Publications by authors named "Lei Wu"

1,167 Publications

  • Page 1 of 1

Identification of MDM2, YTHDF2 and DDX21 as potential biomarkers and targets for treatment of type 2 diabetes.

Biochem Biophys Res Commun 2021 Oct 9;581:110-117. Epub 2021 Oct 9.

Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, China; Center for Diabetes and Metabolism Research, Division of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, China. Electronic address:

Type 2 diabetes (T2D) is a multifactorial and polygenetic disease, although its exact etiology remains poorly understood. The objective of this study was to identify key biomarkers and potential molecular mechanisms in the development of T2D. Human RNA-Seq datasets across different tissues (GSE18732, GSE41762, and GSE78721) were collected from the Gene Expression Omnibus (GEO) database and differentially expressed genes (DEGs) between T2D and controls were identified using differential analysis. A total of 90 overlapping DEGs were identified, among which YTHDF2, DDX21, and MDM2 were considered as key genes due to their central positions in the PPI network and the same regulatory pattern in T2D. Logistic regression analysis showed that low expression of the key genes increased the risk of T2D. Enrichment analysis revealed that the key genes are involved in various important biological functions and signaling pathways including Notch, Fork head box O (FOXO), and phosphoinositide 3-kinase (PI3K)-Akt. RT-qPCR and Western blot analysis showed that all three key genes were down-regulated in pancreatic islets of both prediabetic and diabetic mouse models. Finally, the insulin-sensitizer, pioglitazone was used to treat db/db mice and immunofluorescence analysis showed that the expression of all three key genes was significantly down-regulated in db/db islets, an effect that was overcome by pioglitazone treatment. Together, these results suggest that the identified key genes could be involved in the development of T2D and serve as potential biomarkers and therapeutic targets for this disease.
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http://dx.doi.org/10.1016/j.bbrc.2021.10.019DOI Listing
October 2021

Ultra-selective molecular-sieving gas separation membranes enabled by multi-covalent-crosslinking of microporous polymer blends.

Nat Commun 2021 Oct 22;12(1):6140. Epub 2021 Oct 22.

State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan, 030001, China.

High-performance membranes exceeding the conventional permeability-selectivity upper bound are attractive for advanced gas separations. In the context microporous polymers have gained increasing attention owing to their exceptional permeability, which, however, demonstrate a moderate selectivity unfavorable for separating similarly sized gas mixtures. Here we report an approach to designing polymeric molecular sieve membranes via multi-covalent-crosslinking of blended bromomethyl polymer of intrinsic microporosity and Tröger's base, enabling simultaneously high permeability and selectivity. Ultra-selective gas separation is achieved via adjusting reaction temperature, reaction time and the oxygen concentration with occurrences of polymer chain scission, rearrangement and thermal oxidative crosslinking reaction. Upon a thermal treatment at 300 °C for 5 h, membranes exhibit an O/N, CO/CH and H/CH selectivity as high as 11.1, 154.5 and 813.6, respectively, transcending the state-of-art upper bounds. The design strategy represents a generalizable approach to creating molecular-sieving polymer membranes with enormous potentials for high-performance separation processes.
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http://dx.doi.org/10.1038/s41467-021-26379-5DOI Listing
October 2021

Measuring the ratio of femoral vein diameter to femoral artery diameter by ultrasound to estimate volume status.

BMC Cardiovasc Disord 2021 Oct 20;21(1):506. Epub 2021 Oct 20.

Department of Cardiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.

Background: Currently, the accepted effective method for assessing blood volume status, such as measuring central venous pressure (CVP) and mean pulmonary artery pressure (mPAP), is invasive. The purpose of this study was to explore the feasibility and validity of the ratio of the femoral vein diameter (FVD) to the femoral artery diameter (FAD) for predicting CVP and mPAP and to calculate the cut-off value for the FVD/FAD ratio to help judge a patient's fluid volume status.

Methods: In this study, 130 patients were divided into two groups: in group A, the FVD, FAD, and CVP were measured, and in group B, the FVD, FAD, and mPAP were measured. We measured the FVD and FAD by ultrasound. We monitored CVP by a central venous catheter and mPAP by a Swan-Ganz floating catheter. Pearson correlation coefficients were calculated. The best cut-off value for the FVD/FAD ratio for predicting CVP and mPAP was obtained according to the receiver operating characteristic (ROC) curve.

Results: The FVD/FAD ratio was strongly correlated with CVP (R = 0.87, P < 0.0000) and mPAP (R = 0.73, P < 0.0000). According to the ROC curve, an FVD/FAD ratio ≥ 1.495 had the best test characteristics to predict a CVP ≥ 12 cmHO, and an FVD/FAD ratio ≤ 1.467 had the best test characteristics to predict a CVP ≤ 10 cmHO. An FVD/FAD ratio ≥ 2.03 had the best test characteristics to predict an mPAP ≥ 25 mmHg. According to the simple linear regression curve of the FVD/FAD ratio and CVP, when the predicted CVP ≤ 5 cmHO, the FVD/FAD ratio was ≤ 0.854.

Conclusion: In this study, the measurement of the FVD/FAD ratio obtained via ultrasound was strongly correlated with CVP and mPAP, providing a non-invasive method for quickly and reliably assessing blood volume status and providing good clinical support.
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http://dx.doi.org/10.1186/s12872-021-02309-7DOI Listing
October 2021

The mediating role of gestational diabetes mellitus in the Associations of Maternal Prepregnancy Body Mass Index with Neonatal Birth Weight.

J Diabetes 2021 Oct 19. Epub 2021 Oct 19.

Department of Epidemiology, School of Public Health, Medical College of Soochow University, Suzhou, China.

Background: Both pre-pregnancy obesity and gestational diabetes mellitus (GDM) have been linked to adverse neonatal birth weight. However, the mediating role of GDM between pre-pregnancy obesity and neonatal birth weight is unclear.

Method: The cohort study included 17260 singleton pregnant women and their newborns. Participants' demographic characteristics, disease history, family history of the disease, and the perinatal outcomes were recorded. The association between maternal prepregnancy BMI status and small for gestational age (SGA), large for gestational age (LGA) neonates were analyzed using logistic regressions, before and after adjusting for covariates and GDM. The potential mediation of GDM on the association between pre-pregnancy BMI and adverse birth weight was examined.

Result: Multivariate logistic regression demonstrated that pre-pregnancy underweight women were more likely to deliver SGA neonates compared to those who had normal weights, whereas, pre-pregnancy obese pregnant women were more likely to have LGA neonates. The RMediation analyses illustrated that the mediation effect of GDM on the maternal pre-pregnancy BMI (continuous variable) and the risk of SGA wasn't significant, while the association between pre-pregnancy BMI and LGA was statistically mediated by GDM (95%CI of a*b: 0.009-0.051). The Iacobacci's (2012) method indicated that the maternal pre-pregnancy overweight (Zmediation= 2.418, p=0.015) and obesity (Zmediation= 2.165, p=0.030) on LGA were partially mediated by GDM, with an indirect effect of 16.3% and 13.1%, respectively.

