Publications by authors named "Leonardo Rescio"

3 Publications

  • Page 1 of 1

Enzyme-aided extraction of lycopene from high-pigment tomato cultivars by supercritical carbon dioxide.

Food Chem 2015 Mar 24;170:193-202. Epub 2014 Aug 24.

Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali (Di.S.Te.B.A.), Università del Salento, via Prov.le Lecce-Monteroni, 73100 Lecce, Italy. Electronic address:

This work reports a novel enzyme-assisted process for lycopene concentration into a freeze-dried tomato matrix and describes the results of laboratory scale lycopene supercritical CO2 (SC-CO2) extractions carried out with untreated (control) and enzyme-digested matrices. The combined use of food-grade commercial plant cell-wall glycosidases (Celluclast/Novozyme plus Viscozyme) allows to increase lycopene (∼153%) and lipid (∼137%) concentration in the matrix and rises substrate load onto the extraction vessel (∼46%) compared to the control. The addition of an oleaginous co-matrix (hazelnut seeds) to the tomato matrix (1:1 by weight) increases CO2 diffusion through the highly dense enzyme-treated matrix bed and provides lipids that are co-extracted increasing lycopene yield. Under the same operative conditions (50 MPa, 86 °C, 4 mL min(-1) SC-CO2 flow) extraction yield from control and Celluclast/Novozyme+Viscozyme-treated tomato matrix/co-matrix mixtures was similar, exceeding 75% after 4.5h of extraction. However, the total extracted lycopene was ∼3 times higher in enzyme-treated matrix than control.
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March 2015

Optimisation of biological and physical parameters for lycopene supercritical CO2 extraction from ordinary and high-pigment tomato cultivars.

J Sci Food Agric 2010 Aug;90(10):1709-18

Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Prov. le Lecce-Monteroni, Lecce, Italy.

Background: Lycopene is used for several industrial applications. Supercritical CO(2) (SC-CO(2)) extraction from red-ripe tomato fruits is an excellent technique to replace the use of harmful solvents. In this study, starting from red-ripe tomatoes of ordinary and high-lycopene cultivars, the effect of different agronomical and technical aspects on lycopene content, stability and yield was evaluated throughout the production process from fresh tomatoes to the final SC-CO(2)-extracted oleoresin containing lycopene.

Results: Red-ripe tomato cultivars differed in their lycopene content. Irrigation excess or deficit caused an increase in the amount of lycopene in the fruits. Fresh tomatoes were processed into a lyophilised matrix suitable for SC-CO(2) extraction, which could be stored for more than 6 months at -20 degrees C without lycopene loss. Under the optimal extraction conditions, efficiencies of up to 80% were achieved, but the recovery of lycopene in the extracted oleoresin was very low (approximately 24%). Co-extraction of the tomato matrix mixed with a lipid co-matrix allowed the recovery of approximately 90% of lycopene in the oleoresin. Using the high-lycopene cultivars, the yield of total extracted lycopene increased by approximately 60% with respect to the ordinary cultivars. Lipids and other biologically active molecules were present in the oleoresin.

Conclusion: A method for extracting, from a tomato matrix, a natural and solvent-free oleoresin containing lycopene dissolved in a highly unsaturated vegetable oil has been described. The oleoresin represents an excellent product for testing on cancer and cardiovascular disease prevention.
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August 2010

Identification of anthocyanins in Rhamnus alaternus L. berries.

J Agric Food Chem 2005 Mar;53(5):1723-7

Dipartimento di Ingegneria dell'Innovazione, Università di Lecce, via per Arnesano km 1, 73100 Lecce, Italy.

Anthocyanin composition in berries of Rhamnus alaternus L., a perennial wild shrub typical of the Mediterranean area, was determined for the first time. The pigments were extracted from the berries with 0.1% HCl in methanol and purified using a C-18 solid-phase cartridge. High-performance liquid chromatography diode array detection-mass spectrometry analysis showed that delphinidin 3-O-rutinoside represented about 62.4% of the total pigments. Other anthocyanins were 3-O-rutinoside derivatives of cyanidin (8.4%), petunidin (15.8%), pelargonidin (4.7%), and peonidin and malvidin (8.7%). The concomitant presence of the six most common anthocyanidins suggested that R. alaternus berries, besides being a good pigment source, could also be a useful tool for anthocyanin identification.
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March 2005