Publications by authors named "Lei Zhou"

1,791 Publications

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Dynamic fracture mechanics and energy distribution rate response characteristics of coal containing bedding structure.

PLoS One 2021 24;16(6):e0247908. Epub 2021 Jun 24.

School of Energy Science and Engineering, Henan Polytechnic University, Jiaozuo, China.

To investigate the influence of bedding structure and different loading rates on the dynamic fracture characteristics and energy dissipation of Datong coal, a split Hopkinson bar was used to obtain the fracture characteristics of coal samples with different bedding angles. The process of crack initiation and propagation in Datong coal was recorded by the high-speed camera. The formula for the model I fracture toughness of the transversely isotropic material is obtained on the basis of the finite element method (FEM) together with the J-integral. By comparing the incident energy, absorbed energy, fracture energy and residual kinetic energy of Datong coal samples under various impact speeds, the energy dissipation characteristics during the dynamic fracture process of coal considering the bedding structure is acquired. The experimental results indicate that the fracture pattern of notched semi-circular bending (NSCB) Datong coal is tensile failure. After splitting into two parts, the coal sample rotates approximately uniformly around the contact point between the sample and the incident rod. The dynamic fracture toughness is 3.52~8.64 times of the quasi-static fracture toughness for Datong coal. Dynamic fracture toughness increases with increasing impact velocity, and the effect of bedding angle on fracture toughness then decreases. In addition, the residual kinetic energy of coal samples with the same bedding angle increases with the increase of impact speed. The energy utilization rate decreases continuously, and the overall dispersion of statistical data decreases gradually. In rock fragmentation engineering, the optimum loading condition is low-speed loading regardless of energy utilization efficiency or fracture toughness. These conclusions may have significant implications for the optimization of hydraulic fracturing process in coal mass and the further understanding of crack propagation mechanisms in coalbed methane extraction (CME). The anisotropic effect of coal should be fully considered in both these cases.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0247908PLOS
June 2021

A zwitterionic polypeptide nanocomposite with unique NIR-I/II photoacoustic imaging for NIR-I/II cancer photothermal therapy.

J Mater Chem B 2021 Jun 23. Epub 2021 Jun 23.

Joint Research Center for Precision Medicine, Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai Fengxian Central Hospital, Shanghai 201499, P. R. China.

The second near infrared photoacoustic imaging (NIR-II PAI) and photothermal therapy (NIR-II PTT) have attracted wide interest in cancer theranostics because of maximum permission exposure (MPE), deep penetration, and lower scattering and background noise compared to NIR-I counterparts; however, it is imperative to develop biocompatible nanomaterials having NIR-II response. By utilizing multivalent Au-S coordination bonds, we constructed a zwitterionic polypeptide nanocomposite of [email protected] with a suitable size of 48 ± 2 nm, which possessed a strong and broad absorbance at 650-1100 nm and an excellent photothermal conversion efficiency of 49.5%. In vitro biological studies demonstrated that NIR-II PTT within MPE was more effective than NIR-I PTT beyond MPE. Along with X-ray computed tomography and photothermal imaging functions, [email protected] in vivo presented unique NIR-I/II PAI with 2.6-5.9 times signal enhancement compared to the contrast. By single dose and NIR-II irradiation (1064 nm, 1 W cm-2, 10 min), NIR-II PTT within MPE completely eradicated MCF-7 tumors without tissue damage and tumor recurrence within 24 days, inducing a better antitumor efficacy than NIR-I PTT beyond MPE. Importantly, this study provides an innovative method for the fabrication of biocompatible zwitterionic polypeptide nanocomposites with unique NIR-I/II PAI and NIR-II PTT attributes, thus holding great potential for precise cancer theranostics and further clinical transitions.
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http://dx.doi.org/10.1039/d1tb00823dDOI Listing
June 2021

In vitro and in vitro toxicity study of diesel exhaust particles using BEAS-2B cell line and the nematode Caenorhabditis elegans as biological models.

Environ Sci Pollut Res Int 2021 Jun 23. Epub 2021 Jun 23.

The Second Affiliated High School of East China Normal University, Shanghai, 201203, China.

It is well accepted that diesel exhaust particles (DEPs) are highly associated with improper function of organ systems. In this study, DEP toxicity was performed by using in vitro human BEAS-2B cell line and in vivo animal model, namely, Caenorhabditis elegans (C. elegans). The potential toxicity of DEP was assessed by the apical endpoints of BEAS-2B cell line and reflections of C. elegans under exposure scenarios of 0~50 μg mL DEP. With the increase of DEP exposure concentration, microscopic accumulations in the cytoplasm of cell line and intestine of C. elegans were observed. Such invasion of DEP impaired the behaviors of C. elegans as well as its un-exposed offspring and caused significant impeded locomotion. Moreover, the disorders of dopaminergic function were observed simultaneously under DEP exposure, specifically manifested by the decreased transcriptional expression of dat-1. The stress responses instructed by the expression of hsp-16.2 were also increased sharply in TJ375 strain of C. elegans at DEP concentrations of 1 and 10 μg mL. In the case of cellular reactions to DEP exposure, the injuries of membrane integrity and the decreased viability of cell line were simultaneously identified, and reactive oxygen species (ROS), damaged DNA fragment, and upregulated apoptosis were monotonically elevated in cell lines with the increase of DEP concentrations. This study provided a systematic insight into toxicity of DEP both in vivo and vitro, demonstrating that DEP exposure could disturb the stability of cell system and further threat the stability of organism.
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http://dx.doi.org/10.1007/s11356-021-14908-0DOI Listing
June 2021

Insights of fibroblast growth factor receptor 3 aberrations in pan-cancer and their roles in potential clinical treatment.

Aging (Albany NY) 2021 Jun 23;13. Epub 2021 Jun 23.

Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China.

