Publications by authors named "Li Cao"

1,019 Publications

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The Effects of Rural Residents' Sleep Quality on the Incidence of Agricultural Injuries: A Meta-Analysis.

Contrast Media Mol Imaging 2022 13;2022:3832535. Epub 2022 Jul 13.

Department of Mathematical Statistics, International School of Public Health and One Health, Hainan Medical University, Haikou 571199, China.

To collect relevant literature on the impact of rural residents' sleep quality on the incidence of agricultural injuries, the search time range is 1990~2019. We use RevMan 5.3 software for statistical processing. A total of 7 articles were included. Meta-analysis showed that sleep quality was closely related to agricultural injury. The combined effect was 1.49, 95% [1.31, 1.70],  = 5.93,  < 0.00001. The difference was statistically significant, so poor sleepers had a higher incidence of agricultural injuries than good sleepers. Sleep disturbances and agricultural injuries are two common and significant health problems. Investigations suggest that sleep might increase the risk of agricultural injuries. The aim of the present study was to systematically review and meta-analyze the predictive effect of sleep on agricultural injuries.
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http://dx.doi.org/10.1155/2022/3832535DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9300269PMC
August 2022

Current Occupational Perspective of Total Hip Joint Surgeons in China: A Survey of Members of the Chinese Orthopedic Association.

Orthop Surg 2022 Aug 1. Epub 2022 Aug 1.

Department of Bone and Joint Surgery, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Objectives: To explore the basic occupational information and the preferences of surgical techniques in Chinese joint surgeons.

Methods: A survey study was conducted during the Chinese Hip Society (CHS) conference of 2020. Participants from hospitals all over the country were included as the potential respondents. The questions were asked for respondents' basic information, overviews of total hip arthroplasty (THA), surgical techniques, and prosthesis selection. After data collection and filtration, analysis was conducted using chi-square test or Fisher's exact test.

Results: Only 8.31% of joint surgeons used robotics or navigation systems to assisted their total hip arthroplasty. For the approach preference, posterolateral approaches were generally favored, being used commonly by 75.36% of respondents while the direct anterior approach was considered the preferred choice by 8.31% of Chinese joint surgeons. 24.36% of the respondents choose to use domestic prosthesis in over 80% of their patients.

Conclusions: The intelligent THA is in the early stage at present but developing rapidly in China. The composition of surgical approach is simple at present and the continuing education should aim at increasing the diversity of approach selection in the following years. Domestic prosthesis is playing significant role in the prosthesis market. Joint registration system is urgently needed for Chinese joint surgery.
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http://dx.doi.org/10.1111/os.13405DOI Listing
August 2022

Factors contributing to 1-year dissatisfaction after total knee arthroplasty: a nomogram prediction model.

J Orthop Surg Res 2022 Jul 28;17(1):367. Epub 2022 Jul 28.

Department of Orthopedics, First Affiliated Hospital of Xinjiang Medical University, 137th South LiYuShan Road, Urumqi, 830054, Xinjiang, China.

Background: Identifying risk factors and early intervention are critical for improving the satisfaction rate of total knee arthroplasty (TKA). Our study aimed to identify patient-specific variables and establish a nomogram model to predict dissatisfaction at 1 year after TKA.

Methods: This prospective cohort study involved 208 consecutive primary TKA patients with end-stage arthritis who completed self-reported measures preoperatively and at 1 year postoperatively. All participants were randomized into a training cohort (n = 154) and validation cohort (n = 54). Multiple regression models with preoperative and postoperative factors were used to establish the nomogram model for dissatisfaction at 1 year postoperatively. The least absolute shrinkage and selection operator method was used to screen the suitable and effective risk factors (demographic variables, preoperative variables, surgical variable, and postoperative variables) collected. These variables were compared between the satisfied and dissatisfied groups in the training cohort. The receiver operating characteristic (ROC) curve, calibration plot, and decision curve analysis were used to validate the discrimination, calibration, and clinical usefulness of the model. Results were evaluated by internal validation of the validation cohort.

Results: The overall satisfaction rate 1 year after TKA was 77.8%. The nomogram prediction model included the following risk factors: gender; primary diagnosis; postoperative residual pain; poor postoperative range of motion; wound healing; and the rate of change in the degree of coronal lower limb alignment (hip-knee-ankle angle, HKA).The ROC curves of the training and validation cohorts were 0.9206 (95% confidence interval [CI], 0.8785-0.9627) and 0.9662 (0.9231, 1.0000) (95% CI, 0.9231, 1.0000), respectively. The Hosmer-Lemeshow test showed good calibration of the nomogram (training cohort, p = 0.218; validation cohort, p = 0.103).

Conclusion: This study developed a prediction nomogram model based on partially modifiable risk factors for predicting dissatisfaction 1 year after TKA. This model demonstrated good discriminative capacity for identifying those at greatest risk for dissatisfaction and may help surgeons and patients identify and evaluate the risk factors for dissatisfaction and optimize TKA outcomes.
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http://dx.doi.org/10.1186/s13018-022-03205-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330701PMC
July 2022

Accelerating PERx Reaction Enables Covalent Nanobodies for Potent Neutralization of SARS-Cov-2 and Variants.

Chem 2022 Jul 18. Epub 2022 Jul 18.

Department of Pharmaceutical Chemistry and the Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, 94158, USA.

The long-lasting COVID-19 pandemic and increasing SARS-CoV-2 variants demand effective drugs for prophylactics and treatment. Protein-based biologics offer high specificity yet their noncovalent interactions often lead to drug dissociation and incomplete inhibition. Here we developed covalent nanobodies capable of binding with SARS-CoV-2 irreversibly via proximity-enabled reactive therapeutic (PERx) mechanism. A latent bioreactive amino acid FFY was designed and genetically encoded into nanobodies to accelerate PERx reaction rate. In comparison with the noncovalent wildtype nanobody, the FFY-incorporated covalent nanobodies neutralized both wildtype SARS-CoV-2 and its Alpha, Delta, Epsilon, Lambda, and Omicron variants with potency drastically increased. This PERx-enabled covalent nanobody strategy and insights on potency increase can be valuable to developing effective therapeutics for various viral infections.
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http://dx.doi.org/10.1016/j.chempr.2022.07.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288967PMC
July 2022

Dimethyl sulfoxide and ascarosides improve the growth and yields of entomopathogenic nematodes in liquid cultures.