Conclusion: Pre-pregnancy BMI was observed to be associated with SGA and LGA. The association of pre-pregnancy overweight and obesity with LGA was found to be partially mediated by GDM.
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http://dx.doi.org/10.1111/1753-0407.13233DOI Listing
October 2021

The Mechanism Action of German Chamomile () in the Treatment of Eczema: Based on Dose-Effect Weight Coefficient Network Pharmacology.

Front Pharmacol 2021 30;12:706836. Epub 2021 Sep 30.

Department of Pharmaceutics, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, China.

To determine the active ingredients in German chamomile volatile oil and the mechanism of action in the treatment of eczema, this study used two parameters (ingredient content and oil-water partition coefficient) and established a new network pharmacology method based on the dose-effect weight coefficient. Through the new network pharmacology method, we found that German chamomile volatile oil regulated T-cell lymphatic subpopulations to inhibit the Th17 cell differentiation signaling pathway. This resulted in a reduction of interleukin 17 (IL-17), thereby inhibiting the activation of the nuclear factor kappa beta (NF-κB) and MAPK pathways, decreasing the secretion of the pro-inflammatory factors (tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6)), and reducing inflammation. In this study, a new dose-effect relationship synergistic network pharmacology method was established to provide a new method for the screening of effective ingredients and pathways of drugs, and to provide a basis for the follow-up studies of German chamomile volatile oil in the treatment of eczema.
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http://dx.doi.org/10.3389/fphar.2021.706836DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515037PMC
September 2021

Unresected small lymph node assessment predicts prognosis for patients with pT3N0M0 thoracic esophageal squamous cell carcinoma.

World J Surg Oncol 2021 Oct 18;19(1):303. Epub 2021 Oct 18.

Department of Thoracic Surgery, Sichuan Cancer Hospital and Institution, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.

Background: The role of unresected small lymph nodes (LNs) which may contain metastases for thoracic esophageal squamous cell carcinoma (TESCC) has not been addressed. The aim of the study was to investigate the role of unresected small LNs assessment using computed tomography (CT) in prognostic estimates of pT3N0M0 TESCC patients.

Methods: Between January 2009 and December 2017, 294 patients who underwent esophagectomy with R0 resection at Sichuan Cancer Hospital were retrospectively examined, and the last follow-up time was July 2018. Patients were classified into CT-suspect and CT-negative groups according to the shortest diameter and the shape (axial ratio) of the unresected small LNs on preoperative CT. The Kaplan-Meier method was used to compare survival differences in prognostic factors. Univariate and multivariate analyses were performed to identify prognostic factors for survival and recurrence.

Results: Eighty-four patients (28.6%) were classified as CT-suspect group according to the diagnostic criteria; survival analysis suggested that CT-suspect group of patients had a relatively poorer prognosis (P<0.05). Cox regression analysis indicated that unresected small LNs status, tumor grade, and postoperative adjuvant therapy were independent prognostic factors for patients with pT3N0M0 TESCC (P<0.05). Further analysis shown the rates of total recurrence (TR) and locoregional recurrence (LR) in the CT-suspect group were significantly higher than that in the CT-negative group (TR, P<0.001; LR, P<0.001). Among the LRs, the rate of supraclavicular lymph node recurrence in the CT-suspect group was significantly higher than that in the CT-negative group (P<0.001).

Conclusions: Unresected small lymph node assessment is critically important and predict prognosis for pT3N0M0 TESCC patients.
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http://dx.doi.org/10.1186/s12957-021-02412-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8522218PMC
October 2021

Isolation of 2 simazine-degrading bacteria and development of a microbial agent for bioremediation of simazine pollution.

An Acad Bras Cienc 2021 6;93(suppl 3):e20210373. Epub 2021 Oct 6.

Pudong Agrotechnology Extension Center, No. 386, Pailou Road, 201201, Shanghai, China.

Simazine was one of the most commonly used herbicides and was widely used to control broadleaf weeds in agriculture and forestry. Its widespread use had caused wide public concern for its high ecological toxicity. In order to remove simazine residues, 2 strains capable of effectively degrading simazine were isolated from the soil and named SIMA-N5 and SIMA-N9. SIMA-N5 was identified as Bacillus licheniformis by 16SrRNA sequence analysis, and SIMA-N9 was Bacillus altitudinis. According to the degradation ratio of simazine in a certain period of time, the degradation ability of different strains was evaluated. The degradation efficiency of simazine (5 mg/L) by SIMA-N9 could reach about 98% in 5d, and the strain SIMA-N5 could reach 94% under the same conditions. In addition, the addition of Pennisetum rhizosphere soil during the process of degrading simazine by strain SIMA-N9 could effectively improve the degradation efficiency. The strain SIMA-N9 has been developed as a microbial agent for the bioremediation of simazine contamination in soil. The new microbial agent developed by using SIMA-N9 has achieved satisfactory application effects. Based on the research results already obtained in this study, it was considered that strain SIMA-N9 and its live bacterial agent could play an important role in bioremediation of simazine pollution. This study could not only provide a set of solutions to the simazine pollution, but also provide a reference for the treatment of other pesticide pollution.
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http://dx.doi.org/10.1590/0001-3765202120210373DOI Listing
October 2021

Formaldehyde induces ferritinophagy to damage hippocampal neuronal cells.

Toxicol Ind Health 2021 Oct 13:7482337211048582. Epub 2021 Oct 13.

The First Affiliated Hospital, Institute of Neurology, Hengyang Medical School, 145440University of South China, Hengyang, P. R. China.

Formaldehyde (FA) causes neurotoxicity and contributes to the occurrence of neurodegenerative diseases. However, the mechanism of FA-induced neurotoxicity has not been fully elucidated. Ferritinophagy, an autophagy process of ferritin mediated by the nuclear receptor coactivator 4 (NCOA4), is a potential mechanism of neurotoxicity. In this study, we explored whether ferritinophagy is associated with the neurotoxicity of FA. Our results showed that FA (50, 100, 200 μM; 24 h) exposure upregulated ferritinophagy in the mouse hippocampal neuronal HT22 cells, which was evidenced by the upregulated autophagic flux, the increased colocalizations of NCOA4 with ferritin heavy chain (FTH1) and NCOA4 with microtubule-associated protein 1 light chain-3B (LC3B), the augmented expression of NCOA4, and the reduced content of FTH1. We also found that FA (0.1, 1, and 10 μmol, i.c.v., 7d) administration boosted ferritinophagy in the hippocampus of Sprague-Dawley (SD) rats, which was demonstrated by the accumulated autophagosomes, the increased expressions of LC3II/I and NCOA4, and the decreased contents of p62 and FTH1 in the hippocampus. Further, we confirmed that inhibition of ferritinophagy by silencing the expression of NCOA4 decreased FA-induced toxic damage in HT22 cells. These results indicated that FA induces neurotoxicity by promoting ferritinophagy. Our findings suggest a potential mechanism insight into the FA-induced neurotoxicity, which in turn provides a new thought for the treatment of FA-related neurodegenerative diseases.
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http://dx.doi.org/10.1177/07482337211048582DOI Listing
October 2021

An Edaravone-Guided Design of a Rhodamine-Based Turn-on Fluorescent Probe for Detecting Hydroxyl Radicals in Living Systems.