Fibroblast growth factor receptor 3 (FGFR3) alters frequently across various cancer types and is a common therapeutic target in bladder urothelial carcinoma (BLCA) with FGFR3 variants. Although emerging evidence supports the role of FGFR3 in individual cancer types, no pan-cancer analysis is available. In this work, we used the open comprehensive datasets, covering a total of 10,953 patients with 10,967 samples across 32 TCGA cancer types, to identify the full alteration spectrum of FGFR3. FGFR3 abnormal expression, methylation patterns, alteration frequency, mutation location distribution, functional impact, and prognostic implications differed greatly from cancer to cancer. The overall alteration frequency of FGFR3 was relatively low in all cancers. Targetable mutations were mainly detected in BLCA, and S249C, Y373C, G370C, and R248C were hotspot mutations that could be targeted by an FDA approved erdafitinib. Genetic fusions were mainly observed in glioma, followed by BLCA. FGFR3-TACC3 was the most common fusion type which was proposed as novel therapeutic targets in glioma and was targetable with erdafitinib in BLCA. Lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) were two lung cancer subtypes, FGFR3 fusion and hotspot mutation like S249C were observed more commonly in LUSC but not in LUAD. DNA methylation was correlated with the expression of FGFR3 and its downstream genes in some tumors. FGFG3 abnormal expression and alterations exhibited clinical correlations with patient prognosis in several tumors. This work exhibited the full alteration spectrum of FGFR3 and indicated several new clues for their application as potential therapeutic targets and prognostic indicators.
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http://dx.doi.org/10.18632/aging.203175DOI Listing
June 2021

Machine learning-based prediction of survival prognosis in cervical cancer.

BMC Bioinformatics 2021 Jun 16;22(1):331. Epub 2021 Jun 16.

Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China.

Background: Accurately forecasting the prognosis could improve cervical cancer management, however, the currently used clinical features are difficult to provide enough information. The aim of this study is to improve forecasting capability by developing a miRNAs-based machine learning survival prediction model.

Results: The expression characteristics of miRNAs were chosen as features for model development. The cervical cancer miRNA expression data was obtained from The Cancer Genome Atlas database. Preprocessing, including unquantified data removal, missing value imputation, samples normalization, log transformation, and feature scaling, was performed. In total, 42 survival-related miRNAs were identified by Cox Proportional-Hazards analysis. The patients were optimally clustered into four groups with three different 5-years survival outcome (≥ 90%, ≈ 65%, ≤ 40%) by K-means clustering algorithm base on top 10 survival-related miRNAs. According to the K-means clustering result, a prediction model with high performance was established. The pathways analysis indicated that the miRNAs used play roles involved in the regulation of cancer stem cells.

Conclusion: A miRNAs-based machine learning cervical cancer survival prediction model was developed that robustly stratifies cervical cancer patients into high survival rate (5-years survival rate ≥ 90%), moderate survival rate (5-years survival rate ≈ 65%), and low survival rate (5-years survival rate ≤ 40%).
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http://dx.doi.org/10.1186/s12859-021-04261-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207793PMC
June 2021

Continuous-wave near-infrared stimulated-emission depletion microscopy using downshifting lanthanide nanoparticles.

Nat Nanotechnol 2021 Jun 14. Epub 2021 Jun 14.

Department of Chemistry, National University of Singapore, Singapore, Singapore.

Stimulated-emission depletion (STED) microscopy has profoundly extended our horizons to the subcellular level. However, it remains challenging to perform hours-long, autofluorescence-free super-resolution imaging in near-infrared (NIR) optical windows under facile continuous-wave laser depletion at low power. Here we report downshifting lanthanide nanoparticles that enable background-suppressed STED imaging in all-NIR spectral bands (λ = 808 nm, λ = 1,064 nm and λ = 850-900 nm), with a lateral resolution of below 20 nm and zero photobleaching. With a quasi-four-level configuration and long-lived (τ > 100 μs) metastable states, these nanoparticles support near-unity (98.8%) luminescence suppression under 19 kW cm saturation intensity. The all-NIR regime enables high-contrast deep-tissue (~50 μm) imaging with approximately 70 nm spatial resolution. These lanthanide nanoprobes promise to expand the application realm of STED microscopy and pave the way towards high-resolution time-lapse investigations of cellular processes at superior spatial and temporal dimensions.
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http://dx.doi.org/10.1038/s41565-021-00927-yDOI Listing
June 2021

Strong Interfacial Adhesion between the LiS Cathode and a Functional LiPCeSCl Solid-State Electrolyte Endowed Long-Term Cycle Stability to All-Solid-State Lithium-Sulfur Batteries.

ACS Appl Mater Interfaces 2021 Jun 14;13(24):28270-28280. Epub 2021 Jun 14.

Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, P. R. China.

The extrinsic cathode interface between the sulfide electrolyte and the LiS electrode is always ignored in all-solid-state lithium-sulfur batteries. However, the aggregation of the LiS cathode is still observed during cycling. The gradually lost extrinsic contact interface between the cathode and the electrolyte would result in considerable interface resistance and severe capacity decay in the cell due to the lack of efficient electron and ionic conduction at the interface. Herein, a facile dual-doping strategy demonstrates the synthesis of a functional inorganic electrolyte. The obtained LiPCeSCl glass-ceramic electrolyte shows a higher-lithium-ionic conductivity of 3.2 mS cm at room temperature. Further, UV-vis absorption and ex situ scanning electron microscopy studies confirm robust interfacial adhesion between the functional inorganic electrolyte, LiPCeSCl, and the LiS cathode. Thus, a stable extrinsic cathode interface is unprecedently built. Finally, the all-solid-state lithium-sulfur battery based on the LiPCeSCl electrolyte delivers a higher reversible initial capacity of 617 mA h g, a lower interface resistance of 25 Ω cm and much better cycling stability (with a high capacity retention of 89% after 100 cycles) than the pristine LiPS electrolyte.
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http://dx.doi.org/10.1021/acsami.1c06328DOI Listing
June 2021

Non-Coated Rituximab Induces Highly Cytotoxic Natural Killer Cells From Peripheral Blood Mononuclear Cells Autologous B Cells.

Front Immunol 2021 25;12:658562. Epub 2021 May 25.

Department of Cancer Center, The First Hospital of Jilin University, Changchun, China.

