Publications by authors named "Lei Xiao"

394 Publications

Dysfunctions of the paraventricular hypothalamic nucleus induce hypersomnia in mice.

Elife 2021 Nov 17;10. Epub 2021 Nov 17.

Fudan University, Shanghai, China.

Hypersomnolence disorder (HD) is characterized by excessive sleep, which is a common sequela following stroke, infection or tumorigenesis. HD is traditionally thought to be associated with lesions of wake-promoting nuclei. However, lesions of a single wake-promoting nucleus, or even two simultaneously, did not exert serious HD. Therefore, the specific nucleus and neural circuitry for HD remain unknown. Here, we observed that the paraventricular nucleus of the hypothalamus (PVH) exhibited higher c-fos expression during the active period (23:00) than during the inactive period (11:00) in mice. Therefore, we speculated that the PVH, in which most neurons are glutamatergic, may represent one of the key arousal-controlling centers. By using vesicular glutamate transporter 2 (vglut2) mice together with fiber photometry, multichannel electrophysiological recordings, and genetic approaches, we found that PVH neurons were most active during wakefulness. Chemogenetic activation of PVH neurons induced wakefulness for 9 h, and photostimulation of PVH→parabrachial complex/ventral lateral septum circuits immediately drove transitions from sleep to wakefulness. Moreover, lesioning or chemogenetic inhibition of PVH neurons dramatically decreased wakefulness. These results indicate that the PVH is critical for arousal promotion and maintenance.
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http://dx.doi.org/10.7554/eLife.69909DOI Listing
November 2021

Huge mucinous tubular and spindle cell carcinoma of the kidney: A case report.

Urol Case Rep 2022 Jan 22;40:101914. Epub 2021 Oct 22.

Department of Urology, Changsha Central Hospital, Changsha, 410000, China.

Mucinous tubular and spindle cell carcinoma (MTSCC) of the kidney is a rare type of kidney cancer. Accurate diagnosis depends on pathological examination. A huge left renal tumor was incidentally found by computed tomography (CT) examination of a 52-year-old lady. She underwent Laparoscopic radical left nephrectomy. The postoperative pathological diagnosis is Mucinous tubular and spindle cell carcinoma. Eight months later, she showed no signs of .
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http://dx.doi.org/10.1016/j.eucr.2021.101914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556594PMC
January 2022

Prognostic Value of Elevated Levels of Plasma N-Acetylneuraminic Acid in Patients With Heart Failure.

Circ Heart Fail 2021 Nov 29;14(11):e008459. Epub 2021 Oct 29.

Division of Cardiology, Department of Internal Medicine and Hubei Key Laboratory of Genetics and Molecular Mechanisms of Cardiological Disorders, Tongji Hospital (C.L., L.X., H.W., Z.W., D.H., B.Y., Y.S., X.S., J.H., K.L., M.H., L.N., T.Y., D.W.W.), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Cardiac sialylation is involved in a variety of physiological processes in the heart. Altered sialylation has been implicated in heart failure (HF) mice. However, its role in patients with HF is unclear, and the potential effect of modulation of cardiac sialylation is worth exploring.

Methods: We first assessed the association between plasma N-acetylneuraminic acid levels and the incidence of adverse cardiovascular events in patients with HF over a median follow-up period of 2 years. Next, immunoblot analysis and lectin histochemistry were performed in cardiac tissue to determine the expression levels of neuraminidases and the extent of cardiac desialylation. Finally, the therapeutic impact of a neuraminidase inhibitor was evaluated in animal models of HF.

Results: Among 1699 patients with HF, 464 (27%) died of cardiovascular-related deaths or underwent heart transplantation. We found that the elevated plasma N-acetylneuraminic acid level was independently associated with a higher risk of incident cardiovascular death and heart transplantation (third tertile adjusted hazard ratio, 2.11 [95% CI, 1.67-2.66], <0.001). In addition, in cardiac tissues from patients with HF, neuraminidase expression was upregulated, accompanied by desialylation. Treatment with oseltamivir, a neuraminidase inhibitor, in HF mice infused with isoproterenol and angiotensin II significantly inhibited desialylation and ameliorated cardiac dysfunction.

Conclusions: This study uncovered a significant association between elevated plasma N-acetylneuraminic acid level and an increased risk of a poor clinical outcome in patients with HF. Our data support the notion that desialylation represents an important contributor to the progression of HF, and neuraminidase inhibition may be a potential therapeutic strategy for HF.
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http://dx.doi.org/10.1161/CIRCHEARTFAILURE.121.008459DOI Listing
November 2021

The feasibility and efficacy of perioperative auricular acupuncture technique via intradermal needle buried for postoperative movement-evoked pain after open radical gastrectomy: A randomized controlled pilot trial.

Explore (NY) 2021 Oct 3. Epub 2021 Oct 3.

Department of Nursing, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, 155 Hanzhong Road, Qinhuai District, Nanjing, Jiangsu 210029, China. Electronic address:

Introduction: Auricular acupuncture is widely used in the treatment of pain. Recently, the most commonly used method of auricular acupuncture is to embed an intradermal needle into the skin to enhance analgesia through continuous stimulation. We aimed to explore the efficacy and feasibility of this form of auricular acupuncture in the treatment of postoperative movement-evoked pain.