Anal Chem 2021 Oct 13. Epub 2021 Oct 13.

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an 710127, P. R. China.

The hydroxyl radical (·OH), one of the reactive oxygen species (ROS) in biosystems, is found to be involved in many physiological and pathological processes. However, specifically detecting endogenous ·OH remains an outstanding challenge owing to the high reactivity and short lifetime of this radical. Herein, inspired by the scavenging mechanism of a neuroprotective drug edaravone toward ·OH, we developed a new ·OH-specific fluorescent probe . is a hybrid of rhodamine and edaravone and exploits a ·OH-specific 3-methyl-pyrazolone moiety to control its fluorescence behavior. itself is almost nonfluorescent in physiological conditions, which was attributed to the formation of a twisted intramolecular charge transfer (TICT) state upon photoexcitation and the acylation of its rhodamine nitrogen at the 3' position. However, upon a treatment with ·OH, its edaravone subunit was converted to the corresponding 2-oxo-3-(phenylhydrazono)-butanoic acid (OPB) derivative (to afford ), thus leading to a significant fluorescence increase (ca. 195-fold). shows a high sensitivity and selectivity to ·OH without interference from other ROS. has been utilized for imaging endogenous ·OH production in living cells and zebrafishes under different stimuli. Moreover, allows a high-contrast discrimination of cancer cells from normal ones by monitoring their different ·OH levels upon stimulation with β-Lapachone (β-Lap), an effective ROS-generating anticancer therapeutic agent. The present study provides a promising methodology for the construction of probes through a drug-guided approach.
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http://dx.doi.org/10.1021/acs.analchem.1c03877DOI Listing
October 2021

Clinical Characteristics of Pediatric Cases of COVID-19 in Hunan, China: A Retrospective, Multi-Center Case Series.

Front Pediatr 2021 24;9:665377. Epub 2021 Sep 24.

Department of Pediatrics, Changsha Central Hospital, University of South China, Changsha, China.

Aimed to investigate the epidemiological characteristics, clinical features, treatment, and short-term prognosis of COVID-19 in children. Retrospective analysis was conducted in 48 children with COVID-19 admitted to 12 hospitals in eight cities in Hunan province, China, from January 26, 2020 to June 30, 2020. Of the 48 cases, Familial clusters were confirmed for 46 children (96%). 16 (33%) were imported from other provinces. There were 11 (23%) asymptomatic cases. only 2 cases (4%) were severe. The most common symptom was fever ( = 20, 42%). Other symptoms included cough ( = 19, 40%), fatigue ( = 8, 17%), and diarrhea ( = 5, 10%). In the early stage, the total peripheral blood leukocytes count increased in 3(6%) cases and the lymphocytes count decreased in 5 (10%) cases. C-reactive protein and procalcitonin were elevated respectively in 3 (6%) cases and 2 (4%) cases. There were abnormal chest CT changes in 22 (46%) children, including 15 (68%) with patchy ground glass opacity, 5 (22%) with consolidation, and 2 (10%) with mixed shadowing. In addition to supportive treatment, antiviral therapy was received by 41 (85%) children, 11 (23%) patients were treated with antibiotics, and 2 (4%) were treated with methylprednisolone and intravenous immunoglobulin. Compared to 2 weeks follow-up, one child developed low fever and headache during the 4 weeks follow-up, 3 (6%) children had runny noses, one of them got mild cough, and 4 (12%) children had elevated white blood cells and lymphocytes. However, LDH and CK increased at 2 weeks and 4 weeks follow-up. 2 weeks follow-up identified normal chest radiographs in 33 (69%) pediatric patients. RT-PCR detection of SARS-CoV-2 was negative in all follow-up patients at 2 and 4 weeks follow-up. All 48 pediatric patients were visited by calling after 1 year of discharge. Most cases of COVID-19 in children in Hunan province were asymptomatic, mild, or moderate. Close family contact was the main route of infection. It appeared that the younger the patient, the less obvious their symptoms. Epidemiological history, nucleic acid test, and chest imaging were important tools for diagnosis in children.
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http://dx.doi.org/10.3389/fped.2021.665377DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8498596PMC
September 2021

Enhanced degradation of chloramphenicol through peroxymonosulfate and visible light over Z-scheme Photocatalysts: Synergetic performance and mechanism insights.

J Colloid Interface Sci 2021 Oct 2;608(Pt 1):322-333. Epub 2021 Oct 2.

School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address:

Effective removal of antibiotics in the environment can be a demanding issue concerning the ecosystem and human health. Photocatalysis and peroxymonosulfate (PMS) oxidation have become important methods to effectively remove stubborn pollutants. In this work, by integrating these two technologies, an efficient system for degrading chloramphenicol (CAP) in water was proposed. The system was constructed by coupling strontium-doped lanthanum cobaltate (LSCO5) with chlorine-doped carbon nitride (CGCN). By doping, the increase of oxygen vacancy and the adjustment of bandgap were realized. Photoluminescence and electrochemical impedance experiments showed that the heterojunction can promote electron transfer and photogenerated carrier separation. Under the synergistic effect of PMS oxidation and photocatalysis, the prepared composite with an optimal loading of 40% LSCO5 can degrade 95.6% of CAP within 20 min. Degradation experiments on different pollutants proved the versatility of the catalytic system. The enhanced degradation mechanism of CAP was explored based on the assessment of the degradation efficiency of CAP, electron paramagnetic resonance (EPR), and quenching experiments. Through liquid chromatography-mass spectrometry (LC-MS) analysis, a possible route for CAP degradation was also proposed. This research provides some inspiration for the remediation of polluted water with perovskite-based catalyst under the synergistic effect of PMS and photocatalysis.
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http://dx.doi.org/10.1016/j.jcis.2021.09.197DOI Listing
October 2021

Self-Stabilized Supramolecular Assemblies Constructed from PEGylated Dendritic Peptide Conjugate for Augmenting Tumor Retention and Therapy.

Adv Sci (Weinh) 2021 Oct 7:e2102741. Epub 2021 Oct 7.

Huaxi MR Research Center (HMRRC), Department of Radiology, National Clinical Research Center for Geriatrics, Frontiers Science Center for Disease-Related Molecular Network, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China.