Natural killer (NK) cells are becoming valuable tools for cancer therapy because of their cytotoxicity against tumor cells without prior sensitization and their involvement in graft-versus-host disease; however, it is difficult to obtain highly cytotoxic NK cells without adding extra feeder cells. In this study, we developed a new method for obtaining highly cytotoxic NK cells from peripheral blood mononuclear cells (PBMCs) independently of extra feeder cell addition using rituximab not coated on a flask (non-coated rituximab). We found that rituximab could promote both the activation and expansion of NK cells from PBMCs, irrespective of being coated on a flask or not. However, NK cells activated by non-coated rituximab had much greater antitumor activity against cancer cells, and these effects were dependent on autologous living B cells. The antibody-dependent cellular cytotoxicity effect of NK cells activated by non-coated rituximab was also more substantial. Furthermore, these cells expressed higher levels of CD107a, perforin, granzyme B, and IFN-γ. However, there was no difference in the percentage, apoptosis, and cell-cycle progression of NK cells induced by coated and non-coated rituximab. Non-coated rituximab activated NK cells by increasing AKT phosphorylation, further enhancing the abundance of XBP1s. In conclusion, we developed a new method for amplifying NK cells with higher antitumor functions with non-coated rituximab autologous B cells from PBMCs, and this method more efficiently stimulated NK cell activation than by using coated rituximab.
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http://dx.doi.org/10.3389/fimmu.2021.658562DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185348PMC
May 2021

Acid-catalyzed oxidative cross-coupling of acridans with silyl diazoenolates and a Rh-catalyzed rearrangement: two-step synthesis of γ-(9-acridanylidene)-β-keto esters.

Org Biomol Chem 2021 Jun 9. Epub 2021 Jun 9.

School of Chemistry, Sun Yat-Sen University, Guangzhou 510006, China.

A MsOH-catalyzed oxidative cross-coupling of acridans and silyl diazoenolates and a Rh2(OAc)4-catalyzed rearrangement of the resultant diazo products are described. The reactions provide various γ-(9-acridanylidene)-β-keto esters in good yields, which bear an active α-methylene unit for further functionalization.
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http://dx.doi.org/10.1039/d1ob00691fDOI Listing
June 2021

Changes in protist communities in drainages across the Pearl River Delta under anthropogenic influence.

Water Res 2021 Jul 27;200:117294. Epub 2021 May 27.

Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, 510642 Guangzhou, China. Electronic address:

Drainages in the Pearl River Delta urban agglomeration (PRDUA) host vital aquatic ecosystems and face enormous pressures from human activities in one of the largest urban agglomerations in the world. Despite being crucial components of aquatic ecosystems, the interactions and assembly processes of the protistan community are rarely explored in areas with serious anthropogenic disturbance. To elucidate the mechanisms of these processes, we used environmental DNA sequencing of 18S rDNA to investigate the influence of environmental factors and species interactions on the protistan community and its assembly in drainages of the PRDUA during summer. The protistan community showed a high level of diversity and a marked spatial pattern in this region. Community assembly was driven primarily by stochastic processes based on the Sloan neutral community model, explaining 74.28%, 75.82%, 73.67%, 74.40% and 51.24% of community variations in the BJ (Beijiang), XJ (Xijiang), PRD (Pearl River Delta), PRE (Pearl River Estuary) areas and in total, respectively. Meanwhile, environmental variables including temperature, pH, dissolved oxygen, transparency, nutrients and land use were strongly correlated with the composition and assembly of the protistan community, explaining 40.40% of variation in the protistan community. Furthermore, the bacterial community was simultaneously analysed by the 16S rDNA sequencing. Co-occurrence network analysis revealed that species interactions within bacteria (81.41% positive) or protists (82.80% positive), and those between bacteria and protists (50% positive and 50% negative) impacted the protistan community assembly. In summary, stochastic processes dominated, whereas species interactions and environmental factors also played important roles in shaping the protistan communities in drainages across the PRDUA. This study provides insights into the ecological patterns, assembly processes and species interactions underlying protistan dynamics in urban aquatic ecosystems experiencing serious anthropogenic disturbance.
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http://dx.doi.org/10.1016/j.watres.2021.117294DOI Listing
July 2021

Trace level nitrite sensitized photolysis of the antimicrobial agents parachlormetaxylenol and chlorophene in water.

Water Res 2021 Jul 23;200:117275. Epub 2021 May 23.

College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

Nitrite (NO)-sensitized photolysis plays an important role in the attenuation of effluent-derived trace organic contaminants (e.g., anilines, phenolic compounds, etc.) in surface waters. However, the kinetics, mechanisms, and influencing factors of photolysis of many emerging contaminants sensitized by NO still remain largely unknown. Herein, we report that NO-sensitized photolysis of the antimicrobial agents parachlormetaxylenol (PCMX) and chlorophene (CP) in aqueous solution under ultraviolet 365 nm (UV) radiation. A nonlinear increase in photolysis rate constants of PCMX and CP was observed with increasing NO concentration. Radical quenching studies and kinetic modeling revealed that hydroxyl radical (HO•) and nitrogen dioxide radicals (NO) contributed dominantly to the removal of PCMX and CP. Solid phase extraction (SPE) combined with high resolution-mass spectrometry (HR-MS) analysis identified a series of intermediate products including hydroxylated, nitrated, nitrosated, and dimerized derivatives. Experiments with isotopically labelled nitrite (NO) showed that the nitro- and nitroso-substituents of intermediate products were derived from the nitrite nitrogen. Based on the identified products and theoretical computations, the mechanisms and pathways of NO-sensitized photolysis of PCMX and CP are elucidated. Deoxygenation partially inhibited the formation of 4-chloro-3,5-dimethyl-2-nitrophenol (nitro-PCMX) while the presence of HO• scavenger such as isopropanol (i-PrOH) suppressed the further transformation of nitro-PCMX. The presence of Mississippi River natural organic matter (MRNOM) inhibited the removal of PCMX and CP, likely due to light screening and radical quenching. However, appreciable degradation of PCMX and CP was still observed in wastewater and wetland water matrices. Results of this study shed some light on the transformation and fate of PCMX and CP in NO-rich wastewater effluents or effluent-impacted surface waters under solar radiation.
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http://dx.doi.org/10.1016/j.watres.2021.117275DOI Listing
July 2021

Comparison of the retinal vascular network and structure in patients with optic neuritis associated with myelin oligodendrocyte glycoprotein or aquaporin-4 antibodies: an optical coherence tomography angiography study.

