram for predicting cancer-specific survival in women with uterine sarcoma: a large population-based study.

BMC Womens Health 2022 May 14;22(1):175. Epub 2022 May 14.

Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.

Background: Uterine sarcoma (US) is a rare malignant uterine tumor with aggressive behavior and rapid progression. The purpose of this study was to constructa comprehensive nomogram to predict cancer-specific survival (CSS) of patients with US-based on the Surveillance, Epidemiology, and End Results (SEER) database.

Methods: A retrospective population-based study was conducted using data from patients with US between 2010 and 2015 from the SEER database. They were randomly divided into a training cohort and a validation cohort ata 7-to-3 ratio. Multivariate Cox analysis was performed to identify independent prognostic factors. Subsequently, a nomogram was established to predict patient CSS. The discrimination and calibration of the nomogram were evaluated by the concordance index (C-index) and the area under the curve (AUC). Finally, net reclassification improvement (NRI), integrated discrimination improvement (IDI), calibration plotting, and decision-curve analysis (DCA) were used to evaluate the benefits of the new prediction model.

Results: A total of 3861 patients with US were included in our study. As revealed in multivariate Cox analysis, age at diagnosis, race, marital status, insurance record, tumor size, pathology grade, histological type, SEER stage, AJCC stage, surgery status, radiotherapy status, and chemotherapy status were found to be independent prognostic factors. In our nomogram, pathology grade had strongest correlation with CSS, followed by age at diagnosis and surgery status. Compared to the AJCC staging system, the new nomogram showed better predictive discrimination with a higher C-index in the training and validation cohorts (0.796 and 0.767 vs. 0.706 and 0.713, respectively). Furthermore, the AUC value, calibration plotting, NRI, IDI, and DCA also demonstrated better performance than the traditional system.

Conclusion: Our study validated the first comprehensive nomogram for US, which could provide more accurate and individualized survival predictions for US patients in clinical practice.
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http://dx.doi.org/10.1186/s12905-022-01739-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107666PMC
May 2022

Urolithin A protects human dermal fibroblasts from UVA-induced photoaging through NRF2 activation and mitophagy.

J Photochem Photobiol B 2022 May 11;232:112462. Epub 2022 May 11.

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, PR China. Electronic address:

Photoaging, caused by exposure to sunlight and especially UVA, has been identified as one of the culprits for age-related skin deterioration. Here, we initially demonstrated that urolithin A (UroA), a metabolite derived from intestine microflora, possessed sufficient photoprotective capacity and attenuated UVA-induced senescent phenotypes in human fibroblasts, such as growth inhibition, senescence-associated β-galactosidase activity, breakdown of extracellular matrix, synthesis of senescence-associated secretory phenotypes and cell cycle arrest. Furthermore, UroA lessened the accumulation of intracellular reactive oxygen species, which promoted the phosphorylation and afterwards nuclear translocation of NRF2, subsequently driving the activation of downstream antioxidative enzymes. In parallel, we proved that UroA restored mitochondrial function by induction of mitophagy, which was regulated by the SIRT3-FOXO3-PINK1-PARKIN network. Taken together, our results showed that UroA protected dermal fibroblast from UVA damage through NRF2/ARE activation and mitophagy process, thus supporting UroA as a potential therapeutic agent for photoaging.
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http://dx.doi.org/10.1016/j.jphotobiol.2022.112462DOI Listing
May 2022

Virus-like siRNA construct dynamically responsive to sequential microenvironments for potent RNA interference.

J Colloid Interface Sci 2022 May 5;622:938-949. Epub 2022 May 5.