J Invertebr Pathol 2022 Sep 21;193:107800. Epub 2022 Jul 21.

Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, China. Electronic address:

Increasing the infective juvenile (IJ) yields of entomopathogenic nematodes in monoxenic culture systems would reduce their production cost for the market. Ascarosides act as universal nematode pheromones with developmental and behavioral effects of nematodes. Dimethyl sulfoxide (DMSO) is unexpectedly found to enhance the IJ yields of entomopathogenic nematodes on fortified nutrient broth plates. In this study, the influence of selected ascarosides (ascr#7, ascr#9 and ascr#11) and DMSO in three concentrations on the IJ yields of S. carpocapsae All and H. bacteriophora H06 in liquid culture flasks was determined, and the critical development parameters (IJ recovery rate, number of hermaphrodites, number of visible eggs in a hermaphrodite) were examined for H. bacteriophora H06. The results demonstrated that IJ yields were significantly improved in the liquid medium containing 0.01 % DMSO, and 0.02 nM ascr#11 for S. carpocapsae All, and 0.1 % and 0.01 % DMSO and 0.02 pM ascr#11 for H. bacteriophora H06 in proper concentrations. Furthermore, it was discovered that increased recovery rate, hermaphrodite numbers and eggs in the hermaphrodites may contribute to the improved IJ yields of H. bacteriophora H06 in DMSO-supplemented liquid medium. Compared with the control flasks, the IJ yields from the flasks containing 0.01 % DMSO were 15 % and 35 % higher for S. carpocapsae All and H. bacteriophora H06 respectively in 15 days. The cost for ascarosides and DMSO is almost negligible. The results would provide practical technology for low-cost commercial production of these nematodes for pest management program.
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http://dx.doi.org/10.1016/j.jip.2022.107800DOI Listing
September 2022

Effectiveness of virtual reality in cardiac rehabilitation: A systematic review and meta-analysis of randomized controlled trials.

Int J Nurs Stud 2022 Jul 5;133:104323. Epub 2022 Jul 5.

Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, China; School of Public Policy and Management, Tsinghua University, China. Electronic address:

Background: Cardiovascular disease has risen sharply and causes more premature deaths than cancer, while it represents a major economic burden for healthcare systems and impacts patients' quality of life negatively. Virtual reality has captured the attention of researchers in the field of cardiac rehabilitation. However, the efficacy of virtual reality among individuals undergoing cardiac rehabilitation remains inconclusive.

Objective: To appraise research evidence on the effects of virtual reality for individuals undergoing cardiac rehabilitation.

Design: Systematic review and meta-analysis.

Methods: A systematic search of publications was conducted using Pubmed, Embase, Web of science, Cumulative Index to Nursing and Allied Health Literature database (CINAHL), Cochrane Central Register of Controlled trials and Physiotherapy Evidence Database (PEDro) from inception to 15 May 2022, without language restriction. The Cochrane Risk of Bias Tool was used to examine the methodological quality of the included randomized controlled studies. When feasible, a meta-analysis was performed to calculate the pooled effects using Review Manager (Version 5.4). Otherwise, narrative summaries were performed. The Grading of Recommendations Assessment, Development, and Evaluation (GRADE) methodology was used to assess the certainty of the evidence.

Results: A total of ten studies were included. Virtual reality probably increases exercise capacity for individuals undergoing cardiac rehabilitation (the pooled mean difference 49.55, 95% confidence interval 30.59 ~ 68.52, P < 0.00001, moderate-certainty evidence) and might result in a reduction in emotional tension (mean difference -6.43, 95% confidence interval -9.02 ~ -3.84, P < 0.00001, low-certainty evidence) and intrapsychic stress (mean difference -4.25, 95% confidence interval -6.83 to -1.67, P = 0.001, low-certainty evidence). It also seemed to have a positive effect on quality of life, although meta-analysis could not be conducted to pool the results. Virtual reality might reduce depression (standardised mean difference -0.48, 95% confidence interval -0.84 ~ -0.12, P = 0.009,very low- certainty evidence), but the evidence was uncertain, with similar results of anxiety, general level of stress, external stress, total cholesterol, and low-density lipoprotein. The evidence was uncertain about the effect of virtual reality on high-density lipoprotein (mean difference -1.79, 95% confidence interval -8.96 ~ 5.38, P = 0.62, very low-certainty evidence), with similar results of triglycerides and BMI.

Conclusions: Individuals undergoing cardiac rehabilitation may benefit from virtual reality since it can improve exercise capacity and psychological outcomes. More large, and well-designed studies with tailored virtual reality intervention are warranted to confirm the effects of virtual reality on individuals undergoing cardiac rehabilitation.

Tweetable Abstract: Virtual reality may benefit individuals undergoing cardiac rehabilitation since it can improve exercise capacity and psychological outcomes.
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http://dx.doi.org/10.1016/j.ijnurstu.2022.104323DOI Listing
July 2022

DNA hypermethylation of NOTCH2NLC in neuronal intranuclear inclusion disease: a case-control study.

J Neurol 2022 Jul 20. Epub 2022 Jul 20.

Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Background: GGC repeat expansions in NOTCH2NLC gene have been recently proposed to cause neuronal intranuclear inclusion disease (NIID) via prevailing gain-of-function mechanism (protein and RNA toxicity). Nevertheless, increasing evidences suggest that epigenetics can also play a role in the pathogenesis of repeat-mediated disorders.

Methods: In this study, using MethylTarget sequencing, we performed a quantitative analysis of the methylation status of 68 CpG sites located around the NOTCH2NLC promoter in 25 NIID patients and 25 age- and gender-matched healthy controls. We further explored the correlation of DNA methylation (DNAm) status with disease features and performed receiver operating characteristic (ROC) analysis.

Results: DNAm levels of GGC repeats and adjacent CpG islands were higher in the NIID patients than in controls, independent of gender and family history. DNAm levels at 4 CpG sites (CpG_207, CpG_421, GpG_473 and CpG_523) were negatively correlated with age at onset, and DNAm levels at 7 CpG sites (CpG_25, CpG_298, CpG_336, CpG_374, CpG_411, CpG_421 and CpG_473) were positively correlated with GGC repeats. NIID patients had concomitant system symptoms besides nervous system symptoms, and negative correlations between NOTCH2NLC DNAm levels and the number of multi-systemic involvement were observed in the study. The area under the ROC curve at NOTCH2NLC DNAm level reached to 0.733 for the best cutoff point of 0.012.