J Neurol 2021 Jun 4. Epub 2021 Jun 4.

Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China.

Objective: To compare the retinal vascular network and structure of optic neuritis associated with myelin oligodendrocyte glycoprotein antibodies (MOG-ON) or aquaporin-4 antibodies (AQP4-ON).

Methods: Nineteen patients with MOG-ON (29 eyes), 24 patients with AQP4-ON (43 eyes), and 25 healthy participants (50 eyes) were enrolled. The best-corrected visual acuity (BCVA), mean deviation (MD), retinal nerve fiber layer (RNFL) thickness, parafoveal ganglion cell and inner plexiform layer (GCIPL) thickness, and vessel densities in the peripapillary and parafoveal areas were measured.

Results: The BCVA, RNFL thickness, GCIPL thickness, and vessel densities in the peripapillary and parafoveal areas were significantly decreased in the AQP4-ON and MOG-ON eyes compared with healthy controls (all P < 0.05). There were no significant differences in the MD, RNFL thickness, GCIPL thickness, or vessel densities between the AQP4-ON and MOG-ON eyes (all P > 0.05). However, the BCVA was significantly worse in AQP4-ON eyes than in MOG-ON eyes (P = 0.001). The peripapillary vessel density was significantly correlated with the BCVA and MD in AQP4-ON eyes and with MD in MOG-ON eyes (all P < 0.05).

Conclusions: MOG-ON and AQP4-ON are associated with severe visual dysfunction, as well as retinal structural and vascular damage. The extent of visual dysfunction was strongly correlated with the peripapillary vessel density. Although we found no significant difference in the MD between MOG-ON and AQP4-ON, which are characterized by comparable vascular and structural damage within the peripapillary and parafoveal areas, the BCVA was worse in AQP4-ON eyes than in MOG-ON eyes.
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http://dx.doi.org/10.1007/s00415-021-10609-3DOI Listing
June 2021

The effect of metabolic syndrome on head and neck cancer incidence risk: a population-based prospective cohort study.

Cancer Metab 2021 Jun 3;9(1):25. Epub 2021 Jun 3.

Department of Otolaryngology, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: There are limited evidences clarifying the impact of metabolic syndrome (MS) and its components on head and neck cancer (HNC) incidence risk. We explored the correlation between MS, MS components, and the combined effects of MS and C-reactive protein (CRP) and HNC risk.

Methods: This is a prospective analysis of 474,929 participants from the UK Biobank cohort. Cox proportional hazard regression was utilized to assess the hazard ratio (HR) and 95% confidence interval (CI) and to explore the non-linear correlation between an individual MS component and HNC risk.

Results: Individuals with MS (HR, 1.05; 95%CI, 0.90-1.22) had no higher HNC risk than those without MS. More MS components showed no higher HNC risk. Nevertheless, hyperglycemia (HR, 1.22; 95%CI, 1.02-1.45) was independently correlated with elevated HNC risk. In a non-linear manner, waist circumference and high-density lipoprotein cholesterol (HDL-C) showed a U-shaped association with HNC risk. Further, piecewise linear model analysis indicated that higher male waist circumference, female waist circumference (≥93.16 cm), blood glucose (≥4.70 mmol/L) and male HDL-C (≥1.26mmo/L), and lower male HDL-C (<1.26mmo/L) were correlated with higher HNC risk. Increased CRP (≥1.00mg/dL) elevated HNC risk and individuals with MS and CRP≥1.00mg/dL had the highest HNC risk (HR, 1.29; 95%CI, 1.05-1.58). But no joint effect between MS and CRP was detected (p-interaction=0.501).

Conclusions: MS are not correlated with elevated HNC risk. High waist circumference and blood glucose are independent risk factor of HNC incidence. Controlling HDL-C in an appropriate range can get the lowest risk of male HNC. No joint effect of MS and CRP exists in HNC tumorigenesis.
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http://dx.doi.org/10.1186/s40170-021-00261-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173864PMC
June 2021

Multicolor Coding Up-Conversion Nanoplatform for Rapid Screening of Multiple Foodborne Pathogens.

ACS Appl Mater Interfaces 2021 Jun 2;13(23):26782-26789. Epub 2021 Jun 2.

Institute of Optoelectronics Technology, Key Laboratory of Luminescence and Optical Information, Ministry of Education, Beijing Jiaotong University, Beijing 100044, P. R. China.

Technologies for rapid screening of multiple foodborne pathogens have been urgently needed because of the complex food matrix and high outbreaks of foodborne diseases. In this study, multicolor coding up-conversion nanoparticles (UCNPs) were synthesized and applied for rapid and simultaneous detection of five kinds of foodborne pathogens. The multicolor coding UCNPs were obtained through doping different concentrations of a sensitizer (Yb) on the shell of the synthesized NaYF:Yb, Tm (20%/2%)@NaYF:Yb, and Er ( %/2%) core/shell nanocrystals. All the UCNPs could emit red and green luminescence simultaneously once excited with near-infrared wavelength (980 nm), and the ratio of red and green (R/G ratio) emission light intensity of each kind of UCNPs varied depending on the Yb doping concentration. In addition, the magnetic nanoparticles (MNPs) modified with the monoclonal antibodies (mAbs) against the target bacteria were used to capture and separate the bacteria, resulting in obtaining the MNP-bacterium complexes. Different UCNPs with multicolor coding acted as signal probes were also modified with the mAbs to react with the MNP-bacterium complexes to form the MNP-bacterium-UCNP sandwich complexes. After the sandwich complexes were excited with a wavelength of 980 nm, the obtained R/G ratios and the green photoluminescence intensity (PL intensity) could be used to distinguish and quantitatively detect foodborne pathogens, respectively. This proposed nanoplatform could detect five foodborne pathogens simultaneously within 2 h with good sensitivity and specificity, showing great potential for multiplex detection of other targets in the fields of medical diagnosis and food security.
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http://dx.doi.org/10.1021/acsami.1c05522DOI Listing
June 2021

Identification of an intramolecular switch that controls the interaction of the helicase nsp10 with the membrane-associated nsp12 of porcine reproductive and respiratory syndrome virus.