Department of Gastric Cancer, Cancer Hospital of Dalian University of Technology (Liaoning Cancer Hospital & Institute), Shenyang, Liaoning 110042, China. Electronic address:

Cytoplasmic transportation of therapeutic nucleic acids is deemed as an onerous task with aim of precise knockdown towards the targeted genes. Pertaining to the programed functionalities of natural virus in circumventing the biological barriers, we tailored multifaceted chemistries into manufacture of synthetic siRNA delivery vehicles in resembling the functionalities of viral vectors to dynamically tackle with a sequential of biological obstacles encountered in the journey of systemic anti-tumor RNAi therapy. Once harnessing ligands with RGD motif for specific internalization into subcellular endosomal compartments of the tumor cells, the architecture of the proposed delivery vehicles was subjected to facile transformation responsive to pH stimuli in acidic endosomal compartments. The external biocompatible PEGylation palisade was consequently detached, unveiling the cytomembrane-lytic cationic components to commit disruptive potencies to the anionic endosomal membranes for translocation of siRNA conjugates into cytosol. Eventually, liberation of active siRNA could be accomplished due to its responsiveness to the strikingly high level of glutathione in cytosol, thereby contributing to potent RNAi. Hence, our elaborated virus-mimicking platform has demonstrated significant anti-tumor efficacy through systemic administration of anti-angiogenic RNAi payloads, which inspired prosperous potentials in a variety of therapeutic applications.
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http://dx.doi.org/10.1016/j.jcis.2022.05.006DOI Listing
May 2022

Delivery Methods of Cognitive Behavior Therapy for Patients With Irritable Bowel Syndrome.

Gastroenterol Nurs 2022 May 6. Epub 2022 May 6.

Li Juen Chen, MS, RN, is PhD Candidate, Biobehavioral Nursing and Health Systems, School of Nursing, University of Washington, Seattle; UW Medicine Valley Medical Center, Renton, Washington.

Irritable bowel syndrome (IBS) is the most commonly diagnosed gastrointestinal disorder and negatively impacts individuals' quality of life. Cognitive behavioral therapy appears effective for reducing symptoms in many irritable bowel syndrome patients. However, the optimal methods to deliver cognitive behavioral therapy and the effective treatment dosage for patients with IBS remain unclear. This article aims to provide an update on cognitive behavioral therapy research in IBS, particularly considering the dose of treatment, route of delivery (in-person vs. web- and telephone-based delivery), and outcome measures. A systematic literature review was conducted using databases of PubMed, CINAHL Complete, and Web of Science from 2008 through 2021. Twelve studies reporting randomized clinical trials comparing cognitive behavioral therapy delivered with in-person, telephone, and web for the management of IBS symptoms among adults with irritable bowel syndrome were found. The dose of treatment varied from 4 to 10 sessions. Six different scales measured various outcomes. No severe adverse reactions to cognitive behavioral therapy were reported. Cognitive behavioral therapy is an effective treatment for IBS symptoms regardless of the dose and the route of treatment. However, it is difficult to compare the effectiveness of these randomized clinical trials due to the various cognitive behavioral therapy protocols, combined routes of therapy delivery, and different outcome measures used.
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http://dx.doi.org/10.1097/SGA.0000000000000671DOI Listing
May 2022

Construction of Bio-inspired Film with Engineered Hydrophobicity to Boost Interfacial Reaction Kinetics of Aqueous Zinc-Ion Batteries.

Small 2022 May 13:e2201732. Epub 2022 May 13.

MOE Key Laboratory of Low-grade Energy Utilization Technologies and Systems, CQU-NUS Renewable Energy Materials & Devices Joint Laboratory, School of Energy & Power Engineering, Chongqing University, Chongqing, 400044, P. R. China.