Conclusions: Our findings suggested the aberrant DNAm status of the NOTCH2NLC promoter in NIID, and we explored the link between DNAm levels and disease features quantitatively for the first time, which may help to further explore pathogenic mechanism.
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http://dx.doi.org/10.1007/s00415-022-11272-yDOI Listing
July 2022

[Research progress on pharmacology of butylphthalide and its derivatives].

Zhongguo Zhong Yao Za Zhi 2022 Jul;47(13):3425-3431

Institute of Medicinal Plant Development, Chinese Academy of Medicinal Sciences & Peking Union Medicinal College Beijing 100193, China Beijing Key Laboratory of Innovative Drug Discovery of Traditional Chinese Medicine(Natural Medicine) and Translational Medicine Beijing 100193, China.

The butylphthalide(NBP), a colorless or light yellow viscous oily component isolated from celery seeds, has the effects of anti-inflammation, anti-oxidative stress, protecting blood-brain barrier, improving cerebral microcirculation, and promoting angiogenesis. It can protect the neurological function of patients with ischemic stroke through a variety of mechanisms, improve the symptoms of patients, and contribute to the long-term recovery of them. Therefore, independently developed in China, NBP was approved by State Food and Drug Administration for the clinical treatment of stroke patients in 2002. At the same time, owing to the complex multi-target pharmacological mechanism, NBP has been frequently used in clinical practice. As frequently verified, it has obvious effects in the treatment of other neurological diseases such as Alzheimer's disease, vascular dementia, Parkinson's disease, autoimmune diseases, depression, traumatic central nervous system injury. Moreover, it demonstrates significant pharmacological effects on non-neurological diseases such as diabetes mellitus and myocardial infarction. Therefore, this study summarizes the research progress on roles of NBP in nervous system diseases and non-nervous system diseases, and the pharmacological characteristics and mechanisms of NBP, which is expected to lay a basis for research on related targets.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20220117.701DOI Listing
July 2022

Favoring Expression of Yak Alleles in Interspecies F1 Hybrids of Cattle and Yak Under High-Altitude Environments.

Front Vet Sci 2022 30;9:892663. Epub 2022 Jun 30.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.

Both - and -regulation could cause differential expression between the parental alleles in diploid species that might have broad biological implications. Due to the relatively distant genetic divergence between cattle and yak, as well as their differential adaptation to high-altitude environments, we investigated genome-wide allelic differential expression (ADE) in their F1 hybrids using Nanopore long-read RNA-seq technology. From adult F1 hybrids raised in high-altitude, ten lung and liver tissues were individually sequenced for producing 31.6 M full-length transcript sequences. Mapping against autosomal homologous regions between cattle and yak, we detected 17,744 and 14,542 protein-encoding genes expressed in lung and liver tissues, respectively. According to the parental assignments of transcript sequences, a total of 3,381 genes were detected to show ADE in at least one sample. There were 186 genes showing ubiquitous ADE in all the studied animals, and among them 135 and 37 genes had consistent higher expression of yak and cattle alleles, respectively. Functional analyses revealed that the genes with favoring expression of yak alleles have been involved in the biological progresses related with hypoxia adaptation and immune response. In contrast, the genes with favoring expression of cattle alleles have been enriched into different biological progresses, such as secretion of endocrine hormones and lipid metabolism. Our results would support unequal contribution of parental genes to environmental adaptation in the F1 hybrids of cattle and yak.
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http://dx.doi.org/10.3389/fvets.2022.892663DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280030PMC
June 2022

Autosomal dominant hypocalcemia with a novel mutation: a case study and literature review.

J Int Med Res 2022 Jul;50(7):3000605221110489

Department of Neurology, Suzhou Hospital of Anhui Medical University, Suzhou, China.

Autosomal dominant hypocalcemia type 1 (ADH1) is a rare inherited disorder characterized by hypocalcemia with low parathyroid hormone (PTH) levels and high urinary calcium. Its clinical presentation varies from mild asymptomatic to severe hypocalcemia. It is caused by gain-of-function mutations in the calcium-sensing receptor gene () which affect PTH secretion from the parathyroid gland and calcium resorption in the kidney. Here, we describe a case who presented with symptoms of recurrent seizure caused by hypocalcemia with a novel variant. We comprehensively analyzed the phenotypic features of this presentation and reviewed the current literature to better understand clinical manifestations and the genetic spectrum.
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http://dx.doi.org/10.1177/03000605221110489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9280832PMC
July 2022

Glycopattern Alteration of Glycoproteins in Gastrointestinal Cancer Cell Lines and Their Cell-Derived Exosomes.

J Proteome Res 2022 Aug 5;21(8):1876-1893. Epub 2022 Jul 5.

Institute of Genetics and Development Biology, Translational Medcine Institute, Xi'an Jiaotong University, Xi'an 710301, China.

Gastrointestinal (GI) cancers constitute the largest portion of all human cancers, and the most prevalent GI cancers in China are colorectal cancer (CRC), gastric cancer (GC), and hepatocellular carcinoma (HCC). Exosomes are nanosized vesicles containing proteins, lipids, glycans, and nucleic acid, which play important roles in the tumor microenvironment and progression. Aberrant glycosylation is closely associated with GI cancers; however, little is known about the glycopattern of the exosomes from GI cancer cells. In this study, glycopatterns of HCC, CRC, and GC cell lines and their exosomes were detected using lectin microarrays. For all exosomes, (GlcNAcβ1-4) and Galβ1-4GlcNAc (DSA) were the most abundant glycans, but αGalNAc and αGal (GSL-II and SBA) were the least. Different cancers had various characteristic glycans in either cells or exosomes. Glycans altered in cell-derived exosomes were not always consistent with the host cells in the same cancer. However, Fucα1-6GlcNAc (core fucose) and Fucα1-3(Galβ1-4)GlcNAc (AAL) were altered consistently in cells and exosomes although they were decreased in HCC and CRC but increased in GC. The study drew the full-scale glycan fingerprint of cells and exosomes related to GI cancer, which may provide useful information for finding specific biomarkers and exploring the underlying mechanism of glycosylation in exosomes.
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http://dx.doi.org/10.1021/acs.jproteome.2c00159DOI Listing
August 2022

Photo-tailored heterocrystalline covalent organic framework membranes for organics separation.

