Publications by authors named "Leila Larisa Medeiros Marques"

6 Publications

  • Page 1 of 1

Medicinal effects of Peruvian maca (Lepidium meyenii): a review.

Food Funct 2020 Jan;11(1):83-92

Department of Food Engineering, Federal University of Technology - Paraná (UTFPR), Campo Mourão, Paraná 87301-005, Brazil.

Peruvian maca (Lepidium meyenii) is a root native to the Andean region, cultivated for at least 2000 years. Maca is rich in fiber, a large number of essential amino acids, fatty acids, and other nutrients, including vitamin C, copper, iron, and calcium. Besides these essential nutrients, this root contains bioactive compounds responsible for benefits to the human body, which has caused a considerable increase in its consumption in the last 20 years worldwide. This review documents the Peruvian maca composition and the recent findings regarding the medicinal effects of this root in sexual dysfunction regulation, neuroprotective effects, action in memory enhancement, antidepressant, antioxidant, anti-cancer, and anti-inflammatory activities, and skin protection.
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http://dx.doi.org/10.1039/c9fo02732gDOI Listing
January 2020

Substitution of synthetic antioxidant by curcumin microcrystals in mortadella formulations.

Food Chem 2019 Dec 23;300:125231. Epub 2019 Jul 23.

Department of Food Engineering, Federal University of Technology - Paraná (UTFPR), Campo Mourão 87301-005, Brazil.

This research was to compare mortadella elaborated with synthetic antioxidant and microcrystals of curcumin in relation to its physicochemical and sensorial characteristics for a period of 90 days. It was detect no differences between the three evaluated treatments in relation to the pH, color, and texture profile features. The mortadella with curcumin microcrystals showed significantly lower TBARS values at the end of the storage when compared to the other treatments. In the sensory analysis, the addition of curcumin decreased the acceptance of color's sample and the purchase intention, but no significant difference was observed among the other attributes. The color of the sample containing curcumin also became worse than its day-of-production standard during storage. The results obtained suggest the potential of curcumin in replacing synthetic antioxidants in cooked meat sausage, since it practically does not modify its physicochemical characteristics, besides preventing the oxidation of the food.
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http://dx.doi.org/10.1016/j.foodchem.2019.125231DOI Listing
December 2019

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells.

PLoS One 2019 14;14(2):e0212089. Epub 2019 Feb 14.

Programa de Pós-Graduação em Ciências Farmacêuticas, Department of Pharmacy, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.

Alzheimer's disease (AD) is the most common form of dementia and has no cure. Therapeutic strategies focusing on the reduction of oxidative stress, modulation of amyloid-beta (Aβ) toxicity and inhibition of tau protein hyperphosphorylation are warranted to avoid the development and progression of AD. The aim of this study was to screen the crude extracts (CEs) and ethyl-acetate fractions (EAFs) of Guazuma ulmifolia, Limonium brasiliense, Paullinia cupana, Poincianella pluviosa, Stryphnodendron adstringens and Trichilia catigua using preliminary in vitro bioassays (acetylcholinesterase inhibition, antioxidant activity and total polyphenol content) to select extracts/fractions and assess their protective effects against Aβ25-35 toxicity in SH-SY5Y cells. The effect of the EAF of S. adstringens on mitochondrial membrane potential, lipid peroxidation, superoxide production and mRNA expression of 10 genes related to AD was also evaluated and the electropherogram fingerprints of EAFs were established by capillary electrophoresis. Chemometric tools were used to correlate the in vitro activities of the samples with their potential to be evaluated against AD and to divide extracts/fractions into four clusters. Pretreatment with the EAFs grouped in cluster 1 (S. adstringens, P. pluviosa and L. brasiliense) protected SH-SY5Y cells from Aβ25-35-induced toxicity. The EAF of S. adstringens at 15.62 μg/mL was able completely to inhibit the mitochondrial depolarization (69%), superoxide production (49%) and Aβ25-35-induced lipid peroxidation (35%). With respect to mRNA expression, the EAF of S. adstringens also prevented the MAPT mRNA overexpression (expression ratio of 2.387x) induced by Aβ25-35, which may be related to tau protein hyperphosphorylation. This is the first time that the neuroprotective effects of these fractions have been demonstrated and that the electropherogram fingerprints for the EAFs of G. ulmifolia, L. brasiliense, P. cupana, P. pluviosa and S. adstringens have been established. The study expands knowledge of the in vitro protective effects and quality control of the evaluated fractions.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212089PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375598PMC
November 2019

Chemical analysis of the semipurified extract of and evaluation of inhibitory effects against .