Aqueous zinc-ion batteries typically suffer from sluggish interfacial reaction kinetics and drastic cathode dissolution owing to the desolvation process of hydrated Zn and continual adsorption/desorption behavior of water molecules, respectively. To address these obstacles, a bio-inspired approach, which exploits the moderate metabolic energy of cell systems and the amphiphilic nature of plasma membranes, is employed to construct a bio-inspired hydrophobic conductive poly(3,4-ethylenedioxythiophene) film decorating α-MnO cathode. Like plasma membranes, the bio-inspired film can "selectively" boost Zn migration with a lower energy barrier and maintain the integrity of the entire cathode. Electrochemical reaction kinetics analysis and theoretical calculations reveal that the bio-inspired film can significantly improve the electrical conductivity of the electrode, endow the cathode-electrolyte interface with engineered hydrophobicity, and enhance the desolvation behavior of hydrated Zn . This results in an enhanced ion diffusion rate and minimized cathode dissolution, thereby boosting the overall interfacial reaction kinetics and cathode stability. Owing to these intriguing merits, the composite cathode can demonstrate remarkable cycling stability and rate performance in comparison with the pristine MnO cathode. Based on the bio-inspired design philosophy, this work can provide a novel insight for future research on promoting the interfacial reaction kinetics and electrode stability for various battery systems.
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http://dx.doi.org/10.1002/smll.202201732DOI Listing
May 2022

Flexible and high-performance piezoresistive strain sensors based on multi-walled carbon [email protected] foam.

RSC Adv 2022 May 11;12(22):14190-14196. Epub 2022 May 11.

School of Textile Science and Engineering, Tiangong University Tianjin 300387 China

Flexible wearable pressure sensors have attracted special attention in the last 10 years due to their great potential in health monitoring, activity detection and as electronic skin. However, it is still a great challenge to develop high sensitivity, fast response, and good reliable stability through a simple and reproducible large-scale fabrication process. Here, we develop a simple and efficient method to fabricate three-dimensional (3D) light-weight piezoresistive sensing materials by coating multi-walled carbon nanotubes (MWCNTs) on the surface of polyurethane (PU) foam using a dip-spin coating process. The PU foam prepared with SEBS-g-MAH and polyether polyols has high elasticity and good stability in MWCNTs/DMF solution. Subsequently, a piezoresistive sensor was assembled with the prepared MWCNTs/PU composite foam and copper foil electrodes. The assembled pressure sensor has high sensitivity (62.37 kPa), a wide working range (0-172.6 kPa, 80% strain), a fast response time (less than 0.6 s), and reliable repeatability (≥2000 cycles). It has shown potential application in real-time human motion detection (, arm bending, knee bending), and monitoring the brightness of LED lights.
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http://dx.doi.org/10.1039/d2ra01291jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092363PMC
May 2022

TIRSF: a web server for screening gene signatures to predict Tumor immunotherapy response.

Nucleic Acids Res 2022 May 12. Epub 2022 May 12.

School of Life Sciences, Institute of Precision Medicine, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510060, China.

Immune checkpoint blockade (ICB) therapy has been successfully applied to clinically therapeutics in multiple cancers, but its efficacy varies greatly among different patients and cancer types. Therefore, the construction of gene signatures to identify patients who could benefit from ICB therapy is particularly important for precision cancer treatment. However, due to the lack of a user-friendly platform, the construction of such gene signatures is a great challenge for clinical investigators who have limited programming skills. In light of this challenge, we developed a web server called Tumor Immunotherapy Response Signature Finder(TIRSF) for the construction of gene signatures to predict ICB therapy response in cancer patients. TIRSF consists of three functional modules. The first module is the Signature Discovery module which provides signature construction and performance evaluation functionalities. The second is a module for response prediction based on the TIRSF signatures, which enables response prediction and prognostic analysis of immunotherapy samples. The last is a module for response prediction based on existing signatures. This module currently integrates 24 published signatures for ICB therapy response prediction. Together, all of above features can be freely accessed at http://tirsf.renlab.org/.
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http://dx.doi.org/10.1093/nar/gkac374DOI Listing
May 2022

Enhancement of Electrochromic Properties of Polyaniline Induced by Copper Ions.

Nanoscale Res Lett 2022 May 12;17(1):51. Epub 2022 May 12.

School of Physics and Electronics, Central South University, Changsha, 410083, China.