Nat Commun 2022 Jul 2;13(1):3826. Epub 2022 Jul 2.

Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.

Organics separation for purifying and recycling environment-detrimental solvents is essential to sustainable chemical industries. Covalent organic framework (COF) membranes hold great promise in affording precise and fast organics separation. Nonetheless, how to well coordinate facile processing-high crystalline structure-high separation performance remains a critical issue and a grand challenge. Herein, we propose a concept of heterocrystalline membrane which comprises high-crystalline regions and low-crystalline regions. The heterocrystalline COF membranes are fabricated by a two-step procedure, i.e., dark reaction for the construction of high-crystalline regions followed by photo reaction for the construction of low-crystalline regions, thus linking the high-crystalline regions tightly and flexibly, blocking the defect in high-crystalline regions. Accordingly, the COF membrane exhibits sharp molecular sieving properties with high organic solvent permeance up to 44-times higher than the state-of-the-art membranes.
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http://dx.doi.org/10.1038/s41467-022-31361-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250524PMC
July 2022

Effective single-stage revision using intra-articular antibiotic infusion after multiple failed surgery for periprosthetic joint infection : a mean seven years' follow-up.

Bone Joint J 2022 Jul;104-B(7):867-874

Department of Orthopaedics, First Affiliated Hospital of Xinjiang Medical University, Ürümqi, China.

Aims: Periprosthetic joint infections (PJIs) with prior multiple failed surgery for reinfection represent a huge challenge for surgeons because of poor vascular supply and biofilm formation. This study aims to determine the results of single-stage revision using intra-articular antibiotic infusion in treating this condition.

Methods: A retrospective analysis included 78 PJI patients (29 hips; 49 knees) who had undergone multiple prior surgical interventions. Our cohort was treated with single-stage revision using a supplementary intra-articular antibiotic infusion. Of these 78 patients, 59 had undergone more than two prior failed debridement and implant retentions, 12 patients had a failed arthroplasty resection, three hips had previously undergone failed two-stage revision, and four had a failed one-stage revision before their single-stage revision. Previous failure was defined as infection recurrence requiring surgical intervention. Besides intravenous pathogen-sensitive agents, an intra-articular infusion of vancomycin, imipenem, or voriconazole was performed postoperatively. The antibiotic solution was soaked into the joint for 24 hours for a mean of 16 days (12 to 21), then extracted before next injection. Recurrence of infection and clinical outcomes were evaluated.

Results: A total of 68 patients (87.1%) were free of infection at a mean follow-up time of 85 months (24 to 133). The seven-year infection-free survival was 87.6% (95% confidence interval (CI) 79.4 to 95.8). No significant difference in infection-free survival was observed between hip and knee PJIs (91.5% (95% CI 79.9 to 100) vs 84.7% (95% CI 73.1 to 96.3); p = 0.648). The mean postoperative Harris Hip Score was 76.1 points (63.2 to 92.4) and Hospital for Special Surgery score was 78. 2 (63.2 to 92.4) at the most recent assessment. Polymicrobial and fungal infections accounted for 14.1% (11/78) and 9.0% (7/78) of all cases, respectively.

Conclusion: Single-stage revision with intra-articular antibiotic infusion can provide high antibiotic concentration in synovial fluid, thereby overcoming reduced vascular supply and biofilm formation. This supplementary route of administration may be a viable option in treating PJI after multiple failed prior surgeries for reinfection. Cite this article:  2022;104-B(7):867-874.
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http://dx.doi.org/10.1302/0301-620X.104B7.BJJ-2021-1704.R1DOI Listing
July 2022

Charged Nanochannels in Covalent Organic Framework Membranes Enabling Efficient Ion Exclusion.

ACS Nano 2022 Jun 30. Epub 2022 Jun 30.

Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.

Controllable ion transport through nanochannels is crucial for biological and artificial membrane systems. Covalent organic frameworks (COFs) with regular and tunable nanochannels are emerging as an ideal material platform to develop synthetic membranes for ion transport. However, ion exclusion by COF membranes remains challenging because most COF materials have large-sized nanochannels leading to nonselective transport of small ions. Here we develop ionic COF membranes (iCOFMs) to control ion transport through charged framework nanochannels, the interior surfaces of which are covered with arrayed sulfonate groups to render superior charge density. The overlap of an electrical double layer in charged nanochannels blocks the entry of co-ions, narrows their passageways, and concomitantly restrains the permeation of counterions the charge balance. These highly charged large-sized nanochannels within the iCOFM enable ion exclusion while maintaining intrinsically high water permeability. Our results reveal possibilities for controllable ion transport based on COF membranes for water purification, ionic separation, sensing, and energy conversion.
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http://dx.doi.org/10.1021/acsnano.2c04767DOI Listing
June 2022

RNA-Seq analysis identifies alterations of the primary cilia gene SPAG17 and SOX9 locus non-coding RNAs in systemic sclerosis.

Arthritis Rheumatol 2022 Jun 28. Epub 2022 Jun 28.

Feinberg School of Medicine, Scleroderma Program, Northwestern University, Chicago, IL, USA.

Objective: Systemic sclerosis (SSc) is characterized by immune activation, vasculopathy, and unresolving fibrosis in the skin, lungs, and other organs. We performed RNA-Seq analysis on skin biopsies and peripheral blood mononuclear cells (PBMCs) from SSc patients and controls to better understand SSc pathogenesis.

Methods: We analyzed these data to 1) test for case-control differences, and 2) identify genes whose expression levels correlate with SSc severity as measured by local skin score, modified Rodnan skin score (MRSS), forced vital capacity (FVC), or diffusion capacity for carbon monoxide (DLCO).

