Publications by authors named "Lenka Vargová"

8 Publications

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A very rare case of possible actinomycosis of the mandible from the Middle Ages.

Int J Paleopathol 2020 12 16;31:53-59. Epub 2020 Sep 16.

Laboratory of Biological and Molecular Anthropology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic. Electronic address:

Objective: Documented cases of actinomycosis in archaeological skeletons are very rare, especially from Central Europe. Our contribution will help facilitate the differential diagnosis of this disease for other paleopathologists.

Material: This paper describes a pathological finding of the skeleton of a 40-year-old male from a burial ground in Sady-Špitálky (Czech Republic) dated to the 10th-12th century.

Methods: The affected skeleton was evaluated as a probable case of actinomycosis on the basis of a detailed macroscopic, X-ray and histological examination. The osteolytic foci examined were compared with similar changes caused by tuberculosis, syphilis and mycoses.

Results: The character and location of the defect on the mandible is indicative of organ actinomycosis and is also reflected by the lytic lesion observed on a lumbar vertebra.

Conclusions: The described case can be considered one of the very rare paleopathological findings of possible actinomycosis in humans in Central Europe.

Significance: Good evidence of bone actinomycosis findings may be beneficial for further paleopathological and epidemiological studies, especially for research focused on the diachronic development of actinomycosis in Europe. In doing so, all available factors, such as hygiene habits, nutrition, social structure and overall health of the population that could be causally related to its origin, course and treatment, can be taken into account.

Limitations: The mandible of the studied individual was damaged, especially in the area affected by the lesion, so the paleopathological analysis was difficult to perform.

Suggestion For The Future Research: In future, actinomycosis in this skeleton may be confirmed by bio-molecular analysis.
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http://dx.doi.org/10.1016/j.ijpp.2020.09.001DOI Listing
December 2020

A brief history of tuberculosis in the Czech Lands.

Tuberculosis (Edinb) 2017 07 19;105:35-48. Epub 2017 Apr 19.

Division of Medical Anthropology, Department of Anatomy, Faculty of Medicine, Masaryk University, Kamenice 3, 625 00 Brno, Czech Republic. Electronic address:

Tuberculosis currently remains a serious medical problem, therefore increased attention is being paid to this disease. Paleopathological studies focused on the monitoring of morbid changes in skeletal remains of historical populations facilitate a detailed study of the development of this disease. They provide direct evidence of the existence of tuberculosis and its past forms. In addition to literary and iconographic sources, the present study is focused on recording the findings of bone tuberculosis in historical osteological sets from the Czech Lands and is the starting point for their detailed review. Approximately 76 cases of bone tuberculosis from the Czech Lands have been published and more or less reliably documented from 20 archeological sites dated back from the Eneolithic to the modern period.
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http://dx.doi.org/10.1016/j.tube.2017.04.006DOI Listing
July 2017

F-FDG PET/CT in polymyalgia rheumatica-a pictorial review.

Br J Radiol 2017 Aug 16;90(1076):20170198. Epub 2017 Jun 16.

8 Rheumatology Unit, 2nd Department of Internal Medicine, St Anne's University Hospital Brno and Masaryk University, Brno, Czech Republic.

Polymyalgia rheumatica (PMR) is one of the inflammatory rheumatic diseases that can potentially be detected by positron emission tomography/CT. High fluorine-18 fludeoxyglucose (F-FDG) accumulation around the shoulders, sternoclavicular and hip joints are the most common pre-treatment features of patients with PMR. Another common sign is increased F-FDG uptake in extra-articular regions between columnal spinous processes, near ischial tuberosities and in the praepubic area. Some patients also present with high F-FDG uptake in main arteries, corresponding to the characteristics of giant cell arteritis. It is possible to observe a decrease or even a disappearance of F-FDG uptake after effective therapy, an event which may be useful for the monitoring of treatment as well as for detection of PMR relapse.
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http://dx.doi.org/10.1259/bjr.20170198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603955PMC
August 2017

The possibilities of studying human embryos and foetuses using micro-CT: a technical note.

Anat Sci Int 2017 Mar 11;92(2):299-303. Epub 2016 Oct 11.

CEITEC BUT-Central European Institute of Technology, Brno University of Technology, Technická 3058/10, 616 00, Brno, Czech Republic.

The purpose of the study was to discover a way to study the internal structure and evolution of human embryos noninvasively. The human embryo was stained with phosphotungstic acid solution (PTA) in ethanol (EPTA) and scanned using a micro computed tomography (micro-CT) scanner. Using appropriate software, a three-dimensional image of the embryo was created, which could be further exploited. The methodology described could be used for the non-destructive examination of the internal structure of the human embryo, and the resulting data can be used as a resource for medical students, gynaecologists, and paediatricians.
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http://dx.doi.org/10.1007/s12565-016-0377-3DOI Listing
March 2017

Identifying Effective Enzyme Activity Targets for Recombinant Class I and Class II Collagenase for Successful Human Islet Isolation.