Driven by the urgent need for adaptive infrared (IR) electrochromic devices, the improvement in electrochromic performance based on polyaniline (PANI) conducting polymers has become an outstanding challenge. In recent years, the acid doping strategy has been proven to increase the IR modulation ability of PANI, in particular for the Bronsted acid doping. Herein, the effects of copper ions, a Lewis acid, on the structure and electrochromic properties of polyaniline were investigated. Compared to pure polyaniline, the Cu-doped PANI porous films show better IR modulation ability. With the increasing concentration of copper ions, the Cu-doped PANI porous films exhibit a trend in volcanic patterns for the emittance variation (∆ε), depending on the number of polarons and bipolarons. The optimal IR emissivity (ε) modulation obtained on Cu-doped PANI films shows the ∆ε modulation of 0.35 and 0.3 in the wavelength range of 8-14 µm and 2.5-25 µm, superior to previously reported pure sulfuric acid-doped PANI. Furthermore, a flexible IR electrochromic device was fabricated with the present Cu-doped PANI porous films. The modulation of the emittance variation varied between 0.513 and 0.834 (∆ε = 0.32 in ranges of wavelength 8-12 µm), suggesting the great potential for applications in military camouflage and intelligent IR thermal management. We believe that the results in this work will provide a novel perspective and avenue for improving the IR modulation ability of electrochromic devices based on polyaniline conducting polymers.
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http://dx.doi.org/10.1186/s11671-022-03689-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098743PMC
May 2022

Translocon-associated protein subunit SSR3 determines and predicts susceptibility to paclitaxel in breast cancer and glioblastoma.

Clin Cancer Res 2022 May 12. Epub 2022 May 12.

Northwestern University, Chicago, Illinois, United States.

Purpose: Paclitaxel (PTX) is one the most potent and commonly used chemotherapies for breast and pancreatic cancer. Several ongoing clinical trials are investigating means of enhancing delivery of PTX across the blood-brain barrier for glioblastomas (GBMs). Despite the widespread use of PTX for breast cancer, and the initiative to repurpose this drug for gliomas, there are no predictive biomarkers to inform which patients will likely benefit from this therapy.

Experimental Design: To identify predictive biomarkers for susceptibility to PTX, we performed a genome-wide CRISPR knock-out (KO) screen using human glioma cells. The genes whose KO was most enriched in the CRISPR screen underwent further selection based on their correlation with survival in the breast cancer patient cohorts treated with PTX and not in patients treated with other chemotherapies, a finding that was validated on a second independent patient cohort using progression-free survival.

Results: Combination of CRISPR screen results with outcomes from taxane-treated breast cancer patients led to the discovery of endoplasmic reticulum (ER) protein SSR3 as a putative predictive biomarker for PTX. SSR3 protein levels showed positive correlation with susceptibility to PTX in breast cancer cells, glioma cells and in multiple intracranial glioma xenografts models. Knockout of SSR3 turned the cells resistant to PTX while its overexpression sensitized the cells to PTX. Mechanistically, SSR3 confers susceptibility to PTX through regulation of phosphorylation of ER stress sensor IRE1α.

Conclusion: Our hypothesis generating study showed SSR3 as a putative biomarker for susceptibility to PTX, warranting its prospective clinical validation.
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http://dx.doi.org/10.1158/1078-0432.CCR-21-2563DOI Listing
May 2022

Dorzagliatin in drug-naïve patients with type 2 diabetes: a randomized, double-blind, placebo-controlled phase 3 trial.

Nat Med 2022 May 12;28(5):965-973. Epub 2022 May 12.

The Second Affiliated Hospital of Hainan Medical University, Haikou, China.