J Virol 2021 Jun 2:JVI0051821. Epub 2021 Jun 2.

Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.

A critical step in replication of positive-stranded RNA viruses is the assembly of replication and transcription complexes (RTC). We have recently mapped the nonstructural protein (nsp) interaction network of porcine reproductive and respiratory syndrome virus (PRRSV) and provided evidence by truncation mutagenesis that the recruitment of viral core replicase enzymes (nsp9 and nsp10) to membrane proteins (nsp2, nsp3, nsp5 and nsp12) is subject to regulation. Here, we went further to discover an intramolecular switch within the helicase nsp10 that controls its interaction with the membrane-associated protein nsp12. Deletion of nsp10 linker region aa.124-133 connecting the domain 1B to 1A led to complete re-localization and co-localization in the cells co-expressing nsp12. Moreover, single amino acid substitutions (e.g., nsp10 E131A and I132A) were sufficient to enable the nsp10-nsp12 interaction. Further proof came from membrane floatation assays that revealed a clear movement of nsp10 mutants, but not WT nsp10, towards the top of sucrose gradients in the presence of nsp12. Interestingly, the same mutations were not able to activate the nsp10-nsp2/3 interaction, suggesting a differential requirement for conformation. Reverse genetics analysis showed that PRRSV mutants carrying the single substitutions were not viable and defective of subgenomic RNA (sg RNA) accumulation. Together, our results provide strong evidence for a regulated interaction between nsp10 and nsp12 and suggest an essential role for an orchestrated RTC assembly in sg RNA synthesis. Assembly of replication and transcription complexes (RTC) is a limiting step for viral RNA synthesis. The PRRSV RTC macromolecular complexes are comprised of mainly viral nonstructural replicase proteins (nsps), but how they come together remains elusive. We previously showed that viral helicase nsp10 interacts nsp12 in a regulated manner by truncation mutagenesis. Here we revealed that the interaction is controlled by single residues within the domain linker region of nsp10. Moreover, the activation mutations leads to defect in viral sg RNA synthesis. Our results provide important insight into the mechanisms of PRRSV RTC assembly and regulation of viral sg RNA synthesis.
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http://dx.doi.org/10.1128/JVI.00518-21DOI Listing
June 2021

Effects of Ultrasound-Assisted Emulsification on the Emulsifying and Rheological Properties of Myofibrillar Protein Stabilized Pork Fat Emulsions.

Foods 2021 May 26;10(6). Epub 2021 May 26.

College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210046, China.

The current study aimed to investigate the effects of ultrasound-assisted emulsification on the emulsifying and rheological properties of myofibrillar protein (MP) pork fat emulsions under different protein/fat ratios. Changes in emulsion profile, confocal laser scanning microscope images, cryo-scanning microscope images, particle size, protein solubility, surface hydrophobicity and free sulfhydryl groups were determined. Ultrasound significantly increased the emulsifying activity, the emulsifying stability and the flow index for all emulsions, while it decreased the viscosity coefficient of emulsions except for the treatment of protein/fat ratio of 1:15. The results showed that sonication reduced the particle size of the fat particles and evenly distributed the emulsion droplets. Sonication moved the distribution curve of droplet size to the smaller particle size direction and decreased the D3,2 and D4,3 values of emulsion. Sonication resulted in increased bindings between protein hydrophobic groups and fat particles. After ultrasound treatment, more sulfhydryl groups were exposed to aqueous solution, which might decrease the protein solubility in aqueous solution. Ultrasound-assisted emulsification could directly enhance the emulsifying and rheological properties of MP-stabilized pork fat emulsions at different protein/fat ratios, in particular at the ratio of 1:10.
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http://dx.doi.org/10.3390/foods10061201DOI Listing
May 2021

Evolutionary Patterns of Codon Usage in Major Lineages of Porcine Reproductive and Respiratory Syndrome Virus in China.

Viruses 2021 May 31;13(6). Epub 2021 May 31.

Key Laboratory of Animal Epidemiology of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Agricultural University, Beijing 100193, China.

Porcine reproductive and respiratory syndrome virus (PRRSV) is economically important and characterized by its extensive variation. The codon usage patterns and their influence on viral evolution and host adaptation among different PRRSV strains remain largely unknown. Here, the codon usage of ORF5 genes from lineages 1, 3, 5, and 8, and MLV strains of type 2 PRRSV in China was analyzed. A compositional property analysis of ORF5 genes revealed that nucleotide C is most frequently used at the third position of codons, accompanied by rich GC3s. The effective number of codon (ENC) and codon pair bias (CPB) values indicate that all ORF5 genes have low codon bias and the differences in CPB scores among four lineages are almost not significant. When compared with host codon usage patterns, lineage 1 strains show higher CAI and SiD values, with a high similarity to pig, which might relate to its predominant epidemic propensity in the field. The CAI, RCDI, and SiD values of ORF5 genes from different passages of MLV JXA1R indicate no relation between attenuation and CPB or codon adaptation decrease during serial passage on non-host cells. These findings provide a novel way of understanding the PRRSV's evolution, related to viral survival, host adaptation, and virulence.
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http://dx.doi.org/10.3390/v13061044DOI Listing
May 2021

The effect of the synchronized multi-dimensional policies on imported COVID-19 curtailment in China.

PLoS One 2021 1;16(6):e0252224. Epub 2021 Jun 1.

School of Economics and Management, Shanghai Maritime University, Shanghai, China.