Supramolecular self-assemblies of dendritic peptides with well-organized nanostructures have great potential as multifunctional biomaterials, yet the complex self-assembly mechanism hampers their wide exploration. Herein, a self-stabilized supramolecular assembly (SSA) constructed from a PEGylated dendritic peptide conjugate (PEG-dendritic peptide-pyropheophorbide a, PDPP), for augmenting tumor retention and therapy, is reported. The supramolecular self-assembly process of PDPP is concentration-dependent with multiple morphologies. By tailoring the concentration of PDPP, the supramolecular self-assembly is driven by noncovalent interactions to form a variety of SSAs (unimolecular micelles, oligomeric aggregates, and multi-aggregates) with different sizes from nanometer to micrometer. SSAs at 100 nm with a spherical shape possess extremely high stability to prolong blood circulation about 4.8-fold higher than pyropheophorbide a (Ppa), and enhance tumor retention about eight-fold higher than Ppa on day 5 after injection, which leads to greatly boosting the in vivo photodynamic therapeutic efficiency. RNA-seq demonstrates that these effects of SSAs are related to the inhibition of MET-PI3K-Akt pathway. Overall, the supramolecular self-assembly mechanism for the synthetic PEGylated dendritic peptide conjugate sheds new light on the development of supramolecular assemblies for tumor therapy.
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http://dx.doi.org/10.1002/advs.202102741DOI Listing
October 2021

Facile immobilization of ethylenediamine tetramethylene-phosphonic acid into UiO-66 for toxic divalent heavy metal ions removal: An experimental and theoretical exploration.

Sci Total Environ 2021 Oct 2;806(Pt 3):150652. Epub 2021 Oct 2.

School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address:

By the facile immobilization of ethylenediamine tetramethylene-phosphonic acid (EDTMPA) onto the surface and into the defects of UiO-66, a stable and efficient adsorbent named UiO-66-EDTMPA was obtained for the first time. In terms of removing aqueous heavy metal ions (Pb, Cd, Cu), the maximum adsorption capacities of UiO-66-EDTMPA reached 558.67, 271.34 and 210.89 mg/g, which were 8.77 (Pb), 5.63 (Cd) and 5.19 (Cu) times higher than raw UiO-66 respectively. The adsorption behavior of three heavy metal ions on UiO-66 and UiO-66-EDTMPA were investigated and compared through batch control experiments and theoretical studies. The main factors on adsorption progress (i.e., the dosage of EDTMPA, pH, ionic strength, co-existing ions, initial concentration, contact time, temperature) were explored, and the critical characterization (i.e., SEM, TEM, XRD, FT-IR, TG-DTG, XPS, N adsorption-desorption test) were performed. Molecular dynamics (MD) simulation (radial distribution functions (RDF) and mean square displacement (MSD)) were also applied to reveal the adsorption behavior. Besides, two new quantum chemical analyses (Hirshfeld surface and independent gradient model (IGM)) were introduced into the interaction analysis between UiO-66 and EDTMPA. The complete results showed that (1) where the hydrogen bond and (vdW) connect EDTMPA to UiO-66. (2) The coordination between O, N atoms of EDTMPA and heavy metal ions (Pb, Cd, Cu) resulted in spontaneous adsorption. (3) The adsorption behavior agreed with Langmuir and pseudo-second-order model, endothermic reaction. In addition, the desorption and reusability study showed promising stable and sustainable performance. This work has some guiding significance for the experimental and theoretical study of removing heavy metal ions from aqueous solutions by MOF or modified MOF materials.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150652DOI Listing
October 2021

Facile immobilization of ethylenediamine tetramethylene-phosphonic acid into UiO-66 for toxic divalent heavy metal ions removal: An experimental and theoretical exploration.

Sci Total Environ 2021 Oct 2;806(Pt 3):150652. Epub 2021 Oct 2.

School of Chemistry and Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. Electronic address:

By the facile immobilization of ethylenediamine tetramethylene-phosphonic acid (EDTMPA) onto the surface and into the defects of UiO-66, a stable and efficient adsorbent named UiO-66-EDTMPA was obtained for the first time. In terms of removing aqueous heavy metal ions (Pb, Cd, Cu), the maximum adsorption capacities of UiO-66-EDTMPA reached 558.67, 271.34 and 210.89 mg/g, which were 8.77 (Pb), 5.63 (Cd) and 5.19 (Cu) times higher than raw UiO-66 respectively. The adsorption behavior of three heavy metal ions on UiO-66 and UiO-66-EDTMPA were investigated and compared through batch control experiments and theoretical studies. The main factors on adsorption progress (i.e., the dosage of EDTMPA, pH, ionic strength, co-existing ions, initial concentration, contact time, temperature) were explored, and the critical characterization (i.e., SEM, TEM, XRD, FT-IR, TG-DTG, XPS, N adsorption-desorption test) were performed. Molecular dynamics (MD) simulation (radial distribution functions (RDF) and mean square displacement (MSD)) were also applied to reveal the adsorption behavior. Besides, two new quantum chemical analyses (Hirshfeld surface and independent gradient model (IGM)) were introduced into the interaction analysis between UiO-66 and EDTMPA. The complete results showed that (1) where the hydrogen bond and (vdW) connect EDTMPA to UiO-66. (2) The coordination between O, N atoms of EDTMPA and heavy metal ions (Pb, Cd, Cu) resulted in spontaneous adsorption. (3) The adsorption behavior agreed with Langmuir and pseudo-second-order model, endothermic reaction. In addition, the desorption and reusability study showed promising stable and sustainable performance. This work has some guiding significance for the experimental and theoretical study of removing heavy metal ions from aqueous solutions by MOF or modified MOF materials.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150652DOI Listing
October 2021

The genome of Chinese flowering cherry (Cerasus serrulata) provides new insights into Cerasus species.

Hortic Res 2020 Oct 1;7(1):165. Epub 2020 Oct 1.

Co-Innovation Center for the Sustainable Forestry in Southern China, College of Biology and the Environment; Cerasus Research Center, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China.