Improving glucose sensitivity remains an unmet medical need in treating type 2 diabetes (T2D). Dorzagliatin is a dual-acting, orally bioavailable glucokinase activator that enhances glucokinase activity in a glucose-dependent manner, improves glucose-stimulated insulin secretion and demonstrates effects on glycemic control in patients with T2D. We report the findings of a randomized, double-blind, placebo-controlled phase 3 clinical trial to evaluate the efficacy and safety of dorzagliatin in patients with T2D. Eligible drug-naïve patients with T2D (n = 463) were randomly assigned to the dorzagliatin or placebo group at a ratio of 2:1 for 24 weeks of double-blind treatment, followed by 28 weeks of open-label treatment with dorzagliatin for all patients. The primary efficacy endpoint was the change in glycated hemoglobin from baseline to week 24. Safety was assessed throughout the trial. At week 24, the least-squares mean change in glycated hemoglobin from baseline (95% confidence interval) was -1.07% (-1.19%, -0.95%) in the dorzagliatin group and -0.50% (-0.68%, -0.32%) in the placebo group (estimated treatment difference, -0.57%; 95% confidence interval: -0.79%, -0.36%; P < 0.001). The incidence of adverse events was similar between the two groups. There were no severe hypoglycemia events or drug-related serious adverse events in the dorzagliatin group. In summary, dorzagliatin improved glycemic control in drug-naïve patients with T2D and showed a good tolerability and safety profile.
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http://dx.doi.org/10.1038/s41591-022-01802-6DOI Listing
May 2022

Dorzagliatin add-on therapy to metformin in patients with type 2 diabetes: a randomized, double-blind, placebo-controlled phase 3 trial.

Nat Med 2022 May 12;28(5):974-981. Epub 2022 May 12.

Shanghai Pudong New Area People's Hospital, Shanghai, China.

Metformin, the first-line therapy for type 2 diabetes (T2D), decreases hepatic glucose production and reduces fasting plasma glucose levels. Dorzagliatin, a dual-acting orally bioavailable glucokinase activator targeting both the pancreas and liver glucokinase, decreases postprandial glucose in patients with T2D. In this randomized, double-blind, placebo-controlled phase 3 trial, the efficacy and safety of dorzagliatin as an add-on therapy to metformin were assessed in patients with T2D who had inadequate glycemic control using metformin alone. Eligible patients with T2D (n = 767) were randomly assigned to receive dorzagliatin or placebo (1:1 ratio) as an add-on to metformin (1,500 mg per day) for 24 weeks of double-blind treatment, followed by 28 weeks of open-label treatment with dorzagliatin for all patients. The primary efficacy endpoint was the change in glycated hemoglobin (HbA1c) levels from baseline to week 24, and safety was assessed throughout the trial. At week 24, the least-squares mean change from baseline in HbA1c (95% confidence interval (CI)) was -1.02% (-1.11, -0.93) in the dorzagliatin group and -0.36% (-0.45, -0.26) in the placebo group (estimated treatment difference, -0.66%; 95% CI: -0.79, -0.53; P < 0.0001). The incidence of adverse events was similar between groups. There were no severe hypoglycemia events or drug-related serious adverse events in the dorzagliatin and metformin combined therapy group. In patients with T2D who experienced inadequate glycemic control with metformin alone, dorzagliatin resulted in effective glycemic control with good tolerability and safety profile ( NCT03141073 ).
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http://dx.doi.org/10.1038/s41591-022-01803-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9117147PMC
May 2022

FER-mediated phosphorylation and PIK3R2 recruitment on IRS4 promotes AKT activation and tumorigenesis in ovarian cancer cells.

Elife 2022 May 12;11. Epub 2022 May 12.

School of Life Science and Technology, ShanghaiTech University, Shanghai, China.