Methods: This single-blind randomized controlled pilot trial was conducted between 23/8/2019 and 10/1/2020. Forty patients were recruited and randomised to either the control group (n = 20) or the experimental group (n = 20). Patients in the control group received sham auricular acupuncture, while patients in the experimental group received auricular acupuncture. A standard routine analgesia was performed in both groups. The patients with NRS score≥4 were given rescue analgesia. Postoperative pain, use of opioids and other analgesics, postoperative recovery and patient's satisfaction were recorded.

Results: The credibility and feasibility of auricular acupuncture for postoperative pain were high in both groups. After auricular acupuncture, the scores of the postoperative movement-evoked pain had a tendency to decrease, but no significant difference was observed between two groups at any time point (P = 0.234∼0.888). The data on postoperative pain at rest confirmed that no significant difference was observed between two groups within 48 h of surgery (P = 0.134∼0.520), and the postoperative pain at rest scores decreased over time; however, from the third day, the pain at rest scores of the experimental group were decreased, and significant differences were observed between the two groups (P = 0.039∼0.047). As for use of rescue analgesic, total opioid consumption and the incidence of postoperative nausea and vomiting, there were no significant differences between the two groups (P = 0.311, P = 0.101, P = 0.661) . In terms of patients' satisfaction, the score of the experimental group was higher than that of the control group, and a significant difference was observed between the two groups (P = 0.000). As for adverse events, two participants reported pain and one patient reported discomfort at the insertion sites during the process of auricular acupuncture intervention, but they both were minor and tolerable.

Conclusion: Auricular acupuncture may have a relief effect on mild postoperative pain at rest with pain score below 3, suggesting that it may be a feasible adjuvant method to relieve mild pain at rest. However, more multi-centre and large-sample studies are needed to verify this result.
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http://dx.doi.org/10.1016/j.explore.2021.09.007DOI Listing
October 2021

Raman Spectroscopic Characterization of Polymerization Kinetics of Cyanoacrylate Embolic Glues for Vascular Embolization.

Polymers (Basel) 2021 Sep 30;13(19). Epub 2021 Sep 30.

School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian 116024, China.

Endovascular glue embolization is a minimally invasive technique used to selectively reduce or block the blood supply to specific targeted vessels. Cyanoacrylate glues, mixed with radiopaque iodized oil, have been widely used for vascular embolization owing to their rapid polymerization rate, good penetration ability and low tissue toxicity. Nevertheless, in clinical practice, the selection of the glue-oil proportion and the manual injection process of mixtures are mostly based on empirical knowledge of operators, as the crucial physicochemical effect of polymerization kinetics has rarely been quantitatively investigated. In this study, the Raman spectroscopy is used for studying the polymerization kinetics of n-butyl-cyanoacrylate-based glues mixed with an iodized oil. To simulate the polymerization process during embolization, glue-oil mixtures upon contact with a protein ionic solution mimicking blood plasma are manually constructed and their polymerization kinetics are systematically characterized by Raman spectroscopy. The results demonstrate the feasibility of Raman spectroscopy in the characterization of polymerization kinetics of cyanoacrylate-based embolic glues. The polymerization process of cyanoacrylate-based mixtures consists of a fast polymerization phase followed by a slow phase. The propagation velocity and polymerization time primarily depend on the glue concentrations. The commonly used 50% mixture polymerizes 1 mm over ∼21.8 s, while it takes ∼51 min to extend to 5 mm. The results provide essential information for interventional radiologists to help them understand the polymerization kinetics of embolic glues and thus regulate the polymerization rate for effective embolization.
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http://dx.doi.org/10.3390/polym13193362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8512224PMC
September 2021

Nursing students' attitudes toward mobile learning: An integrative review.

Int J Nurs Sci 2021 Oct 21;8(4):477-485. Epub 2021 Aug 21.

School of Nursing, Nanjing University of Chinese Medicine, Nanjing, China.

Purpose: This study aimed to explore whether the attitudes of nursing students toward the use of mobile learning are positive or negative and to identify the factors influencing their attitudes by reviewing the literature.

Methods: Electronic search of six databases, including PubMed, the Cumulative Index of Nursing and Allied Health Literature, ProQuest, Web of Science, EMBASE, and Cochrane Library, was conducted, and relevant references within articles were manually searched. Retrieval time was from inception to October 21, 2020. The literature review was conducted in accordance with the PRISMA guidelines and the integrative review method. The Mixed Method Appraisal Tool (MMAT) was used for quality assessment.

Results: A total of 316 articles were identified, and 18 English-language studies were finally included by reviewing titles, abstracts, and full text. Six quantitative, five qualitative, and seven mixed-method articles related to nursing students' attitudes toward the use of mobile learning were identified. The results showed that most nursing students had positive attitudes toward mobile learning. Although students expressed strong intentions for mobile learning, the actual usage rate in practical settings was low. Several advantageous factors included usefulness, convenience, and ease of use, whereas disadvantageous factors included hardware facility, updated content, and software stability.

Conclusion: Most nursing students have positive attitudes and willingness to mobile learning, but the actual use rate remains low. Advantageous and disadvantageous factors coexist. Further studies are needed to assess how mobile learning improves nursing students' clinical knowledge and improves patient care.
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http://dx.doi.org/10.1016/j.ijnss.2021.08.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488805PMC
October 2021

What Is the Role of Thalamostriatal Circuits in Learning Vocal Sequences?

Front Neural Circuits 2021 22;15:724858. Epub 2021 Sep 22.

Department of Neuroscience, UT Southwestern Medical Center, Dallas, TX, United States.