Nat Prod Res 2020 Aug 2;34(16):2332-2335. Epub 2019 Jan 2.

Laboratory of Pharmaceutical Biology, Palafito, Department of Pharmacy, State University of Maringá - UEM, Maringá, PR, Brazil.

Kunth, commonly known as guarana, is a native Brazilian plant species from the Amazon area that presents various biological effects, including antimicrobial action. The aim of this study was to chemically analyse the semipurified aqueous extract (AqF) of the plant and to evaluate the activity of crude (CE), ethyl-acetate (EAF), and AqF extracts against . The chemical profile of AqF was determined based on solid analysis C-NMR, direct infusion mass spectrometry (ESI-MS), and MALDI-TOF. The C-NMR spectrum showed characteristics of flavan-3-ol and oligomeric proanthocyanidins. ESI-MS revealed the presence of procyanidin, caffeic acid and its derivatives. MALDI-TOF analysis detected procyanidins of up to 6 units and profisetinidins of up to 5 units. Whereas CE and EAF showed inhibitory activity against , CE, EAF, and AqF presented not high inhibitory activity against urease. The results demonstrate the potential of to control and prevent infection.
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http://dx.doi.org/10.1080/14786419.2018.1533825DOI Listing
August 2020

Evaluation of Antibacterial, Antineoplastic, and Immunomodulatory Activity of Seeds Crude Extract and Ethyl-Acetate Fraction.

Evid Based Complement Alternat Med 2016 7;2016:1203274. Epub 2016 Dec 7.

Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PE, Brazil.

(Guarana) is a native plant of Amazon region that has very traditional importance. Its seeds are rich in bioactive compounds, including tannins, which exhibit relevant properties. This study aimed to evaluate antibacterial, antineoplastic, and immunomodulatory activity of seeds crude extract (CE) and ethyl-acetate fraction (EAF). Antibacterial activity was evaluated by determination of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Antineoplastic activity was evaluated by MTT assays in hepatocellular carcinoma (HepG2), breast adenocarcinoma (MCF-7), ductal carcinoma (T47-D), non-Hodgkin's B cell lymphoma (Toledo), T cell leukemia (Jukart), and Acute Leukemia (HL-60) cell lines. BALB/c mice splenocytes were treated to assess IFN-, IL-6, IL-17, and IL-10 levels by sandwich ELISA. CE and EAF were not toxic to peripheral blood cells and splenocytes. CE and EAF fractions showed a bacteriostatic activity (MIC = 250 g/mL) and presented IC values of 70.25 g/mL and 61.18 g/mL in HL-60 leukemia cell line. All cytokines evaluated had their levels reduced after treatment, following dose-response model. Different biological activities were observed for both CE and EAF, suggesting as a source of bioactive substances, especially tannins that may be used for several diseases treatments.
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http://dx.doi.org/10.1155/2016/1203274DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5174172PMC
December 2016

Guaraná (Paullinia cupana) seeds: Selective supercritical extraction of phenolic compounds.

Food Chem 2016 Dec 15;212:703-11. Epub 2016 Jun 15.

Department of Pharmacy, State University of Maringá, Av. Colombo 5790, BR-87020-900 Maringá, Paraná, Brazil. Electronic address:

Approximately 70% of the Brazilian production of guaraná (Paullinia cupana) seeds is absorbed by the beverage industries. Guaraná has several pharmacological properties: energy stimulant, antimicrobial, chemoprophylactic, antigenotoxic, antidepressive, anxiolytic, and anti-amnesic effects. Supercritical carbon dioxide extraction of bioactive compounds from guaraná seeds was carried out and optimized by an orthogonal array design (OA9(3(4))). The factors/levels studied were: modifier(s) (ethanol and/or methanol), extraction time (20, 40, and 60min), temperature (40, 50, and 60°C), and pressure (100, 200, and 300bar). The statistical design was repeated with increasing proportions of modifiers. The percentage of modifier used was proportional to the amount of polar compounds extracted. The best conditions for the supercritical extraction, based on the content of polyphenols, epicatechin/catechin quantification, yield and operating cost, proved to be: 40% ethanol:methanol during 40min, under 40°C, and 100bar. The temperature had a significant effect on the total phenolic content.
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http://dx.doi.org/10.1016/j.foodchem.2016.06.028DOI Listing
December 2016
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