Results: We found that PBMCs from SSc patients showed a strong type 1 interferon signature. This signal replicated in the skin, with additional signals for increased extracellular matrix (ECM) genes, classical complement pathway activation, and the presence of B cells. Notably, we observed a marked decrease in the expression of SPAG17, a cilia component, in SSc skin. We identified genes that correlated with MRSS, DLCO, and FVC in SSc PBMCs and skin using weighted gene co-expression analysis (WGCNA). These genes were largely distinct from the case/control differentially expressed genes. In PBMCs, type 1 interferon signatures negatively correlated with DLCO. In SSc skin, ECM gene expression positively correlated with MRSS. Network analysis of SSc skin genes correlated with clinical features identified the non-coding RNAs SOX9-AS1 and ROCR, both near the SOX9 locus, as highly connected, "hub-like" genes in the network.

Conclusion: These results identify non-coding RNAs and SPAG17 as novel factors potentially implicated in SSc pathogenesis.
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http://dx.doi.org/10.1002/art.42281DOI Listing
June 2022

Giant Osmotic Energy Conversion through Vertical-Aligned Ion-Permselective Nanochannels in Covalent Organic Framework Membranes.

J Am Chem Soc 2022 Jul 28;144(27):12400-12409. Epub 2022 Jun 28.

Division of Physical Science and Engineering, 4700 King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia.

Nanofluidic membranes have been demonstrated as promising candidates for osmotic energy harvesting. However, it remains a long-standing challenge to fabricate high-efficiency ion-permselective membranes with well-defined channel architectures. Here, we demonstrate high-performance osmotic energy conversion membranes based on oriented two-dimensional covalent organic frameworks (COFs) with ultrashort vertically aligned nanofluidic channels that enabled efficient and selective ion transport. Experiments combined with molecular dynamics simulations revealed that exquisite control over channel orientation, charge polarity, and charge density contributed to high ion selectivity and permeability. When applied to osmotic energy conversion, a pair of 100 nm thick oppositely charged COF membranes achieved an ultrahigh output power density of 43.2 W m at a 50-fold salinity gradient and up to 228.9 W m for the Dead Sea and river water system. The achieved power density outperforms the state-of-the-art nanofluidic membranes, suggesting the great potential of oriented COF membranes in the fields of advanced membrane technology and energy conversion.
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http://dx.doi.org/10.1021/jacs.2c04223DOI Listing
July 2022

Microglia Regulate Blood-Brain Barrier Integrity via MiR-126a-5p/MMP9 Axis during Inflammatory Demyelination.

Adv Sci (Weinh) 2022 Jun 27:e2105442. Epub 2022 Jun 27.

Institute of Neuroscience, Key Laboratory of Molecular Neurobiology of Ministry of Education and the Collaborative Innovation Center for Brain Science, SMMU, Shanghai, 200433, China.

Blood-brain barrier (BBB) impairment is an early prevalent feature of multiple sclerosis (MS), and remains vital for MS progression. Microglial activation precedes BBB disruption and cellular infiltrates in the brain of MS patients. However, little is known about the function of microglia in BBB impairment. Here, microglia acts as an important modulator of BBB integrity in inflammatory demyelination. Microglial depletion profoundly ameliorates BBB impairment in experimental autoimmune encephalomyelitis (EAE). Specifically, miR-126a-5p in microglia is positively correlated with BBB integrity in four types of MS plaques. Mechanistically, microglial deletion of miR-126a-5p exacerbates BBB leakage and EAE severity. The protective effect of miR-126a-5p is mimicked and restored by specific inhibition of MMP9 in microglia. Importantly, Auranofin, an FDA-approved drug, is identified to protect BBB integrity and mitigate EAE progression via a microglial miR-126a-5p dependent mechanism. Taken together, microglia can be manipulated to protect BBB integrity and ameliorate inflammatory demyelination. Targeting microglia to regulate BBB permeability merits consideration in therapeutic interventions in MS.
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http://dx.doi.org/10.1002/advs.202105442DOI Listing
June 2022

Analysis and Prospect of the Application of Wireless Sensor Networks in Ubiquitous Power Internet of Things.

Comput Intell Neurosci 2022 15;2022:9004942. Epub 2022 Jun 15.

School of Intelligent Manufacturing and Electronic Engineering, Wenzhou University of Technology, Wenzhou 325035, China.

With the rapid development of the economy and society, the low efficiency and high loss of the traditional power grid can no longer meet the growing social demand, and the power grid market is facing a reform. Smart grid, as a next-generation power system, it can effectively improve the performance of traditional power grids. The ubiquitous power Internet of Things (UPIOT) replaces the traditional grids with efficient, safe, reliable, and flexible new grids, improves the utilization efficiency of the grid, reduces the loss of the power grid in the transmission process, and can meet the needs of different types of markets and users. As an advanced information acquisition and processing technology, wireless sensor networks have been widely used in medical, industrial, agricultural, commercial, and public management fields. It is an important means to promote future economic development and build a harmonious society. In the power system, wireless sensor network technology can be widely used in many fields such as line fault location, real-time monitoring, smart meter reading, and relay protection. In this paper, the basic concepts and overall architecture of ubiquitous power Internet of Things are summarized. Then, we summarize the research status of the wireless sensor network in smart grid, including power equipment, line monitoring, smart grid wireless automatic meter reading, distribution network relay protection, power assets life-cycle management, power grid fault location, and power grid fault diagnosis. In view of the technical characteristics of wireless sensor networks, combined with the production links of power systems, the application framework of wireless sensor network technology in the power systems is constructed. The application of wireless sensor networks is prospected from the aspects of network development of relay protection, application research of smart substation, application research of power grid catastrophe, security protection of power system, and deep-seated ubiquitous power Internet of Things.
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http://dx.doi.org/10.1155/2022/9004942DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9217569PMC
June 2022

The Significance of Neutrophil Extracellular Traps in Colorectal Cancer and Beyond: From Bench to Bedside.

Front Oncol 2022 7;12:848594. Epub 2022 Jun 7.

Department of General Surgery, the First Medical Centre, Chinese PLA General Hospital, Beijing, China.