Basal ganglia (BG) circuits integrate sensory and motor-related information from the cortex, thalamus, and midbrain to guide learning and production of motor sequences. Birdsong, like speech, is comprised of precisely sequenced vocal elements. Learning song sequences during development relies on Area X, a vocalization related region in the medial striatum of the songbird BG. Area X receives inputs from cortical-like pallial song circuits and midbrain dopaminergic circuits and sends projections to the thalamus. It has recently been shown that thalamic circuits also send substantial projections back to Area X. Here, we outline a gated-reinforcement learning model for how Area X may use signals conveyed by thalamostriatal inputs to direct song learning. Integrating conceptual advances from recent mammalian and songbird literature, we hypothesize that thalamostriatal pathways convey signals linked to song syllable onsets and offsets and influence striatal circuit plasticity regulation of cholinergic interneurons (ChIs). We suggest that syllable sequence associated vocal-motor information from the thalamus drive precisely timed pauses in ChIs activity in Area X. When integrated with concurrent corticostriatal and dopaminergic input, this circuit helps regulate plasticity on medium spiny neurons (MSNs) and the learning of syllable sequences. We discuss new approaches that can be applied to test core ideas of this model and how associated insights may provide a framework for understanding the function of BG circuits in learning motor sequences.
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http://dx.doi.org/10.3389/fncir.2021.724858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8493212PMC
September 2021

Experimental demonstration of strong unitary uncertainty relations.

Opt Express 2021 Aug;29(18):29567-29575

Uncertainty relations are one of the most important foundations of quantum physics. In the textbook literatures, uncertainty relations usually refer to the preparation uncertainty. Its original formulation based on variances of two observables limits on the ability to prepare an ensemble of quantum systems for which non-commuting observables will have arbitrary uncertainty. The preparation uncertainty relation has been widely investigated. On the other hand, a unitary operator is a fundamental tenet of quantum theory. Every evolution of a closed quantum system is governed by acting unitary operators on the state of the system and the evolution of an open system can be represented by acting unitary operators on an enlarged system consisting of the quantum system as a subsystem. Therefore, naturally, to understand and quantitatively capture the essence of uncertainty relations for unitary operators is important and timely. Here we report an experimental investigation of a set of uncertainty relations for two unitary operators, which are theoretically derived by using a sequence of fine-grained inequalities. We test these uncertainty relations with single photons and interferometric networks. The unitary uncertainty relation is saturated by any pure qubit state. For higher-dimensional states, it is stronger than the best known bound introduced in the previous literatures. The lower bounds of the unitary uncertainty relations can be even further strengthened by the symmetry of permutation. The experimental findings agree with the predictions of quantum theory and respect the new uncertainty relations.
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http://dx.doi.org/10.1364/OE.438774DOI Listing
August 2021

Design of a side-hole-assisted weakly coupled rectangular ring-core multimode fiber for mode-division-multiplexing networks.

Appl Opt 2021 Aug;60(24):7406-7420

We present a side-hole-assisted weakly coupled polarization-maintaining rectangular ring-core multimode fiber supporting 68 eigenmodes. The rectangular ring-core and side-hole structures are introduced to effectively split eigenmodes. The impact of parameters on mode utilization rate and mode number is evaluated at the wavelength of 1550 nm. We also analyze bending loss and broadband performance of the entire C band, including , , , nonlinearity, and differential mode delay. By optimizing the design parameters, the 68 eigenmodes are completely separated with between adjacent modes larger than 10 over the whole C band. The designed fiber can be used for eigenmode-division multiplexing networks to improve transmission capacity and spectral utilization.
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http://dx.doi.org/10.1364/AO.428678DOI Listing
August 2021

Effect of Material Properties on the Foaming Behaviors of PP-Based Wood Polymer Composites Prepared with the Application of Spherical Cavity Mixer.

Polymers (Basel) 2021 Sep 19;13(18). Epub 2021 Sep 19.

National Special Superfine Powder Engineering Research Center of China, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

For the low weight and high strength, the microcellular extrusion foaming technology was applied in the preparation of polypropylene (PP)-based wood polymer composites, and the spherical cavity mixer was used to construct an experimental platform for the uniform dispersion of wood flour (WF). The effects of PP molecular configuration on the composite properties and cell morphology of samples were also investigated. The experimental results indicated that the application of a spherical cavity mixer with a cavity radius of 5 mm could effectively improve the mixing quality and avoid the agglomeration of WF. In addition, compared with the branched molecule, the linear molecule not only increased the melting temperature by about 10 °C, but also endowed composites with a higher complex viscosity at a shear rate lower than 100 s, which contributed to the cell morphology of more microporous samples.
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http://dx.doi.org/10.3390/polym13183179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472921PMC
September 2021

Delivery of miR-26a Using an Exosomes-Based Nanosystem Inhibited Proliferation of Hepatocellular Carcinoma.

Front Mol Biosci 2021 6;8:738219. Epub 2021 Sep 6.

Department of Oncology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.