Cerasus serrulata is a flowering cherry germplasm resource for ornamental purposes. In this work, we present a de novo chromosome-scale genome assembly of C. serrulata by the use of Nanopore and Hi-C sequencing technologies. The assembled C. serrulata genome is 265.40 Mb across 304 contigs and 67 scaffolds, with a contig N50 of 1.56 Mb and a scaffold N50 of 31.12 Mb. It contains 29,094 coding genes, 27,611 (94.90%) of which are annotated in at least one functional database. Synteny analysis indicated that C. serrulata and C. avium have 333 syntenic blocks composed of 14,072 genes. Blocks on chromosome 01 of C. serrulata are distributed on all chromosomes of C. avium, implying that chromosome 01 is the most ancient or active of the chromosomes. The comparative genomic analysis confirmed that C. serrulata has 740 expanded gene families, 1031 contracted gene families, and 228 rapidly evolving gene families. By the use of 656 single-copy orthologs, a phylogenetic tree composed of 10 species was constructed. The present C. serrulata species diverged from Prunus yedoensis ~17.34 million years ago (Mya), while the divergence of C. serrulata and C. avium was estimated to have occurred ∼21.44 Mya. In addition, a total of 148 MADS-box family gene members were identified in C. serrulata, accompanying the loss of the AGL32 subfamily and the expansion of the SVP subfamily. The MYB and WRKY gene families comprising 372 and 66 genes could be divided into seven and eight subfamilies in C. serrulata, respectively, based on clustering analysis. Nine hundred forty-one plant disease-resistance genes (R-genes) were detected by searching C. serrulata within the PRGdb. This research provides high-quality genomic information about C. serrulata as well as insights into the evolutionary history of Cerasus species.
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http://dx.doi.org/10.1038/s41438-020-00382-1DOI Listing
October 2020

Novel β-mannanase/GLP-1 fusion peptide high effectively ameliorates obesity in a mouse model by modifying balance of gut microbiota.

Int J Biol Macromol 2021 Sep 28;191:753-763. Epub 2021 Sep 28.

State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. Electronic address:

We constructed a novel β-mannanase/GLP-1 fusion peptide, termed MGLP_1, and evaluated its ability to ameliorate obesity in a high-fat/high-sugar diet (HFSD)-induced mouse model. Eight-wk MGLP_1 treatment notably reduced obesity, as reflected by significant changes of body weight, serum triglyceride level, fatty liver and adipose tissue distribution. Amelioration of HFSD-induced gut dysbiosis by MGLP_1 was evidenced by reduced abundance ratio of bacterial phyla Firmicutes to Bacteroidetes, enhanced abundance of beneficial probiotic genera (Bifidobacterium, Lachnospiraceae, Ileibacterium), and reduced abundance of harmful genera (Clostridium, Romboutsia). Mechanisms of weight loss were investigated by comparing effects of treatment with MGLP_1 vs. prebiotics manno-oligosaccharides (MOS). MGLP_1 ameliorated gut microbiota imbalance by enhancing carbohydrate catabolism, whereas MOS promoted glycan synthesis and metabolism. Our findings, taken together, indicate that MGLP_1 fusion peptide has strong potential for amelioration of obesity by modifying relationships between gut microbiota and lipid and glucose metabolism.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.09.150DOI Listing
September 2021

Regulation and Evasion of Host Immune Response by African Swine Fever Virus.

Front Microbiol 2021 8;12:698001. Epub 2021 Sep 8.

Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.

African swine fever (ASF) is an acute lethal hemorrhagic viral disease in domestic pigs and wild boars; is widely epidemic in Africa, Europe, Asia, and Latin America; and poses a huge threat to the pig industry worldwide. ASF is caused by the infection of the ASF virus (ASFV), a cytoplasmic double-stranded DNA virus belonging to the family. Here, we review how the virus regulates the host immune response and its mechanisms at different levels, including interferon modulation, inflammation, apoptosis, antigen presentation, and cellular immunity.
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http://dx.doi.org/10.3389/fmicb.2021.698001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457549PMC
September 2021

Fear-of-intimacy-mediated zinc transport controls fat body cell dissociation through modulating Mmp activity in Drosophila.

Cell Death Dis 2021 Sep 25;12(10):874. Epub 2021 Sep 25.

School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui, China.

Matrix metalloproteinases (Mmps) are pivotal extracellular proteinases that have been implicated in tumour invasion and metastasis. Drosophila fat body is important for energy storage and utilization, as well as biosynthetic and metabolic activities. The fat body undergoes remodelling during metamorphosis which is characterized by the dissociation of the fat body into individual cells. Mmps play important roles in the regulation of fat body cell dissociation. Here we show that a zinc transporter fear-of-intimacy (foi) is necessary for the cell dissociation of fat body in Drosophila. The progression of fat body cell dissociation was delayed by fat body-specific foi knockdown while it was accelerated by foi overexpression (OE). In essence, these phenotypes are closely associated with intracellular zinc homeostasis, which can be modulated by dietary zinc intervention or genetic modulation of other zinc transporters. Further study indicated that Mmp1 and Mmp2 levels could be transcriptionally regulated by zinc in vivo. Consistently, the retarded fat body cell dissociation caused by Mmp1 or Mmp2 RNAi could be regulated by modulating the expression of foi. Further, by using Drosophila models of malignant tumour Rafscrib and Raslgl, we showed that the tumour growth, invasion and migration could be markedly inhibited by foi knockdown. These findings demonstrate a close connection between zinc levels and cell dissociation in vivo, and also suggest that manipulation of zinc levels may provide a novel therapeutic strategy for cancer.
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http://dx.doi.org/10.1038/s41419-021-04147-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464599PMC
September 2021

Signatures of Multi-Omics Reveal Distinct Tumor Immune Microenvironment Contributing to Immunotherapy in Lung Adenocarcinoma.

Front Immunol 2021 3;12:723172. Epub 2021 Sep 3.

Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.

Background: Lung adenocarcinoma (LUAD) contains a variety of genomic and epigenomic abnormalities; the effective tumor markers related to these abnormalities need to be further explored.

Methods: Clustering analysis was performed based on DNA methylation (MET), DNA copy number variation (CNV), and mRNA expression data, and the differences in survival and tumor immune microenvironment (TIME) between subtypes were compared. Further, we evaluated the signatures in terms of both prognostic value and immunological characteristics.

Results: There was a positive correlation between MET and CNV in LUAD. Integrative analysis of multi-omics data from 443 samples determined molecular subtypes, iC1 and iC2. The fractions of CD8+ T cells and activated CD4+ T cells were higher, the fraction of Tregs was lower, and the expression level of programmed death-ligand 1 (PD-L1) was higher in iC2 with a poor prognosis showing a higher TIDE score. We selected , , and as signatures of molecular subtypes to build a prognostic risk model and divided patients into high-risk group and low-risk group representing poor prognosis and good prognosis, respectively, which were validated in 180 patients with LUAD. Further, the low-risk group with lower TIDE score had more infiltrating immune cells. In 100 patients with LUAD, the high-risk group with an immunosuppressive state had a higher expression of PD-L1 and lower counts of CD8+ T cells and dendritic cells.

Conclusions: These findings demonstrated that combined multi-omics data could determine molecular subtypes with significant differences of prognosis and TIME in LUAD and suggested potent utility of the signatures to guide immunotherapy.
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http://dx.doi.org/10.3389/fimmu.2021.723172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446514PMC
September 2021

Advances in Chemical Constituents, Clinical Applications, Pharmacology, Pharmacokinetics and Toxicology of .

Front Pharmacol 2021 2;12:656335. Epub 2021 Sep 2.

College of Ethnomedicine, Chengdu University of Traditional Chinese Medicine, Sichuan, China.