Neutrophil extracellular traps (NETs), products of neutrophil death when exposed to certain stimuli, were first proposed as a type of response to bacterial infection in infectious diseases. Since then, extensive studies have discovered its involvement in other non-infectious inflammatory diseases including thromboembolism, autoimmune diseases, and cancer. Colorectal cancer (CRC) is one of the most common malignancies in the world. NET formation is closely associated with tumorigenesis, progression, and metastasis in CRC. Therefore, the application of NETs in clinical practice as diagnostic biomarkers, therapeutic targets, and prognostic predictors has a promising prospect. In addition, therapeutics targeting NETs are significantly efficient in halting tumor progression in preclinical cancer models, which further indicates its potential clinical utility in cancer treatment. This review focuses on the stimuli of NETosis, its pro-tumorigenic activity, and prospective clinical utility primarily in but not limited to CRC.
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http://dx.doi.org/10.3389/fonc.2022.848594DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209713PMC
June 2022

Exploration of the Common Gene Characteristics and Molecular Mechanism of Parkinson's Disease and Crohn's Disease from Transcriptome Data.

Brain Sci 2022 Jun 13;12(6). Epub 2022 Jun 13.

School of Medicine, Anhui University of Science and Technology, Huainan 232001, China.

Parkinson's disease (PD) is the second most common neurodegenerative disorder, and the mechanism of its occurrence is still not fully elucidated. Accumulating evidence has suggested that the gut acts as a potential origin of PD pathogenesis. Recent studies have identified that inflammatory bowel disease acts as a risk factor for Parkinson's disease, although the underlying mechanisms remain elusive. The aim of this study was to further explore the molecular mechanism between PD and Crohn's disease (CD). The gene expression profiles of PD (GSE6613) and CD (GSE119600) were downloaded from the Gene Expression Omnibus (GEO) database and were identified as the common differentially expressed genes (DEGs) between the two diseases. Next, analyses were performed, including functional enrichment analysis, a protein-protein interaction network, core genes identification, and clinical correlation analysis. As a result, 178 common DEGs (113 upregulated genes and 65 downregulated genes) were found between PD and CD. The functional analysis found that they were enriched in regulated exocytosis, immune response, and lipid binding. Twelve essential hub genes including BUB1B, BUB3, DLGAP5, AURKC, CBL, PCNA, RAF1, LYN, RPL39L, MRPL13, RSL24D1, and MRPS11 were identified from the PPI network by using cytoHubba. In addition, inflammatory and metabolic pathways were jointly involved in these two diseases. After verifying expression levels in an independent dataset (GSE99039), a correlation analysis with clinical features showed that LYN and RAF1 genes were associated with the severity of PD. In conclusion, our study revealed the common pathogenesis of PD and CD. These common pathways and hub genes may provide novel insights for mechanism research.
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http://dx.doi.org/10.3390/brainsci12060774DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9221146PMC
June 2022

Conjugated microporous polymer membranes for light-gated ion transport.

Sci Adv 2022 Jun 17;8(24):eabo2929. Epub 2022 Jun 17.

Chemical Engineering Program, Division of Physical Science and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.

Inspired by the light-gated ion channels in cell membranes that play important roles in many biological activities, herein, we developed an artificial light-gated ion channel membrane out of conjugated microporous polymers. Through bottom-up design of the monomer molecular structure and by the electropolymerization method, the membrane pore size and thickness were precisely controlled on the molecular level. The obtained membrane exhibited uniform pore size and highly sensitive light-switchable response. The photoisomerization of the polymer chain resulted in a reversible "on and off" light control over the pore size and subsequently led to light-gated ion transport across the membrane for a series of ions including hydrogen, potassium, sodium, lithium, calcium, magnesium, and aluminum ions.
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http://dx.doi.org/10.1126/sciadv.abo2929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205585PMC
June 2022

New phenotype of RTN2-related spectrum: Complicated form of spastic paraplegia-12.

Ann Clin Transl Neurol 2022 Jun 9. Epub 2022 Jun 9.

Department of Neurology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.

Objective: Spastic paraplegia-12 (SPG12) is a subtype of hereditary spastic paraplegia caused by Reticulon-2 (RTN2) mutations. We described the clinical and genetic features of three SPG12 patients, functionally explored the potential pathogenic mechanism of RTN2 mutations, and reviewed RTN2-related cases worldwide.

Methods: The three patients were 31, 36, and 50 years old, respectively, with chronic progressive lower limb spasticity and walking difficulty. Physical examination showed elevated muscle tone, hyperreflexia and Babinski signs in the lower limbs. Patients 1 and 3 additionally had visual, urinary, and/or coordination dysfunctions. Patient 2 also had epileptic seizures. RTN2 mutations were identified by whole-exome sequencing, followed by Sanger sequencing, segregation analysis, and phenotypic reevaluation. Functional examination of identified mutations was further explored.

Results: Three variants in RTN2 were identified in Patient 1 (c.103C>T, p.R35X), Patient 2 (c.230G>A, p.G77D), and Patient 3 (c.337C>A, p.P113T) with SPG, respectively. Western blotting revealed the p.R35X with smaller molecular weight than WT and other two missense mutants. Immunostaining showed the wild type colocalized with endoplasmic reticulum (ER) in vitro. p.R35X mutant diffusely distributes in the cytoplasm, losing colocalization with ER. p.G77D and p.P113T co-localized with ER, which was abnormally aggregated in clumps.

Interpretation: In this study, we identified three cases with complicated SPG12 due to three novel RTN2 mutations, respectively, presenting various phenotypes: classic SPG symptoms with (1) visual abnormalities and sphincter disturbances or (2) seizures. The phenotypic heterogeneity might arise from the abnormal subcellular localization of mutant Reticulon-2 and improper ER morphogenesis, revealing the RTN2-related spectrum is still expanding.
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http://dx.doi.org/10.1002/acn3.51605DOI Listing
June 2022

The Association between mHealth App Use and Healthcare Satisfaction among Clients at Outpatient Clinics: A Cross-Sectional Study in Inner Mongolia, China.

Int J Environ Res Public Health 2022 06 5;19(11). Epub 2022 Jun 5.

Department of Epidemiology, Faculty of Medicine, Prince of Songkla University, Hat Yai 90110, Thailand.