MicroRNA (abbreviated miRNA)-based treatment holds great promise for application as clinical antitumor therapy, but good carriers for delivery of the miRNA drug are lacking. Exosomes secreted by mesenchymal stem cells (MSCs) have proved to be safe, and exogenously modified exosomes may potentially represent an excellent drug delivery vehicle. In this study, we designed a delivery nano system using single-stranded variable fragment (scFv)-modified exosomes derived from human cord blood MSCs. Genetic engineering technology was used to obtain anti-Glypican 3 (GPC3) scFv-modified exosomes, which were then loaded with miR-26a mimics through electroporation. Results of electron microscopy and dynamic light scattering indicated that the diameter of the drug-carrying exosomes was about 160 nm. Furthermore, anti-GPC3 scFv-modified exosomes effectively delivered miR-26a to GPC3-positive hepatocellular carcinoma cells, thereby inhibiting cell proliferation and migration by regulating the expression of downstream target genes of miR-26a. The exosomes-based nano system displayed favorable anti-tumor effect with no obvious side effects. Our data provided a new perspective for the use of exosome delivery systems for miRNA-based antitumor therapy.
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http://dx.doi.org/10.3389/fmolb.2021.738219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450326PMC
September 2021

Coupling Electrocatalytic Nitric Oxide Oxidation over Carbon Cloth with Hydrogen Evolution Reaction for Nitrate Synthesis.

Angew Chem Int Ed Engl 2021 Nov 7;60(46):24605-24611. Epub 2021 Oct 7.

State Key Laboratory of Chem/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, China.

NO is a harmful pollutant to the environment. The traditional removal of NO is hindered by the harsh operating conditions and sacrifice of value-added chemicals. Efficient electrocatalytic oxidation of NO was achieved over plasma-treated commercial carbon cloth, serving as a promising anode substitution reaction to couple with the hydrogen evolution reaction without consumption of hydrogen-containing resources. The introduction of carboxyl groups onto the carbon cloth boosted the electrocatalytic activity via the enhancement of NO chemisorption. Only potentials of 1.39 V and 1.07 V were applied to reach the current density of 10 mA cm in neutral and acidic conditions, respectively, which is superior to the state-of-the-art electrocatalysts for oxygen evolution. Energy and environmental concerns on fossil-fuel-derived hydrogen production, ammonia manufacture and nitrate synthesis, are greatly alleviated. This work provides an original strategy to realize the resource utilization of NO, the sustainable nitrate synthesis and hydrogen production in a green and economical way.
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http://dx.doi.org/10.1002/anie.202109905DOI Listing
November 2021

Amido-Functionalized Magnetic Metal-Organic Frameworks Adsorbent for the Removal of Bisphenol A and Tetracycline.

Front Chem 2021 6;9:707559. Epub 2021 Aug 6.

National Special Superfine Powder Engineering Research Center of China, School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing, China.

In this paper, amido-functionalized MOFs with core/shell magnetic particles ([email protected](Al)-NH) was prepared by the solvothermal method and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared (FT-IR), Vibrating Sample Magnetometer (VSM) and UV/VIS spectrophotometer. The influence of different factors on the adsorption effect of the pollutant, including adsorbent amounts, adsorption time, ionic strength and pH, were explored. It was found that the amine-decorated [email protected](Al)-NH were efficient for removal of contaminant, with the adsorption capacity for bisphenol A (234.1 mg/g) and tetracycline (84.8 mg/g) under the optimized conditions. The adsorption kinetics and the equilibrium adsorption data indicated that the adsorption process of BPA and TC was more compatible with the pseudo-second-order kinetic model and the Langmuir model, respectively. The thermodynamic values show the adsorption of the mentioned contaminant was spontaneous and endothermic. Moreover, the [email protected](Al)-NH adsorbent had good regeneration and reusability capacity after five cyclic utilization. All these results show [email protected](Al)-NH adsorbent could be a potential candidate for future water purification.
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http://dx.doi.org/10.3389/fchem.2021.707559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8377470PMC
August 2021

Diagnosis and Treatment of Right Heart Failure in Pulmonary Vascular Diseases: A National Heart, Lung, and Blood Institute Workshop.

Circ Heart Fail 2021 06 15;14(6). Epub 2021 Jun 15.

Division of Lung Diseases, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD.

Right ventricular dysfunction is a hallmark of advanced pulmonary vascular, lung parenchymal, and left heart disease, yet the underlying mechanisms that govern (mal)adaptation remain incompletely characterized. Owing to the knowledge gaps in our understanding of the right ventricle (RV) in health and disease, the National Heart, Lung, and Blood Institute (NHLBI) commissioned a working group to identify current challenges in the field. These included a need to define and standardize normal RV structure and function in populations; access to RV tissue for research purposes and the development of complex experimental platforms that recapitulate the environment; and the advancement of imaging and invasive methodologies to study the RV within basic, translational, and clinical research programs. Specific recommendations were provided, including a call to incorporate precision medicine and innovations in prognosis, diagnosis, and novel RV therapeutics for patients with pulmonary vascular disease.
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http://dx.doi.org/10.1161/CIRCHEARTFAILURE.120.007975DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375628PMC
June 2021

CircFAM120B Blocks the Development of Colorectal Cancer by Activating TGF-Beta Receptor II Expression via Targeting miR-645.

Authors:
You Yu Xiao Lei

Front Cell Dev Biol 2021 26;9:682543. Epub 2021 Jul 26.

Department of General Surgery, The First Hospital Affiliated to AMU (Southwest Hospital), Chongqing, China.