Dengzhanxixin (DZXX), the dried whole plant of (Vaniot) Hand.-Mazz., belonging to and first published in by Lan Mao in the Ming Dynasty (1368 AD-1644 AD), is included in Medicinal Materials and Decoction Pieces of the 2020 edition of the Pharmacopeia of the . Its main chemical components are flavonoids that mainly include flavonoid, flavonols, dihydroflavones, flavonol glycosides, flavonoid glycosides, coffee acyl compounds, and other substances, such as volatile oil compounds, coumarins, aromatic acids, pentacyclic terpenoids, phytosterols, and xanthones. Among them, scutellarin and 1,5-dicoffeoylquininic acid are the main active components of DZXX. DZXX has pharmacological effects, such as improving cerebral and cerebrovascular ischemia, increasing blood flow, inhibiting platelet aggregation, promoting antithrombotic formation, improving microcirculation, reducing blood viscosity, protecting optic nerves, exhibiting anti-inflammatory properties, scavenging free radicals, and eliciting antioxidant activities. It is widely used in the treatment of cardiovascular and cerebrovascular ischemic diseases, kidney diseases, liver diseases, diabetic complications, and glaucoma. Pharmacokinetic studies have shown that the active components of DZXX have a low bioavailability and a high elimination rate in vivo. Nevertheless, its utilization can be improved through liposome preparation and combination with other drugs. Acute and subacute toxicity studies have shown that DZXX is a safe medicinal material widely used in clinical settings. However, its target and drug action mechanism are unclear because of the complexity of its composition. In this paper, the clinical application and pharmacological toxicology of DZXX are reviewed to provide a reference for further studying its active components and action mechanism.
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http://dx.doi.org/10.3389/fphar.2021.656335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443777PMC
September 2021

Methodological and reporting quality of pediatric clinical practice guidelines: a systematic review.

Ann Transl Med 2021 Aug;9(15):1258

National Clinical Research Center for Child Health and Disorders, Evidence-Based Medicine Center, Basic Medical Sciences, Lanzhou University, Lanzhou, China.

Background: Pediatric clinical practice guidelines (CPGs) can provide systematically developed clinical recommendations to guide pediatric clinicians and patients making decisions. This study aims to assess the methodological and reporting quality of pediatric CPGs.

Methods: We performed a systematic literature search of MEDLINE from 1 January 1990 to 2 April 2020 to identify pediatric CPGs published in the ten highest-impact pediatric journals and four highest-impact general medical journals. Two researchers evaluated the methodological and reporting quality of pediatric CPGs using the Appraisal of Guidelines Research and Evaluation (AGREE) II instrument and Reporting Items for Practice Guidelines in Health care (RIGHT) checklist. We calculated the mean AGREE II scores and compliance to RIGHT overall, and for each domain of the respective tools. We compared the methodological and reporting quality by different time periods and calculated the correlation between the AGREE II score and compliance to RIGHT.

Results: A total of 159 pediatric CPGs were identified. The mean (± standard deviation) scores for the six domains of the AGREE II instrument were as follows: scope and purpose 74.5%±14.2%, stakeholder involvement 42.7%±16.2%, rigour of development 18.7%±14.2%, clarity of presentation 56.5%±17.0%, applicability 8.9%±12.7% and editorial independence 25.2%±34.6%. The overall assessment score was 37.8%±12.4%. The mean compliance to RIGHT items in the seven domains of the checklist were: basic information 73.6%±14.9%, background 67.1%±16.4%, evidence 32.7%±27.2%, recommendations 32.4%±22.5%, review and quality assurance 22.9%±40.4%, funding and declaration and management of interests 24.1%±36.3%, and other information 45.3%±30.1%. The overall reporting rate for RIGHT was 46.4%±16.6%. Both the AGREE II scores and RIGHT reporting rates increased over time. We found a high positive correlation between AGREE II scores and RIGHT reporting compliance (r=0.645, P<0.001).

Discussion: The methodological and reporting quality of pediatric CPGs have improved over time, but remain still suboptimal and needs to be further improved. An international database of pediatric guidelines is urgently needed to identify and promote high-quality guidelines and guide clinical practice in pediatrics.
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http://dx.doi.org/10.21037/atm-21-2686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421971PMC
August 2021

Clinical Features of Spontaneous Regression of Retinopathy of Prematurity in China: A 5-Year Retrospective Case Series.

Front Med (Lausanne) 2021 31;8:731421. Epub 2021 Aug 31.

Department of Ophthalmology, Eye Institute of Chinese PLA, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

The aim of this study is to explore the clinical features of spontaneous regression of retinopathy of prematurity (ROP) in China, including fundus appearance, time course, and affecting factors. Data of pediatric patients in whom ROP spontaneously regressed without treatment were collected, including general demographics, medical history, zones and stages of ROP, and changes of fundus appearance. The fundus manifestations of spontaneous regression in ROP were systematically summarized. Meanwhile, the time course of spontaneous regression in ROP was further analyzed, including the onset time, completion time, and duration of regression, which were all compared across different ROP zones and stages. The associated factors were analyzed by survival analysis for their correlation with delayed regression for the first time. Two hundred thirty-seven eyes of 237 pediatric patients were included. The fundus manifestations of regression differed across stages. Lesions gradually subsided, and the retinal vessels gradually vascularized completely. However, despite ROP regression, some abnormalities remained. We observed avascular retina in the temporal periphery (19.0%), increased vascular branching (6.8%), retinal pigmentary changes (6.8%), and smaller angle between the upper and lower temporal retinal vessel trunks (3.0%). Acute ROP started to regress at a median 40 weeks of postmenstrual age (PMA) and completely regressed by median 49.0 weeks of PMA. The median duration for regression was 8.5 weeks. The zone II ROP and stage 3 ROP had a later time for onset and completion of regression, and longer duration. Anemia and retinal hemorrhage (RH) were identified as independent risk factors for delayed regression by survival analysis. During spontaneous regression, the fundus appearance is diverse, and the retinal vessels gradually vascularized completely. The time course of regression differs depending on the ROP zone and stage. Anemia and RH are independent risk factors for delayed regression. Further research of the natural course of the regression of ROP is needed to help design effective screening and follow-up plans.
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http://dx.doi.org/10.3389/fmed.2021.731421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8439357PMC
August 2021

Identification of the Ferroptosis-Associated Gene Signature to Predict the Prognostic Status of Endometrial Carcinoma Patients.

Comput Math Methods Med 2021 7;2021:9954370. Epub 2021 Sep 7.

Department of Obstetrics and Gynecology, Putuo People's Hospital, Tongji University School of Medicine, Shanghai, China.