Mobile health (mHealth) applications (apps) have been developed in hospital settings to allocate and manage medical care services, which is one of the national strategies to improve health care in China. Little is known about the comprehensive effects of hospital-based mHealth app use on client satisfaction. The aim of this study was to determine the relationship between the full range of mHealth app use and satisfaction domains among clients attending outpatient clinics. A cross-sectional survey was conducted from January to February 2021 in twelve tertiary hospitals in Inner Mongolia. After the construction of the mHealth app use, structural equation modeling was used for data analysis. Of 1889 participants, the standardized coefficients β on environment/convenience, health information, and medical service fees were 0.11 ( < 0.001), 0.06 ( = 0.039), and 0.08 ( = 0.004), respectively. However, app use was not significantly associated with satisfaction of doctor-patient communication (β = 0.05, = 0.069), short-term outcomes (β = 0.05, = 0.054), and general satisfaction (β = 0.02, = 0.429). Clients of the study hospitals were satisfied with the services, but their satisfaction was not much associated with mHealth use. The limitation of the mHealth system should be improved to enhance communication and engagement among clients, doctors, and healthcare givers, as well as to pay more attention to health outcomes and satisfaction of clients.
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http://dx.doi.org/10.3390/ijerph19116916DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180655PMC
June 2022

Cornus officinalis vinegar reduces body weight and attenuates hepatic steatosis in mouse model of nonalcoholic fatty liver disease.

J Food Sci 2022 Jul 8;87(7):3248-3259. Epub 2022 Jun 8.

College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, Henan, P.R. China.

This study aimed to determine the main bioactive components of Cornus officinalis vinegar (COV) and assess the effects of COV on the body weight (BW) and hepatic steatosis in a nonalcoholic fatty liver disease (NAFLD) mouse model. Seven-week-old KM female mice were divided into five treatment groups: (1) Normal control (NC) group, (2) high fat diet (HFD) group, (3) low concentration treatment group (3.5% COV), (4) medium concentration treatment group (5.0% COV), and (5) high concentration treatment group (6.5% COV). Mice in the NC group were fed with a normal chow diet, and those in the other four groups were fed with a HFD known for causing obesity for 10 weeks. Then, mice in the three COV treatment groups were orally administered with COV once a day for 6 weeks. Results showed that the contents of loganin and morroniside in COV reached 16.82 and 51.17 µg/ml, respectively, and COV also contained multiple organic acids. COV significantly reduced BW, abdominal fat weight, liver weight, and the levels of glucose, triglyceride, and low-density lipoprotein cholesterol of serum and increased the levels of high-density lipoprotein cholesterol of serum (p < 0.05). COV also improved the liver function and anti-oxidant activity of liver (p < 0.05). COV treatments increased the interleukin-10 expression and reduced the tumor necrosis factor-α expression in the liver tissue of NAFLD mice (p < 0.05). Histopathological observation revealed that COV suppressed hepatic lipid accumulation and steatosis. The results suggest that COV may contribute to the alleviation of NAFLD and obesity.
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http://dx.doi.org/10.1111/1750-3841.16178DOI Listing
July 2022

Assembling covalent organic framework membranes via phase switching for ultrafast molecular transport.

Nat Commun 2022 Jun 7;13(1):3169. Epub 2022 Jun 7.

Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, China.

Fabrication of covalent organic framework (COF) membranes for molecular transport has excited highly pragmatic interest as a low energy and cost-effective route for molecular separations. However, currently, most COF membranes are assembled via a one-step procedure in liquid phase(s) by concurrent polymerization and crystallization, which are often accompanied by a loosely packed and less ordered structure. Herein, we propose a two-step procedure via a phase switching strategy, which decouples the polymerization process and the crystallization process to assemble compact and highly crystalline COF membranes. In the pre-assembly step, the mixed monomer solution is casted into a pristine membrane in the liquid phase, along with the completion of polymerization process. In the assembly step, the pristine membrane is transformed into a COF membrane in the vapour phase of solvent and catalyst, along with the completion of crystallization process. Owing to the compact and highly crystalline structure, the resultant COF membranes exhibit an unprecedented permeance (water ≈ 403 L m bar h and acetonitrile ≈ 519 L m bar h). Our two-step procedure via phase switching strategy can open up a new avenue to the fabrication of advanced organic crystalline microporous membranes.
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http://dx.doi.org/10.1038/s41467-022-30647-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174484PMC
June 2022

Financial toxicity and psychological distress in adults with cancer: A treatment-based analysis.

Asia Pac J Oncol Nurs 2022 Sep 20;9(9):100069. Epub 2022 Apr 20.

Liaoning Office for Cancer Control and Research, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang, China.

Objective: To evaluate disparities in financial toxicity and psychological distress in patients with cancer as a function of treatment and reveal the relationship between financial toxicity and psychological distress.

Methods: This was a multicenter cross-sectional study. Patients were recruited from March 2017 to October 2018, and questionnaires were completed regarding their demographics, financial toxicity, and psychological distress. A multiple linear regression model was used to examine factors associated with financial and psychological distress.

Results: Significant financial toxicity and psychological distress occurred in 47.9% and 56.5% of patients, respectively. Financial toxicity ( ​= ​0.032) and psychological distress ( ​< ​0.001) were statistically different among the single chemotherapy, adjuvant therapy, and surgery groups. Multivariable analysis revealed that patients aged 50-59 years ( ​= ​0.035), 60-69 years ( ​= ​0.007), and 70 years or older ( ​= ​0.002) had higher the Comprehensive Score for financial Toxicity (COST) scores compared with patients less than 50 years old. Patients with personal annual income > 40,000 CNY reported higher COST scores than those who had < 20,000 CNY ( ​< ​0.001). Patients who had Urban Resident Basic Medical Insurance (URBMI) ( ​= ​0.030) or New Rural Cooperative Medical Scheme (NRCMS) ( ​= ​0.006) compared with Urban Employee Basic Medical Insurance (UEBMI) presented lower COST scores than patients with UEBMI. The multiple analysis model of psychological distress showed that an age of more than 70 years ( ​= ​0.010) was significantly associated with low the Distress Thermometer (DT) scores, and patients with colorectal cancer ( ​= ​0.009), the surgery group ( ​< ​0.001) and adjuvant therapy group ( ​< ​0.001) were significantly associated with high DT scores. The correlations between financial toxicity and psychological distress were mild but statistically significant in the chemotherapy-related treatment groups.