Circular RNAs (circRNAs) are implicated in various human cancers, including colorectal cancer (CRC). The objective of this study was to investigate the function and regulatory mechanism of a novel circRNA, circFAM120B, in CRC development. The expression of circFAM120B, miR-645 and TGF-beta receptor II (TGFBR2) mRNA was detected by quantitative real-time polymerase chain reaction. Cellular biological functions, including cell proliferation, migration/invasion, and glycolysis metabolism, were assessed using CCK-8 assay, colony formation assay, transwell assay, and glycolysis stress test, respectively. Glycolysis progression was also monitored by lactate production and glucose consumption. The expression of glycolysis-related markers and TGFBR2 at the protein level was detected by western blot. The interaction between miR-645 and circFAM120B or TGFBR2 was predicted by bioinformatics analysis and verified by pull-down assay, dual-luciferase reporter assay and RIP assay. animal experiments were performed to further explore the function of circFAM120B. The expression of circFAM120B was decreased in CRC tissues and cells. CircFAM120B overexpression blocked CRC cell proliferation, migration/invasion, and glycolysis metabolism. MiR-645 was a target of circFAM120B, and miR-645 restoration reversed the effects of circFAM120B overexpression. In addition, TGFBR2 was a target of miR-645, and miR-645 inhibition-suppressed CRC cell proliferation, migration/invasion and glycolysis were restored by TGFBR2 knockdown. Moreover, circFAM120B activated the expression of TGFBR2 by targeting miR-645. TGFBR2 also blocked tumor growth by targeting the miR-645/TGFBR2 axis. CircFAM120B inhibited CRC progression partly by mediating the miR-645/TGFBR2 network, which explained the potential mechanism of circFAM120B function in CRC.
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http://dx.doi.org/10.3389/fcell.2021.682543DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350741PMC
July 2021

The Prognostic Value of Long Non-Coding RNA SNHG7 in Human Cancer: A Meta-Analysis.

Curr Pharm Biotechnol 2021 Aug 9. Epub 2021 Aug 9.

Department of Gastrointestinal Surgery, Xiangya Hospital of Central South University, Changsha 410000, China.

Background: The long non-coding RNA SNHG7 is upregulated in many types of cancer and plays a role as an oncogene. However, its overall predictive ability in human cancer prognosis has not been assessed using existing databases. Therefore, further study of its prognostic value and clinical significance in human malignancies is warranted.

Methods: We systematically collected relevant literature from multiple electronic document databases about the relationship between SNHG7 expression level and prognosis in patients with solid cancers. We further screened them for eligibility. Pooled hazard ratios (HRs) with 95% confidence intervals (CIs) were used to assess the prognostic value. Odds ratios (ORs) and their 95% CIs were collected to evaluate the relationship between the expression of SNHG7 and clinicopathological features, including lymph node metastasis (LNM), tumour size, tumour node metastasis (TNM) stage and histological grade.

Results: Fourteen original studies involving 971 patients were enrolled strictly following the inclusion and exclusion criteria. The meta-analysis showed that SNHG7 expression was significantly correlated with poor overall survival (HR = 1.93, 95% CI: 1.64-2.26, p<0.001) in human cancer patients. In addition, the pooled OR indicated that overexpression of SNHG7 was associated with earlier LNM (OR = 1.83, 95% CI: 1.44-2.32; P <0.001), and advanced TNM stage (OR = 1.82, 95% CI: 1.44-2.30; P <0.001).Meanwhile, there was no significant heterogeneity between the selected studies, proving the reliability of the meta-analysis results.

Conclusions: High SNHG7 expression may predict poor oncological outcomes in patients with multiple human cancers, which could be a novel prognostic biomarker of unfulfilled clinicopathological features. However, further high-quality studies are needed to verify and strengthen the clinical value of SNHG7 in different types of cancer.
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http://dx.doi.org/10.2174/1389201022666210810100607DOI Listing
August 2021

miR-3187-3p enhances migration and invasion by targeting PER2 in head and neck squamous cell carcinomas.

J Cancer 2021 26;12(17):5231-5240. Epub 2021 Jun 26.

Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan 410008, China.

Invasion and metastasis are major contributors to treatment failure in patients with head and neck squamous cell carcinomas (HNSCCs) and microRNAs (miRNAs) are reported to play important roles in tumor progression. Our study therefore try to find the crucial miRNAs and reveal their molecular and functional mechanisms involved in migration and invasion of HNSCCs. Through The Cancer Genome Atlas (TCGA) data analysis, we screened out miR-3187-3p and its biological function and specific mechanism were further analyzed. The wound-healing and transwell invasion assay demonstrated that miR-3187-3p promoted the capacity of migration and invasion of HNSCCs . Luciferase reporter assays showed that PER2 was a direct target of miR-3187-3p, which could reverse the effect of miR-3187-3p in HNSCCs. Furthermore, we found that miR-3187-3p / PER2 axis activated the Wnt / β-catenin signaling pathway in HNSCCs. Altogether, our study indicated that miR-3187-3p enhanced migration and invasion by targeting PER2 in HNSCCs.
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http://dx.doi.org/10.7150/jca.58593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317515PMC
June 2021

RBM20 Is a Candidate Gene for Hypertrophic Cardiomyopathy.

Can J Cardiol 2021 Jul 29. Epub 2021 Jul 29.

Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Hubei Key Laboratory of Genetics and Molecular Mechanism of Cardiologic Disorders, Huazhong University of Science and Technology, Wuhan, China. Electronic address:

Background: The genetic basis of a considerable fraction of hypertrophic cardiomyopathy (HCM) cases remains unknown. Whether the gene encoding RNA binding motif protein 20 (RBM20) is implicated in HCM and the correlation of clinical characteristics of RBM20 heterozygotes with HCM remain unresolved. We aimed to investigate the association between RBM20 variants and HCM.