Endometrial carcinoma (EC) is one of the most common gynecological carcinomas. As previously described, ferroptosis was reported to exhibit a significant association with the development of malignant neoplasms. Nevertheless, there are few studies towards the association between the implication of ferroptosis-related genes (FRGs) and the prognostic status of patients with EC. Our study demonstrated that ferroptosis-related genes were evidently differently expressed in EC. Further analysis showed that SLC7A11, SAT1, CDKN1A, and TP5MC3 expression was linked to the low stage, grade of pTNM, and longer survival time. Bioinformatics analysis demonstrated that these ferroptosis-related regulators played a crucial role in EC by modulating multiple biological processes, such as cell cycle, citrate cycle (TCA cycle), metabolism-related pathways, ERK activation, p53 signaling pathway, cellular senescence, TAp63 pathway, and Notch signaling pathway. Of note, our results showed that ATP5MC3, CDKN1A, and SLC7A11 expression was dramatically positively related with the tumor mutational burden (TMB) score in EC. However, we did not observe a significant correlation between SAT1 and the TMB score in EC. These findings for the first time demonstrated that ferroptosis was displayed crucially in EC progression. We speculated that our findings offered novel targets and strategies for personalized treatment.
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http://dx.doi.org/10.1155/2021/9954370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440105PMC
September 2021

Microbial Communities and Physicochemical Characteristics of Traditional Dajiang and Sufu in North China Revealed by High-Throughput Sequencing of 16S rRNA.

Front Microbiol 2021 30;12:665243. Epub 2021 Aug 30.

Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China.

The process of soybean fermentation has been practiced for more than 3,000 years. Although Dajiang and Sufu are two popular fermented soybean products consumed in North China, limited information is available regarding their microbial composition. Hence, the current study sought to investigate, and compare, the physicochemical indicators and microbial communities of traditional Dajiang and Sufu. Results showed that the titratable acidity (TA), and salinity, as well as the lactic acid, and malic acid contents were significantly higher in Sufu samples compared to Dajiang. Furthermore, Sufu samples contain abundant sucrose and fructose, while the acetic acid content was lower in Sufu compared to Dajiang samples. Moreover, the predominant bacterial phyla in Dajiang and Sufu samples were Firmicutes and Proteobacteria, while the major genera comprise , , , and . Moreover, Dajiang samples also contained abundant , and spp., while , , , , , , and spp. were abundant in Sufu samples. At the species level, , , , , , , and represented the major species in Dajiang, while sp., , and were the predominant species in Sufu. Acetic acid and sucrose were found to be the primary major physicochemical factor influencing the bacterial communities in Dajiang and Sufu, respectively. Furthermore, is strongly correlated with lactic acid levels, is positively correlated with acetic acid levels, while and are strongly, and positively, correlated with malic acid. Following analysis of carbohydrate and amino acid metabolism in all samples, cysteine and methionine metabolism, as well as fatty acid biosynthesis-related genes are upregulated in Dajiang compared to Sufu samples. However, such as the , , and , as potentially foodborne pathogens, existed in Dajang and Sufu samples. Cumulatively, these results suggested that Dajiang and Sufu have unique bacterial communities that influence their specific characteristics. Hence, the current study provides insights into the microbial community composition in Dajiang and Sufu samples, which may facilitate the isolation of functional bacterial species suitable for Dajiang and Sufu production, thus improving their production efficiency.
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http://dx.doi.org/10.3389/fmicb.2021.665243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435802PMC
August 2021

Desorption Effects and Laws of Multiscale Gas-Bearing Coal with Different Degrees of Metamorphism.

ACS Omega 2021 Aug 19;6(34):22114-22125. Epub 2021 Aug 19.

State Key Laboratory Cultivation Base for Gas Geology and Gas Control, Henan Polytechnic University, Jiaozuo, Henan 454003, China.

Understanding gas desorption effects and laws of coal mass under different conditions is essential for the effective exploration of gas emission in underground coal mines, prediction and prevention of coal and gas outburst, accurate detection of gas [coal methane (CBM)] content in coal seams, and prediction of CBM productivity. Using a self-developed test platform, we simulated gas adsorption and desorption and performed physical simulation tests. Based on these tests, we investigated the differences in the total amount of gas desorbed, desorption rate, and initial amount of gas desorbed by long-flame coal, coking coal, meager-lean coal, and anthracite on different scales under different gas pressures. Two methods are used for compensating gas loss, namely, the method and the power function method, as stipulated in the current Standards for Determination of Gas Content in Coal Seams in China. By combining these two methods, we analyzed the applicability of these two compensation methods in coal on different scales with varying degrees of metamorphism under gas pressures. The results demonstrated that (1) under the same gas adsorption pressure, the cumulative total amount of gas desorbed per unit mass within 90 min for the four kinds of coal samples increases with the degree of metamorphism. Changes in the cumulative amount of gas desorbed per unit mass and the desorption rate with the degree of metamorphism vary with stages. Notably, a higher adsorption pressure leads to a more obvious stage change. (2) Under the same gas adsorption pressure, the cumulative total amount of gas desorbed per unit mass and the desorption rate of coal with the same degree of metamorphism are inversely proportional to the size of the coal sample. This indicates significant scale effects. The larger the degree of metamorphism and gas adsorption pressure, the more significant are the scale effects of gas desorption. (3) For coal with the same degree of metamorphism, the higher gas adsorption pressure leads to a larger cumulative total amount of gas desorbed and a higher desorption rate throughout the desorption process and a larger proportion of the cumulative amount of gas desorbed in the initial stage. The smaller the size of the coal sample, the more obvious the pressure effects of gas desorption are. (4) For coal samples with the same degree of metamorphism, when the gas content in coal seams is kept constant, the larger the size of the coal sample, the smaller the actual gas loss is. Moreover, a higher gas content in coal seams results in a greater gas loss and a larger calculation error for gas loss. Compared with the method, the power function method reveals a smaller deviation between the calculated gas loss and the actual gas loss, which is found to be more accurate. A larger size coal sample results in higher accuracy in the calculated gas loss.
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http://dx.doi.org/10.1021/acsomega.1c02706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8412902PMC
August 2021

Spleen Radiomics Signature: A Potential Biomarker for Prediction of Early and Late Recurrences of Hepatocellular Carcinoma After Resection.

Front Oncol 2021 13;11:716849. Epub 2021 Aug 13.

The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China.

Objectives: To explore the usefulness of spleen radiomics features based on contrast-enhanced computed tomography (CECT) in predicting early and late recurrences of hepatocellular carcinoma (HCC) patients after curative resection.

Methods: This retrospective study included 237 HCC patients who underwent CECT and curative resection between January 2006 to January 2016. Radiomic features were extracted from CECT images, and then the spleen radiomics signatures and the tumor radiomics signatures were built. Cox regression analysis was performed to identify the independent risk factors of early and late recurrences. Then, multiple models were built to predict the recurrence-free survival of HCC after resection, and the incremental value of the radiomics signature to the clinicopathologic model was assessed and validated. Kaplan-Meier survival analysis was used to assess the association of the models with RFS.