As countries are lifting restrictions and resuming international travels, the rising risk of COVID-19 importation remains concerning, given that the SARS-CoV-2 virus could be transmitted unintentionally through the global transportation network. To explore and assess the effective strategies for curtailing the epidemic risk from international importation nationwide, we evaluated "the joint prevention and control" mechanism, which made up of 19 containment policies, on how it impacted the change of medical observation and detection time from border arrival to laboratory confirmation of COVID-19 in its burst in China. Based on 1,314 epidemiological-survey cases from February 29 to May 25, 2020, we found that the synchronized approach of implementing multi-dimensional interventional policies, such as a centralized quarantine and nucleic acid testing (NAT), flight service adjustment and border closure, effectively facilitate early identification of infected case. Specifically, the implementation of the international flight service reduction was found to be associated with a reduction of the mean intervals of diagnosis from arrival to lab-confirmation by 0.44 days maximally, and the border closure was associated with a reduction of the diagnosis interval of imported cases by 0.69 days, from arrival to laboratory confirmation. The study suggests that a timely and synchronized implementation of multi-dimensional policies is compelling in preventing domestic spreading from importation.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0252224PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168853PMC
June 2021

Aspiration dynamics generate robust predictions in heterogeneous populations.

Nat Commun 2021 05 31;12(1):3250. Epub 2021 May 31.

Center for Systems and Control, College of Engineering, Peking University, Beijing, China.

Update rules, which describe how individuals adjust their behavior over time, affect the outcome of social interactions. Theoretical studies have shown that evolutionary outcomes are sensitive to model details when update rules are imitation-based but are robust when update rules are self-evaluation based. However, studies of self-evaluation based rules have focused on homogeneous population structures where each individual has the same number of neighbors. Here, we consider heterogeneous population structures represented by weighted networks. Under weak selection, we analytically derive the condition for strategy success, which coincides with the classical condition of risk-dominance. This condition holds for all weighted networks and distributions of aspiration levels, and for individualized ways of self-evaluation. Our findings recover previous results as special cases and demonstrate the universality of the robustness property under self-evaluation based rules. Our work thus sheds light on the intrinsic difference between evolutionary dynamics under self-evaluation based and imitation-based update rules.
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http://dx.doi.org/10.1038/s41467-021-23548-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166829PMC
May 2021

Lipidomic and Transcriptomic Analysis of the Longissimus Muscle of Luchuan and Duroc Pigs.

Front Nutr 2021 7;8:667622. Epub 2021 May 7.

Teaching and Research Section of Biotechnology, Nanning University, Nanning, China.

Meat is an essential food, and pork is the largest consumer meat product in China and the world. Intramuscular fat has always been the basis for people to select and judge meat products. Therefore, we selected the Duroc, a western lean pig breed, and the Luchuan, a Chinese obese pig breed, as models, and used the longissimus dorsi muscle for lipidomics testing and transcriptomics sequencing. The purpose of the study was to determine the differences in intramuscular fat between the two breeds and identify the reasons for the differences. We found that the intramuscular fat content of Luchuan pigs was significantly higher than that of Duroc pigs. The triglycerides and diglycerides related to flavor were higher in Luchuan pigs compared to Duroc pigs. This phenotype may be caused by the difference in the expression of key genes in the glycerolipid metabolism signaling pathway.
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http://dx.doi.org/10.3389/fnut.2021.667622DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154583PMC
May 2021

Emission of volatile organic compounds during aerobic decomposition of banana peel.

Waste Manag 2021 Jul 27;130:74-81. Epub 2021 May 27.

Institute for Environmental and Climate Research, Jinan University, Guangzhou 510632, China; JNU-QUT Joint Laboratory for Air Quality Science and Management, Jinan University, Guangzhou 511443, China. Electronic address:

Emissions of volatile organic compounds (VOCs) were continuously measured during the aerobic decomposition of banana peel in a laboratory-scale landfill simulator over 25 d. Using direct membrane inlet single-photon ionisation time-of-flight mass spectrometry (MI-SPI-ToF-MS), 18 VOCs belonging to 10 functional groups were detected in the air samples, and their VOC emission profiles were established using cluster analysis on time-resolved data. Three emission stages were clearly identified, with the major release for most VOC compounds occurring during the first 14 d. The emission patterns of the individual compounds were quite similar despite the different release mechanisms. In addition, no apparent increase in temperature was observed inside the simulator during the entire experimental period. We suggest that the volatilisation of the constituents in the waste pile contributed equally to VOC emissions as did the degradation of banana peel via microbial activity. The average emission rate of total VOCs reached 44.3 × 10 mg VOC kg of dry banana peel, with more than half belonging to malodourous substances. The malodourous emissions of the decaying banana peel in an aerobic environment mainly originated from styrene, dimethyl sulphide, and diethyl sulphide, the most common contributors to offensive odourants during food waste biodegradation.
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http://dx.doi.org/10.1016/j.wasman.2021.05.020DOI Listing
July 2021

Purification and biochemical characterization of Msi3, an essential Hsp110 molecular chaperone in Candida albicans.

Cell Stress Chaperones 2021 May 28. Epub 2021 May 28.

Department of Physiology and Biophysics, Virginia Commonwealth University, Richmond, VA, 23298, USA.

Hsp110s are unique and essential molecular chaperones in the eukaryotic cytosol. They play important roles in maintaining cellular protein homeostasis. Candida albicans is the most prevalent yeast opportunistic pathogen that causes fungal infections in humans. As the only Hsp110 in Candida albicans, Msi3 is essential for the growth and infection of Candida albicans. In this study, we have expressed and purified Msi3 in nucleotide-free state and carried out biochemical analyses. Sse1 is the major Hsp110 in budding yeast S. cerevisiae and the best characterized Hsp110. Msi3 can substitute Sse1 in complementing the temperature-sensitive phenotype of S. cerevisiae carrying a deletion of SSE1 gene although Msi3 shares only 63.4% sequence identity with Sse1. Consistent with this functional similarity, the purified Msi3 protein shares many similar biochemical activities with Sse1 including binding ATP with high affinity, changing conformation upon ATP binding, stimulating the nucleotide-exchange for Hsp70, preventing protein aggregation, and assisting Hsp70 in refolding denatured luciferase. These biochemical characterizations suggested that Msi3 can be used as a model for studying the molecular mechanisms of Hsp110s.
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http://dx.doi.org/10.1007/s12192-021-01213-5DOI Listing
May 2021

Risk Factors for Death Among the First 80 543 COVID-19 Cases in China: Relationships Between Age, Underlying Disease, Case Severity, and Region.