Transplant Direct 2016 Jan 23;2(1):e54. Epub 2015 Dec 23.

VitaCyte LLC, Indianapolis, IN.

Unlabelled: Isolation following a good manufacturing practice-compliant, human islet product requires development of a robust islet isolation procedure where effective limits of key reagents are known. The enzymes used for islet isolation are critical but little is known about the doses of class I and class II collagenase required for successful islet isolation.

Methods: We used a factorial approach to evaluate the effect of high and low target activities of recombinant class I (rC1) and class II (rC2) collagenase on human islet yield. Consequently, 4 different enzyme formulations with divergent C1:C2 collagenase mass ratios were assessed, each supplemented with the same dose of neutral protease. Both split pancreas and whole pancreas models were used to test enzyme targets (n = 20). Islet yield/g pancreas was compared with historical enzymes (n = 42).

Results: Varying the Wunsch (rC2) and collagen degradation activity (CDA, rC1) target dose, and consequently the C1:C2 mass ratio, had no significant effect on tissue digestion. Digestions using higher doses of Wunsch and CDA resulted in comparable islet yields to those obtained with 60% and 50% of those activities, respectively. Factorial analysis revealed no significant main effect of Wunsch activity or CDA for any parameter measured. Aggregate results from 4 different collagenase formulations gave 44% higher islet yield (>5000 islet equivalents/g) in the body/tail of the pancreas (n = 12) when compared with those from the same segment using a standard natural collagenase/protease mixture (n = 6). Additionally, islet yields greater than 5000 islet equivalents/g pancreas were also obtained in whole human pancreas.

Conclusions: A broader C1:C2 ratio can be used for human islet isolation than has been used in the past. Recombinant collagenase is an effective replacement for the natural enzyme and we have determined that high islet yield can be obtained even with low doses of rC1:rC2, which is beneficial for the survival of islets.
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http://dx.doi.org/10.1097/TXD.0000000000000563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4946501PMC
January 2016

Variability of the pronator teres muscle and its clinical significance.

Rom J Morphol Embryol 2015 ;56(3):1127-35

Department of Anatomy, Faculty of Medicine, Masaryk University Brno, Czech Republic;

While investigating the cause of entrapment syndrome of the peripheral nerves in the elbow region, we observed variability of the pronator teres muscle and the relationship of this muscle to the median nerve and the surrounding vessels. Attention was also paid to the occurrence of the supracondylar process of the humerus and Struthers' ligament with regard to their ontogenetic and phylogenetic development. For this purpose, a classical anatomical dissection of the upper limbs of 68 adults, three fetuses and a phylogenetic assessment of five mammalian species was performed. In terms of variability in the anatomical structures of the elbow region, we found the most serious clinical condition to be where the median nerve ran through the pronator canal together with the ulnar vessels (1.5%), or when it passed through the ulnar head of the pronator teres (5.9%). The pronator teres examined by us in fetuses showed the same arrangement as in adult individuals, including the created ulnar head. The occurrence of a supracondylar process and Struthers' ligament was not observed in our collection. The presence of these structures was not confirmed during the fetal period, either. The phylogenetic part of the study re-opened the question of the meaning and function of the entepicondylar foramen, because we noted differences in the occurrence of this structure in two related genera with a very similar way of life (Djungarian hamster and golden hamster).
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November 2016

Tumors in the 18th and 19th centuries at Brno, Czech Republic.

Anthropol Anz 2013 ;70(4):385-405

Symptoms signs of benign and malignant tumors were observed during paleopathological analysis of skeletal remains from the city of Brno in the Czech Republic. Approximately 1,200 skeletons were examined from two Modern era archaeology sites: the Malá Nová ulice (Little New Street) cemetery and the cemetery of the Nemocnice Milosrdných bratrí (Merciful Brothers' Hospital). Osteomas, usually located on the flat bones of the cranial vault, were the most frequent skeletal pathology. Symptoms signs of malignant tumors were observed in three cases. In one case lytic foci caused by the metastases of a malignant tumor in the soft tissue was observed. Traces of myeloma multiplex were found in two individuals. Living conditions in that period, and possible carcinogenic environmental influences were assessed for the general population of Brno. This report contributes to a better understanding of the historical development of cancers and the clarification of possible causes for their modern increase of incidence.
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http://dx.doi.org/10.1127/0003-5548/2013/0343DOI Listing
April 2014

[Death in the early modern period and medical anthropology].

Sb Pr Filos Fak Brnenske Univ Rada Hist 2002 ;51(49):33-9

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March 2007