Tyrosine phosphorylation, orchestrated by tyrosine kinases and phosphatases, modulates a multi-layered signaling network in a time- and space-dependent manner. Dysregulation of this post-translational modification is inevitably associated with pathological diseases. Our previous work has demonstrated that non-receptor tyrosine kinase FER is upregulated in ovarian cancer, knocking down which attenuates metastatic phenotypes. However, due to the limited number of known substrates in the ovarian cancer context, the molecular basis for its pro-proliferation activity remains enigmatic. Here, we employed mass spectrometry and biochemical approaches to identify insulin receptor substrate 4 (IRS4) as a novel substrate of FER. FER engaged its kinase domain to associate with the PH and PTB domains of IRS4. Using a proximity-based tagging system in ovarian carcinoma-derived OVCAR-5 cells, we determined that FER-mediated phosphorylation of Tyr779 enables IRS4 to recruit PIK3R2/p85β, the regulatory subunit of PI3K, and activate the PI3K-AKT pathway. Rescuing -null ovarian tumor cells with phosphorylation-defective mutant, but not WT IRS4 delayed ovarian tumor cell proliferation both in vitro and in vivo. Overall, we revealed a kinase-substrate mode between FER and IRS4, and the pharmacological inhibition of FER kinase may be beneficial for ovarian cancer patients with PI3K-AKT hyperactivation.
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http://dx.doi.org/10.7554/eLife.76183DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098222PMC
May 2022

Pro-Arrhythmic Effects of Discontinuous Conduction at the Purkinje Fiber-Ventricle Junction Arising From Heart Failure-Induced Ionic Remodeling - Insights From Computational Modelling.

Front Physiol 2022 25;13:877428. Epub 2022 Apr 25.

Biological Physics Group, Department of Physics and Astronomy, The University of Manchester, Manchester, United Kingdom.

Heart failure is associated with electrical remodeling of the electrical properties and kinetics of the ion channels and transporters that are responsible for cardiac action potentials. However, it is still unclear whether heart failure-induced ionic remodeling can affect the conduction of excitation waves at the Purkinje fiber-ventricle junction contributing to pro-arrhythmic effects of heart failure, as the complexity of the heart impedes a detailed experimental analysis. The aim of this study was to employ computational models to investigate the pro-arrhythmic effects of heart failure-induced ionic remodeling on the cardiac action potentials and excitation wave conduction at the Purkinje fiber-ventricle junction. Single cell models of canine Purkinje fiber and ventricular myocytes were developed for control and heart failure. These single cell models were then incorporated into one-dimensional strand and three-dimensional wedge models to investigate the effects of heart failure-induced remodeling on propagation of action potentials in Purkinje fiber and ventricular tissue and at the Purkinje fiber-ventricle junction. This revealed that heart failure-induced ionic remodeling of Purkinje fiber and ventricular tissue reduced conduction safety and increased tissue vulnerability to the genesis of the unidirectional conduction block. This was marked at the Purkinje fiber-ventricle junction, forming a potential substrate for the genesis of conduction failure that led to re-entry. This study provides new insights into proarrhythmic consequences of heart failure-induced ionic remodeling.
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http://dx.doi.org/10.3389/fphys.2022.877428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9081695PMC
April 2022

EMDS-6: Environmental Microorganism Image Dataset Sixth Version for Image Denoising, Segmentation, Feature Extraction, Classification, and Detection Method Evaluation.

Front Microbiol 2022 25;13:829027. Epub 2022 Apr 25.

Institute of Medical Informatics, University of Lübeck, Lübeck, Germany.

Environmental microorganisms (EMs) are ubiquitous around us and have an important impact on the survival and development of human society. However, the high standards and strict requirements for the preparation of environmental microorganism (EM) data have led to the insufficient of existing related datasets, not to mention the datasets with ground truth (GT) images. This problem seriously affects the progress of related experiments. Therefore, This study develops the (EMDS-6), which contains 21 types of EMs. Each type of EM contains 40 original and 40 GT images, in total 1680 EM images. In this study, in order to test the effectiveness of EMDS-6. We choose the classic algorithms of image processing methods such as image denoising, image segmentation and object detection. The experimental result shows that EMDS-6 can be used to evaluate the performance of image denoising, image segmentation, image feature extraction, image classification, and object detection methods. EMDS-6 is available at the https://figshare.com/articles/dataset/EMDS6/17125025/1.
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http://dx.doi.org/10.3389/fmicb.2022.829027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083104PMC
April 2022
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