Clin Infect Dis 2021 May 27. Epub 2021 May 27.

Hubei Center for Disease Control and Prevention, 6 North Zuodaoquan, Hongshan District, Wuhan 430079, China.

Background: Knowledge of COVID-19 epidemiology remains incomplete and crucial questions persist. We aimed to examine risk factors for COVID-19 death.

Methods: A total of 80 543 COVID-19 cases reported in China, nationwide, through April 8, 2020 were included. Risk factors for death were investigated by Cox proportional hazards regression and stratified analyses.

Results: Overall national case fatality ratio (CFR) was 5.64%. Risk factors for death were older age (≥80: adjusted hazard ratio [aHR]=12.58, 95% confidence interval [CI]=6.78-23.33), presence of underlying disease (aHR=1.33, CI=1.19-1.49), worse case severity (severe: aHR=3.86, CI=3.15-4.73; critical: aHR=11.34, CI=9.22-13.95), and near-epicenter region (Hubei: aHR=2.64, CI=2.11-3.30; Wuhan: aHR=6.35, CI=5.04-8.00). CFR increased from 0.35% (30-39 years) to 18.21% (≥70 years) without underlying disease. Regardless of age, CFR increased from 2.50% for no underlying disease to 7.72% for 1, 13.99% for 2, and 21.99% for ≥3. CFR increased with worse case severity from 2.80% (mild), to 12.51% (severe) and 48.60% (critical) regardless of region. Compared to other regions, CFR was much higher in Wuhan regardless of case severity (mild: 3.83% versus 0.14% in Hubei and 0.03% elsewhere; moderate: 4.60% versus 0.21% and 0.06%; severe: 15.92% versus 5.84% and 1.86%; and critical: 58.57% versus 49.80% and 18.39%).

Conclusions: Older patients regardless of underlying disease and patients with underlying disease regardless of age were at elevated risk of death. Higher death rates near the outbreak epicenter and during the surge of cases reflect the deleterious effects of allowing health systems to become overwhelmed.
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http://dx.doi.org/10.1093/cid/ciab493DOI Listing
May 2021

Vinyldiazo Compounds as 3-Carbon Radical Acceptors: Synthesis of 4-Fluoroacridines via Visible-Light-Promoted Cascade Radical Cyclization.

Authors:
Weiyu Li Lei Zhou

Org Lett 2021 Jun 24;23(11):4279-4283. Epub 2021 May 24.

School of Chemistry, Sun Yat-sen University, Guangzhou 510006, China.

Vinyldiazo reagents were developed as the radical acceptors in a visible-light-promoted sequential radical cyclization reaction, providing a mechanistically distinct pathway to achieve (3 + 3) cyclization. Using N-aryl chlorodifluoromethyl alkynyl ketoimines as the radical precursors, the reaction allows the introduction of a fluorine atom to the acridine skeleton during the construction of both the pyridine and benzene motifs from acyclic building blocks. The resulting 4-fluoroacridines exhibited pronounced fluorescent properties in the solid state.
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http://dx.doi.org/10.1021/acs.orglett.1c01204DOI Listing
June 2021

Intravenous immunoglobulin-based adjuvant therapy for severe COVID-19: a single-center retrospective cohort study.

Virol J 2021 05 21;18(1):101. Epub 2021 May 21.

Department of Critical Care Medicine, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, People's Republic of China.

Objective: Coronavirus disease 2019 (COVID-19) is a major challenge facing the world. Certain guidelines issued by National Health Commission of the People's Repubilic of China recommend intravenous immunoglobulin (IVIG) for adjuvant treatment of COVID-19. However, there is a lack of clinical evidence to support the use of IVIG.

Methods: This single-center retrospective cohort study included all adult patients with laboratory-confirmed severe COVID-19 in the Respiratory and Critical Care Unit of Dabie Mountain Regional Medical Center, China. Patient information, including demographic data, laboratory indicators, the use of glucocorticoids and IVIG, hospital mortality, the application of mechanical ventilation, and the length of hospital stay was collected. The primary outcome was the composite end point, including death and the use of mechanical ventilation. The secondary outcome was the length of hospital stay.

Results: Of the 285 patients with confirmed COVID-19, 113 severely ill patients were included in this study. Compared to the non-IVIG group, more patients in the IVIG group reached the composite end point [12 (25.5%) vs 5 (7.6%), P = 0.008] and had longer hospital stay periods [23.0 (19.0-31.0) vs 16.0 (13.8-22.0), P < 0.001]. After adjusting for confounding factors, differences in primary outcomes between the two groups were not statistically significant (P = 0.167), however, patients in the IVIG group had longer hospital stay periods (P = 0.041).

Conclusion: Adjuvant therapy with IVIG did not improve in-hospital mortality rates or the need for mechanical ventilation in severe COVID-19 patients. Our study does not support the use of immunoglobulin in patients with severe COVID-19 patients.
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http://dx.doi.org/10.1186/s12985-021-01575-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139546PMC
May 2021

Screening and identification of key genes in EBV-associated gastric carcinoma based on bioinformatics analysis.

Pathol Res Pract 2021 Jun 9;222:153439. Epub 2021 Apr 9.

Department of Pathogenic Biology, Qingdao University, School of Basic Medicine, Shandong, 308 NingXia Road, Qingdao, 266021, China. Electronic address:

Epstein-Barr virus (EBV) infection is closely related to gastric carcinoma (GC). In this study, we identified a set of DEGs (different expression genes) between EBVaGC (EBV-associated gastric carcinoma) and EBVnGC (EBV-negative gastric carcinoma) through multiple bioinformatics analysis using the data from GEO (Gene Expression Omnibus) dataset GSE51575, and identified ten hub genes (CXCL10, C3, CXCL9, CXCL11, SST, ICAM1, CHRM2, NPY, GBP5 and GBP1). Therefore, we performed relevant survival analysis and immune infiltration analysis, then verified the mRNA expression in GC cell lines and TCGA database. CXCL11 was finally selected to be a potential biomarker for a better prognosis and tumor infiltrating. This may provide a new view about immune therapy for EBVaGC.
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http://dx.doi.org/10.1016/j.prp.2021.153439DOI Listing
June 2021

Mesoscopic TiNbO cages comprised of nanorod units as high-rate lithium-ion battery anode.