Results: The spleen radiomics signature was independent risk factor of early recurrence of HCC. The mixed model that integrated microvascular invasion, tumor radiomics signature and spleen radiomics signature for the prediction of early recurrence achieved the highest C-index of 0.780 (95% CI: 0.728,0.831) in the primary cohort and 0.776 (95% CI: 0.716,0.836) in the validation cohort, and presented better predictive performance than clinicopathological model and combined model. In the analysis of late recurrence, the spleen radiomics signature was the only prognostic factor associated with late recurrence of HCC.

Conclusions: The identified spleen radiomics signatures are prognostic factors of both early and late recurrences of HCC patients after surgery and improve the predictive performance of model for early recurrence.
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http://dx.doi.org/10.3389/fonc.2021.716849DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414994PMC
August 2021

Microdroplet confined assembly enabling the scalable synthesis of titania supported ultrasmall low-valent copper catalysts for efficient photocatalytic activation of peroxymonosulfate.

Nanoscale 2021 Aug 4;13(32):13764-13775. Epub 2021 Aug 4.

Particle Engineering Laboratory, School of Chemical and Environmental Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, Jiangsu 215123, China.

The synthesis of highly dispersed low-valent copper catalysts is very challenging because they are prone to oxidation and sintering. Herein, scalable synthesis of ultrafine Cu(0)/Cu(i) catalysts supported on mesoporous titania microspheres is enabled by a one-step microdroplet confined assembly method. The extremely fast solute assembly in the microdroplet induces excellent metal precursor dispersion, reduces sol-gel crosslinking, and creates wrinkled microspheres with surface crusts and hollow cavities. This structural architecture allows the generation of an inner reductive gas environment during calcination in air to reduce Cu(ii) and create oxygen vacancy (O) sites in titania. The obtained catalysts exhibit excellent performance in the photocatalytic activation of peroxymonosulfate (PMS) for pollutant degradation. The Cu(0) species with a surface plasmon resonance effect and O-rich anatase facilitate efficient solar light utilization and charge separation. The intimate interface between Cu(i)/Cu(0) and anatase enables fast electron transfer and timely copper redox cycling to promote the activation of PMS.
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http://dx.doi.org/10.1039/d1nr03535eDOI Listing
August 2021

Visual Impairments Are Associated With Retinal Microvascular Density in Patients With Parkinson's Disease.

Front Neurosci 2021 12;15:718820. Epub 2021 Aug 12.

Department of Ophthalmology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Objective: This study aimed to evaluate retinal microvascular density in patients with Parkinson's disease (PD) and its correlation with visual impairment.

Methods: This cross-sectional study included 24 eyes of 24 patients with PD and 23 eyes of 23 healthy controls. All participants underwent ophthalmic examination, visual evoked potential (VEP) test, 25-item National Eye Institute Visual Function Questionnaire (NEI VFQ-25), and optical coherence tomography angiography (OCTA) examination. The correlation between retinal microvascular density and visual parameter was evaluated using Spearman correlation analysis, and the area under receiver operating characteristic curve (AUROC) was calculated.

Results: Parkinson's disease patients had prolonged P100 latency ( = 0.041), worse vision-related quality of life (composite score and 3 of 12 subscales in NEI VFQ-25), and decreased vessel density (VD) in all sectors of 3-mm-diameter region (all < 0.05) compared with healthy controls. There were no statistical differences in the ganglion cell-inner plexiform layer (GCIPL) thickness and retinal nerve fiber layer (RNFL) thickness between the two groups. A negative correlation was found between P100 latency and nasal and superior sectors of macular VD in a 3-mm-diameter region ( = -0.328, = 0.030; = -0.302, and = 0.047, respectively). Macular VD in a 3-mm-diameter region showed diagnostic capacities to distinguish PD patients from healthy controls (AUROCs, ranging from 0.655 to 0.723).

Conclusion: This study demonstrated that decreased retinal microvascular density was correlated with visual impairment in PD patients. Retinal microvasculature change may occur earlier than visual decline and retinal structure change and has the potential to be a promising diagnostic marker for early PD.
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http://dx.doi.org/10.3389/fnins.2021.718820DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8406760PMC
August 2021

Surface Defect Engineering on Perovskite Oxides as Efficient Bifunctional Electrocatalysts for Water Splitting.

ACS Appl Mater Interfaces 2021 Sep 1;13(36):42852-42860. Epub 2021 Sep 1.

College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, China.

The design of high-performance and cost-effective electrocatalysts for water splitting is of prime importance for efficient and sustainable hydrogen production. In this work, a surface defect engineering method is developed for optimizing the electrocatalytic activity of perovskite oxides for water electrolysis. A typical ferrite-based perovskite oxide material LaSrCoFeO (LSCF) is used and regulated by selective acid etching. The optimal parameters for the surface treatment are identified. An efficient bifunctional perovskite oxide, denoted LSCF-30, is prepared by selectively corroding the A-site Sr element in the surface region, which is found to not only increase the exposure and decrease the coordination of B-site metals but also effectively modulate the electronic structure of these metals. The crystal lattice of the perovskite bulk is kept constant during surface engineering, which ensures the structural stability of the perovskite catalyst. The findings demonstrate an effective strategy of surface defect engineering in enhancing the performance of perovskite oxide electrocatalysts for water splitting.
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http://dx.doi.org/10.1021/acsami.1c11895DOI Listing
September 2021

Delivery of therapeutic oligonucleotides in nanoscale.

Bioact Mater 2022 Jan 12;7:292-323. Epub 2021 Jun 12.

School of Biomedical Engineering, Sun Yat-sen University, Guangzhou, 510006, Guangdong, China.

Therapeutic oligonucleotides (TOs) represent one of the most promising drug candidates in the targeted cancer treatment due to their high specificity and capability of modulating cellular pathways that are not readily druggable. However, efficiently delivering of TOs to cancer cellular targets is still the biggest challenge in promoting their clinical translations. Emerging as a significant drug delivery vector, nanoparticles (NPs) can not only protect TOs from nuclease degradation and enhance their tumor accumulation, but also can improve the cell uptake efficiency of TOs as well as the following endosomal escape to increase the therapeutic index. Furthermore, targeted and on-demand drug release of TOs can also be approached to minimize the risk of toxicity towards normal tissues using stimuli-responsive NPs. In the past decades, remarkable progresses have been made on the TOs delivery based on various NPs with specific purposes. In this review, we will first give a brief introduction on the basis of TOs as well as the action mechanisms of several typical TOs, and then describe the obstacles that prevent the clinical translation of TOs, followed by a comprehensive overview of the recent progresses on TOs delivery based on several various types of nanocarriers containing lipid-based nanoparticles, polymeric nanoparticles, gold nanoparticles, porous nanoparticles, DNA/RNA nanoassembly, extracellular vesicles, and imaging-guided drug delivery nanoparticles.
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http://dx.doi.org/10.1016/j.bioactmat.2021.05.038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379367PMC
January 2022
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