Methods: We compared rare variants in the RBM20 gene by exome sequencing in 793 patients with HCM and 414 healthy controls. Based on a case-control approach, we used optimal sequence kernel association test (SKAT-O) to explore whether RBM20 is associated with HCM. The genetic distribution of RBM20 rare variants was then compared between HCM heterozygotes and dilated cardiomyopathy (DCM) heterozygotes. Clinical features and prognosis of RBM20 heterozygotes were compared with nonheterozygotes.

Results: Gene-based association analysis implicated RBM20 as a susceptibility gene for developing HCM. Patients with RBM20 variants displayed a higher prevalence of sudden cardiac arrest (SCA) (6.7% vs 0.9%, P = 0.001), increased sudden cardiac death (SCD) risk factor counts and impaired left ventricle systolic function. Further survival analysis revealed that RBM20 heterozygotes had higher incidences of resuscitated cardiac arrest, recurrent nonsustained ventricular tachycardia, and malignant arrhythmias. Mendelian randomization suggested that RBM20 expression in the left ventricle was causally associated with HCM and DCM with opposite effects.

Conclusions: This study identified RBM20 as a potential causal gene of HCM. RBM20 variants are associated with increased risk for SCA in HCM.
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http://dx.doi.org/10.1016/j.cjca.2021.07.014DOI Listing
July 2021

PTSD Among Healthcare Workers During the COVID-19 Outbreak: A Study Raises Concern for Non-medical Staff in Low-Risk Areas.

Front Psychiatry 2021 12;12:696200. Epub 2021 Jul 12.

Department of Medical Psychology, Naval Medical University, Shanghai, China.

To investigate the prevalence of sleep quality and post-traumatic stress disorder (PTSD) symptoms of healthcare workers (HCWs) and identify the determinants for PTSD symptoms among HCWs in high-risk and low-risk areas during the COVID-19 outbreak in China. The Pittsburgh Sleep Quality Index and the Impact of Event Scale were used to assess sleep quality and symptoms of PTSD of 421 Chinese HCWs, respectively, from January 30 to March 2, 2020. The influencing factors of PTSD symptoms were identified by univariate analysis and multiple regression. The incidence of HCWs getting PTSD symptoms were 13.2%. HCWs from high-risk areas had significantly poorer sleep quality ( < 0.001). Poor sleep quality was the risk factor of PTSD symptoms for HCWs from high-risk ( = 0.018) and low-risk areas ( < 0.001). Furthermore, non-medical staff were found to be the risk factor for PTSD symptoms only in low-risk areas. HCWs in Hubei had poorer sleep quality. Non-medical HCWs from low-risk areas were associated with more severe PTSD symptoms. Mental health programs should be considered for HCWs, especially those who are often overlooked.
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http://dx.doi.org/10.3389/fpsyt.2021.696200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310947PMC
July 2021

Molecular characterization of a catalase gene from the green peach aphid (Myzus persicae).

Arch Insect Biochem Physiol 2021 Oct 26;108(2):e21835. Epub 2021 Jul 26.

Anhui Provincial Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei, China.

The green peach aphid, Myzus persicae (Sulzer), is a serious agricultural pest with a worldwide distribution. Catalase (CAT), which is encoded by the catalase (Cat) gene, is an extremely important antioxidant enzyme that plays a pivotal role in protecting cells against the toxic effects of hydrogen peroxide. The Cat gene has not been characterized in M. persicae; therefore, this study describes the identification of the Cat (MpCat) gene from M. persicae. MpCat contains an open reading frame of 1515 bp and encodes a MpCAT protein consisting of 504 amino-acid residues. MpCAT possesses features typical of other insect catalases, including 7 conserved amino acids involved in binding heme and 15 involved in binding nicotinamide adenine dinucleotide phosphate. Phylogenetic analysis showed that MpCAT was closely related to orthologs from other aphid species. MpCat consisted of nine exons and eight introns, and the number and insertion sites of introns are consistent with those of Cat genes from Acyrthosiphon pisum (Harris) and Aphis gossypii Glover. The mRNA transcripts of MpCat were detected at all tested developmental stages, with the highest mRNA level in alate adults. The expression of MpCat was significantly upregulated when M. persicae was exposed to low and high temperatures, ultraviolet radiation, Beauveria bassiana, and permethrin. The transcription of MpCat and the activity of catalase were suppressed by RNA interference, and knockdown of MpCat significantly reduced the survival rate in M. persicae under heat stress. The results provide valuable information for further study on the physiological functions of MpCat.
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http://dx.doi.org/10.1002/arch.21835DOI Listing
October 2021

Whole-exome sequencing reveals genetic risks of early-onset sporadic dilated cardiomyopathy in the Chinese Han population.

Sci China Life Sci 2021 Jul 22. Epub 2021 Jul 22.

Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

To reveal genetic risks of early-onset sporadic dilated cardiomyopathy (DCM) patients in the Chinese Han population, we enlisted 363 DCM cases and 414 healthy controls. Whole-exome sequencing and phenotypic characterization were conducted. In total, we identified 26 loss-of-function (LOF) candidates and 66 pathogenic variants from 33 genes, most of which were novel. The deleterious variants can account for 25.07% (91/363) of all patients. Furthermore, rare missense variants in 21 genes were found to be significantly associated with DCM in burden tests. Other than rare variants, twelve common SNPs were significantly associated with an increased risk of DCM in allele-based genetic model association analysis. Of note, in the cumulative risk model, high-risk subjects had a 3.113-fold higher risk of developing DCM than low-risk subjects. Also, DCM in the high-risk group had a younger age of onset than that in the low-risk group. In terms of cardiac function, the mean left ventricular ejection fraction of patients with the deleterious variants was lower than those without (27.73%±10.02% vs. 30.61%±10.85%, P=0.026). To conclude, we mapped a comprehensive atlas of genetic risks in Chinese patients with DCM that might lead to new insights into the mechanisms and risk stratification for DCM.
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http://dx.doi.org/10.1007/s11427-020-1951-4DOI Listing
July 2021

Simulating Exceptional Non-Hermitian Metals with Single-Photon Interferometry.