Conclusions: The research highlights the high rates of financial and psychological distress in adult patients. Multidimensional distress screening and psychosocial interventions should be provided for patients with cancer according to related factors.
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http://dx.doi.org/10.1016/j.apjon.2022.04.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9166374PMC
September 2022

Src-homology domain 2 containing protein tyrosine phosphatase-1 (SHP-1) directly binds to proto-oncogene tyrosine-protein kinase Src (c-Src) and promotes the transcriptional activation of connexin 43 (Cx43).

Bioengineered 2022 05;13(5):13534-13543

Department of Critical Care Medicine, University-Town Hospital of Chongqing Medical University, Chongqing, China.

The prevalence of atrial fibrillation (AF), which is one of the common arrhythmias in clinics, is increasing sharply and has affected millions of patients, which is expected to triple by 2050. The purpose of the study was to explore the regulatory relationship between Src-homology domain 2 containing protein tyrosine phosphatase-1 (SHP-1) and proto-oncogene tyrosine-protein kinase Src (c-Src) and the regulation of Connexins 43 (Cx43), and its effect on AF was also studied. Mouse atrial myocyte line (HL-1 cell line) was used as the research object. After overexpression of SHP-1, the expressions of p-c-Src, Cx43, and SHP-1 were detected by Western blot and cellular immunofluorescence, respectively. The location and interaction of SHP-1 and c-Src in the cells were detected by immunofluorescence co-localization and co-immunoprecipitation (Co-IP). The regulation of c-Src and Cx43 was detected by DNA pull down, chromatin co-immunoprecipitation (CHIP), and dual-luciferase reporter system. The results revealed that overexpression of SHP-1 could inhibit the phosphorylation and activation of c-Src and increase the expression of Cx43. Moreover, there was a direct binding between SHP-1 and c-Src, and c-Src could bind to the promoter region of Cx43 and inhibit the transcription of Cx43. In conclusion, SHP-1 could bind to c-Src and inhibit the activity of c-Src, thus enhancing the transcriptional activation of Cx43 and improving the function of gap junction.
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http://dx.doi.org/10.1080/21655979.2022.2079252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276044PMC
May 2022

Encoding latent SuFEx reactive meta-fluorosulfate tyrosine to expand covalent bonding of proteins.

Chem Commun (Camb) 2022 Jun 14;58(48):6861-6864. Epub 2022 Jun 14.

Department of Pharmaceutical Chemistry and the Cardiovascular Research Institute, University of California San Francisco, 555 Mission Bay Boulevard South, San Francisco, California 94158, USA.

The introduction of new covalent bonds into proteins is affording novel avenues for protein research and applications, yet it remains difficult to generate covalent linkages at all possible sites and across diverse protein classes. Herein, we genetically encoded -fluorosulfate-L-tyrosine (mFSY) to selectively react with lysine, tyrosine, and histidine proximity-enabled SuFEx reaction. mFSY was able to target residues that were elusive for previous unnatural amino acids, and permitted engineering of various proteins including affibody, nanobody, and Fab into covalent binders that irreversibly cross-linked EGFR and HER2. mFSY is thus valuable for developing covalent proteins for biological research, synthetic biology, and biotherapeutics.
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http://dx.doi.org/10.1039/d2cc01902gDOI Listing
June 2022

The Sociodemographic Digital Divide in Mobile Health App Use Among Clients at Outpatient Departments in Inner Mongolia, China: Cross-sectional Survey Study.

JMIR Hum Factors 2022 May 19;9(2):e36962. Epub 2022 May 19.

Department of Epidemiology, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.

Background: Mobile health (mHealth) apps have become part of the infrastructure for access to health care in hospitals, especially during the COVID-19 pandemic. However, little is known about the effects of sociodemographic characteristics on the digital divide regarding the use of hospital-based mHealth apps and their benefits to patients and caregivers.

Objective: The aim of this study was to document the cascade of potential influences from digital access to digital use and then to mHealth use, as well as the potential influence of sociodemographic variables on elements of the cascade.

Methods: A cross-sectional survey was conducted from January to February 2021 among adult clients at outpatient departments in 12 tertiary hospitals of Inner Mongolia, China. Structural equation modeling was conducted after the construct comprising digital access, digital use, and mHealth use was validated.

Results: Of 2115 participants, the β coefficients (95% CI) of potential influence of digital access on digital use, and potential influence of digital use on mHealth use, were 0.28 (95% CI 0.22-0.34) and 0.51 (95% CI 0.38-0.64), respectively. Older adults were disadvantaged with regard to mHealth access and use (β=-0.38 and β=-0.41), as were less educated subgroups (β=-0.24 and β=-0.27), and these two factors had nonsignificant direct effects on mHealth use.

Conclusions: To overcome the mHealth use divide, it is important to improve digital access and digital use among older adults and less educated groups.
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http://dx.doi.org/10.2196/36962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9164102PMC
May 2022

A TetR family transcriptional regulator, SP_2854 can affect the butenyl-spinosyn biosynthesis by regulating glucose metabolism in Saccharopolyspora pogona.

Microb Cell Fact 2022 May 14;21(1):83. Epub 2022 May 14.

Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, China.

Background: Butenyl-spinosyn produced by Saccharopolyspora pogona exhibits strong insecticidal activity and a broad pesticidal spectrum. Currently, important functional genes involve in butenyl-spinosyn biosynthesis remain unknown, which leads to difficulty in efficiently understanding its regulatory mechanism, and improving its production by metabolic engineering.

Results: Here, we identified a TetR family transcriptional regulator, SP_2854, that can positively regulate butenyl-spinosyn biosynthesis and affect strain growth, glucose consumption, and mycelial morphology in S. pogona. Using targeted metabolomic analyses, we found that SP_2854 overexpression enhanced glucose metabolism, while SP_2854 deletion had the opposite effect. To decipher the overproduction mechanism in detail, comparative proteomic analysis was carried out in the SP-2854 overexpressing mutant and the original strain, and we found that SP_2854 overexpression promoted the expression of proteins involved in glucose metabolism.

Conclusion: Our findings suggest that SP_2854 can affect strain growth and development and butenyl-spinosyn biosynthesis in S. pogona by controlling glucose metabolism. The strategy reported here will be valuable in paving the way for genetic engineering of regulatory elements in actinomycetes to improve important natural products production.
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http://dx.doi.org/10.1186/s12934-022-01808-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107242PMC
May 2022
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