J Colloid Interface Sci 2021 Apr 30;600:111-117. Epub 2021 Apr 30.

Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, PR China. Electronic address:

Benefiting from large tunnel structure, zero strain feature, and excellent pseudocapacitive performance, TiNbO was considered as a potential anode material for lithium-ion batteries (LIBs). Herein, TiNbO cages comprised of nanorod units were elaborately designed. The mesoscopic structure could effectively shorten the ion diffusion pathway, and the big central electrolyte reservoir relieves the concentration polarization of electrolyte. Moreover, the perforated pore feature guarantees competent contact between electrolyte and framework. As the anode of LIBs, the mesoscopic TiNbO cages deliver high reversible capacity (302.5 mAh/g) and rate capability (134.3 mAh/g at 30 A/g). This unique mesoscopic structure holds excellent potential for the electrode design of high-rate and long-life LIBs.
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http://dx.doi.org/10.1016/j.jcis.2021.04.136DOI Listing
April 2021

Effects of ultrasound-assisted vacuum tumbling on the oxidation and physicochemical properties of pork myofibrillar proteins.

Ultrason Sonochem 2021 Jun 4;74:105582. Epub 2021 May 4.

Key Laboratory of Meat Processing and Quality Control, Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address:

The present research aimed to investigate the effects of high-intensity ultrasound (HIU, 20 kHz, 0 W, 100 W, 300 W and 500 W)-assisted vacuum tumbling (UVT) for 60 min and 120 min on the oxidation and physicochemical properties of the pork myofibrillar proteins (MPs). Compared with the vacuum tumbling (VT) groups without the HIU assistance, the carbonyl content increased, while the total sulfhydryl (SH) content was reduced with the increase of HIU power and treatment time (P < 0.05). The reactive SH content was increased significantly after treated by UVT with 300 W compared with the VT group (P < 0.05) regardless of the treatment time. Similarly, the surface hydrophobicity (S), the intrinsic tryptophan intensity, and the solubility in the UVT group (300 W) were remarkably higher than those of the VT group (P < 0.05). In contrast, the α-helix content and the particle size of MPs significantly decreased when the HIU power was at 100 W and 300 W (P < 0.05). The results suggest that UVT treatment could change the structure and physicochemical properties of MPs accompanied by protein oxidation.
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http://dx.doi.org/10.1016/j.ultsonch.2021.105582DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122357PMC
June 2021

Targeting RNA Editing of Antizyme Inhibitor 1: a Potential Oligonucleotide-Based Antisense Therapy for Cancer.

Mol Ther 2021 May 8. Epub 2021 May 8.

Cancer Science Institute of Singapore, National University of Singapore, 14 Medical Drive, Singapore 117599; Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, 4 Medical Drive, Singapore 117594. Electronic address:

Dysregulated adenosine-to-inosine (A-to-I) RNA editing is implicated in various cancers. However, no available RNA editing inhibitors have so far been developed to inhibit cancer-associated RNA editing events. Here we decipher the RNA secondary structure of antizyme inhibitor 1 (AZIN1), one of the best-studied A-to-I editing targets in cancer, by locating its editing site complementary sequence (ECS) at the 3'end of exon 12. Chemically modified antisense oligonucleotides (ASOs) which target the editing region of AZIN1 caused a substantial exon 11 skipping; while ECS-targeting ASOs effectively abolish AZIN1 editing without affecting splicing and translation. We demonstrate that complete 2'-O-methyl (2'-O-Me) sugar ring modification in combination with partial phosphorothioate (PS) backbone modification may be an optimal chemistry for editing inhibition. ASO3.2, which targets the ECS, specifically inhibits cancer cell viability in vitro and tumor incidence and growth in xenograft models. Our results demonstrate that this AZIN1-targeting, ASO-based therapeutics may be applicable to a wide range of tumor types.
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http://dx.doi.org/10.1016/j.ymthe.2021.05.008DOI Listing
May 2021

A novel prognostic model predicts overall survival in patients with nasopharyngeal carcinoma based on clinical features and blood biomarkers.

Cancer Med 2021 Jun 11;10(11):3511-3523. Epub 2021 May 11.

Department Of Pathology Laboratory, Maoming People's Hospital, Maoming, P. R. China.

This study aims to develop and validate a novel prognostic model to estimate overall survival (OS) in nasopharyngeal carcinoma (NPC) patients based on clinical features and blood biomarkers. We assessed the model's incremental value to the TNM staging system, clinical treatment, and Epstein-Barr virus (EBV) DNA copy number for individual OS estimation. We retrospectively analyzed 519 consecutive patients with NPC. A prognostic model was generated using the Lasso regression model in the training cohort. Then we compared the predictive accuracy of the novel prognostic model with TNM staging, clinical treatment, and EBV DNA copy number using concordance index (C-index), time-dependent ROC (tdROC), and decision curve analysis (DCA). Subsequently, we built a nomogram for OS incorporating the prognostic model, TNM staging, and clinical treatment. Finally, we stratified patients into high-risk and low-risk groups according to the model risk score, and we analyzed the survival time of these two groups using Kaplan-Meier survival plots. All results were validated in the independent validation cohort. Using the Lasso regression, we established a prognostic model consisting of 13 variables with respect to patient prognosis. The C-index, tdROC, and DCA showed that the prognostic model had good predictive accuracy and discriminatory power in the training cohort than did TNM staging, clinical treatment, and EBV DNA copy number. Nomogram consisting of the prognostic model, TNM staging, clinical treatment, and EBV DNA copy number showed some superior net benefit. Based on the model risk score, we split the patients into two subgroups: low-risk (risk score ≤ -1.423) and high-risk (risk score > -1.423). There were significant differences in OS between the two subgroups of patients. Similar results were observed in the validation cohort. The proposed novel prognostic model based on clinical features and serological markers may represent a promising tool for estimating OS in NPC patients.
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http://dx.doi.org/10.1002/cam4.3839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178501PMC
June 2021