Phys Rev Lett 2021 Jul;127(2):026404

Beijing Computational Science Research Center, Beijing 100084, China.

We experimentally simulate in a photonic setting non-Hermitian (NH) metals characterized by the topological properties of their nodal band structures. Implementing nonunitary time evolution in reciprocal space followed by interferometric measurements, we probe the complex eigenenergies of the corresponding NH Bloch Hamiltonians, and study in detail the topology of their exceptional lines (ELs), the NH counterpart of nodal lines in Hermitian systems. We focus on two distinct types of NH metals: two-dimensional systems with symmetry-protected ELs, and three-dimensional systems possessing symmetry-independent topological ELs in the form of knots. While both types feature open Fermi surfaces, we experimentally observe their distinctions by analyzing the impact of symmetry-breaking perturbations on the topology of ELs.
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http://dx.doi.org/10.1103/PhysRevLett.127.026404DOI Listing
July 2021

Genetic polymorphisms and haplotypes of ERCC1 and ERCC2 associated with quality of life, depression, and anxiety status among patients with lung cancer.

BMC Cancer 2021 Jul 21;21(1):842. Epub 2021 Jul 21.

Department of Medical Psychology, College of Psychology, Naval Medical University, 800 Xiangyin Rd., Shanghai, 200433, China.

Background: Patients with lung cancer (LC) have a poor quality of life (QoL) and easily suffer from psychological diseases. Previous studies focused less on the relationship between genetic factors and QoL, depression, and anxiety status in LC patients. The current study is intended to explore the relationship between SNPs and haplotypes of ERCC1 and ERCC2 and the QoL, depression and anxiety status of patients with LC.

Methods: QoL, depression and anxiety status were assessed in 291 LC patients using the European Organization for Research and Treatment of Cancer (EORTC) Core Quality of Life Questionnaire (QLQ-C30), EORTC Quality of Life Questionnaire-Lung Cancer 13 (QLQ-LC13), SDS and SAS. Nine tag SNPs of ERCC1 and ERCC2 were detected using an improved multiplex ligation detection reaction (iMLDR) technique. Haplotype analysis was conducted using the software Haploview 4.2. The association between SNPs or haplotypes and QoL or depression or anxiety in LC patients was analyzed by regression analysis.

Results: ERCC1 rs11615 was associated with emotional functioning (P = 0.027), and ERCC1 rs3212986 was associated with anxiety scores (P = 0.018). ERCC1 rs762562-rs3212986 haplotype was associated with cognitive function (P = 0.029), somatic function (P = 0.014) and dysphagia (OR = 3.32, P = 0.044). Patients with ERCC1 rs3212986-rs11615 AG haplotype had worse cognitive function (adjusted Beta = - 5.42) and somatic function (adjusted Beta = - 6.55) and had severer symptoms of loss of appetite (adjusted OR = 1.67) and dysphagia (adjusted OR = 4.43) (All adjusted P < 0.05). ERCC2 rs13181-rs3916874-rs238416 haplotype was associated with emotional functioning (P = 0.035), pain at other sites (OR 1.88, P = 0.014), chest pain (OR 0.42, P = 0.02), dysphagia (OR 2.82, P = 0.048), and anxiety status (OR 0.23, P = 0.009).

Conclusion: After adjustment for environmental factors, SNPs and haplotypes of ERCC1 and ERCC2 were associated with different domains of QoL, depression and anxiety in LC patients.
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http://dx.doi.org/10.1186/s12885-021-08570-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8293557PMC
July 2021

Sodium-Glucose Cotransporter-2 Inhibitors in Vascular Biology: Cellular and Molecular Mechanisms.

Cardiovasc Drugs Ther 2021 12 17;35(6):1253-1267. Epub 2021 Jul 17.

Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, and Institute of Cardiovascular Science, Peking University Health Science Center, Beijing, 100191, China.

Sodium-glucose cotransporter-2 (SGLT2) inhibitors are new antidiabetic drugs that reduce hyperglycemia by inhibiting the glucose reabsorption in renal proximal tubules. Clinical studies have shown that SGLT2 inhibitors not only improve glycemic control but also reduce major adverse cardiovascular events (MACE, cardiovascular and total mortality, fatal or nonfatal myocardial infarction or stroke) and hospitalization for heart failure (HF), and improve outcome in chronic kidney disease. These cardiovascular and renal benefits have now been confirmed in both diabetes and non-diabetes patients. The precise mechanism(s) responsible for the protective effects are under intensive investigation. This review examines current evidence on the cardiovascular benefits of SGLT2 inhibitors, with a special emphasis on the vascular actions and their potential mechanisms.
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http://dx.doi.org/10.1007/s10557-021-07216-9DOI Listing
December 2021

GSTP1 as a novel target in radiation induced lung injury.

J Transl Med 2021 07 8;19(1):297. Epub 2021 Jul 8.

Department of Radiation Oncology, The Fifth Medical Center of the Chinese PLA General Hospital , Beijing, China.

The glutathione S-transferase P1(GSTP1) is an isoenzyme in the glutathione-S transferases (GSTs) enzyme system, which is the most abundant GSTs expressed in adult lungs. Recent research shows that GSTP1 is closely related to the regulation of cell oxidative stress, inhibition of cell apoptosis and promotion of cytotoxic metabolism. Interestingly, there is evidence that GSTP1 single nucleotide polymorphisms (SNP) 105Ile/Val related to the risk of radiation induced lung injury (RILI) development, which strongly suggests that GSTP1 is closely associated with the occurrence and development of RILI. In this review, we discuss our understanding of the role of GSTP1 in RILI and its possible mechanism.
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http://dx.doi.org/10.1186/s12967-021-02978-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268607PMC
July 2021

Nuclear Transglutaminase 2 interacts with topoisomerase II⍺ to promote DNA damage repair in lung cancer cells.

J Exp Clin Cancer Res 2021 Jul 5;40(1):224. Epub 2021 Jul 5.

Department of Radiation Medicine, Faculty of Naval Medicine, Naval Medical University, 800, Xiangyin Road, 200433, Shanghai, P.R. China.

Background: To block repairs of DNA damages, especially the DNA double strand break (DSB) repair, can be used to induce cancer cell death. DSB repair depends on a sequential activation of DNA repair factors that may be potentially targeted for clinical cancer therapy. Up to now, many protein components of DSB repair complex remain unclear or poorly characterized. In this study, we discovered that Transglutaminase 2 (TG2) acted as a new component of DSB repair complex.

Methods: A bioinformatic analysis was performed to identify DNA damage relative genes from dataset from The Cancer Genome Atlas. Immunofluorescence and confocal microscopy were used to monitor the protein localization and recruitment kinetics. Furthermore, immunoprecipitation and mass spectrometry analysis were performed to determine protein interaction of both full-length and fragments or mutants in distinct domain. In situ lung cancer model was used to study the effects cancer therapy in vivo.

Results: After DSB induction, cytoplasmic TG2 was extensively mobilized and translocated into nucleus after phosphorylated at T162 site by DNA-PKcs. Nuclear TG2 quickly accumulated at DSB sites and directly interacting with Topoisomerase IIα (TOPOIIα) with its TGase domain to promote DSB repair. TG2 deficient cells lost capacity of DSB repair and become susceptible to ionizing radiation. Specific inhibition of TG2-TOPOIIα interaction by glucosamine also significantly inhibited DSB repair, which increased sensitivity in lung cancer cells and engrafted lung cancers.

Conclusions: These findings elucidate new mechanism of TG2 in DSB repair trough directly interacting with TOPOIIα, inhibition of which provided potential target for overcoming cancer resistance.
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http://dx.doi.org/10.1186/s13046-021-02009-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258933PMC
July 2021

Endoplasmic Reticulum and Its Significance in Periodontal Disease.

Chin J Dent Res 2021 Jun;24(2):79-84

The endoplasmic reticulum has emerged as a modulator that is essential for cellular homeostasis and human health. It is an extensive membranous organelle that acts as a hub for the physiological and pathological processes. In recent years, it has become a topic of interest in studies on the relationship between endoplasmic reticulum homeostasis and system diseases. Periodontal disease is a prevalent chronic disease that affects tooth-supporting tissues, initiated by the interaction between pathogenic bacterial infection and immune defence and resulting in tooth loss. The endoplasmic reticulum participates in the responses to the fluctuating microenvironments in periodontal pathogenesis and regulates periodontal homeostasis. In this review, we present an overview of the significance of endoplasmic reticulum regulation as a multidimensional mediator in periodontal disease and highlight the potential strategies for periodontal regeneration.
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http://dx.doi.org/10.3290/j.cjdr.b1530481DOI Listing
June 2021

Significantly Enhanced Thermal Decomposition of Mechanically Activated Ammonium Perchlorate Coupling with Nano Copper Chromite.

ACS Omega 2021 Jun 10;6(24):16110-16118. Epub 2021 Jun 10.

National Special Superfine Powder Engineering Research Center of China, School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

In this article, nano-CuCrO (copper chromite)/ultrafine ammonium perchlorate (AP) composites were prepared by a ultrasonic dispersion method and a mechanical grinding method. A series of nano-CuCrO/ultrafine AP composites with different dispersions were prepared by controlling the compounding time to study the best catalytic effect of nano-CuCrO on the ultrafine AP. The microstructures, surface elements, and morphologies of samples were analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and energy dispersion X-ray spectroscopy. The catalytic effect of nano-CuCrO on the thermal decomposition of AP was investigated by differential scanning calorimetric techniques and thermogravimetric analysis. The results indicated that the mechanical ball milling method could make nano-CuCrO more evenly dispersed on the ultrafine AP, and with the increase in the milling time, the uniformity of nano-CuCrO on the ultrafine AP was better. When the milling time was 6-12 h, nano-CuCrO was most evenly dispersed on the ultrafine AP. At this time, the decomposition temperature and Gibbs free energy of the nano-CuCrO/ultrafine AP composite were the lowest, which decreased by 78.1 °C and 25.16 kJ/mol compared with those of ultrafine AP, respectively. Moreover, the mechanical sensitivity of nano-CuCrO/ultrafine AP composites was lower than that of ultrafine AP. It showed that ball milling for 6-12 h could make nano-CuCrO evenly dispersed on the ultrafine AP, and nano-CuCrO could play the best catalytic effect on the ultrafine AP.
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http://dx.doi.org/10.1021/acsomega.1c02002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8223405PMC
June 2021
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